JP4113518B2 - TGF-β production promoter for suppressing hair growth of body hair - Google Patents
TGF-β production promoter for suppressing hair growth of body hair Download PDFInfo
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- JP4113518B2 JP4113518B2 JP2004150245A JP2004150245A JP4113518B2 JP 4113518 B2 JP4113518 B2 JP 4113518B2 JP 2004150245 A JP2004150245 A JP 2004150245A JP 2004150245 A JP2004150245 A JP 2004150245A JP 4113518 B2 JP4113518 B2 JP 4113518B2
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- Cosmetics (AREA)
- Medicines Containing Plant Substances (AREA)
Description
本発明は、体毛の毛成長抑制のためのＴＧＦ−β産生促進剤に関する。 The present invention relates to a TGF-β production promoter for suppressing hair growth of body hair.
近年、手、足、腋等における体毛は美的外観上、無い方が好ましいとする傾向が高まり、このため各種の体毛除去方法が開発され、利用されている。具体的にはシェーバー、抜毛器等を用いる機械的除去方法、脱毛剤を用いて体毛を毛根から抜毛する方法、除毛剤を用いてその化学的作用により体毛を除去する方法などが挙げられる。 In recent years, there is an increasing tendency that hair in hands, feet, heels and the like is preferable in terms of aesthetic appearance, and various hair removal methods have been developed and used. Specific examples include a mechanical removal method using a shaver, hair remover, etc., a method of removing hair from the hair root using a hair removal agent, and a method of removing body hair by its chemical action using a hair removal agent.
しかしながら、これらの体毛除去方法は、皮膚に対して物理的または化学的刺激を伴うものであり、また、体毛除去方法によっては多少の差はあるものの体毛除去効果の持続性には限度がある。このため、一定期間経過後には再び体毛除去処理を行わなければならず、体毛除去処理の軽減化が望まれている。 However, these hair removal methods involve physical or chemical irritation to the skin, and the durability of the hair removal effect is limited although there are some differences depending on the hair removal method. For this reason, the hair removal process must be performed again after a certain period of time, and reduction of the hair removal process is desired.
このような観点から、毛成長を抑制する抑制剤が開発されており、そのような抑制剤に関する特許出願として、たとえば下記特許文献１のような出願がなされている。この特許文献１に記載された毛成長の抑制剤は、パントテン酸を配合したものである。
しかしながら、この特許文献１には、パントテン酸が毛成長を抑制する作用について明確に記載されておらず、毛の量を秤量することによる試験結果が開示されているのみで、その効果の有用性も定かではない。 However, this Patent Document 1 does not clearly describe the action of pantothenic acid to suppress hair growth, and only discloses the test results obtained by weighing the amount of hair. Not sure.
一方、毛成長抑制剤及びその抑制剤を配合した化粧料に関する特許出願として下記特許文献２のような出願もなされている。この特許文献２に記載された毛成長抑制剤は、フラボノール配糖体を有効成分とするものである。
この特許文献２には、不死化ヒト外毛根鞘細胞の増殖抑制試験により、その細胞増殖抑制率を算出して増殖抑制の効果が示されている。しかし、この特許文献２においても、毛成長を抑制する作用については定かでなく、効果についても十分とは必ずしも認められない。 This Patent Document 2 shows the effect of inhibiting growth by calculating the cell growth inhibition rate by a growth inhibition test of immortalized human outer root sheath cells. However, also in this patent document 2, it is not certain about the effect | action which suppresses hair growth, and it is not necessarily recognized as sufficient about an effect.
本発明は、このような問題を解決するためになされたもので、体毛除去処理の軽減化を図ることのできる毛成長抑制剤、並びにその毛成長抑制剤を配合した皮膚外用剤及び化粧料を提供することを課題とする。 The present invention has been made in order to solve such problems, and includes a hair growth inhibitor capable of reducing body hair removal treatment, and a skin external preparation and a cosmetic containing the hair growth inhibitor. The issue is to provide.
体毛の毛根には毛乳頭と称される組織があり、毛乳頭が種々のサイトカインを産生することにより、毛成長をコントロールすることが近年報告されている。毛成長を抑制するサイトカインとしてＴＧＦ−β（トランスフォーミング成長因子−β）がある。従って、毛乳頭が産生するＴＧＦ−βの産生を促進させることにより、体毛の成長を抑制することができると考えられる。 There is a tissue called a hair papilla in the hair root of body hair, and it has recently been reported that the hair papilla produces various cytokines to control hair growth. There is TGF-β (transforming growth factor-β) as a cytokine that suppresses hair growth. Therefore, it is considered that the growth of body hair can be suppressed by promoting the production of TGF-β produced by the hair papilla.
本発明者等はこのような点に着目し、各種植物抽出物の毛成長抑制作用について鋭意検討した結果、イラクサ抽出物が毛乳頭によるＴＧＦ−βの産生を促進し、優れた毛成長抑制効果を有することを見出し、本発明を完成するに至った。 As a result of intensive studies on the hair growth inhibitory action of various plant extracts, the present inventors have promoted the production of TGF-β by the hair papilla and have an excellent hair growth inhibitory effect. As a result, the present invention has been completed.
すなわち請求項１記載の発明は、体毛の毛成長抑制のためのＴＧＦ−β産生促進剤に、イラクサ抽出物を有効成分として含有させたことを特徴とする。ここで「イラクサ抽出物を有効成分として含有する」とは、ＴＧＦ−β産生促進剤がイラクサ抽出物のみからなっていてもよく、またイラクサ抽出物以外の成分を含んでいてもよいことを意味する。 That is, the invention described in claim 1 is characterized in that a nettle extract is contained as an active ingredient in a TGF-β production promoter for suppressing hair growth of body hair . Here, “contains nettle extract as an active ingredient” means that the TGF-β production promoter may consist of nettle extract alone or may contain components other than nettle extract. To do.
本発明によって、優れた毛成長抑制効果を有し、人体に対して安全性の高い毛成長抑制剤、及びその毛成長抑制剤を配合した皮膚外用剤、化粧料を提供することが可能となった。 According to the present invention, it is possible to provide a hair growth inhibitor that has an excellent hair growth inhibitory effect and is highly safe for the human body, and an external preparation for skin and a cosmetic that contain the hair growth inhibitor. It was.
本発明の体毛の毛成長抑制のためのＴＧＦ−β産生促進剤の有効成分として用いられるイラクサ（Urtica thubergiana Siebold et)は、イラクサ科に属し、本州、四国、九州に分布する多年草の植物である。本発明で用いられる抽出物とは、イラクサの粉砕物を、常温または加温下に溶剤により抽出するか、または、ソックスレー抽出器等の抽出器具を用いて抽出することにより得られる各種溶媒抽出液、その希釈液、その濃縮液、あるいはその乾燥末を意味するものである。抽出材料となるイラクサの部位は特に限定されるものではないが、葉を材料とするのが好ましい。 The nettle (Urtica thubergiana Siebold et) used as an active ingredient of the TGF-β production promoter for inhibiting hair growth of the present invention belongs to the nettle family and is a perennial plant distributed in Honshu, Shikoku and Kyushu. . The extract used in the present invention is an extract of various solvents obtained by extracting a ground nettle with a solvent at room temperature or under heating, or by extracting it using an extraction device such as a Soxhlet extractor. , Its diluted solution, its concentrated solution, or its dry powder. The part of the nettle used as the extraction material is not particularly limited, but leaves are preferred.
抽出に用いる溶媒としては、通常の植物の抽出に用いられる溶媒であれば任意に用いることができる。例えば、水、メタノール、エタノール、プロピレングリコール、１，３−ブチレングリコール、グリセリン等のアルコール類、含水アルコール類、クロロホルム、ジクロルエタン、四塩化炭素、アセトン、酢酸エチル、ヘキサン等の有機溶媒類等であり、それらは単独あるいは組み合わせて用いることができる。 As a solvent used for extraction, any solvent can be used as long as it is a solvent used for normal plant extraction. For example, water, alcohols such as methanol, ethanol, propylene glycol, 1,3-butylene glycol, glycerin, hydrous alcohols, organic solvents such as chloroform, dichloroethane, carbon tetrachloride, acetone, ethyl acetate, hexane, etc. They can be used alone or in combination.
上記のような本発明のＴＧＦ−β産生促進剤を配合した皮膚外用剤、化粧料は、ゲル状、ローション等の液状、クリーム状、エアゾール等の剤型にすることができるが、これらの剤型に限定されるものではない。皮膚外用剤としては、たとえば医薬部外品や医薬品等に適用するものが用いられる。 Skin Hadagaiyo agent formulated with TGF-beta production promoter of the present invention as described above, cosmetics, gel, liquid lotions, creams, can be on the dosage form of aerosols or the like, these It is not limited to the dosage form. As a skin external preparation, what is applied to a quasi drug, a pharmaceutical, etc. is used, for example.
本発明のＴＧＦ−β産生促進剤（イラクサ抽出物）の皮膚外用剤、化粧料中の配合量は、通常乾燥固形分として０．０００１〜５０重量％とすることが好ましい。０．０００１重量％未満では本発明の効果が充分に得られない可能性があり、一方、５０重量％を越えても、その増量に見合った効果の向上は認められないからである。この観点からは、０．００１〜１０重量％が特に好ましい。 Skin Hadagaiyo agent TGF-beta production promoter of the present invention (nettle extract), the amount of the cosmetic composition is preferably set to 0.0001 wt% as a normal dry solids. If the amount is less than 0.0001% by weight, the effect of the present invention may not be sufficiently obtained. On the other hand, if the amount exceeds 50% by weight, an improvement in the effect commensurate with the increase is not recognized. From this viewpoint, 0.001 to 10% by weight is particularly preferable.
本発明の皮膚外用剤や化粧料には、本発明の効果を損なわない範囲において、一般に化粧料に用いられ、或いは医薬部外品や医薬品等の皮膚外用剤に用いられる各種任意成分を必要に応じて適宜配合することができる。このような任意成分としては、例えば、精製水、エタノール、油性成分、保湿剤、増粘剤、防腐剤、乳化剤、薬効成分、粉体、紫外線吸収剤、色素、香料、乳化安定剤等を挙げることができる。 The skin external preparation and cosmetics of the present invention require various optional ingredients that are generally used in cosmetics or used in skin external preparations such as quasi-drugs and pharmaceuticals, as long as the effects of the present invention are not impaired. It can mix | blend suitably according to it. Examples of such optional components include purified water, ethanol, oily components, humectants, thickeners, preservatives, emulsifiers, medicinal components, powders, ultraviolet absorbers, dyes, fragrances, and emulsion stabilizers. be able to.
以下、本発明の実施例について説明する。
（実施例１）
本実施例の体毛の毛成長抑制のためのＴＧＦ−β産生促進剤は、イラクサ（Urtica thubergiana Siebold et)の葉の抽出成分からなるものである。その１つの製造例について説明すると、イラクサ（Urtica thubergiana Siebold et)の葉（乾燥物）２００ｇを２L の５０％エタノール溶液に浸漬し、６０〜８０℃で２日間抽出し、抽出液から溶媒を除去し、１５．８ｇの抽出物を得た。抽出物の量は、乾燥固形分としての値で示している。
Examples of the present invention will be described below.
(Example 1)
TGF-beta production promoter for hair hair growth inhibition of this embodiment is made of extracted components of leaves of nettle (Urtica thubergiana Siebold et). To explain one example of production, 200 g of nettle (Urtica thubergiana Siebold et) leaves (dried material) was immersed in 2 L of 50% ethanol solution, extracted at 60-80 ° C. for 2 days, and the solvent was removed from the extract. 15.8 g of extract was obtained. The amount of the extract is shown as a value as a dry solid content.
（試験例１）ヒト毛乳頭細胞のＴＧＦ−β産生能に対する作用試験
ヒト毛乳頭細胞を１２wellプレートに２×１０4 ／wellで播種し、１０％牛胎児血清を添加したダルベッコ変法ＭＥＭ培地（ＤＭＥＭ培地）で５日間培養した。培養５日目にＤＭＥＭ培地で細胞を洗浄した後、各濃度（０．００００１重量％、０．０００１重量％、０．００１重量％）のイラクサ抽出物を含むＤＭＥＭ培地を加え、さらに培養を続けた。
(Test Example 1) Test of human hair papilla cells on the ability to produce TGF-β Human hair papilla cells were seeded at 2 × 10 4 / well in a 12-well plate and Dulbecco's modified MEM medium (DMEM supplemented with 10% fetal calf serum). Medium) for 5 days. After washing the cells with DMEM medium on the 5th day of culture, DMEM medium containing nettle extract of each concentration (0.00001 wt%, 0.0001 wt%, 0.001 wt%) was added, and the culture was further continued. It was.
３日間培養した後、培養上澄を回収し、培養上澄に含まれるＴＧＦ−βの量をヒトＴＧＦ−β Immunoassay キット（ＧＴ社製）を用いてＥＬＩＳＡ法により測定した。イラクサ抽出物のＴＧＦ−β産生促進効果は、ＤＭＥＭ培地のみで培養した場合のＴＧＦ−β量を１００とした場合の値（比率）で示した。 After culturing for 3 days, the culture supernatant was collected, and the amount of TGF-β contained in the culture supernatant was measured by ELISA using a human TGF-β Immunoassay kit (manufactured by GT). The effect of promoting the TGF-β production of the extract of nettle was shown as a value (ratio) when the amount of TGF-β when cultured in only the DMEM medium was 100.
その結果を表１に示す。 The results are shown in Table 1.
表１からも明らかなように、イラクサ抽出物を含むＤＭＥＭ培地で培養したヒト毛乳頭細胞から産生されたＴＧＦ−β量は、イラクサ抽出物を含まないＤＭＥＭ培地（コントロール）で培養したヒト毛乳頭細胞から産生されたＴＧＦ−β量よりも増加していた。このことから、イラクサ抽出物は毛乳頭細胞によるＴＧＦ−β産生を促進するものと認められた。 As is apparent from Table 1, the amount of TGF-β produced from human hair papilla cells cultured in DMEM medium containing nettle extract is equal to that of human hair papilla cultured in DMEM medium (control) not containing nettle extract. There was an increase over the amount of TGF-β produced from the cells. From this, it was confirmed that the nettle extract promotes TGF-β production by hair papilla cells.
また、イラクサ抽出物の濃度が高くなる程、産生されるＴＧＦ−β量が増加し、イラクサ抽出物の濃度を高くすることで、ＴＧＦ−βの産生促進効果が高まることがわかった。 Moreover, it turned out that the amount of TGF- (beta) produced increases, and the production promotion effect of TGF- (beta) increases by making the density | concentration of a nettle extract high, so that the density | concentration of a nettle extract becomes high.
（試験例２）マウスを用いた毛成長抑制作用試験
Ｃ３Ｈ／Ｈｅマウス（８週齢、雄）の背部を剃毛し、１日に１回、０．０１重量％のイラクサ抽出物を含む５０％エタノール溶液を０．１ｍｌずつ、２０日間塗布した。一方、イラクサ抽出物を含まない５０％エタノール溶液を、同様にＣ３Ｈ／Ｈｅマウスの背部に１日に１回塗布し、これを対照群とした。イラクサ抽出物の毛成長抑制効果は、剃毛後２０日目における剃毛部の毛再生面積率を比較することにより調べた。
Test Example 2 Hair Growth Inhibitory Action Test Using Mice 50 C3H / He mice (8 weeks old, male) were shaved and shaved once each day, containing 0.01% by weight of nettle extract 50 A 0.1% ethanol solution was applied in 0.1 ml portions for 20 days. On the other hand, a 50% ethanol solution containing no nettle extract was similarly applied once a day to the back of C3H / He mice, and this was used as a control group. The hair growth inhibitory effect of the nettle extract was examined by comparing the hair regeneration area ratio of the shaved part on the 20th day after shaving.
その結果を表２に示す。 The results are shown in Table 2.
表２からも明らかなように、対照群では毛再生面積が８６％であったのに対し、０．０１％のイラクサ抽出物を含む５０％エタノール溶液を塗布したＣ３Ｈ／Ｈｅマウス（イラクサ抽出物塗布群）では毛再生面積が３２％で、対照群に比べて著しく毛再生面積が小さかった。このことから、イラクサ抽出物はマウスの毛成長の抑制効果が非常に良好であると認められた。 As apparent from Table 2, the hair regeneration area in the control group was 86%, whereas C3H / He mice (nettle extract) coated with a 50% ethanol solution containing 0.01% nettle extract were used. In the application group), the hair regeneration area was 32%, and the hair regeneration area was significantly smaller than that of the control group. From this, it was recognized that the nettle extract has a very good inhibitory effect on hair growth in mice.
（処方例１）
本処方例は、化粧料の一例としてのスキンケアローションの処方例であり、その組成は次のとおりである。
(Prescription Example 1)
This prescription example is a prescription example of a skin care lotion as an example of a cosmetic, and its composition is as follows.
配合成分 重量％
エタノール ５．０
１，３−ブチレングリコール ７．０
ソルビット液 １０．０
イラクサ抽出物 ０．０１
パラオキシ安息香酸メチル ０．１５
クエン酸 ０．０５
クエン酸ナトリウム ０．２
エデト酸塩 ０．０５
香料 ０．１
ＰＯＥ硬化ヒマシ油 ０．５
精製水 残量
Compounding ingredients Weight%
Ethanol 5.0
1,3-butylene glycol 7.0
Sorbit solution 10.0
Nettle extract 0.01
Methyl paraoxybenzoate 0.15
Citric acid 0.05
Sodium citrate 0.2
Edetate 0.05
Fragrance 0.1
POE hydrogenated castor oil 0.5
Purified water remaining
（処方例２）
本処方例は、化粧料の一例としてのスキンケアクリームの処方例であり、その組成は次のとおりである。
配合成分 重量％
セタノール ２．５
スクワラン １０．０
サラシミツロウ １．０
トリオクタン酸グリセリル ５．０
ミリスチン酸オクチルドデシル １５．０
酢酸トコフェロール ０．１
１，３−ブチレングリコール ７．０
モノステアリン酸グリセリン ３．０
ＰＯＥ（２０）ソルビタンモノステアレート １．０
ソルビタンモノステアレート ２．０
イラクサ抽出物 ０．１
濃グリセリン ５．０
パラオキシ安息香酸ブチル ０．１
パラオキシ安息香酸メチル ０．２
精製水 残量
(Prescription example 2)
This prescription example is a prescription example of a skin care cream as an example of a cosmetic, and its composition is as follows.
Compounding ingredients Weight%
Cetanol 2.5
Squalane 10.0
Sara honey bee 1.0
Glyceryl trioctanoate 5.0
Octyldodecyl myristate 15.0
Tocopherol acetate 0.1
1,3-butylene glycol 7.0
Glycerol monostearate 3.0
POE (20) sorbitan monostearate 1.0
Sorbitan monostearate 2.0
Nettle extract 0.1
Concentrated glycerin 5.0
Butyl paraoxybenzoate 0.1
Methyl paraoxybenzoate 0.2
Purified water remaining
本発明の体毛の毛成長抑制のためのＴＧＦ−β産生促進剤は、皮膚外用剤、化粧料等に広く適用することができる。 The TGF-β production promoter for suppressing hair growth of body hair of the present invention can be widely applied to external preparations for skin, cosmetics and the like.
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