JP2013151448A - Cosmetic, elastase inhibitor, and antioxidant - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a cosmetic having an excellent elastase inhibition action and an antioxidant action and useful as an anti-aging cosmetic.SOLUTION: This cosmetic includes the extract of Fabales order, Leguminosae family, genus Havardia, Havardia Mexicana bark. The cosmetic is useful as an anti-aging cosmetic.

Description

The present invention relates to a cosmetic, an anti-aging cosmetic, an elastase inhibitor, and an antioxidant containing an extract of bark of the leguminous leguminous group, Habardia mexicana.

The skin is composed of epidermis, dermis, and subcutaneous tissue. Among them, the dermis is extremely important for maintaining the structure of the skin, and the skin is maintained by the dermal connective tissue formed from fibers such as elastin and collagen. It is believed that the loss of contraction force of this connective tissue impairs elasticity and causes wrinkles and skin talmi.

One of the causes of elastin degradation is considered to be degradation by elastase. By inhibiting the degradation, it is considered that skin tarmi, wrinkles, arteriosclerosis, etc. can be prevented or improved. In recent years, many skin external preparations and internal preparations that prevent or ameliorate this skin talmi, wrinkles, arteriosclerosis, etc. are known, and retinoic acid, α-hydroxy acid, retinol, etc. have been reported as active ingredients of skin external preparations. ing. However, some of these active ingredients have problems with skin irritation and stability, and others have insufficient effects (Patent Document 1).

In addition, as one of the causative substances in which wrinkles and skin talmi appear, so-called active oxygen is considered that oxygen in the atmosphere generates under the influence of ultraviolet rays or enzymes. Such active oxygen includes free radicals such as superoxide and hydroxy radical, and non-radicals such as singlet oxygen and hydrogen peroxide. These active oxygens oxidize fatty acids and produce peroxides. In addition, the peroxide and active oxygen produced have adverse effects such as cross-linking of collagen fibers, fragmentation of hyaluronic acid, partial cleavage of DNA helix, and tissue damage due to generation of chain radicals. As a result, it is said to promote aging of the living body such as skin wrinkles, loss of elasticity, and hair loss.

As countermeasures against these, there are many cosmetics that have an antioxidant effect such as ascorbic acid, tocophenol, and polyphenol, and that contain components that have an action of removing active oxygen (see, for example, Patent Documents 2 to 3). However, these raw materials are expensive and have problems such as poor stability.

Japanese Patent Laid-Open No. 2005-023021 International Publication No. 97/14705 pamphlet JP 2004-175856 A

The present invention relates to a cosmetic comprising an extract of the bark of the order of Fabales, Fabardae, Havardia, Mexicana, and extraction of the bark of the leguminous leguminous region, Habardia mexicana. An object of the present invention is to obtain an elastase inhibitor and an antioxidant containing a product extract as an active ingredient.

The present invention is a cosmetic comprising an extract of the bark of the leguminous leguminous Habardia genus Habardia mexicana.
The cosmetic is preferably an anti-aging cosmetic.
The present invention is also an elastase inhibitor containing as an active ingredient an extract of the leguminous leguminous Habardia genus Habardia mexicana bark.
This invention is also an antioxidant which uses the extract of the leguminous leguminous Habardia genus Habardia mexicana bark as an active ingredient.

According to the present invention, it is possible to provide a cosmetic that has excellent elastase inhibitory action and antioxidant action and is useful as an anti-aging cosmetic.

The cosmetic of the present invention contains an extract of the above-mentioned leguminous leguminous Habardia genus Habardia mexicana (hereinafter referred to as Habardia mexicana) bark. The above-mentioned Habardia Mexicana is a tree native to Mexico, and its bark is used in the present invention. The extract of Habardia mexicana bark can be extracted using an extraction solvent of water or a water-soluble solvent. Moreover, it can also hydrolyze after extracting using an extraction solvent. Moreover, after decomposing | disassembling using an enzyme, it can also extract using an extraction solvent.

Examples of the extraction solvent include water, physiological saline, alcohols (for example, lower alcohols such as methanol, absolute ethanol and ethanol, polyhydric alcohols such as propylene glycol and 1,3-butylene glycol), ketones such as acetone, and ethyl. Examples include ethers such as ether, dioxane, acetonitrile, and ethyl acetate, xylene, benzene, and chloroform. Among these, it is preferable to use water. These may be used alone or in combination of two or more.

After performing the above extraction, the target extract can be obtained by removing solids by filtration, centrifugation, decantation, or the like.

The extract of the barbaric mexicana bark does not lose its activity even if the pH is adjusted as necessary and the polymer is removed by gel filtration or ultrafiltration. In addition, the enzyme degradation product or a fractional component thereof may be concentrated by a method such as vacuum concentration, ultrafiltration or freeze concentration, or may be dry powdered by a method such as freeze drying, spray drying or flat plate drying. it can.

It has been clarified by the inventors that such an extract of Habardia mexicana bark has an action as an elastase inhibitor and an antioxidant. Since it has such an action as an elastase inhibitor and an antioxidant, it can improve wrinkles and sagging of skin by blending into cosmetics and is used as an active ingredient in anti-aging cosmetics can do. An elastase inhibitor and an antioxidant containing the above extract of the barbaric mexicana bark as an active ingredient are also one aspect of the present invention.

The cosmetic of the present invention preferably contains 0.01 to 10% by mass of the extract of the above-mentioned Habardia Mexicana bark. By setting it as the mixing | blending in the said range, the effect as an elastase inhibitor and an antioxidant can be obtained suitably. The blending amount is more preferably 0.1 to 2% by mass, and further preferably 0.5 to 1% by mass.

Examples of the cosmetic of the present invention include skin cosmetics, a skin patch sheet, and a cosmetic sheet. The cosmetic of the present invention includes quasi-drugs, designated quasi-drugs, external medicines, and the like, and the dosage form can be arbitrarily selected according to the purpose.

Specifically, skin lotions, emulsions, creams, ointments, gels, lotions, oils, packs, mists, skin cosmetics such as facial or body cosmetic sheets, foundations, lipsticks, blushers, eye shadows, eyeliners, mascaras And makeup compositions such as body soaps, hand soaps and soaps, and hair cosmetics such as shampoos, rinses, treatments and set agents.

In addition to the components described above, the cosmetic of the present invention can be appropriately selected from the following substances depending on the required efficacy and effects. For example, commonly used fats and oils, surfactants, moisturizers, whitening agents, pH adjusters, binders and thickeners, polyhydric alcohols, essential oils and perfumes, preservatives, antioxidants, ultraviolet rays Examples include various cosmetic ingredients such as absorbents, pigments, plant pulverized products and herbal medicines, inorganic salts and inorganic acids, cleaning agents, and emulsifiers.

Examples of the oils and fats are generally used in cosmetics, for example, soybean oil, almond oil, paraffin, cetanol, avocado oil, olive oil, jojoba oil, coconut oil, palm oil, nuka oil, egg yolk oil, castor oil, squalane. Vegetable oils such as lanolin, liquid paraffin, white petrolatum; animal fats such as beef tallow, pork tallow, horse tallow, turtle oil, mink oil, perserine oil, squalane; methylpolysiloxane, behenyl alcohol, glyceryl tricaprate, trioctanoic acid Examples thereof include synthetic fats and oils such as glyceryl, liquid paraffin, DHA, and EPA.

Examples of the surfactant include anionic surfactants such as sodium lauryl sulfate, triethanolamine lauryl sulfate, and diethanolamide laurate; cations such as stearyltrimethylammonium chloride, cetyltrimethylammonium chloride, and benzalkonium chloride. Non-ionic surfactants such as glyceryl monostearate, sorbitan monostearate, polyoxyethylene (20) sorbitan monostearate, polyoxyethylene hydrogenated castor oil, sucrose ester, fatty acid amide, polymer Surfactants, fluorosurfactants, silicone surfactants, polypeptide derivatives, natural surfactants and the like can be mentioned.

Examples of the humectant include synthetic humectants such as sodium pyrrolidone carboxylate and pantethein-S sulfonate; hyaluronic acid, elastin, keratin, dermatan sulfate, collagen, placental extract, royal jelly and hydrolysates thereof, and microbial fermentation broth Examples thereof include chitin, chitosan, pectin, glucosamine, N-acetylglucosamine and the like, natural humectants such as extracts derived from other plants and animals, vegetable oils and esterified products and derivatives thereof, and the like.

Examples of the whitening agent include ascorbic acid and salts and derivatives thereof, arbutin and derivatives thereof, placental extract, and extracts derived from other plants and animals.

Examples of the pH adjuster include organic acids such as citric acid, lactic acid, salicylic acid, tartaric acid, malic acid, benzoic acid, sodium citrate, fumaric acid, sodium monohydrogen phosphate, potassium dihydrogen phosphate, inorganic acids and the like. Examples include salts.

As the binder and thickener, generally used in cosmetics, for example, sodium carboxymethylcellulose, methylcellulose, casein, pectin, starch, sodium alginate, polyvinyl alcohol, polyvinylpyrrolidone, locust bean gum, agar, Examples thereof include carbopol, xanthan gum, bentonite and the like.

Examples of the polyhydric alcohols include glycerin, propylene glycol, sorbitol, polyethylene glycol, 1,3-butylene glycol, dipropylene glycol, polyoxyethylene glycerin (26E.O), and the like.

The essential oils and fragrances include lavender oil, jasmine oil, rose oil, lemon oil, orange oil, mint oil, thyme oil, ginger oil, fennel oil, cedar oil, hiba oil, raspberry oil, cypress oil, rose oil, Examples include natural and synthetic fragrances such as eucalyptus oil, camphor, peppermint oil, spearmint oil, geraniol, mandarin oil, spruce oil, citronellol, terpenes, coffee oil, tea oil, coconut oil, and raspberry ketone.

Examples of the preservative include paraoxybenzoic acid esters such as methyl paraben, ethyl paraben, propyl paraben, and butyl paraben, phenoxyethanol, ethanol, and dehydroacetic acid.

Examples of the antioxidant include EDTA4Na, EDTA2Na, butyloxytoluene, hydroxybutyltoluene, butylhydroxyanisole (BHA), dibutylhydroxytoluene (BHT), and derivatives thereof.

The ultraviolet absorber is not particularly limited as long as it has a property of selectively absorbing ultraviolet rays. For example, oxybenzone, oxybenzone sulfonic acid, tetrahydroxybenzophenone, dihydroxymethoxybenzophenone sodium sulfonate, dihydroxy Examples include methoxybenzophenone, dihydroxybenzophenone, sinoxate, methyl diisopropylcinnamate, octyl methoxycinnamate, glyceryl paraaminobenzoate, octyl paraaminobenzoate, and the like.

Examples of the pigment include bengara, yellow iron oxide, black iron oxide, titanium oxide, nylon powder, zinc oxide, sericite, mica, talc, carbon, medicinal charcoal and the like.

As the above plant pulverized products and herbal medicines, lemon peel, seaweed, cypress, hiba, fukkas, rice bran, shobu, ginger, licorice, chimpi, yuzu, touki, carrot, mint, keihi, ubai, mugwort, dokudami, Momonoha, Chamomile, Aloe, Jasmine, Rosehip, Lavender, Guava, Ougon, Cuco, Ganoderma, Elderberry, Ashitaba, Uguigi, Burdock, Black Sesame, Black Rice, Fujisan Ginseng, Ginseng, Taba Ginseng, Ginseng Grass, Gypsophila And the like, and the dried product thereof and the extract thereof.

Examples of the inorganic salts and inorganic acids include sodium chloride, sodium hydrogen carbonate, sodium carbonate, boric acid, borax, sodium sulfate, sodium sulfide, potassium sulfide, sodium nitrate, calcium nitrate, ammonium sulfate, sodium thiosulfate, calcium hydrogen phosphate , Potassium chloride, ammonium chloride, sodium phosphate, sodium hyposulfite, calcium thiosulfate, sulfur, sodium sesquicarbonate, magnesium sulfate, magnesium chloride, anhydrous silicic acid, metasilicic acid, boric acid, magnesium carbonate, magnesium hydroxide, dihydrogen Magnesium chloride, seawater dried product and the like can be mentioned.

Examples of the cleaning agent include fatty acid soaps, anionic surfactants such as sodium lauryl sulfate and alkyl ether carboxylates, and aliphatics.

As the emulsifier, a substance known as a cosmetic raw material can be used. Particularly preferred are sucrose fatty acid esters such as sucrose laurate, sucrose myristic ester, sucrose palmitate, sucrose stearate, sucrose oleate and sucrose erucate; tetraglycerin mono Stearic acid ester, tetraglycerin monooleic acid ester, tetraglycerin tristearic acid ester, hexaglycerin monolauric acid ester, hexaglycerin monomyristic acid ester, hexaglycerin monostearic acid ester, hexaglycerin tristearic acid ester, decaglycerin monolauric acid ester , Decaglycerin monomyristic acid ester, decaglycerin monostearic acid ester, decaglycerin distearic acid ester, decaglycerin Tristearic acid ester, decaglycerin pentastearic acid ester, decaglycerin monoisostearic acid ester, decaglycerin diisostearic acid ester, decaglycerin pentaisostearic acid ester, decaglycerin monooleic acid ester, decaglycerin trioleic acid ester, decaglycerin pentaolein Polyglycerin fatty acid esters such as acid esters; sorbitan branched fatty acid esters such as sorbitan monoisostearate, sorbitan sesquiisostearate, sorbitan triisostearate; soybean lecithin, egg yolk lecithin, hydrogenated soybean lecithin, hydrogenated egg yolk lecithin, and these lecithins Lysolecithin and / or hydrogenated lysolecithin converted into monoacyl form by enzymatic treatment, hydroxylation Lecithins such as hydroxy lecithin; monoisostearate, glyceryl sesquiisostearate, glyceryl glycerol branched fatty acid esters such diisostearate, glyceryl: can be exemplified, and can be used in combination of one or more.

In addition to the above, the cosmetic composition of the present invention includes, as necessary, other components such as lactose, milk, condensed milk, etc .; inorganic pigments such as titanium and talc; isopropylmethylphenol, chlorhexidine hydrochloride Bactericides such as chlorohexidine gluconate; Vitamins such as vitamin A, vitamin B1, vitamin B2, vitamin B6, vitamin P, CoQ10, CoQ9, CoQ8, thioctic acid, etc. and fluidization of coenzymes, silicic anhydride, synthetic aluminum silicate, etc. Agents: Pharmaceuticals, quasi-drugs, and tar-type pigments for cosmetics can be appropriately blended.

In addition to the components described above, various cosmetic components such as medicinal components for the purpose of beauty, facial treatment, skin treatment, and the like can be appropriately blended in the cosmetic of the present invention.
The above-mentioned medicinal ingredients include avocado extract, achacha extract, altea extract, arnica extract, apricot extract, apricot kernel extract, ginkgo biloba extract, fennel extract, turmeric extract, oolong tea extract, echinacea leaf extract, grass extract, barley extract, Dutch mustard extract , Orange extract, hydrolyzed wheat powder, hydrolyzed silk, chamomile extract, carrot extract, kawara mugi extract, calcade extract, kiwi extract, kina extract, cucumber extract, guanosine, kumazasa extract, walnut extract, grapefruit extract, clematis extract, yeast extract, Comfrey extract, Cowberry extract, Psycho extract, Saitai extract, Salvia extract, Soap extract, Sasa extract, Hawthorn extract, Shiitake extract, Giant Kiss, Shikon extract, Linden extract, Citrus extract, Ginger root extract, Birch extract, Horsetail extract, Honeysuckle extract, Horseshoe crab extract, Hawthorn extract, Horseshoe extract, Achillea millefolium extract, Horseshoe extract, Zenia mushroom extract, Assembled extract Extract, Thyme extract, Clove extract, Chigaya extract, Tomato extract, Natto extract, Novara extract, Hibiscus extract, Bacmond extract, Parsley extract, Parietalia extract, Toad extract, Bisabolol, Fukitan popo extract, Fukino honey, Tou extract, Bucurio extract, Butcher Bloom extract, grape extract, propolis, loofah extract, peppermint extract, bodaiju extract, hop extract, maize Extract, horse chestnut extract, skunk cabbage extract, Sapindus extract, peach leaf extract, cornflower extract, eucalyptus extract, apple extract, lettuce extract, Astragalus Saw extract, rose extract, rosemary extract, include the Roman chamomile extract, and the like.

Further, anti-inflammatory agents such as ε-aminocaproic acid, glycyrrhizic acid, dipotassium glycyrrhizinate, β-glycyrrhetinic acid, lysozyme chloride, guaiazulene, hydrocortisone, vitamin C, vitamin D, vitamin E, calcium pantothenate, biotin, nicotinamide , Vitamin E, blood circulation promoters such as vitamin E derivatives, vitamins such as β-carotene and carcinoid derivatives, active ingredients such as allantoin, diisopropylamine dichloroacetate, 4-aminomethylcyclohexanecarboxylic acid, carotenoids, flavonoids, tannins, lignans Antioxidants such as saponins, wound healing agents such as γ-oryzanol, proteolytic enzymes, cephalanthin, chili pepper, hinokitiol, garlic iodide extract, pyridoxine hydrochloride, nicotinic acid Nicotinic acid derivatives, acetyl pantothenyl ethyl ether, estradiol, ethinyl esteradiol, capronium chloride, diphenhydramine hydrochloride, camphor, nonyl acid vanillylamide, nonanoic acid vanillylamide, piroctone olamine, pentadecanoic acid glyceryl, l-menthol, menthol pyrrolidonecarboxylic acid Salt, mononitroguaiacol, urea, resorcin, γ-aminobutyric acid, benzethonium chloride, mexiletine hydrochloride, auxin, female hormone and female hormone-like substance, cantalis tincture, cyclosporine, analgesic, tranquilizer, antihypertensive, antibiotic In addition, antihistamines, antibacterial substances and the like can be arbitrarily blended.

The method for producing the cosmetic is not particularly limited, and a conventional production method can be used. In any step of the preparation of the cosmetic, a method for adding an extract of Habaldia mexicana as appropriate can be mentioned. .

In the cosmetics of the present invention, the amount of the extract of Habardia mexicana bark is preferably 0.001 to 10% by mass, more preferably 0.1 to 2% by mass based on the solid content, More preferably, it is 0.5-1 mass%. By setting it as the mixing | blending in the said range, the effect as an elastase inhibitor and an antioxidant can be obtained suitably.

EXAMPLES The present invention will be further described below with reference to examples, but the present invention is not limited to these examples. In addition, all compounding quantities represent the mass%.

(Method for preparing extract of Habardia mexicana bark)
To 500 g of Habardia Mexicana bark powder, add 15 L pure water, extract at low temperature (4 ° C) for 5 days, remove the precipitate by centrifugation (5000 rpm x 30 minutes), freeze-dry the supernatant, An aqueous extract (81 g) of Mexicana bark was obtained.

(Elastase inhibitory action)
Test Example 1 Measurement of Elastase Inhibitory Action An extract of Habardia mexicana bark was subjected to an elastase inhibitory action test as follows. That is, using a 96-well plate, 50 μL of a sample solution prepared with 0.1 mol / L Tris-HCl buffer (pH 8.0) and 50 μL of a 20 μg / mL porcine pancreatic elastase solution were mixed. Thereafter, 100 μL of 0.4514 mg / mL N-succinyl-Ala-Ala-Ala-p-nitroanilide prepared in the above buffer solution was added and reacted at 37 ° C. for 20 minutes. After completion of the reaction, absorbance at a wavelength of 415 nm was measured. Based on the following formula, the elastase inhibition rate (%) was calculated from the obtained data.

Elastase inhibition rate (%) = {1- (C−D) / (A−B)} × 100
In the formula, A represents “absorbance at a wavelength of 415 nm when only the enzyme is added”, B represents “absorbance at a wavelength of 415 nm when no sample is added and no enzyme is added”, and C is “wavelength when the sample is added and the enzyme is added” "Absorbance at 415 nm", and D represents "absorbance at a wavelength of 415 nm when a sample is added and no enzyme is added".
The results are shown in Table 1.

From the results shown in Table 1, it is clear that the extract of Habardia mexicana bark has excellent elastase inhibitory activity.

(antioxidant effect)
Test Example 2 Measurement of radical scavenging ability by DPPH method Antioxidant effect was evaluated by measuring radical scavenging ability of the extract of Habardia mexicana bark. Antioxidant activity was measured using 2,2-Diphenyl-1-picrylhydrazyl (manufactured by SIGMA, hereinafter referred to as DPPH) as a stable radical agent. DPPH is a compound (purple) having absorption at 517 nm, and the absorption at 517 nm disappears when radicals are eliminated. As an evaluation method, a solution of an extract of 80% ethanol and various concentrations of Habardia mexicana bark was added to 0.9 ml of 0.4 mM DPPH solution, and the decrease in absorbance at 517 nm was measured. Table 3 shows the measurement results of DPPH radical scavenging ability of the extract of Habardia mexicana bark.
The results are shown in Table 2.

(Prescription example)
Examples 1-6
Skin cosmetics (skin lotion, emulsion, cream, sheet pack, powder pack, bathing agent) containing the extract of the above-mentioned Habardia Mexicana bark were prepared with the following composition.

Example 1 (lotion)
[composition]
Raw material component amount (% by mass)
Ethanol 10.0
Polyoxyethylene hydrogenated castor oil (60 EO) 1.0
Glycerin 3.0
1,3-butylene glycol 2.0
Dipropylene glycol 3.0
Polyethylene glycol 1500 1.0
Phosphate 0.5
Methylparaben 0.1
Habardia Mexicana bark extract 0.1
Purified water remaining

Example 2 (milky lotion)
[composition]
Raw material component amount (% by mass)
(A component)
Stearic acid 1.0
Stearic acid glyceryl ester 2.0
Cetanol 1.0
Cholesterol 0.5
Vaseline 2.0
Squalene 5.0
Liquid paraffin 5.0
Silicone oil 1.0
Ptylparaben 0.1
Habardia Mexicana bark extract 0.5
(B component)
Acyl glutamate 1.0
Alkyl-modified carboxyvinyl polymer 0.2
Glycerin 2.0
Dipropylene glycol 3.0
Remaining amount of purified water totaling 100 [Preparation method]
The emulsion was prepared by adding the component B shown above to the component A and stirring.

Example 3 (cream)
[composition]
Raw material component amount (% by mass)
(A component)
Stearic acid 2.0
Stearic acid glycerin ester 2.0
Cetanol 3.0
Cholesterol 0.5
Vaseline 2.0
Squalene 5.0
Liquid paraffin 10.0
Silicone oil 1.0
Butylparaben 0.1
Habardia Mexicana Bark Extract 1.0
(B component)
Acyl glutamate 1.0
Carboxyvinyl polymer 0.15
Alkyl-modified carboxyvinyl polymer 0.15
Glycerin 5.0
Dipropylene glycol 3.0
Purified water remaining amount (preparation method)
Each of the A and B components shown above was dissolved by heating at 80 ° C. and then cooled to 30 ° C. while mixing and stirring to prepare each skin cream.

Example 4 (sheet-shaped pack)
[composition]
Raw material component amount (% by mass)
Habardia Mexicana Bark Extract 1.0
Sodium polyacrylate 2.0
Polyvinylpyrrolidone 0.5
Glycerin 25.0
1,3-butylene glycol 10.0
Paraben 0.1
Fragrance 0.1
L-Menthol 0.1
Impregnated nonwoven fabric 10.0
Purified water remaining

Example 5 (powdered pack)
[composition]
Raw material component amount (% by mass)
Habardia Mexicana Bark Extract 2.0
Sodium alginate 15.0
Talc 20.0
Citric acid 0.2
Sodium citrate 0.6
Paraben 0.1
Perfume Micro-grilled gypsum

Example 6 (bathing agent)
[composition]
Raw material component amount (% by mass)
Habardia Mexicana bark extract 0.5
Giant extract 0.1
Carrot extract 0.05
Chinpi powder 1.0
Macadamia nut oil 0.05
Hiba oil 0.1
1,3-butylene glycol 1.0
Xylitol 0.5
Polyoxyethylene sorbitan monolaurate 1.5
Anhydrous sodium sulfate

In Examples 1 to 6, lotions, emulsions, creams, sheet packs, powder packs, and bath additives containing an extract of Habardia Mexicana bark could be suitably prepared.

The obtained cosmetic was satisfactory in terms of feeling in use and stability.

The cosmetic of the present invention can be used as various skin cosmetics.

Claims (4)

A cosmetic comprising an extract of the bark of the leguminous leguminous genus Habardia and Mexicana bark. The cosmetic according to claim 1, which is an anti-aging cosmetic. An elastase inhibitor comprising, as an active ingredient, an extract of the bark of the leguminous leguminous genus Habardia, Habardia mexicana. Antioxidant containing an extract of the bark of the leguminous leguminous genus Habardia, Barbara mexicana