JP2012217398A - Method for producing oyster meat extract containing large amount of antioxidant having high antioxidative activity - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a method for producing an oyster meat extract containing a large amount of antioxidant having high antioxidative activity by selecting a portion of an oyster meat extract containing a large amount of antioxidant having high antioxidative activity, and adopting an extraction method by which the large amount of antioxidant having high antioxidative activity can be obtained, thereby attaining efficient extraction of a large amount of useful oyster meat extract containing the antioxidant having extremely high antioxidative activity, to produce the oyster meat extract containing the large amount of antioxidant having high antioxidative activity.SOLUTION: The method includes storing oyster meat 3 in an extraction container 2 containing an aqueous solution 1, extracting the oyster meat extract from the oyster meat 3 to produce a liquid extract, pouring the produced liquid 7 into a centrifugal separator, and rotating the centrifugal separator at a centrifugal acceleration so as to separate and take out the antioxidant with high antioxidative activity, thus acquiring the oyster meat extract containing the large amount of antioxidant having high antioxidative activity after separation.

Description

For example, the present invention efficiently extracts an extract of oyster meat containing a large amount of an antioxidant substance having a high antioxidant power from oyster meat such as raw oysters, and has the above-mentioned high antioxidant power existing in the oyster meat extract The present invention relates to a method for producing an anti-oxidant-rich oyster meat extract having a high anti-oxidant power that can be extracted and contained in a large amount without missing a substance.

  In recent years, the perception of oyster meat extract as a health supplement is an extremely excellent product that contains a lot of beneficial substances.

  At present, for example, health supplements relating to oyster meat extract extracted by a variety of extraction methods are sold (Japanese Patent Laid-Open No. 10-136946).

  In particular, taurine, zinc, and selenium are essential trace elements for humans, but they are easily lost even among the mineral components that are insufficient for ingestion.

  Diabetes is a disease in which the hormone `` insulin '' that lowers blood sugar weakens, or the sugar content in the blood becomes abnormally high due to insufficient insulin. Minerals such as zinc and selenium are helping. As these minerals are said to have "insulin action", it is recognized that blood glucose levels are lowered when these minerals are actually supplemented to diabetic patients. And many of these active ingredients are contained in oyster meat.

  Therefore, when extracting the extract of oyster meat containing a large amount of the above-mentioned active ingredients, it is rich in beneficial substances such as vitamins, taurine, glycogen, and proteins that are good for the body of the human body, such as zinc and selenium. It is desirable to efficiently extract a oyster meat extract that is contained in a well-balanced manner, and to produce a good oyster meat extract. The inventor has invented methods for producing an oyster meat extract in line with those requests, and has obtained a patent. I have done it.

  Furthermore, in recent years, attention has been drawn to the fact that oyster meat contains a large amount of antioxidants having so-called high antioxidant performance. And the fact became more and more clear through various researches and experiments by the present inventor, and the development of a method for producing a oyster meat extract containing more antioxidant substances having a particularly high antioxidant power has been strongly demanded.

  Therefore, the present inventor has recently conducted research on the oyster meat portion that contains a large amount of the antioxidant substance having a high antioxidant power and the extraction method that contains a large amount of the antioxidant substance having a high antioxidant power. A production method that can efficiently extract oyster meat extract that contains a lot of powerful antioxidants, that is, it can extract and produce oyster meat extract containing a large amount of antioxidants with high antioxidant power in an optimal way Has continued his invention creation activities.

  By the way, the generation of so-called active oxygen is caused by aerobic life, which causes oxidation of lipids, proteins, and nucleic acids and damages cells. Normally, the level of oxidation in the living body is almost constant by the balance between the active oxygen production system and the elimination system by antioxidants, but this balance is lost due to various factors such as drugs, radiation, and ischemia, and active oxygen production Leaning toward the system is said to be oxidative stress.

  This accumulation of oxidative stress is thought to contribute to various diseases and aging such as cancer, arteriosclerotic disease, ischemia / reperfusion injury, rheumatoid arthritis, diabetes, Alzheimer's disease and Parkinson's disease neuropathy It has been.

Oysters, such as oysters (Crassostreagigas), are bivalves belonging to the order of the genus Coleoptera, and their habitat extends throughout Japan and other parts of East Asia. In recent years, oysters have been cultivated in France and Australia and are famous as the most edible oysters in the world. Oysters have been edible since ancient times due to their high nutritional value, but as described above, oyster meat extracts extracted from oyster meat include glycogen and protein, as well as minerals such as calcium, zinc, selenium, copper, and manganese. In addition to a large amount of the above, it also contains a large amount of the antioxidant substance having a high antioxidant power.

Japanese Patent Laid-Open No. 10-136946

Thus, the present invention was devised in view of the above-mentioned conventional demands, and a portion of oyster meat containing a large amount of antioxidant substances having so-called high antioxidant power, in other words, an antioxidant substance having high antioxidant power. In addition to selecting a portion of the oyster meat extract that contains a large amount of the extract, an extraction method that can contain a large amount of antioxidants having a high anti-oxidant power is adopted for the selected extract so that it has an extremely high anti-oxidant power Antioxidant with high antioxidant ability that can extract beneficial oyster meat extract containing high antioxidants efficiently and in large quantities, and can produce oyster meat extract with high antioxidant substances An object of the present invention is to provide a method for producing a substance-rich oyster meat extract.

The method for producing a oyster meat extract containing a large amount of an antioxidant substance having high antioxidant power according to the present invention,
Storing oyster meat in an extraction container in which an aqueous solution is stored, extracting oyster meat extract from oyster meat in the extraction container to produce an extract,
The liquid after the production is injected into a centrifuge, and the centrifuge is rotated at a centrifugal acceleration that causes an antioxidant substance having a high antioxidant power to be separated from the liquid and removed. Isolates the power antioxidants,
Obtain oyster meat extract containing a lot of antioxidants with high antioxidant power after separation,
It is characterized by
Or
The oyster meat was stored in the extraction container in which the aqueous solution was stored, the oyster meat extract was extracted from the oyster meat in the extraction container to produce an extract, the oyster meat was taken out after the extraction, and the oyster meat was taken out Concentrate the later extract to produce a concentrate, add ethanol to the concentrate, stir to separate the precipitate and supernatant, take out the supernatant after separation,
The extracted supernatant is poured into a centrifuge, and the centrifuge is rotated at a centrifugal acceleration at which a high-antioxidant power antioxidant in the supernatant is separated and removed, and the injected supernatant is Separating the antioxidant substance with high antioxidant power from inside,
Obtain oyster meat extract containing a lot of antioxidants with high antioxidant power after separation,
It is characterized by
Or
The oyster meat was stored in the extraction container in which the aqueous solution was stored, the oyster meat extract was extracted from the oyster meat in the extraction container to produce an extract, the oyster meat was taken out after the extraction, and the oyster meat was taken out Concentrate the later extract to produce a concentrate, add ethanol to the concentrate, stir to separate the precipitate and supernatant, take out the supernatant after separation,
The extracted supernatant is poured into a centrifuge, and the centrifuge is rotated at a centrifugal acceleration at which a high-antioxidant power antioxidant in the supernatant is separated and removed, and the injected supernatant is Separating the antioxidant substance of high antioxidant power from the inside as a centrifugal supernatant,
The oyster meat extract containing a large amount of an antioxidant having a high antioxidant power in the centrifugal supernatant was collected,
It is characterized by
Or
The oyster meat was stored in the extraction container in which the aqueous solution was stored, the oyster meat extract was extracted from the oyster meat in the extraction container to produce an extract, the oyster meat was taken out after the extraction, and the oyster meat was taken out Concentrate the later extract to produce a concentrate, add ethanol to the concentrate, stir to separate the precipitate and supernatant, take out the supernatant after separation,
The extracted supernatant is injected into a centrifuge, and the centrifuge is rotated so as to obtain a centrifugal acceleration exceeding the diffusivity of a substance having no antioxidant power or a substance having a low antioxidant power in the supernatant. Rotate by a few and separate the substance with no anti-oxidative power or low anti-oxidant substance from the injected supernatant into the centrifugal sediment and separate it into the centrifugal supernatant and the centrifugal sediment And
The centrifugal supernatant after separation contains a large amount of an antioxidant substance having a high antioxidant power,
It is characterized by
Or
The oyster meat was stored in the extraction container in which the aqueous solution was stored, the oyster meat extract was extracted from the oyster meat in the extraction container to produce an extract, the oyster meat was taken out after the extraction, and the oyster meat was taken out Concentrate the later extract to produce a concentrate, add ethanol to the concentrate, stir to separate the precipitate and supernatant, take out the supernatant after separation,
The extracted supernatant is continuously injected into a continuous centrifuge, and the continuous centrifuge is centrifuged at a concentration exceeding the diffusivity of a substance having no antioxidant power or a substance having a low antioxidant power in the supernatant. Rotate at a rotational speed that provides acceleration, separate the non-antioxidant substance or low-antioxidant substance from the injected supernatant into the centrifugal sediment, and centrifuge the supernatant and centrifuge Separated into deposits,
The centrifugal supernatant after separation contains a large amount of an antioxidant substance having a high antioxidant power,
It is characterized by
Or
The oyster meat was stored in the extraction container in which the aqueous solution was stored, the oyster meat extract was extracted from the oyster meat in the extraction container to produce an extract, the oyster meat was taken out after the extraction, and the oyster meat was taken out Concentrate the later extract to produce a concentrate, add ethanol to the concentrate until the ethanol concentration is 30% to 90%, preferably 70%, and stir to separate the precipitate and the supernatant. And after separation, the supernatant is removed,
The extracted supernatant is continuously injected into a continuous centrifuge, and the continuous centrifuge is centrifuged at a concentration exceeding the diffusivity of a substance having no antioxidant power or a substance having a low antioxidant power in the supernatant. Rotate at a rotational speed that provides acceleration, separate the non-antioxidant substance or low-antioxidant substance from the injected supernatant into the centrifugal sediment, and centrifuge the supernatant and centrifuge Separated into deposits,
The centrifugal supernatant after separation contains a large amount of an antioxidant substance having a high antioxidant power,
It is characterized by
Or
The centrifugal acceleration of the continuous centrifuge that exceeds the diffusive power of the substance having no antioxidant power or the low antioxidant power in the supernatant is approximately 8000 × G.
It is characterized by
Or
The oyster meat is stripped,
It is characterized by this.

If the method for producing oyster meat extract containing a large amount of antioxidant substance having high antioxidant power according to the present invention,
Select the portion of the oyster meat that contains a lot of antioxidants with high antioxidant power, in other words, the portion of the oyster meat extract that contains a lot of antioxidants with high antioxidant power, and the selected extraction By using an extraction method that can contain a large amount of antioxidants with high antioxidant power, oyster meat extract that contains a high amount of antioxidants with high antioxidant power can be extracted efficiently and in large quantities, so that high antioxidant It has an excellent effect that it can produce a oyster meat extract containing a large amount of powerful antioxidants.

BRIEF DESCRIPTION OF THE DRAWINGS It is schematic structure explanatory drawing (the 1) which shows the antioxidant material-rich oyster meat extract manufacturing method which has high antioxidant power by this invention. It is explanatory drawing explaining schematic structure of the continuous centrifuge by this invention. It is explanatory drawing explaining schematic structure of the continuous centrifuge by another Example. It is explanatory drawing explaining the measurement principle of ORAC method. It is explanatory drawing explaining the apparatus, reagent, and unit which are used by the calculation method of ORAC value and ORAC method. It is explanatory drawing explaining the ORAC value of various foodstuffs. It is explanatory drawing which shows the example of calculation of the maximum centrifugal acceleration.

  Hereinafter, preferred embodiments of the present invention will be described.

  First, the oyster meat 3 is put in the extraction container 2 in which the aqueous solution 1 is stored in order to extract the oyster meat extract.

  Here, the type of the aqueous solution 1 used for extraction is not limited at all, but water may generally be used. Also, the temperature of the water is not limited in any way, and it may be ordinary room temperature water, 30 ° C. to 50 ° C. hot water, or hot water of 50 ° C. or higher. Moreover, ethanol may be mixed into the water to form an ethanol solution. Extraction of oyster meat extract in ethanol solution can be promoted by mixing ethanol.

  In the extraction, the inside of the extraction container 2 may be performed at a normal pressure, or the inside of the extraction container 2 may be sealed and the pressure may be reduced to 1 atm or lower or pressurized to 1 atm or higher.

  This is because a good extraction method is selected so as to contain a large amount of an antioxidant substance having a high antioxidant power.

  Next, after a predetermined time, for example, several hours of extraction time has elapsed, the oyster meat 3 is taken out from the extraction container 2 and the extracted liquid in the extraction container 2 is concentrated to produce a concentrated liquid 4.

  Various concentration methods exist for this concentration method, but the present invention is not limited to this, and any concentration method may be used. A so-called low temperature heating concentration method or a high temperature heating concentration method may be used.

  Further, the concentration ratio of the concentrated liquid 4 is not limited at all, and it may be concentrated to 1/3 or 1/2.

  Next, ethanol 5 is added to the concentrated solution 4 so that the ethanol concentration is about 30% to 80%, and preferably the ethanol concentration is 70%. Stir and separate into precipitate 6 and supernatant 7. This separation method is not limited in any way, but in the natural separation method by natural precipitation, after stirring, it waits for a predetermined time and waits for the precipitate 6 to settle naturally.

  After the precipitate 6 has settled and the precipitate 6 and the supernatant liquid 7 are clearly separated, only the supernatant liquid 7 is taken out.

And it is comprised so that this supernatant liquid 7 can be continuously inject | poured into the continuous centrifuge 8 from the injection pipe 9 of the continuous centrifuge 8 shown in FIG.
As shown in FIG. 3, the supernatant 7 is injected from the left injection pipe 10 and ethanol 5 is injected from the right injection pipe 11, and the centrifugal action of the continuous centrifuge 8 is performed by the action of the ethanol 5. However, it may be configured so as to facilitate separation and further separation.

  Here, injection of a predetermined amount of the supernatant 7 into the continuous centrifuge 8 is confirmed, and the continuous centrifuge 8 is operated, so that the so-called centrifuge precipitate 15 and the centrifuge supernatant 16 are continuously added. To be separated.

  As can be seen from the figure, the so-called centrifugal sediment 15 is collected by centrifugal force on the outer peripheral side wall 13 side of the storage tank 12, and the other is sent from the upper side of the storage tank 12 to the outside through the delivery pipe 14. What is called is the so-called centrifugal supernatant 16.

  In this embodiment, as is understood from FIG. 1, for example, the concentrated liquid 4 to which ethanol 5 has been added so as to have a concentration of 70% is stirred, and thereafter waiting for spontaneous precipitation. For example, 30 liters of the supernatant 7 collected after the natural precipitation is continuously injected into the continuous centrifuge 8.

  Then, the continuous centrifuge 8 is operated, and the supernatant liquid 7 is continuously centrifuged.

  As a result, it was confirmed that 0.3 liters of so-called centrifugal precipitate 15 and 29.7 liters of continuous centrifugal supernatant 16 were collected.

  In the present invention, it has been confirmed that the continuous centrifugal supernatant 16 contains a large amount of an antioxidant substance having a high antioxidant power.

  Here, the numerical value indicating the antioxidant power by the so-called ORAC method (Oxygen Radical Absorbance Capacity method), that is, the ORAC value, indicates how much the substance having the antioxidant power is contained in the centrifugal supernatant 16. Was detected. As a result, as described above, it was confirmed that an antioxidant substance having an extremely high antioxidant power was contained.

  The measurement principle of the ORAC method will be explained briefly. First, when a certain reactive oxygen species is generated, the fluorescence intensity decomposed thereby is measured, and a fluorescence intensity curve that decreases with time is drawn, if an antioxidant coexists in this reaction system, the fluorescence of the fluorescent substance The rate of decrease in intensity is delayed. Therefore, the existence of antioxidant substances can be confirmed by this principle.

Based on the above principle, referring to FIG. 4, the area under the curve of the fluorescence intensity in the presence of the specimen or standard substance (AUC:
Calculate the difference (net AUC) between the area under the curve and the AUC in the absence (blank).
Find the relative value to AUC. Based on the relative value, it is converted into Trolox concentration to obtain the antioxidant power of the specimen (unit: μmol TE / g: micromol Trolox equivalent / gram).

  The method for calculating the ORAC value is shown in FIG.

That is, in the ORAC method, first, a fluorescent probe (Fluorescein) is added to a sample solution or a standard solution (Trolox), and AAPH (2,2'-Azobis (2-amidinopropane) is used as a radical initiator.
When active oxygen is generated using dihydrochloride), fluorescein is oxidized by active oxygen.

  Since the fluorescein oxide does not have fluorescence, the fluorescence intensity decreases with time. When the sample has antioxidant power, the active oxygen is erased by the antioxidant and the oxidation of Fluorescein is suppressed. Therefore, the fluorescence intensity of Fluorescein persists and decreases at the rate when no antioxidant is present (blank). Is delayed.

The fluorescence intensity of the sample or Trolox and blank is plotted on the vertical axis, and the measurement time is plotted on the horizontal axis. The area under the curve of the fluorescence intensity of the sample solution or Trolox (Area
Under the Curve (AUCsample or AUCtrolox) and blank area under the curve (AUCblank), that is, the area of the shaded area is calculated (netAUCsample and netAUCTrolox, respectively) The concentration is obtained and, for example, the ORAC value is calculated as the number of micromoles of Trolox per 1 g of sample.

Therefore, the unit of ORAC value is μmole TE / g (TE: Trolox
Equivalent) etc. will be used.

  Here, it should be noted that the ORAC value expresses the antioxidant power in terms of the amount of the standard substance (Trolox) and is not a value indicating a specific antioxidant mass. However, when the ORAC value is high, for example, it can be understood that the oyster meat extract contains an antioxidant substance having a high antioxidant power.

Thus, in this example, when the ORAC value for the continuous centrifugal supernatant 16 was detected, the value was 160 μmole.
A value of TE / g was detected, which was extremely high in the normal food group.

USA ORAC has a database of ORAC values for various foods. The graph is shown in FIG. As can be understood from FIG. 6, even the blueberry that the United States ORAC recognizes as a high value has an ORAC value of 66.2 μmole.
TE / g.

In contrast, the oyster meat extract of this example, that is, the continuous centrifugal supernatant 16 has a value of 160 μmole, which is considered to have about two times higher antioxidant power than this.
It can be understood that an antioxidant having a TE / g ORAC value is contained.

The inventor added oyster meat extract before separation by the continuous centrifuge 8 in the present invention, that is, as shown in FIG. The ORAC value is also detected for the solution in the concentration ethanol solution. As a result, the 70% ethanol solution was 132 μmole.
A TE / g ORAC value was detected.

Further, as described above, 30 liters of the ethanol solution was applied to the continuous centrifuge 8 as described above, and 29.7 liters of the supernatant supernatant 16 and 0.3 liters of the centrifugal precipitate 15 were obtained. And the ORAC value of the continuous centrifugation supernatant 16 is 160 μmole as described above.
On the other hand, the ORAC value of the continuous centrifugal precipitate 15 was 30.7 μmole TE / g.

  Considering this reason, the continuous centrifuge 8 rotates with a strong rotational force, and centrifugal acceleration higher than normal gravity is applied to the injected supernatant liquid 7.

  As a result, forced separation is performed by this centrifugal acceleration. Then, it is considered that a substance that never separates and precipitates by natural precipitation, that is, a substance that does not have the antioxidant power of an antioxidant substance or a substance that has a low antioxidant power is forcibly separated. This is because these substances are understood to be contained in the continuous centrifugal precipitate 15 due to strong centrifugal acceleration.

  That is, so-called fine particles that have not settled only by normal gravity, such as natural precipitation, such as a substance that does not have the antioxidant power of an antioxidant substance or a substance that has a low antioxidant power, diffuses the diffusive power of the continuous centrifugation. The centrifugal acceleration due to the rotation of the separator 8 is increased, and for example, a substance having no antioxidant power or a substance having a low antioxidant power is forcibly separated to the centrifugal precipitate 15 side.

  When the continuous centrifuge 8 is operated and rotated, a centrifugal acceleration acts on the supernatant liquid 7 injected into the storage tank 12, and this centrifugal acceleration is, for example, a substance that does not have an antioxidant power or an antioxidant power. When a substance with a low concentration becomes larger than the diffusive power to be diffused, the natural precipitate does not precipitate, for example, a substance having no antioxidant power or a substance with low antioxidant power is separated and centrifuged. This is because the separated precipitate 15 is obtained.

Therefore, the ORAC value of the solution of the concentrated solution to which so-called ethanol is added before separation with a continuous centrifuge is 132 μmole.
Compared to TE / g, the ORAC value of the supernatant produced by forced separation with a continuous centrifuge is as high as 160 μmole TE / g, which makes it highly resistant to antioxidants. It became possible to extract, obtain, and manufacture oyster meat extract that incorporated a large amount of oxidizing substances.

  Note that, for the continuous centrifuge 8, for example, the number of revolutions at which centrifugal acceleration exceeding the diffusive force of the substance that does not have the antioxidant power of the antioxidant substance or the substance with low antioxidant power is present in the supernatant liquid 7 is obtained. In consideration, as can be understood from FIG. 7, when the maximum rotation radius of the continuous centrifugal separator 8 is 11.4 cm and the maximum rotation speed is 8000 rpm, the maximum centrifugal acceleration is a numerical value of approximately 8157 × g.

Therefore, in the present invention, the maximum centrifugal acceleration of the continuous centrifuge 8 is a numerical value of approximately 8157 × g, and the centrifugal acceleration of this numerical value inhibits the antioxidant power of the antioxidant present in the supernatant liquid 7. First of all, it was proved that it could be said to be a centrifugal acceleration exceeding the diffusive power of the inhibitor (particle).

1 Aqueous solution 2 Extraction vessel 3 Oyster meat 4 Concentrate 5 Ethanol
6 Precipitate 7 Supernatant 8 Continuous centrifuge 9 Injection pipe 10 Left delivery pipe 11 Right delivery pipe 12 Storage tank 13 Outer peripheral wall 14 Delivery pipe 15 Centrifugal sediment 16 Centrifugal supernatant

Claims (8)

Storing oyster meat in an extraction container in which an aqueous solution is stored, extracting oyster meat extract from oyster meat in the extraction container to produce an extract,
The liquid after the production is injected into a centrifuge, and the centrifuge is rotated at a centrifugal acceleration that causes an antioxidant substance having a high antioxidant power to be separated from the liquid and removed. Isolates the power antioxidants,
Obtain oyster meat extract containing a lot of antioxidants with high antioxidant power after separation,
A method for producing an oyster meat extract containing a high antioxidant substance and having a high antioxidant power.
The oyster meat was stored in the extraction container in which the aqueous solution was stored, the oyster meat extract was extracted from the oyster meat in the extraction container to produce an extract, the oyster meat was taken out after the extraction, and the oyster meat was taken out Concentrate the later extract to produce a concentrate, add ethanol to the concentrate, stir to separate the precipitate and supernatant, take out the supernatant after separation,
The extracted supernatant is poured into a centrifuge, and the centrifuge is rotated at a centrifugal acceleration at which a high-antioxidant power antioxidant in the supernatant is separated and removed, and the injected supernatant is Separating the antioxidant substance with high antioxidant power from inside,
Obtain oyster meat extract containing a lot of antioxidants with high antioxidant power after separation,
A method for producing an oyster meat extract containing a high antioxidant substance and having a high antioxidant power.
The oyster meat was stored in the extraction container in which the aqueous solution was stored, the oyster meat extract was extracted from the oyster meat in the extraction container to produce an extract, the oyster meat was taken out after the extraction, and the oyster meat was taken out Concentrate the later extract to produce a concentrate, add ethanol to the concentrate, stir to separate the precipitate and supernatant, take out the supernatant after separation,
The extracted supernatant is poured into a centrifuge, and the centrifuge is rotated at a centrifugal acceleration at which a high-antioxidant power antioxidant in the supernatant is separated and removed, and the injected supernatant is Separating the antioxidant substance of high antioxidant power from the inside as a centrifugal supernatant,
The oyster meat extract containing a large amount of an antioxidant having a high antioxidant power in the centrifugal supernatant was collected,
A method for producing an oyster meat extract containing a high antioxidant substance and having a high antioxidant power.
The oyster meat was stored in the extraction container in which the aqueous solution was stored, the oyster meat extract was extracted from the oyster meat in the extraction container to produce an extract, the oyster meat was taken out after the extraction, and the oyster meat was taken out Concentrate the later extract to produce a concentrate, add ethanol to the concentrate, stir to separate the precipitate and supernatant, take out the supernatant after separation,
The extracted supernatant is injected into a centrifuge, and the centrifuge is rotated so as to obtain a centrifugal acceleration exceeding the diffusivity of a substance having no antioxidant power or a substance having a low antioxidant power in the supernatant. Rotate by a few and separate the substance with no anti-oxidative power or low anti-oxidant substance from the injected supernatant into the centrifugal sediment and separate it into the centrifugal supernatant and the centrifugal sediment And
The centrifugal supernatant after separation contains a large amount of an antioxidant substance having a high antioxidant power,
A method for producing an oyster meat extract containing a high antioxidant substance and having a high antioxidant power.
The oyster meat was stored in the extraction container in which the aqueous solution was stored, the oyster meat extract was extracted from the oyster meat in the extraction container to produce an extract, the oyster meat was taken out after the extraction, and the oyster meat was taken out Concentrate the later extract to produce a concentrate, add ethanol to the concentrate, stir to separate the precipitate and supernatant, take out the supernatant after separation,
The extracted supernatant is continuously injected into a continuous centrifuge, and the continuous centrifuge is centrifuged at a concentration exceeding the diffusivity of a substance having no antioxidant power or a substance having a low antioxidant power in the supernatant. Rotate at a rotational speed that provides acceleration, separate the non-antioxidant substance or low-antioxidant substance from the injected supernatant into the centrifugal sediment, and centrifuge the supernatant and centrifuge Separated into deposits,
The centrifugal supernatant after separation contains a large amount of an antioxidant substance having a high antioxidant power,
A method for producing an oyster meat extract containing a high antioxidant substance and having a high antioxidant power.
The oyster meat was stored in the extraction container in which the aqueous solution was stored, the oyster meat extract was extracted from the oyster meat in the extraction container to produce an extract, the oyster meat was taken out after the extraction, and the oyster meat was taken out Concentrate the later extract to produce a concentrate, add ethanol to the concentrate until the ethanol concentration is 30% to 90%, preferably 70%, and stir to separate the precipitate and the supernatant. And after separation, the supernatant is removed,
The extracted supernatant is continuously injected into a continuous centrifuge, and the continuous centrifuge is centrifuged at a concentration exceeding the diffusivity of a substance having no antioxidant power or a substance having a low antioxidant power in the supernatant. Rotate at a rotational speed that provides acceleration, separate the non-antioxidant substance or low-antioxidant substance from the injected supernatant into the centrifugal sediment, and centrifuge the supernatant and centrifuge Separated into deposits,
The centrifugal supernatant after separation contains a large amount of an antioxidant substance having a high antioxidant power,
A method for producing an oyster meat extract containing a high antioxidant substance and having a high antioxidant power.
The centrifugal acceleration of the continuous centrifuge that exceeds the diffusive power of the substance having no antioxidant power or the low antioxidant power in the supernatant is approximately 8000 × G.
The method for producing a oyster meat extract containing a large amount of an antioxidant substance having a high antioxidant power according to claim 4, 5, or 6.
The oyster meat is stripped,
The production of oyster meat extract containing a high antioxidant power and containing an antioxidant substance according to claim 1, claim 2, claim 3, claim 4, claim 5, claim 6 or claim 7. Method

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