JP2012527283A - 合成移植片 - Google Patents
合成移植片 Download PDFInfo
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- JP2012527283A JP2012527283A JP2012511342A JP2012511342A JP2012527283A JP 2012527283 A JP2012527283 A JP 2012527283A JP 2012511342 A JP2012511342 A JP 2012511342A JP 2012511342 A JP2012511342 A JP 2012511342A JP 2012527283 A JP2012527283 A JP 2012527283A
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- gel
- epithelium
- artificial
- collagen gel
- artificial eye
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- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2227/00—Animals characterised by species
- A01K2227/10—Mammal
- A01K2227/107—Rabbit
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2267/00—Animals characterised by purpose
- A01K2267/03—Animal model, e.g. for test or diseases
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2430/00—Materials or treatment for tissue regeneration
- A61L2430/16—Materials or treatment for tissue regeneration for reconstruction of eye parts, e.g. intraocular lens, cornea
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2502/00—Coculture with; Conditioned medium produced by
- C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
- C12N2502/1323—Adult fibroblasts
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/50—Proteins
- C12N2533/52—Fibronectin; Laminin
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/50—Proteins
- C12N2533/54—Collagen; Gelatin
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/90—Substrates of biological origin, e.g. extracellular matrix, decellularised tissue
- C12N2533/92—Amnion; Decellularised dermis or mucosa
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Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
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| GBGB0908927.7A GB0908927D0 (en) | 2009-05-22 | 2009-05-22 | Synthetic graft |
| GB0908927.7 | 2009-05-22 | ||
| PCT/GB2010/001024 WO2010133853A1 (en) | 2009-05-22 | 2010-05-21 | Synthetic graft |
Publications (2)
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| JP2012527283A true JP2012527283A (ja) | 2012-11-08 |
| JP2012527283A5 JP2012527283A5 (enExample) | 2013-07-04 |
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| JP2012511342A Pending JP2012527283A (ja) | 2009-05-22 | 2010-05-21 | 合成移植片 |
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| US (1) | US20120148543A1 (enExample) |
| EP (1) | EP2432871A1 (enExample) |
| JP (1) | JP2012527283A (enExample) |
| CN (1) | CN102449142A (enExample) |
| CA (1) | CA2762362A1 (enExample) |
| GB (2) | GB0908927D0 (enExample) |
| WO (1) | WO2010133853A1 (enExample) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2014200182A (ja) * | 2013-04-03 | 2014-10-27 | 国立大学法人 東京大学 | 培地及び細胞の培養方法 |
| JP2019504708A (ja) * | 2016-02-11 | 2019-02-21 | ライフセル コーポレーションLifeCell Corporation | 酵素分解に対するコラーゲン含有組織製品の安定化方法 |
| JP2023553217A (ja) * | 2020-12-11 | 2023-12-20 | オステオジェニックス バイオメディカル インコーポレイテッド | 多層コラーゲンベース膜 |
| JP2024510296A (ja) * | 2021-03-18 | 2024-03-06 | ザ・ユニバーシティ・オブ・シドニー | バイオエンジニアリングされた内皮構築物 |
Families Citing this family (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11542328B2 (en) | 2008-11-14 | 2023-01-03 | The Brigham And Women's Hospital, Inc. | Therapeutic and diagnostic methods relating to cancer stem cells |
| US8468344B2 (en) | 2009-05-26 | 2013-06-18 | Raytheon Company | Enabling multi-level security in a single-level security computing system |
| WO2011154687A1 (en) * | 2010-06-11 | 2011-12-15 | Ucl Business Plc | Biomimetic corneal tissue |
| US9622911B2 (en) | 2010-09-30 | 2017-04-18 | Cxl Ophthalmics, Llc | Ophthalmic treatment device, system, and method of use |
| WO2012073238A1 (en) * | 2010-12-02 | 2012-06-07 | Technion Research & Development Foundation Ltd. | Methods of generating corneal cells and cell populations comprising same |
| EP4420725A3 (en) | 2012-03-29 | 2025-04-16 | Epion Therapeutics, Inc. | Ocular treatment solutions, delivery devices and delivery augmentation methods |
| US9566301B2 (en) | 2012-03-29 | 2017-02-14 | Cxl Ophthalmics, Llc | Compositions and methods for treating or preventing diseases associated with oxidative stress |
| ES2860573T3 (es) * | 2013-02-19 | 2021-10-05 | Childrens Medical Center | Células madre ABCB5(+) para el tratamiento de enfermedades oculares |
| BR112015028193B8 (pt) | 2013-05-10 | 2023-04-18 | Childrens Medical Center | Suporte para cicatrização de feridas |
| CN105979912B (zh) * | 2013-12-03 | 2021-08-20 | 康奈尔大学 | 修复环的方法和胶原凝胶组合物 |
| GB201402787D0 (en) * | 2014-02-17 | 2014-04-02 | Univ Newcastle | Expansion method |
| WO2015189266A1 (en) * | 2014-06-10 | 2015-12-17 | Ludwig Boltzmann Gesellschaft | Biocompatibility assay |
| US20170233698A1 (en) * | 2014-06-27 | 2017-08-17 | The Regents Of The University Of California | Cultured mammalian limbal stem cells, methods for generating the same, and uses thereof |
| CN104368046B (zh) * | 2014-11-10 | 2016-01-13 | 四川大学 | 一种纤维增强型载药水凝胶人工角膜裙边支架及其制备方法 |
| SE539167C2 (en) | 2015-12-22 | 2017-05-02 | Rafat Mehrdad | A composite collagen hydrogel material, an implantable ophthalmic device comprising such material and methods of producing the composite collagen hydrogel material and the implantable ophthalmic device |
| CN106854637A (zh) * | 2016-12-14 | 2017-06-16 | 浙江大学 | 一种猪甲状腺的3d细胞培养方法 |
| CN114917058A (zh) * | 2021-12-16 | 2022-08-19 | 首都医科大学附属北京同仁医院 | 一种便于使用的组织工程角膜上皮植片及其制备、应用方法 |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2008504921A (ja) * | 2004-07-05 | 2008-02-21 | ユーシーエル ビジネス ピーエルシー | 組織等価物の細胞非依存的製造 |
| WO2009004351A2 (en) * | 2007-07-05 | 2009-01-08 | Ucl Business Plc | Methods for producing biomaterials with variable stiffness |
| JP2009517112A (ja) * | 2005-11-25 | 2009-04-30 | ユーシーエル ビジネス ピーエルシー | 調整可能特性を有する生体人工材料 |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4600533A (en) | 1984-12-24 | 1986-07-15 | Collagen Corporation | Collagen membranes for medical use |
| US20020039788A1 (en) * | 2000-02-29 | 2002-04-04 | Isseroff Roslyn R. | Corneal epithelial graft composites |
| US20060014284A1 (en) | 2002-03-21 | 2006-01-19 | Thomas Graeve | Biomatrix and method for producting the same |
| WO2005087285A1 (ja) * | 2004-03-11 | 2005-09-22 | Arblast Co., Ltd. | 角膜上皮シート及びその作製方法、並びに同シートを用いる移植方法 |
| ITRM20040273A1 (it) * | 2004-06-03 | 2004-09-03 | Farmigea Spa | Metodo per l'isolamento e l'espansione ex vivo di cellule staminali corneali umane e loro relativi usi. |
| US7173631B2 (en) * | 2004-09-23 | 2007-02-06 | Qualcomm Incorporated | Flexible antialiasing in embedded devices |
| US20080065207A1 (en) * | 2006-03-13 | 2008-03-13 | University Of South Florida | Self-Assembling, Collagen-Based Material for Corneal Replacement |
| US20080260794A1 (en) * | 2007-02-12 | 2008-10-23 | Lauritzen Nels J | Collagen products and methods for producing collagen products |
-
2009
- 2009-05-22 GB GBGB0908927.7A patent/GB0908927D0/en active Pending
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2010
- 2010-05-21 EP EP10721543A patent/EP2432871A1/en not_active Withdrawn
- 2010-05-21 CA CA2762362A patent/CA2762362A1/en not_active Abandoned
- 2010-05-21 US US13/321,603 patent/US20120148543A1/en not_active Abandoned
- 2010-05-21 CN CN2010800221926A patent/CN102449142A/zh active Pending
- 2010-05-21 GB GB1008576A patent/GB2470644A/en not_active Withdrawn
- 2010-05-21 WO PCT/GB2010/001024 patent/WO2010133853A1/en not_active Ceased
- 2010-05-21 JP JP2012511342A patent/JP2012527283A/ja active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2008504921A (ja) * | 2004-07-05 | 2008-02-21 | ユーシーエル ビジネス ピーエルシー | 組織等価物の細胞非依存的製造 |
| JP2009517112A (ja) * | 2005-11-25 | 2009-04-30 | ユーシーエル ビジネス ピーエルシー | 調整可能特性を有する生体人工材料 |
| WO2009004351A2 (en) * | 2007-07-05 | 2009-01-08 | Ucl Business Plc | Methods for producing biomaterials with variable stiffness |
Non-Patent Citations (6)
| Title |
|---|
| ADVANCED FUNCTIONAL MATERIALS, vol. 15, JPN6014026843, 2005, pages 1762 - 1770, ISSN: 0002842943 * |
| AMBROSE WINNETTE MCINTOSH: "COLLAGEN VITRIGEL MEMBRANES FOR THE IN VITRO RECONSTRUCTION OF SEPARATE CORNEAL EPITHELIAL,以下備考", JOURNAL OF BIOMEDICAL MATERIALS RESEARCH, vol. V90B N2, JPN5012013387, 3 March 2009 (2009-03-03), pages 818 - 831, ISSN: 0002842939 * |
| BUILLES N: "DEVELOPMENT OF A HEMICORNEA FROM HUMAN PRIMARY CELL CULTURES FOR PHARMACOTOXICOLOGY TESTING", CELL BIOLOGY AND TOXICOLOGY, vol. V23 N4, JPN5012013386, 13 February 2007 (2007-02-13), pages 279 - 292, ISSN: 0002842938 * |
| IBUSUKI SHINICHI: "PHOTOCHEMICALLY CROSS-LINKED COLLAGEN GELS AS THRESS-DIMENSIONAL SCAFFOLDS FOR TISSUE ENGINEERING", TISSUE ENGINEERING, vol. V13 N8, JPN5012013388, August 2007 (2007-08-01), pages 1995 - 2001, ISSN: 0002842942 * |
| INVESTIGATIVE OPHTHALMOLOGY AND VISUAL SCIENCE, vol. 35, JPN6014026846, 1994, pages 479 - 485, ISSN: 0002842940 * |
| JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE, vol. 2, JPN6014026842, 2008, pages 263 - 271, ISSN: 0002842941 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2014200182A (ja) * | 2013-04-03 | 2014-10-27 | 国立大学法人 東京大学 | 培地及び細胞の培養方法 |
| JP2019504708A (ja) * | 2016-02-11 | 2019-02-21 | ライフセル コーポレーションLifeCell Corporation | 酵素分解に対するコラーゲン含有組織製品の安定化方法 |
| US11179505B2 (en) | 2016-02-11 | 2021-11-23 | Lifecell Corporation | Methods for stabilizing collagen-containing tissue products against enzymatic degradation |
| JP2023553217A (ja) * | 2020-12-11 | 2023-12-20 | オステオジェニックス バイオメディカル インコーポレイテッド | 多層コラーゲンベース膜 |
| JP2024510296A (ja) * | 2021-03-18 | 2024-03-06 | ザ・ユニバーシティ・オブ・シドニー | バイオエンジニアリングされた内皮構築物 |
Also Published As
| Publication number | Publication date |
|---|---|
| GB201008576D0 (en) | 2010-07-07 |
| GB0908927D0 (en) | 2009-07-01 |
| CA2762362A1 (en) | 2010-11-25 |
| EP2432871A1 (en) | 2012-03-28 |
| CN102449142A (zh) | 2012-05-09 |
| GB2470644A (en) | 2010-12-01 |
| US20120148543A1 (en) | 2012-06-14 |
| WO2010133853A1 (en) | 2010-11-25 |
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