JP2011043332A - 蛍光検出器 - Google Patents
蛍光検出器 Download PDFInfo
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- JP2011043332A JP2011043332A JP2009189666A JP2009189666A JP2011043332A JP 2011043332 A JP2011043332 A JP 2011043332A JP 2009189666 A JP2009189666 A JP 2009189666A JP 2009189666 A JP2009189666 A JP 2009189666A JP 2011043332 A JP2011043332 A JP 2011043332A
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- 238000005259 measurement Methods 0.000 claims abstract description 25
- 230000004907 flux Effects 0.000 claims abstract description 9
- 230000003287 optical effect Effects 0.000 claims description 47
- 230000005284 excitation Effects 0.000 claims description 44
- 230000007246 mechanism Effects 0.000 claims description 2
- 230000004044 response Effects 0.000 claims description 2
- 238000001917 fluorescence detection Methods 0.000 claims 1
- 239000000523 sample Substances 0.000 abstract description 79
- 239000012488 sample solution Substances 0.000 abstract description 14
- 230000035945 sensitivity Effects 0.000 abstract description 4
- 238000010521 absorption reaction Methods 0.000 abstract description 3
- 238000011088 calibration curve Methods 0.000 description 11
- 239000007788 liquid Substances 0.000 description 8
- 238000010586 diagram Methods 0.000 description 5
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 239000000470 constituent Substances 0.000 description 2
- 238000004401 flow injection analysis Methods 0.000 description 2
- 239000011347 resin Substances 0.000 description 2
- 229920005989 resin Polymers 0.000 description 2
- AKYHKWQPZHDOBW-UHFFFAOYSA-N (5-ethenyl-1-azabicyclo[2.2.2]octan-7-yl)-(6-methoxyquinolin-4-yl)methanol Chemical group OS(O)(=O)=O.C1C(C(C2)C=C)CCN2C1C(O)C1=CC=NC2=CC=C(OC)C=C21 AKYHKWQPZHDOBW-UHFFFAOYSA-N 0.000 description 1
- 239000001576 FEMA 2977 Substances 0.000 description 1
- 238000007792 addition Methods 0.000 description 1
- 230000002238 attenuated effect Effects 0.000 description 1
- 238000005452 bending Methods 0.000 description 1
- LOUPRKONTZGTKE-UHFFFAOYSA-N cinchonine Natural products C1C(C(C2)C=C)CCN2C1C(O)C1=CC=NC2=CC=C(OC)C=C21 LOUPRKONTZGTKE-UHFFFAOYSA-N 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000012921 fluorescence analysis Methods 0.000 description 1
- 230000001678 irradiating effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000002093 peripheral effect Effects 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 229960003110 quinine sulfate Drugs 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
- G01J3/28—Investigating the spectrum
- G01J3/44—Raman spectrometry; Scattering spectrometry ; Fluorescence spectrometry
- G01J3/4406—Fluorescence spectrometry
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
- G01J3/02—Details
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
- G01J3/02—Details
- G01J3/0205—Optical elements not provided otherwise, e.g. optical manifolds, diffusers, windows
- G01J3/0208—Optical elements not provided otherwise, e.g. optical manifolds, diffusers, windows using focussing or collimating elements, e.g. lenses or mirrors; performing aberration correction
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
- G01J3/02—Details
- G01J3/0205—Optical elements not provided otherwise, e.g. optical manifolds, diffusers, windows
- G01J3/0229—Optical elements not provided otherwise, e.g. optical manifolds, diffusers, windows using masks, aperture plates, spatial light modulators or spatial filters, e.g. reflective filters
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N2021/6417—Spectrofluorimetric devices
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N2021/6463—Optics
- G01N2021/6467—Axial flow and illumination
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- Physics & Mathematics (AREA)
- Spectroscopy & Molecular Physics (AREA)
- General Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Optical Measuring Cells (AREA)
Abstract
【解決手段】測定対象の試料の濃度が高いことが分かっている場合には、励起光Lex通過方向に短い開口6bの光束制限部6を用い、励起光入射端部に近い領域から発した蛍光のみを集光レンズ7で集光して蛍光側分光器4に導入して検出する。この場合、試料液Sで強い吸収を受けた励起光が通過する領域から発する蛍光は測定結果に反映されないので、蛍光量は減るものの濃度と蛍光強度との関係の直線性が良好になり、高い濃度での定量精度が向上する。試料濃度が低い場合には、感度を重視して励起光Lex通過方向に長い開口6aの光束制限部6を用いればよい。
【選択図】図1
Description
前記試料セル中での励起光の通過方向に所定長さの開口を有し、試料から放出された蛍光のうち前記開口を通過しない蛍光を遮るべく前記試料セルに近接して配設される光束制限手段と、該光束制限手段の開口を通過した蛍光を集光して前記蛍光測定光学系に導入する集光手段と、を一対とする蛍光調整光学系を有し、
前記開口の長さを少なくとも2種類以上に選択可能であるように前記蛍光調整光学系を交換する又は切り替える機構を備えることを特徴としている。
2…励起側分光器
3…フローセル
4…蛍光側分光器
5…検出器
6…光束制限部
6a、6b…開口
7…集光レンズ
S…試料液
10…セルハウジング
11…集光レンズ
12…凹面鏡
20…蛍光調整光学ユニット
21…レンズホルダ
21a…底面板
21b…孔
22…Oリング
Claims (3)
- 試料を内部に保持する試料セルと、該試料セルに励起光を照射する励起光学系と、該励起光に対して試料から放出される蛍光を検出する蛍光測定光学系と、を具備する蛍光検出器において、
前記試料セル中での励起光の通過方向に所定長さの開口を有し、試料から放出された蛍光のうち前記開口を通過しない蛍光を遮るべく前記試料セルに近接して配設される光束制限手段と、該光束制限手段の開口を通過した蛍光を集光して前記蛍光測定光学系に導入する集光手段と、を一対とする蛍光調整光学系を有し、
前記開口の長さを少なくとも2種類以上に選択可能であるように前記蛍光調整光学系を交換する又は切り替える機構を備えることを特徴とする蛍光検出器。 - 請求項1に記載の蛍光検出器であって、
前記光束制限手段は、開口長に依らず、前記試料セルへの励起光の入射端部側から放出される蛍光が通過可能なような開口を有することを特徴とする蛍光検出器。 - 請求項2に記載の蛍光検出器であって、
前記蛍光調整光学系は一体化された部材であり、その部材を交換することにより前記開口の長さを少なくとも2種類以上に選択可能としたことを特徴とする蛍光検出器。
Priority Applications (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2009189666A JP5272965B2 (ja) | 2009-08-19 | 2009-08-19 | 蛍光検出器 |
US12/851,440 US8253117B2 (en) | 2009-08-19 | 2010-08-05 | Fluorescence detector |
CN2010102552840A CN101995399B (zh) | 2009-08-19 | 2010-08-13 | 荧光检测器 |
Applications Claiming Priority (1)
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JP2009189666A JP5272965B2 (ja) | 2009-08-19 | 2009-08-19 | 蛍光検出器 |
Publications (2)
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JP2011043332A true JP2011043332A (ja) | 2011-03-03 |
JP5272965B2 JP5272965B2 (ja) | 2013-08-28 |
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JP2009189666A Active JP5272965B2 (ja) | 2009-08-19 | 2009-08-19 | 蛍光検出器 |
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US (1) | US8253117B2 (ja) |
JP (1) | JP5272965B2 (ja) |
CN (1) | CN101995399B (ja) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2016151526A (ja) * | 2015-02-18 | 2016-08-22 | アズビル株式会社 | フローセル及びフローセルの製造方法 |
US11408818B2 (en) | 2017-03-17 | 2022-08-09 | Shimadzu Corporation | Flow cell unit |
Families Citing this family (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR101482372B1 (ko) * | 2011-06-29 | 2015-01-13 | 파나소닉 주식회사 | 발광 소자의 제조 방법 및 발광 소자의 제조 장치 |
JP5939781B2 (ja) | 2011-12-09 | 2016-06-22 | 日本分光株式会社 | 高耐圧フローセル、フローセルアッセンブリ、蛍光検出器および超臨界流体クロマトグラフ |
CN103487375A (zh) * | 2013-09-24 | 2014-01-01 | 东南大学 | 基于白光led的三色荧光检测仪 |
CN105424634A (zh) * | 2015-10-29 | 2016-03-23 | 中国计量学院 | 一种基于光纤耦合紫外光源的水质cod检测器及其预测模型优化系统 |
KR20200025948A (ko) * | 2018-08-31 | 2020-03-10 | 삼성전자주식회사 | 광 필터 및 이를 포함하는 분광기 |
CN112903644B (zh) * | 2021-01-21 | 2021-11-26 | 上海交通大学 | 一种超宽范围的荧光定量分析方法和荧光测量系统 |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
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JPH05223738A (ja) * | 1992-02-14 | 1993-08-31 | Res Dev Corp Of Japan | 蛍光断層像測定装置 |
JPH10115584A (ja) * | 1996-10-11 | 1998-05-06 | Dkk Corp | 蛍光フローセル |
JPH10246692A (ja) * | 1997-02-28 | 1998-09-14 | Shimadzu Corp | 蛍光検出器 |
JP2008249667A (ja) * | 2007-03-30 | 2008-10-16 | Gl Sciences Inc | 蛍光検出装置 |
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US5529679A (en) * | 1992-02-28 | 1996-06-25 | Hitachi, Ltd. | DNA detector and DNA detection method |
JP3748571B2 (ja) * | 1996-11-18 | 2006-02-22 | ノバルティス アクチエンゲゼルシャフト | 測定装置及びその使用方法 |
US6316774B1 (en) * | 1998-08-18 | 2001-11-13 | Molecular Devices Corporation | Optical system for a scanning fluorometer |
DE19923822A1 (de) * | 1999-05-19 | 2000-11-23 | Zeiss Carl Jena Gmbh | Scannende Anordnung, vorzugsweise zur Erfassung von Fluoreszenzlicht |
CA2439307A1 (en) * | 2001-02-23 | 2002-09-06 | Genicon Sciences Corporation | Methods for providing extended dynamic range in analyte assays |
JP4834242B2 (ja) * | 2001-05-30 | 2011-12-14 | オリンパス株式会社 | 蛍光読み取り装置 |
CN1167945C (zh) * | 2001-08-07 | 2004-09-22 | 中国科学院大连化学物理研究所 | 一种激光诱导荧光检测器 |
AU2002951841A0 (en) * | 2002-09-30 | 2002-10-24 | Swinburne University Of Technology | Apparatus |
CN1566910A (zh) * | 2003-07-10 | 2005-01-19 | 上海复生生物工程研究所有限公司 | 荧光检测仪 |
US7095500B2 (en) * | 2004-01-30 | 2006-08-22 | Nalco Company | Interchangeable tip-open cell fluorometer |
JP4661768B2 (ja) | 2006-11-08 | 2011-03-30 | 株式会社島津製作所 | 蛍光測定用セル装置及び蛍光検出器 |
-
2009
- 2009-08-19 JP JP2009189666A patent/JP5272965B2/ja active Active
-
2010
- 2010-08-05 US US12/851,440 patent/US8253117B2/en active Active
- 2010-08-13 CN CN2010102552840A patent/CN101995399B/zh active Active
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH05223738A (ja) * | 1992-02-14 | 1993-08-31 | Res Dev Corp Of Japan | 蛍光断層像測定装置 |
JPH10115584A (ja) * | 1996-10-11 | 1998-05-06 | Dkk Corp | 蛍光フローセル |
JPH10246692A (ja) * | 1997-02-28 | 1998-09-14 | Shimadzu Corp | 蛍光検出器 |
JP2008249667A (ja) * | 2007-03-30 | 2008-10-16 | Gl Sciences Inc | 蛍光検出装置 |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2016151526A (ja) * | 2015-02-18 | 2016-08-22 | アズビル株式会社 | フローセル及びフローセルの製造方法 |
US11408818B2 (en) | 2017-03-17 | 2022-08-09 | Shimadzu Corporation | Flow cell unit |
Also Published As
Publication number | Publication date |
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US8253117B2 (en) | 2012-08-28 |
CN101995399A (zh) | 2011-03-30 |
JP5272965B2 (ja) | 2013-08-28 |
US20110042581A1 (en) | 2011-02-24 |
CN101995399B (zh) | 2012-10-17 |
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