JP2012126720A - コラーゲンの産生促進剤、プロテオグリカン産出促進剤及び軟骨細胞の遊走促進剤 - Google Patents
コラーゲンの産生促進剤、プロテオグリカン産出促進剤及び軟骨細胞の遊走促進剤 Download PDFInfo
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| Publication number | Priority date | Publication date | Assignee | Title |
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| JP2014062048A (ja) * | 2012-09-19 | 2014-04-10 | Sanyo Chem Ind Ltd | コラーゲン産生促進剤、コラーゲン産生促進用培養液、抗老化剤、コラーゲン産生促進用ゲル、及び、コラーゲン産生用ゲルの製造方法 |
| JP2017525675A (ja) * | 2014-07-09 | 2017-09-07 | メイヨ・ファウンデーション・フォー・メディカル・エデュケーション・アンド・リサーチ | 回旋筋腱板異常の処置 |
| US10596192B2 (en) | 2015-07-16 | 2020-03-24 | Mayo Foundation For Medical Education And Research | Treating rotator cuff conditions |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2005289819A (ja) * | 2004-03-31 | 2005-10-20 | Nippon Meat Packers Inc | 皮膚組織の再生及び肌質改善効果のあるペプチド、並びに該ペプチドを含有する食品、医薬品、又は外用剤 |
| JP2007001981A (ja) * | 2006-07-10 | 2007-01-11 | Nippon Meat Packers Inc | 皮膚組織の再生及び肌質改善効果のあるペプチドを含有する化粧品 |
| JP2010024200A (ja) * | 2008-07-23 | 2010-02-04 | Dhc Co | 生体コラーゲン合成促進剤並びに生体コラーゲン合成促進用飲食品、化粧品及び医薬部外品 |
| WO2010125722A1 (ja) * | 2009-05-01 | 2010-11-04 | 国立大学法人 千葉大学 | 人工コラーゲンを用いた軟骨培養用基材及び該基材を用いた軟骨再生治療方法 |
Family Cites Families (1)
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| JP2009067727A (ja) * | 2007-09-13 | 2009-04-02 | Nara Institute Of Science & Technology | コラーゲン様ポリペプチドおよびそれを含有する皮膚外用組成物 |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2005289819A (ja) * | 2004-03-31 | 2005-10-20 | Nippon Meat Packers Inc | 皮膚組織の再生及び肌質改善効果のあるペプチド、並びに該ペプチドを含有する食品、医薬品、又は外用剤 |
| JP2007001981A (ja) * | 2006-07-10 | 2007-01-11 | Nippon Meat Packers Inc | 皮膚組織の再生及び肌質改善効果のあるペプチドを含有する化粧品 |
| JP2010024200A (ja) * | 2008-07-23 | 2010-02-04 | Dhc Co | 生体コラーゲン合成促進剤並びに生体コラーゲン合成促進用飲食品、化粧品及び医薬部外品 |
| WO2010125722A1 (ja) * | 2009-05-01 | 2010-11-04 | 国立大学法人 千葉大学 | 人工コラーゲンを用いた軟骨培養用基材及び該基材を用いた軟骨再生治療方法 |
Non-Patent Citations (1)
| Title |
|---|
| JPN6015039573; 北島 勲: 'Ｉ型プロコラーゲンＣ末端プロペプチド（ＰＩＣＰ）、Ｉ型コラーゲンＣ末端テロペプチド（ＩＣＴＰ）' 日本臨床（増刊号）広範囲 血液・尿化学検査，免疫学的検査（第７版）２-その数値をどう読むか- 第６８巻，増刊号１, 201001, ｐ．２５２-２５５ * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2014062048A (ja) * | 2012-09-19 | 2014-04-10 | Sanyo Chem Ind Ltd | コラーゲン産生促進剤、コラーゲン産生促進用培養液、抗老化剤、コラーゲン産生促進用ゲル、及び、コラーゲン産生用ゲルの製造方法 |
| JP2017525675A (ja) * | 2014-07-09 | 2017-09-07 | メイヨ・ファウンデーション・フォー・メディカル・エデュケーション・アンド・リサーチ | 回旋筋腱板異常の処置 |
| US10610570B2 (en) | 2014-07-09 | 2020-04-07 | Mayo Foundation For Medical Education And Research | Treating rotator cuff conditions |
| US10596192B2 (en) | 2015-07-16 | 2020-03-24 | Mayo Foundation For Medical Education And Research | Treating rotator cuff conditions |
| US11376280B2 (en) | 2015-07-16 | 2022-07-05 | Mayo Foundation For Medical Education And Research | Treating rotator cuff conditions |
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| JP2017039698A (ja) | 2017-02-23 |
| JP6260913B2 (ja) | 2018-01-17 |
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