JP2011020990A - Magnolia obovata extract, method for producing the same, and skin-beautifying composition containing the same - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide Magnolia obovata extract having a skin-beautifying effect superior to conventional Magnolia obovata extract obtained by extracting Magnolia obovata with water, alcohol, or the like. <P>SOLUTION: The Magnolia obovata extract is prepared by extracting Magnolia obovata with supercritical carbon dioxide. <P>COPYRIGHT: (C)2011,JPO&INPIT

Description

  The present invention relates to a honoki extract obtained by extracting honoki with supercritical carbon dioxide, a method for producing the same, and a skin beautifying composition containing the extract.

The skin consists of three layers of epidermis, dermis and subcutaneous tissue, and among these, the dermis is rich in hyaluronic acid which is important for maintaining skin functions such as skin elasticity and moisture retention. This hyaluronic acid is produced by fibroblasts present in the dermis.
On the other hand, when the function of fibroblasts deteriorates due to stress such as aging or ultraviolet rays, the production of hyaluronic acid in the dermis is reduced. As a result, skin aging phenomena such as skin wrinkles, sagging, and decreased skin functions such as moisture retention and elasticity appear.

  Furthermore, in skin exposed to sunlight, particularly ultraviolet rays, melanin is produced in melanin-producing vesicles called melanosomes of melanocytes (pigment cells) present in the epidermis. And it is thought that pigmentation of skin, such as a spot, a buckwheat, and a liver spot, arises when the produced melanin is delivered to an epidermal cell. Moreover, these pigmentation generate | occur | produces with aging, and it becomes difficult to lose | disappear as it increases.

Accordingly, there is a strong demand for the development of substances and compositions having an effect of preventing and / or improving such pigmentation and skin aging, that is, a skin beautifying effect.
In the past, for example, for the purpose of preventing and / or improving skin aging, a composition containing sugar, amino acid, organic acid, pyrrolidone carboxylic acid, etc., or hyaluronic acid to supplement lost hyaluronic acid Compositions and the like have been developed.
However, these compositions are not satisfactory because they improve skin moisturization and improve the keratinous state of the epidermis, and do not fundamentally prevent or improve skin aging. It was.

In addition, as a substance having a so-called whitening effect, the action of reducing melanin to lighten its color (action 1), the action of suppressing the activity of the tyrosinase enzyme involved in melanogenesis (action 2) and / or the activation of melanocytes A substance having an action (action 3) of suppressing a factor to be developed has also been developed.
Examples of such substances include vitamin C (L-ascorbic acid) having the above action 1 and derivatives thereof, hydroquinone derivatives such as arbutin having the above action 2, ellagic acid, kojic acid, and the above action 3 And tranexamic acid having However, no satisfactory whitening effect was obtained with any substance.

  In addition, a mixed composition of an extract obtained by extracting koboku (barberry bark), which is used as a herbal medicine with an intestinal and gastric action, with water or an organic solvent and other plant extracts may also have a skin beautifying effect. Although known (Patent Document 1), its mechanism of action has not been sufficiently elucidated, and a sufficient skin-beautifying effect is difficult to obtain with a Koboku extract alone.

JP 2006-328048 A Japanese Patent Laying-Open No. 2005-104873 JP 2005-179226 A

In view of the circumstances as described above, the present inventors have studied to extract honoki with a solvent different from conventional extraction solvents such as water and alcohol in order to obtain a honoki extract with a higher skin beautifying effect. We focused on the extraction method using supercritical carbon dioxide.
So far, it has been known that an extract can be efficiently obtained from a plant using supercritical carbon dioxide (Patent Documents 2 and 3). However, it has not been known that a honoki extract obtained from supercritical carbon dioxide and the extract have an excellent skin beautifying effect.

  An object of this invention is to provide the honoki extract which has the skin beautifying effect superior to the honoki extract by conventional extraction solvents, such as water and alcohol, its manufacturing method, and the skin beautifying composition containing this extract.

  As a result of diligent research, the present inventors have extracted honoki with supercritical carbon dioxide, so that the skin beautifying effect is superior to that of a honoki extract using conventional extraction solvents such as water and alcohol, in particular, tyrosinase and hyaluronidase enzyme activities. As a result, it was found that a honoki extract having an inhibitory action can be obtained, thereby completing the present invention.

  Accordingly, the present invention provides a honoki extract obtained by extracting honoki with supercritical carbon dioxide, a method for producing the same, and a skin beautifying composition containing the extract.

  According to the honoki extract of the present invention extracted with supercritical carbon dioxide, the skin beautifying effect superior to the composition containing the honoki extract with a conventional extraction solvent such as water or alcohol, especially the suppression of the enzyme activity of tyrosinase and hyaluronidase. The skin-beautifying composition which has an effect | action can be provided.

  As the honoki used in the present invention, flowers of magnoliaceae (Magnoliaceae) such as honoki (Magnolia obovata Thunberg), senboku (Magnolia officinalis Rehder et Wilson), onboku (Magnolia officinalis Rehder et Wilson var. Biloba Rehder et Wilson) , Flower ears, pericarps, fruits, stems, leaves, branches, branches and leaves, stems, bark, rhizomes, root barks, roots, seeds, etc., among which the bark of these plants is preferably used.

The bark of the above plant is also called Koboku, and is known as a herbal medicine having an intestinal regulating action and a stomachic action, and can be obtained on the market.
The honoki, such as Amber, used as a raw material may be used as it is, but it is preferable to use a dried and / or pulverized one.

  As used herein, “skin-beautifying effect” refers to preventing and / or improving skin pigmentation such as spots and freckles, and restoring to normal skin color, and preventing and / or improving skin aging. Means the effect. Therefore, the skin beautifying composition of the present invention means a composition having such a skin beautifying effect.

  In the present specification, “skin aging” means skin wrinkles and sagging caused by effects such as aging and ultraviolet rays, and deterioration of skin functions such as moisture retention and elasticity.

  “Tyrosinase” is an enzyme that reacts with tyrosine and dopa, which are precursors of melanin, in melanocytes as substrates, and promotes the production of melanin.

  “Hyaluronidase” is an enzyme that degrades hyaluronic acid present in skin, blood vessels, joints, and the like. By inhibiting the activity of the enzyme, the degradation of hyaluronic acid in vivo can be suppressed, and the amount of hyaluronic acid in the skin can be kept constant. As a result, skin aging can be prevented and / or improved.

“Supercritical” in supercritical carbon dioxide means a state exceeding a critical temperature and pressure (hereinafter referred to as “critical point”) at which a gas and a liquid of a certain substance can coexist.
The critical point of carbon dioxide is a temperature of 31.1 ° C. and a pressure of 7.38 MPa. Thus, supercritical carbon dioxide means fluid carbon dioxide at a temperature above the critical point and under a pressure above the critical point.

The temperature and pressure of supercritical carbon dioxide used for extraction in the present invention can be appropriately set by those skilled in the art, but the temperature is generally 31.1 to 120 ° C., preferably 40 to 100 ° C., more preferably 50 to The temperature is 90 ° C, more preferably 60 to 85 ° C, and the pressure is generally 7.38 to 100 MPa, preferably 10 to 80 MPa, more preferably 15 to 60 MPa, and further preferably 20 to 50 MPa.
Further, the flow rate of supercritical carbon dioxide and the extraction time during extraction are not particularly limited and can be appropriately set by those skilled in the art.

In the extraction with supercritical carbon dioxide in the present invention, a co-solvent (entrainer) may be used for the purpose of improving the extraction efficiency. Examples of entrainers include organic solvents such as methanol, ethanol, isopropyl alcohol, butanol, acetone, hexane, cyclohexane, and toluene, and one or more of these can be used, but from the viewpoint of safety. Ethanol, water or a mixture thereof is preferred.
The amount of entrainer is not particularly limited, but is preferably about 10% by weight or less, more preferably about 5% by weight or less of the amount of supercritical carbon dioxide.

The honoki extract in the present invention can be produced, for example, as follows.
First, a predetermined amount of honoki is placed in a pressurized container (extraction tank) of a supercritical fluid extraction apparatus, and extraction is performed by continuously supplying supercritical carbon dioxide into the container. Next, the supercritical carbon dioxide containing the honoki extract is transported to the separation tank, and the carbon dioxide and the honoki extract are separated by a commonly used method, for example, a method of lowering pressure and / or temperature. As a result, the honoki extract of the present invention is obtained.

  The separation tank may be filled with an adsorbent capable of adsorbing the honoki extract, a solvent or a base capable of dissolving or dispersing the extract, and the like. The separated carbon dioxide can be transported to the liquefaction tank and reused.

  The honoki extract obtained as described above may be subjected to treatments such as concentration, dilution, filtration, deodorization, decolorization, and drying as necessary. In addition, specific components contained in the extract may be isolated and / or concentrated by column purification or the like.

  The honoki extract of the present invention has an action of inhibiting the activity of tyrosinase, which is an enzyme deeply involved in the production of melanin that causes stains and buckwheat, and hyaluronidase, which is an enzyme that degrades hyaluronic acid. Therefore, the honoki extract of the present invention has an excellent skin beautifying effect.

The honoki extract of the present invention may be used as it is, but other ingredients usually used in technical fields such as cosmetics, quasi-drugs, pharmaceuticals or foods are used as long as the skin beautifying effect of the extract is not impaired. You may mix | blend suitably with the honoki extract of invention, and may use it as the skin beautifying composition of this invention.
Examples of the other components include fats and oils (cocoa butter, coconut oil, palm kernel oil, etc.), waxes (beeswax, carnauba wax, etc.), hydrocarbons (solid paraffin, liquid paraffin, petrolatum, etc.), fatty acids (Oleic acid, linoleic acid, isostearic acid, undecylenic acid, etc.), alcohols (ethanol, cetyl alcohol, isostearyl alcohol, etc.), esters (myristyl myristate, glyceryl monostearate, decyl oleate, etc.), surfactants (Sodium lauryl sulfate, sorbitan stearate, stearyldimethylbenzylammonium chloride, etc.), fragrance (synthetic fragrance, natural fragrance, etc.), sweetener (sucrose, stevia, etc.), pH adjuster (sodium bicarbonate, potassium carbonate, etc.), Preservative (sodium benzoate, Rubic acid, etc.), moisturizers (glycerin, collagen, hyaluronic acid, etc.), powders (pigments, dyes, resins, etc.), UV absorbers (e.g. 2-ethylhexyl paramethoxycinnamate), UV scattering agents (titanium oxide, oxidation) Zinc), thickeners (gum arabic, methylcellulose, etc.), antioxidants (vitamin C, vitamin E, etc.), chelating agents (disodium edetate, sodium citrate, sodium metaphosphate, etc.), lubricants (stearin) Magnesium oxide, talc, polyethylene glycol, etc.), disintegrants (starch, crystalline cellulose, etc.) and the like.

  In addition to the honoki extract of the present invention, the skin beautifying composition of the present invention may further contain other whitening agents or other herbal extracts having a whitening effect. Such a whitening agent is not particularly limited as long as it has a melanin production inhibitory action, and examples thereof include hydroquinone derivatives such as vitamin C (L-ascorbic acid) and derivatives thereof, and arbutin (hydroquinone β-D-glucose). , Ellagic acid, kojic acid, tranexamic acid and the like.

  The skin beautifying composition of the present invention can be provided as a composition used for skin whitening or a composition used for preventing and / or improving skin aging.

  The honoki extract and skin-beautifying composition of the present invention can be incorporated into any of cosmetics, quasi-drugs, pharmaceuticals or foods (including foods for specified health use and nutritional functional foods). , Lotion, cream, emulsion, foundation, essence (extract), pack, mask, lipstick, dusting, cleansing lotion, shampoo, rinse, conditioner, soap, body shampoo, bath preparation, sunscreen lotion, ointment, poultice, Examples thereof include powders, tablets, emulsions, troches, liquids for internal use, syrups, gels, aerosols, beverages and confectionery.

  The content of the honoki extract of the present invention in the cosmetics, quasi-drugs, pharmaceuticals or foods (including foods for specified health use and nutritional functional foods) is not particularly limited, and should be appropriately set according to each form. Can do. As an example, the content is 0.0001 to 50 honoki extract as a dry weight with respect to the total weight of cosmetics, quasi drugs, pharmaceuticals or foods (including foods for specified health use and nutritional functional foods). % By weight, preferably 0.0005 to 40% by weight, more preferably 0.001 to 30% by weight.

  When the skin beautifying composition containing the honoki extract of the present invention is a skin external preparation, it is usually sufficient to add 0.001 to 10% by weight of the honoki extract as a dry weight with respect to the total weight of the skin external preparation. However, if it is less than 0.001% by weight, a sufficient skin-beautifying effect cannot be expected. Conversely, even if it exceeds 10% by weight, an enhancement of the skin-beautifying effect corresponding to the excess cannot be expected, which is uneconomical.

  In addition, the amount of intake when taking the honoki extract or skin-beautifying composition of the present invention varies depending on the administration form, age, body weight, etc., but 0.01-10 g / day, preferably 0, as the dry weight of the honoki extract. .1-3 g / day.

  The present invention will be described more specifically with reference to examples. However, the technical scope of the present invention is not limited by these examples.

1. Preparation of honoki (Koboku) extract (Examples 1-4)
45 g of pulverized product of dried Koboku (produced in Japan, Samsung Pharmaceutical Co., Ltd.) is put into an extraction tank (capacity 200 ml; manufactured by ITEC Co., Ltd.) of a supercritical fluid extraction device, and the temperature and pressure superfluous shown in Table 1 are added to this. Critical carbon dioxide was supplied at a flow rate of 2.7 kg / hour and extracted for 2 hours. In the separation tank of the apparatus, the extract was separated from carbon dioxide and recovered by separating under reduced pressure. Table 1 shows the weight of each of the obtained extract.

(Comparative Example 1)
1000 ml of water was added to 100 g of the pulverized dried product of Koboku and extracted at 55 ° C. for 1 hour. Next, the extract was filtered, and the filtrate was concentrated to 30 ml under reduced pressure, and then lyophilized to obtain 8.7 g of a water extract of amber.
(Comparative Example 2)
1000 g of 50% aqueous ethanol was added to 100 g of the pulverized dried product, and the mixture was extracted at 55 ° C. for 1 hour. Next, the extract was filtered, and the filtrate was concentrated to 30 ml under reduced pressure, and then lyophilized to obtain 8.8 g of an amber ethanol extract.

2. Test for inhibiting tyrosinase activity In order to examine the whitening effect of each extract obtained in Examples 1 to 4 and Comparative Examples 1 and 2, a test for inhibiting tyrosinase activity, which is one of melanin production inhibitory actions Was performed as follows.
(1) Preparation of sample solution Each of the above extract is dissolved in dimethyl sulfoxide (DMSO) to prepare sample solutions at four levels (125, 250, 500 and 1000 mg / ml). The sample solution was added to the reaction system so as to be diluted 100 times.
(2) Preparation of Tyrosinase Enzyme Solution Tyrosinase (derived from mushrooms; Sigma) was dissolved in 1/15 M phosphate buffer (pH 6.8) to prepare a tyrosinase enzyme solution (300 units / ml).
(3) Preparation of substrate solution L-tyrosine (Sigma) or L-dopa (Sigma) was dissolved in 1/15 M phosphate buffer (pH 6.8) to give a 0.03% substrate solution (0.3 mg). / Ml) was prepared.

(4) Preparation of test reaction solution and test blank solution To a 96-well plate (Microplate; ASONE Co., Ltd.), 3 μl of each sample solution was added per well, and then 1/15 M phosphate buffer (pH 6. 80 μl of 8) and 150 μl of substrate solution were added. As a blank control, 150 μl of sterilized water was added to the wells to which each sample solution was added instead of the substrate solution. The plates were then preincubated for 10 minutes at 37 ° C.
And 70 microliters of tyrosinase enzyme solutions were added to each well, and it incubated for 30 minutes at 37 degreeC, and obtained the test reaction liquid and the test blank liquid.
(5) Preparation of control reaction solution and control blank solution In the above step (4), 3 μl of DMSO was added to each well of a 96-well plate in place of each sample solution, and the subsequent operations were the same as in step (4) above. Similarly, a control reaction solution and a control blank solution were obtained.

(6) Measurement of absorbance and calculation of inhibition rate of tyrosinase activity After completion of the incubation in the above steps (4) and (5), the absorbance (490 nm) of the reaction solution in each well was measured with a microplate reader (Model 680; Bio-Rad). ). In addition, the light absorbency measured originates in the dopachrome which is a reaction product.
Next, the inhibition rate of tyrosinase activity was calculated from the obtained measured values using the following formula.
Tyrosinase activity inhibition rate (%) = [1− (A−B) / (C−D)] × 100
(A: measured value of test reaction solution, B: measured value of test blank solution, C: measured value of control reaction solution,
D: Measured value of control blank solution)

  Moreover, arbutin (β type) was selected as a positive control. Arbutin (Tokyo Kasei Kogyo Co., Ltd.) was replaced with the extract of arbutin in the above step (1), and a sample solution of arbutin was prepared in the same manner. It carried out like (5).

The inhibition rates calculated above are shown in Table 2. Table 3 shows the effective concentration (IC ₅₀ ) showing 50% inhibition rate of tyrosinase activity.

As shown in Table 2, the extract of Examples 1 to 4 has a significantly higher inhibition rate of tyrosinase activity than the extract of Comparative Examples 1 and 2 and the positive control (arbutin) at each corresponding concentration. Indicated.
Further, as shown in Table 3, the IC ₅₀ of the extract of Examples 1 to 4 showed a significantly lower value than the IC _{50 of the} extract of Comparative Examples 1 and 2.
From these results, it was confirmed that the amber extract extracted with supercritical carbon dioxide has a superior tyrosinase activity inhibitory action than the conventional amber extract with water or alcohol.

3. Hyaluronidase activity inhibitory action test In order to examine the prevention and / or improvement effect of skin aging of each extract obtained in Examples 1 to 4 and Comparative Examples 1 and 2, the test for hyaluronidase activity inhibitory action was conducted. We went as follows.
(1) Preparation of sample solution Each above-mentioned extract is dissolved in DMSO to prepare sample solutions of 4 levels (31.3, 62.5, 125 and 250 mg / ml). The sample solution was added to the reaction system so as to be diluted 100 times.
(2) Preparation of Hyaluronidase Enzyme Solution Hyaluronidase (derived from bovine testis; Sigma) was dissolved in 100 mM acetate buffer (pH 4.0) to prepare a hyaluronidase enzyme solution (400 units / ml).
(3) Preparation of enzyme activator Compound 48/80 (Sigma) was dissolved in 100 mM acetate buffer (pH 4.0) to prepare an enzyme activator (0.1 mg / ml).
(4) Preparation of substrate solution Potassium hyaluronate (derived from human umbilical cord; Wako Pure Chemical Industries, Ltd.) was dissolved in 100 mM acetate buffer (pH 4.0) to prepare a 0.4 mg / ml substrate solution.

(5) Preparation of test reaction solution and test blank solution To a 96 deep well plate (As One Co., Ltd.), 1 μl of each sample solution was added per well, and then 29 μl of hyaluronidase enzyme solution was added to each well. As a blank control, 29 μl of 100 mM acetate buffer (pH 4.0) was added to the well to which each sample solution was added instead of the enzyme solution. The plates were then preincubated for 20 minutes at 37 ° C. Next, 20 μl of enzyme activator was added per well and incubated at 37 ° C. for 20 minutes, after which 50 μl of substrate solution was added and incubated at 37 ° C. for 80 minutes. Then, 20 μl of 0.4N sodium hydroxide solution was added and ice-cooled to stop the reaction, 20 μl of 5% boric acid solution (pH 9.1) was added, heated in a boiling bath for 5 minutes, and then ice-cooled. Thereafter, 10 g of 1% p-DAB (p-dimethylaminobenzaldehyde) solution (Wako Pure Chemical Industries, Ltd.) dissolved in 12.5 ml of 10N hydrochloric acid and 87.5 ml of acetic acid was diluted 10-fold with acetic acid immediately before use. ) Was added and incubated at 37 ° C. for 20 minutes for color development to obtain a test reaction solution and a test blank solution.

(6) Preparation of control reaction solution and control blank solution In the above step (5), 1 μl of DMSO was added per well of a 96 deep well plate in place of each sample solution, and the subsequent operation was performed in the above step (5). In the same manner as described above, a control reaction solution and a control blank solution were obtained.

(7) Measurement of absorbance and calculation of inhibition rate of hyaluronidase activity After completion of the incubation in the above steps (5) and (6), 300 μl of the reaction solution per well was transferred to a 96-well plate (As One Co., Ltd.) The absorbance (585 nm) of the reaction solution was measured with a microplate reader (Model 680; manufactured by Bio-Rad). In addition, the light absorbency measured originates in the increase in the reducing power of the tetrasaccharide which uses N-acetylhexosamine produced | generated by a hydrolysis of hyaluronic acid as a reducing terminal.
Next, the inhibition rate of hyaluronidase activity was calculated from each measured value using the following formula.
Hyaluronidase activity inhibition rate (%) = [1− (A−B) / (C−D)] × 100
(A: measured value of test reaction solution, B: measured value of test blank solution, C: measured value of control reaction solution, D: measured value of control blank solution)
Moreover, sodium cromoglycate (as described in the Japanese Pharmacopoeia) was selected as a positive control. Calculation of the inhibition rate of hyaluronidase activity of cromoglycate sodium was carried out by preparing a sample solution of cromoglycate sodium in the same manner using sodium cromoglycate instead of the kumoku extract of the above step (1). Performed in the same manner as in (7).
The inhibition rate calculated above is shown in Table 4. In addition, Table 5 shows the effective concentration (IC ₅₀ ) showing a 50% inhibition rate of hyaluronidase activity.

As shown in Table 4, the extract of Examples 1 to 4 showed a higher inhibition rate of hyaluronidase activity than the extract of Comparative Examples 1 and 2 at each corresponding concentration.
Further, as shown in Table 5, the IC ₅₀ of the extract of Examples 1-4 was significantly lower than the IC _{50 of the} extract of Comparative Examples 1 and 2.
From these results, it was confirmed that the amber extract extracted with supercritical carbon dioxide has a hyaluronidase activity inhibitory action superior to that of a conventional amber extract with water or alcohol.

4). Formulation Examples of the skin beautifying composition of the present invention are shown below. The honoki extract is the same as the above 1. The product obtained by extraction with supercritical carbon dioxide described in 1. was used. Further, the blending ratio of each component is shown by weight%.
(Prescription Example 1)
Cream A cosmetic (cream), which is an external preparation for skin of the present invention, was produced. That is, each component of (A) was mixed and heated to 80 ° C. On the other hand, the components (B) were mixed and heated to 80 ° C. To the mixture of (A), the mixture of (B) was gradually added and emulsified with stirring, and then cooled to 35 ° C. to obtain a cream.
(A)
Squalane 15.00 (wt%)
Octyldodecyl myristate 4.00
Hydrogenated soybean phospholipid 0.20
Butyl alcohol 2.40
Hardened oil 1.50
Stearic acid 1.50
Lipophilic glyceryl monostearate 1.50
Polyglyceryl monostearate 0.50
Behenyl alcohol 0.80
Polyglyceryl monomyristate 0.70
White beeswax 0.30
d-δ-tocopherol 0.05
Methylparaben 0.30
Honoki extract 1.00
(B)
C10-30 alkyl-modified carboxyvinyl polymer 0.20
Carboxyvinyl polymer 0.10
1,3-butanediol 18.00
Sodium hydroxide 0.10
Purified water 51.85
(Total 100.00)

(Prescription example 2)
Cosmetic lotion A cosmetic preparation (lotion), which is an external preparation for skin of the present invention, was produced. That is, the components (A) are mixed, heated to 80 ° C. to make the mixture uniform, and then cooled. To this, each component of (B) was sequentially added at 35 ° C., mixed and homogenized to obtain a lotion.
(A)
Squalane 0.40 (wt%)
Polyglyceryl monolaurate 0.10
Long chain α-hydroxy fatty acid 0.01
Glycerin 3.00
1,3-butylene glycol 8.00
Honoki extract 1.00
(B)
Phenoxyethanol 0.20
1,3-butylene glycol 10.00
Allantoin 0.10
Citric acid 0.01
Diethylenetriaminepentaacetate solution 0.05
Succinyl collagen solution 0.01
Purified water 77.12
(Total 100.00)

  The honoki extract of the present invention extracted with supercritical carbon dioxide has excellent tyrosinase activity inhibitory action and hyaluronidase activity inhibitory action, and therefore has an excellent skin beautifying effect, that is, whitening effect and skin aging prevention and / or improvement effect. And can be used as an active ingredient in cosmetics, quasi drugs, pharmaceuticals or foods.

Claims (9)

  A honoki extract obtained by extracting honoki with supercritical carbon dioxide.   The honoki extract according to claim 1, wherein the honoki is Koboku.   A beautifying skin composition comprising the honoki extract according to claim 1 or 2.   The skin beautifying composition according to claim 3, wherein the composition is a skin whitening composition.   The skin-beautifying composition according to claim 3, wherein the composition is a composition for preventing and / or improving skin aging.   The skin-beautifying composition according to any one of claims 3 to 5, wherein the composition is a cosmetic, a quasi-drug, a pharmaceutical, or a food.   The skin-beautifying composition according to claim 6, wherein the cosmetic is in the form of lotion, cream, emulsion, foundation, essence, pack, mask, lipstick, dusting or cleansing lotion.   The method for producing a honoki extract according to claim 1, wherein the honoki is extracted with supercritical carbon dioxide to obtain a honoki extract.   The production method according to claim 8, wherein the honoki is Koboku.