JP2010207192A - 電気培養方法及び電気培養装置 - Google Patents
電気培養方法及び電気培養装置 Download PDFInfo
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Abstract
【解決手段】本発明の電気培養装置1は、イオン交換膜6によって仕切られ且つ開放された二つの槽(培養槽7と対電極槽8)と、作用電極9及び対電極10と、作用電極9と対電極10との間に電位差を与える電源12とを有し、培養槽7には酸化還元物質3を含む培養液4が収容されると共に作用電極9が培養液4に浸され、対電極槽8には電解液4aが収容されると共に対電極10が電解液4aに浸され、培養液4に培養対象たる微生物2が添加されて、電源12により作用電極9と対電極10との間に電位差を与えながら微生物を培養する電気培養装置において、培養槽7を、対電極槽8から発生するガスが培養槽7へ侵入するのを防ぐ密閉構造とした。
【選択図】図1
Description
[数式1] E=E0+RT/nF×ln(Cox/Cred)
図6に示す電気培養装置1により実験を行った。培養槽7としての容器20は250mL容のガラスバイアル瓶(Duran製)とした。培養液4は容器20の八分目程度まで入れた。容器20には蓋30をした。蓋30の上面30aにはシリコーンゴム栓を設けて、配線や電極、管と通した際の密閉性を確保した。また、シリコーンゴム栓を設けることにより注射針の突き刺しを可能とし、且つ注射針の差し込みにより生じた孔が注射針を抜いた際に塞がるようにした。
微生物2として、Clostridium acetobutylicum NBRC13948(以下、単にクロストリジウムと呼ぶ)を用いた。この微生物は、無酸素条件下でグルコースからエタノール、ブタノール、アセトン、酢酸、酪酸を生成することのできる偏性嫌気性微生物である。培養温度は30℃とした。また、酸化還元物質3としてFe(III)−EDTAを用い、培養液4の酸化還元物質濃度は2mMとした。実験に使用した培養液4の構成成分を表1に示す。また、電解液4aの組成は、表1の組成からFe(III)−EDTAを除いたものとした。尚、培養液4は攪拌子34で攪拌し、蓋30に備えられたシリコーンゴム栓に注射針を2本刺し、一方の注射針からN2ガスを1時間通気することにより、ヘッドスペースをN2ガスで置換した。
2 培養対象微生物
3 酸化還元物質
4 培養液
4a 電解液
6 イオン交換膜
7 培養槽
8 対電極槽
9 作用電極
10 対電極
12 電源(電位制御装置)
15 ガス回収手段
16 培養液採取手段
20 容器
21 小容器
22 ガス排出管
23 容器
24 ガス不透過膜、ガス不透過部材
25 U字型構造の容器
25a U字型構造の容器を構成する一方の容器
25b U字型構造の容器を構成する他方の容器
26 H字型構造の容器
26a H字型構造の容器を構成する一方の容器
26b H字型構造の容器を構成する他方の容器
Claims (11)
- イオン交換膜によって仕切られ且つ開放されている二つの槽のうち、一方の槽を培養槽として酸化還元物質を含む培養液を収容すると共に前記培養液に作用電極を浸し、他方の槽を対電極槽として電解液を収容すると共に前記電解液に対電極を浸し、前記培養槽に培養対象たる微生物を添加して、前記作用電極と前記対電極との間に電位差を与えながら前記微生物を培養する電気培養方法において、前記培養槽を密閉し、前記対電極槽から発生するガスが前記培養槽に侵入するのを防ぎながら前記微生物を培養することを特徴とする電気培養方法。
- 請求項1に記載の電気培養方法によりガス状物質を生産する微生物または揮発性物質を生産する微生物を培養し、前記ガス状物質または前記揮発性物質が揮発して発生したガス成分を前記培養槽内の前記培養液の液面よりも上部の空間に滞留させ、これを回収することを特徴とする物質生産方法。
- 請求項1に記載の電気培養方法により揮発性物質を生産する微生物を培養し、前記微生物が生産する前記揮発性物質を前記培養槽内の前記培養液から回収することを特徴とする物質生産方法。
- 請求項1に記載の電気培養方法により揮発性の有害物質を分解する微生物を培養し、前記培養槽内の前記培養液に添加された前記有害物質を分解処理することを特徴とする物質分解処理方法。
- イオン交換膜によって仕切られ且つ開放された二つの槽と、作用電極及び対電極と、前記作用電極と前記対電極との間に電位差を与える電源とを有し、前記二つの槽のうちの一方の槽を培養槽として酸化還元物質を含む培養液が収容されると共に前記作用電極が前記培養液に浸され、前記二つの槽のうちの他方の槽を対電極槽として電解液が収容されると共に前記対電極が前記電解液に浸され、前記培養液に培養対象たる微生物が添加されて、前記電源により前記作用電極と前記対電極との間に電位差を与えながら前記微生物を培養する電気培養装置において、前記培養槽を、前記対電極槽から発生するガスが前記培養槽へ侵入するのを防ぐ密閉構造としたことを特徴とする電気培養装置。
- 密閉構造の容器を前記培養槽とし、前記容器に収容可能な密閉構造の小容器を前記対電極槽とし、前記小容器は少なくとも一部に前記イオン交換膜を備えると共に前記ガスを前記容器の外に排出するガス排出管を備えるものとしている請求項5に記載の電気培養装置。
- 上方が開放されている容器を前記イオン交換膜で仕切ることにより前記開放された二つの槽が形成され、前記培養槽としての前記一方の槽の上方開放部がガス不透過膜またはガス不透過部材により塞がれている請求項5に記載の電気培養装置。
- 収容される液体の液面よりも下部に開口部を備える二つの容器が前記イオン交換膜を介して前記開口部で連結されてU字型あるいはH字型の容器が形成され、前記二つの容器のうちの一方の容器を密閉構造として前記培養槽とし、他方の容器を開放して前記対電極槽としている請求項5に記載の電気培養装置。
- 前記対電極槽に前記電解液を収容せずに前記対電極を前記イオン交換膜と接触させ、且つ前記対電極を多孔性としている請求項5に記載の電気培養装置。
- 前記培養槽に収容されている前記培養液の液面よりも上部の空間に滞留するガス状物質を回収するガス回収手段を備える請求項5に記載の電気培養装置。
- 前記培養槽に収容されている前記培養液を採取する培養液採取手段を備える請求項5に記載の電気培養装置。
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KR101180340B1 (ko) | 2010-12-15 | 2012-09-07 | 한국원자력연구원 | 이산화탄소 고정화 세균의 농화배양을 위한 전기화학반응기 |
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