KR101732257B1

KR101732257B1 - Eco-friendly oxygen radical composition for sterilization, disinfection and deodorization comprising extracts of thymus vulgaris l.

Info

Publication number: KR101732257B1
Application number: KR1020150088149A
Authority: KR
Inventors: 정성필
Original assignee: 정성필
Priority date: 2015-06-22
Filing date: 2015-06-22
Publication date: 2017-05-04
Also published as: KR20160150332A

Abstract

The present invention relates to a composition for sterilizing, disinfecting and reducing odor of a medical device, food cooking apparatus, human body and the like in which harmful microorganisms are present, and contains a peroxide and a thymus vulgaris extract. The composition for sterilization and odor reduction according to the present invention is harmless to the human body, has high odor reducing power and sterilizing power, and has a long useful life.

Description

FIELD OF THE INVENTION [0001] The present invention relates to an oxygen-free sterilizing / disinfecting and odor-reducing oxygen radical composition,

The present invention relates to a sterilizing / disinfecting composition which can be used for removing harmful bacteria existing in the human body, agricultural products, foods, cooking utensils, medical instruments, and air conditioner filters.

Peroxide refers to an oxide containing a -2-valent O ₂ group, and is used as a main component of a sterilizing, disinfecting and cleaning composition. Peroxyacetic acid, which is one of the peroxides, is expressed as CH ₃ COOOH, has high reactivity and is known to have the property of killing various kinds of bacteria with a few drops of oxygen radicals have. The hydrocarbon end of peroxyacetic acid easily penetrates into microbial cells such as bacteria and decomposes SS and SH bonds inside and outside of microorganism to induce rapid and effective antimicrobial activity. When acetic acid peroxide is brought into contact with an organic substance, oxygen radicals at the atomic level are generated as shown in Reaction Scheme 1 below, and the generated oxygen radicals oxidize the organic substances to be contacted, thereby exerting a sterilizing effect.

[Reaction Scheme 1]

_{CH 3 COOOH + H 2 O ↔} CH 3 COOH + · O 2

Therefore, peroxyacetic acid is widely used as a cleaning agent for medical devices to remove pathogenic microorganisms, and is used in various fields such as air conditioner filters, cooking device cleaners, and hand cleaners.

Peroxyacetic acid can be prepared by continuously feeding hydrogen peroxide and acetic acid in a water-soluble reaction medium containing hydrogen peroxide, acetic acid and an acid catalyst, and continuously distilling the distillate with concentrated peroxyacid from the reaction medium. Also, Korean Patent Registration No. 10-0597092 discloses a method for obtaining peroxyacetic acid by reacting acetic acid with hydrogen peroxide as shown in Reaction Scheme 2 below.

[Reaction Scheme 2]

_{CH3COOH + H 2 O 2 ↔ CH} 3 COOOH + H 2 O

Conventionally, glutaraldehyde and chlorinated compounds were used as the main components of the sterilizing / disinfecting composition for medical devices, but there was a problem that corrosion of medical equipment, induction of allergy to human body, and residual harmful substances were left. Therefore, peroxoacetic acid which is harmless to the human body is currently in the spotlight. However, peroxyacetic acid has an unstable chemical structure and is easily decomposed into acetic acid, hydrogen peroxide (which is then decomposed into oxygen and water). Therefore, a sterilizing / disinfecting composition containing peroxyacetic acid as an active ingredient should be used only once after preparation, or within 1 to 3 days even if preserved after preparation. This is because the peroxide acetic acid contained in the sterilizing / disinfecting composition is decomposed and the initial concentration can not be maintained.

In order to prolong the useful life of such a product, Korean Patent Registration No. 10-0426937 discloses a method for producing a metal salt such as sodium orthophosphate, potassium orthophosphate, sodium pyrophosphate, potassium pyrophosphate, sodium polyphosphate, The contents of adding phosphate and nonionic surfactant to peroxyacetic acid are disclosed. Korean Patent Publication No. 10-0426937 claims that the disinfecting / disinfecting composition containing peroxyacetic acid is extended for 7 days, but the remaining percentage of peroxyacetic acid after 5 days is less than 49%, which is problematic in the period of use. Generally, peroxyacetic acid is contained in an extremely small amount of about 0.2 to 0.35 g per 100 ml of the sterilizing / disinfecting composition. This is because if the peroxyacetic acid is contained in a large amount in the sterilizing / disinfecting composition, the inherent odor of the peroxide acetic acid is severe.

Korean Patent Registration No. 10-0426937 (Acetic acid composition for sterilization / disinfection)

Disclosed is an oxygen radical composition for sterilization, disinfection, and odor reduction. It is a harmful substance to a human body, but contains a large amount of peroxyacetic acid, thereby increasing sterilization / disinfection effect. And an oxygen radical composition for odor reduction.

An oxygen radical composition for sterilization, disinfection and odor reduction according to an embodiment of the present invention includes peroxide and time herb ( Thymus vulgaris L. ) extract. The peroxide includes peroxyacetic acid, hydrogen peroxide and peroxyoctanoic acid. Preferably, the peroxide comprises 15 to 30% by weight of hydrogen peroxide (H ₂ O ₂ ) and acetic acid (CH ₃ COOH), and the time-hub extract may contain 1 to 15% by weight based on the total weight% of the composition. The sterilizing / disinfecting composition may further include selenium (Se), and the selenium may contain 0.01 to 3% by weight based on the total weight% of the composition.

Disclosure of Invention The present invention relates to an oxygenetracetic composition for sterilizing, disinfecting, and reducing odor, which increases the content of peroxides to increase the efficiency of sterilization and disinfection and removes odor, which can be generated as the content of peroxide increases, . In addition, the time-herbal extract has its own sterilization and disinfection effect, and it can improve the sterilization and disinfection effect in addition to the peroxide. The sterilizing / disinfecting composition according to the present invention can inhibit the generation of radical oxygen radicals of peroxides when selenium is further added, thereby extending the useful life of the sterilizing / disinfecting composition.

FIG. 1 is an image (left side) immediately after treating the composition according to an embodiment of the present invention with E. coli and an image after 30 minutes (right side).
FIG. 2 is an image (left) immediately after treatment of Staphylococcus aureus according to an embodiment of the present invention and an image after 30 minutes (right). FIG.

The following detailed description of the invention is set forth to be in a specific embodiment, in which the invention may be practiced, and will be described in sufficient detail to enable those skilled in the art to practice the invention. It should be understood that the various embodiments of the present invention are different, but need not be mutually exclusive. Therefore, the following detailed description is not to be taken in a limiting sense, and the scope of the present invention is not limited to the description in the claims and all the equivalents thereof

According to an embodiment of the present invention, an oxygen-sensitive sterilizing / disinfecting and odor-reducing oxygen radical composition includes peroxide and time-herb extract.

The peroxide (Peroxide) is decomposed as the oxide with an O ₂ -2 valent generates Oxygen radicals (O ₂ ·) of free radicals. For example, the peroxide may include, but is not limited to, compounds of the formulas I through V as shown in Table 1 below.

division The Formula I

(II)

(III)

Formula IV

Formula V

Wherein at least one of R, R 'in the formulas (I) to (V) is C ₁ to C ₆ alkyl, C ₁ to C ₆ cyclic alkyl, N, K, Ca, Na, Cl, S, C ₁ -C ₆ alkyl or C ₁ -C ₆ cyclic alkyl; N, K, Ca, Na, Cl, S, H, OH.

The peroxide may be selected from peroxyacetic acid, hydrogen peroxide (H ₂ O ₂ ), and peroxyoctanoic acid. The oxygen radical composition of the present invention may contain 30 to 60% by weight of peroxide to improve the sterilizing / disinfecting effect. Preferably, the peroxide may contain a total composition weight percent hydrogen peroxide, 15 to 30% by weight and acetic acid (CH ₃ COOH) 15 to 30% by weight. As hydrogen peroxide and acetic acid are mixed and reacted, peracetic acid and water can be formed (see Scheme 2 above).

The peroxoacetic acid composition of the present invention contains a large amount of peroxide, while conventionally conventionally contained peroxyacetic acid is contained in an extremely small amount of about 0.2 to 0.35 g per 100 ml of the whole commercialized composition. Due to its inherent nature, the presence of large quantities of peroxides results in the irritating odor of the perennial inherent smell of peroxides.

The oxirane radical composition of the present invention may further comprise a Thymus vulgaris extract in order to remove the odor generated as a result of containing a large amount of the peroxide and to lower the irritation. The time hub is also called time or dime (Thyme), also known as thyme. Time Hub is called thyme because its fragrance goes up to the white prairie. It is cultivated only in the Mediterranean Sea, and now it is cultivated in various regions all over the world such as Europe, West Asia and North Africa spreading its awareness about its flavor and aroma. have.

The time-herbal extracts contained in the oxirane radical composition of the present invention remove irritating odors generated due to a large amount of peroxides. Time-herbal extracts obtained by distilling time-hubs with water vapor have been reported to contain phenolic compounds called thymol and carvacrol, which are highly sterilizable [Journal of Food, Agriculture & Environment Vol.5 ): 92-94. 2007], which is reported to be effective for sterilization and disinfection with peroxide. The time hub has the characteristic that its unique fragrance and flavor does not change even if it is dried, and it can be used with the peroxide after purifying it as a liquid extract to minimize the inherent irritating odor of the peroxide.

The time-hub extract may be contained in an amount of 1 to 15% by weight based on the total weight% of the composition. The amount of the time herb extract may be varied to remove the odor according to the content of the peroxide. However, when the time herb extract is less than 1% by weight based on the total weight% of the composition, odor of peroxides is generated, The fragrance of time-herbal extract is too strong and can be a problem.

Specifically, when 15% by weight of hydrogen peroxide and 15% by weight of acetic acid are contained relative to the total weight% of the composition, the time herb extract must be contained in an amount of 1% by weight or more to remove the offensive smell. On the other hand, when 30% by weight of acetic acid is contained in 30% by weight of hydrogen peroxide with respect to the total weight% of the composition, 15% by weight of the time herb extract must be contained to remove the offensive smell.

The oxigen radical composition of the present invention may further comprise selenium. Selenium removes oxigen radicals with strong antioxidant power. Selenium can prolong the shelf life of the sterilizing / disinfecting composition by delaying the amount of the oxigen radical which is generated suddenly when the peroxide is in contact with the organic matter. Specifically, the time-herbal extract can be used to solve the inherent odor problem of peroxides, thereby making it possible to prepare an oxygen radical composition containing a large amount of peroxide. In the state where a large amount of peroxide is contained, peroxides come into contact with organic substances to release oxigen radicals Selenium inhibits and delays the action so that the shelf life can be prolonged.

Preferably, the selenium is present in an amount of 0.01 to 3 wt% . When the amount of selenium is less than 0.01% by weight based on the total weight% of the composition, it is not possible to control the release of oxigen radicals of peroxides contained in large amounts, and thus the useful life can not be extended. When the selenium content exceeds 3% by weight based on the total weight% of the composition, the release of oxigen radicals of the peroxide is markedly reduced, so that the sterilizing / disinfecting effect is hardly observed.

The sterilizing / disinfecting composition of the present invention may further comprise an organic acid. The organic acid can increase the sterilizing / disinfecting effect in addition to the peroxide and the time herb extract. For example, the organic acid may include at least one of citric acid, malic acid, succinic acid, tartaric acid, sorbic acid, benzoic acid, salicylic acid, dimethylbenzoic acid, ethylbenzoic acid, dimethylbenzoic acid and dihydroxybenzoic acid.

The sterilizing / disinfecting composition of the present invention may contain conventional preservatives, flavors, pigments, surfactants, diluents, alcohols, polyols, phosphate buffers, moisturizers, and purified water. In addition, the sterilizing / disinfecting composition of the present invention can be used for sterilizing / disinfecting Escherichia coli, Salmonella and Staphylococcus bacteria existing in the toilet in addition to the purpose of sterilizing / disinfecting pathogenic harmful microorganisms. Such as Shigella, Campylobacter, Yersinia enterocolitica, Listeria monocytogenes, Staphylococcus aureus, Clostridium, Celebrex, Can be used to disinfect / disinfect Bacillus cereus, Vibrio, Clostridium botulinum, bacillus, and Listeria monocytogenes. It can also be used to remove the heterocyclic amine, benzopyran, which remains in the cooking utensil.

Hereinafter, the sterilizing / disinfecting composition of the present invention will be described in detail with reference to examples of the present invention, but the present invention is not limited to these examples.

&Lt; Preparation of oxigen radical composition >

As shown in Table 2, the raw materials were prepared for each content.

division Raw material Content (relative to the total weight% of the composition) One Hydrogen peroxide 17 wt% 2 Acetic acid 30 wt% 3 Time herbal extract 4 wt% 4 Selenium 0.5 wt% 5 Citric acid 0.1 wt% 6 Malic acid 0.1 wt% 7 phosphate 0.05 wt% 8 Purified water Balance sum 100

1 and 2 were mixed, diluted to 8, and 4 to 7 were sequentially added and mixed to prepare an oxigen radical composition (hereinafter, referred to as an example composition).

<Experimental Example 1-Disinfection Test>

Example compositions sterilization efficiency of E. coli gram-negative bacteria (Escherichia coli, ATCC 25922), Gram-positive bacteria of Staphylococcus aureus (Staphylococcus aureus, ATCC 25922) to measure the progress of the sterilization experiment was included in this study.

After the test strains were cultured in the medium, the cultured test strains were diluted and adjusted to have an initial inoculum of 1 to 9 × 10 ⁵ CFU / ml and used for the sterilization experiment. Subsequently, the test strain was added to 20 ml of the composition of the example (0.2% v / v). When mixed and left at room temperature for 30 minutes, the number of bacteria was measured and the rate of sterilization reduction was confirmed based on the initial number of bacteria. The initial bacterial count was determined by comparing the sterilized physiological saline solution and the sterilization efficiency was compared by comparing it with the example composition.

The sterilization reduction rate (%) is derived by the following equation.

(%) = (A - B) / A X 100

[A: initial number of bacteria, B: number of bacteria after 30 minutes]

When the sterilization reduction rate is high, it means that the sterilizing / sterilizing composition is highly efficient in sterilizing and disinfecting the test strain.

The results of the sterilization test are shown in Table 3 below.

Test strain Number of initial bacteria (CFU / mL) Sterilization reduction rate (%) Escherichia coli
(Escherichia coli) 4.0 X 10 ⁵ 99.9% or more Staphylococcus aureus
(Staphylococcus aureus) 5.8 X 10 ⁵ 99.9% or more

Fig. 1 is an image (left side) immediately after treatment of the Example composition with E. coli (right side) and an image after 30 min (right side), Fig. 2 is an image (left side) immediately after treating the Example composition with Staphylococcus aureus (Right side).

As a result of an experiment on the bactericidal effect of the composition of Example of the present invention on Escherichia coli and Yellow Staphylococcus aureus, the reduction rate of sterilization was 99.9%. It was confirmed that the composition of the Example can remove almost all of E. coli and Staphylococcus aureus.

<Experimental Example 2 - Odor Removal Test>

0.2 ml of the Example composition was diluted with 19.8 ml of distilled water. This was repeated four times to prepare four example compositions.

Four compositions of the Examples were placed in four hermetically sealed reactors (capacity 5 L), 50 μmol / mol ammonia (NH ₄ ), 50 μmol / mol trimethylamine (CH ₃ ) ₃ N, 50 μmol hydrogen sulfide (H ₂ S) / mol, and methyl mercaptan (CH ₃ SH) 10 μmol / mol were injected into the reactor, respectively. After the sample gas was injected, the concentration of the sample gas after the elapse of the initial (0 minute), 30 minute, 60 minute, 90 minute and 120 minute was measured (referred to as sample concentration).

On the other hand, the experimental gas was put in the same manner as described above, and the experimental gas concentration was measured in the same manner over time (hereinafter referred to as the blank concentration).

The removal rate of the experimental gas for each time period was calculated by the following equation, and the measurement results were as shown in Table 4.

Gas removal rate (%) = {(Blank concentration - Sample concentration) / Blank concentration} x 100

time
lapse ammonia
(NH ₄₎ Trimethylamine
(CH _{3) 3} N Hydrogen sulfide
(H ₂ S) Methyl mercaptan
(CH ₃ SH) Blank concentration
(μmol / mol) Sample concentration
(μmol / mol) Deodorization rate
(%) Blank concentration
(μmol / mol) Sample concentration
(μmol / mol) Deodorization rate
(%) Blank concentration
(μmol / mol) Sample concentration
(μmol / mol) Deodorization rate
(%) Blank concentration
(μmol / mol) Sample concentration
(μmol / mol) Deodorization rate
(%) 0 minutes 50 50 0.0 50 50 0.0 50 50 0.0 10 10 0.0 30 minutes 49 Not detected 99.6 49 2 95.9 49 2 95.9 10 Not detected 99.0 60 minutes 49 Not detected 99.6 49 One 98.0 49 2 95.9 10 Not detected 99.0 90 minutes 49 Not detected 99.6 49 One 98.0 49 One 98.0 10 Not detected 99.0 120 minutes 49 Not detected 99.6 49 One 98.0 49 One 98.0 10 Not detected 99.0

* Detection limit of gas concentration 0.2 μmol / mol

In the case of ammonia and methyl mercaptan, ammonia and methyl mercaptan were completely removed after 30 minutes, and the deodorization rate was high. In addition, after 30 minutes, 95.9% of trimethylene amine was removed and 95.9% of hydrogen sulfide was removed.

From these results, it was confirmed that the time-hub extract added to the composition of the examples can deodorize the odorous gas.

While the present invention has been described in connection with what is presently considered to be practical exemplary embodiments, it is to be understood that the invention is not limited to the disclosed embodiments, but, on the contrary, It will be obvious to the person.

Claims

Disclosed is an oxygen radical composition for environmental sterilization, disinfection and odor reduction, comprising peroxide, Thymus vulgaris extract and selenium (Se).

The method according to claim 1,
Wherein the peroxide comprises at least one of peroxyacetic acid, hydrogen peroxide, and peroxyoctanoic acid. 2. The composition of claim 1, wherein the peroxide is at least one selected from the group consisting of peroxyacetic acid, hydrogen peroxide, and peroxyoctanoic acid.

The method according to claim 1,
Wherein the peroxide comprises 15-30 wt% of hydrogen peroxide (H ₂ O ₂ ) and 15-30 wt% of acetic acid (CH ₃ COOH) based on the total weight% of the composition. Composition.

The method according to claim 1,
Wherein the time-hub extract comprises 1 to 15% by weight, based on the total weight% of the composition, of an oxygene radical composition for environmental sterilization, disinfection and odor reduction.

delete

The method according to claim 1,
Wherein the selenium content is 0.01 to 3% by weight based on the total weight% of the composition.

The oxygen-scavenging, sterilizing and odor-reducing oxygen radical composition according to claim 1, which is characterized by sterilizing Escherichia coli and Staphylococcus aureus , is an oxygen-radical sterilizing / disinfecting and odor-reducing oxygen radical composition .

The environmental friendly sterilizing / disinfecting and odor-reducing oxygen radical composition according to claim ₁ reduces or eliminates ammonia (NH ₄ ), trimethylamine (CH ₃ ) ₃ N, hydrogen sulfide (H ₂ S) and methyl mercaptan (CH ₃ SH) Wherein said oxygen radical scavenging, disinfection and odor reduction are environmentally friendly.