JP4716523B2 - Production method of oyster meat extract with adenosine - Google Patents

Description

  The present invention relates to a method for producing an adenosine-containing oyster meat extract, which produces an adenosine-containing oyster meat extract from an oyster meat extract such as raw oysters.

Adenosine is one of nucleosides consisting of adenine and ribose. The adenine and ribose are β-N9-glycosidically linked, and the molecular weight (C10H13N5O4) is indicated as 267.
Here, adenosine plays an important role in living organisms, and is used as a base for DNA and RNA in the coding of genetic information, and is also involved in energy transport as part of ATP and ADP in biochemical processes. It is involved in signal transduction as a cyclic AMP and thus plays an important role in vivo.
Adenosine is a degradation product of adenosine triphosphate (ATP) and acts as one of the major local function control factors in many organs.
For example, the cardiovascular system is also responsible for a variety of functions in physiological and pathological conditions, such as cell differentiation, angiogenesis, coronary blood flow, stimulation conduction system, metabolism, and sensitivity to sympathetic nerves. It plays an important role in acquiring myocardial ischemia tolerance during blood.

In recent years, the perception of oyster meat extract is generally increasing day by day as an excellent product containing many various beneficial substances as health supplements.
At present, for example, health supplements relating to oyster meat extract extracted by a variety of extraction methods are sold (Japanese Patent Laid-Open No. 10-136946).

  Thus, when extracting such oyster meat extract, it contains a large amount of a substance having an action to suppress aggregation of so-called platelets including beneficial nutrients such as glycogen, taurine or protein, and zinc, etc. Although it was considered desirable to extract the extract in an extract, the extraction of the oyster meat containing adenosine has not been proposed in the past, and in recent years the importance of adenosine has increased as described above. Therefore, extraction and production methods for efficiently and abundantly extracting adenosine-containing oyster meat extract have been demanded.

This is because if an extract of oyster meat rich in adenosine can be obtained from raw oyster meat, health foods having different active ingredients from the conventional oyster meat extract product can be provided to the market.
Japanese Patent Laid-Open No. 10-136946

  Thus, the present invention has been devised in view of the above problems, and adenosine can be extracted from raw oyster meat by a production method that can efficiently extract and produce adenosine-containing oyster meat extract from raw oyster, and can be produced by taking out abundantly. An object of the present invention is to provide an adenosine-containing health food containing different active ingredients from a conventional oyster meat extract product.

The present invention
Raw oyster meat is stored in an extraction container, an ethanol solution is stored in the extraction container, and the oyster meat extract is extracted with ethanol.
Concentrate the extracted oyster meat extract,
Add concentrated oyster meat extract in ethanol solution and stir, separate into natural precipitate and supernatant,
Taking out the natural precipitate and the supernatant obtained after the stirring and separation,
Remove and concentrate the removed supernatant until it becomes paste-like or bowl-like,
Add the paste-like or bowl-like concentrate again into the ethanol solution, then stir and separate, dissolve the adenosine present in the oyster meat extract concentrate in the ethanol solution in the supernatant after separation, and dissolve the solution The ethanol solution was removed from the liquid and the oyster meat extract with adenosine was taken out.
It is characterized by
Or
Raw oyster meat is stored in an extraction container, an ethanol solution is stored in the extraction container, and the oyster meat extract is extracted with ethanol.
Concentrate the extracted oyster meat extract,
Add concentrated oyster meat extract in ethanol solution and stir, separate into natural precipitate and supernatant,
Taking out the natural precipitate and the supernatant obtained after the stirring and separation,
Remove and concentrate the removed supernatant until it becomes paste-like or bowl-like,
Add the paste-like or bowl-like concentrated solution again into the ethanol solution to make an ethanol solution with a concentration of 20% to 90%, then stir and separate, and the oyster meat extract in the ethanol solution in the separated supernatant. Adenosine present in the concentrate was dissolved, the ethanol solution was removed from the solution, and the oyster meat extract containing adenosine was taken out.
It is characterized by
Or
Raw oyster meat is stored in an extraction container, an ethanol solution is stored in the extraction container, and the oyster meat extract is extracted with ethanol.
Concentrate the extracted oyster meat extract,
Add concentrated oyster meat extract in ethanol solution and stir, separate into natural precipitate and supernatant,
Taking out the natural precipitate and the supernatant obtained after the stirring and separation,
Remove and concentrate the removed supernatant until it becomes paste-like or bowl-like,
Add the paste-like or bowl-like concentrate again into the ethanol solution to make a 70% ethanol solution, then stir and separate, and separate the supernatant into the ethanol solution into the oyster meat extract concentrate. The existing adenosine was dissolved, the ethanol solution was removed from the solution, and the oyster meat extract containing adenosine was taken out.
It is characterized by
Or
Of the stirring, at least the last stirring is performed by a continuous centrifugal separator.
It is characterized by
Or
The pressure at the time of extraction of the oyster meat extract is performed by selecting either reduced pressure or increased pressure, or normal pressure,
It is characterized by
Or
Extraction of the oyster meat extract was performed a second time from the oyster meat from which the extract was extracted once, and the first extract and the second extract were mixed to form an extract.
It is characterized by
Or
Concentration of the extracted oyster meat extract was concentrated by heating under reduced pressure and low temperature,
It is characterized by
Or
Concentration of the removed supernatant was concentrated by heating under reduced pressure and low temperature.
It is characterized by
Or
The raw oyster meat is stripped,
It is characterized by this.

According to the method for producing an adenosine-containing oyster meat extract according to the present invention, adenosine is abundant in raw oyster meat by a production method that can efficiently extract and produce an adenosine-containing oyster meat extract from raw oysters. The oyster meat extract contained in the oyster meat is obtained, so that it is possible to provide an adenosine-containing health food with different active ingredients from the conventional oyster meat extract product.

Hereinafter, the present invention will be described based on preferred embodiments.
First, raw oyster meat 1 is stored in the extraction container 2. Then, the aqueous solution 3 is stored in the extraction container 2. In addition, the solution used in the case of extraction is not limited to this aqueous solution 3. An ethanol solution may be used, or a solution using an acid or an alkali may be used.
For example, the stripped raw oyster meat 1 is carefully stored in the kama-type extraction container 2 having a so-called hemispherical shape without being crushed.

Next, the extraction container 2 is sealed, and the selection of whether the inside of the extraction container 2 is made normal pressure, pressurized or depressurized is made.
Then, the characteristics of atmospheric pressure extraction, the characteristics of pressure extraction, or the characteristics of decompression extraction are taken into consideration, and the most preferable or most desired extraction method is selected at the time of the extraction. Note that normal pressure extraction, which is a normal type, does not require special equipment, and is characterized by low cost.

Next, the difference in principle between the pressure extraction method and the pressure extraction method will be described.
Pressurized extraction is performed, for example, by a phenomenon in which components in oyster meat cells are eluted so that they move through the cell membrane and jump out of the cell by actively moving around under pressure. Therefore, pressurized extraction is excellent for extracting components existing deeply in the oyster meat cell, for example.

On the other hand, in the vacuum extraction, for example, the pressure outside the oyster meat cell is lower than the pressure inside the oyster meat cell, so that the component inside the oyster meat cell is extracted and eluted so as to be sucked out of the cell.
Therefore, it can be said that the reduced pressure extraction is excellent particularly when extracting components existing near the surface of the oyster meat cell.

  And in pressure extraction, there exists a merit which can extract many oyster meat extracts in a short time.

On the other hand, the reduced pressure extraction uses, for example, a state where the boiling point is low in a reduced pressure state and the temperature does not increase relatively.
That is, this is an effective extraction method especially when collecting oyster meat extract present on the cell surface.

  In addition, for example, oyster meat data such as the state of raw oyster meat (whether it is thick oyster meat, etc.) or the weight of raw oyster meat is taken into account, and when extracting oyster meat extract as a whole, pressurized extraction is preferable Or whether decompression extraction is preferable is selected and determined.

Here, a case where pressurized extraction is selected in the extraction selection step will be described.
First, the extraction container 2 is sealed and pressurized.
The degree of pressurization is not limited in any way, but the pressurization is performed at 1 atm or higher, and for example, about 1.1 to 2.5 atm is preferable. Further, the pressurizing time is not limited, but about 5 to 60 minutes is preferable. Further, the temperature at this time is 75 ° C. to 120 ° C. When the pressure is applied, the temperature rises from the normal pressure.

In the pressure extraction, it is not necessary to stir in the extraction container 2 so as not to damage the raw raw oyster meat 1. This is because according to the present invention, extraction can be performed efficiently without stirring. In addition, stirring may damage the active ingredient to be extracted.
Thus, various active ingredients are efficiently extracted from raw oyster meat 1. The various active ingredients are efficiently dissolved in the aqueous solution 3 in the extraction container 2.
Compared to the case of extraction under normal atmospheric pressure, this extraction amount has been confirmed to increase considerably. In addition, the same amount can be extracted with a significantly shortened extraction time compared to the conventional case.

  In addition, it is preferable to immerse and extract raw oyster meat 1 in an ethanol solution 4 instead of the aqueous solution 3. As a result of experiments, it has been confirmed that adenosine dissolves well in ethanol solution.

Next, the raw oyster meat 1 once used for the extraction can be reused.
First, the oyster meat extract-containing solution extracted by the first extraction is taken out from the extraction container 2 and only the raw oyster meat 1 is left. Next, the ethanol solution 4 is placed in the extraction container 2. The raw oyster meat 1 is delicate, and it is not preferable to take it out or take it in. Therefore, it is preferable to exchange the solution in the extraction container 2.

Therefore, the same extraction container 2 is usually used. However, when the first extraction is the decompression method and the second extraction is the pressurization method, the extraction container 2 itself must be replaced by changing from the decompression kettle to the pressurization kettle. However, there is no need for replacement in the case of a pot capable of both pressure reduction and pressurization, that is, a pressure reduction pressurization pot.
Here, for the second extraction, the second extraction container 5 is used. First, the second extraction container 5 is sealed and pressurized.

  Although the degree of this pressurization is not limited at all, it is 1 atm or more, and preferably about 1.1 to 2.5 atm. Further, the pressurizing time is not limited, but is understood to be about 5 to 60 minutes.

  Therefore, even the raw oyster meat 1 from which the oyster meat extract has been extracted once, the oyster meat extract containing adenosine is efficiently extracted. That is, as described above, it is confirmed that the oyster meat extract containing adenosine efficiently dissolves into the ethanol solution 4 in the second extraction container 5.

Next, the adenosine-containing oyster meat extract solution extracted from the raw oyster meat 1 by the first extraction and the ethanol solution from which the adenosine-containing oyster meat extract was extracted again by the second extraction of ethanol as described above were mixed. Thus, the mixed liquid 6 is generated.
And the liquid mixture 6 is concentrated. Here, the concentration method of the mixed solution 6 is not limited at all, and it is sufficient that the extracted oyster meat extract can be concentrated so that the solid content is, for example, about 15 to 45 (W / W)%.

  During this concentration, the temperature is usually increased and the work is performed at a high temperature in the range of 60 ° C. to 70 ° C. or higher. I can work.

Already, it has been found that concentration under reduced pressure at low temperature, that is, concentration at a low temperature, is a very important factor for the extraction of oyster meat extract.
That is, it has been found that when a protein solution, which is an extract of oyster meat, is maintained at a high temperature, the protein is condensed hard. However, protein condensation is slow at low temperatures. When the loose protein is in the solution, the area in contact with the solution increases.

  Thus, as will be described in the description of the next precipitation step, the protein is denatured and precipitated by contacting the ethanol solution 4. At this time, the slowly condensed protein has a larger area where the ethanol solution 4 and the protein are in contact with each other than the hard condensed protein, so that the protein is easily denatured and easily precipitated.

  However, adenosine does not precipitate and has the property of being easily dissolved in the ethanol solution 4. Therefore, adenosine is contained in a large amount in a so-called ethanol supernatant which does not spontaneously precipitate, for example, after the stirring and separation steps described below.

  By the way, what kind of concentration work is performed is not limited in the present invention, and is not limited to the above-described reduced-pressure low-temperature heating concentration, for example, concentration by atmospheric pressure heating, concentration by membrane, concentration by pressure heating, etc. It does not matter.

Next, the ethanol solution 4 is added to the concentrated solution, and the first stirring and separation described above is performed.
The stirring and separating step is not limited at all. Ordinary stirring operation may be performed, and then the natural precipitation of the precipitate may be waited and separated into the precipitate and the supernatant. Then, the precipitate is dried to produce a conventional dried oyster meat extract.
In the first stirring / separation step, as can be understood from FIG. 2, stirring / separation may be performed using the continuous centrifugal separator 7.

  Here, as shown in FIG. 5, the continuous centrifugal separator 7 includes a storage body 9 having a storage space portion 8 inside, and a storage body 9 disposed in the storage body 9. A rotating body 11 having a plurality of rotating blades 10 configured to be rotatable as directions; a rotating device 12 that rotates the rotating body 11; and an injection unit 13 that continuously injects a solution to be separated into the storage space 8; The discharge part 15 which centrifuges the solution injected from the injection part 13 and continuously discharges the fluid 14 (conventional sediment) separated from the side part of the storage space part 8, and the fluid 14 And a solution discharge portion 17 for continuously discharging the remaining solution 16 (conventional supernatant that does not precipitate) separated from the solution.

  Here, the rotating device 12 has a rotating shaft 18 projecting upward from substantially the center of the bottom of the continuous centrifugal separator 7 and a longitudinal direction (vertical direction in the present embodiment) of the rotating shaft 18 as a rotating direction. A rotating member (not shown) such as a rotating motor is provided.

  The rotating body 11 is fixed to the distal end of the rotating shaft 18, and the rotating body 11 is configured to rotate in the same manner as the rotating shaft 18. As can be understood from the drawing, the rotating body 11 has a cylindrical rotating shaft portion 19 having a hollow portion therein, and the outer periphery of the rotating shaft portion 19 projects outward in a skirt shape. Rotating feathers 10 are attached at predetermined equal intervals in the vertical direction.

  A distal end of a pipe-shaped injection tube 20 provided from the upper side is inserted into the rotary shaft portion 19 from the top, and between the injection tube 20 and the rotary shaft portion 19, a rotation is provided. The shaft portion 19 is configured to be rotatable while the space between the injection tube 20 and the rotation shaft portion 19 is sealed.

Thus, a solution obtained by adding the ethanol solution 4 to the concentrated solution of the oyster meat extract to be centrifuged is continuously sent from the delivery pipe 21 to the injection pipe 20 on the proximal end side of the injection pipe 20.
The delivered solution passes through an introduction path 22 formed inside the rotary shaft portion 19 and is guided into the storage body 9 from below the introduction path 22.

  When the solution is stored in the storage body 9, the rotating body 11 rotates at a high speed with the vertical direction as a rotation axis. As described above, the rotating body 11 is provided with a plurality of rotating blades 10... While the plurality of rotating blades 10 are rotated, the solution in the storage body 9 is stirred and centrifuged. Separation work is performed.

Usually, after stirring, the precipitate naturally settles for a predetermined long time, and after the natural precipitation is completed, the precipitate is separated into an upper supernatant and a precipitate, and the precipitate is taken out. In the continuous centrifugal separator 7, the conventional sediment to be precipitated is brought closer to the side wall of the container 9 by rotation and continuously discharged to the outside through the discharge holes 23 provided in the side wall. It has become. That is, it does not rely on natural precipitation as in the prior art, but is forced to separate from the supernatant.

  A plurality of the discharge holes 23 are provided at predetermined intervals in the circumferential direction of the side wall of the storage body 9.

  By the way, the discharge hole 23 is not opened from the beginning. In the vicinity of the discharge hole 23, the fluid 14 that becomes a conventional precipitate is collected by a centrifugal operation, and is configured to open and close the discharge hole 23 by, for example, operating the hydraulic fluid 24. The fluid 14 is configured to be discharged to the outside when the opening is opened.

Next, in the present invention, the remaining solution 16 is collected as a supernatant after spontaneous precipitation, that is, separated from the fluid 14 when the continuous centrifugal separator 7 is used.
After collection, moisture and the like are removed and concentrated until the remaining solution 16 becomes a paste. This concentration method is not limited at all. The concentration is not limited to low-pressure and low-temperature heating, and may be performed by, for example, normal pressure heating, membrane concentration, pressure heating concentration, or the like.

When it is concentrated to a paste, a paste-like or cocoon-like concentrated oyster meat extract is produced.
Even in this state, the active ingredient of the oyster meat extract is obtained and can be commercialized as a paste-like concentrated oyster meat extract.
As usage, it is shipped in bottling, and the customer scoops the concentrated oyster meat extract with a spoon, for example, dissolves it in tea or drinks it, or puts it in miso soup to make it a hidden taste. Has been proposed.

However, in the present invention, this concentrated paste-like concentrated oyster meat extract is placed in, for example, a 20% to 90% ethanol solution.
That is, the concentrated oyster meat extract is placed in a 20% to 90% ethanol solution, and stirring and separation are performed there.
The stirring and separation may be conventional stirring and separation, but it is preferable to use the continuous centrifugal separator 7 described above.

As described above, the solution obtained by adding the ethanol solution 4 to the concentrated solution of the oyster meat extract to be centrifuged is continuously sent from the delivery pipe 21 to the injection pipe 20 on the proximal end side of the injection pipe 20.
The delivered solution passes through an introduction path 22 formed inside the rotary shaft portion 19 and is guided into the storage body 9 from below the introduction path 22.

  When the solution is stored in the storage body 9, the rotating body 11 rotates at a high speed with the vertical direction as a rotation axis. As described above, the rotating body 11 is provided with a plurality of rotating blades 10... While the plurality of rotating blades 10 are rotating, the solution in the storage body 9 is stirred and centrifuged. Separation work is performed.

  And usually, after stirring, after waiting for a predetermined long time for the precipitate to spontaneously settle, and after the natural precipitation is finished, it is separated into the upper supernatant liquid and the precipitate, and the precipitate is taken out. In the continuous centrifugal separator 7, the conventional sediment to be precipitated is brought close to the side wall of the container 9 by rotation and continuously discharged to the outside through the discharge holes 23 provided in the side wall. It is like that. That is, it does not rely on natural precipitation as in the prior art, but is forced to separate from the supernatant.

A plurality of the discharge holes 23 are provided at predetermined intervals in the circumferential direction of the side wall of the storage body 9.
By the way, the discharge hole 23 has not been opened from the beginning, and in the vicinity of the discharge hole 23, the fluid 14 that becomes a conventional precipitate is collected by a centrifugal separation operation. The discharge hole 23 is configured to open and close, and the fluid 14 can be discharged to the outside when the discharge hole 23 is opened.
Then, using the continuous centrifugal separator 7 as described above, the remaining solution 16 separated from the fluid 14 is collected.

Thus, it was confirmed that a large amount of adenosine was contained in the remaining solution 16.
The amount of adenosine contained in the remaining solution 16 was recorded as 160 milligrams in 100 grams, that is, a high concentration of 0.16%.
Furthermore, the amount of adenosine contained in the residual solution 16 when stirring or centrifuging in the 70% ethanol solution 4 was 230 mg / 100, that is, 0.23%. The density was recorded.

BRIEF DESCRIPTION OF THE DRAWINGS It is structure explanatory drawing (the 1) explaining the structure of this invention. FIG. 3 is a configuration explanatory diagram (part 2) illustrating the configuration of the present invention. FIG. 3 is a configuration explanatory diagram (part 3) illustrating the configuration of the present invention; FIG. 6 is a configuration explanatory diagram (part 4) illustrating the configuration of the present invention; It is a structure explanatory drawing explaining the structure of a continuous centrifuge.

DESCRIPTION OF SYMBOLS 1 Raw oyster meat 2 Extraction container 3 Aqueous solution 4 Ethanol solution 5 2nd extraction container 6 Mixture 7 Centrifugal device 8 Storage space part 9 Storage body 10 Rotary blade 11 Rotating body 12 Rotating apparatus 13 Injection part 14 Fluid 15 Discharge part 16 Residual solution 17 Solution discharge part 18 Rotating shaft 19 Residual solution 20 Injection pipe 21 Delivery pipe 22 Introduction building 23 Discharge hole 24 Hydraulic fluid

Claims (9)

Raw oyster meat is stored in an extraction container, an ethanol solution is stored in the extraction container, and the oyster meat extract is extracted with ethanol.
Concentrate the extracted oyster meat extract,
Add concentrated oyster meat extract in ethanol solution and stir, separate into natural precipitate and supernatant,
Taking out the natural precipitate and the supernatant obtained after the stirring and separation,
Remove and concentrate the removed supernatant until it becomes paste-like or bowl-like,
Add the paste-like or bowl-like concentrate again into the ethanol solution, then stir and separate, dissolve the adenosine present in the oyster meat extract concentrate in the ethanol solution in the supernatant after separation, and dissolve the solution The ethanol solution was removed from the liquid and the oyster meat extract with adenosine was taken out.
A method for producing an adenosine-containing oyster meat extract.
Raw oyster meat is stored in an extraction container, an ethanol solution is stored in the extraction container, and the oyster meat extract is extracted with ethanol.
Concentrate the extracted oyster meat extract,
Add concentrated oyster meat extract in ethanol solution and stir, separate into natural precipitate and supernatant,
Taking out the natural precipitate and the supernatant obtained after the stirring and separation,
Remove and concentrate the removed supernatant until it becomes paste-like or bowl-like,
Add the paste-like or bowl-like concentrated solution again into the ethanol solution to make an ethanol solution with a concentration of 20% to 90%, then stir and separate, and the oyster meat extract in the ethanol solution in the separated supernatant. Adenosine present in the concentrate was dissolved, the ethanol solution was removed from the solution, and the oyster meat extract containing adenosine was taken out.
A method for producing an adenosine-containing oyster meat extract.
Raw oyster meat is stored in an extraction container, an ethanol solution is stored in the extraction container, and the oyster meat extract is extracted with ethanol.
Concentrate the extracted oyster meat extract,
Add concentrated oyster meat extract in ethanol solution and stir, separate into natural precipitate and supernatant,
Taking out the natural precipitate and the supernatant obtained after the stirring and separation,
Remove and concentrate the removed supernatant until it becomes paste-like or bowl-like,
Add the paste-like or bowl-like concentrate again into the ethanol solution to make a 70% ethanol solution, then stir and separate, and separate the supernatant into the ethanol solution into the oyster meat extract concentrate. The existing adenosine was dissolved, the ethanol solution was removed from the solution, and the oyster meat extract containing adenosine was taken out.
A method for producing an adenosine-containing oyster meat extract.
Of the stirring, at least the last stirring is performed by a continuous centrifugal separator.
The method for producing an adenosine-containing oyster meat extract according to claim 1, 2, or 3.
The pressure at the time of extraction of the oyster meat extract is performed by selecting either reduced pressure or increased pressure, or normal pressure,
The method for producing an adenosine-containing oyster meat extract according to claim 1, claim 2, claim 3, or claim 4.
Extraction of the oyster meat extract was performed a second time from the oyster meat from which the extract was extracted once, and the first extract and the second extract were mixed to form an extract.
The method for producing an adenosine-containing oyster meat extract according to claim 1, 2, 3, 4, or 5.
Concentration of the extracted oyster meat extract was concentrated by heating under reduced pressure and low temperature,
A method for producing an adenosine-containing oyster meat extract according to claim 1, claim 2, claim 3, claim 4, claim 5 or claim 6.
Concentration of the removed supernatant was concentrated by heating under reduced pressure and low temperature.
The method for producing an adenosine-containing oyster meat extract according to claim 1, claim 2, claim 3, claim 4, claim 5, claim 6, or claim 7.
The raw oyster meat is stripped,
A method for producing an adenosine-containing oyster meat extract according to claim 1, claim 2, claim 3, claim 4, claim 5, claim 6, claim 7 or claim 8.

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