RU2569761C2 - Disinfectant composition - Google Patents

Abstract

FIELD: chemistry.

SUBSTANCE: invention relates to disinfection and can be used to disinfect medical articles, buildings, patient care items, laboratory vessels in case of bacterial, viral and fungal infections in treatment facilities, at public catering enterprises and public facilities.

EFFECT: chlorine dioxide-based disinfectant composition has high bactericidal, virucidal, fungicidal and sporicidal activity, is indifferent to materials and equipment, is soluble in water, exhibits activity at minimal concentrations of the active ingredient.

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Description

The invention relates to the field of disinfection and can be used to disinfect medical devices, facilities, patient care items, laboratory glassware for infections of bacterial, viral and fungal etiology in medical facilities, catering establishments, municipal facilities.

The group of traditional medical disinfectants includes aldehyde and oxygen-containing agents, chloroactive compounds, agents based on quaternary ammonium salts.

Known means for washing hemodialysis equipment and dialyzers. The disinfectant contains citric acid, peracetic acid and water [1].

For the disinfection of sanitary equipment, patient care items, medical devices, an agent is used, including alkyl dimethylbenzylammonium chloride, glyoxal, glutaraldehyde, lower alcohol, hydrophilic nonionic surfactant, trilon B, hydrophobic nonionic surfactant, tributyl or tricresyl phosphate [2 and ].

Known disinfectant composition [3], including hydrogen peroxide and an activator, which is used as one or more carboxylic acids and / or their derivatives, additionally contains a stabilizer, which is used as one or more oxygen-containing phosphorus compounds.

A disinfectant composition including hydrogen peroxide, an activator, which is used salicylic acid, a stabilizer, which is used sodium pyrophosphate and water, characterized in that the composition further comprises a corrosion inhibitor, which is used as an alkali metal silicate [4].

Disadvantages of traditional disinfectants:

- Aldehydes (formaldehyde, succinic aldehyde, glutaraldehyde) have a pronounced antimicrobial effect in combination with toxicity and a strong irritating effect, but due to their high activity these compounds are still relevant in medical practice.

- Oxygen-containing substances (hydrogen peroxide) are powerful oxidizing agents that dissolve biological substrates and have a destructive effect on most pathogenic organisms, but they are irritating and cause corrosion of metals.

- Ethyl and isopropyl alcohols of 60-90% concentration successfully fight against vegetative forms of bacteria, fungi, viruses, however, they are not able to effectively remove organic impurities, they do not clean the surface of processed products.

- Along with traditional means, surfactants from the surfactant series have recently been used: cationic, ampholytic, nonionic, anionic. This group also includes disinfectants based on quaternary ammonium compounds. The pluses of these substances include good cleaning properties, the absence of corrosive effects and unpleasant odors. However, due to the limited spectrum of antibacterial activity, they are mainly used as components of composite solutions.

A separate group of disinfectants should include chlorine dioxide-based products. Chlorine dioxide disinfectants belong to the group of oxidizing agents that do not emit active chlorine. It should be noted that the oxidizing potential of chlorine dioxide is higher than that of chlorine; therefore, when using ClO ₂ , a lower concentration of the working solution is sufficient. At the same time, chlorine dioxide is less corrosive and does not emit free chlorine into the atmosphere. The method of generating chlorine dioxide is based on the reaction between a solution of sodium chlorite and an acid solution. A chlorine dioxide based disinfectant is prepared by mixing two solutions - a base and an activator.

Closest to the technical nature of the proposed disinfectant composition is a disinfectant based on chlorine dioxide "Tristel Fusion" [5]. An aqueous solution of sodium chlorite is used as an activator, and a mixture of citric, boric and sorbic acids is used as a base. The disadvantage of this method is the introduction of expensive anti-corrosion additives and thickeners in the formulation, which significantly increases the cost of production.

The objective of the present invention is the creation of a disinfectant composition based on chlorine dioxide with the following characteristics: high bactericidal, virucidal, fungicidal and sporicidal activity, indifferent to materials and equipment, soluble in water, is active at minimal concentrations of the active principle, exposure is the shortest, has a low exposure cost.

The technical result is achieved in that the liquid disinfecting composition consists of an activator (an aqueous solution of sodium chlorite), a base (an aqueous solution of glyceric acid), a thickener (glycerin) and a corrosion inhibitor (phosphoric acid and benzotriazole). The proposed disinfectant composition is obtained by mixing 50 ml of the base and 50 ml of activator and subsequent dissolution in 500-5000 ml of distilled water. The working solution of chlorine dioxide is ready for use immediately after receipt, is used once and is not subject to storage.

The results obtained indicate a high disinfecting effectiveness of the agent for various test objects contaminated with gram-positive and gram-negative bacteria, fungi and viruses in concentrations of chlorine dioxide of 100-1120 ppm with an exposure of 5 minutes, regardless of the type of microorganism and object of disinfection.

The execution of the method

For testing, a number of working disinfecting solutions were prepared with different concentrations of the test composition by dissolving it in water. The surface of the test objects was treated with working disinfectant solutions with various concentrations of the composition in the working solution.

Preparation of working solution

A working solution of the product is prepared in a container of any material by mixing with distilled water. A solution of activator and base is poured into the container. The resulting solution was stirred for 60 seconds, which was then poured into 500-5000 ml of distilled water. The working solution of chlorine dioxide is ready for use immediately after receipt. The solution is used once and is not subject to storage.

Determination of the mass fraction of chlorine dioxide in the working solution

100 ml of 10% sulfuric acid are added to a 250 ml volumetric flask. Add 10 ml of a 10% aqueous potassium iodide solution to the flask with sulfuric acid and mix. To the resulting mixture add 20 ml of the prepared working solution of chlorine dioxide - the solution should turn brown. A 0.1 N sodium thiosulfate solution is added to the burette. Titrate the resulting solution with sodium thiosulfate to the point of discoloration.

Mass fraction of chlorine dioxide (X) is calculated by the formula:

where V ₁ - the volume of the sodium thiosulfate solution, which went to the titration, ml;

1.35 - conversion factor;

V ₂ - the volume of the test product, ml

The result is calculated in mg of chlorine dioxide per liter, which is equivalent to 1 ppm.

Example 1

Base - volume 50 ml

Mass fraction of sodium chlorite - 2.1%

Activator - volume 50 ml

Mass fraction of glyceric acid - 5%

Mass fraction of phosphoric acid - 1.7%

Mass fraction of glycerin - 0.8%

Mass fraction of benzotriazole - 0.16%

To prepare the working solution, the mixture of the base and activator is poured into 5 l of distilled water. The concentration of chlorine dioxide in the working solution is 100-120 ppm.

A study of disinfecting properties was carried out in accordance with the methods described in the collection [6].

The following strains were used as test microorganisms: Escherichia coli 1257, Staphylococcusaureus 906, Pseudomonasaeruginosa 15442, Candida albicans 15, Trichophytongypseum, Mycobacterium B5, hepatitis B virus, type 1 poliovirus, Sabin strain, vaccine.

As test objects contaminated with test microorganisms, various types of surfaces, medical devices, patient care items, utensils, including laboratory ones, were used.

Initial dissemination of test objects: Escherichia coli - (1.6 ± 0.1) × 105 KOE / cm ² , Staphylococcusaureus - (1.5 ± 0.2) × 105 KOE / cm ² , Pseudomonasaeruginosa - (1.6 ± 0.1) × 105 KOE / cm ² , Candida albicans - (1.6 ± 0.1) × 105 KOE / cm ² .

The criterion for the effectiveness of disinfection for bacterial and fungal infections: surfaces - at least 99.99%, other test objects - at least 100%, with viral infection - at least 100%.

Processing method: rubbing, irrigation, immersion in a working solution of the product or soaking.

Tests for the corrosive effects of the compounds on medical devices made of metal consisted in the fact that in the working solutions of the agent were placed samples of medical products from metals and kept in it for a continuously specified time at a given temperature. Then, visually, as well as by the change in mass (corrosion loss) of the samples, the corrosion effect was determined.

Test results: high disinfecting effectiveness of the specified composition for various test objects contaminated with gram-positive and gram-negative bacteria, fungi and viruses at a concentration of chlorine dioxide of 100-120 ppm at an exposure of 5 minutes, regardless of the type of microorganism and object of disinfection.

The working solution of this composition does not cause corrosion of medical devices made of metal.

Example 2

Base - volume 50 ml

Mass fraction of sodium chlorite - 2.1

Activator - volume 50 ml

Mass fraction of glyceric acid - 5%

Mass fraction of phosphoric acid - 1.7%

Mass fraction of glycerin - 0.8%

Mass fraction of benzotriazole - 0.16%

To prepare the working solution, the mixture of the base and activator is poured into 2.5 liters of distilled water. The concentration of chlorine dioxide in the working solution is 220-240 ppm.

The test results of the disinfectant effectiveness of the agent at a concentration of chlorine dioxide of 220-240 ppm are similar to example 1.

The working solution of this composition does not cause corrosion of medical devices made of metal.

Example 3

Base - volume 50 ml

Mass fraction of sodium chlorite - 2.1%

Activator - volume 50 ml

Mass fraction of glyceric acid - 5%

Mass fraction of phosphoric acid - 1.7%

Mass fraction of glycerin - 0.8%

Mass fraction of benzotriazole - 0.16%

To prepare the working solution, the mixture of the base and activator is poured into 1 liter of distilled water. The concentration of chlorine dioxide in the working solution is 580-600 ppm.

The test results of the disinfectant effectiveness of the agent at a concentration of chlorine dioxide of 580-600 ppm are similar to example 1.

The working solution of this composition does not cause corrosion of medical devices made of metal.

Example 4

Base - volume 50 ml

Mass fraction of sodium chlorite - 2.1%

Activator - volume 50 ml

Mass fraction of glyceric acid - 5%

Mass fraction of phosphoric acid - 1.7%

Mass fraction of glycerin - 0.8%

Mass fraction of benzotriazole - 0.16%

To prepare the working solution, the mixture of the base and activator is poured into 750 ml of distilled water. The concentration of chlorine dioxide in the working solution is 780-800 ppm.

The test results of the disinfectant effectiveness of the agent at a concentration of chlorine dioxide of 780-800 ppm are similar to example 1.

The working solution of the specified composition does not cause corrosion of medical devices made of metal.

Example 5

Base - volume 50 ml

Mass fraction of sodium chlorite - 2.1%

Activator - volume 50 ml

Mass fraction of glyceric acid - 5%

Mass fraction of phosphoric acid - 1.7%

Mass fraction of glycerin - 0.8%

Mass fraction of benzotriazole - 0.16%

To prepare the working solution, the mixture of the base and activator is poured into 500 ml of distilled water. The concentration of chlorine dioxide in the working solution is 1100-1120 ppm.

The test results of the disinfectant effectiveness of the agent at a concentration of chlorine dioxide of 1100-1120 ppm are similar to example 1.

The working solution of the specified composition does not cause corrosion of medical devices made of metal.

Results:

1. The effectiveness of disinfecting with solutions of the compositions of the funds (examples 1-5) of various test objects:

- in case of bacterial (except tuberculosis) infections - 99.99% -100% when the concentration of chlorine dioxide in the working solution is 100-1120 ppm and the disinfection time is 5 minutes;

- with tuberculosis - 99.99% -100% when the concentration of chlorine dioxide in the working solution is 100-1120 ppm and the disinfection time is 5 minutes;

- for candidiasis - 99.99% -100% when the concentration of chlorine dioxide in the working solution is 100-1120 ppm and the disinfection time is 5 minutes;

- for viral infections - 99.99% -100% when the concentration of chlorine dioxide in the working solution is 100-1120 ppm and the disinfection time is 5 minutes.

2. Corrosive effect on medical devices made of metal

The test on the indicator "Corrosion on medical devices made of metal" showed that the working solutions of the compositions of examples 1-5 do not cause corrosion.

Literature:

1. RF patent 2286776. Disinfectant detergent for medical devices / Smirnov A.V., Laseba V.A., Sapozhnikov DB 11/10/2006.

2. RF patent 2272652. Disinfectant and sterilizing agent / FSUE SSC Niopik. 03/27/2006.

3. RF patent 2210387. Disinfectant composition / Parfenova T. A., Kartavchenko A. V., Osmolovsky A. 08/20/2003.

4. RF patent 2371917. Disinfectant composition / Borodyansky LI, Smorugova O.D. 11/10/2009.

5. Patent US20100314267. Decontamination system / Tristel PLC. 12/10/2010.

6. Guide P. 4.2.2643-10 “Methods of laboratory research and testing of disinfectants to assess their effectiveness and safety”, Moscow. 01/06/2011.

Class of compounds: using chemicals, the chemical composition of materials used in disinfection, sterilization or deodorization containing halogen.

Claims (1)

A liquid disinfectant composition, characterized in that it is obtained by mixing 50 ml of an aqueous solution of the activator and 50 ml of an aqueous solution of the base, and then dissolving the aqueous solution of the activator and the base in 500-5000 ml of distilled water,
while aqueous solutions of the activator and the base contain ingredients in the following ratios, mass. %:
activator:
sodium chlorite - 2.10
water - the rest;
base:
glyceric acid - 5.00
phosphoric acid - 1.70
glycerin - 0.80
benzotriazole - 0.16.