JP2012529894A - 細胞を単離する方法 - Google Patents
細胞を単離する方法 Download PDFInfo
- Publication number
- JP2012529894A JP2012529894A JP2012515373A JP2012515373A JP2012529894A JP 2012529894 A JP2012529894 A JP 2012529894A JP 2012515373 A JP2012515373 A JP 2012515373A JP 2012515373 A JP2012515373 A JP 2012515373A JP 2012529894 A JP2012529894 A JP 2012529894A
- Authority
- JP
- Japan
- Prior art keywords
- cells
- sample
- mgcl
- extraction solution
- atoms
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/24—Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0018—Culture media for cell or tissue culture
- C12N5/0025—Culture media for plant cell or plant tissue culture
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Pathology (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Botany (AREA)
- Cell Biology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP09007959 | 2009-06-18 | ||
EP09007959.1 | 2009-06-18 | ||
PCT/EP2010/003295 WO2010145754A1 (en) | 2009-06-18 | 2010-05-31 | Method for isolating cells |
Publications (2)
Publication Number | Publication Date |
---|---|
JP2012529894A true JP2012529894A (ja) | 2012-11-29 |
JP2012529894A5 JP2012529894A5 (pl) | 2015-05-28 |
Family
ID=42313799
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2012515373A Pending JP2012529894A (ja) | 2009-06-18 | 2010-05-31 | 細胞を単離する方法 |
Country Status (7)
Country | Link |
---|---|
US (2) | US20120122110A1 (pl) |
EP (2) | EP2853587B1 (pl) |
JP (1) | JP2012529894A (pl) |
CN (1) | CN102459567B (pl) |
ES (2) | ES2656098T3 (pl) |
PL (1) | PL2443226T3 (pl) |
WO (1) | WO2010145754A1 (pl) |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP6147730B2 (ja) | 2011-04-27 | 2017-06-14 | メルク パテント ゲゼルシャフト ミット ベシュレンクテル ハフツングMerck Patent Gesellschaft mit beschraenkter Haftung | 細胞を溶解する方法 |
GB201617713D0 (en) | 2016-10-19 | 2016-11-30 | Q-Linea Ab | Method for recovering microbial cells |
CN108949631A (zh) * | 2018-07-30 | 2018-12-07 | 骆奇 | 一种核孔膜在肠道内容物分离中的应用 |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH04228097A (ja) * | 1990-07-02 | 1992-08-18 | Toyo Ink Mfg Co Ltd | ミルク試料からの細胞分離および濃縮方法とそのキット |
US5346994A (en) * | 1992-01-28 | 1994-09-13 | Piotr Chomczynski | Shelf-stable product and process for isolating RNA, DNA and proteins |
CN1226421C (zh) * | 2003-10-21 | 2005-11-09 | 南京农业大学 | 松材线虫检测试剂盒及其检测方法 |
AT501194A1 (de) * | 2004-12-30 | 2006-07-15 | Thomas Dr Schlederer | Verfahren zur isolierung von zellen und viren |
JPWO2006123781A1 (ja) * | 2005-05-20 | 2008-12-25 | アークレイ株式会社 | 微粒子を用いた微生物及び核酸の回収方法ならびにそれらに用いるキット |
US9328326B2 (en) | 2006-08-10 | 2016-05-03 | Merck Patent Gmbh | Method for isolating cells |
-
2010
- 2010-05-31 PL PL10723931T patent/PL2443226T3/pl unknown
- 2010-05-31 ES ES14003562.7T patent/ES2656098T3/es active Active
- 2010-05-31 JP JP2012515373A patent/JP2012529894A/ja active Pending
- 2010-05-31 ES ES10723931.1T patent/ES2529215T3/es active Active
- 2010-05-31 EP EP14003562.7A patent/EP2853587B1/en active Active
- 2010-05-31 CN CN201080027681.0A patent/CN102459567B/zh active Active
- 2010-05-31 EP EP10723931.1A patent/EP2443226B1/en active Active
- 2010-05-31 US US13/378,690 patent/US20120122110A1/en not_active Abandoned
- 2010-05-31 WO PCT/EP2010/003295 patent/WO2010145754A1/en active Application Filing
-
2013
- 2013-10-21 US US14/058,772 patent/US20140045189A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
ES2656098T3 (es) | 2018-02-23 |
EP2443226A1 (en) | 2012-04-25 |
US20120122110A1 (en) | 2012-05-17 |
PL2443226T3 (pl) | 2015-04-30 |
WO2010145754A1 (en) | 2010-12-23 |
EP2853587A1 (en) | 2015-04-01 |
CN102459567B (zh) | 2016-03-09 |
CN102459567A (zh) | 2012-05-16 |
EP2443226B1 (en) | 2014-11-19 |
EP2853587B1 (en) | 2017-10-11 |
US20140045189A1 (en) | 2014-02-13 |
ES2529215T3 (es) | 2015-02-18 |
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