JP6371186B2 - Disinfectant composition - Google Patents

Description

  The present invention relates to a disinfectant composition containing peracetic acid, and more specifically, a disinfectant composition capable of controlling bacteria having resistance to peracetic acid and having reduced influence on equipment corrosion. It provides things.

  Peracetic acid is commercially available as an equilibrium mixed solution of peracetic acid / hydrogen peroxide / acetic acid / water, but shows a high sterilizing effect on a wide range of microorganisms in a low concentration and in a short time contact. Since it is water and water, it is highly safe, and in the food industry, it is used in large quantities for the purpose of sterilization of equipment, equipment, and packaging containers.

  Peracetic acid has excellent sterilizing properties against bacteria, fungi, viruses, etc., and has low sensitization and mutagenicity to humans, and its decomposition products are relatively safe compounds of acetic acid and hydrogen peroxide. It is. For this reason, peracetic acid-based disinfectants containing peracetic acid, hydrogen peroxide, and acetic acid are highly safe and are used in various cleaning agents and disinfectants. For the purpose of reinforcement, a surfactant such as a nonionic surfactant may be blended (see, for example, Patent Documents 1 to 3). In recent years, various foods and beverages have been exposed to the problem of resistant bacteria such as Paenibacillus spp. That exhibit strong resistance to peracetic acid. As a countermeasure, the concentration of residual hydrogen peroxide has been reduced. In addition, a method using a peracetic acid-based disinfectant having a high hydrogen peroxide concentration (Patent Document 4), a method for increasing the peracetic acid concentration (Patent Document 5), and the like have been proposed.

Japanese translation of PCT publication No. 8-502518 JP 2001-072996 A JP 2007-084589 A JP 2009-113858 A JP 2012-219053 A

  However, by reducing the concentration of hydrogen peroxide, the concentration of acetic acid increases, and there is a problem of affecting the packing of equipment, such as swelling and deterioration. There is a risk that the residual amount of hydrogen peroxide in the subsequent container becomes high, and there is a risk of mixing into the product. Furthermore, there is a problem that the method of increasing the peracetic acid concentration is expensive.

  Accordingly, the problem to be solved by the present invention is to provide a disinfectant composition excellent in disinfecting resistant bacteria such as Paenibacillus bacteria having strong resistance to peracetic acid and having low corrosiveness to stainless steel. Is to provide.

  In order to solve the above problems, the present inventors have intensively studied. As a result, a disinfectant composition containing a catalase enzyme together with peracetic acid, hydrogen peroxide, acetic acid and a chelating agent is a conventional peracetic acid disinfectant. The present inventors have found that the problems that the composition has can be solved, and have completed the present invention.

That is, the present invention
A disinfectant composition comprising (1) (1) (A) peracetic acid, (B) hydrogen peroxide, (C) acetic acid, (D) catalase enzyme, (E) chelating agent, and (F) water. And
(A) Peracetic acid concentration of 100 to 10000 ppm,
(D) 1-10 ppm of catalase enzyme,
(E) 1 to 1000 ppm of chelating agent,
And the mass ratio (A) / (B) of (A) peracetic acid and (B) hydrogen peroxide is 1 or more,
(D) The disinfectant composition, wherein the mass ratio (D) / (E) of the catalase enzyme and (E) chelating agent is 0.005 to 10,
( 2 ) The disinfectant composition according to (1) above, wherein the chelating agent of component (E) is an organic phosphonic acid and / or an organic phosphonate.
( 3 ) The chelating agent for component (E) is a compound selected from 1-hydroxyethylidene-1,1-diphosphonic acid, 2-phosphonobutane-1,2,4-tricarboxylic acid or salts thereof. The disinfectant composition according to the above (1) to (2) ,
( 4 ) The disinfectant composition according to any one of (1) to ( 3 ), further comprising (G) a nonionic surfactant and / or (H) an anionic surfactant in an amount of 10 to 50000 ppm. object,
( 5 ) The disinfectant composition according to any one of (1) to ( 4 ) above, wherein the disinfectant is treated by heating at a use temperature of 20 ° C to 70 ° C. Method,
(6) A sterilization method using the sterilization composition according to any one of (1) to ( 3 ) above ,
A first agent containing (A) peracetic acid, (B) hydrogen peroxide, (C) acetic acid, (E) chelating agent and (F) water, and a second agent containing (D) component immediately before use. Preparing the sterilizing composition by mixing, and subjecting to a sterilization operation,
(7) A concentrated composition containing at least one of (A) peracetic acid, (B) hydrogen peroxide, (C) acetic acid, and (E) a chelating agent, and (D) catalase enzyme and (F) water Add, dilute,
(A) Peracetic acid concentration of 100 to 10000 ppm,
(D) 1 to 10 ppm of catalase enzyme,
(E) 1 to 1000 ppm of chelating agent,
Contains and
(A) Mass ratio (A) / (B) of peracetic acid and (B) hydrogen peroxide is 1 or more,
(D) The method for producing a disinfectant composition, wherein the mass ratio (D) / (E) of catalase enzyme and (E) chelating agent is adjusted to 0.005 to 10,
Is a summary.

  The disinfectant composition of the present invention has excellent disinfecting properties against bacteria, fungi, viruses and the like, and also resistant bacteria such as Paenibacillus spp. Excellent sterilization effect and low corrosion resistance to stainless steel.

  The disinfectant composition of the present invention comprises (A) peracetic acid, (B) hydrogen peroxide, (C) acetic acid, (D) catalase enzyme, (E) chelating agent, (F) disinfectant containing water. A composition comprising (A) a concentration of peracetic acid of 100 to 10000 ppm, (D) a catalase enzyme of 0.01 to 250 ppm, (E) a chelating agent of 1 to 1000 ppm, and (A) peracetic acid and ( B) The mass ratio (A) / (B) of hydrogen peroxide is 1 or more, and the mass ratio (D) / (E) of (D) catalase enzyme and (E) chelating agent is 0.002-10. The disinfectant composition of the present invention comprises a concentrated composition containing at least one of (A) peracetic acid, (B) hydrogen peroxide, (C) acetic acid, and (E) a chelating agent. Add and dilute catalase enzyme and (F) water to contain (A) peracetic acid concentration of 100-10000 ppm, (D) catalase enzyme 0.01-250 ppm, (E) chelating agent 1-1000 ppm, and The mass ratio (A) / (B) of (A) peracetic acid and (B) hydrogen peroxide is 1 or more, and the mass ratio (D) / (E) of (D) catalase enzyme and (E) chelating agent is 0. It may be adjusted to be 0.002-10.

  As the catalase enzyme used in the present invention, those originating from microorganisms can be used, preferably catalase originating from the genus Aspergillus or Micrococcus, more preferably a catalase originating from Aspergillus niger Is mentioned. Catalase enzyme may be in the form of an aqueous solution or powder, and the stabilizing components used for the purpose of improving the stability in aqueous solution or when powdered include methanol, ethanol, glycerin, sorbitol, maltitol, mannitol, cyclodextrin, Examples include dextrin, cysteine, acetylcysteine, glutathione, tocopherol, ascorbic acid, thioglycolic acid or salts thereof, and sodium citrate. Preferable stabilizing components include ethanol and dextrin, and more preferable stabilizing components include dextrin. Salts derived from strong acids such as hydrochloric acid, nitric acid, sulfuric acid, sulfonic acids and the like preferably have less salts derived from strong acids in order to affect the corrosion of the equipment in the environment of use.

  In the disinfectant composition of the present invention, the concentration of peracetic acid in the component (A) is 100 to 10,000 ppm, preferably 500 to 5000 ppm, and more preferably 1000 to 3000 ppm. If the peracetic acid concentration of component (A) is less than 100 ppm, sterilization against peracetic acid-resistant bacteria may be reduced, and if it exceeds 10000 ppm, it may affect the corrosion of the equipment and is costly. It is not preferable.

  The hydrogen peroxide concentration of the component (B) is preferably less than 2000 ppm, more preferably less than 200 ppm, and even more preferably less than 20 ppm. The lower limit of hydrogen peroxide is preferably 0.1 ppm, and the hydrogen peroxide concentration in the disinfectant composition of the present invention is most preferably 0.1 to 10 ppm. If it exceeds 2000 ppm, sterilization against peracetic acid-resistant bacteria may be reduced.

  The acetic acid concentration of the component (C) is preferably 100 to 20000 ppm, more preferably 500 to 10000 ppm, and further preferably 1000 to 6000 ppm. When the concentration of acetic acid in component (C) is less than 100 ppm, the effect of removing inorganic stains may be reduced, and if it exceeds 20000 ppm, the odor of acetic acid becomes intense and handling becomes difficult and packing of equipment This is not preferable because it may cause swelling and deterioration of the material.

  The component (D), catalase, is blended in an amount of 0.01 to 250 ppm, preferably 0.05 to 20 ppm, more preferably 0.1 to 10 ppm, and more preferably 0.5 to 5 ppm. Further preferred. When the blending amount of the component (D) is less than 0.01 ppm, the decomposition speed of hydrogen peroxide decreases, so that the disinfection to Paenibacillus genus bacteria may decrease, and if it exceeds 250 ppm, It is not preferable because the degradation product of catalase affects the stability of peracetic acid and aggregates resulting from the catalase enzyme are generated.

  Examples of the chelating agent for component (E) include polyphosphoric acid, organic phosphonic acid, phytic acid, ethylenediaminetetraacetic acid, diethylenetriaminepentaacetic acid, rodankari, polyaminocarboxylic acid, and salts thereof. Examples of polyphosphoric acid include picolinic acid, dipicolinic acid, pyrophosphoric acid, tripolyphosphoric acid, tetrapolyphosphoric acid, and polyphosphoric acid such as hexapolyphosphoric acid. Examples of organic phosphonic acid include 1-hydroxyethylidene-1, Examples include 1-diphosphonic acid, 2-phosphonobutane-1,2,4-tricarboxylic acid, aminotrimethylenephosphonic acid, and polyethylene polyamine-based phosphonic acids such as ethylenediaminetetra (methylenephosphonic acid) and diethylenetriaminepenta (methylenephosphonic acid). It is done. As a chelating agent, organic phosphonic acid and its salt are preferable, and 1-hydroxyethylidene-1,1-diphosphonic acid and 2-phosphonobutane-1,2,4-tricarboxylic acid are particularly preferable. (E) Although the chelating agent of a component is mix | blended 1-1000 ppm, it is preferable to mix | blend 5-500 ppm, It is more preferable to mix | blend 10-100 ppm, It is still more preferable to mix | blend 15-50 ppm. (E) When the compounding amount of the chelating agent is less than 1 ppm, the stability of peracetic acid may be lowered, and when it exceeds 1000 ppm, the catalase enzyme is deactivated, which is not preferable.

  Further, the water of component (F) is not particularly limited, and examples thereof include ion exchange water, soft water, pure water, tap water, and the like. From the viewpoint of the stability of the disinfectant composition, ion exchange water or pure water. Is preferred.

  The concentration ratio of the component (A) to the component (B) in the disinfectant composition of the present invention is (A) / (B) from the viewpoint of disinfecting to peracetic acid-resistant bacteria and stability of the disinfectant composition. ) Is 1 or more, preferably (A) / (B) is 2 or more, more preferably (A) / (B) = 26 or more, and most preferably (A) / (B) =. 100 or more. If (A) / (B) is less than 1, disinfection to peracetic acid-resistant bacteria may be reduced. The concentration ratio of the component (D) and the component (E) is (D) / (E) = 0.002 to 10 (mass ratio) from the viewpoint of disinfecting peracetic acid-resistant bacteria and suppressing the aggregates. ), Preferably (D) / (E) = 0.005-5, and more preferably (D) / (E) = 0.3-1. When (D) / (E) is less than 0.002, sterilization to peracetic acid-resistant bacteria may be reduced. When (D) / (E) exceeds 10, suppression of aggregates may occur. May decrease.

  Furthermore, in the disinfectant composition of the present invention, in addition to the component (A), component (B), component (C), component (D), component (E), component (F), (G) A nonionic surfactant may be blended as a component. As the nonionic surfactant of component (G), polyoxyalkylene alkyl ether, polyoxyalkylene dialkyl ether, polyoxyalkylene alkyl ester, polyoxyalkylene alkyl diester, polyoxyalkylene alkyl aryl ether, glycerin fatty acid ester or ethylene oxide thereof Additives, polyglycerin fatty acid ester, sorbitan fatty acid ester, polyoxyethylene sorbitan fatty acid ester, polyoxyethylene sorbit fatty acid ester, polyoxyethylene glycerin fatty acid ester, polyoxyethylene castor oil, polyoxyethylene hydrogenated castor oil, polyoxyethylene alkyl Amines, higher fatty acid alkanolamides, alkylglycosides, alkylamine oxides, N-octides Rupiroridon, and the like. Among these, polyoxyethylene alkyl ethers, polyoxyethylene alkenyl ethers, polyoxyethylene polyoxypropylene alkyl ethers, and polyalkylene oxide adducts such as random or block adducts of ethylene oxide and propylene oxide are preferred from the viewpoint of less foaming. A polyoxyalkylene alkyl ether type nonionic surfactant represented by the following general formula (I) or a polyoxyalkylene alkenyl ether type nonionic surfactant is more preferable.

(Chemical formula 1)
R- (OA) n-OH (I)
(In the formula, R represents an aliphatic hydrocarbon group having 8 to 18 carbon atoms, n represents a number of 3 to 50, and A represents an alkylene group of 2 to 4 carbon atoms.)

  Furthermore, an anionic surfactant may be blended as the component (H). As the anionic surfactant of component (H), higher alcohol sulfate ester salt, sulfurized olefin salt, higher alkyl sulfonic acid or salt thereof, α-olefin sulfonic acid or salt thereof, sulfated fatty acid or salt thereof, sulfonated fatty acid or A salt thereof, an alkyl phosphate ester salt, an α-sulfo fatty acid methyl ester salt, a polyoxyalkylene alkyl ether sulfate ester salt, a polyoxyalkylene alkyl phenyl ether sulfate ester salt, a fatty acid alkanolamide or a sulfate ester salt of an alkylene oxide adduct thereof, Sulfosuccinic acid ester, alkylbenzene sulfonic acid or its salt, alkyl naphthalene sulfonic acid or its salt, alkyl benzimidazole sulfonic acid or its salt, N-acyl-N-methyl taurine or its salt, acyloxyethane Examples thereof include sulfonic acid or a salt thereof, alkoxyethanesulfonic acid or a salt thereof, N-acyl-N-carboxyethyl taurine or a salt thereof, and alkyl or alkenylaminocarboxymethyl sulfate or a salt thereof, and an alkyl ether carboxylate. .

  The disinfectant composition of the present invention can be used as it is or after diluting the concentrated composition of the disinfectant composition with water. When transporting, since the concentrated composition of the disinfectant composition can reduce the transportation cost, the concentration of the component (A) is preferably 1 to 15% by weight, more preferably 3 to 14% by weight during transportation. More preferred is ˜13% by weight. The concentration of the component (E) is preferably 0.01 to 1.5% by weight, more preferably 0.03 to 1.0% by weight, and still more preferably 0.05 to 0.8% by weight. In the present invention, the water used for the preparation and dilution of the disinfectant composition is not particularly limited. Ion exchange water (water from which ions have been removed by an ion exchange resin), soft water, pure water (RO water, that is, reverse osmosis membrane) Water, distilled water, i.e. water distilled with a still), tap water and the like. Pure water, particularly RO water is preferred from the viewpoint of sterilization.

  The disinfectant composition of the present invention is prepared by mixing (B) hydrogen peroxide, (C) acetic acid, (E) chelating agent, and (F) water and leaving it in an equilibrium state (A) peracetic acid. , (B) Hydrogen peroxide, (C) Acetic acid, (E) Chelating agent, (F) A mixed equilibrium solution containing water is obtained, and (G) component and (H) component are further blended as necessary. Can be prepared. The disinfectant composition of the present invention may be prepared in a state where all the components are mixed in advance, but it is preferable to prepare it immediately before use in order to obtain a high disinfecting effect. As a method of preparation at the time of use, (A) component, (B) component, (C) component, (E) chelating agent, (F) water that has reached an equilibrium state, and predetermined amounts of other components, if necessary, are individually added. Can be prepared by dissolving in water, but from the viewpoint of simplicity, component (A), component (B) and component (C), component (E), component (F) and other components as desired It is preferable to use for the sterilization operation by mixing the first agent and the second agent immediately before use.

  Since the disinfectant composition of the present invention has a strong disinfecting property, it can be used in a wide range of fields in which contamination / contamination of fungi and microorganisms including Paenibacillus bacteria (food field, brewing field, medical field, agricultural field, etc.) ) Is useful for sterilization. For example, sterilization of containers and production lines in food processing factories such as dairy products, dairy products, beer, wine, sake, miso and soy sauce, disinfection and sterilization of medical equipment such as endoscopes, dialysis line cleaning, Control of pathogenic microorganisms in treated water of vegetables and fruits, control of microorganisms attached to seeds that cause plant disease or damage and seedling decay, reduction of pathogenic bacteria on the surface of poultry meat at poultry processing plants In particular, it can be suitably used for the sterilization of container packaging materials (PET bottles and caps) in the production of soft drinks containing PET bottles by the aseptic filling method, or the enhanced sterilization of the line.

  The temperature at the time of performing the sterilization treatment with the sterilizing agent composition of the present invention is 20 ° C. to 70 ° C., but more preferably 40 ° C. to 65 ° C. in order to improve the sterilization property, 50 ° C. More preferably, it is -59 degreeC. If the temperature of the disinfectant composition is less than 20 ° C, the disinfecting property against resistant bacteria such as Paenibacillus bacteria showing strong resistance to peracetic acid may deteriorate, and if it exceeds 70 ° C, Corrosiveness of stainless steel, which is a material of manufacturing equipment, and catalase enzyme may be deactivated.

  For the sterilization treatment, a method of spraying a sterilization solution onto the target object or immersing the target object in the sterilization solution can be adopted. It can be appropriately selected depending on the type and amount of dirt or inorganic dirt, the concentration of the sterilizing solution, and the like.

  Hereinafter, the present invention will be described in more detail with reference to examples.

Examples 1 to 69, Comparative Examples 1 to 6
Disinfectant compositions having the formulations shown in Tables 1 to 7 were prepared. The hydrogen peroxide concentration, peracetic acid concentration, and acetic acid concentration in each disinfectant composition were measured by the following methods. Moreover, about each disinfectant composition, the disinfection test and the corrosivity test were done. A result is combined with Tables 1-8 and shown.
For Examples 1-10, Examples 21-69, and Comparative Examples 5-6, (A) component, (B) component and (C) component, (E) component, (F) component and other components as desired. The first agent contained and the second agent containing the component (D) were mixed immediately before use and subjected to sterilization operation 5 minutes later. Examples 11 to 20 and Comparative Examples 1 to 4 Is used for the sterilization operation 60 minutes after mixing the first agent and the second agent immediately before use. Further, the disinfectant composition of Example 64 is (A) 10% peracetic acid, (B) 20% hydrogen peroxide, (C) 15% acetic acid, (E) 1-hydroxyethylidene-1, A concentrated composition containing 0.25% of 1-diphosphonic acid was diluted to 2% with pure water, and then (D) 10 ppm of catalase enzyme was added to the disinfectant composition, and after 5 minutes. The sterilization test was conducted at different test temperatures shown in Table 9. The results are shown in Table 9. In Tables 1 to 9, the blending ratio of each component is “ppm”. In addition, the values of (A) / (B) in Tables 1 to 9 are shown by rounding off one decimal place when it is not divisible.

(1) Hydrogen peroxide concentration measurement method

When measuring high-concentration (over 50.0 ppm) hydrogen peroxide In a 100 mL conical beaker, 1 mL of the prepared disinfectant composition (stock solution) was collected with a micropipette, and 10 mL of 10% sulfuric acid solution was added. Titrate with 0.1N potassium permanganate standard solution until it shows light pink color. Amount of potassium permanganate standard solution required for titration: A (mL), Factor of 0.1N potassium permanganate standard solution: F1 The hydrogen peroxide concentration was determined by the following formula (1).
(Equation 1)
Hydrogen peroxide concentration (w / v%) = A × 0.17 × F1 (1)

When measuring low concentration (0.1 to 50.0 ppm) hydrogen peroxide 100 mL of prepared disinfectant composition (stock solution) with a whole pipette in a 300 mL conical beaker and adding 10 mL of 10% sulfuric acid solution After that, the solution was titrated with a 0.05N potassium permanganate standard solution until a light pink color was obtained, and the amount of the potassium permanganate standard solution required for the titration: B (mL), the factor of the 0.05N potassium permanganate standard solution : From F2, the hydrogen peroxide concentration was determined by the following formula (2).
(Equation 2)
Hydrogen peroxide concentration (w / v%) = B × 0.01 × 0.085 × F2 (2)

(2) Peracetic acid concentration measurement method

When the composition contains high concentration (over 50.0 ppm) of hydrogen peroxide 10% iodine is added to the solution after titration of the hydrogen peroxide concentration based on the above method for measuring high concentration hydrogen peroxide. After adding 1 mL of potassium halide solution, titrate with 0.01N sodium thiosulfate standard solution until pale yellow, add 1 mL of 1% starch solution, titrate until dark blue color disappears, and 0.01N thiosulfate required for titration Sodium standard solution amount: C (mL) was determined. As a blank, 1 mL of purified water was collected and titrated in the same manner as described above to obtain a titration amount of 0.01 N sodium thiosulfate standard solution: C ₀ (mL). Standard amount of sodium thiosulfate required for sample titration: C (mL), Standard amount of sodium thiosulfate required for blank titration: C ₀ (mL), and factors of 0.01 N sodium thiosulfate standard solution: From F3, the peracetic acid concentration was determined by the following formula (3).
(Equation 3)
Peracetic acid concentration (w / v%) = (C−C ₀ ) × 0.038 × F3 (3)

When a low concentration (0.1 to 50.0 ppm) of hydrogen peroxide is contained in the composition, the solution after titrating the hydrogen peroxide concentration based on the method for measuring the low concentration hydrogen peroxide, After adding 1 mL of 10% potassium iodide solution, titrate with 1N sodium thiosulfate standard solution until pale yellow, add 1 mL of 1% starch solution, titrate until dark blue color disappears, 1N sodium thiosulfate required for titration Standard solution volume: D (mL) was determined. As a blank, 100 mL of purified water was collected and titrated in the same manner as described above to obtain a titration amount of 1N sodium thiosulfate standard solution: D ₀ (mL). Sodium thiosulfate standard solution volume required for sample titration: D (mL), sodium thiosulfate standard solution volume required for blank titration: D ₀ (mL), and factor of 1N sodium thiosulfate standard solution: F4 The peracetic acid concentration was determined by the following formula (4).
(Equation 4)
Peracetic acid concentration (w / v%) = (D−D ₀ ) × 0.01 × 3.8 × F4 (4)

(3) Acetic acid concentration measurement method Weighing the disinfectant composition: W (g) in a 200 mL conical beaker, adding 100 mL of pure water, and then using an automatic titrator (AT-610-PT, manufactured by Kyoto Electronics Co., Ltd.). Then, neutralization titration was performed with 0.5N sodium hydroxide standard solution. The acetic acid concentration was calculated from the required titration amount giving an inflection point (neutralization point) around pH 6.8 (t1). From the required amount of titration giving an inflection point around pH 6.8 (t1): T1 (mL), 0.5N sodium hydroxide standard solution factor: F5, and the mass of the disinfectant composition: W 5) Acetic acid concentration was determined by the equation.
(Equation 5)
Acetic acid concentration (w / v%) = 3 × T1 × F5 / W (5)

* 1: Bactericidal (spore bacteria, spore bacteria resistant to peracetic acid)
(A) Spore Bacteria for Test Bacillus subtilis (NBRC3134) was used as a spore bacterium.
As a spore bacteria resistant to peracetic acid, a strain obtained from Germspedia of Analyze J. Net, a comprehensive service site for analysis and testing, Paenibacillus fabisporus (order number: E123), collected at a beverage factory, isolated and identified Paenibacillus chibensis, a Paenibacillus bacterium, was used.
(B) Preparation of spore suspension The test strain was smeared on an SCD agar medium (Nissui Chemicals), cultured at 37 ° C, and after culturing, the spore was confirmed to be sufficiently formed by microscopic observation. did. 10 mL of sterilized pure water was put on the plate medium, and the colonies were scraped to collect the suspension. Centrifugal washing was performed 3 times at 10,000 rpm at 4 ° C. for 15 minutes. After centrifugation, an appropriate amount of sterilized pure water was added, and the number of bacteria was adjusted to about 2.0 to 9.0 × 10 ⁸ CFU / ml. Was heated in a water bath at 80 ° C. for 15 minutes to obtain a spore suspension.
(C) Preparation of Sterilization Neutralization Solution 10 g of soybean lecithin, 30 g of Tween 80, 1 g of L-histidine, 20 g of sodium thiosulfate, and 1 L of distilled water were dissolved by heating and cooled with stirring. Thereafter, 9 mL each was dispensed into a test tube with a screw cap and subjected to high-pressure sterilization (121 ° C., 20 minutes) to obtain a sterilized neutralizing solution. (D) Bactericidal test 100 mL of the agent composition was added. The Erlenmeyer flask containing each test disinfectant composition was placed on a magnetic stirrer in a water bath set to a predetermined temperature, and the temperature of the drug was raised while rotating the stirring bar of the Erlenmeyer flask. After confirming that each test disinfectant composition in the Erlenmeyer flask reached 59 ° C., 1 mL of the test bacteria suspension was added. Every 1 minute (drug contact time), 1 mL was taken into a test tube containing a sterilized neutralizing solution and stirred well. The mixed solution was mixed and solidified on an SCD agar medium and cultured at 37 ° C. for 2 days. After culturing, from the time until colony formation is not observed on the plate, sterilization (spore fungus, spore fungus resistant to peracetic acid) is evaluated according to the following criteria, and evaluation of Δ, ○, ◎ The thing was determined to be practical.
Evaluation criteria A: No colony formation is observed within 2 minutes after contact.
○: More than 2 minutes after contact, colony formation is not observed within 5 minutes.
Δ: More than 5 minutes after contact, no colony formation within 8 minutes.
X: Even if it exceeds 8 minutes after a contact, formation of a colony is seen.

* 2: Stainless Corrosion Test 50ml of disinfectant composition is put into a 100ml plastic bottle with cap and SUS304 panel (length x width x thickness = 75mm x 25mm x 1mm) is disinfected to half the area. Put it in the object and cap it. After raising the temperature of the capped plastic bottle to 59 ° C., it is allowed to stand for 7 days. After 7 days, the panel was taken out, washed with running water, dried at 105 ° C. for 2 hours, the appearance after drying was compared with the state before being immersed in the disinfectant composition, the corrosivity was judged, and Δ, ○ Were evaluated as practical.
Evaluation criteria ○: No change from before immersion △: Almost unchanged from before immersion ×: Corroded

* 3: Rubber packing compatibility 50mL of disinfectant composition is put into a glass bottle with a 100mL cap, and EPDM rubber panel (manufactured by Irumagawa Rubber Co., Ltd .: vertical x horizontal x thickness = 50mm x 25mm x 2mm (volume 2500 cubic millimeters)) ) So that it is fully immersed, and cap it. After raising the temperature to 65 ° C., the same temperature was maintained, and the mixture was allowed to stand for 24 hours, and then the EPDM rubber panel was taken out and washed with running water. After drying at 105 ° C. for 3 hours, the volume was measured, and the expansion coefficient was determined from the following formula (4).
Expansion rate = (volume after immersion−volume before immersion) / volume before immersion (4)
From the expansion coefficient, rubber packing compatibility was evaluated according to the following criteria, and those evaluated as Δ, ○, and ◎ were determined to be practical.
Evaluation criteria:
A: The expansion coefficient is less than 0.03.
○: Expansion coefficient is 0.03 or more and less than 0.05.
(Triangle | delta): An expansion coefficient is 0.05 or more and less than 0.1.
X: Expansion coefficient is 0.1 or more.

Claims (7)

A disinfectant composition containing (A) peracetic acid, (B) hydrogen peroxide, (C) acetic acid, (D) catalase enzyme, (E) chelating agent, and (F) water,
(A) Peracetic acid concentration of 100 to 10000 ppm,
(D) 1-10 ppm of catalase enzyme,
(E) 1 to 1000 ppm of chelating agent,
And the mass ratio (A) / (B) of (A) peracetic acid and (B) hydrogen peroxide is 1 or more,
(D) The mass ratio (D) / (E) of catalase enzyme to (E) chelating agent is 0.005 to 10,
A disinfectant composition characterized by the above. (E) component of the chelating agent, disinfectant composition according to claim 1 characterized in that an organic phosphonic acid and / or organic phosphonates. The chelating agent as component (E) is a compound selected from 1-hydroxyethylidene-1,1-diphosphonic acid, 2-phosphonobutane-1,2,4-tricarboxylic acid or a salt thereof. Item 3. The disinfectant composition according to item 1 or 2. Further, (G) a nonionic surfactant and / or, (H) an anionic surfactant disinfectant composition according to any one of claims 1 to 3, characterized that you containing 10~50000ppm . The disinfectant composition according to any one of claims 1 to 4, sterilization methods using temperature is characterized in that the warming sterilization treatment 20 ° C. to 70 ° C.. A sterilization method using the sterilization composition according to any one of claims 1 to 3 ,
Immediately before use, a first agent containing (A) peracetic acid, (B) hydrogen peroxide, (C) acetic acid, (E) a chelating agent and (F) water, and (D) a second agent containing a component sterilization method characterized by mixing to prepare the disinfectant composition is subjected to a sterilization operation. (D) catalase enzyme and (F) water are added to a concentrated composition containing at least one of (A) peracetic acid, (B) hydrogen peroxide, (C) acetic acid, and (E) chelating agent. Dilute
(A) Peracetic acid concentration of 100 to 10000 ppm,
(D) 1 to 10 ppm of catalase enzyme,
(E) 1 to 1000 ppm of chelating agent,
Contains and
(A) Mass ratio (A) / (B) of peracetic acid and (B) hydrogen peroxide is 1 or more,
(D) A method for producing a disinfectant composition, wherein the mass ratio (D) / (E) of catalase enzyme and (E) chelating agent is 0.005 to 10.