JP2011195523A - Elastase activity inhibitor - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a novel elastase activity inhibitor having good safety.SOLUTION: The elastase activity inhibitor includes a Sesamum indicum sprout extract as an active ingredient.

Description

  The present invention relates to a novel elastase activity inhibitor.

  Elastin fiber is a type of connective tissue fiber in the extracellular matrix that is distributed in organs and tissues throughout the body, including blood vessels, ligaments, lungs, and skin in the body of mammals and fish such as humans. It is known that the α-helix structure is formed by cross-linking and plays an important role in maintaining the flexibility of the tissue. On the other hand, the skin consists of the epidermis, dermis, and subcutaneous tissue, the epidermis protects the body from external dryness and foreign matter, the subcutaneous tissue softens external impacts by subcutaneous fat, etc., and the dermis is fibroblasts and the outside of these cells. It is constituted by the dermal extracellular matrix that supports the skin structure and plays an important role in maintaining the structure of the skin. In the skin, elastin fibers run parallel to the epidermis surface at the bottom of the papillary layer, the middle fibers of the papillary layer (elaunin fiber), and then the thin terminal fibers come out and go vertically to the basement membrane of the epidermis It is said that there is a thing (oxytalan fiber).

  The phenomenon called skin aging such as skin wrinkle formation, dullness, loss of texture, and loss of elasticity is attributed to a decrease in the production ability of elastin fibers and the like due to a decrease in the proliferative ability of fibroblasts due to aging. However, external irritation such as exposure of the skin to ultraviolet rays, significant drying of the air, excessive skin washing, etc., causes degradation of the elastin fibers, resulting in wrinkle formation, dullness, loss of texture, loss of elasticity, etc. It is said that symptoms such as rough skin and aging of the skin appear.

  It is considered that elastin fibers are degraded by elastase. Elastase is an enzyme belonging to the serine protease family. For example, pancreatic elastase is a marker for various pancreatic diseases because of increased blood levels due to pancreatic lesions. Elastase derived from leukocytes is an early stage that cannot be confirmed with the naked eye. It is known that the blood concentration also rises in the microinflammation. In skin, for example, it is speculated that there is accumulation of micro-inflammation when irradiated with ultraviolet A waves, and it has been confirmed that elastase activity increases (for example, see Patent Document 1).

For this reason, it is said that suppressing the action of elastase in the dermis and preventing the degradation of elastin fibers is effective for preventing skin aging such as wrinkles and tarmi. For example, Brucella rancifolia extract (for example, Patent Document 2) See), sweet pea extract (for example, see Patent Document 3), Iceland moss extract (for example, see Patent Document 4), mannentake extract (for example, see Patent Document 5), plant / fungal extract, etc. Has been proposed as an elastase activity inhibitor.

  Further, for example, glucosamine, glucosamine derivatives or salts thereof (for example, see Patent Document 6), phosphonic acid derivatives, mercaptopropionamide derivatives (see, for example, Patent Document 7), corosolic acid (for example, see Patent Document 8). And elastase activity inhibitors containing compounds such as 1,6-heptadiene-3,5-dione derivatives (see, for example, Patent Document 9) as active ingredients.

  On the other hand, elastase activity in the skin tissue corresponds to the hair cycle, and it has been reported that an increase in the elastase activity in the skin tissue is essential for hair follicle formation and growth (see, for example, Patent Document 10). A hair growth inhibitor containing an activity inhibitor as an active ingredient has been proposed (see, for example, Patent Document 11).

Sesame is an annual plant of Pedaliaceae Sesamum, and the cultivated species is Sesamuun indicum L. Regarding the use in the cosmetics field of components obtained from germinated sesame seeds (sprouts), immunostimulatory effect by activating macrophages (for example, see Patent Document 12), hydroxy radical scavenging activity effect (for example, Patent Document) 13) and the like, and various effects of the sesame seed germination extract are attracting attention.
However, it is not known at all about application as an elastase activity inhibitor in the effectiveness of the extract of sesame seeds.

Japanese Patent No. 3749769 JP 2002-255734 A JP 2006-282617 A JP 2007-302607 A JP-A-2005-23021 JP 2004-83432 A JP 2006-104209 A JP 2006-265232 A JP 2006-347897 A JP-A-11-199450 JP 2005-343887 A JP 2006-298773 A Japanese Patent Laid-Open No. 08-231953

  Some of the conventional elastase activity inhibitors described above have not been confirmed to be safe, stable, etc., and the elastase activity inhibitory activity effect may not be fully confirmed. Therefore, the subject of this invention is providing the elastase activity inhibitor which uses the compound with the high effect of suppressing elastase activity as an active ingredient.

  As a result of intensive studies on various plant extracts in order to solve the above-mentioned problems, the present inventors have found that an extract from sesame seeds shows an elastase activity inhibitory effect and completed the present invention.

  According to the present invention, a novel elastase activity inhibitor with good safety can be provided.

The figure which shows the elastase activity inhibitory effect evaluation test and its result.

  Hereinafter, the present invention will be described in detail. In the present specification, “to” means a range including numerical values before and after.

Sesame germination (Sesamum) used in the present invention
indicum Lenne) is a plant belonging to the sesame family Sesame genus and can be used regardless of the type and production area of white sesame, black sesame, etc., as long as it has not been subjected to high temperature treatment such as brewing broth. This is evenly spread on a suitable medium capable of containing water or water, such as agar, quartz sand, sea sand, absorbent cotton, sand, earth, etc., preferably a sterilized medium, and 10-50 ° C., preferably 30-40 Culturing is performed for 5 to 100 hours, preferably 24 to 72 hours while supplementing water at a suitable time. If the length is shorter than this, germination is not sufficient, and if it is longer, leaves are not preferable. Culture may be performed under illumination or in dark conditions.

  This sesame germinant extract can be prepared by a general method. Although it does not specifically limit as an extraction solvent, For example, Water; Lower monohydric alcohols, such as methyl alcohol and ethyl alcohol; Liquid polyhydric alcohols, such as glycerol, propylene glycol, and 1, 3- butylene glycol; Ketones, such as acetone; Ethyl One or more of ethers such as ether; esters such as ethyl acetate; and the like can be used. Extraction can be performed by immersing the sesame germinant extract in a solvent at room temperature or under heating for a predetermined time. Moreover, pre-processing, such as drying, shredding, pressing or fermentation, can also be performed before extraction.

  The sesame germinant extract can be used directly as an elastase activity inhibitor after preparation. Further, if desired, it can be used as an elastase activity inhibitor after being left to mature for an appropriate period. If necessary, it can be used after being subjected to a purification treatment such as deodorization and decolorization by filtration or ion exchange resin within a range that does not affect the effect of the present invention. Further, a fraction having high activity can be taken out and used by using a separation means such as liquid chromatography.

  The sesame germinant extract may be in any form such as liquid, paste or gel. That is, the liquid extract containing the extraction solvent may be used as it is or after being concentrated, or may be used after the extract is dried and solidified by drying under reduced pressure or freeze drying. It can also be dried by spray drying or the like and used as a powder. Furthermore, these solid or powder extracts may be appropriately redissolved in a solvent and used as an extract.

  The elastase activity inhibitor of the present invention can be used for various applications. Since the elastase activity inhibitor of the present invention has high safety and excellent stability at the time of blending, it can be used as an active ingredient for blending into foods (including beverages) and cosmetics or skin external preparations. Is preferred.

A cosmetic or external preparation for skin containing the elastase activity inhibitor of the present invention suppresses the activity of elastase when applied to the skin. As a result, excessive degradation of elastin does not occur, skin flexibility and elasticity are maintained, and senile phenomena such as sagging and wrinkles are improved. That is, it has excellent effects as an anti-aging cosmetic or a skin external preparation, and a wrinkle / sag improvement cosmetic or a skin external preparation.
In addition, since elastase may be involved in immune cell migration in an inflammatory reaction, it is also useful as an anti-inflammatory cosmetic or external skin preparation containing the elastase activity inhibitor of the present invention.
Furthermore, since elastase may be involved in hair follicle formation, the cosmetic or skin external preparation containing the elastase activity inhibitor of the present invention is a cosmetic or skin external preparation that controls hair growth and hair growth. It is also useful.

  The blending amount of the elastase activity inhibitor of the present invention in the cosmetic or the external preparation for skin is not particularly limited, but generally, it is converted into solid content with respect to the total composition from the viewpoint of inhibiting elastase activity. The content is preferably 0.0001 to 1% by mass (hereinafter simply expressed as%), more preferably 0.001 to 0.1%. Within this range, the elastase activity inhibitor of the present invention can be stably blended, and a high anti-aging effect and sagging / wrinkle improving effect can be exhibited.

In the cosmetic or external preparation for skin, other active ingredients such as a whitening effect, an anti-aging effect, or an effect of suppressing wrinkle formation due to exposure to ultraviolet rays are within the range not impairing the effects of the present invention. Ingredients may be blended. More specifically, examples include, but are not limited to, UV protection agents, antibacterial agents, whitening agents, anti-inflammatory agents, cell activators, active oxygen scavengers, moisturizers and the like.

  Examples of the UV protection agent include benzophenone compounds, PABA compounds, cinnamic acid compounds, and salicylic acid compounds, and more specifically, 4-tert-butyl-4′-methoxydibenzoylmethane, p-methoxy. Examples include 2-ethylhexyl cinnamate, 2-hydroxy-4-methoxybenzophenone, 2-phenyl-benzimidazole-5-sodium sulfate, titanium oxide, and zinc oxide.

  Examples of the antibacterial agent include benzoic acid, sodium benzoate, p-hydroxybenzoate ester, benzalkonium chloride, phenoxyethanol, isopropylmethylphenol, and the like.

  The whitening agent has the effect of preventing the occurrence of darkening of the skin caused by sunburn or the like, stains caused by pigmentation, buckwheat, etc., such as arbutin, ellagic acid, linoleic acid, vitamin C and derivatives, vitamin E and Its derivatives, glycyrrhizic acid and its derivatives, glycyrrhetinic acid and its derivatives, tranexamic acid, placenta extract, chamomile extract, licorice extract, age extract, sea urchin extract, seaweed extract, cucumber extract, coconut extract, Gokahi extract, rice bran extract, wheat germ extract, saicin extract, hawthorn extract, sunpens extract, shirayuri extract, peonies extract, sempukuka extract, soybean extract, tea extract, molasses extract, beechlen Extract, Grape extract, Hop extract, Mikaika extract, Mokka extract, Yu Noshita extract, and the like.

  Anti-inflammatory agents have the effect of suppressing inflammation such as hot flashes and erythema on the skin after sunburn. For example, sulfur and its derivatives, glycyrrhizic acid and its derivatives, glycyrrhetinic acid and its derivatives, Altea extract, Ashitaba Extract, Arnica extract, Ginseng extract, Nettle extract, Oat extract, Hypericum extract, Chamomile extract, Snapdragon extract, Watercress extract, Comfrey extract, Salvia extract, Shikon extract, Perilla extract Product, birch extract, gentian extract and the like.

  The cell activator is used for the purpose of improving rough skin, such as caffeine, chicken crown extract, shell extract, royal jelly, silk protein and its degradation product or derivatives thereof, lactoferrin or its degradation product, chondroitin sulfate, Mucopolysaccharides such as hyaluronic acid or their salts, collagen, yeast extract, lactic acid bacterium extract, bifidobacteria extract, fermentation metabolic extract, ginkgo biloba extract, barley extract, sembly extract, beetle extract, carrot extract Products, rosemary extract, glycolic acid, citric acid, lactic acid, malic acid, tartaric acid, succinic acid and the like.

  The active oxygen scavenger has an action such as a lipid peroxide production inhibitor, such as superoxide dismutase, mannitol, quercetin, catechin and derivatives thereof, rutin and derivatives thereof, button pi extract, yashajitsu extract, melissa Extracts, Rahan fruit extract, retinol and its derivatives, vitamin A such as carotenoid, thiamine and its derivatives, riboflavin and its derivatives, pyridoxine and its derivatives, vitamin B such as nicotinic acid and its derivatives, tocopherol and its derivatives, etc. Vitamin E, dibutylhydroxytoluene and butylhydroxyanisole.

  The humectant includes, for example, proteins such as elastin and keratin or derivatives thereof, hydrolysis and salts thereof, amino acids such as glycine, serine, asoaraginic acid, glutamic acid, arginine and theanine and derivatives thereof, sorbitol, erythritol, trehalose, Inositol, glucose, sucrose and derivatives thereof, dextrin and derivatives thereof, saccharides such as honey, D-panthenol and derivatives thereof, urea, phospholipid, ceramide, auren extract, camphor extract, diau extract, senkyu extract, Examples of this include mallow mushroom extract, ginger extract, dokudami extract, hamamelis extract, bodaige extract, maronier extract, quince extract and the like.

  In addition, the cosmetic or skin external preparation may be used in the manufacture of cosmetics, quasi drugs, skin external preparations, and the like, such as water (purified water, Hot spring water, deep water, etc.), oil agent, surfactant, metal soap, gelling agent, powder, alcohol, water-soluble polymer, film-forming agent, resin, inclusion compound, antibacterial agent, fragrance, deodorant , Salts, pH adjusters, fresheners, extracts derived from plants, animals and microorganisms, active oxygen scavengers, blood circulation promoters, astringents, antiseborrheic agents, moisturizers, chelating agents, keratolytic agents, enzymes, hormones Other vitamins can be added as needed.

Hereinafter, the present invention will be described more specifically with reference to examples. However, the scope of the present invention is not limited to the following examples.
[Production Example 1: Preparation 1 of Sesame Germ Extract]
Pre-sterilized quartz sand was spread on a stainless steel vat, Chinese sesame seeds were sown on it, and cultured and germinated in a constant temperature bath at 40 ° C. while thoroughly sprayed with distilled water. The germination rate was 80% or more. 100 g of germinated whole plant parts having the same state were put into 1 L of 70% aqueous ethyl alcohol solution and extracted by heating under reflux. The insoluble material was filtered, and the filtrate was concentrated under reduced pressure to obtain a solid sesame germinant extract.
[Example 1: Elastase activity inhibition test]
As a test solution, a 0.1% solution was prepared as a solid content concentration of the sesame germinated extract. The sample used here was a mixed solution of ethanol: water = 1: 1 by volume ratio.
The elastase activity inhibition test was performed as follows using elastase enzyme solution derived from swine (manufactured by Sigma) and N-Succinyl-Ala-Ala-Ala-p-nitronelide as substrates.
The enzyme solution was adjusted to a concentration of 0.05 units / mL.
The substrate solution was prepared by dissolving the substrate in dimethyl sulfoxide (DMSO) to obtain a 0.1 mol / L concentration solution, and then diluting with 0.05 mol / L Tris-HCl (pH 8.8) buffer solution to obtain 1 mmol. / L concentration solution was prepared.
After mixing 100 μL of the substrate solution and 50 μL of the 1% sample solution, 50 μL of the enzyme solution was added and reacted at 37 ° C. for 30 minutes. Thereafter, the absorbance at a wavelength of 405 nm was measured with a spectrophotometer, the amount of produced nitroaniline was determined, and the elastase activity inhibition rate was calculated. The elastase activity inhibition rate was calculated by the following formula.

Elastase activity inhibition rate (%) = {1− (A−B) / (C−D)} × 100

A: Absorbance at a wavelength of 405 nm of the mixed solution when the enzyme solution is added after mixing the substrate solution and the sample solution B: Absorbance at a wavelength of 405 nm of the mixed solution when the substrate solution and the sample solution are mixed C: Sample Instead of the solution, after the solvent of the solution is mixed with the substrate solution in the above ratio, the absorbance at a wavelength of 405 nm of the mixture solution when the enzyme solution is added. D: The sample solution and the enzyme solution are not added. Absorbance at 405 nm

In addition, in order to verify the elastase activity inhibitory effect of the sesame seed germ extract of the present invention, it is known as a complete protease inhibitor cocktail manufactured by Roche and an elastase activity inhibitor (for example, JP-A-2003-2820). Marigold extract (CALENDULAOFFICINALIS. Asteraceae) was used. It has already been confirmed that the complete protease inhibitor cocktail used in the present invention is a reagent that always has a certain inhibitory ability for various proteases and is effective for the present elastase. A result is combined with the result of a sesame germination extract, and is shown collectively in FIG.
From the results shown in the graph of FIG. 1, it was found that the elastase activity inhibitor of the present invention has a remarkable elastase activity inhibitory effect equivalent to that of the complete protease inhibitor cocktail. Moreover, it turned out that it has a sufficiently high elastase activity inhibitory effect compared with a marigold extract. Therefore, by using the elastase activity inhibitor of the present invention, a higher elastase activity inhibitory action can be obtained. In addition, even when the blending amount is reduced from the conventional level, the same or higher effect can be obtained. Therefore, the range of selection of the blending composition is widened, and it is advantageous in terms of formulation to use the elastase activity inhibitor of the present invention.

The following are examples of extracts, cosmetics or external preparations for skin containing the elastase activity inhibitor of the present invention.
[Production Example 2: Preparation 2 of Sesame Germ Extract]
Sesame seeds (indian) cultivated for 2 days at 37 ° C and germinated were separated from the medium, and then pulverized with a homogenizer, 100 g was extracted at 40 ° C for 20 hours with 1 L of 50% dipropylene glycol solution. did. The precipitate was filtered by leaving it for 24 hours to obtain an extract. The solid content concentration of this extract was 2.0%.
[Production Example 3: Preparation 3 of Sesame Germ Extract]
200 g of germinated sesame seeds (produced in China) that were cultured and germinated at 40 ° C. for 2 days in the same manner as in Example 1 were extracted and filtered in 1 L of purified water for 72 hours in a cool and dark place. The filtrate was concentrated under reduced pressure until the volume was reduced to half, and 1,3-butylene glycol and purified water were added to adjust the concentration of 1,3-butylene glycol to 30%. The solid content concentration of this extract was 5.0%.
[Example 2: Face wash]
(Manufacturing method)
A. The following components (1) to (7) are dissolved by heating.
B. The following components (8) to (11) are dissolved by heating.
C. Add B to A and mix.
D. After cooling C, the following components (12) to (14) were added and mixed to obtain a face wash.

(Ingredient) (%)
(1) Lauric acid 5.0
(2) Myristic acid 18.5
(3) Stearic acid 6.0
(4) Glycerin 12.0
(5) Polyethylene glycol 1500 5.0
(6) Potassium hydroxide 6.5
(7) Purified water remaining amount (8) Palm oil fatty acid diethanolamide 5.0
(9) Palm oil fatty acid methyl taurine sodium 1.8
(10) Polyoxyethylene (7.5E.O.) lauryl ether 2.0
(11) Ethylene glycol distearate 1.0
(12) Hydroxypropyl methylcellulose 1% aqueous solution 5.0
(13) Sesame germinant extract of Production Example 1 0.01
(14) Perfume appropriate amount

[Example 3: lotion]
(Manufacturing method)
A. The following components (1) to (6) are mixed and dissolved.
B. The following components (7) to (12) are mixed and dissolved.
C. B was added to A and mixed to obtain a skin lotion.

(Ingredient) (%)
(1) Citric acid 0.05
(2) Sodium citrate 0.2
(3) Sodium pyrrolidonecarboxylate (50%) solution 0.5
(4) Dipotassium glycyrrhizinate 0.1
(5) Glycerin 3.0
(6) 1,3-butylene glycol 8.0
(7) Purified water remaining amount (8) Ethanol 10.0
(9) Sesame germinant extract of Production Example 2 2.0
(10) Perfume Appropriate amount (11) Preservative Appropriate amount (12) Polyoxyethylene monooleate (20E.O.)
Sorbitan 0.5

[Example 4: Latex (oil-in-water type)]
(Manufacturing method)
A. The following components (1) to (13) are dissolved by heating and maintained at 70 ° C.
B. The following components (14) to (19) are dissolved by heating and maintained at 70 ° C.
C. B is added to A to emulsify, and the following component (20) is further added and mixed.
D. C was cooled, the following component (21) was added and mixed to obtain an emulsion.

(Ingredient) (%)
(1) Stearic acid 1.0
(2) Cetanol 0.5
(3) Glycerol monostearate 0.5
(4) Liquid paraffin 2.0
(5) Squalane 3.0
(6) Jojoba oil 3.0
(7) Cetyl palmitate 0.2
(8) Retinol palmitate 0.2
(9) Tocopherol acetate 0.05
(10) Preservative appropriate amount (11) Sorbitan monostearate 0.3
(12) Polyoxyethylene monooleate (20E.O.)
Sorbitan 0.5
(13) Dibutylhydroxytoluene 0.1
(14) Triethanolamine 0.5
(15) 1,3-butylene glycol 15.0
(16) Glycerol 3.0
(17) Polyethylene glycol 6000 0.5
(18) Sesame germinant extract of Production Example 3 2.0
(19) Purified water remaining amount (20) 1% aqueous solution of carboxyvinyl polymer 8.0
(21) Perfume appropriate amount

[Example 5: Ointment]
(Manufacturing method)
A. A part of components (3), (4), (7) and (8) is heated and mixed and kept at 75 ° C.
B. Ingredients (1), (2), (6) are heated and mixed and maintained at 75 ° C.
C. Gradually add A to B.
D. (5) dissolved in the remainder of (8) was added while cooling C to obtain an ointment.

(Ingredient) (%)
(1) Stearic acid 15.0
(2) Cetanol 7.0
(3) Triethanolamine 2.0
(4) Glycerin 7.0
(5) Sesame germinated extract of Production Example 1 1.0
(6) Stearyl glycyrrhetinate 0.5
(7) D-Panthenol 1.0
(8) Purified water remaining

[Example 6: Cream]
(Manufacturing method)
A. The following components (1) to (14) are dissolved by heating and maintained at 70 ° C.
B. The following components (15) to (20) are dissolved by heating and maintained at 70 ° C.
C. B is added to A to emulsify, and the following component (21) is further added and mixed.
D. C was cooled, the following component (22) was added and mixed to obtain a cream.

(Ingredient) (%)
(1) Stearic acid 2.5
(2) Cetanol 2.5
(3) Glycerin monostearate 2.0
(4) Vaseline 2.0
(5) Dipentaerythritol fatty acid ester 2.0
(6) Isotridecyl myristate 5.0
(7) Liquid paraffin 8.0
(8) Squalane 5.0
(9) Beeswax 1.0
(10) Cetyl palmitate 2.0
(11) Sorbitan sesquioleate 0.5
(12) Polyoxyethylene monooleate (20E.O.)
Sorbitan 1.5
(13) Coenzyme Q10 0.3
(14) Preservative appropriate amount (15) Triethanolamine 1.2
(16) 1,3-butylene glycol 8.0
(17) Glycerin 2.0
(18) Polyethylene glycol 20000 0.5
(19) Sesame germinant extract of Production Example 2 0.01
(20) Purified water remaining amount (21) Carboxyvinyl polymer 1% aqueous solution 10.0
(22) Perfume appropriate amount

[Example 7: Water-in-oil type sunscreen cream]
(Manufacturing method)
A. The following components (1) to (8) were heated and mixed at 70 ° C.
B. The following components (9) to (12) and (14) to (15) were heated and mixed at 50 ° C.
C. B was added to A to emulsify, and after cooling, (13) was added to obtain a water-in-oil sunscreen cream.

(Ingredient) (%)
(1) Polyoxyalkylene-modified organopolysiloxane (Note 1) 2.0
(2) Octyl palmitate 15.0
(3) Decamethylcyclopentasiloxane 20.0
(4) Glyceryl tribehenate 1.0
(5) Fine zinc oxide 12.0
(6) Fine particle titanium oxide 3.0
(7) 2-Methylhexyl paramethoxycinnamate (Note 2) 7.0
(8) 4-tertbutyl-4′-methoxydibenzoylmethane
(Note 3) 1.0
(9) Dipropylene glycol 5.0
(10) Ethanol 5.0
(11) Polyethylene powder 3.0
(12) Preservative Appropriate amount (13) Fragrance Appropriate amount (14) Sesame seed extract of Production Example 3 0.1
(15) Remaining amount of purified water (Note 1) KF-6017 (manufactured by Shin-Etsu Chemical Co., Ltd.)
(Note 2) Yubinar MC80 (BASF)
(Note 3) PARSOL 1789 (manufactured by LC UNITED)

[Example 8: Pack cosmetic]
(Manufacturing method)
A. The following components (1) to (6) and (15) are heated and mixed at 70 ° C. and cooled to room temperature.
B. The following components (7) to (14) were added to A and mixed to obtain a pack cosmetic.

(Ingredient) (%)
(1) Polyvinyl alcohol 15.0
(2) Glycerin 10.0
(3) Polyoxyethylene methyl glucoside 3.0
(4) Glyceryl trioctanoate 5.0
(5) Stearyl glycyrrhetinate 0.1
(6) Sodium polyoxyethylene alkyl ether phosphate 1.0
(7) Ethanol 20.0
(8) Kaolin 2.0
(9) Titanium oxide 2.0
(10) Lactic acid (50% aqueous solution) 0.5
(11) Sodium lactate (50% aqueous solution) 0.5
(12) Preservative Appropriate amount (13) Fragrance Appropriate amount (14) Sesame germinated extract of Production Example 1 0.2
(15) Purified water remaining

[Example 9: Liquid foundation]
(Manufacturing method)
A. The following components (1) to (7) are heated and mixed at 70 ° C., and the following components (13) to (18) are added to the mixture and mixed to maintain at 70 ° C.
B. The following components (8) to (12) are heated and mixed at 70 ° C.
C. A was added to B to emulsify, and after cooling, the following components (19) to (20) were added to obtain a liquid foundation.

(Ingredient) (%)
(1) Dipentaerythritol fatty acid ester 2.0
(2) Liquid paraffin 5.0
(3) Stearic acid 2.0
(4) Cetanol 1.0
(5) Glyceryl monostearate 1.0
(6) 2-Methylhexyl paramethoxycinnamate 8.0
(7) Preservative appropriate amount (8) Glycerin 5.0
(9) Triethanolamine 1.0
(10) Carboxymethylcellulose 0.2
(11) Bentonite 0.5
(12) Purified water remaining amount (13) Titanium oxide 6.0
(14) Fine particle titanium oxide 2.0
(15) Fine zinc oxide 4.0
(16) Mica 2.0
(17) Talc 4.0
(18) Appropriate amount of color pigment (19) Sesame germinated extract of Production Example 2 1.0
(20) Perfume appropriate amount

The various cosmetics or external preparations for skin prepared as described above were excellent in the anti-aging effect by applying to the skin to improve the wrinkles and sagging of the skin and to make the skin beautiful and firm.

Claims (1)

Sesame (Pedaliaceae) Sesamum Sesame (Sesamuun)
indicum L.) An elastase activity inhibitor comprising, as an active ingredient, a sesame seed germinant extract extracted from a seed germinant.