JP2012530123A5 - - Google Patents
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- Publication number
- JP2012530123A5 JP2012530123A5 JP2012515567A JP2012515567A JP2012530123A5 JP 2012530123 A5 JP2012530123 A5 JP 2012530123A5 JP 2012515567 A JP2012515567 A JP 2012515567A JP 2012515567 A JP2012515567 A JP 2012515567A JP 2012530123 A5 JP2012530123 A5 JP 2012530123A5
- Authority
- JP
- Japan
- Prior art keywords
- buffer solution
- stress protein
- target
- buffer
- target stress
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 102000002812 Heat-Shock Proteins Human genes 0.000 claims 13
- 108010004889 Heat-Shock Proteins Proteins 0.000 claims 13
- 238000000034 method Methods 0.000 claims 13
- 239000007853 buffer solution Substances 0.000 claims 9
- 239000000872 buffer Substances 0.000 claims 7
- 150000001768 cations Chemical class 0.000 claims 5
- 239000000203 mixture Substances 0.000 claims 5
- ZKHQWZAMYRWXGA-KQYNXXCUSA-J ATP(4-) Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)[C@H]1O ZKHQWZAMYRWXGA-KQYNXXCUSA-J 0.000 claims 4
- ZKHQWZAMYRWXGA-UHFFFAOYSA-N Adenosine triphosphate Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)C(O)C1O ZKHQWZAMYRWXGA-UHFFFAOYSA-N 0.000 claims 4
- XTWYTFMLZFPYCI-KQYNXXCUSA-N 5'-adenylphosphoric acid Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP(O)(=O)OP(O)(O)=O)[C@@H](O)[C@H]1O XTWYTFMLZFPYCI-KQYNXXCUSA-N 0.000 claims 3
- XTWYTFMLZFPYCI-UHFFFAOYSA-N Adenosine diphosphate Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(COP(O)(=O)OP(O)(O)=O)C(O)C1O XTWYTFMLZFPYCI-UHFFFAOYSA-N 0.000 claims 3
- 239000013592 cell lysate Substances 0.000 claims 3
- 108091006112 ATPases Proteins 0.000 claims 2
- 102000057290 Adenosine Triphosphatases Human genes 0.000 claims 2
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims 2
- 238000005342 ion exchange Methods 0.000 claims 2
- 150000002696 manganese Chemical class 0.000 claims 2
- 229920001184 polypeptide Polymers 0.000 claims 2
- 239000011591 potassium Substances 0.000 claims 2
- 229910052700 potassium Inorganic materials 0.000 claims 2
- 102000004196 processed proteins & peptides Human genes 0.000 claims 2
- 108090000765 processed proteins & peptides Proteins 0.000 claims 2
- 238000000746 purification Methods 0.000 claims 2
- 239000004094 surface-active agent Substances 0.000 claims 2
- 229960005486 vaccine Drugs 0.000 claims 2
- JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 claims 1
- 208000035473 Communicable disease Diseases 0.000 claims 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 claims 1
- 102000007474 Multiprotein Complexes Human genes 0.000 claims 1
- 108010085220 Multiprotein Complexes Proteins 0.000 claims 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims 1
- 239000004202 carbamide Substances 0.000 claims 1
- 208000015181 infectious disease Diseases 0.000 claims 1
- 239000006166 lysate Substances 0.000 claims 1
- 239000011777 magnesium Substances 0.000 claims 1
- 229910052749 magnesium Inorganic materials 0.000 claims 1
- 159000000003 magnesium salts Chemical class 0.000 claims 1
- 230000003211 malignant effect Effects 0.000 claims 1
- 244000052769 pathogen Species 0.000 claims 1
- XAEFZNCEHLXOMS-UHFFFAOYSA-M potassium benzoate Chemical compound [K+].[O-]C(=O)C1=CC=CC=C1 XAEFZNCEHLXOMS-UHFFFAOYSA-M 0.000 claims 1
- 159000000001 potassium salts Chemical class 0.000 claims 1
- 238000002360 preparation method Methods 0.000 claims 1
- 102000004169 proteins and genes Human genes 0.000 claims 1
- 108090000623 proteins and genes Proteins 0.000 claims 1
- 150000003839 salts Chemical class 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB0910591.7A GB0910591D0 (en) | 2009-06-19 | 2009-06-19 | Method for the purification of protein complexes |
| GB0910591.7 | 2009-06-19 | ||
| PCT/GB2010/051023 WO2010146401A1 (en) | 2009-06-19 | 2010-06-21 | Method for the purification of protein complexes |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2012530123A JP2012530123A (ja) | 2012-11-29 |
| JP2012530123A5 true JP2012530123A5 (enExample) | 2013-08-08 |
| JP5778666B2 JP5778666B2 (ja) | 2015-09-16 |
Family
ID=40941082
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2012515567A Expired - Fee Related JP5778666B2 (ja) | 2009-06-19 | 2010-06-21 | タンパク質複合体の精製方法 |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US10040820B2 (enExample) |
| EP (1) | EP2442825B1 (enExample) |
| JP (1) | JP5778666B2 (enExample) |
| CN (1) | CN102458460B (enExample) |
| AU (1) | AU2010261505B2 (enExample) |
| GB (1) | GB0910591D0 (enExample) |
| IN (1) | IN2012DN00250A (enExample) |
| WO (1) | WO2010146401A1 (enExample) |
| ZA (1) | ZA201200420B (enExample) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SG10201508401TA (en) * | 2010-10-11 | 2015-11-27 | Abbvie Bahamas Ltd | Processes for purification of proteins |
| GB201104897D0 (en) | 2011-03-23 | 2011-05-04 | Immunobiology Ltd | Method for the production of protein complexes and vaccine compositions comprising the same |
| CN103687616A (zh) * | 2011-07-21 | 2014-03-26 | 生物技术工具公司 | Dnak制剂 |
| CA3066247A1 (en) | 2017-06-07 | 2018-12-13 | University Of Maryland, Baltimore County | Factory-on-a-chip for production of biologically derived medicines/biopharmaceuticals/biologics/ biotherapeutics |
| KR102270048B1 (ko) * | 2020-07-10 | 2021-06-28 | 주식회사 녹십자 | 바리셀라 조스터 바이러스 표면 단백질 항원의 생산 방법 |
Family Cites Families (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5750119A (en) * | 1994-01-13 | 1998-05-12 | Mount Sinai School Of Medicine Of The City University Of New York | Immunotherapeutic stress protein-peptide complexes against cancer |
| US5997873A (en) | 1994-01-13 | 1999-12-07 | Mount Sinai School Of Medicine Of The City University Of New York | Method of preparation of heat shock protein 70-peptide complexes |
| US5961979A (en) | 1994-03-16 | 1999-10-05 | Mount Sinai School Of Medicine Of The City University Of New York | Stress protein-peptide complexes as prophylactic and therapeutic vaccines against intracellular pathogens |
| US5837251A (en) | 1995-09-13 | 1998-11-17 | Fordham University | Compositions and methods using complexes of heat shock proteins and antigenic molecules for the treatment and prevention of neoplastic diseases |
| EP0851765A4 (en) * | 1995-09-13 | 2000-01-19 | Univ Fordham | THERAPEUTIC AND PROPHYLACTIC PROCESSES THAT USE HEAT SHOCK PROTEINS |
| US5935576A (en) | 1995-09-13 | 1999-08-10 | Fordham University | Compositions and methods for the treatment and prevention of neoplastic diseases using heat shock proteins complexed with exogenous antigens |
| US6017540A (en) | 1997-02-07 | 2000-01-25 | Fordham University | Prevention and treatment of primary and metastatic neoplastic diseases and infectious diseases with heat shock/stress protein-peptide complexes |
| GB9818133D0 (en) | 1998-08-19 | 1998-10-14 | Quadrant Holdings Cambridge | Vaccine |
| GB9919734D0 (en) | 1999-08-19 | 1999-10-20 | Colaco Camilo | Vaccines from infectious agents |
| GB9919733D0 (en) | 1999-08-19 | 1999-10-20 | Colaco Camilo | Vaccines against intracellular pathogens |
| AUPQ233799A0 (en) | 1999-08-19 | 1999-09-09 | Minister For Agriculture, Minister For Land And Water Conservation For And On Behalf Of The State Of New South Wales | Recombinant sub-unit vaccine |
| GB0021757D0 (en) | 2000-09-04 | 2000-10-18 | Colaco Camilo | Vaccine against microbial pathogens |
| WO2002028407A1 (en) | 2000-09-29 | 2002-04-11 | University Of Connecticut Health Center | Methods of recovering heat shock proteins and complexes thereof |
| US20020172682A1 (en) * | 2000-10-20 | 2002-11-21 | University Of Connecticut Health Center | Using heat shock proteins to increase immune response |
| US7132109B1 (en) * | 2000-10-20 | 2006-11-07 | University Of Connecticut Health Center | Using heat shock proteins to increase immune response |
| US6875849B2 (en) * | 2001-05-01 | 2005-04-05 | Arizona Board Of Regents Of Behalf Of The University Of Arizona | Methods of recovering chaperone proteins and complexes thereof |
| CN1665533A (zh) | 2002-05-02 | 2005-09-07 | 康涅狄格大学健康中心 | 热激蛋白用于增强抗体疗法功效的用途 |
| EP2145896A3 (en) * | 2003-11-12 | 2010-03-31 | Alfa Biogene International B.V. | Recovery of a heat shock protein |
| GB0816242D0 (en) | 2008-09-05 | 2008-10-15 | Immunobiology Ltd | Method of purifying protien complexes |
-
2009
- 2009-06-19 GB GBGB0910591.7A patent/GB0910591D0/en not_active Ceased
-
2010
- 2010-06-21 AU AU2010261505A patent/AU2010261505B2/en not_active Ceased
- 2010-06-21 EP EP10732406.3A patent/EP2442825B1/en active Active
- 2010-06-21 JP JP2012515567A patent/JP5778666B2/ja not_active Expired - Fee Related
- 2010-06-21 US US13/378,808 patent/US10040820B2/en active Active
- 2010-06-21 CN CN201080031703.0A patent/CN102458460B/zh active Active
- 2010-06-21 IN IN250DEN2012 patent/IN2012DN00250A/en unknown
- 2010-06-21 WO PCT/GB2010/051023 patent/WO2010146401A1/en not_active Ceased
-
2012
- 2012-01-18 ZA ZA2012/00420A patent/ZA201200420B/en unknown
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