JP2012219027A - Hair tonic - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide the new application of fucoxanthin and/or fucoxantinol in which the functions of a diabetes improving function, a vascularization inhibiting action, a DHA synthesis promoting action or the like are known.SOLUTION: The hair tonic comprises fucoxanthin and/or fucoxanenthinol as an active constituent. The increase in a hair growth rate by a mouse has been confirmed by using the active constituent. Further, a hair growing effect, a depilation effect and an aging preventing effect in a human have been confirmed by using the active constituent.

Description

The present invention relates to a hair restorer.

Fucoxanthin is known to have functions such as diabetes-improving action (Patent Document 1), angiogenesis-inhibiting action (Patent Document 2), and DHA synthesis promoting action (Patent Document 3).

JP 2007-314451 A JP 2008-1623 A JP 2007-77067 A

An object of the present invention is to find a new use of fucoxanthin or a similar compound thereof, and to provide a hair growth agent having an excellent hair growth effect.

The present invention provides a hair restorer containing fucoxanthin and / or fucoxanthinol as an active ingredient.

ADVANTAGE OF THE INVENTION According to this invention, the new use of fucoxanthin and / or fucoxanthinol can be found, and the hair restoring agent excellent in the hair restoring effect can be made.

Fucoxanthin and fucoxanthinol used in the present invention are known compounds, and their preparation methods are described in, for example, Patent Document 2. That is, the fucoxanthin used in the present invention can be derived from a natural product, for example, brown algae belonging to the order of the family Kombuaceae, Chigasiaceae, etc .; It can be extracted from microalgae such as diatoms.

The extraction method is performed, for example, by bringing a polar solvent such as a polar organic solvent or a mixture of water and a polar organic solvent into contact with a raw material. Examples of the polar organic solvent include lower alcohols having 1 to 5 carbon atoms such as methanol, ethanol, butanol and isopropanol; propylene glycol; acetone; ethyl acetate; hexane; dichloromethane; chloroform alone or a combination of two or more. Can do. In addition, it is preferable to dry and grind the raw material containing fucoxanthin prior to extraction. The extraction method may be performed by a conventional method. For example, fucoxanthin can be extracted by adding 2 to 100 times the above-described extraction solvent to the raw material, and subjecting the raw material to immersion treatment and stirring treatment.

It is preferable to remove the organic solvent from the extracted fucoxanthin by vacuum distillation or the like. It can also be applied to various known purification methods. If necessary, it can be further subjected to a drying treatment such as reduced pressure drying, freeze drying, spray drying and the like. In the present invention, various commercially available fucoxanthins can also be used.

Fucoxanthinol is a hydrolyzed fucoxanthin and is known. The hydrolysis reaction can be achieved by, for example, fucoxanthin using a lipolytic enzyme such as lipase or cholesterol esterase.

When the hair restorer of the present invention is orally administered, the dose of fucoxanthin and / or fucoxanthinol varies depending on the age, body weight, indication symptoms, etc. of the patient. It is preferable to administer 1 mg to 1 g, preferably about 3 mg to 300 mg.

When applying the hair restorer of the present invention to the scalp, it is usually used in the form of an aqueous solution or dispersion. In this case, the amount of the active ingredient of the present invention is preferably 0.00001 to 10% by mass, more preferably 0.0001 to 5% by mass, and particularly preferably 0.01 to 4% by mass. Such a hair-growth agent may be applied to the scalp in an amount of 0.1 ml to 10 ml, preferably 0.5 ml to 2 ml once a day for adults 1 to 3 times a day.

The hair restorer of the present invention can be used in various forms, and examples other than oral administration include forms such as hair art, hair cream, hair lotion, rinse, shampoo, hair foam, hair gel, aerosol and the like. .

Moreover, the hair restorer of this invention can also add various additives as needed. For example, humectants, thickeners, fragrances, preservatives, ultraviolet absorbers, anti-inflammatory agents, vitamins and the like can be mentioned.

EXAMPLES Hereinafter, although an Example demonstrates this invention further, this invention is not limited to the following example.

Preparation Example Fucoxanthin and fucoxanthinol were prepared according to the method described in Patent Document 2 above. The method is shown below. 1L of acetone: methanol (7: 3, v / v) was added in several batches to commercially available dried seaweed powder (dry weight about 100g) that was pulverized with a pulverizer. A fraction was obtained. After removing the solvent with an evaporator and dissolving in 100 mL of methanol, 100 mL of hexane and 10 mL of water were added, and liquid-liquid distribution was performed using a separatory funnel.

The upper layer of the obtained solution was removed, and the lower layer was washed twice with 100 mL of hexane. Thereafter, 31 mL of water was added to the lower layer to make the ratio of methanol 70%, and then 100 mL of hexane was added for distribution. Furthermore, after concentrating the lower layer with an evaporator until the volume became 1/3, 150 mL of diethyl ether and 100 mL of water were added and distributed.

The upper layer (ether layer) was collected, the solvent was removed by an evaporator, and the obtained extract was subjected to thin layer chromatography (developing solvent = hexane: acetone (6: 4, v / v); PLC plate (15 PLC plates 20 × 20 cm, Silica gel 60 F254, 1 mm, Merck Ltd., Japan)). The separated carotenoid fraction was scraped off from the plate and extracted again to obtain a carotenoid extract.

The carotenoid extract obtained above is subjected to HPLC, a fraction of the peak portion of fucoxanthin appearing at a measurement wavelength of 444 nm and a retention time of 8.7 minutes is fractionated, and the fraction thus fractioned with dichloromethane and Extraction with water was purified. The HPLC conditions are as shown below. When fucoxanthin was dissolved in acetone and calculated using the specific absorbance as a concentration, about 11 mg of fucoxanthin was obtained. This operation was repeated several times and freeze-dried to obtain powder 1 used in the following examples. (HPLC preparative conditions) -flow rate 1.0 mL / min-column temperature 40 ° C.-mobile phase (A) acetonitrile: methanol: water = 75: 15: 10 (v / v / v, containing 0.1% ammonium acetate) ) And (B) 2-component gradient using ethyl acetate: methanol = 30: 70 (v / v, containing 0.1% ammonium acetate)

Further, fucoxanthin obtained as described above was enzymatically degraded using cholesterol esterase to obtain fucoxanthinol.

Example 1 The hair growth effect of fucoxanthin and fucoxanthinol was verified according to the experimental procedure described in Japanese Patent No. 4041451. (1) Tests with mice C3H mice (8 weeks old, male, average body weight 35g) dorsal skin (2cm x 4cm) were cut with an electric clipper and shaver, and from the next day, each sample was applied to the test subject's skin twice a day in the morning and evening. Then, 0.2 mL per animal was applied continuously for 2 weeks. A group of 10 animals was used for one sample. On the 22nd day from the start of application, the skin of the test area of each sample was photographed with a video camera, and the areas of the hair cutting part and the hair growth part were measured with an image analyzer. The hair growth effect was determined by calculating the hair growth rate (%) shown below, and comparing the average hair growth rate of each group of Examples or Comparative Examples. Hair growth rate (%) = (Area of hair growth part) / (Area of hair cutting part) × 100

(2) Human test At 7 cm above the ears of 7 subjects with male pattern baldness, the head hair was shaved into a circular shape with a diameter of 5 mm at two left and right sides, and either one of the samples was placed on the entire left hair. The sample was applied twice a day in the morning and evening, about 3 mL for 3 months, and the right side was compared without any application. The hair growth effect, hair removal effect, and anti-dandruff effect were determined by comparing the test site with no application on the right side for each item 3 months after the start of the test. ”,“ Less hair loss ”and“ Less dandruff ”.

A hair restorer having the formulation shown in Table 1 was prepared according to a conventional method, and the above tests were conducted for evaluation. The results are also shown in Table 1.

From the results in Table 1, it was found that fucoxanthin and fucoxanthinol have a hair growth effect.

Claims (1)

A hair restorer comprising fucoxanthin and / or fucoxanthinol as an active ingredient.