JP2010532986A - 改良された微生物の検出 - Google Patents

改良された微生物の検出 Download PDF

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Publication number
JP2010532986A
JP2010532986A JP2010515596A JP2010515596A JP2010532986A JP 2010532986 A JP2010532986 A JP 2010532986A JP 2010515596 A JP2010515596 A JP 2010515596A JP 2010515596 A JP2010515596 A JP 2010515596A JP 2010532986 A JP2010532986 A JP 2010532986A
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JP
Japan
Prior art keywords
nad
nucleic acid
sample
dependent ligase
acid molecule
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Pending
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JP2010515596A
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English (en)
Japanese (ja)
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JP2010532986A5 (https=
Inventor
クリストファー， ジョン スタンリー，
スチュアート ウィルソン，
Original Assignee
マイクロセンス メドテック リミテッド
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Priority claimed from GB0713255A external-priority patent/GB0713255D0/en
Priority claimed from GB0719785A external-priority patent/GB0719785D0/en
Priority claimed from GB0802857A external-priority patent/GB0802857D0/en
Priority claimed from GB0807054A external-priority patent/GB0807054D0/en
Application filed by マイクロセンス メドテック リミテッド filed Critical マイクロセンス メドテック リミテッド
Publication of JP2010532986A publication Critical patent/JP2010532986A/ja
Publication of JP2010532986A5 publication Critical patent/JP2010532986A5/ja
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/18—Testing for antimicrobial activity of a material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/25—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving enzymes not classifiable in groups C12Q1/26 - C12Q1/66
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/9015—Ligases (6)

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Toxicology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2010515596A 2007-07-09 2008-07-09 改良された微生物の検出 Pending JP2010532986A (ja)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
GB0713255A GB0713255D0 (en) 2007-07-09 2007-07-09 Improved methods and kits for detecting an enzyme capable of modifying a nucleic acid
GB0713972A GB0713972D0 (en) 2007-07-09 2007-07-18 Improved methods and kits for detecting an enzyme capable of modifying a nucleic acid
GB0719785A GB0719785D0 (en) 2007-07-09 2007-10-10 Improved detection of micro-organisms
GB0802857A GB0802857D0 (en) 2008-02-15 2008-02-15 Improved bacterial detection methods and kits
GB0807054A GB0807054D0 (en) 2008-04-17 2008-04-17 Improved detection of micro-organisms
PCT/GB2008/002354 WO2009007719A2 (en) 2007-07-09 2008-07-09 Detection of micro-organisms based on their nad-dependent dna ligase activity

Publications (2)

Publication Number Publication Date
JP2010532986A true JP2010532986A (ja) 2010-10-21
JP2010532986A5 JP2010532986A5 (https=) 2011-09-01

Family

ID=39790901

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2010515596A Pending JP2010532986A (ja) 2007-07-09 2008-07-09 改良された微生物の検出

Country Status (8)

Country Link
US (1) US20100291564A1 (https=)
EP (1) EP2179052B1 (https=)
JP (1) JP2010532986A (https=)
CN (1) CN101849019A (https=)
AU (1) AU2008273946A1 (https=)
CA (1) CA2692800A1 (https=)
MX (1) MX2010000031A (https=)
WO (1) WO2009007719A2 (https=)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0906643D0 (en) 2009-04-17 2009-06-03 Wilson Stuart M Detection of bacteria and fungi
KR102068136B1 (ko) 2010-04-16 2020-01-20 모멘텀 바이오사이언스, 리미티드 비-정제된 샘플에서 세포 생존력을 결정하는데에 유용한 효소 활성을 측정하기 위한 방법
KR20150005511A (ko) 2012-01-05 2015-01-14 제우스 사이언티픽, 아이엔씨. 개선된 dna 중합효소 활성 분석 및 생육성 미생물의 검출을 가능하게 하는 방법
JP2015513921A (ja) * 2012-04-12 2015-05-18 ゼウス サイエンティフィック、インク． 任意のポリメラーゼ伸長活性の高感度定量的測定と生細胞存在の決定とに有用なポリメラーゼ活性測定方法
GB2528647A (en) * 2014-07-10 2016-02-03 Momentum Bioscience Ltd Detecting viable microorganisms
CN108449942A (zh) * 2015-05-01 2018-08-24 微生物发现集团有限责任公司 核酸检测和定量方法以及组合物
US12157690B2 (en) 2015-12-09 2024-12-03 Microbial Discovery Group Llc Cold temperature-resistant microbials and methods of their use
CA3016203C (en) 2016-02-29 2024-06-11 Microbial Discovery Group, Llc Direct-fed microbials
WO2017182775A1 (en) * 2016-04-18 2017-10-26 Momentum Bioscience Limited Microorganism detection involving filtration
US20200354767A1 (en) * 2017-11-16 2020-11-12 The Regents Of The University Of Colorado, A Body Corporate Methods to measure functional heterogeneity among single cells
EP3749775B1 (en) * 2018-02-07 2023-06-07 Mycometer A/S Improved method for measurement of microbial biomass
GB201805479D0 (en) 2018-04-03 2018-05-16 Momentum Bioscience Ltd Microorganism separation and detection
WO2020072578A1 (en) 2018-10-02 2020-04-09 Microbial Discovery Group, Llc Microbials for animals
CN111118181B (zh) * 2018-10-31 2023-05-12 华东师范大学 一种细菌的检测方法
CN114929027A (zh) 2019-08-22 2022-08-19 微生物发现集团有限责任公司 用微生物菌株和抗生素的抑制方法
GB201914538D0 (en) 2019-10-08 2019-11-20 Momentum Bioscience Ltd Microorganism capture from antimicrobial-containing solution
BR112023002974A2 (pt) 2020-08-21 2023-04-04 Microbial Discovery Group Llc Cepas microbianas e antibióticos
US12514885B2 (en) 2020-08-21 2026-01-06 Microbial Discovery Group, Llc Microbials and antibiotics
GB202405229D0 (en) 2024-04-12 2024-05-29 Momentum Bioscience Ltd Microorganism enumeration

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5976806A (en) * 1997-06-25 1999-11-02 Pioneer Hi-Bred International, Inc. DNA ligase assay
WO2005093089A1 (en) * 2004-03-22 2005-10-06 Goldschmidt Gesellschaft Mit Beschränkter Haftung Method and test-kit for the detection and quantification of organisms

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4437975A (en) 1977-07-20 1984-03-20 Mobil Oil Corporation Manufacture of lube base stock oil
US4931390A (en) * 1986-04-16 1990-06-05 Public Health Research Institute Of The City Of New York, Inc. Expression of the cloned lysostaphin gene
CA1340843C (en) 1987-07-31 1999-12-07 J. Lawrence Burg Selective amplification of target polynucleotide sequences
CA2005589C (en) 1988-12-16 2001-02-06 Akzo Nobel Nv Self-sustained, sequence replication system
DE4033752A1 (de) * 1990-10-24 1992-04-30 Reissbrodt Rolf Dr Sc Nat Verfahren zur herstellung von lysostaphin
JP3267576B2 (ja) 1998-11-09 2002-03-18 三光純薬株式会社 プローブ高分子の形成方法
MXPA02003885A (es) * 1999-10-19 2005-06-03 Bharat Biotech International L Expresion de lisostafina madura recombinante.
US6558907B2 (en) * 2001-05-16 2003-05-06 Corning Incorporated Methods and compositions for arraying nucleic acids onto a solid support
WO2004067726A2 (en) 2003-01-29 2004-08-12 Keck Graduate Institute Isothermal reactions for the amplification of oligonucleotides
JP2006526399A (ja) * 2003-06-02 2006-11-24 チェック−ポインツ ホールディング ベー．フェー． 食物サンプル中の微生物を高速に検出する方法
GB0324650D0 (en) * 2003-10-22 2003-11-26 Acolyte Biomedica Ltd Using nucleic acids for clinical microbiology testing
US20070009944A1 (en) * 2005-07-01 2007-01-11 University Of East Anglia Assay for nucleic acid ligase and nucleic acid nuclease

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5976806A (en) * 1997-06-25 1999-11-02 Pioneer Hi-Bred International, Inc. DNA ligase assay
WO2005093089A1 (en) * 2004-03-22 2005-10-06 Goldschmidt Gesellschaft Mit Beschränkter Haftung Method and test-kit for the detection and quantification of organisms

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
JPN5010012013; ＬＩＵ　ＬＩＮＧＦＥＮＧ: ＴＨＥ　ＡＮＡＬＹＳＴ Ｖ１３０　Ｎ３, 200503, Ｐ３５０-３５７, ＴＨＥ　ＲＯＹＡＬ　ＳＯＣＩＥＴＹ　ＯＦ　ＣＨＥＭＩＳＴＲＹ *
JPN6013020891; Ａｎａｌ．　Ｂｉｏｃｈｅｍ．，　３５８（２００６）　ｐ．９０-９８ *
JPN6013020892; Ａｎａｌ．　Ｂｉｏｃｈｅｍ．，　３０９（２００２）　ｐ．２３２-２４０ *

Also Published As

Publication number Publication date
US20100291564A1 (en) 2010-11-18
CA2692800A1 (en) 2009-01-15
WO2009007719A2 (en) 2009-01-15
EP2179052A2 (en) 2010-04-28
CN101849019A (zh) 2010-09-29
MX2010000031A (es) 2010-04-21
WO2009007719A3 (en) 2009-04-09
EP2179052B1 (en) 2012-11-14
AU2008273946A1 (en) 2009-01-15

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