JP2013513812A5 - - Google Patents
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- Publication number
- JP2013513812A5 JP2013513812A5 JP2012543890A JP2012543890A JP2013513812A5 JP 2013513812 A5 JP2013513812 A5 JP 2013513812A5 JP 2012543890 A JP2012543890 A JP 2012543890A JP 2012543890 A JP2012543890 A JP 2012543890A JP 2013513812 A5 JP2013513812 A5 JP 2013513812A5
- Authority
- JP
- Japan
- Prior art keywords
- polypeptide
- sample
- cell sample
- isolated cell
- binding
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000000523 sample Substances 0.000 claims 10
- 239000011230 binding agent Substances 0.000 claims 9
- 229920001184 polypeptide Polymers 0.000 claims 9
- 102000007066 Prostate-Specific Antigen Human genes 0.000 claims 5
- 108010072866 Prostate-Specific Antigen Proteins 0.000 claims 5
- 102000001307 androgen receptors Human genes 0.000 claims 5
- 108010080146 androgen receptors Proteins 0.000 claims 5
- 238000004166 bioassay Methods 0.000 claims 5
- 239000012472 biological sample Substances 0.000 claims 5
- 206010060862 Prostate cancer Diseases 0.000 claims 4
- 101700032382 MCM2 Proteins 0.000 claims 1
- 102100002890 MCM2 Human genes 0.000 claims 1
- 101700084024 MCM7 Proteins 0.000 claims 1
Claims (4)
- 生体試料中の前立腺がん細胞を検出するための診断または予後判定のアッセイであって、
i)前記生体試料から単離細胞試料を得て、単離細胞試料を、少なくとも1つのMCMポリペプチドを特異的に結合する結合剤と接触させるステップと、
ii)単離細胞試料を、アンドロゲン受容体ポリペプチド(AR)を特異的に結合するさらなる結合剤と接触させるステップと、場合によって、
iii)単離細胞試料を、前立腺特異抗原(PSA)を特異的に結合するさらなる結合剤と接触させるステップと、
iv)2つ以上の結合剤の結合を検出するステップと、
v)前記単離細胞試料中の、2つ以上の結合剤を陽性に結合する細胞の数を決定するステップであって、陽性細胞の数が、生体試料中の前立腺がん細胞の存在の指標であるステップと
を含むアッセイ。 - 前記MCMポリペプチドが、MCM2である、請求項1に記載のアッセイ。
- 対象における前立腺がんを決定するための診断または予後判定アッセイであって、
i)第1単離生体試料を得て、試料を、PSAを特異的に結合する結合剤と接触させるステップと、場合によって、
ii)前記試料中のPSAの存在を検出するステップと、
iii)対象から第2生体試料を得て、単離細胞試料を調製して、前記細胞試料を、少なくとも1つのMCMポリペプチドおよびアンドロゲン受容体ポリペプチドを特異的に結合する第1および第2の結合剤と接触させるステップと、
iv)前記MCMポリペプチドおよびアンドロゲン受容体の存在を検出するステップと、
v)前記単離細胞試料中の、MCMおよびアンドロゲン受容体の両方を陽性に結合する細胞の数を決定するステップであって、陽性細胞の数が、前立腺がん細胞の存在の指標であるステップと
を含むアッセイ。 - 第1、第2および第3の結合剤を含むキットであって、前記結合剤が、それぞれMCMポリペプチド、アンドロゲン受容体ポリペプチドおよび前立腺特異抗原ポリペプチドを結合するキット。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GBGB0921873.6A GB0921873D0 (en) | 2009-12-15 | 2009-12-15 | Assay |
GB0921873.6 | 2009-12-15 | ||
PCT/GB2010/002274 WO2011073619A1 (en) | 2009-12-15 | 2010-12-15 | Assay |
Publications (2)
Publication Number | Publication Date |
---|---|
JP2013513812A JP2013513812A (ja) | 2013-04-22 |
JP2013513812A5 true JP2013513812A5 (ja) | 2014-02-06 |
Family
ID=41667106
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2012543890A Pending JP2013513812A (ja) | 2009-12-15 | 2010-12-15 | アッセイ |
Country Status (7)
Country | Link |
---|---|
US (2) | US20130171662A1 (ja) |
EP (2) | EP2977762A3 (ja) |
JP (1) | JP2013513812A (ja) |
CN (1) | CN102782497A (ja) |
CA (1) | CA2807870A1 (ja) |
GB (1) | GB0921873D0 (ja) |
WO (1) | WO2011073619A1 (ja) |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US11519916B2 (en) | 2015-06-08 | 2022-12-06 | Arquer Diagnostics Limited | Methods for analysing a urine sample |
ES2911415T3 (es) | 2015-06-08 | 2022-05-19 | Arquer Diagnostics Ltd | Métodos y kits |
GB201820868D0 (en) * | 2018-12-20 | 2019-02-06 | Arquer Diagnostics Ltd | Detection method |
Family Cites Families (14)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6307030B1 (en) * | 1988-04-15 | 2001-10-23 | The University Of North Carolina At Chapel Hill | Androgen receptor proteins, recombinant DNA molecules coding for such, and use of such compositions |
GB9704444D0 (en) * | 1997-03-04 | 1997-04-23 | Isis Innovation | Non-invasive prenatal diagnosis |
HUP0004134A2 (en) | 1997-10-21 | 2001-03-28 | Cancer Res Campaign Tech | Determination of cellular growth abnormality |
US6303323B1 (en) * | 1997-10-21 | 2001-10-16 | Cancer Research Campaign Technology Limited | Detection of dysplastic or neoplastic cells using anti-MCM5 antibodies |
US6355623B2 (en) * | 1998-09-24 | 2002-03-12 | Hopital-Sainte-Justine | Method of treating IBD/Crohn's disease and related conditions wherein drug metabolite levels in host blood cells determine subsequent dosage |
US6566130B1 (en) * | 2000-01-28 | 2003-05-20 | Henry M. Jackson Foundation For The Advancement Of Military Medicine | Androgen-regulated gene expressed in prostate tissue |
CA2427858A1 (en) * | 2000-11-03 | 2002-05-10 | University Of Vermont And State Agricultural College | Compositions for inhibiting grb7 |
US20070105105A1 (en) * | 2003-05-23 | 2007-05-10 | Mount Sinai School Of Medicine Of New York University | Surrogate cell gene expression signatures for evaluating the physical state of a subject |
US7666595B2 (en) * | 2005-02-25 | 2010-02-23 | The Brigham And Women's Hospital, Inc. | Biomarkers for predicting prostate cancer progression |
US20060211059A1 (en) * | 2005-03-18 | 2006-09-21 | Taneja Samir S | Methods of improving screening, diagnosis and staging of prostate cancer using serum testosterone |
CN101239181A (zh) * | 2007-02-08 | 2008-08-13 | 中国科学院上海生命科学研究院 | 一种和癌症有关的组蛋白去甲基化酶及其应用 |
GB0720113D0 (en) * | 2007-10-15 | 2007-11-28 | Cambridge Cancer Diagnostics L | Diagnostic, prognostic and predictive testing for cancer |
GB0800311D0 (en) | 2008-01-09 | 2008-02-20 | Cytosystems Ltd | Apparatus and method for filtering biological material |
GB0811567D0 (en) * | 2008-06-24 | 2008-07-30 | Cytosystems Ltd | Assay |
-
2009
- 2009-12-15 GB GBGB0921873.6A patent/GB0921873D0/en not_active Ceased
-
2010
- 2010-12-15 CA CA2807870A patent/CA2807870A1/en not_active Abandoned
- 2010-12-15 EP EP15185015.3A patent/EP2977762A3/en not_active Withdrawn
- 2010-12-15 CN CN2010800568880A patent/CN102782497A/zh active Pending
- 2010-12-15 WO PCT/GB2010/002274 patent/WO2011073619A1/en active Application Filing
- 2010-12-15 US US13/512,823 patent/US20130171662A1/en not_active Abandoned
- 2010-12-15 EP EP10801208A patent/EP2513651A1/en not_active Ceased
- 2010-12-15 JP JP2012543890A patent/JP2013513812A/ja active Pending
-
2014
- 2014-09-17 US US14/489,207 patent/US10267801B2/en active Active
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