JP5832766B2 - 微生物の代謝経路制御方法 - Google Patents
微生物の代謝経路制御方法 Download PDFInfo
- Publication number
- JP5832766B2 JP5832766B2 JP2011067404A JP2011067404A JP5832766B2 JP 5832766 B2 JP5832766 B2 JP 5832766B2 JP 2011067404 A JP2011067404 A JP 2011067404A JP 2011067404 A JP2011067404 A JP 2011067404A JP 5832766 B2 JP5832766 B2 JP 5832766B2
- Authority
- JP
- Japan
- Prior art keywords
- electrode
- potential
- culture
- pathway
- container
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 244000005700 microbiome Species 0.000 title claims description 58
- 230000037353 metabolic pathway Effects 0.000 title claims description 47
- 238000000034 method Methods 0.000 title claims description 29
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 116
- 238000006722 reduction reaction Methods 0.000 claims description 69
- 238000007254 oxidation reaction Methods 0.000 claims description 65
- 230000037361 pathway Effects 0.000 claims description 60
- ZSLZBFCDCINBPY-ZSJPKINUSA-N acetyl-CoA Chemical compound O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(=O)OP(O)(=O)OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCSC(=O)C)O[C@H]1N1C2=NC=NC(N)=C2N=C1 ZSLZBFCDCINBPY-ZSJPKINUSA-N 0.000 claims description 58
- 239000004310 lactic acid Substances 0.000 claims description 58
- 235000014655 lactic acid Nutrition 0.000 claims description 58
- 230000009467 reduction Effects 0.000 claims description 48
- 230000003647 oxidation Effects 0.000 claims description 45
- LCTONWCANYUPML-UHFFFAOYSA-M Pyruvate Chemical compound CC(=O)C([O-])=O LCTONWCANYUPML-UHFFFAOYSA-M 0.000 claims description 44
- 229940100228 acetyl coenzyme a Drugs 0.000 claims description 29
- 241000894006 Bacteria Species 0.000 claims description 19
- 241000588724 Escherichia coli Species 0.000 claims description 16
- 230000001590 oxidative effect Effects 0.000 claims description 13
- 230000003301 hydrolyzing effect Effects 0.000 claims description 12
- 229910021607 Silver chloride Inorganic materials 0.000 claims description 10
- 229910052709 silver Inorganic materials 0.000 claims description 10
- 239000004332 silver Substances 0.000 claims description 10
- HKZLPVFGJNLROG-UHFFFAOYSA-M silver monochloride Chemical compound [Cl-].[Ag+] HKZLPVFGJNLROG-UHFFFAOYSA-M 0.000 claims description 10
- 238000012258 culturing Methods 0.000 claims description 7
- 230000001737 promoting effect Effects 0.000 claims description 6
- 239000000243 solution Substances 0.000 description 99
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 78
- 239000007789 gas Substances 0.000 description 75
- 239000003014 ion exchange membrane Substances 0.000 description 62
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 57
- LCTONWCANYUPML-UHFFFAOYSA-N Pyruvic acid Chemical compound CC(=O)C(O)=O LCTONWCANYUPML-UHFFFAOYSA-N 0.000 description 46
- 229940076788 pyruvate Drugs 0.000 description 41
- 229940107700 pyruvic acid Drugs 0.000 description 23
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 description 20
- 239000000126 substance Substances 0.000 description 19
- 230000000694 effects Effects 0.000 description 18
- 238000004519 manufacturing process Methods 0.000 description 18
- 239000000463 material Substances 0.000 description 17
- 239000008151 electrolyte solution Substances 0.000 description 16
- 239000007788 liquid Substances 0.000 description 16
- 238000011084 recovery Methods 0.000 description 14
- 230000007423 decrease Effects 0.000 description 13
- 239000012528 membrane Substances 0.000 description 12
- 239000001257 hydrogen Substances 0.000 description 11
- 229910052739 hydrogen Inorganic materials 0.000 description 11
- 239000002207 metabolite Substances 0.000 description 11
- 229920002379 silicone rubber Polymers 0.000 description 11
- 239000004945 silicone rubber Substances 0.000 description 11
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 10
- 241000204652 Thermotoga Species 0.000 description 10
- 230000002829 reductive effect Effects 0.000 description 9
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 8
- 238000012360 testing method Methods 0.000 description 8
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 7
- 229920000557 Nafion® Polymers 0.000 description 7
- 229910052799 carbon Inorganic materials 0.000 description 7
- 230000008859 change Effects 0.000 description 7
- 230000033116 oxidation-reduction process Effects 0.000 description 7
- 229920002472 Starch Polymers 0.000 description 6
- MSSUFHMGCXOVBZ-UHFFFAOYSA-N anthraquinone-2,6-disulfonic acid Chemical compound OS(=O)(=O)C1=CC=C2C(=O)C3=CC(S(=O)(=O)O)=CC=C3C(=O)C2=C1 MSSUFHMGCXOVBZ-UHFFFAOYSA-N 0.000 description 6
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 6
- 230000003247 decreasing effect Effects 0.000 description 6
- 238000000855 fermentation Methods 0.000 description 6
- 230000004151 fermentation Effects 0.000 description 6
- 239000011521 glass Substances 0.000 description 6
- 238000002347 injection Methods 0.000 description 6
- 239000007924 injection Substances 0.000 description 6
- 239000000203 mixture Substances 0.000 description 6
- 239000008107 starch Substances 0.000 description 6
- 235000019698 starch Nutrition 0.000 description 6
- 230000036962 time dependent Effects 0.000 description 6
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 5
- 239000006185 dispersion Substances 0.000 description 5
- 239000013013 elastic material Substances 0.000 description 5
- 239000001963 growth medium Substances 0.000 description 5
- XEEYBQQBJWHFJM-UHFFFAOYSA-N iron Substances [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 5
- 229910052742 iron Inorganic materials 0.000 description 5
- -1 iron ions Chemical class 0.000 description 5
- 238000005259 measurement Methods 0.000 description 5
- 239000011734 sodium Substances 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 4
- 241000204666 Thermotoga maritima Species 0.000 description 4
- 239000002253 acid Substances 0.000 description 4
- 230000008901 benefit Effects 0.000 description 4
- 229920000704 biodegradable plastic Polymers 0.000 description 4
- 239000002551 biofuel Substances 0.000 description 4
- 238000000354 decomposition reaction Methods 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 150000002500 ions Chemical class 0.000 description 4
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 4
- 229920000747 poly(lactic acid) Polymers 0.000 description 4
- 239000004626 polylactic acid Substances 0.000 description 4
- 239000002994 raw material Substances 0.000 description 4
- 239000011780 sodium chloride Substances 0.000 description 4
- 239000011573 trace mineral Substances 0.000 description 4
- 235000013619 trace mineral Nutrition 0.000 description 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 3
- QPCDCPDFJACHGM-UHFFFAOYSA-N N,N-bis{2-[bis(carboxymethyl)amino]ethyl}glycine Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(=O)O)CCN(CC(O)=O)CC(O)=O QPCDCPDFJACHGM-UHFFFAOYSA-N 0.000 description 3
- MUBZPKHOEPUJKR-UHFFFAOYSA-N Oxalic acid Chemical compound OC(=O)C(O)=O MUBZPKHOEPUJKR-UHFFFAOYSA-N 0.000 description 3
- 239000000853 adhesive Substances 0.000 description 3
- 230000001070 adhesive effect Effects 0.000 description 3
- 239000002738 chelating agent Substances 0.000 description 3
- 239000003792 electrolyte Substances 0.000 description 3
- 239000008103 glucose Substances 0.000 description 3
- 230000007774 longterm Effects 0.000 description 3
- 229910052751 metal Inorganic materials 0.000 description 3
- 239000002184 metal Substances 0.000 description 3
- 239000005416 organic matter Substances 0.000 description 3
- 239000004033 plastic Substances 0.000 description 3
- 229920003023 plastic Polymers 0.000 description 3
- 230000006641 stabilisation Effects 0.000 description 3
- 238000011105 stabilization Methods 0.000 description 3
- 229920003051 synthetic elastomer Polymers 0.000 description 3
- 239000005061 synthetic rubber Substances 0.000 description 3
- IYNGLSRZLOHZJA-UHFFFAOYSA-N 1,4,7-triazabicyclo[5.2.2]undecane Chemical compound C1CN2CCN1CCNCC2 IYNGLSRZLOHZJA-UHFFFAOYSA-N 0.000 description 2
- XMTQQYYKAHVGBJ-UHFFFAOYSA-N 3-(3,4-DICHLOROPHENYL)-1,1-DIMETHYLUREA Chemical compound CN(C)C(=O)NC1=CC=C(Cl)C(Cl)=C1 XMTQQYYKAHVGBJ-UHFFFAOYSA-N 0.000 description 2
- QTXZASLUYMRUAN-QLQASOTGSA-N Acetyl coenzyme A (Acetyl-CoA) Chemical compound O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(=O)OP(O)(=O)OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCSC(=O)C)O[C@H]1N1C2=NC=NC(N)=C2N=C1.O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(=O)OP(O)(=O)OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCSC(=O)C)O[C@H]1N1C2=NC=NC(N)=C2N=C1 QTXZASLUYMRUAN-QLQASOTGSA-N 0.000 description 2
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- RPNUMPOLZDHAAY-UHFFFAOYSA-N Diethylenetriamine Chemical compound NCCNCCN RPNUMPOLZDHAAY-UHFFFAOYSA-N 0.000 description 2
- PIICEJLVQHRZGT-UHFFFAOYSA-N Ethylenediamine Chemical compound NCCN PIICEJLVQHRZGT-UHFFFAOYSA-N 0.000 description 2
- XUJNEKJLAYXESH-REOHCLBHSA-N L-Cysteine Chemical compound SC[C@H](N)C(O)=O XUJNEKJLAYXESH-REOHCLBHSA-N 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-M Lactate Chemical compound CC(O)C([O-])=O JVTAAEKCZFNVCJ-UHFFFAOYSA-M 0.000 description 2
- 244000199866 Lactobacillus casei Species 0.000 description 2
- 235000013958 Lactobacillus casei Nutrition 0.000 description 2
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 2
- 244000057717 Streptococcus lactis Species 0.000 description 2
- 235000014897 Streptococcus lactis Nutrition 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
- 241000999856 Thermotoga maritima MSB8 Species 0.000 description 2
- 241000193761 [Bacillus] caldolyticus Species 0.000 description 2
- 229910052782 aluminium Inorganic materials 0.000 description 2
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 239000005293 duran Substances 0.000 description 2
- 238000005868 electrolysis reaction Methods 0.000 description 2
- 150000002431 hydrogen Chemical class 0.000 description 2
- 238000005342 ion exchange Methods 0.000 description 2
- 229940017800 lactobacillus casei Drugs 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 230000004060 metabolic process Effects 0.000 description 2
- 230000007935 neutral effect Effects 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 238000005192 partition Methods 0.000 description 2
- 229960003330 pentetic acid Drugs 0.000 description 2
- 239000012466 permeate Substances 0.000 description 2
- 229910052697 platinum Inorganic materials 0.000 description 2
- 230000027756 respiratory electron transport chain Effects 0.000 description 2
- 230000000717 retained effect Effects 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 239000013535 sea water Substances 0.000 description 2
- 238000004904 shortening Methods 0.000 description 2
- JHJLBTNAGRQEKS-UHFFFAOYSA-M sodium bromide Chemical compound [Na+].[Br-] JHJLBTNAGRQEKS-UHFFFAOYSA-M 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- LJRDOKAZOAKLDU-UDXJMMFXSA-N (2s,3s,4r,5r,6r)-5-amino-2-(aminomethyl)-6-[(2r,3s,4r,5s)-5-[(1r,2r,3s,5r,6s)-3,5-diamino-2-[(2s,3r,4r,5s,6r)-3-amino-4,5-dihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy-6-hydroxycyclohexyl]oxy-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl]oxyoxane-3,4-diol;sulfuric ac Chemical compound OS(O)(=O)=O.N[C@@H]1[C@@H](O)[C@H](O)[C@H](CN)O[C@@H]1O[C@H]1[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](N)C[C@@H](N)[C@@H]2O)O[C@@H]2[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O2)N)O[C@@H]1CO LJRDOKAZOAKLDU-UDXJMMFXSA-N 0.000 description 1
- LXJXRIRHZLFYRP-VKHMYHEASA-L (R)-2-Hydroxy-3-(phosphonooxy)-propanal Natural products O=C[C@H](O)COP([O-])([O-])=O LXJXRIRHZLFYRP-VKHMYHEASA-L 0.000 description 1
- HNSDLXPSAYFUHK-UHFFFAOYSA-N 1,4-bis(2-ethylhexyl) sulfosuccinate Chemical compound CCCCC(CC)COC(=O)CC(S(O)(=O)=O)C(=O)OCC(CC)CCCC HNSDLXPSAYFUHK-UHFFFAOYSA-N 0.000 description 1
- VILCJCGEZXAXTO-UHFFFAOYSA-N 2,2,2-tetramine Chemical compound NCCNCCNCCN VILCJCGEZXAXTO-UHFFFAOYSA-N 0.000 description 1
- LJQLQCAXBUHEAZ-UWTATZPHSA-N 3-phospho-D-glyceroyl dihydrogen phosphate Chemical compound OP(=O)(O)OC[C@@H](O)C(=O)OP(O)(O)=O LJQLQCAXBUHEAZ-UWTATZPHSA-N 0.000 description 1
- IKHGUXGNUITLKF-UHFFFAOYSA-N Acetaldehyde Chemical compound CC=O IKHGUXGNUITLKF-UHFFFAOYSA-N 0.000 description 1
- 241000186044 Actinomyces viscosus Species 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 241000194107 Bacillus megaterium Species 0.000 description 1
- 244000063299 Bacillus subtilis Species 0.000 description 1
- 235000014469 Bacillus subtilis Nutrition 0.000 description 1
- 241000605900 Butyrivibrio fibrisolvens Species 0.000 description 1
- 241000206593 Carnobacterium divergens Species 0.000 description 1
- 241000193468 Clostridium perfringens Species 0.000 description 1
- LXJXRIRHZLFYRP-VKHMYHEASA-N D-glyceraldehyde 3-phosphate Chemical compound O=C[C@H](O)COP(O)(O)=O LXJXRIRHZLFYRP-VKHMYHEASA-N 0.000 description 1
- ZGTMUACCHSMWAC-UHFFFAOYSA-L EDTA disodium salt (anhydrous) Chemical compound [Na+].[Na+].OC(=O)CN(CC([O-])=O)CCN(CC(O)=O)CC([O-])=O ZGTMUACCHSMWAC-UHFFFAOYSA-L 0.000 description 1
- QZKRHPLGUJDVAR-UHFFFAOYSA-K EDTA trisodium salt Chemical compound [Na+].[Na+].[Na+].OC(=O)CN(CC([O-])=O)CCN(CC([O-])=O)CC([O-])=O QZKRHPLGUJDVAR-UHFFFAOYSA-K 0.000 description 1
- 241000194032 Enterococcus faecalis Species 0.000 description 1
- 241000194031 Enterococcus faecium Species 0.000 description 1
- 230000005526 G1 to G0 transition Effects 0.000 description 1
- 241000193385 Geobacillus stearothermophilus Species 0.000 description 1
- 241000588747 Klebsiella pneumoniae Species 0.000 description 1
- 239000004201 L-cysteine Substances 0.000 description 1
- 235000013878 L-cysteine Nutrition 0.000 description 1
- 241000186660 Lactobacillus Species 0.000 description 1
- 240000001929 Lactobacillus brevis Species 0.000 description 1
- 235000013957 Lactobacillus brevis Nutrition 0.000 description 1
- 241000186840 Lactobacillus fermentum Species 0.000 description 1
- 240000006024 Lactobacillus plantarum Species 0.000 description 1
- 235000013965 Lactobacillus plantarum Nutrition 0.000 description 1
- 241000194034 Lactococcus lactis subsp. cremoris Species 0.000 description 1
- 101100496858 Mus musculus Colec12 gene Proteins 0.000 description 1
- 241000187654 Nocardia Species 0.000 description 1
- 241000192134 Oenococcus oeni Species 0.000 description 1
- 241000605031 Selenomonas ruminantium Species 0.000 description 1
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 1
- 241000204115 Sporolactobacillus inulinus Species 0.000 description 1
- 241000191967 Staphylococcus aureus Species 0.000 description 1
- 241000194011 Streptococcus acidominimus Species 0.000 description 1
- 241000193985 Streptococcus agalactiae Species 0.000 description 1
- 235000014962 Streptococcus cremoris Nutrition 0.000 description 1
- 241000194049 Streptococcus equinus Species 0.000 description 1
- 241000194054 Streptococcus uberis Species 0.000 description 1
- 241000186337 Thermoanaerobacter ethanolicus Species 0.000 description 1
- 241000589501 Thermus caldophilus Species 0.000 description 1
- 241000589499 Thermus thermophilus Species 0.000 description 1
- 241000167552 Trichococcus pasteurii Species 0.000 description 1
- LIPOUNRJVLNBCD-UHFFFAOYSA-N acetyl dihydrogen phosphate Chemical compound CC(=O)OP(O)(O)=O LIPOUNRJVLNBCD-UHFFFAOYSA-N 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- BHRQIJRLOVHRKH-UHFFFAOYSA-L calcium;2-[bis[2-[bis(carboxylatomethyl)amino]ethyl]amino]acetate;hydron Chemical compound [Ca+2].OC(=O)CN(CC(O)=O)CCN(CC([O-])=O)CCN(CC(O)=O)CC([O-])=O BHRQIJRLOVHRKH-UHFFFAOYSA-L 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 238000005341 cation exchange Methods 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 150000003983 crown ethers Chemical class 0.000 description 1
- 238000000502 dialysis Methods 0.000 description 1
- 229910001873 dinitrogen Inorganic materials 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- UZVGFAUPMODEBR-UHFFFAOYSA-L disodium;9,10-dioxoanthracene-1,2-disulfonate Chemical compound [Na+].[Na+].C1=CC=C2C(=O)C3=C(S([O-])(=O)=O)C(S(=O)(=O)[O-])=CC=C3C(=O)C2=C1 UZVGFAUPMODEBR-UHFFFAOYSA-L 0.000 description 1
- 238000011549 displacement method Methods 0.000 description 1
- 229940009662 edetate Drugs 0.000 description 1
- 229960001484 edetic acid Drugs 0.000 description 1
- 238000002848 electrochemical method Methods 0.000 description 1
- 238000003487 electrochemical reaction Methods 0.000 description 1
- 229940032049 enterococcus faecalis Drugs 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 238000004817 gas chromatography Methods 0.000 description 1
- 238000009413 insulation Methods 0.000 description 1
- 238000004255 ion exchange chromatography Methods 0.000 description 1
- 229940039696 lactobacillus Drugs 0.000 description 1
- 229940012969 lactobacillus fermentum Drugs 0.000 description 1
- 229940072205 lactobacillus plantarum Drugs 0.000 description 1
- 238000004811 liquid chromatography Methods 0.000 description 1
- 239000013028 medium composition Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000000465 moulding Methods 0.000 description 1
- 230000003472 neutralizing effect Effects 0.000 description 1
- MGFYIUFZLHCRTH-UHFFFAOYSA-N nitrilotriacetic acid Chemical compound OC(=O)CN(CC(O)=O)CC(O)=O MGFYIUFZLHCRTH-UHFFFAOYSA-N 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 235000006408 oxalic acid Nutrition 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- FIKAKWIAUPDISJ-UHFFFAOYSA-L paraquat dichloride Chemical compound [Cl-].[Cl-].C1=C[N+](C)=CC=C1C1=CC=[N+](C)C=C1 FIKAKWIAUPDISJ-UHFFFAOYSA-L 0.000 description 1
- 230000035515 penetration Effects 0.000 description 1
- 229960001639 penicillamine Drugs 0.000 description 1
- 229940067082 pentetate Drugs 0.000 description 1
- 229940009784 pentetate calcium Drugs 0.000 description 1
- 229930029653 phosphoenolpyruvate Natural products 0.000 description 1
- DTBNBXWJWCWCIK-UHFFFAOYSA-N phosphoenolpyruvic acid Chemical compound OC(=O)C(=C)OP(O)(O)=O DTBNBXWJWCWCIK-UHFFFAOYSA-N 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 239000000276 potassium ferrocyanide Substances 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 150000004728 pyruvic acid derivatives Chemical class 0.000 description 1
- 150000004053 quinones Chemical class 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
- 229910052710 silicon Inorganic materials 0.000 description 1
- 239000010703 silicon Substances 0.000 description 1
- 229940037001 sodium edetate Drugs 0.000 description 1
- 238000000638 solvent extraction Methods 0.000 description 1
- 229940115921 streptococcus equinus Drugs 0.000 description 1
- 229940115922 streptococcus uberis Drugs 0.000 description 1
- 229960005346 succimer Drugs 0.000 description 1
- ACTRVOBWPAIOHC-XIXRPRMCSA-N succimer Chemical compound OC(=O)[C@@H](S)[C@@H](S)C(O)=O ACTRVOBWPAIOHC-XIXRPRMCSA-N 0.000 description 1
- XOGGUFAVLNCTRS-UHFFFAOYSA-N tetrapotassium;iron(2+);hexacyanide Chemical compound [K+].[K+].[K+].[K+].[Fe+2].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-] XOGGUFAVLNCTRS-UHFFFAOYSA-N 0.000 description 1
- 229960001124 trientine Drugs 0.000 description 1
- 229960005066 trisodium edetate Drugs 0.000 description 1
- SOBHUZYZLFQYFK-UHFFFAOYSA-K trisodium;hydroxy-[[phosphonatomethyl(phosphonomethyl)amino]methyl]phosphinate Chemical compound [Na+].[Na+].[Na+].OP(O)(=O)CN(CP(O)([O-])=O)CP([O-])([O-])=O SOBHUZYZLFQYFK-UHFFFAOYSA-K 0.000 description 1
- 229910052720 vanadium Inorganic materials 0.000 description 1
- 239000003039 volatile agent Substances 0.000 description 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Description
第一の実施形態にかかる微生物の代謝経路制御方法は、ピルビン酸から代謝産物を産生する微生物を培養液に投入し、培養液と電解液とをイオン交換膜を介して接触させ、培養液に作用電極と参照電極を接触させ、電解液に対電極を接触させ、作用電極と対電極と参照電極とを定電位設定装置に結線し、作用電極の電位を3電極方式で制御して、微生物の代謝経路を制御するようにしている。
図3に示す電気培養装置1は、密閉構造の容器20を培養槽7とし、容器20に収容可能な密閉構造の小容器21を対電極槽8とし、小容器21は少なくとも一部にイオン交換膜6を備えると共にガス(対電極10から発生するガス)を容器20の外に排出するガス排出管22を備えるものとしている。また、対電極10と定電位設定装置12を結線する配線は、ガス排出管22の中を通過させている。尚、図3に示す電気培養装置1では、対電極10と定電位設定装置12を結線する配線は、ガス排出管22の中を通過させているが、必ずしもこの構成には限定されず、配線をガス排出管22を通さずに定電位設定装置12と結線するようにしてもよい。
図4に示す電気培養装置1は、上方が開放されている容器23をイオン交換膜6で仕切ることにより開放された二つの槽が形成され、培養槽7としての一方の槽の上方開放部がガス不透過膜またはガス不透過部材24により塞がれているものとしている。つまり、図4に示す電気培養装置1は、イオン交換膜6による培養槽7と対電極槽8の仕切り構成以外は、実質的には図3と同一の構成であり、図3に示す電気培養装置を用いた場合と同様の効果が奏され得る。
図5に示す電気培養装置1は、収容される液体の液面よりも下部に開口部を備える二つの容器25aと25bがイオン交換膜6を介して開口部で連結されてU字型の容器25が形成され、一方の容器25aを密閉構造として培養槽7とし、他方の容器25bを開放して対電極槽8としている。この場合、培養液4と電解液4aがイオン交換膜6を介して接触すると共に、培養槽7の培養液4の液面よりも上部の空間と対電極槽8の電解液4aの液面よりも上部の空間とが容器25自体のU字型構造によって隔てて配置される。つまり、図5に示す電気培養装置1は、容器25の形状以外の構成については、実質的には図4と同一の構成である。したがって、図4(さらには図3)に示す電気培養装置を用いた場合と同様の効果が奏され得る。
図6に示す電気培養装置1は、収容される液体の液面よりも下部に開口部を備える二つの容器26aと26bがイオン交換膜6を介して開口部で連結されてH字型の容器26が形成され、一方の容器26aを密閉構造として培養槽7とし、他方の容器26bを開放して対電極槽8としている。この場合にも、培養液4と電解液4aがイオン交換膜6を介して接触すると共に、培養槽7の培養液4の液面よりも上部の空間と対電極槽8の電解液4aの液面よりも上部の空間とが容器26自体のH字型構造によって隔てて配置される。つまり、図6に示す電気培養装置1は、容器25の形状以外の構成については、実質的には図4と同一の構成である。したがって、図4(さらには図3)に示す電気培養装置を用いた場合と同様の効果が奏され得る。
第二の実施形態にかかる微生物の代謝経路制御方法は、ピルビン酸から代謝産物を産生する微生物を培養液に投入し、培養液に作用電極と参照電極を接触させ、培養液と対電極とをイオン交換膜を介して接触させ、作用電極と対電極と参照電極とを定電位設定装置に結線し、作用電極の電位を3電極方式で制御して、微生物の代謝経路を制御するようにしている。つまり、第一の実施形態における微生物の代謝経路制御方法とは、電解液を用いることなく対電極を直接イオン交換膜に接触させている点のみが異なっている。
(1)実施例1において使用した電気培養装置
実施例1では、図6に示す電気培養装置を用いて実験を行った。250mL容の2つのガラスバイアル瓶(Duran製)のうちの一方を培養槽7とし、他方を対電極槽26bとし、下部開口部において陽イオン交換膜(ナフィオンK、デュポン製)106を介して2つのバイアル瓶を接続し、H字型の容器26とした。培養槽7には蓋をし、蓋の上面にはシリコーンゴム栓を設けて、配線や電極を通した際の密閉製を確保した。
実施例2では、図18に示す電気培養装置を用いて実験を行った。培養槽7としての容器20は250mL容のガラスバイアル瓶(Duran製)とした。培養液4は容器20の八分目程度まで入れた。容器20には蓋30をした。蓋30の上面30aにはシリコーンゴム栓を設けて、配線や電極、管を通した際の容器20の密閉性を確保した。また、シリコーンゴム栓を設けることにより注射針の突き刺しを可能とし、且つ注射針の差し込みにより生じた孔が注射針を抜いた際に塞がるようにした。
ピルビン酸を乳酸に還元する還元経路とピルビン酸をアセチル補酵素Aに酸化する酸化経路とを代謝経路として有する微生物として、超好熱性の加水分解菌であるサーモトガ マリティマ DSM3109(Thermotoga maritima DSM3109、以下、サーモトガと呼ぶ)を用いて、培養試験を実施した。
[培地組成]
スターチ :1.0g
KH2PO4 :0.5g
NaCl :20g
NiCl2・H2O :0.002g
イーストエクストラクト:0.5g
人工海水 :250mL
微量元素溶液 :10mL
pH6.5
[微量元素溶液の組成(L−1)]
ニトリロ三酢酸 1.5g
MgSO4・7H2O 3g
MnSO4・2H2O 0.5g
NaCl 1.0g
FeSO4・7H2O 0.1g
CoSO4・7H2O 0.18g
CaCl2・2H2O 0.1g
ZnSO4・7H2O 0.18g
CuSO4・5H2O 0.01g
H3BO3 0.01g
Na2MoO4・2H2O 0.01g
NiCl2・6H2O 0.025g
Na2SeO3・5H2O 0.8mg
Na2WO4 0.8mg
KAl(SO4)2・12H2O 4mg
水 1L
[人工海水]
MgSO4・7H2O 7g
MgCl2・6H2O 5.5g
NaCl 27.7g
CaCl2・2H2O 2.25g
KCl 0.65g
NaBr 0.1g
H3BO3 30mg
SrCl2・6H2O 15mg
クエン酸 10mg
KI 0.05mg
水 1L
ピルビン酸を乳酸に還元する還元経路とピルビン酸をアセチル補酵素Aに酸化する酸化経路とを代謝経路として有する微生物として、大腸菌を用いて培養試験を実施した。
[培養液の組成(L−1)]
・グルコース: 5.0g
・KH2PO4: 4.2g
・NH4Cl: 0.6g
・MgCl2・7H2O: 0.08g
・カザミノ酸: 5.0g
・微量元素溶液: 1mL
[微量元素溶液の組成(L−1)]
・FeSO4・7H2O: 0.2g
・CuSO4・5H2O: 1.0g
・NaMoO4・2H2O: 0.034g
・CaCl2・2H2O: 0.015g
・Na2SeO4: 0.5g
Claims (3)
- ピルビン酸を乳酸に還元する還元経路とピルビン酸をアセチル補酵素Aに酸化する酸化経路とを代謝経路として有する微生物を含む培養環境に電極を接触させ、前記電極の電位Xを銀・塩化銀電極電位基準でX≦−0.6(V)に制御しながら培養することにより、前記還元経路における還元反応を促進させると共に前記酸化経路における酸化反応を減退させることを特徴とする微生物の代謝経路制御方法。
- ピルビン酸を乳酸に還元する還元経路とピルビン酸をアセチル補酵素Aに酸化する酸化経路とを代謝経路として有する微生物を含む培養環境に電極を接触させ、前記電極の電位Xを銀・塩化銀電極電位基準でX≧−0.3(V)に制御しながら培養することにより、前記還元経路における還元反応を減退させると共に前記酸化経路における酸化反応を促進させることを特徴とする微生物の代謝経路制御方法。
- 前記微生物が大腸菌又は加水分解菌である、請求項1又は2に記載の方法。
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2011067404A JP5832766B2 (ja) | 2011-03-25 | 2011-03-25 | 微生物の代謝経路制御方法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2011067404A JP5832766B2 (ja) | 2011-03-25 | 2011-03-25 | 微生物の代謝経路制御方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
JP2012200200A JP2012200200A (ja) | 2012-10-22 |
JP5832766B2 true JP5832766B2 (ja) | 2015-12-16 |
Family
ID=47181819
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2011067404A Active JP5832766B2 (ja) | 2011-03-25 | 2011-03-25 | 微生物の代謝経路制御方法 |
Country Status (1)
Country | Link |
---|---|
JP (1) | JP5832766B2 (ja) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP6399536B2 (ja) * | 2014-03-03 | 2018-10-03 | 一般財団法人電力中央研究所 | 微生物の遺伝子発現制御方法 |
CN116751732B (zh) * | 2023-07-11 | 2024-05-24 | 内蒙古农业大学 | 一种提升干酪乳酪杆菌活细胞数量的培养方法 |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP5123503B2 (ja) * | 2006-09-04 | 2013-01-23 | 一般財団法人電力中央研究所 | 微生物の活性制御方法 |
JP5416355B2 (ja) * | 2008-02-01 | 2014-02-12 | 一般財団法人電力中央研究所 | 硫酸還元細菌の培養方法 |
-
2011
- 2011-03-25 JP JP2011067404A patent/JP5832766B2/ja active Active
Also Published As
Publication number | Publication date |
---|---|
JP2012200200A (ja) | 2012-10-22 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Bajracharya et al. | Carbon dioxide reduction by mixed and pure cultures in microbial electrosynthesis using an assembly of graphite felt and stainless steel as a cathode | |
Bajracharya et al. | Long-term operation of microbial electrosynthesis cell reducing CO2 to multi-carbon chemicals with a mixed culture avoiding methanogenesis | |
Jourdin et al. | Autotrophic hydrogen-producing biofilm growth sustained by a cathode as the sole electron and energy source | |
Jeremiasse et al. | Microbial electrolysis cell with a microbial biocathode | |
Blanchet et al. | Importance of the hydrogen route in up-scaling electrosynthesis for microbial CO 2 reduction | |
JP5563774B2 (ja) | 電気培養装置 | |
Villano et al. | Bioelectrochemical hydrogen production with hydrogenophilic dechlorinating bacteria as electrocatalytic agents | |
Cheng et al. | Ammonia recycling enables sustainable operation of bioelectrochemical systems | |
Jafary et al. | A comprehensive study on development of a biocathode for cleaner production of hydrogen in a microbial electrolysis cell | |
Bian et al. | Resistance assessment of microbial electrosynthesis for biochemical production to changes in delivery methods and CO2 flow rates | |
Sleutels et al. | Steady-state performance and chemical efficiency of Microbial Electrolysis Cells | |
CN104328046A (zh) | 微生物电化学系统还原二氧化碳产乙酸的装置与方法 | |
Zeppilli et al. | Anodic vs cathodic potentiostatic control of a methane producing microbial electrolysis cell aimed at biogas upgrading | |
US10494596B2 (en) | Enhanced microbial electrosynthesis by using co-cultures | |
CN103881905A (zh) | 一种嵌入式生物电合成系统及方法 | |
Zhang et al. | Enhancing methane oxidation in a bioelectrochemical membrane reactor using a soluble electron mediator | |
JP5832766B2 (ja) | 微生物の代謝経路制御方法 | |
Pozo et al. | Selective cathodic microbial biofilm retention allows a high current-to-sulfide efficiency in sulfate-reducing microbial electrolysis cells | |
JP5700620B2 (ja) | 水素資化性メタン菌のメタン生成活性制御方法 | |
JP5840371B2 (ja) | 微生物を利用した水素製造方法及び装置 | |
JP5323543B2 (ja) | 電気培養方法及び電気培養装置 | |
JP5372826B2 (ja) | 微生物群担持担体の作製方法並びに微生物を利用した物質処理方法及び物質生産方法 | |
Rovira-Alsina et al. | Transition roadmap for thermophilic carbon dioxide microbial electrosynthesis: Testing with real exhaust gases and operational control for a scalable design | |
JP5745292B2 (ja) | 発酵処理装置 | |
JP5875770B2 (ja) | 硝酸呼吸能を有する微生物の培養方法 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20140129 |
|
A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20150414 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20150525 |
|
TRDD | Decision of grant or rejection written | ||
A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20151027 |
|
A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20151028 |
|
R150 | Certificate of patent or registration of utility model |
Ref document number: 5832766 Country of ref document: JP Free format text: JAPANESE INTERMEDIATE CODE: R150 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |