JP2013506416A5 - - Google Patents
Download PDFInfo
- Publication number
- JP2013506416A5 JP2013506416A5 JP2012532045A JP2012532045A JP2013506416A5 JP 2013506416 A5 JP2013506416 A5 JP 2013506416A5 JP 2012532045 A JP2012532045 A JP 2012532045A JP 2012532045 A JP2012532045 A JP 2012532045A JP 2013506416 A5 JP2013506416 A5 JP 2013506416A5
- Authority
- JP
- Japan
- Prior art keywords
- cultured cells
- cell culture
- free solution
- culture support
- cells
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Ceased
Links
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 claims 12
- 238000000034 method Methods 0.000 claims 12
- 210000004748 cultured cell Anatomy 0.000 claims 5
- 238000004113 cell culture Methods 0.000 claims 4
- 239000011248 coating agent Substances 0.000 claims 4
- 238000000576 coating method Methods 0.000 claims 4
- 229920001577 copolymer Polymers 0.000 claims 2
- 229920001519 homopolymer Polymers 0.000 claims 2
- 229920000867 polyelectrolyte Polymers 0.000 claims 2
- 229920000642 polymer Polymers 0.000 claims 2
- 210000002536 stromal cell Anatomy 0.000 claims 2
- 210000004027 cell Anatomy 0.000 claims 1
- 238000011534 incubation Methods 0.000 claims 1
- 239000000758 substrate Substances 0.000 claims 1
Claims (11)
- 細胞を遊離させる方法であって、
a)基体上に固定化された多層高分子電解質コーティングを含む細胞培養支持体上に培養細胞を用意する段階、及び
b)DMSOを含む遊離溶液を添加することで細胞培養支持体から培養細胞を遊離させる段階
を含んでなる方法。 - 培養細胞が間葉間質細胞からなる、請求項1記載の方法。
- 培養細胞がヒト間葉間質細胞からなる、請求項1記載の方法。
- 遊離溶液中のDMSO濃度が約0.01〜1.0%の範囲内にある、請求項1又は請求項2記載の方法。
- 遊離溶液中のDMSO濃度が約0.02〜0.5%の範囲内にある、請求項4記載の方法。
- 遊離溶液中のDMSO濃度が約0.1〜0.5%の範囲内にある、請求項5記載の方法。
- 細胞培養支持体を室温でインキュベートすることで培養細胞が細胞培養支持体から遊離される、請求項1乃至請求項6のいずれか1項記載の方法。
- インキュベーションが約10〜30分間実施される、請求項7記載の方法。
- 多層高分子電解質コーティングが1以上のホモポリマー層を含む、請求項1乃至請求項8のいずれか1項記載の方法。
- 多層ポリマーコーティングが1以上のコポリマー層を含む、請求項1乃至請求項9のいずれか1項記載の方法。
- 多層ポリマーコーティングが1以上のホモポリマー層及び1以上のコポリマー層を含む、請求項1乃至請求項10のいずれか1項記載の方法。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US12/570,032 US8399252B2 (en) | 2009-09-30 | 2009-09-30 | Methods and kits for cell release |
US12/570,032 | 2009-09-30 | ||
PCT/SE2010/051033 WO2011040867A1 (en) | 2009-09-30 | 2010-09-27 | Methods and kits for cell release |
Publications (2)
Publication Number | Publication Date |
---|---|
JP2013506416A JP2013506416A (ja) | 2013-02-28 |
JP2013506416A5 true JP2013506416A5 (ja) | 2013-11-14 |
Family
ID=43780827
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2012532045A Ceased JP2013506416A (ja) | 2009-09-30 | 2010-09-27 | 細胞遊離のための方法及びキット |
Country Status (7)
Country | Link |
---|---|
US (2) | US8399252B2 (ja) |
EP (1) | EP2483391A4 (ja) |
JP (1) | JP2013506416A (ja) |
CN (1) | CN102575226A (ja) |
CA (1) | CA2772248A1 (ja) |
IN (1) | IN2012DN01891A (ja) |
WO (1) | WO2011040867A1 (ja) |
Families Citing this family (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US9534206B2 (en) | 2010-12-16 | 2017-01-03 | General Electric Company | Cell carrier, associated methods for making cell carrier and culturing cells using the same |
US9453196B2 (en) | 2010-12-16 | 2016-09-27 | General Electric Company | Cell carrier, methods of making and use |
US9926523B2 (en) | 2010-12-16 | 2018-03-27 | General Electric Company | Cell carriers and methods for culturing cells |
US9518249B2 (en) | 2010-12-16 | 2016-12-13 | General Electric Company | Cell carrier, associated methods for making cell carrier and culturing cells using the same |
US9453197B2 (en) | 2010-12-16 | 2016-09-27 | General Electric Company | Methods of making cell carrier |
BR112016006705A2 (pt) | 2013-09-27 | 2017-08-01 | 3M Innovative Properties Co | artigos de filme estruturados de dupla face |
CA3203767A1 (en) * | 2020-12-31 | 2022-07-07 | Ying-Chih Chang | Cell culture systems, methods and uses thereof |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7022522B2 (en) | 1998-11-13 | 2006-04-04 | Limin Guan | Macroporous polymer scaffold containing calcium phosphate particles |
JP4161106B2 (ja) | 2004-09-30 | 2008-10-08 | 独立行政法人科学技術振興機構 | 細胞剥離剤及び細胞シート剥離方法 |
ITRM20060020A1 (it) | 2006-01-18 | 2007-07-19 | Andrea Masotti | Metodo per la coltura di cellule |
GB2425074A (en) * | 2006-03-23 | 2006-10-18 | Ilika Technologies Ltd | Selective cellular adhesion on polymer microarrays |
US20080241926A1 (en) | 2007-03-02 | 2008-10-02 | Ilsoon Lee | Cell adhesion on surfaces of varying topographies |
-
2009
- 2009-09-30 US US12/570,032 patent/US8399252B2/en active Active
-
2010
- 2010-09-27 EP EP10820906.5A patent/EP2483391A4/en not_active Withdrawn
- 2010-09-27 JP JP2012532045A patent/JP2013506416A/ja not_active Ceased
- 2010-09-27 CN CN2010800450145A patent/CN102575226A/zh active Pending
- 2010-09-27 IN IN1891DEN2012 patent/IN2012DN01891A/en unknown
- 2010-09-27 WO PCT/SE2010/051033 patent/WO2011040867A1/en active Application Filing
- 2010-09-27 CA CA2772248A patent/CA2772248A1/en not_active Abandoned
-
2013
- 2013-02-25 US US13/776,053 patent/US8993322B2/en active Active
Similar Documents
Publication | Publication Date | Title |
---|---|---|
JP2013506416A5 (ja) | ||
JP2012105678A5 (ja) | ||
JP2014501518A5 (ja) | ||
JP2012210156A5 (ja) | ||
EA201270314A1 (ru) | Прямоосмотические мембраны | |
WO2012003479A3 (en) | Methods for culturing undifferentiated cells using sustained release compositions | |
WO2010048417A3 (en) | Perfusion bioreactors, cell culture systems, and methods for production of cells and cell-derived products | |
MX2014005952A (es) | Novedosos modulos solares, pilas de capas de soporte y metodos de fabricacion de los mismos. | |
GB201211309D0 (en) | Process for preparing membranes | |
BR112012025172A2 (pt) | células de ilhotas gabaritadas e agrupamentos de células de ilhotas pequenas para tratamento de diabetes. | |
JP2012522511A5 (ja) | ||
JP2016501013A5 (ja) | ||
JP2013529123A5 (ja) | ||
JP2016539641A5 (ja) | ||
NZ609731A (en) | Compositions of adult organ stem cells and uses thereof | |
BR112012028786A2 (pt) | biorreator para cultura de células e portador para um biorreator | |
WO2011133902A3 (en) | Cell culture medium comprising small peptides | |
WO2011150055A3 (en) | Methods, substrates, and systems useful for cell seeding of medical grafts | |
IN2012DN01268A (ja) | ||
IN2012DN01891A (ja) | ||
SG11201900715QA (en) | Method for culturing cell, and method for removing and killing cell from cell suspension | |
EA201170024A1 (ru) | Способы получения высокой плотности жизнеспособных клеток в культуре клеток млекопитающих | |
WO2011116922A3 (de) | Thermoresponsives substrat mit mikrogelen, verfahren zu dessen herstellung und kultivierungsverfahren für biologische zellen | |
MX343113B (es) | Medios de cultivo de celulas granulados en seco. | |
FR2960340B1 (fr) | Procede de realisation d'un support de substrat |