WO2009108223A3 - Pathogen detection by simultaneous size/fluorescence measurement - Google Patents

Pathogen detection by simultaneous size/fluorescence measurement Download PDF

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Publication number
WO2009108223A3
WO2009108223A3 PCT/US2008/083052 US2008083052W WO2009108223A3 WO 2009108223 A3 WO2009108223 A3 WO 2009108223A3 US 2008083052 W US2008083052 W US 2008083052W WO 2009108223 A3 WO2009108223 A3 WO 2009108223A3
Authority
WO
WIPO (PCT)
Prior art keywords
fluorescence measurement
pathogen detection
simultaneous size
simultaneous
size
Prior art date
Application number
PCT/US2008/083052
Other languages
French (fr)
Other versions
WO2009108223A2 (en
Inventor
Michael Morrell
Gregory Scott Morris
Jien-Ping Jiang
Original Assignee
Biovigilant Systems, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biovigilant Systems, Inc. filed Critical Biovigilant Systems, Inc.
Publication of WO2009108223A2 publication Critical patent/WO2009108223A2/en
Publication of WO2009108223A3 publication Critical patent/WO2009108223A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Electro-optical investigation, e.g. flow cytometers
    • G01N15/1456—Electro-optical investigation, e.g. flow cytometers without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
    • G01N15/1459—Electro-optical investigation, e.g. flow cytometers without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals the analysis being performed on a sample stream
    • G01N2015/019—
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Electro-optical investigation, e.g. flow cytometers
    • G01N2015/1493—Particle size

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Dispersion Chemistry (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

A method and apparatus for detecting pathogens and particles in a fluid in which particle size and intrinsic fluorescence of a particle is determined. The classification of particles as biologic or inert particles using one or more thresholds is described.
PCT/US2008/083052 2007-11-09 2008-11-10 Pathogen detection by simultaneous size/fluorescence measurement WO2009108223A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US269207P 2007-11-09 2007-11-09
US61/002,692 2007-11-09

Publications (2)

Publication Number Publication Date
WO2009108223A2 WO2009108223A2 (en) 2009-09-03
WO2009108223A3 true WO2009108223A3 (en) 2010-01-21

Family

ID=41016636

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2008/083052 WO2009108223A2 (en) 2007-11-09 2008-11-10 Pathogen detection by simultaneous size/fluorescence measurement

Country Status (1)

Country Link
WO (1) WO2009108223A2 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9540604B2 (en) * 2011-07-29 2017-01-10 Honeywell Limited Apparatus and method for monitoring autotroph cultivation
KR101203955B1 (en) * 2011-12-27 2012-11-30 국방과학연구소 Method for dynamically raising alarm of biological particle monitor
JP6318026B2 (en) * 2014-06-26 2018-04-25 アズビル株式会社 Particle detection apparatus and particle detection method
JP6420976B2 (en) * 2014-06-26 2018-11-07 アズビル株式会社 Particle detection apparatus and particle detection method
JP2017067696A (en) * 2015-10-01 2017-04-06 シャープ株式会社 Fluorescence detection device
FI20165282A (en) * 2016-04-01 2017-10-02 Kemira Oyj A method and system for optimizing coagulation and / or flocculation in a water treatment process

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5290707A (en) * 1991-11-25 1994-03-01 The United States Of America As Represented By The Secretary Of The Army Method for detection of microorganisms
US5540494A (en) * 1994-06-03 1996-07-30 Purvis, Jr.; Norman B. Method and apparatus for determining absolute particle size, surface area and volume normalized fluorescence using forward angle light scatter intensity in flow cytometry
WO2005033283A2 (en) * 2003-09-30 2005-04-14 Singulex, Inc. Methods for enhancing the analysis of particle detection
US20050221497A1 (en) * 1992-02-24 2005-10-06 Carole Young Quality control method

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5290707A (en) * 1991-11-25 1994-03-01 The United States Of America As Represented By The Secretary Of The Army Method for detection of microorganisms
US20050221497A1 (en) * 1992-02-24 2005-10-06 Carole Young Quality control method
US5540494A (en) * 1994-06-03 1996-07-30 Purvis, Jr.; Norman B. Method and apparatus for determining absolute particle size, surface area and volume normalized fluorescence using forward angle light scatter intensity in flow cytometry
WO2005033283A2 (en) * 2003-09-30 2005-04-14 Singulex, Inc. Methods for enhancing the analysis of particle detection

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
"Searched for http://www.biovigilant.com/PDFs/Why Simultaneous Sizing and Fluorescence is Necessary for Microbial Detection 9-8-05-c.pdf.", WAYBACK MACHINE INTERNET ARCHIVE., 10 July 2009 (2009-07-10), Retrieved from the Internet <URL:http://web.archive.org/web/"/http://www.biovigilant.com/PDFs/Why%20Simultaneous%20Sizing%20and%20Fluorescence%20is%20Necessary%20for%20Microbial%20Detection%209-8-05-c.pdf> [retrieved on 20090710] *
"Why Simultaneous Sizing and Fluorescence is Necessary in Order to Do Microbial Detection.", BIOVIGILANT.COM BIOVIGILANT WHITE PAPER, 21 October 2006 (2006-10-21), Retrieved from the Internet <URL:http://www.biovigilant.com/PDFs/Why%20Simultaneous%20Sizing%20and%20Fluorescence%20is%20Necessary%20for%20Microbial%20Detection%209-8-05-c.pdf> [retrieved on 20090706] *
HAIRSTON ET AL.: "Design of an Instrument for Real-Time Detection of Bioaerosols Using Simultaneous Measurement of Particle Aerodynamic Size and Intrinsic Fluorescence.", JOURNAL OF AEROSOL SCIENCE, vol. 28, no. 3, 1997, pages 471 - 482 *
WEICHERT ET AL.: "Determination of Fluorescence Cross-Sections of Biological Aerosols.", PARTICLE & PARTICLE SYSTEMS CHARACTERIZATION, vol. 19, no. ISS.3, 1 July 2002 (2002-07-01), pages 216 - 222 *

Also Published As

Publication number Publication date
WO2009108223A2 (en) 2009-09-03

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