JP2012512633A - エンドヌクレアーゼ活性を有するポリペプチド断片、及びそれらの使用 - Google Patents

エンドヌクレアーゼ活性を有するポリペプチド断片、及びそれらの使用 Download PDF

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Publication number
JP2012512633A
JP2012512633A JP2011541228A JP2011541228A JP2012512633A JP 2012512633 A JP2012512633 A JP 2012512633A JP 2011541228 A JP2011541228 A JP 2011541228A JP 2011541228 A JP2011541228 A JP 2011541228A JP 2012512633 A JP2012512633 A JP 2012512633A
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Prior art keywords
subunit
compound
polypeptide fragment
seq
amino acid
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JP2011541228A
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Japanese (ja)
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JP2012512633A5 (enExample
Inventor
ブーヴィエ,デニス
クレパン,シバート
リイグロック,ロブ
ディアス,アレキサンダー
キューザック,ステファン
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Europaisches Laboratorium fuer Molekularbiologie EMBL
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Europaisches Laboratorium fuer Molekularbiologie EMBL
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Publication of JP2012512633A publication Critical patent/JP2012512633A/ja
Publication of JP2012512633A5 publication Critical patent/JP2012512633A5/ja
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    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C07K14/08RNA viruses
    • C07K14/11Orthomyxoviridae, e.g. influenza virus
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00Medicinal preparations containing organic active ingredients
    • A61K31/185Acids; Anhydrides, halides or salts thereof, e.g. sulfur acids, imidic, hydrazonic or hydroximic acids
    • A61K31/19Carboxylic acids, e.g. valproic acid
    • A61K31/192Carboxylic acids, e.g. valproic acid having aromatic groups, e.g. sulindac, 2-aryl-propionic acids, ethacrynic acid 
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12Antivirals
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12Antivirals
    • A61P31/14Antivirals for RNA viruses
    • A61P31/16Antivirals for RNA viruses for influenza or rhinoviruses
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/12Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241Nucleotidyltransferases (2.7.7)
    • C12N9/127RNA-directed RNA polymerase (2.7.7.48), i.e. RNA replicase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/44Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving esterase
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/573Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • GPHYSICS
    • G16INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16BBIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B15/00ICT specially adapted for analysing two-dimensional or three-dimensional molecular structures, e.g. structural or functional relations or structure alignment
    • GPHYSICS
    • G16INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16BBIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B15/00ICT specially adapted for analysing two-dimensional or three-dimensional molecular structures, e.g. structural or functional relations or structure alignment
    • G16B15/20Protein or domain folding
    • GPHYSICS
    • G16INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16BBIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B5/00ICT specially adapted for modelling or simulations in systems biology, e.g. gene-regulatory networks, protein interaction networks or metabolic networks
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2299/00Coordinates from 3D structures of peptides, e.g. proteins or enzymes
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90Enzymes; Proenzymes
    • G01N2333/914Hydrolases (3)
    • G01N2333/916Hydrolases (3) acting on ester bonds (3.1), e.g. phosphatases (3.1.3), phospholipases C or phospholipases D (3.1.4)
    • G01N2333/922Ribonucleases (RNAses); Deoxyribonucleases (DNAses)
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00Screening for compounds of potential therapeutic value
    • G01N2500/02Screening involving studying the effect of compounds C on the interaction between interacting molecules A and B (e.g. A = enzyme and B = substrate for A, or A = receptor and B = ligand for the receptor)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Virology (AREA)
  • Immunology (AREA)
  • Evolutionary Biology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Medical Informatics (AREA)
  • Theoretical Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Communicable Diseases (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Cell Biology (AREA)
JP2011541228A 2008-12-19 2009-12-18 エンドヌクレアーゼ活性を有するポリペプチド断片、及びそれらの使用 Pending JP2012512633A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US20325908P 2008-12-19 2008-12-19
US61/203,259 2008-12-19
PCT/EP2009/009161 WO2010069600A2 (en) 2008-12-19 2009-12-18 Polypeptide fragments comprising endonuclease activity and their use

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2015127283A Division JP2016013124A (ja) 2008-12-19 2015-06-25 エンドヌクレアーゼ活性を有するポリペプチド断片、及びそれらの使用

Publications (2)

Publication Number Publication Date
JP2012512633A true JP2012512633A (ja) 2012-06-07
JP2012512633A5 JP2012512633A5 (enExample) 2013-01-24

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Application Number Title Priority Date Filing Date
JP2011541228A Pending JP2012512633A (ja) 2008-12-19 2009-12-18 エンドヌクレアーゼ活性を有するポリペプチド断片、及びそれらの使用
JP2015127283A Pending JP2016013124A (ja) 2008-12-19 2015-06-25 エンドヌクレアーゼ活性を有するポリペプチド断片、及びそれらの使用
JP2017099300A Pending JP2017205107A (ja) 2008-12-19 2017-05-18 エンドヌクレアーゼ活性を有するポリペプチド断片、及びそれらの使用

Family Applications After (2)

Application Number Title Priority Date Filing Date
JP2015127283A Pending JP2016013124A (ja) 2008-12-19 2015-06-25 エンドヌクレアーゼ活性を有するポリペプチド断片、及びそれらの使用
JP2017099300A Pending JP2017205107A (ja) 2008-12-19 2017-05-18 エンドヌクレアーゼ活性を有するポリペプチド断片、及びそれらの使用

Country Status (13)

Country Link
US (3) US20120108493A1 (enExample)
EP (2) EP2379715B1 (enExample)
JP (3) JP2012512633A (enExample)
KR (5) KR20110127114A (enExample)
CN (1) CN102449144B (enExample)
AU (1) AU2009328502B2 (enExample)
BR (1) BRPI0923134A2 (enExample)
CA (1) CA2745532A1 (enExample)
ES (1) ES2604227T3 (enExample)
IL (1) IL213360A0 (enExample)
MX (1) MX2011006539A (enExample)
SG (1) SG172239A1 (enExample)
WO (1) WO2010069600A2 (enExample)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015050170A1 (ja) * 2013-10-02 2015-04-09 学校法人 久留米大学 インフルエンザウイルス阻害薬
JP2018150260A (ja) * 2017-03-10 2018-09-27 地方独立行政法人神奈川県立産業技術総合研究所 インフルエンザウイルスの増殖を阻害するモノクローナル抗体
JP2019528508A (ja) * 2016-07-07 2019-10-10 ヨーロピアン モレキュラー バイオロジー ラボラトリーEuropean Molecular Biology Laboratory 細胞性rnaポリメラーゼiiのc末端ドメイン(ctd)に結合するウイルスポリペプチド断片およびそれらの使用

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101792745B (zh) * 2009-02-04 2014-09-17 中国科学院生物物理研究所 流感病毒聚合酶亚基pa氨基端多肽的表达纯化及pa氨基端多肽的晶体结构
US8859290B2 (en) * 2011-07-22 2014-10-14 Rutgers, The State University Of New Jersey Inhibitors of influenza endonuclease activity and tools for their discovery
CN114496063B (zh) * 2022-01-13 2023-05-23 北京博康健基因科技有限公司 基于天然氨基酸序列的信号肽设计与二级结构从头算建模方法和装置

Citations (3)

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JP2006504415A (ja) * 2002-10-16 2006-02-09 イシス イノベイション リミテッド アスパラギニルヒドロキシラーゼ及びその修飾物質
JP2006525032A (ja) * 2003-05-02 2006-11-09 イーラン ファーマスーティカルズ、インコーポレイテッド Baceのグリコシル化変異体
JP2008526263A (ja) * 2005-01-18 2008-07-24 ヌベロ, インコーポレイテッド 幹細胞因子様タンパク質をコードするポリヌクレオチド

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US6482414B1 (en) * 1998-08-13 2002-11-19 The University Of Pittsburgh-Of The Commonwealth System Of Higher Education Cold-adapted equine influenza viruses
IL153005A0 (en) * 2000-06-23 2003-06-24 American Cyanamid Co Nucleotide sequence of influenza a/udorn/72 (h3n2) genome
WO2003092607A2 (en) * 2002-05-01 2003-11-13 Vertex Pharmaceuticals Incorporated Crystal structure of aurora-2 protein and binding pockets thereof

Patent Citations (3)

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JP2006504415A (ja) * 2002-10-16 2006-02-09 イシス イノベイション リミテッド アスパラギニルヒドロキシラーゼ及びその修飾物質
JP2006525032A (ja) * 2003-05-02 2006-11-09 イーラン ファーマスーティカルズ、インコーポレイテッド Baceのグリコシル化変異体
JP2008526263A (ja) * 2005-01-18 2008-07-24 ヌベロ, インコーポレイテッド 幹細胞因子様タンパク質をコードするポリヌクレオチド

Non-Patent Citations (3)

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JPN5012005524; Journal of Virology Vol. 70, No. 3, 1996, pp.1905-1911 *
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JPN5012005526; Journal of Virology Vol. 76, No. 18, 2002, pp.8989-9001 *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015050170A1 (ja) * 2013-10-02 2015-04-09 学校法人 久留米大学 インフルエンザウイルス阻害薬
JPWO2015050170A1 (ja) * 2013-10-02 2017-03-09 学校法人 久留米大学 インフルエンザウイルス阻害薬
JP2019528508A (ja) * 2016-07-07 2019-10-10 ヨーロピアン モレキュラー バイオロジー ラボラトリーEuropean Molecular Biology Laboratory 細胞性rnaポリメラーゼiiのc末端ドメイン(ctd)に結合するウイルスポリペプチド断片およびそれらの使用
JP2018150260A (ja) * 2017-03-10 2018-09-27 地方独立行政法人神奈川県立産業技術総合研究所 インフルエンザウイルスの増殖を阻害するモノクローナル抗体

Also Published As

Publication number Publication date
SG172239A1 (en) 2011-07-28
EP2623590A2 (en) 2013-08-07
IL213360A0 (en) 2011-07-31
CA2745532A1 (en) 2010-06-24
AU2009328502B2 (en) 2015-02-05
WO2010069600A8 (en) 2011-07-21
EP2623590A3 (en) 2013-11-13
KR20160017131A (ko) 2016-02-15
EP2379715B1 (en) 2016-10-26
EP2379715A2 (en) 2011-10-26
CN102449144A (zh) 2012-05-09
US20120108493A1 (en) 2012-05-03
KR20160017132A (ko) 2016-02-15
BRPI0923134A2 (pt) 2017-03-28
KR20160113335A (ko) 2016-09-28
US20150105315A1 (en) 2015-04-16
US9890368B2 (en) 2018-02-13
KR20170061193A (ko) 2017-06-02
US20180010109A1 (en) 2018-01-11
JP2016013124A (ja) 2016-01-28
WO2010069600A3 (en) 2010-08-05
KR20110127114A (ko) 2011-11-24
CN102449144B (zh) 2015-08-26
AU2009328502A1 (en) 2011-07-14
MX2011006539A (es) 2011-10-21
ES2604227T3 (es) 2017-03-03
WO2010069600A2 (en) 2010-06-24
JP2017205107A (ja) 2017-11-24

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