JP2013505719A5 - - Google Patents
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- Publication number
- JP2013505719A5 JP2013505719A5 JP2012530881A JP2012530881A JP2013505719A5 JP 2013505719 A5 JP2013505719 A5 JP 2013505719A5 JP 2012530881 A JP2012530881 A JP 2012530881A JP 2012530881 A JP2012530881 A JP 2012530881A JP 2013505719 A5 JP2013505719 A5 JP 2013505719A5
- Authority
- JP
- Japan
- Prior art keywords
- nucleic acid
- anion exchange
- acid
- anionic
- group
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 claims description 46
- 238000005349 anion exchange Methods 0.000 claims description 42
- 150000001449 anionic compounds Chemical class 0.000 claims description 37
- 150000007523 nucleic acids Chemical class 0.000 claims description 36
- 102000039446 nucleic acids Human genes 0.000 claims description 36
- 108020004707 nucleic acids Proteins 0.000 claims description 36
- 239000000463 material Substances 0.000 claims description 22
- 125000000129 anionic group Chemical group 0.000 claims description 19
- 150000003839 salts Chemical class 0.000 claims description 12
- 150000001735 carboxylic acids Chemical class 0.000 claims description 10
- 102000004190 Enzymes Human genes 0.000 claims description 8
- 108090000790 Enzymes Proteins 0.000 claims description 8
- 238000011529 RT qPCR Methods 0.000 claims description 8
- 150000001412 amines Chemical class 0.000 claims description 8
- 230000003321 amplification Effects 0.000 claims description 8
- 230000000694 effects Effects 0.000 claims description 8
- 238000010828 elution Methods 0.000 claims description 8
- 238000011880 melting curve analysis Methods 0.000 claims description 8
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 8
- MUBZPKHOEPUJKR-UHFFFAOYSA-N Oxalic acid Chemical compound OC(=O)C(O)=O MUBZPKHOEPUJKR-UHFFFAOYSA-N 0.000 claims description 6
- 230000002378 acidificating effect Effects 0.000 claims description 6
- 150000001413 amino acids Chemical class 0.000 claims description 6
- 238000006243 chemical reaction Methods 0.000 claims description 6
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 6
- LSNNMFCWUKXFEE-UHFFFAOYSA-M Bisulfite Chemical compound OS([O-])=O LSNNMFCWUKXFEE-UHFFFAOYSA-M 0.000 claims description 4
- BVKZGUZCCUSVTD-UHFFFAOYSA-L Carbonate Chemical compound [O-]C([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-L 0.000 claims description 4
- 102100034343 Integrase Human genes 0.000 claims description 4
- 229910019142 PO4 Inorganic materials 0.000 claims description 4
- ABLZXFCXXLZCGV-UHFFFAOYSA-N Phosphorous acid Chemical compound OP(O)=O ABLZXFCXXLZCGV-UHFFFAOYSA-N 0.000 claims description 4
- 229920002845 Poly(methacrylic acid) Polymers 0.000 claims description 4
- 229920002873 Polyethylenimine Polymers 0.000 claims description 4
- 108010020346 Polyglutamic Acid Proteins 0.000 claims description 4
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 claims description 4
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 4
- 238000004458 analytical method Methods 0.000 claims description 4
- 229920001577 copolymer Polymers 0.000 claims description 4
- 229960000633 dextran sulfate Drugs 0.000 claims description 4
- 238000006911 enzymatic reaction Methods 0.000 claims description 4
- YDSWCNNOKPMOTP-UHFFFAOYSA-N mellitic acid Chemical compound OC(=O)C1=C(C(O)=O)C(C(O)=O)=C(C(O)=O)C(C(O)=O)=C1C(O)=O YDSWCNNOKPMOTP-UHFFFAOYSA-N 0.000 claims description 4
- 239000000178 monomer Substances 0.000 claims description 4
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims description 4
- 239000010452 phosphate Substances 0.000 claims description 4
- 229920002643 polyglutamic acid Polymers 0.000 claims description 4
- 229920000642 polymer Polymers 0.000 claims description 4
- CYIDZMCFTVVTJO-UHFFFAOYSA-N pyromellitic acid Chemical compound OC(=O)C1=CC(C(O)=O)=C(C(O)=O)C=C1C(O)=O CYIDZMCFTVVTJO-UHFFFAOYSA-N 0.000 claims description 4
- 238000003757 reverse transcription PCR Methods 0.000 claims description 4
- 239000000523 sample Substances 0.000 claims description 4
- -1 organophosphates Chemical class 0.000 claims description 3
- 108060004795 Methyltransferase Proteins 0.000 claims description 2
- 239000004793 Polystyrene Substances 0.000 claims description 2
- 229920002125 Sokalan® Polymers 0.000 claims description 2
- 125000003342 alkenyl group Chemical group 0.000 claims description 2
- 125000003118 aryl group Chemical group 0.000 claims description 2
- 239000011324 bead Substances 0.000 claims description 2
- 239000012472 biological sample Substances 0.000 claims description 2
- 125000004432 carbon atom Chemical group C* 0.000 claims description 2
- 150000004649 carbonic acid derivatives Chemical class 0.000 claims description 2
- 239000012149 elution buffer Substances 0.000 claims description 2
- CTHCTLCNUREAJV-UHFFFAOYSA-N heptane-2,4,6-trione Chemical compound CC(=O)CC(=O)CC(C)=O CTHCTLCNUREAJV-UHFFFAOYSA-N 0.000 claims description 2
- 125000005842 heteroatom Chemical group 0.000 claims description 2
- 230000005764 inhibitory process Effects 0.000 claims description 2
- 229910052757 nitrogen Inorganic materials 0.000 claims description 2
- 235000006408 oxalic acid Nutrition 0.000 claims description 2
- 229910052760 oxygen Inorganic materials 0.000 claims description 2
- 150000003009 phosphonic acids Chemical class 0.000 claims description 2
- 229910052698 phosphorus Inorganic materials 0.000 claims description 2
- 229920001184 polypeptide Polymers 0.000 claims description 2
- 229920002223 polystyrene Polymers 0.000 claims description 2
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 2
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 2
- 238000000746 purification Methods 0.000 claims description 2
- 239000000377 silicon dioxide Substances 0.000 claims description 2
- 150000003460 sulfonic acids Chemical class 0.000 claims description 2
- 229910052717 sulfur Inorganic materials 0.000 claims description 2
- 150000001875 compounds Chemical class 0.000 description 4
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US23137109P | 2009-09-24 | 2009-09-24 | |
| US61/231,371 | 2009-09-24 | ||
| US29526910P | 2010-01-15 | 2010-01-15 | |
| US61/295,269 | 2010-01-15 | ||
| PCT/US2010/044586 WO2011037692A1 (en) | 2009-09-24 | 2010-08-05 | Compositions, methods, and kits for isolating and analyzing nucleic acids using an anion exchange material |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2013505719A JP2013505719A (ja) | 2013-02-21 |
| JP2013505719A5 true JP2013505719A5 (https=) | 2013-08-08 |
| JP5914338B2 JP5914338B2 (ja) | 2016-05-11 |
Family
ID=47890503
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2012530881A Expired - Fee Related JP5914338B2 (ja) | 2009-09-24 | 2010-08-05 | 陰イオン交換材料を使用した核酸の単離および分析のための組成物、方法およびキット |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP5914338B2 (https=) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR3031063B1 (fr) * | 2014-12-30 | 2017-02-10 | Biomerieux Sa | Complexe multicouches, procede de fabrication et utilisation du complexe |
| KR102488291B1 (ko) | 2015-03-20 | 2023-01-13 | 도레이 카부시키가이샤 | 핵산의 회수 방법 |
| EP3430135B1 (en) * | 2016-03-19 | 2026-02-11 | QIAGEN GmbH | Stabilization of rna |
| US20190203200A1 (en) | 2016-09-14 | 2019-07-04 | Toray Industries, Inc. | Method of collecting cell-free dna |
| GB201718803D0 (en) * | 2017-11-14 | 2017-12-27 | Phoenix Molecular Pte Ltd | Method for isolation of nucleic acids |
| KR20260047321A (ko) * | 2023-08-08 | 2026-04-07 | 라이프 테크놀로지스 코포레이션 | 핵산을 위한 샘플 제조 |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IL101356A (en) * | 1991-04-03 | 1996-08-04 | Perkin Elmer Corp | Solvents for the separation of nucleic acids by replacing anions |
| CN1643145A (zh) * | 2002-03-21 | 2005-07-20 | 艾夫西亚生物科技公司 | 寡核苷酸及其类似物的纯化方法 |
| JP2006280277A (ja) * | 2005-03-31 | 2006-10-19 | Tokyo Univ Of Agriculture & Technology | 核酸の抽出方法 |
| EP1882739A1 (en) * | 2006-06-30 | 2008-01-30 | Qiagen GmbH | Nucleic acid extraction method |
-
2010
- 2010-08-05 JP JP2012530881A patent/JP5914338B2/ja not_active Expired - Fee Related
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