JP2012515548A - 単一細胞の細胞傷害性を査定するための組成物および方法 - Google Patents

単一細胞の細胞傷害性を査定するための組成物および方法 Download PDF

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JP2012515548A
JP2012515548A JP2011547913A JP2011547913A JP2012515548A JP 2012515548 A JP2012515548 A JP 2012515548A JP 2011547913 A JP2011547913 A JP 2011547913A JP 2011547913 A JP2011547913 A JP 2011547913A JP 2012515548 A JP2012515548 A JP 2012515548A
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microwell
hiv
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ジョン クリストファー ラヴ
ナビーン バラダラヤン
ボリス ジュルグ
ブルース ウォーカー
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Massachusetts Institute of Technology
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966Animal cells
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    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5047Cells of the immune system
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/52Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/563Immunoassay; Biospecific binding assay; Materials therefor involving antibody fragments
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    • C40COMBINATORIAL TECHNOLOGY
    • C40BCOMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B30/00Methods of screening libraries
    • C40B30/06Methods of screening libraries by measuring effects on living organisms, tissues or cells
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/005Assays involving biological materials from specific organisms or of a specific nature from viruses
    • G01N2333/08RNA viruses
    • G01N2333/15Retroviridae, e.g. bovine leukaemia virus, feline leukaemia virus, feline leukaemia virus, human T-cell leukaemia-lymphoma virus
    • G01N2333/155Lentiviridae, e.g. visna-maedi virus, equine infectious virus, FIV, SIV
    • G01N2333/16HIV-1, HIV-2

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  • General Engineering & Computer Science (AREA)
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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
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JP2011547913A 2009-01-21 2009-07-13 単一細胞の細胞傷害性を査定するための組成物および方法 Withdrawn JP2012515548A (ja)

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US14610609P 2009-01-21 2009-01-21
US61/146,106 2009-01-21
PCT/US2009/050411 WO2010085275A1 (en) 2009-01-21 2009-07-13 Compositions and methods for assessing cytotoxicity of single cells

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Cited By (5)

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WO2017169267A1 (ja) * 2016-03-30 2017-10-05 ソニー株式会社 細胞観察装置、免疫細胞の活性度の評価方法及び免疫細胞の品質管理方法
JPWO2020171020A1 (ja) * 2019-02-18 2021-03-11 株式会社エヌビィー健康研究所 細胞の選抜方法、核酸の製造方法、組換え細胞の製造方法、目的物質の製造方法、医薬組成物の製造方法、及び試薬
JP2022087206A (ja) * 2016-11-11 2022-06-09 アイソプレキシス コーポレイション 単一細胞のゲノム、トランスクリプトームおよびプロテオームの同時解析のための組成物および方法
US11518977B2 (en) 2014-01-31 2022-12-06 AgBiome, Inc. Modified biological control agents and their uses
US11760971B2 (en) 2014-01-31 2023-09-19 AgBiome, Inc. Modified biological control agents and their uses

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EP2370814B1 (en) 2008-12-04 2015-04-29 Massachusetts Institute of Technology Method for diagnosing allergic reactions
EP2389446B1 (en) 2009-01-21 2017-07-05 Massachusetts Institute of Technology Compositions and methods for assessing cytotoxicity of single cells
WO2012072823A1 (en) * 2010-12-03 2012-06-07 Mindseeds Laboratories Srl Rapid screening of monoclonal antibodies
CN103392124B (zh) 2010-12-03 2016-04-20 塞普莱有限公司 细胞功能的微分析
US9944983B2 (en) 2011-12-13 2018-04-17 Single Cell Technology, Inc. Method of screening a plurality of single secreting cells for functional activity
US20150191772A1 (en) 2012-06-29 2015-07-09 Danmarks Tekniske Universitet Method of charging a test carrier and a test carrier
CN104884605B (zh) * 2012-08-24 2018-05-18 耶鲁大学 用于高通量多重检测的系统、装置和方法
WO2015031190A1 (en) * 2013-08-26 2015-03-05 President And Fellows Of Harvard College Determination of immune cells and other cells
EP3227684B1 (en) 2014-12-03 2019-10-02 Isoplexis Corporation Analysis and screening of cell secretion profiles
US10488321B2 (en) 2015-03-19 2019-11-26 The Board Of Trustees Of The Leland Stanford Junior University Devices and methods for high-throughput single cell and biomolecule analysis and retrieval in a microfluidic chip
JP6855382B2 (ja) 2015-03-26 2021-04-14 ユニバーシティー オブ ヒューストン システム 細胞の統合された機能性および分子のプロファイリング
EP3402902B1 (en) 2016-01-15 2021-10-27 Massachusetts Institute Of Technology Semi-permeable arrays for analyzing biological systems and methods of using same
WO2017165791A1 (en) 2016-03-25 2017-09-28 President And Fellows Of Harvard College Microfluidic determination of immune and other cells
US12138625B2 (en) 2016-06-14 2024-11-12 Cellply S.R.L Screening kit and method
EP3472351B1 (de) 2016-06-15 2020-10-28 Ludwig-Maximilians-Universität München Einzelmolekülnachweis bzw. -quantifizierung durch dna-nanotechnologie
CN106086171A (zh) * 2016-06-15 2016-11-09 广州中大南沙科技创新产业园有限公司 一种抑制肿瘤细胞增殖的药物筛选方法
CN109964126B (zh) 2016-09-12 2022-12-27 伊索普莱克西斯公司 用于细胞治疗法和其他免疫治疗法的多重分析的系统和方法
AU2017345402A1 (en) * 2016-10-19 2019-05-23 General Automation Lab Technologies Inc. High resolution systems, kits, apparatus, and methods for screening microorganisms and other high throughput microbiology applications
US11085912B2 (en) * 2016-12-14 2021-08-10 University Of Cincinnati Methods of diagnosing Clostridium difficile infection or recurrence in a subject
SE543211C2 (en) * 2017-06-29 2020-10-27 Mabtech Production Ab Method and system for analyzing Fluorospot assays
JP2021505157A (ja) 2017-12-07 2021-02-18 マサチューセッツ インスティテュート オブ テクノロジー 単一細胞分析
CN108728356B (zh) * 2018-05-23 2022-02-22 北京科技大学 用于不同三维细胞团配对的装置及共培养方法
CN113614532A (zh) * 2019-01-16 2021-11-05 烟台澳斯邦生物工程有限公司 沉积用于显微镜检查的生物样品的自动液体处理系统和方法
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Cited By (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11518977B2 (en) 2014-01-31 2022-12-06 AgBiome, Inc. Modified biological control agents and their uses
US12398364B2 (en) 2014-01-31 2025-08-26 Certis U.S.A. L.L.C. Modified biological control agents and their uses
US11760971B2 (en) 2014-01-31 2023-09-19 AgBiome, Inc. Modified biological control agents and their uses
CN108779427A (zh) * 2016-03-30 2018-11-09 索尼公司 细胞观察装置、用于评估免疫细胞活性水平的方法以及用于控制免疫细胞品质的方法
JPWO2017169267A1 (ja) * 2016-03-30 2019-03-14 ソニー株式会社 細胞観察装置、免疫細胞の活性度の評価方法及び免疫細胞の品質管理方法
JP7021633B2 (ja) 2016-03-30 2022-02-17 ソニーグループ株式会社 細胞観察装置及び免疫細胞の品質管理方法
WO2017169267A1 (ja) * 2016-03-30 2017-10-05 ソニー株式会社 細胞観察装置、免疫細胞の活性度の評価方法及び免疫細胞の品質管理方法
JP2022087206A (ja) * 2016-11-11 2022-06-09 アイソプレキシス コーポレイション 単一細胞のゲノム、トランスクリプトームおよびプロテオームの同時解析のための組成物および方法
JP7568672B2 (ja) 2016-11-11 2024-10-16 アイソプレキシス コーポレイション 単一細胞のゲノム、トランスクリプトームおよびプロテオームの同時解析のための組成物および方法
US11357787B2 (en) 2019-02-18 2022-06-14 Nb Health Laboratory Co., Ltd. Method for selecting cells, method for producing nucleic acid, method for producing recombinant cells, method for producing target substance, method for producing pharmaceutical composition, and reagent
JP2021118722A (ja) * 2019-02-18 2021-08-12 株式会社エヌビィー健康研究所 細胞の選抜方法、核酸の製造方法、組換え細胞の製造方法、目的物質の製造方法、医薬組成物の製造方法、及び試薬
JP7456627B2 (ja) 2019-02-18 2024-03-27 株式会社エヌビィー健康研究所 細胞の選抜方法、核酸の製造方法、組換え細胞の製造方法、目的物質の製造方法、医薬組成物の製造方法、及び試薬
US12208113B2 (en) 2019-02-18 2025-01-28 Nb Health Laboratory Co., Ltd. Method for selecting cells, method for producing nucleic acid, method for producing recombinant cells, method for producing target substance, method for producing pharmaceutical composition, and reagent
JPWO2020171020A1 (ja) * 2019-02-18 2021-03-11 株式会社エヌビィー健康研究所 細胞の選抜方法、核酸の製造方法、組換え細胞の製造方法、目的物質の製造方法、医薬組成物の製造方法、及び試薬

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EP2389446A1 (en) 2011-11-30
AU2009338127B2 (en) 2015-11-26
KR20110134386A (ko) 2011-12-14
CA2689681A1 (en) 2010-07-21
RU2011134898A (ru) 2013-02-27
SG172889A1 (en) 2011-08-29
CN102388145A (zh) 2012-03-21
US20120149592A1 (en) 2012-06-14
EP2389446B1 (en) 2017-07-05
CA2689681C (en) 2017-08-29
US9244071B2 (en) 2016-01-26
CN104745669A (zh) 2015-07-01
RU2532228C2 (ru) 2014-10-27
WO2010085275A1 (en) 2010-07-29
AU2009338127A1 (en) 2011-07-28
JP2015144617A (ja) 2015-08-13
EP2389446A4 (en) 2012-10-03
IL213938A0 (en) 2011-07-31
SG10201401643PA (en) 2014-08-28
JP6017620B2 (ja) 2016-11-02

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