JP2012505657A - 遺伝子発現の阻害のためのショートヘアピンｒｎａ - Google Patents

遺伝子発現の阻害のためのショートヘアピンｒｎａ Download PDF

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Publication number
JP2012505657A
JP2012505657A JP2011532222A JP2011532222A JP2012505657A JP 2012505657 A JP2012505657 A JP 2012505657A JP 2011532222 A JP2011532222 A JP 2011532222A JP 2011532222 A JP2011532222 A JP 2011532222A JP 2012505657 A JP2012505657 A JP 2012505657A
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sequence
nucleotides
rna
rna molecule
region
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JP2012505657A5 (enExample
Inventor
ガー，チン
ジョンストン，ブライアン，エイチ．
カザコフ，セルゲイ，エイ．
イルベス，ハイニ
ダラス，アン
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Somagenics Inc
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Somagenics Inc
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Publication of JP2012505657A publication Critical patent/JP2012505657A/ja
Publication of JP2012505657A5 publication Critical patent/JP2012505657A5/ja
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/16—Drugs for disorders of the alimentary tract or the digestive system for liver or gallbladder disorders, e.g. hepatoprotective agents, cholagogues, litholytics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A61P31/14—Antivirals for RNA viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1131—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering nucleic acids [NA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/50—Physical structure
    • C12N2310/53—Physical structure partially self-complementary or closed
    • C12N2310/531—Stem-loop; Hairpin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/50—Physical structure
    • C12N2310/53—Physical structure partially self-complementary or closed
    • C12N2310/533—Physical structure partially self-complementary or closed having a mismatch or nick in at least one of the strands

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Virology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Veterinary Medicine (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Public Health (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Communicable Diseases (AREA)
  • Oncology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2011532222A 2008-10-15 2009-10-14 遺伝子発現の阻害のためのショートヘアピンｒｎａ Pending JP2012505657A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US10560608P 2008-10-15 2008-10-15
US61/105,606 2008-10-15
PCT/US2009/060712 WO2010045384A2 (en) 2008-10-15 2009-10-14 Short hairpin rnas for inhibition of gene expression

Related Child Applications (1)

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JP2015122459A Division JP2015211680A (ja) 2008-10-15 2015-06-17 遺伝子発現の阻害のためのショートヘアピンｒｎａ

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JP2012505657A true JP2012505657A (ja) 2012-03-08
JP2012505657A5 JP2012505657A5 (enExample) 2012-12-06

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JP2015122459A Pending JP2015211680A (ja) 2008-10-15 2015-06-17 遺伝子発現の阻害のためのショートヘアピンｒｎａ

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Country Link
US (2) US8283460B2 (enExample)
EP (1) EP2352744B1 (enExample)
JP (2) JP2012505657A (enExample)
CN (1) CN102245772A (enExample)
WO (1) WO2010045384A2 (enExample)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019022196A1 (ja) * 2017-07-26 2019-01-31 日産化学株式会社 一本鎖オリゴヌクレオチド

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006031901A2 (en) * 2004-09-10 2006-03-23 Somagenics, Inc. SMALL INTERFERING RNAs THAT EFFICIENTLY INHIBIT VIRAL GENE EXPRESSION AND METHODS OF USE THEREOF
WO2010045384A2 (en) 2008-10-15 2010-04-22 Somagenics Inc. Short hairpin rnas for inhibition of gene expression
US8871730B2 (en) 2009-07-13 2014-10-28 Somagenics Inc. Chemical modification of short small hairpin RNAs for inhibition of gene expression
US8796238B2 (en) * 2009-09-17 2014-08-05 Sigma-Aldrich Co. Llc Short RNA mimetics
PT2561078T (pt) * 2010-04-23 2018-12-03 Cold Spring Harbor Laboratory Sharn com uma conceção estrutural inovadora
JP2016514091A (ja) 2013-02-08 2016-05-19 ミスフォールディング ダイアグノスティクス， インコーポレイテッド トランスサイレチン抗体およびその使用
US10947543B2 (en) * 2013-03-13 2021-03-16 Yale University Interferon production using short RNA duplexes
US10450569B2 (en) * 2015-08-13 2019-10-22 Somagenics, Inc. Methods and compositions of short small hairpin RNAS and microRNAS for wound healing
CN112204141A (zh) * 2018-02-22 2021-01-08 美国陶氏益农公司 短/小发夹rna分子
EP3810151B1 (en) 2018-06-20 2025-08-06 Yale University Rig-i agonists and treatments using same
US11786545B2 (en) 2020-12-09 2023-10-17 Yale University Compositions and methods for treating SARS-CoV-2 infection
US11649457B2 (en) 2020-12-09 2023-05-16 Yale University Methods for treating SARS-CoV-2 infection
WO2023122805A1 (en) 2021-12-20 2023-06-29 Vestaron Corporation Sorbitol driven selection pressure method

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2004500018A (ja) * 1999-03-09 2004-01-08 バイオオーリアンス　ファーマ（エス．エー．） ２次構造によって安定化されたアンチセンス配列を含有するオリゴヌクレオチド及びそれを含有する薬学組成物
US20040053876A1 (en) * 2002-03-26 2004-03-18 The Regents Of The University Of Michigan siRNAs and uses therof
WO2005079533A2 (en) * 2004-02-17 2005-09-01 University Of Massachusetts Methods and compositions for mediating gene silencing
US20060134787A1 (en) * 2004-12-22 2006-06-22 University Of Massachusetts Methods and compositions for enhancing the efficacy and specificity of single and double blunt-ended siRNA
WO2006078414A2 (en) * 2004-12-23 2006-07-27 Ambion, Inc. METHODS AND COMPOSITIONS CONCERNING siRNA'S AS MEDIATORS OF RNA INTERFERENCE
WO2007032794A2 (en) * 2005-09-12 2007-03-22 Somagenics Inc. Inhibition of viral gene expression using small interfering rna
JP2007529994A (ja) * 2003-07-07 2007-11-01 ワン セル システムズ インコーポレイテッド ヘアピン標識したプローブと使用方法

Family Cites Families (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6174868B1 (en) 1992-09-10 2001-01-16 Isis Pharmaceuticals, Inc. Compositions and methods for treatment of hepatitis C virus-associated diseases
JPH10503364A (ja) 1994-05-10 1998-03-31 ザ ジェネラル ホスピタル コーポレーション Ｃ型肝炎ウイルスのアンチセンス阻害
DE19956568A1 (de) * 1999-01-30 2000-08-17 Roland Kreutzer Verfahren und Medikament zur Hemmung der Expression eines vorgegebenen Gens
US8202979B2 (en) * 2002-02-20 2012-06-19 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid
CN100523215C (zh) 2000-12-01 2009-08-05 马普科技促进协会 介导rna干涉的小rna分子
EP1386004A4 (en) 2001-04-05 2005-02-16 Ribozyme Pharm Inc MODULATION OF GENE EXPRESSION ASSOCIATED WITH INFLAMMATORY PROLIFERATION AND GROWTH OF NEURITIES BY METHODS INVOLVING NUCLEIC ACID
US20040209831A1 (en) 2002-02-20 2004-10-21 Mcswiggen James RNA interference mediated inhibition of hepatitis C virus (HCV) gene expression using short interfering nucleic acid (siNA)
US10590418B2 (en) 2001-07-23 2020-03-17 The Board Of Trustees Of The Leland Stanford Junior University Methods and compositions for RNAi mediated inhibition of gene expression in mammals
US20040138163A1 (en) * 2002-05-29 2004-07-15 Mcswiggen James RNA interference mediated inhibition of vascular edothelial growth factor and vascular edothelial growth factor receptor gene expression using short interfering nucleic acid (siNA)
US7294504B1 (en) 2001-12-27 2007-11-13 Allele Biotechnology & Pharmaceuticals, Inc. Methods and compositions for DNA mediated gene silencing
JP2005517427A (ja) 2002-02-20 2005-06-16 サーナ・セラピューティクス・インコーポレイテッド 短干渉核酸（ｓｉＮＡ）を用いるＣ型肝炎ウイルス（ＨＣＶ）遺伝子発現のＲＮＡ干渉媒介性阻害
AU2003256857A1 (en) 2002-08-08 2004-02-25 Dharmacon, Inc. Short interfering rnas having a hairpin structure containing a non-nucleotide loop
JP4948163B2 (ja) * 2003-05-23 2012-06-06 サーナ・セラピューティクス・インコーポレイテッド 化学修飾した低分子干渉核酸（ｓｉＮＡ）を使用する遺伝子発現のＲＮＡ干渉仲介抑制
US7750144B2 (en) 2003-06-02 2010-07-06 University Of Massachusetts Methods and compositions for enhancing the efficacy and specificity of RNA silencing
US20050130919A1 (en) * 2003-07-18 2005-06-16 University Of Massachusetts Regulatable promoters for synthesis of small hairpin RNA
WO2005028650A2 (en) * 2003-09-16 2005-03-31 Sirna Therapeutics, Inc. RNA INTERFERENCE MEDIATED INHIBITION OF HEPATITIS C VIRUS (HCV) EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA)
US20090004739A1 (en) 2003-09-22 2009-01-01 Taku Demura Efficient Method of Preparing Dna Inverted Repeat
US20050164210A1 (en) 2004-01-23 2005-07-28 Vivek Mittal Regulated polymerase III expression systems and related methods
US20090035784A1 (en) * 2004-07-30 2009-02-05 Mount Sinai School Of Medicine Of New York University Npc1l1 and npc1l1 inhibitors and methods of use thereof
WO2006031901A2 (en) 2004-09-10 2006-03-23 Somagenics, Inc. SMALL INTERFERING RNAs THAT EFFICIENTLY INHIBIT VIRAL GENE EXPRESSION AND METHODS OF USE THEREOF
JP2008526213A (ja) * 2004-12-30 2008-07-24 トッド エム． ハウザー， 自己保護オリゴヌクレオチドを使用する、遺伝子発現を調節するための組成物および方法
US20060223777A1 (en) * 2005-03-29 2006-10-05 Dharmacon, Inc. Highly functional short hairpin RNA
US20070048759A1 (en) 2005-06-10 2007-03-01 Dan Luo Detection of target molecules with labeled nucleic acid detection molecules
US20070259827A1 (en) * 2006-01-25 2007-11-08 University Of Massachusetts Compositions and methods for enhancing discriminatory RNA interference
ES2556128T3 (es) * 2006-03-23 2016-01-13 Roche Innovation Center Copenhagen A/S ARN interfiriente pequeño internamente segmentado
EP3121281B1 (en) * 2007-08-27 2021-03-31 1Globe Health Institute LLC Compositions of asymmetric interfering rna and uses thereof
KR100949791B1 (ko) * 2007-12-18 2010-03-30 이동기 오프-타겟 효과를 최소화하고 ＲＮＡｉ 기구를 포화시키지않는 신규한 ｓｉＲＮＡ 구조 및 그 용도
WO2010045384A2 (en) 2008-10-15 2010-04-22 Somagenics Inc. Short hairpin rnas for inhibition of gene expression

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2004500018A (ja) * 1999-03-09 2004-01-08 バイオオーリアンス　ファーマ（エス．エー．） ２次構造によって安定化されたアンチセンス配列を含有するオリゴヌクレオチド及びそれを含有する薬学組成物
US20040053876A1 (en) * 2002-03-26 2004-03-18 The Regents Of The University Of Michigan siRNAs and uses therof
JP2007529994A (ja) * 2003-07-07 2007-11-01 ワン セル システムズ インコーポレイテッド ヘアピン標識したプローブと使用方法
WO2005079533A2 (en) * 2004-02-17 2005-09-01 University Of Massachusetts Methods and compositions for mediating gene silencing
US20060134787A1 (en) * 2004-12-22 2006-06-22 University Of Massachusetts Methods and compositions for enhancing the efficacy and specificity of single and double blunt-ended siRNA
WO2006078414A2 (en) * 2004-12-23 2006-07-27 Ambion, Inc. METHODS AND COMPOSITIONS CONCERNING siRNA'S AS MEDIATORS OF RNA INTERFERENCE
WO2007032794A2 (en) * 2005-09-12 2007-03-22 Somagenics Inc. Inhibition of viral gene expression using small interfering rna

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
JPN6014015342; ＲＮＡ ｖｏｌ．８, 2002, ｐｐ．８４２-８５０ *
JPN6014015343; Ｎｕｃｌｅｉｃ　Ａｃｉｄｓ　Ｒｅｓｅａｒｃｈ ｖｏｌ．３６　ｎｏ．１８, 2008, ｐｐ．５８１２-５８２１ *
JPN6014015345; ＧＥＮＥ ｖｏｌ．３５６, 2005, ｐｐ．３２-３８ *
JPN6014015346; ＡＮＴＩＳＥＮＳＥ　ＡＮＤ　ＮＵＣＬＥＩＣ　ＡＣＩＤ　ＤＲＵＧ　ＤＥＶＥＬＯＰＭＥＮＴ ｖｏｌ．１３, 2003, ｐｐ．８３-１０５ *
JPN6014015348; ＯＬＩＧＯＮＵＣＬＥＯＴＩＤＥＳ ｖｏｌ．１７, 2007, ｐｐ．２２３-２３６ *

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019022196A1 (ja) * 2017-07-26 2019-01-31 日産化学株式会社 一本鎖オリゴヌクレオチド
JPWO2019022196A1 (ja) * 2017-07-26 2020-05-28 日産化学株式会社 一本鎖オリゴヌクレオチド
US11555188B2 (en) 2017-07-26 2023-01-17 Nissan Chemical Corporation Single-stranded oligonucleotide
JP7384033B2 (ja) 2017-07-26 2023-11-21 日産化学株式会社 一本鎖オリゴヌクレオチド
JP2024020326A (ja) * 2017-07-26 2024-02-14 日産化学株式会社 一本鎖オリゴヌクレオチド
JP7690979B2 (ja) 2017-07-26 2025-06-11 日産化学株式会社 一本鎖オリゴヌクレオチド

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Publication number Publication date
EP2352744A2 (en) 2011-08-10
US20100112686A1 (en) 2010-05-06
EP2352744B1 (en) 2016-09-21
WO2010045384A2 (en) 2010-04-22
EP2352744A4 (en) 2013-04-10
US8779115B2 (en) 2014-07-15
CN102245772A (zh) 2011-11-16
WO2010045384A3 (en) 2010-06-10
US8283460B2 (en) 2012-10-09
JP2015211680A (ja) 2015-11-26
US20120329857A1 (en) 2012-12-27

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