JP2010529855A - 間葉系フィーダー細胞由来のパラ分泌シグナルならびにそれを用いる肝前駆体の増加および分化の調整 - Google Patents

間葉系フィーダー細胞由来のパラ分泌シグナルならびにそれを用いる肝前駆体の増加および分化の調整 Download PDF

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JP2010529855A
JP2010529855A JP2010512194A JP2010512194A JP2010529855A JP 2010529855 A JP2010529855 A JP 2010529855A JP 2010512194 A JP2010512194 A JP 2010512194A JP 2010512194 A JP2010512194 A JP 2010512194A JP 2010529855 A JP2010529855 A JP 2010529855A
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cells
collagen
layer
liver
container
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リード,ローラ,エム.
マクルランド,ランドール,イー.
ウロニス,ジョシュア
ヤオ,シン−レイ
ウォーティア,エリアーヌ
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ユニバーシティ オブ ノース カロライナ アット チャペル ヒル
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0652Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662Stem cells
    • C12N5/0667Adipose-derived stem cells [ADSC]; Adipose stromal stem cells
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    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/067Hepatocytes
    • C12N5/0672Stem cells; Progenitor cells; Precursor cells; Oval cells
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    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
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    • C12N2502/00Coculture with; Conditioned medium produced by
    • C12N2502/13Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
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    • C12N2533/00Supports or coatings for cell culture, characterised by material
    • C12N2533/50Proteins
    • C12N2533/52Fibronectin; Laminin
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    • C12N2533/00Supports or coatings for cell culture, characterised by material
    • C12N2533/50Proteins
    • C12N2533/54Collagen; Gelatin
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00Supports or coatings for cell culture, characterised by material
    • C12N2533/70Polysaccharides
    • C12N2533/80Hyaluronan

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
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  • Biomedical Technology (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
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  • Biochemistry (AREA)
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  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Rheumatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
JP2010512194A 2007-06-15 2008-06-13 間葉系フィーダー細胞由来のパラ分泌シグナルならびにそれを用いる肝前駆体の増加および分化の調整 Pending JP2010529855A (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US94443507P 2007-06-15 2007-06-15
PCT/US2008/007397 WO2008156667A1 (en) 2007-06-15 2008-06-13 Paracrine signals from mesenchymal feeder cells and regulating expansion and differentiation of hepatic progenitors using same

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JP2010529855A true JP2010529855A (ja) 2010-09-02

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JP2010512194A Pending JP2010529855A (ja) 2007-06-15 2008-06-13 間葉系フィーダー細胞由来のパラ分泌シグナルならびにそれを用いる肝前駆体の増加および分化の調整

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US (2) US20080318316A1 (https=)
EP (1) EP2171040A4 (https=)
JP (1) JP2010529855A (https=)
KR (1) KR20100094970A (https=)
CN (1) CN101868533A (https=)
AU (1) AU2008266992A1 (https=)
BR (1) BRPI0813353A2 (https=)
CA (1) CA2727535A1 (https=)
IL (1) IL202738A0 (https=)
IN (1) IN2010KN00157A (https=)
MX (1) MX2009013819A (https=)
TW (1) TW200916581A (https=)
WO (1) WO2008156667A1 (https=)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPWO2016010082A1 (ja) * 2014-07-16 2017-06-22 国立大学法人大阪大学 ラミニンフラグメントの細胞培養基質活性増強方法
JP2020505076A (ja) * 2017-02-01 2020-02-20 フェニックスソングス バイオロジカルズ,インコーポレイテッド 肝細胞の共培養を含む組成物および方法
WO2020075534A1 (ja) * 2018-10-11 2020-04-16 一丸ファルコス株式会社 神経幹細胞増殖用培地

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070269412A1 (en) * 2003-12-02 2007-11-22 Celavie Biosciences, Llc Pluripotent cells
US20100209399A1 (en) * 2009-02-13 2010-08-19 Celavie Biosciences, Llc Brain-derived stem cells for repair of musculoskeletal system in vertebrate subjects
CN105456292B (zh) * 2009-10-30 2019-03-26 北卡罗来纳大学教堂山分校 来自肝外胆管树的专能干细胞及其分离方法
AU2011247984B2 (en) * 2010-05-07 2015-04-30 University Of North Carolina At Chapel Hill Method of engrafting cells from solid tissues
ITMI20120896A1 (it) * 2012-05-23 2013-11-24 Bongulielmi Reto Condroitina per uso in medicina
JP6435267B2 (ja) * 2012-11-08 2018-12-05 インジェネロン インコーポレイテッド 再生細胞の培養、保存、及び投与のための培地
US9533013B2 (en) 2013-03-13 2017-01-03 University Of North Carolina At Chapel Hill Method of treating pancreatic and liver conditions by endoscopic-mediated (or laparoscopic-mediated) transplantation of stem cells into/onto bile duct walls of particular regions of the biliary tree
US11066649B2 (en) 2014-03-19 2021-07-20 Institut National De La Sante Et De La Recherche Medicale (Inserm) Method for inducing human cholangiocyte differentiation
WO2016200340A1 (en) 2015-06-12 2016-12-15 Agency For Science, Technology And Research Derivation of hepatic stem cells and mature liver cell types and uses thereof
US20170369849A1 (en) * 2016-05-11 2017-12-28 The University Of North Carolina At Chapel Hill Compositions and methods for bioengineered tissues
EP3684386A4 (en) * 2017-09-22 2021-06-30 Lifenet Health PLATE HEPATOCYTES AND THE PREPARATION AND USES OF THEM

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007059501A2 (en) * 2005-11-16 2007-05-24 University Of North Carolina At Chapel Hill Extracellular matrix components for expansion or differentiation of hepatic progenitors
US20070134789A1 (en) * 1991-08-07 2007-06-14 Albert Einstein College Of Medicine Of Yeshiva University Proliferation of hepatocyte precursors

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6069005A (en) * 1991-08-07 2000-05-30 Albert Einstein College Of Medicine Of Yeshwa University Hapatoblasts and method of isolating same
AU779706B2 (en) * 1999-01-19 2005-02-10 University Of North Carolina At Chapel Hill, The Human liver progenitors
US7456017B2 (en) * 1999-10-01 2008-11-25 University Of North Carolina At Chapel Hill Processes for clonal growth of hepatic progenitor cells
HUE032238T2 (en) * 2002-03-15 2017-09-28 Univ North Carolina Chapel Hill Primitive and proximal liver stem cells
CA2689241A1 (en) * 2006-06-28 2008-01-03 Vesta Therapeutics, Inc. Matrix and method for isolation of hepatic progenitor cells
WO2008109659A2 (en) * 2007-03-06 2008-09-12 University Of North Carolina At Chapel Hill Complexes of hyaluronans, other matrix components, hormones and growth factors for maintenance, expansion and/or differentiation of cells

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070134789A1 (en) * 1991-08-07 2007-06-14 Albert Einstein College Of Medicine Of Yeshiva University Proliferation of hepatocyte precursors
WO2007059501A2 (en) * 2005-11-16 2007-05-24 University Of North Carolina At Chapel Hill Extracellular matrix components for expansion or differentiation of hepatic progenitors

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
JPN5002024165; BRILL S: PROCEEDINGS OF THE SOCIETY FOR EXPERIMENTAL BIOLOGY AND MEDICINE V204 N3, 19931201, P261-269 *

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPWO2016010082A1 (ja) * 2014-07-16 2017-06-22 国立大学法人大阪大学 ラミニンフラグメントの細胞培養基質活性増強方法
JP2020505076A (ja) * 2017-02-01 2020-02-20 フェニックスソングス バイオロジカルズ,インコーポレイテッド 肝細胞の共培養を含む組成物および方法
WO2020075534A1 (ja) * 2018-10-11 2020-04-16 一丸ファルコス株式会社 神経幹細胞増殖用培地
JPWO2020075534A1 (ja) * 2018-10-11 2021-09-02 一丸ファルコス株式会社 神経幹細胞増殖用培地
JP7322053B2 (ja) 2018-10-11 2023-08-07 一丸ファルコス株式会社 神経幹細胞増殖用培地

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MX2009013819A (es) 2010-05-17
EP2171040A1 (en) 2010-04-07
US20080318316A1 (en) 2008-12-25
CN101868533A (zh) 2010-10-20
KR20100094970A (ko) 2010-08-27
TW200916581A (en) 2009-04-16
US8404483B2 (en) 2013-03-26
IL202738A0 (en) 2011-08-01
BRPI0813353A2 (pt) 2014-10-14
AU2008266992A1 (en) 2008-12-24
IN2010KN00157A (https=) 2015-08-28
WO2008156667A1 (en) 2008-12-24
EP2171040A4 (en) 2010-08-04
US20110065188A1 (en) 2011-03-17
CA2727535A1 (en) 2008-12-24

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