JP2013210287A5 - - Google Patents
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- JP2013210287A5 JP2013210287A5 JP2012080755A JP2012080755A JP2013210287A5 JP 2013210287 A5 JP2013210287 A5 JP 2013210287A5 JP 2012080755 A JP2012080755 A JP 2012080755A JP 2012080755 A JP2012080755 A JP 2012080755A JP 2013210287 A5 JP2013210287 A5 JP 2013210287A5
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- particles
- calibration
- light generated
- detection
- intensity
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- 239000002245 particle Substances 0.000 claims description 52
- 238000001514 detection method Methods 0.000 claims description 31
- 239000010419 fine particle Substances 0.000 claims description 13
- 239000012530 fluid Substances 0.000 claims description 3
- 230000001678 irradiating Effects 0.000 claims description 2
- 238000003260 fluorescence intensity Methods 0.000 claims 9
- 238000000034 method Methods 0.000 claims 5
- 239000007788 liquid Substances 0.000 claims 3
- 239000011859 microparticle Substances 0.000 claims 1
Description
また、本技術に係る微小粒子分取装置は以下のような構成をとることもできる。
（１）サイズ及び蛍光強度が異なる２種以上のキャリブレーション粒子を含む流体が流路に通流されたマイクロチップ又はフローセルにレーザを照射して発生する光を検出する照射検出部と、該照射検出部に対する前記マイクロチップ又は前記フローセルの相対位置を変更する位置調整部と、全種の前記キャリブレーション粒子から発生する光の検出強度がより大きくなる位置への移動信号を前記位置調整部に出力する制御部と、を備える微小粒子分取装置。
（２）前記検出強度が、全種の前記キャリブレーション粒子から発生する光の検出強度の積算値のエリア平均値である上記（１）記載の微小粒子分取装置。
（３）前記制御部は、前記エリア平均値がより大きくなるエリア内において、前記キャリブレーション粒子のうちサイズが小さい種の粒子から発生する光の検出強度がより大きくなる第一の最適位置への移動信号を前記位置調整部に出力する上記（２）記載の微小粒子分取装置。
（４）前記制御部は、前記エリア平均値がより大きくなるエリア内において、前記キャリブレーション粒子のうちサイズが小さい種の粒子から発生する光の検出強度の積算値又は平均値の変動係数がより小さくなる第二の最適位置への移動信号を前記位置調整部に出力する上記（２）又は（３）記載の微小粒子分取装置。
（５）前記制御部は、前記第一の最適位置と前記第二の最適位置が異なる場合に、前記第二の最適位置への移動信号を前記位置調整部に出力する上記（４）記載の微小粒子分取装置。
（６）前記流路の一端に形成されたオリフィスから吐出される液滴に対して、前記照射検出部による前記キャリブレーション粒子から発生する光の検出時刻から所定時間経過後にレーザを照射する光源と、前記液滴から発生する光を検出する検出部と、を備え、前記制御部は、全種の前記キャリブレーション粒子から発生する光の検出強度の積算値、あるいは前記キャリブレーション粒子のうちサイズが大きい種の粒子から発生する光の検出強度の積算値、がより大きくなる経過時間をディレイタイムとして設定する上記（１）〜（５）のいずれかに記載の微小粒子分取装置。
In addition, the fine particle sorting apparatus according to the present technology may have the following configuration.
(1) An irradiation detection unit that detects light generated by irradiating a laser to a microchip or a flow cell in which fluids containing two or more kinds of calibration particles having different sizes and fluorescence intensities are passed through the flow path, and the irradiation A position adjustment unit that changes the relative position of the microchip or the flow cell with respect to the detection unit, and a movement signal to a position where the detection intensity of light generated from all types of calibration particles becomes larger is output to the position adjustment unit. A fine particle sorting device comprising a control unit.
(2) The fine particle sorting apparatus according to (1), wherein the detection intensity is an area average value of integrated values of detection intensity of light generated from all types of the calibration particles.
(3) In the area where the area average value is larger, the control unit is directed to the first optimum position where the detection intensity of light generated from the small-sized seed particle among the calibration particles is larger. The fine particle sorting apparatus according to (2), wherein a movement signal is output to the position adjustment unit.
(4) In the area where the area average value is larger, the control unit has an integrated value or a coefficient of variation of the average value of the detected intensity of light generated from a small-sized particle of the calibration particles. The fine particle sorting device according to (2) or (3) , wherein a movement signal to a second optimum position that decreases is output to the position adjustment unit.
(5) The control unit according to (4), wherein the control unit outputs a movement signal to the second optimum position to the position adjustment unit when the first optimum position and the second optimum position are different. Fine particle sorting device.
(6) a light source that irradiates a droplet discharged from an orifice formed at one end of the flow path with a laser after a predetermined time has elapsed from a detection time of light generated from the calibration particles by the irradiation detection unit; A detection unit that detects light generated from the droplets, and the control unit has an integrated value of detection intensities of light generated from all types of the calibration particles or a size of the calibration particles. The fine particle sorting device according to any one of (1) to (5), wherein an elapsed time when the integrated value of the detection intensity of light generated from a large type of particle becomes larger is set as a delay time.
Claims (20)
レーザ照射により前記マイクロチップ又は前記フローセルから発生する光を前記マイクロチップ又は前記フローセル上の複数の位置から検出する第一の信号取得手順と、
全種の前記キャリブレーション粒子から発生する光の検出強度がより大きくなる位置を特定する手順と、を含む微小粒子分取装置におけるキャリブレーション方法。 A liquid feeding procedure for flowing a fluid containing two or more kinds of calibration particles having different sizes and fluorescence intensities through a flow path formed in a microchip or a flow cell;
A first signal acquisition procedure for detecting light generated from the microchip or the flow cell by laser irradiation from a plurality of positions on the microchip or the flow cell;
And a procedure for specifying a position where the detection intensity of light generated from all kinds of the calibration particles becomes larger.
全種の前記キャリブレーション粒子から発生する光の検出強度の積算値、あるいは前記キャリブレーション粒子のうちサイズが大きい種の粒子から発生する光の検出強度の積算値、がより大きくなる経過時間を特定する手順と、を含む請求項１〜７のいずれか記載のキャリブレーション方法。 A liquid is emitted to a droplet discharged from an orifice formed at one end of the flow path after a predetermined time has elapsed from a detection time of light generated from the calibration particles flowing through the flow path, and the liquid A second signal acquisition procedure for detecting light generated from the drop;
Identifies the elapsed time when the integrated value of the detection intensity of light generated from all types of the calibration particles or the integrated value of the detection intensity of light generated from particles of a large size among the calibration particles becomes larger The calibration method according to any one of claims 1 to 7 , further comprising:
該照射検出部に対する前記マイクロチップ又は前記フローセルの相対位置を変更する位置調整部と、
全種の前記キャリブレーション粒子から発生する光の検出強度がより大きくなる位置への移動信号を前記位置調整部に出力する制御部と、を備える微小粒子分取装置。 An irradiation detection unit that detects light generated by irradiating a laser to a microchip or a flow cell in which fluids containing two or more kinds of calibration particles having different sizes and fluorescence intensities are passed through the flow path;
A position adjusting unit for changing the relative position of the microchip or the flow cell with respect to the irradiation detecting unit;
A fine particle sorting apparatus comprising: a control unit that outputs a movement signal to a position where detection intensity of light generated from all types of the calibration particles becomes larger to the position adjustment unit.
前記液滴から発生する光を検出する検出部と、を備え、
前記制御部は、全種の前記キャリブレーション粒子から発生する光の検出強度の積算値、あるいは前記キャリブレーション粒子のうちサイズが大きい種の粒子から発生する光の検出強度の積算値、がより大きくなる経過時間をディレイタイムとして設定する請求項１２〜１６のいずれか記載の微小粒子分取装置。 A light source that irradiates a laser after a predetermined time has elapsed from a detection time of light generated from the calibration particles by the irradiation detection unit with respect to a droplet discharged from an orifice formed at one end of the flow path,
A detection unit for detecting light generated from the droplets,
The control unit has a larger integrated value of detection intensity of light generated from all types of calibration particles, or an integrated value of detection intensity of light generated from particles of a large size among the calibration particles. The minute particle sorting device according to any one of claims 12 to 16, wherein the elapsed time is set as a delay time.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2012080755A JP5905317B2 (en) | 2012-03-30 | 2012-03-30 | Calibration method and apparatus for fine particle sorting apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2012080755A JP5905317B2 (en) | 2012-03-30 | 2012-03-30 | Calibration method and apparatus for fine particle sorting apparatus |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016052262A Division JP6237806B2 (en) | 2016-03-16 | 2016-03-16 | Fine particle fractionator |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2013210287A JP2013210287A (en) | 2013-10-10 |
| JP2013210287A5 true JP2013210287A5 (en) | 2015-02-26 |
| JP5905317B2 JP5905317B2 (en) | 2016-04-20 |
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| Application Number | Title | Priority Date | Filing Date |
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| JP2012080755A Active JP5905317B2 (en) | 2012-03-30 | 2012-03-30 | Calibration method and apparatus for fine particle sorting apparatus |
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| JP (1) | JP5905317B2 (en) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP6102783B2 (en) * | 2014-02-14 | 2017-03-29 | ソニー株式会社 | Particle sorting apparatus, particle sorting method and program |
| US20200032184A1 (en) | 2017-03-08 | 2020-01-30 | Sony Corporation | Cell sample liquid feeding bag, cell sample liquid feeding method, and cell sample liquid feeding device |
| WO2019207988A1 (en) | 2018-04-25 | 2019-10-31 | ソニー株式会社 | Microparticle fractionating device and microparticle fractionating method |
| JP6706011B2 (en) * | 2018-12-04 | 2020-06-03 | ソニー株式会社 | Particle sorting device, particle sorting method and program |
| JP6685057B1 (en) * | 2019-07-30 | 2020-04-22 | 株式会社Ｃｙｂｏ | Imaging flow cytometer, sorting method, and calibration method |
| EP4357754A2 (en) * | 2019-12-27 | 2024-04-24 | Thinkcyte, Inc. | Flow cytometer performance evaluation method and standard particle suspension |
| WO2023189819A1 (en) * | 2022-03-29 | 2023-10-05 | ソニーグループ株式会社 | Particle sorting system and particle sorting method |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH06288896A (en) * | 1993-03-31 | 1994-10-18 | Jasco Corp | Cell sorter |
| JP3875754B2 (en) * | 1995-11-17 | 2007-01-31 | シスメックス株式会社 | Standard solution for flow cytometer |
| JP3979304B2 (en) * | 2003-02-24 | 2007-09-19 | 日本光電工業株式会社 | Flow cell positioning method and flow cytometer with adjustable flow cell position |
| US7232687B2 (en) * | 2004-04-07 | 2007-06-19 | Beckman Coulter, Inc. | Multiple sorter monitor and control subsystem for flow cytometer |
| JP4488882B2 (en) * | 2004-12-14 | 2010-06-23 | 三井造船株式会社 | Flow cytometer and measurement method using flow cytometer |
| JP4509163B2 (en) * | 2007-10-26 | 2010-07-21 | ソニー株式会社 | Measuring method of fine particles |
| JP5487638B2 (en) * | 2009-02-17 | 2014-05-07 | ソニー株式会社 | Apparatus for microparticle sorting and microchip |
| JP5304456B2 (en) * | 2009-06-10 | 2013-10-02 | ソニー株式会社 | Fine particle measuring device |
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2012
- 2012-03-30 JP JP2012080755A patent/JP5905317B2/en active Active
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