JP2013519698A5 - - Google Patents

Info

Publication number

JP2013519698A5

JP2013519698A5 JP2012553263A JP2012553263A JP2013519698A5 JP 2013519698 A5 JP2013519698 A5 JP 2013519698A5 JP 2012553263 A JP2012553263 A JP 2012553263A JP 2012553263 A JP2012553263 A JP 2012553263A JP 2013519698 A5 JP2013519698 A5 JP 2013519698A5

Authority

JP

Japan

Prior art keywords

recombinant protein

hydrophobic side

protein according

side chain

amino acids

Prior art date

2011-02-09

Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)

Granted

Links

• Espacenet
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• 108010054218 Factor VIII Proteins 0.000 claims description 13
• 102000001690 Factor VIII Human genes 0.000 claims description 13
• 229960000301 factor viii Drugs 0.000 claims description 13
• 101000911390 Homo sapiens Coagulation factor VIII Proteins 0.000 claims description 11
• 230000002209 hydrophobic effect Effects 0.000 claims description 11
• 102100026735 Coagulation factor VIII Human genes 0.000 claims description 8
• 229920001477 hydrophilic polymer Polymers 0.000 claims description 5
• 102000015081 Blood Coagulation Factors Human genes 0.000 claims description 3
• 108010039209 Blood Coagulation Factors Proteins 0.000 claims description 3
• SQVRNKJHWKZAKO-UHFFFAOYSA-N beta-N-Acetyl-D-neuraminic acid Natural products CC(=O)NC1C(O)CC(O)(C(O)=O)OC1C(O)C(O)CO SQVRNKJHWKZAKO-UHFFFAOYSA-N 0.000 claims description 3
• 239000003114 blood coagulation factor Substances 0.000 claims description 3
• SQVRNKJHWKZAKO-OQPLDHBCSA-N sialic acid Chemical compound CC(=O)N[C@@H]1[C@@H](O)C[C@@](O)(C(O)=O)OC1[C@H](O)[C@H](O)CO SQVRNKJHWKZAKO-OQPLDHBCSA-N 0.000 claims description 3
• 208000031220 Hemophilia Diseases 0.000 claims description 2
• 208000009292 Hemophilia A Diseases 0.000 claims description 2
• 230000004913 activation Effects 0.000 claims description 2
• 102000007056 Recombinant Fusion Proteins Human genes 0.000 claims 10
• 108010008281 Recombinant Fusion Proteins Proteins 0.000 claims 10
• 239000008194 pharmaceutical composition Substances 0.000 claims 3
• 102000004169 proteins and genes Human genes 0.000 claims 2
• 108090000623 proteins and genes Proteins 0.000 claims 2
• 102000003838 Sialyltransferases Human genes 0.000 claims 1
• 108090000141 Sialyltransferases Proteins 0.000 claims 1
• 239000002253 acid Substances 0.000 claims 1
• 125000001931 aliphatic group Chemical group 0.000 claims 1
• 238000006555 catalytic reaction Methods 0.000 claims 1
• 230000002950 deficient Effects 0.000 claims 1
• 235000014113 dietary fatty acids Nutrition 0.000 claims 1
• 229940079593 drug Drugs 0.000 claims 1
• 239000003814 drug Substances 0.000 claims 1
• 229930195729 fatty acid Natural products 0.000 claims 1
• 239000000194 fatty acid Substances 0.000 claims 1
• 150000004665 fatty acids Chemical class 0.000 claims 1
• 238000004519 manufacturing process Methods 0.000 claims 1
• 150000001413 amino acids Chemical class 0.000 description 23
• FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 description 4
• 210000004027 cell Anatomy 0.000 description 4
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• 238000012217 deletion Methods 0.000 description 4
• 230000004989 O-glycosylation Effects 0.000 description 3
• 102000057593 human F8 Human genes 0.000 description 3
• 229960000900 human factor viii Drugs 0.000 description 3
• -1 for example Polymers 0.000 description 2
• 239000012634 fragment Substances 0.000 description 2
• 150000004676 glycans Chemical class 0.000 description 2
• 238000000034 method Methods 0.000 description 2
• 229920001542 oligosaccharide Polymers 0.000 description 2
• 150000002482 oligosaccharides Chemical class 0.000 description 2
• 239000013612 plasmid Substances 0.000 description 2
• 102000009027 Albumins Human genes 0.000 description 1
• 108010088751 Albumins Proteins 0.000 description 1
• 241000699802 Cricetulus griseus Species 0.000 description 1
• 241001465754 Metazoa Species 0.000 description 1
• 108010022394 Threonine synthase Proteins 0.000 description 1
• 125000003275 alpha amino acid group Chemical group 0.000 description 1
• 239000002299 complementary DNA Substances 0.000 description 1
• 230000001268 conjugating effect Effects 0.000 description 1
• 102000004419 dihydrofolate reductase Human genes 0.000 description 1
• 238000006911 enzymatic reaction Methods 0.000 description 1
• 239000013613 expression plasmid Substances 0.000 description 1
• 230000013595 glycosylation Effects 0.000 description 1
• 238000006206 glycosylation reaction Methods 0.000 description 1
• 230000004048 modification Effects 0.000 description 1
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• 210000001672 ovary Anatomy 0.000 description 1
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