JP2013510569A - 幹細胞の分離方法 - Google Patents
幹細胞の分離方法 Download PDFInfo
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- JP2013510569A JP2013510569A JP2012538741A JP2012538741A JP2013510569A JP 2013510569 A JP2013510569 A JP 2013510569A JP 2012538741 A JP2012538741 A JP 2012538741A JP 2012538741 A JP2012538741 A JP 2012538741A JP 2013510569 A JP2013510569 A JP 2013510569A
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M45/00—Means for pre-treatment of biological substances
- C12M45/04—Phase separators; Separation of non fermentable material; Fractionation
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M45/00—Means for pre-treatment of biological substances
- C12M45/09—Means for pre-treatment of biological substances by enzymatic treatment
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/04—Cell isolation or sorting
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Abstract
Description
130 ピストン
131 貫通孔
150 連結管
151 先端部
153 末端部
170 チューブ
190 弁
Claims (7)
- 幹細胞の分離方法において、
吸引物質が含まれた容器の内径と一致するピストンに1以上の貫通孔が形成され、前記貫通孔を通じて酵素や洗浄液が投入されるように連結管を形成し、前記連結管は、先端部が前記貫通孔と連結され、末端部が外部管や酵素や洗浄液などが含まれた他の容器に連結されており、
前記酵素や洗浄液を吸引物質が含まれた容器に投入するために、前記ピストンを引いて容器内に陰圧を形成させて、前記連結管とピストンの貫通孔を通じて酵素や洗浄液が前記容器内に投入されることを特徴とする幹細胞の分離方法。 - 幹細胞の分離方法において、
吸引物質が含まれた容器の内径と一致するピストンに1以上の貫通孔が形成され、前記貫通孔を通じて酵素や洗浄液が投入されるように連結管を形成し、前記連結管は、先端部が前記貫通孔と連結され、末端部が外部管や酵素や洗浄液などが含まれた他の容器に連結されており、
前記容器のピストンを押して容器内に陽圧が形成されると、ピストン部分の上昇流体が貫通孔と連結管を通じて外部に流出されることを特徴とする幹細胞の分離方法。 - 前記洗浄液などが投入された吸引物質を遠心分離などの過程を経て幹細胞のような有効成分が分離した後、前記容器のピストンを押して容器内に陽圧が形成されると、ピストン部分の上昇流体が貫通孔と連結管を通じて外部に流出されることを特徴とする請求項1に記載の幹細胞の分離方法。
- 前記連結管はチューブがさらに連結されて他の容器と連結されることを特徴とする請求項1〜3のうちいずれか1項に記載の幹細胞の分離方法。
- 前記連結管又はチューブと他の容器が連結される区間に定量制御のための計量手段や1以上の容器と選択的に連結されるために弁がさらに形成されることを特徴とする請求項4に記載の幹細胞の分離方法。
- 前記弁は、弁膜型一方向弁又はルアーロック付き三方向弁であることを特徴とする請求項5に記載の幹細胞の分離方法。
- 前記三方向弁は、第1弁が連結管又はチューブと連結され、第2弁が洗浄液などが含まれた容器又は有効成分抽出容器に連結され、第3弁が除去成分抽出容器又は洗浄液などが含まれた容器に連結されることを特徴とする請求項6に記載の幹細胞の分離方法。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR10-2009-0111080 | 2009-11-17 | ||
KR20090111080 | 2009-11-17 | ||
PCT/KR2009/006890 WO2011062313A1 (ko) | 2009-11-17 | 2009-11-23 | 줄기세포 분리방법 |
Publications (2)
Publication Number | Publication Date |
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JP2013510569A true JP2013510569A (ja) | 2013-03-28 |
JP5668075B2 JP5668075B2 (ja) | 2015-02-12 |
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Application Number | Title | Priority Date | Filing Date |
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JP2012538741A Active JP5668075B2 (ja) | 2009-11-17 | 2009-11-23 | 幹細胞の分離方法 |
Country Status (5)
Country | Link |
---|---|
US (1) | US8673639B2 (ja) |
EP (1) | EP2503007A4 (ja) |
JP (1) | JP5668075B2 (ja) |
CN (1) | CN102741424A (ja) |
WO (1) | WO2011062313A1 (ja) |
Families Citing this family (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP2503007A4 (en) | 2009-11-17 | 2013-10-09 | Hee-Young Lee | METHOD FOR THE SEPARATION OF STEM CELLS |
DE102011080218B4 (de) * | 2010-10-20 | 2014-11-20 | Human Med Ag | Verfahren und Vorrichtung zum Separieren von adulten Stammzellen aus Fettgewebe |
WO2016094451A1 (en) * | 2014-12-08 | 2016-06-16 | Saint-Gobain Performance Plastics Corporation | Closed system for extracting isolated medium |
DE102015109148B3 (de) * | 2015-06-10 | 2016-05-04 | Human Med Ag | Vorrichtung zum Separieren von regenerativen und adulten Stammzellen |
WO2017079136A1 (en) * | 2015-11-02 | 2017-05-11 | Lifecell Corporation | Devices and methods for automated filling and dispensing of adipose tissue with control of shear |
WO2017195225A1 (en) | 2016-05-10 | 2017-11-16 | Promoitalia Group Spa | Method for extracting and separating stem cells derived from adipose tissue for aesthetic treatments |
CN106994396B (zh) * | 2017-05-23 | 2019-03-29 | 武汉朗克医疗器械有限公司 | Prp连续离心分离装置及分离方法 |
Citations (3)
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JP2006513016A (ja) * | 2003-01-25 | 2006-04-20 | リー,ヒ−ヨン | 密閉型脂肪移植システム |
JP2007289076A (ja) * | 2006-04-25 | 2007-11-08 | Kaneka Corp | 脂肪組織から幹細胞を採取するのに適した細胞分離装置、およびその方法 |
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US3596652A (en) * | 1968-10-14 | 1971-08-03 | Bio Science Labor | Fluid separatory device |
US4197735A (en) * | 1978-11-06 | 1980-04-15 | Chase Instruments Corporation | Blood sedimentation rate test means |
KR100473363B1 (ko) | 2000-10-28 | 2005-03-07 | 김용년 | 액체공급장치 |
AU2001222342A1 (en) | 2000-07-22 | 2002-02-18 | Yong-Nyun Kim | Liquid supply apparatus |
US6659995B1 (en) * | 2000-11-17 | 2003-12-09 | Syde A. Taheri | Autologous myocyte micro granual retrieval and implantation (AMMGRI) |
TWI226077B (en) | 2001-07-05 | 2005-01-01 | Tokyo Electron Ltd | Liquid processing apparatus and liquid processing method |
KR100472577B1 (ko) | 2001-11-02 | 2005-02-22 | 김용년 | 액체공급장치 |
AU2003249642A1 (en) * | 2002-05-24 | 2003-12-12 | Biomet Manufacturing Corp. | Apparatus and method for separating and concentrating fluids containing multiple components |
KR100516923B1 (ko) | 2003-10-10 | 2005-09-26 | 신봉재 | 히트펌프 |
CN1871343B (zh) | 2003-11-04 | 2013-06-26 | 株式会社宝玛斯特 | 从脂肪组织制备干细胞的方法和系统 |
KR101006447B1 (ko) | 2003-12-18 | 2011-01-06 | 삼성전자주식회사 | 액정 표시 장치 및 그 구동 방법 |
US7588732B2 (en) * | 2004-03-30 | 2009-09-15 | Genesis Biosystems, Inc. | Autologus tissue harvesting and irrigation device |
KR100674927B1 (ko) | 2004-12-09 | 2007-01-26 | 삼성전자주식회사 | Cmp용 슬러리 조성물 및 그 제조 방법과 이들을 이용한기판 연마 방법 |
EP1947170B1 (en) * | 2005-10-21 | 2018-08-08 | Kaneka Corporation | Stem cell separating material and method of separation |
WO2007145438A1 (en) | 2006-06-15 | 2007-12-21 | Rnl Bio Co., Ltd | Cosmetic or plastic composition comprising multipotent stem cells derived from human adipose tissue, fibroblast, and adipose or adiopocyte |
US8968272B2 (en) * | 2006-10-06 | 2015-03-03 | Lipocosm Llc | Closed system and method for atraumatic, low pressure, continuous harvesting, processing, and grafting of lipoaspirate |
KR101013927B1 (ko) | 2008-06-12 | 2011-02-14 | 이희영 | 줄기세포 분리방법 |
EP2503007A4 (en) | 2009-11-17 | 2013-10-09 | Hee-Young Lee | METHOD FOR THE SEPARATION OF STEM CELLS |
-
2009
- 2009-11-23 EP EP09851498.7A patent/EP2503007A4/en not_active Withdrawn
- 2009-11-23 CN CN200980162506XA patent/CN102741424A/zh active Pending
- 2009-11-23 JP JP2012538741A patent/JP5668075B2/ja active Active
- 2009-11-23 WO PCT/KR2009/006890 patent/WO2011062313A1/ko active Application Filing
- 2009-11-23 US US13/509,757 patent/US8673639B2/en active Active
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
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JP2006513016A (ja) * | 2003-01-25 | 2006-04-20 | リー,ヒ−ヨン | 密閉型脂肪移植システム |
JP2007536941A (ja) * | 2003-06-18 | 2007-12-20 | サイトリ セラピューティクス インコーポレイテッド | オートロガス脂肪導入増進における、脂肪組織由来細胞の使用方法 |
JP2007289076A (ja) * | 2006-04-25 | 2007-11-08 | Kaneka Corp | 脂肪組織から幹細胞を採取するのに適した細胞分離装置、およびその方法 |
Also Published As
Publication number | Publication date |
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CN102741424A (zh) | 2012-10-17 |
US20120276629A1 (en) | 2012-11-01 |
US8673639B2 (en) | 2014-03-18 |
WO2011062313A1 (ko) | 2011-05-26 |
EP2503007A4 (en) | 2013-10-09 |
EP2503007A1 (en) | 2012-09-26 |
JP5668075B2 (ja) | 2015-02-12 |
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