JP2013144651A - Keratin production promoter, food, skin external preparation, and hair restorer - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a new keratin production promoter which promotes keratin production, and can improve troubles of epidermal tissues such as a skin with insufficient strength, a brittle nail and a thin hair without stiffness caused by reduction of keratin and prevent weakening of the epidermal tissues, and to provide a new food, a skin external preparation and a hair restorer in which the new keratin production promoter is compounded.SOLUTION: The extract of Eleutherococcus senticosus is compounded as an active ingredient to promote keratin production in epidermal cells, and thereby, texture and luster of skin and a rough dry skin can be improved. By compounding a sweetener, fragrance, herbal medicine, nutritious supplement or the like as necessary in the keratin projection promoter, the obtained preparation can be ingested as foods. By compounding a cosmetic or a quasi drug, the promoter can be administered as a skin external preparation. By compounding the promoter in a hair restorer or a dye/manicure medical agent, conditions of hair or nails can be improved and maintained, and prevented from deteriorating.

Description

  The present invention relates to a keratin production promoter that promotes the production of skin horny layer keratin protein (hereinafter referred to as keratin), a food useful for preventing or alleviating phenomena such as skin, nails, hair deterioration and aging caused by keratin reduction, a skin external preparation, and It relates to hair restorer.

  Keratin is a major protein that constitutes the skin's surface layer, the stratum corneum, nails, and hair, and plays a very important role in the continuous generation and growth (metabolism) of these tissues.

  The production of keratin decreases due to stress, abnormal hormone balance, deterioration of metabolism due to aging, and the like. This produces keratin, nails, and hair with insufficient keratin components, leading to weakening of the skin tissue such as skin with insufficient strength, brittle nails, and firm, thin hair. In particular, insufficient skin strength causes external stimulation such as ultraviolet rays, a decrease in defense against infectious diseases, and an aging phenomenon. Brittle nails and fine hair are particularly problematic when performing manicure and hair coloring.

  Since keratin, which is a polymer, is difficult to be absorbed from the digestive tract and the skin, it is said that even if keratin itself is eaten or applied to the skin, it is difficult to increase the amount of keratin in the epidermis tissue. Therefore, by activating the natural keratin production ability inherent in the epidermal tissue and promoting keratin production, it prevents the weakening of the epidermal tissue due to keratin deficiency and prevents problems such as inflammation, infection, and aging Such keratin production promoters, foods, skin external preparations and the like are demanded.

  Japanese Patent Application Laid-Open No. 11-158053 discloses that cosmetics formulated with an extract of Argiaceae plants have effects of moisturizing the skin, promoting blood circulation, and improving skin diseases. Japanese Patent Laid-Open No. 2001-181147 discloses that a hair cosmetic blended with an extract of Ezokogi prevents hair damage and prevents cracking and fluffing by ultraviolet absorption.

Japanese Patent Laid-Open No. 11-158053 JP 2001-181147 A

  In the present invention, a novel keratin production promoter that promotes keratin production and can prevent and improve skin weakness due to keratin reduction and weakening of epidermal tissues such as brittle nails and firm, thin hair, An object is to provide a novel food, an external preparation for skin, and a hair restorer containing the same. It is another object of the present invention to provide a keratin production promoter that does not significantly affect the differentiation of epidermal cells and therefore increases only the keratin production ability while maintaining the differentiation ability of stem cells.

  In order to achieve the above-mentioned object, the present inventors have conducted intensive research to obtain a substance that exhibits a keratin production promoting effect from among substances having excellent safety. It has been found that an extract of Ekogigi, which has been known as a substance having the above, has a remarkable keratin production promoting effect, and has completed the present invention.

  Siberian ginseng (scientific name Acanthopanex Senticosusu Harms) is a deciduous shrub of the family Araceae and is known as a medicinal plant with nourishing, tonic and anti-stress effects. The sorghum extract used in the present invention is extracted from stalks, roots, leaves and the like of sorghum with water or an organic solvent. The organic solvent used for extraction may be a polar solvent or a nonpolar solvent, and the extraction method is not particularly limited.

  The blending amount of the sorghum extract in the keratin production promoter of the present invention can be adjusted according to the dosage form and application, but it is preferably diluted from the viewpoint of stability and safety. According to the inventor's experiment, even when the sorghum extract is used alone, it enhances the keratin production function of epidermal cells and exhibits a good keratin production promoting effect. Even a relatively low concentration of Ezocogi extract is effective, so it can be safely blended into foods, external preparations for skin, hair restorers and the like.

  The food containing the keratin production promoter of the present invention contains, in addition to the essential component Ezoukogi extract, if necessary, a sweetener, an antiseptic, an antioxidant, and a fragrance within a range that does not impair the effects of the present invention. Herbal medicines and nutritional supplements can be added. In addition, the form of food containing the keratin production promoter of the present invention can take various forms that can be taken orally, such as powders, powders, granules, tablets, capsules, etc. You can also.

  The external preparation for skin containing the keratin production promoter of the present invention includes cosmetics, quasi-drugs and pharmaceuticals as long as they do not impair the effects of the present invention, if necessary, in addition to the essential component sorghum extract For example, various components generally used in the field, aqueous components, humectants, thickeners, ultraviolet absorbers, ultraviolet scattering agents, preservatives, antioxidants, fragrances, colorants, drugs, crude drugs and the like can be blended. The dosage form of the external preparation for skin containing the keratin production promoter of the present invention is arbitrary, for example, a solubilizing system such as lotion, an emulsifying system such as an emulsion or cream, an ointment, a powder dispersion system, or a water-oil. A dosage form such as a two-layer system or a water-oil-powder three-layer system may be used.

  Since the keratin production promoter of the present invention increases keratin, which is the main component of hair and nails, it is effectively prevented from deteriorating hair, nails and the like by blending with hair restorers and hair coloring and nail polish agents. be able to. These agents can be blended with other components necessary for the function of the preparation within a range not impairing the effects of the present invention.

  Hereinafter, the present invention will be described in detail with reference to experimental examples. This description is intended to clarify the method of carrying out the present invention, and the illustrated methods, numerical values, and the like do not limit the invention.

Test of cytokeratin 1 production-inducing ability of Ezoukogi extract <Sample>
As a sample, Ezokogi extract (ratio of crude drug equivalent of about 20: 1) manufactured by Nippon Powder Co., Ltd. was used.
<Test method>
(Examination subject)
Human epidermis-derived cell line HaCaT cells were used as a cell evaluation system for cytokeratin 1 production ability. HaCaT cells correspond to cells in the basal layer in the human epidermis, and have the property of exhibiting a trait that causes cell growth to stop and differentiate into spinal and subsequent cells by changing the culture conditions. Cytokeratin 1 is a main component that forms the horny layer of the epidermis, is hardly produced in the basal layer, and is produced from the spinous layer onward. Therefore, it is generally treated as an index (differentiation marker) in which cells in the basal layer are differentiated.

(Test method)
Using the above-mentioned HaCaT cell line with a basic culture solution (Dulbecco's modified Eagle's medium, fetal bovine serum added as a culture auxiliary factor at a final concentration of 10%) in a 12-well plastic plate, 1.5 × 10 ⁴ cells per well (of the culture area) Culture density occupying about 10%) was seeded and cultured at 37 ° C. in a 5% CO ₂ incubator.

  A culture solution (hereinafter abbreviated as ESE culture solution) obtained by adding 1/100 volume of Ezocogi extract (ESE) to the basic culture solution is prepared, and after exchange on these culture solutions on the first day after seeding, on the fourth day. The culture was continued by exchanging the two ESE culture solutions and the basic culture solution only for the first day, and two cell culture groups, an ESE addition group and an ESE removal group, were constructed. Further, after 3 days, the cells were collected, and the presence or absence of cytokeratin 1 in the cells and production changes were analyzed by Western blotting. As a control group, cells cultured in a culture medium supplemented with 1/100 volume of 30% ethanol (final concentration 0.3%) were used. The detection results of cytokeratin 1 are shown in FIGS.

(Test results)
As a result of the above test, it was found that cytokeratin 1 was produced much more than usual in the ESE addition group. Cytokeratin 1 was hardly produced in the cells of the control group. In addition, when the Ekougiri treatment was interrupted, the production amount of cytokeratin 1 rapidly decreased to the same level as that of the control group. From the observation result with a microscope, the cell morphology (cell differentiation) was almost the same as the control group. These results indicate that the increase in the production amount of cytokeratin 1 is not due to the differentiation of stem cells, but is due to the direct increase in the ability to produce cytokeratin 1 in each epidermal cell.

  From the above, it has been clarified that the product of the present invention has an effect of directly inducing the production of cytokeratin 1 directly without affecting the differentiation of epidermal cells of human skin.

On the other hand, the state and physical properties of keratin in the epidermis are also known to be affected by changes in the amount of amino acids such as NMF (Natural Moisturizing Factor). For example, NMF is treated with acetone / ether / water. In the rough skin model forcibly removed, keratin aggregation accompanied by skin softness reduction is observed. Aggregated keratin is not redispersed simply by applying water, but affects the water retention capacity of the keratin (Non-patent Document 1). Cytokeratin 1 is expressed in the spinous layer of the epidermis and affects the fineness of skin texture in addition to keratinization and barrier function.
“Fragrance Journal”, September 2004, p. 53

  Therefore, we apply food containing elephant extract and topical skin preparation to subjects with rough skin, and change the skin stratum corneum due to its cytokeratin 1 production promoting action, especially against dryness, roughness, roughness, texture and rough skin. The improvement effect was tested.

Food production example 1 (1) Manufacture of drink: A test product containing 0.1% Ezokogi extract was prepared as shown in Table 1, and a practical use test was conducted for one month. Ezoukogi extract is extracted with 30% ethanol aqueous solution of Ezocogi root, and the drug substance conversion value is 14: 1 (solid content is about 35%), and contains 50 mg of drug substance conversion per 10 mL of test product.

  The panelists were 14 females in their late 20s to 50s who had marked symptoms such as dry skin, roughness, roughness, and rough skin. These compositions are used once a day at 10 mL for 2 weeks to 1 month, and the skin condition was “improved”, “slightly improved”, “no change” before and after the use test. ”Was evaluated in three stages. The test results are shown in Table 2 in terms of the number of panelists that obtained each evaluation.

  As shown in Table 2, it was confirmed that the test product had an improvement effect from the 2nd week of continuous use on the roughness and roughness of the skin, and that the effect increased as it was used continuously for dryness and rough skin. None of the panelists showed any allergic reaction or improvement.

Skin external preparation production example (1) Manufacture of essence: A essence having the composition shown in Table 3 was prepared using an extract of Ekogigi extracted by a conventional method.

  The method for producing the Ezoukogi extract was to add about 5 L of 30 v / v% ethanol to 1 kg of shredded Rhizome rhizome, gently heat extract and then filter. The residue was extracted twice with about 5 L of 30 v / v% ethanol in the same manner, and all the extracts were combined and concentrated under reduced pressure at 50 ° C. to obtain about 500 mL of the concentrated solution. An appropriate amount of absolute ethanol was added to this solution to adjust the ethanol concentration to 30 v / v%. This was left to stand for 7 to 10 days in a cool place and ripened, and the sediment and precipitate produced in this step were removed by filtration, and at the same time, 30 v / v% ethanol was added to obtain 500 mL of product.

As a panelist, 42 women in their twenties and over were asked to apply an appropriate amount of sorghum-containing serum once or twice a day for 3 months to evaluate the improvement effect on dryness, texture, and rough skin (actual test) Is a comparison with no application). The number of panelists that obtained each evaluation was as shown in FIGS.

  Next, as a panelist, long-term continuous use on the side of the neck of a 27-year-old woman, the form was regularly observed, and the results shown in FIGS. 6 and 7 were obtained. The texture index is a numerical value of the uniformity of the texture of the skin appearance. The uniform value (maximum value) is 1, and the value decreases as it is disturbed. The rate of change in texture was [left +1.3, right -0.23 (18 months)] in the neck, and [cold] was [left +0.93, right -0.36 (14 months)].

Moreover, the moisture content of the neck skin of 6 persons as a panelist was measured, and compared with the user and the non-continuous user of the cosmetic liquid containing an elephant extract, or a continuous part and a non-continuous part, Table 5, 6, and FIG. 9 results were obtained.

  In all three subjects (KT, TS, H) who continued to use only one side of the neck, the stratum corneum water content increased significantly in a continuous site-specific manner. (Table 5 and FIG. 8). The neck of the subject (K) who did not use the preparation had the same amount of keratinous water on both the left and right sides, and no significant difference was observed (Table 6 and FIG. 9).

  Among the subjects who used the entire neck, 2 out of 4 subjects (TH, MY) showed a significant increase over time. The period of continuous use for the two patients who showed a significant difference was 5 to 10 months. When used continuously during the winter, the keratin moisture content increased by 1.4 to 1.8 times, and after prolonged use by 1.2 to 2.7 times (continuous use in the neck). That is, it is considered that the continuous water use increases the moisture retention ability of the skin, thereby preventing dryness and improving the rough skin and the texture disorder caused by the dryness.

Example of preparation for external preparation for skin (2) Production of pack preparation: The ingredients in Table 7A were dissolved by heating, and the ingredients of B that were heated and mixed were added with stirring. After cooling, an appropriate amount of fragrance was added and allowed to stand. A pack having the composition described was produced.

Skin external preparation production example (3) Manufacture of cream: A cream having the composition shown in Table 8 was prepared using an extract of Ezokogi extracted by a conventional method.

Production of hair restorer: A hair restorer having the composition described in Table 9 and Table 10 was produced using Ezoukogi extract extracted by a conventional method.

  The keratin production promoter of the present invention has the effect of dramatically increasing the keratin production ability in human epidermal cells, and it is known that the sorghum extract can be administered as a food, a skin external preparation, etc. It can be expected to have an effect of preventing or improving the deterioration of the protective ability against external stimuli such as ultraviolet rays due to weakening of the epidermis tissue such as skin lacking strength due to the decrease, brittle nails, firm and thin hair, and skin aging. Therefore, it is useful in the production of foods, cosmetics, hair cosmetics and the like.

It is a photograph which shows the detection result of the cytokeratin 1 of the control group, ESE addition group, and ESE removal group by electrophoresis. It is the graph which showed the production amount of cytokeratin 1 in the same test by the relative value which set the 0th day of the control group to 1. It is a graph which shows the improvement effect with respect to the rough skin of the cosmetic liquid which concerns on Example 2. FIG. It is a graph which shows the improvement effect with respect to drying of the same cosmetic liquid. It is a graph which shows the improvement effect with respect to the texture of the skin of the same cosmetic liquid. It is a graph which shows the change (neck) of the texture of the skin at the time of using the same cosmetic liquid continuously. It is a graph which shows the change (decorte) of the texture of the skin at the time of using the same cosmetic liquid continuously. It is a graph which shows the change of the amount of stratum corneum when the same cosmetic liquid is used continuously. It is a graph which shows the change of the amount of keratin moisture of the test subject who did not use a formulation.

Claims (4)

A keratin production promoter containing Ezoukogi extract as an active ingredient. A food containing the keratin production promoter according to claim 1. A skin external preparation containing the keratin production promoter according to claim 1. A hair restorer containing the keratin production promoter according to claim 1.