JP2011510654A - 有用な抗体を発現する雑種細胞の作成方法 - Google Patents

有用な抗体を発現する雑種細胞の作成方法 Download PDF

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JP2011510654A
JP2011510654A JP2010545017A JP2010545017A JP2011510654A JP 2011510654 A JP2011510654 A JP 2011510654A JP 2010545017 A JP2010545017 A JP 2010545017A JP 2010545017 A JP2010545017 A JP 2010545017A JP 2011510654 A JP2011510654 A JP 2011510654A
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antibody
cells
nucleic acid
hybridoma
antibodies
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JP2011510654A5 (https=
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スコット・ケイ・デサイン
シャラド・ピー・アデカー
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Thomas Jefferson University
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Thomas Jefferson University
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/12—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from bacteria
    • C07K16/1267—Gram-positive bacteria
    • C07K16/1282—Clostridium (G)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04—Antibacterial agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P39/00—General protective or antinoxious agents
    • A61P39/02—Antidotes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C12N5/12—Fused cells, e.g. hybridomas
    • C12N5/16—Animal cells
    • C12N5/163—Animal cells one of the fusion partners being a B or a T lymphocyte
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/21—Immunoglobulins specific features characterized by taxonomic origin from primates, e.g. man
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/70—Immunoglobulins specific features characterized by effect upon binding to a cell or to an antigen
    • C07K2317/76—Antagonist effect on antigen, e.g. neutralization or inhibition of binding
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/90—Immunoglobulins specific features characterized by (pharmaco)kinetic aspects or by stability of the immunoglobulin
    • C07K2317/92—Affinity (KD), association rate (Ka), dissociation rate (Kd) or EC50 value
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/50—Cell markers; Cell surface determinants
    • C12N2501/52—CD40, CD40-ligand (CD154)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Immunology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Communicable Diseases (AREA)
  • Oncology (AREA)
  • Toxicology (AREA)
  • Neurology (AREA)
  • Neurosurgery (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
JP2010545017A 2008-01-28 2009-01-28 有用な抗体を発現する雑種細胞の作成方法 Pending JP2011510654A (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US6258408P 2008-01-28 2008-01-28
PCT/US2009/000562 WO2009105150A2 (en) 2008-01-28 2009-01-28 Method of making hybrid cells that express useful antibodies

Related Child Applications (1)

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JP2015009809A Division JP5923186B2 (ja) 2008-01-28 2015-01-21 有用な抗体を発現する雑種細胞の作成方法

Publications (2)

Publication Number Publication Date
JP2011510654A true JP2011510654A (ja) 2011-04-07
JP2011510654A5 JP2011510654A5 (https=) 2012-03-15

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JP2010545017A Pending JP2011510654A (ja) 2008-01-28 2009-01-28 有用な抗体を発現する雑種細胞の作成方法
JP2015009809A Active JP5923186B2 (ja) 2008-01-28 2015-01-21 有用な抗体を発現する雑種細胞の作成方法

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US (1) US8999707B2 (https=)
EP (1) EP2242836B1 (https=)
JP (2) JP2011510654A (https=)
WO (1) WO2009105150A2 (https=)

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* Cited by examiner, † Cited by third party
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US7563874B2 (en) 1998-08-31 2009-07-21 The Regents Of The University Of California Therapeutic monoclonal antibodies that neutralize botulinum neurotoxins
CA2680741A1 (en) 2007-03-22 2009-01-15 The Regents Of The University Of California Therapeutic monoclonal antibodies that neutralize botulinum neurotoxins
WO2009131605A2 (en) * 2008-01-28 2009-10-29 Thomas Jefferson University Fusion partner cell line for preparation of hybrid cells expressing human antibodies
WO2010003529A1 (en) * 2008-06-16 2010-01-14 University Of Zurich Method of providing immunoglobulin secreting b lymphocytes and human antibodies
WO2010014854A2 (en) 2008-07-31 2010-02-04 The Regents Of The University Of California Antibodies that neutralize botulinum neurotoxins
WO2011028961A2 (en) 2009-09-04 2011-03-10 Xoma Technology Ltd. Anti-botulism antibody coformulations
WO2011028962A1 (en) * 2009-09-04 2011-03-10 Xoma Technology Ltd. Antibody coformulations
PL2400298T3 (pl) * 2010-05-28 2014-01-31 Hoffmann La Roche Sposób hodowania pojedynczych komórek b oraz wytwarzania swoistych przeciwciał
US9243057B2 (en) 2010-08-31 2016-01-26 The Regents Of The University Of California Antibodies for botulinum neurotoxins
EP2646466B1 (en) * 2010-12-02 2017-03-29 AIMM Therapeutics B.V. Means and methods for producing high affinity antibodies
WO2013000982A1 (en) 2011-06-27 2013-01-03 Vivalis Method for screening cells
CN103946236B (zh) * 2011-11-23 2018-02-16 弗·哈夫曼-拉罗切有限公司 表达cd40l的哺乳动物细胞及其用途
US10017739B2 (en) 2012-09-06 2018-07-10 Duke University Methods of expanding and assessing B cells and using expanded B cells to treat disease
WO2014138733A2 (en) * 2013-03-08 2014-09-12 Dessain Scott K Compositions and methods for making human antibodies
US20160014600A1 (en) * 2014-07-10 2016-01-14 Bank Of America Corporation Identification of Potential Improper Transaction
US20160253708A1 (en) * 2015-02-26 2016-09-01 Dropbox, Inc. Method and system for efficiently serving upsell content based on complex user archetypes
ES2970828T3 (es) 2016-02-10 2024-05-30 Univ Rutgers Nuevos anticuerpos anti-lam
WO2018112407A1 (en) 2016-12-15 2018-06-21 Duke University Antibodies and methods for depleting regulatory b10 cells and use in combination with immune checkpoint inhibitors
KR20210090172A (ko) 2018-10-02 2021-07-19 이뮤놈 인코포레이티드 Epn1을 표적화하는 항체
WO2022150809A1 (en) 2021-01-05 2022-07-14 Immunome, Inc. Antibody cocktail against sars-cov-2 spike protein
CN114350606A (zh) * 2022-01-05 2022-04-15 上海药明生物医药有限公司 一种富集大鼠浆细胞及建立浆细胞杂交瘤的方法

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WO2007063415A2 (en) * 2005-08-23 2007-06-07 Iq Corporation Methods of producing stable b-lymphocytes

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JP3150991B2 (ja) * 1991-04-10 2001-03-26 協和醗酵工業株式会社 ハイブリドーマの製造法
US6541225B1 (en) 2000-01-26 2003-04-01 Raven Biotechnologies, Inc. Methods and compositions for generating human monoclonal antibodies
AU2002357932A1 (en) 2001-12-18 2003-06-30 Whitehead Institute For Biomedical Research Fusion partner cells and uses thereof
GB2422845B (en) 2003-11-19 2007-08-01 Us Gov Health & Human Serv Method of inducing memory B cell development and terminal differentiation
WO2009131605A2 (en) * 2008-01-28 2009-10-29 Thomas Jefferson University Fusion partner cell line for preparation of hybrid cells expressing human antibodies

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007063415A2 (en) * 2005-08-23 2007-06-07 Iq Corporation Methods of producing stable b-lymphocytes

Non-Patent Citations (4)

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Title
JPN6013058515; Ｊ　Ｉｍｍｕｎｏｌ． Ｖｏｌ．１６９，　Ｎｏ．８, 20021015, ｐ．４２９８-４３０６ *
JPN6013058518; Ｊ　Ｉｍｍｕｎｏｌ　Ｍｅｔｈｏｄｓ． Ｖｏｌ．２９１，　Ｎｏ．１-２, 200408, ｐ．１０９-１２２ *
JPN6013058519; ＤＥＳＳＡＩＮ，　Ｓ．Ｋ．　ｅｔ　ａｌ．: 'ＳＥＳＳＩＯＮ　７：　ＩＮＦＥＣＴＩＯＵＳ　ＤＩＳＥＡＳＥＳ　Ｉ，　Ｕｓｅ　ｏｆ　ａ　ｎｏｖｅｌ　ｈｅｔｅｒｏ-ｈｙｂｒｉｄｏｍａ　ｍｅｔｈｏｄ　ｔｏ　ｃｌｏｎｅ　ｐｏｔｅｎｔ，　ｈｉｇｈ　ａｆｆｉｎ' ＨＡＨ　２００７　Ｓｅｃｏｎｄ　Ｃｉｒｃｕｌａｒ　ａｎｄ　Ｐｒｏｖｉｓｉｏｎａｌ　Ｃｏｎｆｅｒｅｎｃｅ　Ｐｒｏｇｒａｍ，　［ｏｎｌｉｎｅ］ , 200710 *
JPN6013058521; ＤＥＳＳＡＩＮ，　Ｓ．Ｋ．　ｅｔ　ａｌ．: 'Ｓｅｓｓｉｏｎ　７：　Ｉｎｆｅｃｔｉｏｕｓ　ｄｉｓｅａｓｅｓ　Ｉ，　Ｕｓｅ　ｏｆ　ａ　ｎｏｖｅｌ　ｈｅｔｅｒｏ-ｈｙｂｒｉｄｏｍａ　ｍｅｔｈｏｄ　ｔｏ　ｃｌｏｎｅ　ｐｏｔｅｎｔ，　ｈｉｇｈ　ａｆｆｉｎ' Ｈｕｍａｎ　Ａｎｔｉｂｏｄｉｅｓ Ｖｏｌ．１６，　Ｎｏ．１-２, 2007, ｐ．２８-２９ *

Also Published As

Publication number Publication date
WO2009105150A3 (en) 2010-03-18
EP2242836A4 (en) 2011-05-25
EP2242836A2 (en) 2010-10-27
WO2009105150A2 (en) 2009-08-27
JP2015083016A (ja) 2015-04-30
US8999707B2 (en) 2015-04-07
EP2242836B1 (en) 2015-05-20
US20110002937A1 (en) 2011-01-06
JP5923186B2 (ja) 2016-05-24

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