JP2011132151A - Composition for improving lipid metabolism - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a composition for improving lipid metabolism that contains as an effective ingredient a component derived from a natural product such as a plant and exhibits mild and sure effects for the improvement of lipid metabolism such as suppression of fat accumulation or suppression of body weight increase. <P>SOLUTION: The composition for improving lipid metabolism contains as effective ingredients resveratrol and hardly digestible dextrin. The composition for improving lipid metabolism exhibits mild actions by the component derived from a natural product and sure lipid metabolism-improving actions in suppression of fat accumulation or suppression of body weight increase by a combination of components thereby to be a daily ingestible composition for improving lipid metabolism. <P>COPYRIGHT: (C)2011,JPO&INPIT

Description

  The present invention comprises resveratrol and indigestible dextrin as active ingredients, a gentle action of ingredients derived from natural products, a fat accumulation suppression by a combination of ingredients, a reliable lipid metabolism improvement action by a synergistic effect in weight gain inhibition The present invention relates to a composition for improving lipid metabolism.

  Obesity is one of the causes of metabolic syndrome associated with hypertension, hyperlipidemia, hyperglycemia, etc., and metabolic syndrome is known to increase the risk of arteriosclerosis. In Japan, the increase in the number of people with metabolic syndrome and those who are considered reserves has been recognized for some time, and it is important to take measures in the “Health Japan 21 (National Health Promotion Movement in the 21st Century)” launched in 1998. Sex is pointed out. However, despite the “recommendation of exercise and appropriate dietary habits” promoted in Health Japan 21, BMI (physical index) continues to increase, especially among middle-aged and older men, and the proportion of energy intake from fat is also 40- The price is as high as 50% (Ministry of Health, Labor and Welfare, 2004 National Health and Nutrition Survey Summary).

  As described above, appropriate energy intake and exercise are important for the prevention of obesity, but drug therapy may be used in the case of “obesity” requiring medical weight loss treatment. As therapeutic agents for obesity currently in use, appetite suppressants such as mazindol and sibutramine and carbohydrase inhibitors such as acarbose are known. In addition, orlistat is known as a pancreatic lipase inhibitor that suppresses absorption of fat in the intestinal tract, but any drug has been reported to have side effects and is not necessarily safe (instruction manual for obesity / obesity, Ishiyaku Publishing, 2003). Limiting calorie intake is an effective means for preventing obesity, but it is often difficult to carry out in daily life. Therefore, moderately suppressing the absorption of nutritional components contained in meals into the body is considered to be a useful method for preventing obesity and metabolic syndrome.

  In view of the above, in recent years, attempts to prevent obesity by suppressing absorption of carbohydrates and lipids have been studied, and foods that are expected to be effective in suppressing absorption of carbohydrates and lipids. The functional ingredients involved have gained attention. Various kinds of functional ingredients contained in foods for this purpose are disclosed. For example, many plant-derived components have been reported as substances that suppress the increase in blood lipid concentration after meals. Examples of such plant-derived components include tannins prepared by extraction and purification from Kawara ketsumei (JP-A-8-259557), lemongrass, allspice, cinnamon or clove plants or extracts thereof (special (Kai 2001-120237), chitin-chitosan (Int. J. Obes. Relat Metab Disord. 23: 174-179, 1999), pepper, pumpkin, shimeji, maitake, hijiki, green tea, black tea, oolong tea An article is disclosed (Japanese Patent Laid-Open No. 3-219872). Furthermore, indigestible dextrin, which is a water-soluble dietary fiber derived from plants, has been reported ("Japan Dietary Fiber Research Society" Vol.4, No.2, 2000).

  On the other hand, resveratrol (t-resveratrol: 3,4 ', -5-Trihydroxy-stillben), a kind of polyphenol, is a phytoalexin (antibacterial substance) that has an antioxidant action in grapes and red wine. It is. This substance has been shown to prevent and improve heart disease, antioxidant activity, anti-inflammatory effect, prevention of cerebral thrombosis, anticancer effect, and inhibition of brain tissue damage after cerebral infarction. An anti-obesity effect has been shown.

  Resveratrol is known to be contained in peanuts and Japanese knotweeds in addition to grapes, but JP 2005-281179 concentrates and collects high-level resveratrol from the roots of knotweeds. A method is disclosed and formulation into formulations such as cosmetics is shown. Japanese Patent Laid-Open No. 2006-274334 discloses that resveratrol extracted from grapes or the like is used as a lipid metabolism activator as a substance having a lipid metabolism promoting action by AMPK (AMP-activated protein kinase) activation. JP 2008-239576 A discloses that resveratrols extracted from grape buds and vines are used as a body fat reducing agent. Furthermore, JP 2009-13159 A discloses an extract from cowberry fruit containing anthocyanidins, proanthocyanidins and resveratrol, preventing dandruff, dieting against caloric intake, removal of antioxidant free radicals, It is disclosed that it is used for functional foods intended for prevention of cerebrovascular diseases, suppression of thrombus formation, and whitening.

  As mentioned above, various plant-derived components have been found as substances that suppress the absorption of carbohydrates and lipids for the purpose of improving health function, but the greatest aim of using these plant-derived components is Therefore, it is to provide a substance having a gentle action and a reliable health function. Therefore, it is desired to search for a further plant component having such an action or function.

JP-A-3-219872 JP-A-8-259557 JP 2001-120237 A JP 2005-281179 A JP 2006-273434 A JP 2008-239576 A JP 2009-13159 A

Obesity / obesity instruction manual, Ishiyaku Publishing, 2003 Int. J. Obes. Relat Metab Disord. 23: 174-179, 1999 Journal of Japan Dietary Fiber Research Association, Vol.4, No.2, 2000

  An object of the present invention is to use a component derived from a natural product such as a plant as an active ingredient, and in a lipid metabolism improving action such as fat accumulation inhibition and weight gain inhibition, the lipid metabolism exhibiting a mild action and a sure lipid metabolism improving action It is to provide a composition for improvement.

  The present inventor has been diligently examining the physiological activity of resveratrol (3,4 ', 5-Trihydroxy-stillbene), and the combination of resveratrol and indigestible dextrin is effective in suppressing fat accumulation and weight gain. In the lipid metabolism improving action such as suppression, it has been found that there is a synergistic effect, and by combining such active ingredients, it has been found that there is an excellent lipid metabolism improving action than when each component is used alone, The present invention has been completed. That is, this invention consists of a composition for lipid metabolism improvement containing resveratrol and indigestible dextrin as an active ingredient. The composition for improving lipid metabolism of the present invention is for improving lipid metabolism that can be ingested on a daily basis by a gentle action of components derived from natural products, a fat accumulation suppression by combination of ingredients, and a reliable lipid metabolism improvement action in suppressing weight gain. A composition is provided.

  In the composition for improving lipid metabolism of the present invention, the content ratio of resveratrol and indigestible dextrin is preferably 1:10 to 1:20 by weight. The dosage form of the composition for improving lipid metabolism of the present invention is not particularly limited, but it is usually desirable to administer in the form of oral administration, and it is preferred to administer in the form of continuous administration because of its effect. Moreover, the composition for improving lipid metabolism of the present invention can be administered (taken) in the form of a food or drink.

  Specifically, the present invention is (1) a composition for improving lipid metabolism containing resveratrol and indigestible dextrin as active ingredients, and (2) a composition for improving lipid metabolism is a composition for inhibiting weight gain. (1), wherein the composition for improving lipid metabolism according to (1) above or (3) the composition for improving lipid metabolism is a composition for inhibiting fat accumulation The composition for improving lipid metabolism described in the above, or (4) the above-mentioned (1) wherein the content ratio of resveratrol and indigestible dextrin in the composition is 1:10 to 1:20 by weight ratio ( It consists of the composition for lipid metabolism improvement in any one of 1)-(3).

  The present invention uses a component derived from a natural product such as a plant as an active ingredient, and for lipid metabolism improvement that has a mild action and a reliable lipid metabolism improvement action in lipid metabolism improvement actions such as fat accumulation inhibition and weight gain inhibition. A composition is provided. The composition for improving lipid metabolism of the present invention provides a lipid metabolism improving agent that can be taken on a daily basis.

In the results of the test on the lipid metabolism improving effect of resveratrol and indigestible dextrin in the high-fat diet mixed diet and the standard diet mixed diet test in Examples of the present invention, the total food intake [control group ( HFD); resveratrol 0.2% group (0.2% RES); indigestible dextrin 3% (w / w) group (3% dexterity); resveratrol 0.2% and indigestible dextrin It is a figure which shows the total food intake (3 animals) (g / cage)] for 67 days in a 3% (w / w) group (0.2% RES + 3% hard dexterity). In the examples of the present invention, the daily body weight (g) of each group of the test plots in the results of the test on the lipid metabolism improving effect of resveratrol and indigestible dextrin in the high-fat diet mixed diet and the standard diet mixed diet test FIG. In the examples of the present invention, the fat weight around the testis per body weight of each group of the test plots in the results of the test on the lipid metabolism improving effect of resveratrol and indigestible dextrin in the high fat diet and standard diet diet test It is a figure which shows (mg / g body weight). In the examples of the present invention, the fat around the intestinal tract per body weight of each group in the test section in the results of the test on the lipid metabolism improving effect of resveratrol and indigestible dextrin in the high-fat diet mixed diet and the standard diet mixed diet test It is a figure which shows weight (mg / g body weight). In the examples of the present invention, the fat weight around the kidney per body weight of each group of the test plots in the results of the test on the lipid metabolism improving effect of resveratrol and indigestible dextrin in the high fat diet mixed diet and the standard diet mixed diet test It is a figure which shows (mg / g body weight). In the results of the test on the lipid metabolism-improving effect of resveratrol and indigestible dextrin in the high-fat diet mixed diet and the standard diet mixed diet test in the examples of the present invention, the weight of subcutaneous fat (mg / G body weight).

  The present invention comprises a composition for improving lipid metabolism having resveratrol and indigestible dextrin as active ingredients and having a fat accumulation inhibitory action and a weight gain inhibitory action.

  Resveratrol (3,4 ′, 5-Trihydroxy-stillbene) used in the present invention is stilbenetriol which is a kind of polyphenol. Resveratrol is a polyphenol that is readily soluble in ethyl alcohol and exists in trans and cis forms, and pie seed is a glycoside of resveratrol.

  In the present invention, resveratrol can be obtained by synthesis or biosynthesis, or resveratrol or pie seed-containing plant or food can be used as it is, or it can be cut, crushed, naturally dried, hot-air dried, or frozen. What was processed by drying and fermentation, and what was obtained by performing processing, such as extraction, concentration, pulverization, and granulation, can also be used according to a conventional method. Examples thereof include grapes, peanuts, peanuts, and teraceae plants such as Japanese knotweed and crested damselfly.

  Grape is particularly preferable as a collection source of resveratrol used as an active ingredient in the present invention. The type of grape is not particularly limited, but Delaware, Kyoho, Koshu, Pione, Muscat, Chenin Blanc, Grenache, Mataro, Muller Thurgau, Trebbiano, Berry A, Cabernet Sauvignon, Merlot, Pinot Noir, Cabernet Franc, Schiller, Chardonnay, Sauvignon Blanc, Semillon, Schiller, Gamay, Riesling, Alligote and the like are preferred. A grape extract refers to an extract from a product derived from grape berries, grape leaves or grapes. The thing derived from grape means grape juice, wine, the residue at the time of wine manufacture, wine concentrate, etc.

  Resveratrol used as an active ingredient in the present invention can be prepared by extraction from the above natural product-derived material. For example, since resveratrol is contained in a large amount in grape skins and leaves, extraction is performed by a general extraction method using water, hot water, alcohol water, an organic solvent, or the like. Moreover, since it is contained also in wine, the concentrate of wine, or the solid substance solidified to dryness, they can also be used. Further, the residue of grapes generated during the production of wine can be used as it is, or extracted with an organic solvent / water or the like. As the organic solvent, alcohol such as ethanol is preferable, and ethanol is particularly preferable.

  The indigestible dextrin used as an active ingredient in the present invention is defined as a dextrin that is not hydrolyzed by human digestive enzymes. More specifically, glucose which is nutritive in humans is a structural unit, and at least 3 or more molecules of glucose are linked by glycoside bonds, and further has glycoside bonds that are not hydrolyzed by human digestive enzymes. Moreover, as long as it contains a hardly digestible dextrin in the degradation product hydrolyzed by the human digestive enzyme, it may have a glycoside bond hydrolyzed by the human digestive enzyme. Therefore, it has a glycoside bond that is hydrolyzed by a human digestive enzyme before ingestion by a human, and the hydrolyzed glycoside bond is hydrolyzed by a human digestive enzyme before it is transferred from the stomach to the small intestine. Even if it is decomposed, it is included in the indigestible dextrin as referred to in the present invention as long as the decomposed product contains the indigestible dextrin. It may be produced from starch such as rice cake and marketed.

  In the formulation of the lipid metabolism improving composition of the present invention, in addition to the active ingredients composed of resveratrol and indigestible dextrin, additives suitable for each application can be appropriately contained. Examples of the additive include amino acids such as valine, leucine, isoleucine, arginine, lysine, glutamine, alanine, serine, glycine, cysteine, and threonine.

  The composition of the present invention can be made into a composition more suitable for improving lipid metabolism by appropriately containing other components in addition to the active components composed of resveratrol and indigestible dextrin components. Examples of the other components include wheat-derived components, malt, wort, hops, hop extract, fresh tea leaf extract, brown rice, hard wheat, hub tea, corn, collagen peptide, deep sea water for drinking, and dried rose. Hips, black beans, black sesame seeds, citrus aloe, yuzu peel, vitamins, vitamin C, spice extract, tea, caffeine, fructose glucose liquid sugar, salt, alanine, citrulline, organic acids, citric acid, sodium citrate, calcium lactate Arginine, iron pyrophosphate, calcium gluconate, potassium chloride, magnesium chloride, sugar, acidulant, turmeric, fermented turmeric extract, curcumin, skim milk powder, milk protein, milk peptide, cyclodextrin and the like. The composition for improving lipid metabolism of the present invention contains an active ingredient composed of resveratrol and indigestible dextrin components, and optionally one or more pharmacologically acceptable carriers, and further necessary Depending on the case, it may contain other active ingredients for treatment.

  The composition for improving lipid metabolism of the present invention may be prepared by mixing an active ingredient composed of resveratrol and indigestible dextrin components together with a carrier, etc., if necessary. It can be formulated by a method. The dosage form of the preparation is desirably the most effective in the treatment, and oral administration or parenteral administration such as intravenous, intraperitoneal or subcutaneous administration is preferable, but oral administration is more preferable. . Examples of dosage forms to be administered include tablets, powders, granules, pills, suspensions, emulsions, soaking / decoction, capsules, syrups, solutions, elixirs, extracts, tinctures, fluid extracts, etc. Any of parenteral preparations such as oral preparations, injection preparations, instillation preparations, cream preparations, and suppositories may be used, but they are preferably used as oral preparations.

  In formulating the composition for improving lipid metabolism of the present invention, excipients, binders, disintegrants, lubricants, dispersants, suspension agents, emulsifiers, diluents, buffers, antioxidants, Additives such as bacterial inhibitors can be used. In the case of liquid preparations such as syrups suitable for oral administration, sugars such as water, sucrose, sorbitol and fructose, glycols such as polyethylene glycol and propylene glycol, oils such as sesame oil, olive oil and soybean oil Preservatives such as p-hydroxybenzoic acid esters, and flavors such as strawberry flavor and peppermint can be added to prepare a preparation.

  In the case of tablets, powders, granules, etc. suitable for oral administration, sugars such as lactose, sucrose, glucose, sucrose, mannitol, sorbitol, starch such as potato, wheat, corn, calcium carbonate, calcium sulfate , Inorganic substances such as sodium bicarbonate, sodium chloride, excipients such as plant powder such as licorice powder, gentian powder, starch, agar, gelatin powder, crystalline cellulose, carmellose sodium, carmellose calcium, calcium carbonate, sodium bicarbonate , Disintegrants such as sodium alginate, lubricants such as magnesium stearate, talc, hydrogenated vegetable oil, macrogol, silicone oil, polyvinyl alcohol, hydroxypropylcellulose, methylcellulose, ethylcellulose, carmellose, gelatin, starch paste, etc. Join , Surfactants such as fatty acid esters, by adding a plasticizer such as glycerin, can be formulated.

  In addition, preparations suitable for oral administration include additives generally used in foods and drinks such as sweeteners, coloring agents, preservatives, thickening stabilizers, antioxidants, color formers, bleaches, fungicides, gum bases. Bitterings, enzymes, brighteners, acidulants, seasonings, emulsifiers, fortifiers, manufacturing agents, fragrances, spice extracts and the like may be added.

  Suitable formulations for oral administration are as is or for example beer, fermented alcoholic beverages, beer-taste beverages, juices, soft drinks, teas, lactic acid bacteria beverages, fermented milk, frozen desserts, butter, cheese, yogurt, processed milk, defatted Dairy products such as milk, livestock meat products such as ham, sausage, hamburger, fish paste products such as salmon, bamboo rings, and fish cakes, egg products such as broiled and egg tofu, cookies, jelly, chewing gum, candy, snacks and other confectionery Bread, noodles, pickles, salmon products, dried fish, boiled, salted products, soups, seasonings, etc., powdered foods, sheet foods, bottled foods, canned foods, retort foods, capsule foods, tablet foods, liquid foods , Used as food and drink such as health food for improving lipid metabolism, functional food, dietary supplement, food for specified health It may be.

  For example, injections suitable for parenteral administration preferably comprise a sterile aqueous solution containing ornithine or a salt thereof that is isotonic with the blood of the recipient. For example, in the case of an injection, a solution for injection is prepared using a carrier such as a salt solution, a glucose solution, or a mixture of a salt solution and a glucose solution. Also in these parenteral agents, one or more selected from the diluents, preservatives, flavors, excipients, disintegrants, lubricants, binders, surfactants, plasticizers and the like exemplified for oral agents Further auxiliary components may be added.

  Resveratrol in the composition for improving lipid metabolism of the present invention is appropriately selected according to the type of preparation, the effect expected by administration of the preparation, etc., but usually 0.01 to 5.0% by weight, Preferably it is 0.05 to 1.0 weight%, Most preferably, it is 0.1 to 0.5 weight%. Indigestible dextrin is usually 0.15 to 75.0% by weight, preferably 0.75 to 15.0% by weight, particularly preferably 1.5 to 7.5% by weight. The dosage and frequency of administration of the preparation of the present invention vary depending on the dosage form, age of the person to be administered, body weight, etc., but usually 0.1 g to 10 g as a resveratrol per day for an adult, preferably Is generally from 1.5 g to 150 g, preferably from 3.0 g to 60.0 g, particularly preferably from 0.5 g to 4.0 g, particularly preferably from 0.5 g to 2.0 g. Dosage once to several times a day to give 7.5 to 30.0 g. The administration period is not particularly limited, but is usually 1 day to 1 year, preferably 1 day to 3 months.

  The ratio of resveratrol and indigestible dextrin in the composition of the present invention is 1: 2 to 1: 100, preferably 1: 5 to 1:40, more preferably 1:10 to 1 by weight. : 20. Lipid metabolism can be improved by administering or ingesting the medicament or food or drink of the present invention. In the present invention, the lipid metabolism improving action is specifically due to actions such as lipid accumulation suppression, and these also constitute a part of the use of the present invention. The weight gain inhibitory action is due to the action of inhibiting fat accumulation, in particular, inhibiting visceral fat accumulation, which also constitutes part of the application of the present invention.

  EXAMPLES Hereinafter, although an Example is given and this invention is demonstrated further more concretely, this invention is not limited to these Examples.

[Improvement of lipid metabolism by resveratrol and indigestible dextrin in high-fat diet and standard diet studies]
<Test method>
Five-week-old female C57BL / 6J mice (Nippon Charles River) were bred with AIN93G feed (Oriental yeast) for 7 days under free drinking and free feeding, and then feeding of the test food was started. The test meal is a high-fat meal (Metabolism, 45, 1539-1546 (1996)) with a lipid-derived energy ratio of 60%. Resveratrol, indigestible dextrin, resveratrol + indigestible dextrin administration group Each was fed with a combination of 0.2% resveratrol, 3% resistant dextrin, or 0.2% resveratrol and 3% resistant to dextrin (w / w). All groups were subjected to a 67-day feeding test at n = 9. During the breeding period, food intake was measured twice a week, and body weight was measured once a week. On the last day of breeding, the animals were euthanized by cervical dislocation after ether anesthesia under non-fasting conditions. Thereafter, dissection was performed, and subcutaneous adipose tissue, peri-testicular adipose tissue, peri-renal adipose tissue, and peri-mesenteric adipose tissue were collected, and wet weight was measured. Significant difference test was performed by Mann-Whitney U test, and it was considered significant when p <0.05 or less.

<Result>
The results are shown in FIGS. FIG. 1 shows the total food consumption of each group of the test plots [control group (HFD); resveratrol 0.2% group (0.2% RES); indigestible dextrin 3% (w / w) group (3 67% total food intake (3 animals) in resveratrol 0.2% and indigestible dextrin 3% (w / w) group (0.2% RES + 3% difficult dex) (g) / Cage)]. FIG. 2 shows changes in body weight (g) over time for each group in the test plot. FIG. 3 shows fat weight around the testis per body weight (mg / g body weight) of each group in the test plot, and FIG. ), FIG. 5 shows fat weight around the kidney (mg / g body weight) per body weight of each group in the test group, and FIG. 6 shows subcutaneous fat weight (mg / g body weight) per body weight in the test group. Indicates.

  In the above test, there was no change in the amount of food consumed in each experimental group during the breeding period. As shown in the figure, both the resveratrol 0/2% group and the indigestible dextrin 3% group showed lower values than the control group, but resveratrol 0.2% + indigestible It remained at a lower value with a 3% dextrin diet. After 7 weeks from the start of the feeding, only the resveratrol 0.2% + indigestible dextrin 3% feeding group was significantly lower than the control group. Furthermore, the weight of the adipose tissue at the time of dissection was lower than that of the control group in each of the subcutaneous, peri-renal, peri-testicular, and mesenteric areas, but resveratrol 0.2% + indigestion The group fed with 3% sex dextrin showed a lower value than the group administered alone.

  From this result, it was suggested that the inhibitory effect on body weight gain by administration of dietary resveratrol 0.2% + indigestible dextrin 3% is due to suppression of fat accumulation. From the above results, suppression of body weight gain and suppression of fat accumulation were shown, and simultaneous intake of resveratrol 0.2% + indigestible dextrin 3% has a synergistic effect of each component. It has been shown to be more effective than obesity prevention alone.

The present invention uses a component derived from a natural product such as a plant as an active ingredient, and in a lipid metabolism improving action such as fat accumulation suppression and weight gain suppression, it can be ingested on a daily basis, with a gentle action and a reliable lipid metabolism improvement. Provided is a composition for improving lipid metabolism that exerts an action.

Claims (4)

  A composition for improving lipid metabolism comprising resveratrol and indigestible dextrin as active ingredients.   The composition for improving lipid metabolism according to claim 1, wherein the composition for improving lipid metabolism is a composition for suppressing weight gain.   The composition for improving lipid metabolism according to claim 1, wherein the composition for improving lipid metabolism is a composition for suppressing fat accumulation.   The content ratio of resveratrol and indigestible dextrin in the composition is 1:10 to 1:20 by weight ratio, for improving lipid metabolism according to any one of claims 1 to 3 Composition.