JP2013213017A - Peracetic acid-including microbicidal composition - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a peracetic acid-including microbicidal composition effective to destructive funguses such as Paenibacillus bacteria and Bacillus cereus forming spores and having strong resistance to a peracetic acid/hydrogen peroxide formulation.SOLUTION: It is known that, regarding Paenibacillus bacteria and Bacillus cereus as spore forming bacteria which are high in chemical resistance, sterilization is difficult even if the conventional peracetic acid formulation is used. Regarding Bacillus cereus and Paenibacillus bacteria as spore forming bacteria, It has been found that a peracetic acid composition, in which the including ratio of hydrogen peroxide in a peracetic acid equilibrium composition is made lower, exhibits a high sterilization effect by utilizing a hydrogen peroxide content decomposition enzyme, and even in treatment for a short time, an effective peracetic acid-including microbicidal composition has been completed.

Description

The present invention relates to a peracetic acid sterilizing composition widely used for the purpose of sterilizing / cleaning manufacturing apparatuses, containers, and packaging materials in many fields such as food, brewing, and medical industries. More specifically, the present invention relates to a peracetic acid sterilizing composition used in various beverage foods and medical devices.

Peracetic acid preparations have strong bactericidal activity even at low concentrations, have immediate effects, have a wide antibacterial spectrum, and have excellent effects on bacterial spores, molds, yeasts, etc. Widely used in. However, even when a conventional peracetic acid preparation is used, it is known that sterilization is difficult for Bacillus cereus, which is a highly chemical-resistant spore-forming bacterium.

For this Bacillus cereus, the peracetic acid composition in which the peracetic acid concentration in the peracetic acid composition is 500 to 3000 ppm and the hydrogen peroxide / peracetic acid ratio is kept low to 0.7 or less has effective bactericidal properties. It is disclosed to show. (Patent Document 1 and Non-Patent Document 1) In Patent Document 1, a general peracetic acid-based disinfectant containing 10% peracetic acid and 15% hydrogen peroxide (thus, the hydrogen peroxide / peracetic acid ratio is 1.5). It is described that a peracetic acid concentration of 500 to 3000 ppm is prepared by diluting with water. The document does not describe any method for reducing the hydrogen peroxide / peracetic acid ratio of the preparation solution to 0.7 or less, but in Example 1, hydrogen peroxide is further added to the preparation solution to obtain a desired solution. It is described that a sample of hydrogen peroxide / peracetic acid ratio is prepared.
However, in the above-described method of diluting by adding water to the peracetic acid composition, the ratio of peracetic acid to hydrogen peroxide does not change, and the hydrogen peroxide / peracetic acid ratio remains at 1.5. In addition, the method of adding hydrogen peroxide further increases the hydrogen peroxide concentration. In any case, the peracetic acid concentration is set to 500 to 3000 ppm, and the hydrogen peroxide / peracetic acid ratio is suppressed to 0.7 or less. A peracetic acid composition cannot be obtained.
In recent years, regarding various beverage foods, problems of resistant bacteria such as Paenibacillus genus bacteria exhibiting strong resistance to the peracetic acid preparation have become apparent. (Patent Document 2)

Further, in the peracetic acid composition, the amount of hydrogen peroxide is reduced by using a catalase enzyme to reduce the hydrogen peroxide and peracid accumulated in the composition when the food package is sterilized. Methods have been reported to save costs such as replacement, labor, and labor. However, in this report, the amount of catalase enzyme used is defined by a simple weight concentration, and is not represented by a titer that quantitatively represents the resolution of hydrogen peroxide. General enzymes such as catalase contain impurities, etc. for purification from the living body, and are deactivated over time, so even if the amount used is specified other than the titer that directly represents the activity, the effect is definitely obtained It ’s difficult.

WO2007 / 148410 JP 2009-113858 A Special table 2011-517946 gazette

Antibacterial and fungi July 2006, pages 397-400

The present invention provides a peracetic acid-containing bactericidal composition that exhibits a high bactericidal effect in a short time against spore-forming bacteria such as Bacillus cereus and Paenibacillus spp.

The present inventors have demonstrated that a peracetic acid composition having a low hydrogen peroxide content in a peracetic acid equilibrium composition has a high bactericidal effect on Bacillus cereus and Paenibacillus spp. Found to show. Therefore, as a result of intensive studies, a peracetic acid-containing sterilizing composition effective for Bacillus cereus and Paenibacillus bacteria of the present invention is obtained by efficiently increasing the peracetic acid / hydrogen peroxide ratio using a hydrogen peroxide-degrading enzyme. Completed.

That is, the present invention has the following configuration.

Contains peracetic acid / hydrogen peroxide / acetic acid and hydrogen peroxide-degrading enzyme, and has a peracetic acid concentration of 1500 ppm or more and a peracetic acid / hydrogen peroxide ratio of 7 or more, or a peracetic acid concentration of 1900 ppm or more and peracetic acid / peroxidation. Paenibacillus having a hydrogen ratio of 4 or more, a peracetic acid concentration of 2500 ppm or more and a peracetic acid / hydrogen peroxide ratio of 3 or more, or a peracetic acid concentration of 3200 ppm or more and a peracetic acid / hydrogen peroxide ratio of 2 or more. Peracetic acid-containing bactericidal composition against bacteria and Bacillus cereus.

0.5U. The peracetic acid-containing bactericidal composition against Paenibacillus bacteria and Bacillus cereus according to paragraph [0010], comprising hydrogen peroxide-degrading enzyme of T / ml or more.

7.5U. The peracetic acid-containing bactericidal composition against Paenibacillus bacteria and Bacillus cereus according to paragraph [0011], comprising hydrogen peroxide-degrading enzyme of T / ml or more.

1500 ppm or more peracetic acid and 0.5 U.S. A peracetic acid-containing disinfectant composition against Paenibacillus bacteria and Bacillus cereus, which contain T / ml or more hydrogen peroxide-degrading enzyme and do not contain hydrogen peroxide.

A method for sterilizing Paenibacillus bacteria and Bacillus cereus using the peracetic acid-containing sterilizing composition according to any one of paragraphs [0010] to [0013].

The sterilization method according to paragraph [0014], wherein the sterilization treatment is performed at 60 ° C to 95 ° C.

The sterilization method according to paragraph [0014], wherein the sterilization treatment is performed in 5 seconds to 60 seconds.

Peracetic acid hydrogen peroxide mixture formed by equilibrium reaction of hydrogen peroxide and acetic acid was charged with 0.5 U. A method for producing a peracetic acid-containing bactericidal composition for Paenibacillus bacteria and Bacillus cereus, which is produced by adding hydrogen peroxide-degrading enzyme so as to be T / ml or more and decomposing hydrogen peroxide.

A mixture of peracetic acid and hydrogen peroxide formed by an equilibrium reaction of hydrogen peroxide and acetic acid was added to 7.5 U. A method for producing a peracetic acid-containing bactericidal composition for Paenibacillus bacteria and Bacillus cereus, which is produced by adding hydrogen peroxide-degrading enzyme so as to be T / ml or more and decomposing hydrogen peroxide.

The present invention will be described in detail below.
Normally, peracetic acid is produced by an equilibrium reaction between hydrogen peroxide and acetic acid. By reducing the hydrogen peroxide content ratio in the equilibrium composition, it is effective against spore-forming bacteria such as Bacillus cereus and Paenibacillus bacteria. Although it exhibits a bactericidal effect, it is not preferable to use an extreme ratio of hydrogen peroxide and acetic acid from the viewpoint of economy and safety. During production, trying to obtain a peracetic acid composition with a significantly high peracetic acid / hydrogen peroxide ratio by reducing the hydrogen peroxide ratio extremely results in an equilibrium composition with a high acetic acid concentration, increasing the risk. Therefore, it becomes difficult to handle. In order to avoid danger, if a low-concentration peracetic acid composition is diluted to reduce the concentration of peracetic acid, it is not suitable for practical use from the economical aspect of transportation and storage.

The method for producing a peracetic acid-containing sterilizing composition of the present invention comprises a general peracetic acid composition preparation step followed by a step of increasing the peracetic acid / hydrogen peroxide ratio by adding a hydrogen peroxide-degrading enzyme.
As a method for preparing the peracetic acid composition, an appropriate method may be selected from the viewpoints of safety, economy, speed of preparation time, and the like. Moreover, you may utilize a commercially available peracetic acid formulation.
Specifically, a method in which acetic acid and hydrogen peroxide are sufficiently mixed and allowed to stand for several days to several weeks under an appropriate temperature condition can be exemplified. This preparation process is an equilibrium reaction represented by ([peracetic acid] · [water]) / ([acetic acid] · [hydrogen peroxide]) = K (equilibrium constant) in concentration. By appropriately adjusting the amounts of hydrogen peroxide and hydrogen peroxide, a peracetic acid composition having a desired peracetic acid concentration can be obtained. When equilibrium is reached, it always contains peracetic acid, hydrogen peroxide, acetic acid, and water.
As a modification of this preparation method, there is a method of obtaining a peracetic acid composition from glycerin acetate and hydrogen peroxide.

The peracetic acid composition thus obtained is allowed to act on a hydrogen peroxide-degrading enzyme to decompose the hydrogen peroxide contained therein, thereby obtaining the peracetic acid-containing sterilizing composition of the present invention.
As the hydrogen peroxide-degrading enzyme used in the present invention, various catalases extracted from filamentous fungi can be used. However, the enzyme activity can be expressed and maintained in the peracetic acid composition, and the hydrogen peroxide is rapidly decomposed to a desired level. Those that can be reduced to are desirable. Specifically, Ask Super 25 (trade name, manufactured by Mitsubishi Gas Chemical Co., Ltd.) can be exemplified.

  It should be noted here that the peracetic acid composition is formed by the equilibrium of hydrogen peroxide / acetic acid / peracetic acid / water. Therefore, even in the peracetic acid-containing sterilizing composition of the present invention, even after hydrogen peroxide is reduced by the added hydrogen peroxide-degrading enzyme, or even during the reduction, peracetic acid gradually reacts with water over time. Then, it returns to hydrogen peroxide and acetic acid, and becomes a composition of hydrogen peroxide / acetic acid / peracetic acid / water in a concentration state defined by the equilibrium constant K. In the state defined by this equilibrium constant K, in most cases, the peracetic acid concentration and the peracetic acid / hydrogen peroxide ratio are no longer those of the present invention and are sufficient for Bacillus cereus and Paenibacillus bacteria. There is a high possibility of not having a bactericidal effect.

Inadvertently degrading hydrogen peroxide with a low-concentration hydrogen peroxide-degrading enzyme will take a long time to decompose, and before the desired peracetic acid / hydrogen peroxide ratio is reached, There is a possibility that the hydrogen peroxide-degrading enzyme is inactivated by the pH and the desired peracetic acid / hydrogen peroxide ratio cannot be reached.

  Therefore, in the present invention, it is desirable that hydrogen peroxide is decomposed as quickly as possible by the added hydrogen peroxide-degrading enzyme. To do so, consider the concentration of hydrogen peroxide to be decomposed and how much the perhydrolytic enzyme to be used shows in the presence of hydrogen peroxide / acetic acid / peracetic acid. It is preferable to determine the concentration of the added. Generally, the added concentration is 0.5U. T / ml or more is preferable, and more preferably 5U. T / ml or more, more preferably 7.5U. T / ml or more. In particular, it is preferable to add hydrogen peroxide-degrading enzyme so that the higher the concentration of hydrogen peroxide contained, the higher the concentration, in terms of preparation in a short time.

  In producing the peracetic acid-containing sterilizing composition of the present invention, after adding hydrogen peroxide-degrading enzyme to the peracetic acid composition to reduce the hydrogen peroxide, the peracetic acid concentration becomes a desired level within the scope of the present invention. The peracetic acid composition may be diluted to a desired peracetic acid concentration, and then hydrogen peroxide decomposing enzyme is added to reduce the hydrogen peroxide. An acetic acid / hydrogen peroxide ratio may be used.

  Furthermore, it can be used until the bactericidal effect on Bacillus cereus and Paenibacillus bacteria and the peracetic acid / hydrogen peroxide ratio reduced by the hydrogen peroxide-degrading enzyme returns due to the equilibrium reaction over time and falls outside the range of the present invention. From the standpoint of making the time as long as possible, the peracetic acid / hydrogen peroxide ratio in the present invention is preferably as high as possible. Specifically, when the peracetic acid concentration is 1500 ppm or more, the peracetic acid / hydrogen peroxide ratio is 7 or more, when the peracetic acid concentration is 1900 ppm or more, the peracetic acid / hydrogen peroxide ratio is 4 or more, and when the peracetic acid concentration is 2500 ppm or more, peracetic acid. When the / hydrogen peroxide ratio is 3 or more and the peracetic acid concentration is 3000 ppm or more, the peracetic acid / hydrogen peroxide ratio is 2 or more. Most preferred is a peracetic acid-containing sterilizing composition in which hydrogen peroxide is substantially completely decomposed from the viewpoint of sterilizing effect against Bacillus cereus and Paenibacillus bacteria.

  From the viewpoint of the present invention, the higher the peracetic acid concentration and the higher the peracetic acid / hydrogen peroxide ratio, the higher the bactericidal effect, but the peracetic acid concentration is 1500 ppm to 12000 ppm from the viewpoint of handling and economy. A more preferable concentration is 1900 ppm to 10000 ppm, a further preferable concentration is 2500 ppm to 10000 ppm, and a most preferable concentration is 3000 ppm to 8000 ppm.

The conditions for performing the sterilization treatment using the peracetic acid-containing sterilizing composition of the present invention include a longer time and a higher temperature as the time and temperature at which the peracetic acid-containing sterilizing composition acts on bacterial cells such as spores. preferable.
However, in consideration of practicality such as work efficiency and heat resistance of the container in sterilization treatment of food and beverage containers, the action time is preferably 5 seconds to 120 seconds, more preferably 5 seconds to 60 seconds, More preferably, it is 5 seconds to 15 seconds. Moreover, as temperature of a disinfection process, 60 to 95 degreeC is preferable, More preferably, it is 65 to 90 degreeC, More preferably, it is 65 to 75 degreeC.

Moreover, there is no restriction | limiting in particular in the usage form of the peracetic acid containing disinfection composition of this invention, What is necessary is just to take an optimal form according to the object used, apparatus installation, and the condition. For example, in the case of the sterilization treatment of the container, a method of immersing the target container in the present composition can be exemplified. Or the method of flowing this composition so that a container surface may be wash | cleaned can be illustrated. Moreover, the method of spraying a suitable quantity of this composition on the container surface can be illustrated. After the treatment, it is desirable to wash the object with clean aseptic water or the like so that the remaining composition is sufficiently removed. The composition after treatment can be reused as long as there is no particular hindrance, but attention should be paid to a decrease in the concentration of peracetic acid.

Moreover, you may add an additive etc. with respect to the peracetic acid containing disinfection composition of this invention according to the objective and use. However, the addition of metals and compounds that cause the decomposition of peracetic acid must be avoided. In the case of food and beverage, it is necessary to pay attention to whether or not the use of the additive is safe for the human body.
Examples of the additive include 1-hydroxyethylidene-1,1-diphosphonic acid, aminotrimethylenephosphonic acid, pyrophosphoric acid, 2,6-pyridinedicarboxylic acid and the like as stabilizers. As sterilization aids, benzyl alcohol, 2-phenoxyethanol, hexanol, heptanol, phenylethyl alcohol, chlorobutanol, propylene glycol, 1,5-pentanediol, hexylene glycol, p-ethylphenol, p-propylphenol, Examples include sucrose glycerin ester, sorbitol, glycerin triacetate, chloroacetic acid, formic acid, sodium formate. The cleaning aid is not particularly limited as long as it is a general surfactant. Depending on the purpose, one or more anionic, cationic or nonionic surfactants may be selected. Particularly preferred are sodium secondary alkyl sulfonate or alkylbenzene sulfonic acid and polyoxyethylene lauryl. Sodium ether sulfate (LES 70%) is blended at a ratio of 85:15.

  Although the detailed mechanism is unknown, both peracetic acid and hydrogen peroxide produce a bactericidal effect due to their oxidizing action, and the effect is exhibited by peracetic acid and hydrogen peroxide permeating the cell / spore membrane. It is considered to be. In the present invention, hydrogen peroxide acts as an inhibitory effect on the bactericidal effect of peracetic acid on Bacillus cereus and Paenibacillus spp., But the inhibitory action occurs antagonistically in the presence of co-existence. Yes, if the bacterial cells are pretreated with hydrogen peroxide, then the hydrogen peroxide is removed and treated again with peracetic acid alone, the bactericidal effect of peracetic acid is not inhibited. Therefore, for the purpose of the present invention, as described above, it is desirable that the hydrogen peroxide content is as close to zero as possible, and the lower the hydrogen peroxide content, the lower the concentration of peracetic acid. The sterilizing effect can be demonstrated. However, when sterilizing containers that have been contaminated by bacteria other than bacteria belonging to the genus Bacillus cereus or Paenibacillus, and if these bacteria are also targeted for sterilization, these bacteria are often more sensitive to hydrogen peroxide. There are many things, and there is a merit in terms of a total sterilization effect that some hydrogen peroxide is contained. Therefore, it is desirable to adjust the peracetic acid / hydrogen peroxide concentration ratio according to the purpose of use and the situation.

The peracetic acid-containing sterilizing composition according to the present invention can be used in fields and applications in which contamination and growth of fungi and microorganisms including Paenibacillus bacteria and Bacillus cereus are problematic due to its excellent sterilizing power. Aseptic packaging containers, equipment / containers, indoor environment sterilization, medical-related, high-level disinfection / endoscope / dialysis line / other medical equipment / in-hospital environment sterilization, linen-related, agricultural / fishery related equipment / equipment, pathogen sterilization Can be used.

The peracetic acid composition was prepared as follows.
A suitable amount of 35% hydrogen peroxide and glacial acetic acid or 80% acetic acid were mixed, and pyrophosphoric acid was further added to a concentration of 0.3%, followed by stirring to obtain a uniform solution. This was left to stand in a 40 ° C. warm bath for 3 days. Further, the mixture was allowed to stand at room temperature for 3 days to obtain a desired peracetic acid composition.
When 35% hydrogen peroxide: 52.3%, 80% acetic acid: 47.4%, and pyrophosphoric acid: 0.3%, the peracetic acid concentration was 12.1% and hydrogen peroxide was 12. 9%, acetic acid was 28%, and the peracetic acid / hydrogen peroxide concentration was 0.9. Also, by changing the ratio of hydrogen peroxide to acetic acid, 10% peracetic acid, 17% hydrogen peroxide, 21% acetic acid, 7.5% peracetic acid, 2.6% hydrogen peroxide, 58.1% acetic acid A peracetic acid composition was also prepared.
The peracetic acid composition thus prepared was appropriately diluted with water as necessary.

The concentration of peracetic acid was determined by the following method.
(1) Measurement of total peroxide concentration Approximately 0.1 ml of pure water was added to 0.1 g of a solution containing peracetic acid, and (1 + 9) sulfuric acid 10 ml, 1N potassium iodide solution 10 ml, 0.5% ammonium molybdate solution 1 to 2 drops. And titrated with N / 10 sodium thiosulfate standard solution.
(2) Measurement of hydrogen peroxide concentration Add about 20 ml of pure water to 0.1 g of a solution containing the same peracetic acid, add (1 + 9) 10 ml of sulfuric acid and 2-3 drops of ferroin indicator, and titrate with N / 10 cerium sulfate standard solution. did.
The peracetic acid concentration was obtained by converting a value obtained by subtracting (2) hydrogen peroxide concentration from (1) total peroxide concentration to peracetic acid according to the following formula.
Peracetic acid concentration (%) = (total peroxide concentration% −hydrogen peroxide concentration%) × 76/34

A commercially available catalase enzyme was used as the hydrogen peroxide decomposing enzyme, and hydrogen peroxide was decomposed by the following procedure.
Ask super 25 (trade name, manufactured by Mitsubishi Gas Chemical Co., Ltd.) was added to the prepared peracetic acid composition, and the hydrogen peroxide contained therein was decomposed by being left at 40 ° C. The concentration of catalase added was 0.13 U. T / ml to 2.6U. T / ml.

The titer of catalase can be determined as follows.
(1) 0.11 g of a 35% hydrogen peroxide solution is dissolved in 0.05M phosphate buffer (6.8 g of potassium dihydrogen phosphate (KH2PO4) and 8.71 g of dipotassium hydrogen phosphate (K2HPO4) in pure water. Prepared to 1000 ml), adjusted to 100 g to obtain a base solution, and heated to 30 ° C. in advance.
(2) 1 ml of a 10,000-fold dilution of catalase was placed in a 100 ml glass bottle and heated to 30 ° C. for 5 minutes. 5 ml of the base solution was added to 1 ml of the catalase diluted solution, and the reaction was performed accurately for 5 minutes.
(3) After 5 minutes, (1 + 9) 2 ml of sulfuric acid was added to stop the reaction.
(4) 1 ml of 1N potassium iodide and 1 drop of 1% ammonium molybdate solution were added, and titrated with N / 200 sodium thiosulfate solution using a starch indicator.
(5) As Blank, 1 ml of DMW was added to 5 ml of the base solution, and the operations of (3) and (4) were performed.
(6) The amount of enzyme capable of decomposing 1 μM H 2 O 2 per minute under the above conditions is 1 U. T.

For verification of the bactericidal effect, Bacillus subtilis (Bacillus subtilis), which is a bacterium belonging to the genus Bacillus cereus, Paenibacillus, and spore, was used.

As an index of the bactericidal effect of the peracetic acid-containing bactericidal composition of the present invention, C ₆₅ (Colony
Forming Unit) = 0.
Here, the D value (1D) means the initial number of microorganisms (initial number of spores) (N ₀ ) and the number of surviving bacteria (N ₀ ) when sterilization is performed with the composition under test at a constant temperature. ) (N / N ₀ ) is a processing time (minutes) required to be 1/10. In the present invention, the D value at 65 ° C. was used as an index of sterilizing power.

Further, regarding aseptic filling of non-heat-resistant PET bottles, the processing temperature, peracetic acid concentration, and processing time used in each bottling company are different, but in general, the processing temperature is 53 ° C to 65 ° C, and the processing time is 10 seconds to 20 seconds. Since it was performed in seconds, the required sterilization time was evaluated as being within 0.25 minutes (15 seconds).

(Sterilization test method)
(1) Into a 20 ml sterilized beaker, 9 ml of the test solution of peracetic acid composition was dispensed.
(2) Placed in a 65 ° C. warm bath and held for 5 minutes with stirring.
(3) 1 ml of spore solution was inoculated, 1 ml was collected after a predetermined time, and immediately mixed with 9 ml of ice-cooled inactivating agent.
(4) Serial dilution, pour on standard agar medium (manufactured by Nissui Pharmaceutical), and culture at 35 ° C. for 2 days.
(5) The number of colonies was counted to determine the number of surviving bacteria, and the D value was calculated.

Example 1
[Decomposition of hydrogen peroxide by catalase enzyme]
The results of changes over time in the amount of catalase added and the ratio of peracetic acid (PAA) / hydrogen peroxide (H ₂ O ₂ ) are shown below.

(Examples 2 to 16, Comparative Examples 1 to 10)
[Evaluation of sterilizing power]
Using Paenibacillus chibensis as a bacterium belonging to the genus Paenibacillus, the bactericidal properties of peracetic acid-containing bactericidal compositions having various peracetic acid / hydrogen peroxide ratios were examined.
The results are shown below.

  From the above results, the peracetic acid concentration of the present invention is 1500 ppm or more and the peracetic acid / hydrogen peroxide ratio is 7 or more, or the peracetic acid concentration is 1900 ppm or more and the peracetic acid / hydrogen peroxide ratio is 4 or more. A peracetic acid-containing sterilizing composition having an acetic acid concentration of 2500 ppm or more and a peracetic acid / hydrogen peroxide ratio of 3 or more, or a peracetic acid concentration of 3200 ppm or more and a peracetic acid / hydrogen peroxide ratio of 2 or more is Paenibacillus tibensis The necessary and sufficient bactericidal power was shown.

The peracetic acid composition according to the present invention is a food-related, aseptic packaging container, equipment / container, indoor environment sterilization, medical-related, high-level disinfection / endoscope / dialysis line / other medical equipment / in-hospital environment sterilization, linen It is an effective composition for sterilization of harmful bacteria including Paenibacillus spp. And Bacillus cereus in fields and uses such as related, agricultural / fishery related equipment and sterilizing pathogens.

Claims (9)

Peracetic acid concentration of 1500 ppm or more and peracetic acid / hydrogen peroxide ratio of 7 or more, or peracetic acid concentration of 1900 ppm or more and peracetic acid / hydrogen peroxide ratio of 4 or more, or peracetic acid concentration of 2500 ppm or more and peracetic acid Paenibacillus bacterium containing peracetic acid / hydrogen peroxide / acetic acid and hydrogen peroxide degrading enzyme having a peracetic acid / hydrogen peroxide ratio of 3 or more, or a peracetic acid concentration of 3200 ppm or more and a peracetic acid / hydrogen peroxide ratio of 2 or more And a bactericidal composition containing peracetic acid against Bacillus cereus. 0.5U. The peracetic acid-containing bactericidal composition for Paenibacillus bacteria and Bacillus cereus according to claim 1, comprising hydrogen peroxide-degrading enzyme of T / ml or more. 7.5U. The peracetic acid-containing bactericidal composition for Paenibacillus bacteria and Bacillus cereus according to claim 2, comprising hydrogen peroxide-degrading enzyme of T / ml or more. 1500 ppm or more peracetic acid and 0.5 U.S. A peracetic acid-containing disinfectant composition against Paenibacillus bacteria and Bacillus cereus, which contain T / ml or more hydrogen peroxide-degrading enzyme and do not contain hydrogen peroxide. The sterilization method with respect to the genus Paenibacillus and Bacillus cereus using the peracetic acid containing disinfection composition in any one of Claims 1-4. The sterilization method according to claim 5, wherein the sterilization treatment is performed at 60 ° C to 95 ° C. The sterilization method according to claim 5, wherein the sterilization treatment is performed in 5 seconds to 60 seconds. A peracetic acid hydrogen peroxide mixture formed by the equilibrium reaction of hydrogen peroxide and acetic acid is charged with 0.5 U. A method for producing a peracetic acid-containing bactericidal composition for Paenibacillus bacteria and Bacillus cereus, which is produced by adding hydrogen peroxide-degrading enzyme so as to be T / ml or more and decomposing hydrogen peroxide. Peracetic acid hydrogen peroxide mixture formed by equilibrium reaction of hydrogen peroxide and acetic acid was added to 7.5U. A method for producing a peracetic acid-containing bactericidal composition for Paenibacillus bacteria and Bacillus cereus, which is produced by adding hydrogen peroxide-degrading enzyme so as to be T / ml or more and decomposing hydrogen peroxide.