JP2010511597A5 - - Google Patents
Download PDFInfo
- Publication number
- JP2010511597A5 JP2010511597A5 JP2009524035A JP2009524035A JP2010511597A5 JP 2010511597 A5 JP2010511597 A5 JP 2010511597A5 JP 2009524035 A JP2009524035 A JP 2009524035A JP 2009524035 A JP2009524035 A JP 2009524035A JP 2010511597 A5 JP2010511597 A5 JP 2010511597A5
- Authority
- JP
- Japan
- Prior art keywords
- usa
- gene
- cells
- histone
- vpa
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 210000004027 cells Anatomy 0.000 description 5
- 210000003483 Chromatin Anatomy 0.000 description 2
- 108010077544 Chromatin Proteins 0.000 description 2
- NIJJYAXOARWZEE-UHFFFAOYSA-N Depacane Chemical compound CCCC(C(O)=O)CCC NIJJYAXOARWZEE-UHFFFAOYSA-N 0.000 description 2
- 206010006187 Breast cancer Diseases 0.000 description 1
- QTANTQQOYSUMLC-UHFFFAOYSA-O Ethidium cation Chemical compound C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 QTANTQQOYSUMLC-UHFFFAOYSA-O 0.000 description 1
- 238000006640 acetylation reaction Methods 0.000 description 1
- 239000011543 agarose gel Substances 0.000 description 1
- 102000004965 antibodies Human genes 0.000 description 1
- 108090001123 antibodies Proteins 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 230000001413 cellular Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 239000003276 histone deacetylase inhibitor Substances 0.000 description 1
Description
ヒトの乳癌細胞株、ＭＣＦ−７細胞およびＨＭＣ−１細胞を用いて、１００ｎＭ ＴＳＡ（Ｓｉｇｍａ，ＵＳＡ）存在下２４時間または４ｍＭ ＶＰＡ（Ｓｉｇｍａ，ＵＳＡ）存在下４８時間、それぞれ培養した後、メーカー（Ｕｐｓｔａｔｅ Ｉｎｃ．，ＵＳＡ）の使用説明書に従って、細胞からクロマチンを抽出し抗アセチル化ヒストンＨ３抗体（Ｕｐｓｔａｔｅ Ｉｎｃ．，ＵＳＡ）を用いてクロマチンの免疫沈降を行った（Ａｓｓａｍ Ｅｌ−Ｏｓｔａ ｅｔ ａｌ．，（２００２）ＭＯＬＥＣＵＬＡＲ ＡＮＤ ＣＥＬＬＵＬＡＲ ＢＩＯＬＯＧＹ ２２（６）ｐ１８４４−１８５７を参照のこと）。アセチル化ヒストンと結合している遺伝子を、Ｂ２Ｍ遺伝子特異的プライマー（ＧＡＡＡＡＣＧＧＧＡＡＡＧＴＣＣＣＴＣＴ；配列番号１、およびＡＧＡＴＣＣＡＧＣＣＣＴＧＧＡＣＴＡＧＣ；配列番号２）を用いてＰＣＲにて増幅し、１％アガロースゲルで電気泳動後、臭化エチジウムの存在下、トランスイルミネータで検出した。この結果、いずれの癌細胞においてもＢ２Ｍ遺伝子領域のヒストンは脱アセチル化していることが示された。ＴＳＡやＶＰＡのようなＨＤＡＣ阻害剤の作用によってヒストンアセチル化が促進されると、アセチル化ヒストンとともにＢ２Ｍ遺伝子が検出された（図１）。
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US87322306P | 2006-12-06 | 2006-12-06 | |
| US60/873,223 | 2006-12-06 | ||
| PCT/JP2007/074067 WO2008069349A1 (en) | 2006-12-06 | 2007-12-06 | Potentiation of cellular immunity using histone deacetylase (hdac) inhibitors |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2010511597A JP2010511597A (ja) | 2010-04-15 |
| JP2010511597A5 true JP2010511597A5 (ja) | 2010-06-03 |
| JP4887427B2 JP4887427B2 (ja) | 2012-02-29 |
Family
ID=39124589
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2009524035A Expired - Fee Related JP4887427B2 (ja) | 2006-12-06 | 2007-12-06 | ヒストン脱アセチル化酵素阻害活性を有する物質を用いた細胞性免疫増強剤 |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US20100093862A1 (ja) |
| EP (1) | EP2091525A1 (ja) |
| JP (1) | JP4887427B2 (ja) |
| CN (1) | CN101626763A (ja) |
| AU (1) | AU2007328872A1 (ja) |
| CA (1) | CA2671649A1 (ja) |
| WO (1) | WO2008069349A1 (ja) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2014505076A (ja) * | 2011-01-27 | 2014-02-27 | ザ トラスティーズ オブ プリンストン ユニバーシティ | 抗ウイルス剤としてのｍＴＯＲキナーゼの阻害剤 |
| US9821054B2 (en) * | 2011-03-11 | 2017-11-21 | Turnstone Limited Partnership | Method of vaccination comprising a histone deacetylase inhibitor |
| US10335482B2 (en) | 2012-06-06 | 2019-07-02 | Bionor Immuno As | Method of inducing an anti-HIV-1 immune response comprising administering a C5/TM-GP41 peptide dimer |
| WO2014082114A1 (en) | 2012-11-30 | 2014-06-05 | Commonwealth Scientific And Industrial Research Organisation | Wireless backhaul system |
| CA2905070A1 (en) | 2013-03-14 | 2014-09-25 | Genentech, Inc. | Methods of treating cancer and preventing cancer drug resistance |
| CN105617381B (zh) * | 2014-10-30 | 2019-07-05 | 中国科学院上海巴斯德研究所 | 组蛋白去乙酰化酶抑制剂治疗β亚科疱疹病毒的新用途 |
| WO2018213699A1 (en) * | 2017-05-19 | 2018-11-22 | Memorial Sloan Kettering Cancer Center | Methods for modifying endoplasmic reticulum processing of protein |
| CN111671742B (zh) * | 2020-05-17 | 2023-08-25 | 中国人民解放军军事科学院军事医学研究院 | 丙戊酸钠在制备人冠状病毒感染肺炎治疗药物中的应用 |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1999011659A1 (fr) * | 1997-09-02 | 1999-03-11 | Japan Energy Corporation | Derives tetrapeptides cycliques et leur utilisation medicinale |
| US6518012B1 (en) * | 1999-04-02 | 2003-02-11 | Health Research, Inc. | Method for regulating the expression of MHC antigens and CD40 by inhibitors of histone deacetylation |
| EP1170008A1 (en) * | 2000-07-07 | 2002-01-09 | Chemotherapeutisches Forschungsinstitut Georg-Speyer-Haus | Valproic acid and derivatives thereof as histone deacetylase inhibitors |
| EP1293205A1 (en) * | 2001-09-18 | 2003-03-19 | G2M Cancer Drugs AG | Valproic acid and derivatives thereof for the combination therapy of human cancers, for the treatment of tumour metastasis and minimal residual disease |
| ES2308065T3 (es) * | 2004-04-30 | 2008-12-01 | Topotarget Germany Ag | Formulacion que comprende un inhibidor de histona desacetilasa, que exhibe una liberacion bifasica. |
| EP2018366A4 (en) * | 2006-05-16 | 2010-08-04 | Univ Mcgill | HYBRID MOLECULES HAVING MIXED PROPERTIES OF VITAMIN D RECEPTOR AGONISM AND HISTONE DEACETYLASE INHIBITOR |
-
2007
- 2007-12-06 AU AU2007328872A patent/AU2007328872A1/en not_active Abandoned
- 2007-12-06 JP JP2009524035A patent/JP4887427B2/ja not_active Expired - Fee Related
- 2007-12-06 EP EP07850583A patent/EP2091525A1/en not_active Withdrawn
- 2007-12-06 US US12/448,073 patent/US20100093862A1/en not_active Abandoned
- 2007-12-06 CN CN200780044958A patent/CN101626763A/zh active Pending
- 2007-12-06 CA CA002671649A patent/CA2671649A1/en not_active Abandoned
- 2007-12-06 WO PCT/JP2007/074067 patent/WO2008069349A1/en active Application Filing
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP2010511597A5 (ja) | ||
| Sartorius et al. | The regulatory role of microRNA in hepatitis-B virus-associated hepatocellular carcinoma (HBV-HCC) pathogenesis | |
| Ghose et al. | Regulation of miR-146a by RelA/NFkB and p53 in ST HdhQ111/HdhQ111 Cells, a Cell Model of Huntington's Disease | |
| Inchley et al. | Nasal mucosal microRNA expression in children with respiratory syncytial virus infection | |
| Levene et al. | Host shutoff in influenza A virus: many means to an end | |
| Mrakovcic et al. | Epigenetic targeting of autophagy via HDAC inhibition in tumor cells: role of p53 | |
| ATE503770T1 (de) | Humane monoklonale antikörper gegen hendra- und nipah-viren | |
| BR112012025568A2 (pt) | proteínas de ligação ao tnf-<244>. | |
| WO2012103250A3 (en) | Colon cancer gene expression signatures and methods of use | |
| WO2012097313A3 (en) | Therapeutic antibodies against ror-1 protein and methods for use of same | |
| WO2009149026A3 (en) | Genomic approaches to fetal treatment and diagnosis | |
| BRPI1006270A8 (pt) | ''anticorpo anti a5b1, molécula de ácido nucleio isolado, célula hospedeira, método de produção de um anticorpo anti a5b1, imunoconjugado, composição farmacêutica, método de detecção de proteína a5b1, método de tratamento de um indivíduo e kit de detecção de a5b1 | |
| Vangimalla et al. | Bifunctional enzyme JMJD6 contributes to multiple disease pathogenesis: new twist on the old story | |
| Arechederra et al. | Epigenetic biomarkers for the diagnosis and treatment of liver disease | |
| DE602008006254D1 (de) | Doppel-oligonukleotid-nukleinsäurenachweisverfahren | |
| Czaja | next‐generation transformative advances in the pathogenesis and management of autoimmune hepatitis | |
| WO2018136919A3 (en) | Methods relating to lung cancer | |
| Ding et al. | HCV infection induces the upregulation of miR-221 in NF-κB dependent manner | |
| JP2013505007A5 (ja) | ||
| BR112016000874A2 (pt) | vírus de rna oncolítico geneticamente estável, método de fabricação e uso dos mesmos | |
| Yang et al. | Hepatitis B virus X protein mediated epigenetic alterations in the pathogenesis of hepatocellular carcinoma | |
| WO2013036622A3 (en) | Antiviral peptides effective against hepatitis c virus | |
| WO2009029681A3 (en) | Micrornas for inhibiting viral replication | |
| WO2010127252A3 (en) | Monoclonal antibodies to influenza h1n1 virus and uses thereof | |
| Castro-Oropeza et al. | Epigenetic and transcriptomic regulation landscape in HPV+ cancers: biological and clinical implications |