JP2013067585A - Deodorant composition - Google Patents

Abstract

PROBLEM TO BE SOLVED: To satisfy requirements that it is desirable to find, in particular, a substance that has an antibacterial effect on Fusobacterium nucleatum and Porphyromonas gingivalis and a metabolic inhibitory effect on Fusobacterium nucleatum and Porphyromonas gingivalis, in order to inhibit production of hydrogen sulfide and methyl mercaptan in the saliva for bad breath prevention, and further that it is desirable that such a substance having an effect of inhibiting bad breath causes no safety issue when taken into the body.SOLUTION: A composition for oral cavity includes as the active ingredient an extract of leaves or stems of Angelica keiskei. An exudate from Angelica keiskei may be used as the extract of Angelica keiskei.

Description

  In recent years, interest in bad breath has increased. As a causative substance of bad breath, volatile sulfur compounds (VSC) such as hydrogen sulfide and methyl mercaptan are known. Hydrogen sulfide is detected more in the oral cavity, but methyl mercaptan has a strong odor even in small amounts. Therefore, it is important to suppress the production of these substances to prevent bad breath.

  VSCs are produced by the metabolism of anaerobic bacteria in the oral cavity using shed mucosal epithelial cells, proteins in serum, food residues and sulfur-containing amino acids in food as a substrate. As such anaerobic bacteria, Fusobacterium nucleatum and Porphyromonas gingivalis are known, and these bacteria produce hydrogen sulfide from cysteine by hydrogen sulfide-producing enzyme and methyl mercaptan from methionine by methioninase.

  It is important to suppress the production of hydrogen sulfide and methyl mercaptan in saliva for the suppression of bad breath, which includes antibacterial action against Fusobacterium nucleatum and Porphyromonas gingivalis, metabolic inhibition action against Fusobacterium nucleatum and Porphyromonas gingivalis, and hydrogen sulfide production It is thought that it can be achieved by enzyme inhibitory action on enzymes and methioninase, and direct action on hydrogen sulfide and methyl mercaptan (decomposition of these substances, etc.). Therefore, discovery of substances having these actions is desired. Moreover, as a substance which has such a bad breath suppression effect, since the effect | action when ingested in an oral cavity is desired, it is desirable that it is a substance which does not have a safety problem even if it ingests in the body.

As a result of intensive studies in view of the above problems, the inventor found that the extract of leaves, stems, or roots of tomorrow had a bad breath suppression effect, and thus completed the present invention.
That is, this invention relates to the composition for oral cavity which uses the extract of the leaf, stem, or root of tomorrow as an active ingredient.

  According to the composition for oral cavity of the present invention, production of hydrogen sulfide and methyl mercaptan in saliva can be suppressed, and bad breath can be prevented.

The graph which shows the quantity of the hydrogen sulfide produced in Example 1 (by the saliva culture method). The graph which shows the quantity of the methyl mercaptan produced in Example 1 (by the saliva culture method). The graph which shows the quantity of the hydrogen sulfide produced in Example 2 (by a fungal metabolism inhibition test).

The present invention relates to a composition for oral cavity containing an extract of leaves, stems or roots of tomorrow as an active ingredient.
The tomorrow is a perennial herb belonging to the genus Cericidae, and its scientific name is Angelica keiskei. Tomorrow contains a large amount of yellow matter in the roots, stems, and leaves, and yellow juice exudes from the cut surface. In the present invention, the toasted leaf extract includes this toasted leaf yellow juice. The main components of this yellow juice are chalcones. Among them, two types, “xanthoangelol” and “4-hydroxyderricin”, are contained in particular.

  As described in detail in the Examples, the present inventors have found that tomorrow leaf extract has an effect of suppressing the production of hydrogen sulfide and methyl mercaptan, which are malodorous substances in saliva. Has been found to have antimetabolic and antibacterial effects on Fusobacterium nucleatum.

The composition for oral cavity of the present invention preferably contains a leaf extract prepared as an active ingredient in a proportion of 50% by weight to 0.05% by weight, and more preferably in a proportion of 10% by weight to 0.1% by weight.
As extraction conditions, extraction can be performed at any one of high temperature, room temperature, and low temperature, but it is preferable to perform reflux extraction with ethanol or the like for about 1 hour. The obtained extract may be filtered and the extraction solvent may be distilled off, followed by concentration or lyophilization under reduced pressure. In addition, those obtained by fractionating and purifying these extracts using an organic solvent, column chromatography, or the like can also be used.
In addition, the oral composition of the present invention preferably contains leaf yellow juice as an active ingredient in a proportion of 50 wt% to 0.05 wt%, more preferably in a proportion of 10 wt% to 0.1 wt%. To do.
The oral composition of the present invention preferably contains chalcones derived from tofu as an active ingredient in a proportion of 5% by weight to 0.005% by weight, more preferably a proportion of 1% by weight to 0.01% by weight. Contains.

According to the composition for oral cavity of the present invention, production of hydrogen sulfide and methyl mercaptan in saliva can be suppressed, and bad breath can be prevented. Moreover, the composition for oral cavity of the present invention has a metabolic inhibitory effect and antibacterial action against Fusobacterium nucleatum.
In addition, since the deodorant composition of the present invention is highly safe, for example, a deodorant composition such as a mouthwash, a toothpaste, a deodorant spray, etc. , Snacks and other confectionery, ice cream, sorbet, frozen confectionery such as ice confectionery, beverages, bread, hot cakes, dairy products, ham, sausage and other livestock products, sea bream, chikuwa and other fish products, side dishes, pudding, soup and It can be blended into foods and drinks such as jam and used on a daily basis.

In addition, it is not limited above regarding components other than tomorrow leaf extract, The present invention may optionally contain any known components.
The composition for oral cavity containing the tofu extract of the present invention can be produced by any method known in each technical field. In the production process, the tomato leaf extract may be added by any known method.
In addition, the composition for oral cavity containing the tofu extract of the present invention can be used not only for humans but also for animals other than humans (hereinafter abbreviated as non-human animals). Examples of non-human animals include non-human animals such as mammals, reptiles, amphibians and fish.
In addition, since the present invention uses tomorrow, which has been conventionally used as a food, there is no problem with its safety even if it is taken into the body.

  The present invention will be further described below with reference to examples, but these examples do not limit the scope of the present invention.

Samples The following samples used in the following examples were all obtained from Nippon Biological Science Co., Ltd.
・ Toast leaf yellow juice: Chalcone content 10.53% (xanthoangelol 66.3 mg / g, 4-hydroxy derricin 39.0 mg / g), sap collected from the stem of tomorrow leaf ・ Tomorrow leaf polyphenol CHALSAP-P8 (hereinafter, simply CHALSAP-P8): Tomorrow extract 20-30% (total chalcone content 8% or more), branched cyclodextrin 80-70%. A powder formulation prepared by mixing dried tofu and branched cyclodextrin and then freeze-drying.
・ Branched cyclodextrin: used for CHALSAP-P8 ・ 4-Hydroxyderricin ・ Xanthoangelol

[Example 1] Saliva culture test
1) Saliva collection Each panelist banned eating, drinking and smoking 0.5-1.0 hours before saliva collection. The parafilm was chewed to collect saliva, and after sufficient vortexing, equal amounts of 3 people's saliva were mixed.
2) Test method 1.0 ml of mixed saliva and 1.0 ml of test sample solution (1% ethanol) were placed in a capped test tube and cultured anaerobically at 37 ° C for about 20 hours. Then, after ice-cooling and shaking at 37 ° C. for 20 minutes, the headspace gas was injected into a gas chromatograph with an FPD detector, and the amounts of hydrogen sulfide and methyl mercaptan were calculated from the obtained peak heights. Test samples included CHALSAP-P8 (0.2 mg / ml and 0.1 mg / ml), xanthoangelol (0.2 mg / ml and 0.1 mg / ml), 4-hydroxyderricin (0.2 mg / ml and 0.1 mg / ml). ) And 1% ethanol was used as a control.
As a control, the same experiment was conducted without adding the test sample, and the amount of hydrogen sulfide and methyl mercaptan (M _S ) when the sample was added and the amount of hydrogen sulfide and methyl mercaptan (M _B ) without addition were determined. The inhibition rate was calculated by the formula.
Inhibition rate _{(%) = (M B -} M S) / M B × 100
3) Results
Produced in CHALSAP-P8 (0.2 mg / ml and 0.1 mg / ml), xanthoangelol (0.2 mg / ml and 0.1 mg / ml), 4-hydroxy derricin (0.2 mg / ml and 0.1 mg / ml) Fig. 1 shows the amount of hydrogen sulfide and Fig. 2 shows the amount of methyl mercaptan. In addition, Table 1 shows the respective inhibition rates. It was confirmed that CHALSAP-P8, xanthoangelol, and 4-hydroxyderricin decreased the amount of methyl mercaptan and hydrogen sulfide in saliva in a concentration-dependent manner, thereby inhibiting the production of these substances.

[Example 2] Bacterial metabolism inhibition test
1) Test method
Fusobacterium nucleatum JCM8532 was cultured and then centrifuged, and the cells were suspended in PBS to give OD ₅₅₀ = 0.3. Add 1 ml of bacterial suspension to 1 ml of PBS containing 2 mM of cysteine and test sample, react for 90 minutes at 37 ° C under anaerobic conditions, and then inject 50 μl of headspace into the gas chromatograph with FPD detector. The amount of hydrogen sulfide was determined from the obtained peak height. As a test sample, 1.0 mg / ml toasted leaf yellow juice was used.
As a control, the same experiment was performed without adding the test sample, and the inhibition rate was calculated in the same manner as in Example 1.
2) Results Figure 3 shows the amount of hydrogen sulfide produced. The hydrogen sulfide production inhibition rate at this time was 30%. It was confirmed that tofu leaf yellow juice has the effect of inhibiting the metabolism of Fusobacterium nucleatum.

[Example 3] Antibacterial test
1) Test method
After culturing Fusobacterium nucleatum JCM8532, it was diluted in TSB medium having a double concentration to obtain a test bacterial solution. A test sample was prepared in a 2-fold serial dilution series in a 96-well plate, a test bacterial solution was added thereto, and anaerobic culture was performed. Four days later, visual judgment and measurement of OD ₆₆₀ value were performed with a microplate reader. In the test sample, the antibacterial action is observed by diluting the amount of toast leaf yellow juice, CHALSAP-P8, branched cyclodextrin, 4-hydroxy derricin and xanthoangelol from 1000ppm to 15.625ppm respectively. This was determined by visual inspection and absorbance determination. At that time, the concentration at which the OD ₆₆₀ value was comparable to that of the control and the growth of the bacteria was not visually observed was defined as MIC (minimum growth inhibitory concentration).
In the same manner, the antibacterial test of CHALSAP-P8 against Porphyromonas gingivalis W83, Porphyromonas gingivalis FDC381, and Porphyromonas gingivalis ATCC33277 was repeated 3 times.
2) Results Antibacterial effects against Fusobacterium nucleatum JCM8532 were confirmed except for branched cyclodextrins. The MIC (minimum growth inhibitory concentration) at that time was 125 ppm for tofu and 250 ppm for 4-hydroxyderricin, xanthoangelol and CHALSAP-P8.
The antibacterial effect of CHALSAP-P8 against Porphyromonas gingivalis was also confirmed. The MIC is 31.25 to 125 ppm for Porphyromonas gingivalis W83 (125 ppm for the first time, 62.5 ppm for the second time, 31.25 ppm for the third time), and 62.5 to 125 ppm for Porphyromonas gingivalis FDC381 (125 ppm for the first time, 62.5 for the second time) ppm, 32.5 ppm for Porphyromonas gingivalis ATCC33277 (125 ppm for the first, 31.25 ppm for the second, 31.25 ppm for the third).

[Example 4] Enzyme inhibition test
1) Hydrogen sulfide producing enzyme inhibition test
A mixture of Fusobacterium nucleatum JCM8532 cell disruption solution (0.3 mg protein / ml), 30 mM L-cysteine, 50 μM pyridoxal phosphate, 0.2 M phosphate buffer (pH 7.5) and the sample was shaken at 37 ° C. After 1 hour, 0.5 ml of 6% perchloric acid was added to stop the reaction. After centrifugation (3,000 × g, 10 minutes, 4 ° C.), the supernatant was recovered. To 0.4 ml of the supernatant, 1.2 ml of MBTH reaction solution was added and reacted at 50 ° C. for 30 minutes. The reaction solution was diluted 3-fold with a sodium acetate buffer, the absorbance was measured at 335 nm, and the amount of pyruvic acid was calculated. Tomato leaf yellow juice (concentration: 1.0 mg / ml) was used as a test sample.
As a control, the same experiment was performed without adding the test sample, and the inhibition rate was calculated in the same manner as in Example 1.
2) Methioninase inhibition test
A mixture of Fusobacterium nucleatum JCM8532 cell disruption solution (0.3 mg protein / ml), 30 mM L-methionine, 50 μM pyridoxal phosphate, 0.2 M phosphate buffer (pH 7.5) and the sample was shaken at 37 ° C. After 1 hour, 0.5 ml of 6% perchloric acid was added to stop the reaction. After centrifugation (3,000 × g, 10 minutes, 4 ° C.), the supernatant was recovered. To 0.4 ml of the supernatant, 1.2 ml of MBTH reaction solution was added and reacted at 50 ° C. for 30 minutes. The reaction solution was diluted 3-fold with sodium acetate buffer, and the absorbance was measured at 335 nm to calculate the amount of α-ketobutyric acid. Tomato leaf yellow juice (concentration: 1.0 mg / ml) was used as a test sample.

As a control, the same experiment was performed without adding the test sample, and the inhibition rate was calculated in the same manner as in Example 1.
3) Results The inhibition rate of the hydrogen sulfide-producing enzyme inhibition test was 8%, and the inhibition rate of the methioninase inhibition test was 9%.

[Example 5]
Chewing gum, candy, gummy jelly, troche, dentifrice and mouthwash were produced using the tomato leaf extract according to the following formulation.

(Example 5-1)
Chewing gum formula xylitol 45.0% by weight
Maltitol 33.0
Gum base 14.0
Fragrance 1.0
Tomorrow yellow juice 0.2
Other remaining
100.0

(Example 5-2)
Candy prescription sugar 50.0% by weight
Mizuame 33.0
Citric acid 14.0
Fragrance 1.0
Tomorrow yellow juice 0.5
Other remaining
100.0

(Example 5-3)
Gummy jelly prescription gelatin 67.0% by weight
Reduced water candy 25.0
Vegetable oil and fat 5.0
Malic acid 2.0
Fragrance 0.5
Tomorrow yellow juice 0.5
100.0

(Example 5-4)
Troche prescription sugar 76.0% by weight
Glucose 19.0
Sucrose fatty acid ester 0.2
Fragrance 0.2
Tomorrow extract 0.5
Water remaining
100.0

(Example 5-5)
Dentifrice prescription calcium carbonate 50.0% by weight
Glycerin 20.0
Carboxymethylcellulose 2.0
Sodium lauryl sulfate 2.0
Fragrance 1.0
Tomorrow extract 0.5
Saccharin 0.1
Chlorhexidine 0.01
Water remaining
100.0

(Example 5-6)
Prescription of mouthwash glycerin 10.0% by weight
Sodium lauryl sulfate 1.5
Fragrance 0.8
Tomorrow extract 0.5
Saccharin sodium 0.2
Water remaining
100.0

Claims (5)

  An oral composition comprising an extract from leaves, stems or roots of tomorrow as an active ingredient.   The composition for oral cavity according to claim 1, wherein the extract is toasted leaf yellow juice.   The composition for oral cavity according to claim 1 or 2, wherein the extract contains chalcones.   The composition for oral cavity according to any one of claims 1 to 3, which has an antibacterial action against Fusobacterium nucleatum or Porphyromonas gingivalis.   The composition for oral cavity of any one of Claims 1-4 which suppresses production of hydrogen sulfide or methyl mercaptan by Fusobacterium nucleatum or Porphyromonas gingivalis.

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