JP2010207237A5 - - Google Patents
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- Publication number
- JP2010207237A5 JP2010207237A5 JP2010104811A JP2010104811A JP2010207237A5 JP 2010207237 A5 JP2010207237 A5 JP 2010207237A5 JP 2010104811 A JP2010104811 A JP 2010104811A JP 2010104811 A JP2010104811 A JP 2010104811A JP 2010207237 A5 JP2010207237 A5 JP 2010207237A5
- Authority
- JP
- Japan
- Prior art keywords
- capillary
- reaction mixture
- capillary electrophoresis
- amplification
- pcr
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000005251 capillar electrophoresis Methods 0.000 claims description 48
- 230000003321 amplification Effects 0.000 claims description 36
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 36
- 238000004458 analytical method Methods 0.000 claims description 21
- 108091033319 polynucleotide Proteins 0.000 claims description 19
- 102000040430 polynucleotide Human genes 0.000 claims description 19
- 239000002157 polynucleotide Substances 0.000 claims description 19
- 238000001962 electrophoresis Methods 0.000 claims description 6
- 238000000034 method Methods 0.000 claims description 3
- 239000011541 reaction mixture Substances 0.000 claims 46
- 238000012408 PCR amplification Methods 0.000 claims 34
- 238000003752 polymerase chain reaction Methods 0.000 claims 23
- 102000039446 nucleic acids Human genes 0.000 claims 14
- 108020004707 nucleic acids Proteins 0.000 claims 14
- 150000007523 nucleic acids Chemical class 0.000 claims 14
- 238000000926 separation method Methods 0.000 claims 7
- 238000007654 immersion Methods 0.000 claims 5
- 238000000605 extraction Methods 0.000 claims 4
- 239000002184 metal Substances 0.000 claims 2
- 238000010839 reverse transcription Methods 0.000 claims 2
- 108091032973 (ribonucleotides)n+m Proteins 0.000 claims 1
- 239000012620 biological material Substances 0.000 claims 1
- 239000002299 complementary DNA Substances 0.000 claims 1
- 238000001816 cooling Methods 0.000 claims 1
- 239000007850 fluorescent dye Substances 0.000 claims 1
- 238000010438 heat treatment Methods 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 claims 1
- 108020004999 messenger RNA Proteins 0.000 claims 1
- 239000003792 electrolyte Substances 0.000 description 6
- 239000012530 fluid Substances 0.000 description 4
- 238000005370 electroosmosis Methods 0.000 description 2
- 238000005086 pumping Methods 0.000 description 2
- 230000005684 electric field Effects 0.000 description 1
- 125000000524 functional group Chemical group 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US39026902P | 2002-06-20 | 2002-06-20 | |
| US60/390,269 | 2002-06-20 |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2004516034A Division JP2005529626A (ja) | 2002-06-20 | 2003-06-19 | ポリヌクレオチドの検出および定量装置 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2010207237A JP2010207237A (ja) | 2010-09-24 |
| JP2010207237A5 true JP2010207237A5 (enExample) | 2012-04-12 |
| JP5258835B2 JP5258835B2 (ja) | 2013-08-07 |
Family
ID=30000534
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2004516034A Pending JP2005529626A (ja) | 2002-06-20 | 2003-06-19 | ポリヌクレオチドの検出および定量装置 |
| JP2010104811A Expired - Fee Related JP5258835B2 (ja) | 2002-06-20 | 2010-04-30 | ポリヌクレオチドの検出及び定量装置 |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2004516034A Pending JP2005529626A (ja) | 2002-06-20 | 2003-06-19 | ポリヌクレオチドの検出および定量装置 |
Country Status (8)
| Country | Link |
|---|---|
| US (4) | US20040014117A1 (enExample) |
| EP (1) | EP1532273A4 (enExample) |
| JP (2) | JP2005529626A (enExample) |
| KR (1) | KR101086611B1 (enExample) |
| CN (2) | CN100396789C (enExample) |
| AU (2) | AU2003243691A1 (enExample) |
| CA (1) | CA2490197A1 (enExample) |
| WO (1) | WO2004001376A2 (enExample) |
Families Citing this family (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7846315B2 (en) * | 2002-01-28 | 2010-12-07 | Qiagen Sciences, Llc | Integrated bio-analysis and sample preparation system |
| JP4501430B2 (ja) * | 2004-01-08 | 2010-07-14 | カシオ計算機株式会社 | 検査装置、検査方法及び合成装置 |
| US20070295917A1 (en) * | 2004-01-26 | 2007-12-27 | Applera Corporation | Single excitation wavelength fluorescent detection system |
| TWI391492B (zh) * | 2007-11-20 | 2013-04-01 | Quanta Comp Inc | 自動化遺傳物質處理系統及方法 |
| WO2010141131A1 (en) | 2009-06-04 | 2010-12-09 | Lockheed Martin Corporation | Multiple-sample microfluidic chip for dna analysis |
| CA2814720C (en) | 2010-10-15 | 2016-12-13 | Lockheed Martin Corporation | Micro fluidic optic design |
| JP5722001B2 (ja) * | 2010-11-10 | 2015-05-20 | 株式会社日立ハイテクノロジーズ | 遺伝子検査方法及び検査装置 |
| US9506117B2 (en) | 2012-02-21 | 2016-11-29 | Oslo Universitetssykehus Hf | Methods and biomarkers for detection and prognosis of cervical cancer |
| US9322054B2 (en) | 2012-02-22 | 2016-04-26 | Lockheed Martin Corporation | Microfluidic cartridge |
| CN103352002B (zh) * | 2012-07-05 | 2015-09-02 | 卡尤迪生物科技（北京）有限公司 | 基因扩增及基因检测一体化系统 |
| WO2014039963A1 (en) * | 2012-09-10 | 2014-03-13 | Biofire Diagnostics, Inc. | Multiple amplification cycle detection |
| DE102013200193A1 (de) * | 2013-01-09 | 2014-07-10 | Hamilton Bonaduz Ag | Probenverarbeitungssystem mit Dosiervorrichtung und Thermocycler |
| US10844424B2 (en) | 2013-02-20 | 2020-11-24 | Bionano Genomics, Inc. | Reduction of bias in genomic coverage measurements |
| EP2959015B1 (en) | 2013-02-20 | 2020-11-04 | Bionano Genomics, Inc. | Characterization of molecules in nanofluidics |
| GB201401584D0 (en) * | 2014-01-29 | 2014-03-19 | Bg Res Ltd | Intelligent detection of biological entities |
| CN106164295B (zh) | 2014-02-25 | 2020-08-11 | 生物纳米基因公司 | 减小基因组覆盖测量中的偏差 |
| CN105022900B (zh) * | 2015-08-19 | 2018-02-09 | 电子科技大学 | 基于热固耦合分析的重型数控立车静压转台结构优化方法 |
| CN108359604A (zh) * | 2018-01-25 | 2018-08-03 | 山东百多安医疗器械有限公司 | 一种干细胞种子输送及微环境调节系统 |
| JP2022515782A (ja) | 2018-12-19 | 2022-02-22 | ニュークレイン リミテッド ライアビリティ カンパニー | 分子診断のための装置および方法 |
Family Cites Families (33)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0311440B1 (en) * | 1987-10-09 | 1992-06-24 | Seiko Instruments Inc. | Apparatus for carrying out a liquid reaction |
| US5169511A (en) * | 1988-11-29 | 1992-12-08 | Isco, Inc. | Capillary electrophoresis technique |
| CA2031912A1 (en) * | 1989-12-22 | 1991-06-23 | Robert Fred Pfost | Heated cover device |
| US5935522A (en) * | 1990-06-04 | 1999-08-10 | University Of Utah Research Foundation | On-line DNA analysis system with rapid thermal cycling |
| JP3011987B2 (ja) * | 1990-10-11 | 2000-02-21 | 旭化成工業株式会社 | 固相化プライマーを用いる逆転写酵素の測定法 |
| KR100236506B1 (ko) * | 1990-11-29 | 2000-01-15 | 퍼킨-엘머시터스인스트루먼츠 | 폴리머라제 연쇄 반응 수행 장치 |
| US6403367B1 (en) * | 1994-07-07 | 2002-06-11 | Nanogen, Inc. | Integrated portable biological detection system |
| US5498545A (en) * | 1994-07-21 | 1996-03-12 | Vestal; Marvin L. | Mass spectrometer system and method for matrix-assisted laser desorption measurements |
| DE69525036T2 (de) * | 1994-11-14 | 2002-08-01 | Trustees Of The University Of Pennsylvania, Philadelphia | Gerät zur amplifikation von polynukleotiden im mesomassstab |
| US5710628A (en) * | 1994-12-12 | 1998-01-20 | Visible Genetics Inc. | Automated electrophoresis and fluorescence detection apparatus and method |
| US6168948B1 (en) * | 1995-06-29 | 2001-01-02 | Affymetrix, Inc. | Miniaturized genetic analysis systems and methods |
| US5856174A (en) * | 1995-06-29 | 1999-01-05 | Affymetrix, Inc. | Integrated nucleic acid diagnostic device |
| US5776699A (en) * | 1995-09-01 | 1998-07-07 | Allergan, Inc. | Method of identifying negative hormone and/or antagonist activities |
| US5567294A (en) * | 1996-01-30 | 1996-10-22 | Board Of Governors, University Of Alberta | Multiple capillary biochemical analyzer with barrier member |
| EP0909389A2 (en) * | 1996-07-05 | 1999-04-21 | Beckman Coulter, Inc. | Automated sample processing system |
| JP4000605B2 (ja) * | 1996-07-24 | 2007-10-31 | 株式会社日立製作所 | Ｄｎａ試料調整装置及びこれを用いる電気泳動分析装置 |
| US6027627A (en) * | 1997-06-30 | 2000-02-22 | Spectrumedix Corporation | Automated parallel capillary electrophoretic system |
| US6207031B1 (en) * | 1997-09-15 | 2001-03-27 | Whitehead Institute For Biomedical Research | Methods and apparatus for processing a sample of biomolecular analyte using a microfabricated device |
| US6126804A (en) * | 1997-09-23 | 2000-10-03 | The Regents Of The University Of California | Integrated polymerase chain reaction/electrophoresis instrument |
| US6979424B2 (en) * | 1998-03-17 | 2005-12-27 | Cepheid | Integrated sample analysis device |
| JP3713970B2 (ja) * | 1998-09-09 | 2005-11-09 | 株式会社日立製作所 | 特異的遺伝子断片の分離採取装置 |
| US6372484B1 (en) * | 1999-01-25 | 2002-04-16 | E.I. Dupont De Nemours And Company | Apparatus for integrated polymerase chain reaction and capillary electrophoresis |
| JP2000346828A (ja) * | 1999-06-02 | 2000-12-15 | Hitachi Ltd | 電気泳動装置 |
| JP4071907B2 (ja) * | 1999-11-29 | 2008-04-02 | オリンパス株式会社 | 自動核酸検査装置 |
| EP1276900A2 (en) * | 2000-01-11 | 2003-01-22 | Maxygen, Inc. | Integrated systems and methods for diversity generation and screening |
| JP4103302B2 (ja) * | 2000-05-15 | 2008-06-18 | 株式会社日立製作所 | キャピラリアレイを用いた電気泳動装置及びそれに用いられるサンプルプレートアセンブリ |
| US6783649B2 (en) * | 2000-12-01 | 2004-08-31 | Cetek Corporation | High throughput capillary electrophoresis system |
| DE60220497T2 (de) * | 2001-01-26 | 2008-01-31 | Biocal Technology, Inc., Irvine | Optische detektion in einem mehrkanaligen bioseparationssystem |
| JP2004532384A (ja) * | 2001-01-26 | 2004-10-21 | バイオカル テクノロジー，インコーポレイティド | マルチチャネル生物分離カートリッジ |
| US20040266021A9 (en) * | 2001-01-26 | 2004-12-30 | Andras Guttman | Multicapillary fraction collection system and method |
| US7250099B2 (en) * | 2002-12-13 | 2007-07-31 | Biocal Technology, Inc. | Optical detection alignment coupling apparatus |
| US7846315B2 (en) * | 2002-01-28 | 2010-12-07 | Qiagen Sciences, Llc | Integrated bio-analysis and sample preparation system |
| US7445893B2 (en) * | 2002-04-12 | 2008-11-04 | Primera Biosystems, Inc. | Sampling method for amplification reaction analysis |
-
2003
- 2003-06-19 US US10/464,941 patent/US20040014117A1/en not_active Abandoned
- 2003-06-19 KR KR1020047020741A patent/KR101086611B1/ko not_active Expired - Fee Related
- 2003-06-19 CN CNB038172453A patent/CN100396789C/zh not_active Expired - Fee Related
- 2003-06-19 AU AU2003243691A patent/AU2003243691A1/en not_active Abandoned
- 2003-06-19 JP JP2004516034A patent/JP2005529626A/ja active Pending
- 2003-06-19 EP EP03761193A patent/EP1532273A4/en not_active Ceased
- 2003-06-19 CA CA002490197A patent/CA2490197A1/en not_active Abandoned
- 2003-06-19 CN CN2008100947129A patent/CN101348763B/zh not_active Expired - Fee Related
- 2003-06-19 WO PCT/US2003/019518 patent/WO2004001376A2/en not_active Ceased
-
2009
- 2009-04-20 AU AU2009201529A patent/AU2009201529B2/en not_active Ceased
-
2010
- 2010-04-30 JP JP2010104811A patent/JP5258835B2/ja not_active Expired - Fee Related
-
2011
- 2011-12-06 US US13/312,584 patent/US20120100600A1/en not_active Abandoned
-
2012
- 2012-12-17 US US13/716,615 patent/US20130189768A1/en not_active Abandoned
-
2014
- 2014-01-13 US US14/153,600 patent/US20140256029A1/en not_active Abandoned
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