JP2012529894A - 細胞を単離する方法 - Google Patents

細胞を単離する方法 Download PDF

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Publication number
JP2012529894A
JP2012529894A JP2012515373A JP2012515373A JP2012529894A JP 2012529894 A JP2012529894 A JP 2012529894A JP 2012515373 A JP2012515373 A JP 2012515373A JP 2012515373 A JP2012515373 A JP 2012515373A JP 2012529894 A JP2012529894 A JP 2012529894A
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JP
Japan
Prior art keywords
cells
sample
mgcl
extraction solution
atoms
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Pending
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JP2012515373A
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English (en)
Japanese (ja)
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JP2012529894A5 (pl
Inventor
ロスマニス，ペーター
メスター，パトリック，ユリアン
ワグナー，マルティン
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Merck Patent GmbH
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Merck Patent GmbH
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Publication date
Application filed by Merck Patent GmbH filed Critical Merck Patent GmbH
Publication of JP2012529894A publication Critical patent/JP2012529894A/ja
Publication of JP2012529894A5 publication Critical patent/JP2012529894A5/ja
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/24—Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C12N5/0025—Culture media for plant cell or plant tissue culture

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Botany (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
JP2012515373A 2009-06-18 2010-05-31 細胞を単離する方法 Pending JP2012529894A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP09007959 2009-06-18
EP09007959.1 2009-06-18
PCT/EP2010/003295 WO2010145754A1 (en) 2009-06-18 2010-05-31 Method for isolating cells

Publications (2)

Publication Number Publication Date
JP2012529894A true JP2012529894A (ja) 2012-11-29
JP2012529894A5 JP2012529894A5 (pl) 2015-05-28

Family

ID=42313799

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2012515373A Pending JP2012529894A (ja) 2009-06-18 2010-05-31 細胞を単離する方法

Country Status (7)

Country Link
US (2) US20120122110A1 (pl)
EP (2) EP2853587B1 (pl)
JP (1) JP2012529894A (pl)
CN (1) CN102459567B (pl)
ES (2) ES2656098T3 (pl)
PL (1) PL2443226T3 (pl)
WO (1) WO2010145754A1 (pl)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103492550A (zh) 2011-04-27 2014-01-01 默克专利股份公司 用于裂解细胞的方法
GB201617713D0 (en) 2016-10-19 2016-11-30 Q-Linea Ab Method for recovering microbial cells
CN108949631A (zh) * 2018-07-30 2018-12-07 骆奇 一种核孔膜在肠道内容物分离中的应用

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH04228097A (ja) * 1990-07-02 1992-08-18 Toyo Ink Mfg Co Ltd ミルク試料からの細胞分離および濃縮方法とそのキット
US5346994A (en) * 1992-01-28 1994-09-13 Piotr Chomczynski Shelf-stable product and process for isolating RNA, DNA and proteins
CN1226421C (zh) * 2003-10-21 2005-11-09 南京农业大学 松材线虫检测试剂盒及其检测方法
AT501194A1 (de) * 2004-12-30 2006-07-15 Thomas Dr Schlederer Verfahren zur isolierung von zellen und viren
EP1882738A4 (en) * 2005-05-20 2009-02-25 Arkray Inc METHOD FOR OBTAINING MICROORGANISMS AND NUCLEIC ACIDS USING FINE PARTICLES AND FOR THE METHOD OF USING THE KIT
EP2049677B1 (en) * 2006-08-10 2011-07-20 Merck Patent GmbH Method for isolating cells

Also Published As

Publication number Publication date
ES2656098T3 (es) 2018-02-23
WO2010145754A1 (en) 2010-12-23
US20140045189A1 (en) 2014-02-13
EP2853587A1 (en) 2015-04-01
EP2443226A1 (en) 2012-04-25
PL2443226T3 (pl) 2015-04-30
US20120122110A1 (en) 2012-05-17
CN102459567B (zh) 2016-03-09
EP2853587B1 (en) 2017-10-11
ES2529215T3 (es) 2015-02-18
CN102459567A (zh) 2012-05-16
EP2443226B1 (en) 2014-11-19

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