JP2010155786A - Recalcification promotor for tooth and recalcification method - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a recalcification promotor for tooth or recalcification method which expresses a desired effect in a shorter period. <P>SOLUTION: The recalcification promotor for tooth contains potassium phosphate or sodium phosphate and a calcium salt. <P>COPYRIGHT: (C)2010,JPO&INPIT

Description

  The present invention relates to a tooth remineralizing agent and a remineralizing method.

  As this type of tooth remineralizing agent, a tooth remineralizing agent containing amorphized hydroxyapatite (so-called amorphous hydroxyapatite) is known (see Patent Document 1). This patent document reports that this amorphized hydroxyapatite can promote tooth remineralization (reconstruction of tooth enamel) relatively favorably than crystalline hydroxyapatite. is there.

In the known technique, amorphized hydroxyapatite is prepared by keeping a mixed solution of calcium salt and phosphate at room temperature for 2 to 3 days (see paragraph [0019] of the specification of Patent Document 1). Then, by immersing human extracted teeth in this amorphized hydroxyapatite for 24 hours, remineralization of the teeth can be promoted (see paragraph [0047] of the specification of Patent Document 1).
JP 2008-260702 A

However, the above-described known technique has a problem that it takes time to prepare an amorphized hydroxyapatite (the production efficiency is extremely low) (see paragraph [0009] of the specification of Patent Document 1). Furthermore, the tooth remineralizing agent of the publicly known technique is unexpectedly slow in its effect, and soaking for a long period of time is necessary to achieve the desired remineralization action.
Thus, the present invention has been devised in view of the above points, and the problem to be solved by the present invention is to provide a tooth remineralizing agent or remineralizing method that achieves a desired effect in a shorter period of time. It is to provide.

As a result of intensive studies to solve the above problems, the present inventors have found that calcium phosphates such as hydroxyapatite can be formed in a short period of time by combining a plurality of phosphates, and the present invention has been completed. It was.
That is, as means for solving the above problems, the tooth remineralizing agent of the first invention according to the present invention contains potassium phosphate, sodium phosphate and calcium salt (simulated body fluid component).
According to the present invention, by using a combination of these potassium phosphate and sodium phosphate (more preferably potassium dihydrogen phosphate and disodium hydrogen phosphate), calcium phosphate (for example, hydroxyapatite) can be used in a relatively short period of time. Can be formed.

The method for remineralizing a tooth according to the second aspect of the present invention includes a step of applying one of potassium phosphate, sodium phosphate, and calcium salt to a tooth, and then applying the other to the tooth.
According to the present invention, recalcification of teeth can be favorably promoted by forming calcium phosphate (for example, hydroxyapatite) on the teeth.

The tooth remineralization method according to the third aspect of the present invention is the tooth remineralization method according to the second aspect of the present invention, and includes a step of imparting a bleaching promoting component such as a strong base or peroxide to the tooth.
According to the remineralization method of the present invention, it is possible to promote the remineralization of teeth and achieve a suitable bleaching action.

  According to the first invention of the present invention, it is possible to provide a tooth remineralizing agent that achieves a desired effect (formation of calcium phosphate) in a shorter period of time. Further, according to the second invention, it is possible to provide a method for remineralizing a tooth that achieves a desired effect (facilitation of remineralization) in a shorter period of time. Moreover, according to the remineralization method of 3rd invention, both remineralization of a tooth and bleaching can be show | played.

Hereinafter, the best mode for carrying out the present invention will be described.
The tooth remineralizing agent according to the present embodiment is exclusively for promoting the remineralization of the tooth, and this type of tooth remineralizing agent has a desired effect in a short period of time as much as possible. desirable.
Therefore, in this example, the desired effects were achieved in the shortest possible time by using various components (pseudo body fluid component, bleaching acceleration component, photocatalyst) described later. Hereinafter, each configuration will be described in detail.

[Tooth]
The tooth of the present embodiment is a tooth (an unmyelinated tooth, a myelinated tooth) of a living body (an organism such as human beings, animals, birds, fish, and crustaceans). Here, the teeth are also sites that are easily colored due to exogenous or intrinsic causes. Examples of exogenous coloring causes include deposition of colored substances (cigarettes, tea astringents, etc.), pigment-producing bacteria, discoloration of restorations (mainly composite resins), and coloring with metal salts (mainly amalgam, silver nitrate, silver ammonia). . Examples of intrinsic coloring causes include aging, chemical substances and drugs (fluorine, tetracycline, etc.), metabolic abnormalities, genetic diseases, and tooth injury.

[Various components of remineralizing agent]
(Pseudo body fluid component)
The simulated body fluid component contains a calcium salt, potassium phosphate, and sodium phosphate (buffer component). This simulated body fluid component can efficiently promote the formation of calcium phosphate such as hydroxyapatite (chemical formula: Ca ₁₀ (PO ₄ ) ₆ (OH) ₂ ).
Here, the calcium salt is preferably a calcium salt that can be dissolved in an aqueous solvent, and examples thereof include calcium chloride, calcium sulfate, calcium lactate, and calcium acetate.
Among potassium phosphates, potassium dihydrogen phosphate (KH ₂ PO ₄ ) is preferable because it is easily available and inexpensive. And Among sodium phosphate, disodium hydrogen phosphate (Na 2 _{HPO 4)} is preferable because a easy also inexpensive availability.

And the content of calcium salt, sodium phosphate and potassium phosphate (phosphate) is not particularly limited as long as it can form calcium phosphate.
Preferably, the calcium salt is 0.125% to 0.75% by weight with respect to the total weight of the remineralizing agent. If the calcium salt content is less than 0.125%, the calcium phosphate forming action may be reduced. Further, the content of calcium salt may be more than 0.75%, but the cost is increased because an extreme increase in calcium phosphate forming action is not observed.

Further, sodium phosphate is 0.03% to 0.345% by weight with respect to the total weight of the remineralizing agent. If the content of sodium phosphate is less than 0.03%, there is a risk of reducing the calcium phosphate forming action. Further, the content of sodium phosphate may be more than 0.345%, but the cost is increased because an extreme increase in calcium phosphate forming action is not observed.
And potassium phosphate is 0.005%-0.06% by weight ratio with respect to the remineralization agent total weight. If the content of potassium phosphate is less than 0.005%, the formation action of calcium phosphate may be reduced. Further, the content of potassium phosphate may be more than 0.06%, but the cost is increased because an extreme increase in calcium phosphate forming action is not observed.

(Content ratio of calcium salt and phosphate)
It is desirable that the content ratio of the calcium salt and phosphate (sodium phosphate and potassium phosphate) can form, for example, hydroxyapatite efficiently.
Here, as described above, hydroxyapatite is represented by the stoichiometric composition (Ca / P molar ratio is 1.67) represented by the chemical formula: Ca ₁₀ (PO ₄ ) ₆ (OH) ₂ . Hydroxyapatite is known to exhibit the properties of hydroxyapatite and have an apatite structure even in a non-stoichiometric case where the Ca / P molar ratio is not 1.67.
For this reason, it is preferable that the content ratio of the calcium salt and the phosphate is appropriately adjusted so that the Ca / P molar ratio is in the vicinity of 1.67 (range of 1.4 to 1.9).

(Bleaching acceleration component)
Examples of the bleach accelerating component include peroxides, strong bases, weak bases, strong acids, and weak acids.
Among them, it is desirable to use a strong base. For example, a base having an acid pKa of 15 or more is preferable. Examples of the strong base include organic strong bases (alkyl metal, metal alkoxide, acetylide, organic metal amide, etc.) and inorganic strong bases (metal hydride, metal hydroxide, inorganic metal amide, etc.). . These strong bases may be used alone or in combination.
Among them, potassium hydroxide (KOH), sodium hydroxide (NaOH), calcium hydroxide (Ca _(OH) 2), metal hydroxides such as barium hydroxide (Ba _{(OH) 2)} is relatively available Since it is easy, it can be used suitably as a bleaching acceleration component of the present Example. Furthermore, since potassium hydroxide and sodium hydroxide have an excellent bleaching action, they can be particularly preferably used as a bleaching acceleration component in this example.
By using a strong base having a lower structure corrosivity than a peroxide, the bleaching action can be improved without adversely affecting the teeth.

The content of the strong base is not particularly limited as long as the bleaching promotion effect is exerted without adversely affecting the teeth as much as possible. For example, the content of the strong base is preferably 0.01% or more by weight with respect to the total weight of the tooth remineralizing agent. If the content of the strong base is less than 0.01%, there is a possibility that the desired bleaching acceleration effect may not be achieved.
Moreover, it is desirable that the upper limit value of the strong base is 1.0% or less by weight with respect to the total weight of the tooth remineralizing agent, and more preferably less than 1.0%. By setting the content of strong base to 0.2% to 0.5% (relatively small amount), a suitable bleaching action can be achieved without adversely affecting the living body as much as possible.

(photocatalyst)
A photocatalyst is a particle that radicalizes oxygen in the atmosphere using light or the like (has a photocatalytic effect). The particle size of the photocatalyst is preferably 1 nm to 200 nm.
Examples of the photocatalyst include metal oxide semiconductors (including transition metal oxides), sulfide semiconductors, metal-supported semiconductors in which a metal such as platinum is supported on the semiconductors, and metal complex particles.
Among these, particles of titanium dioxide (TiO ₂ , an example of a metal oxide semiconductor) have excellent photocatalytic activity and are easily available, and thus are preferably used as the photocatalyst of this example. Titanium dioxide may be any of anatase type, rutile type, and flukeite type.

  The content of the photocatalyst is not particularly limited as long as it exhibits a photocatalytic action, but it is preferably 0.001% to 0.1% by weight with respect to the total weight of the tooth remineralizing agent. If the content of the photocatalyst is less than 0.001%, the desired photocatalytic action may not be achieved. On the other hand, when the content of the photocatalyst is more than 0.1%, the cost is increased and the photocatalytic action may be slightly lowered due to deterioration of light transmittance.

(Other ingredients)
In addition to the above components, various components can be added to the remineralizing agent of this example. For example, when the remineralizing agent is used as acidic as described later, citric acid or trisodium citrate (buffering agent) is appropriately added to the remineralizing agent to adjust its pH to 5 or less. it can. Further, by adding sodium chloride (sodium salt) or potassium chloride (potassium salt) to the remineralizing agent to bring it close to (or the same as) the salinity concentration of the body fluid, the recalcifying agent can be easily adapted to the living body.

[Tooth remineralization method]
In the present embodiment, there is a first step of applying a simulated body fluid component to a tooth, and a second step of applying a bleach accelerating component to the tooth. In the second step, light (ultraviolet rays) and ultrasonic waves described later are irradiated. Hereinafter, each process is explained in full detail.

(First step)
In the first step, after applying potassium phosphate and sodium phosphate (phosphate) to a tooth, a calcium salt is applied to the tooth. That is, in this embodiment, after phosphate (minus ion) is applied to the tooth, calcium (plus ion) is mixed on the tooth to bind calcium and phosphorus on the tooth.
Thus, by preparing the pseudo body fluid component on the tooth (forming calcium phosphate on the tooth), remineralization of the tooth can be favorably promoted. Furthermore, the simulated body fluid component does not inhibit the bleaching action in the second step described later as much as possible.

And although processing time is not specifically limited, For example, even if it is 30 minutes-60 minutes (relatively short period) after providing a calcium salt to a tooth, the remineralization effect | action of a tooth can be show | played. In addition, by preliminarily keeping the phosphate and calcium salt at around body temperature (30 ° C. to 50 ° C.), formation of calcium phosphate can be preferably promoted in about 1 minute to 5 minutes.
In addition, formation confirmation of calcium phosphate can be discriminate | determined, for example with a predetermined measuring instrument or visual observation (whether the tooth became cloudy).

The simulated body fluid component is preferably in a neutral range (pH 6 to pH 8). Thus, the remineralization of a tooth can be favorably promoted by accelerating the formation of apatite under neutral range conditions close to a living body.
For example, by setting the content ratio of potassium phosphate (A) and sodium phosphate (B) to A: B = 1: 5 to 1:10 by weight ratio, the pseudo body fluid component on the tooth is made neutral. be able to.

(Second step)
And in a 2nd process, after giving a bleaching acceleration | stimulation component and a photocatalyst with respect to the tooth after a 1st process, the below-mentioned light or an ultrasonic wave is irradiated.
In addition, as a method of making a bleaching acceleration component adhere to a tooth, various methods, such as application | coating and spraying of a bleaching acceleration component, can be taken. Further, the bleach accelerating component may be attached to the tooth once, or may be attached a plurality of times. In the case where the bleaching acceleration component is attached a plurality of times, the bleaching acceleration component is usually attached to the tooth every about 1 to 20 minutes, and the interval and frequency are appropriately set according to the coloring state of the tooth. be able to.

(Light irradiation)
The light (ultraviolet light) of the present embodiment may be light having a wavelength that can cause the photocatalyst to act on the photocatalyst (wavelength capable of photoexciting titanium dioxide), and is light having a less adverse effect on the living body. It is desirable.
For example, when using titanium dioxide as a photocatalyst, typically, light having a wavelength of 300 to 500 nm is used (the wavelength for photoexciting rutile titanium dioxide is around 415 nm, and the wavelength for photoexciting anatase titanium dioxide is 380 nm or less. Is). In addition, when the light exceeding wavelength 500nm is irradiated for a long time, the temperature rise of a tooth may become large.
Moreover, the ultraviolet intensity of light is suitably changed according to the coloring state of a tooth. Typically, it is desirable that the ultraviolet intensity is stronger than sunlight (light having an ultraviolet intensity of about 1 mW / cm ² ).

Examples of the light source include black light, exothermic lamp, fluorescent lamp, halogen lamp, metal halide lamp, xenon lamp, mercury lamp, UV lamp, LED (light emitting diode), and semiconductor laser.
Then, after the unnecessary wavelength is cut by the optical filter, the tooth to which the bleaching promoting component is applied is irradiated with the light from the light source. The time of light irradiation can be appropriately adjusted according to the wavelength of light and the coloring state of the teeth.

(Ultrasonic irradiation)
Moreover, the ultrasonic wave of a present Example should just be an ultrasonic wave which has the frequency (frequency which can generate cavitation in a remineralization agent) which can produce a photocatalytic action to a photocatalyst, and has a little bad influence with respect to a biological body It is desirable that
Then, ultrasonic waves are applied to the teeth to which the bleaching promoting component is applied. The time and output (power) of ultrasonic irradiation can be appropriately adjusted according to the frequency of the ultrasonic waves and the coloring state of the teeth.

The above-described ultrasonic waves are preferably ultrasonic waves having a frequency of about 20 kHz to 50 kHz, and more preferably ultrasonic waves having a frequency of about 25 kHz to 35 kHz. Here, in the case of ultrasonic waves of less than 20 kHz, there is a possibility that bad influences such as the teeth being damaged by cavitation and the occurrence of operation noise may occur.
The frequency of the ultrasonic wave may exceed 50 kHz, but no extreme increase in photocatalytic action is observed. For this reason, it is desirable that the frequency of the ultrasonic wave be 50 kHz or less in consideration of adverse effects on the living body.

And when irradiating a tooth | gear with a ultrasonic wave, even if a remineralizer is acidic (when less than pH 7) or alkaline (when it exceeds pH 7), there exists a suitable bleaching effect | action.
Here, tooth whitening is often performed under alkaline conditions in consideration of the fact that teeth are resistant to alkali. And the tooth | gear whitened under alkaline conditions will be in the bleaching state with a transparent feeling.
In addition, teeth that have been whitened under acidic conditions have some unevenness on the surface due to the acid, so that the apparent whiteness increases. Therefore, when white teeth are desired, whitening is preferably performed under acidic conditions. And in the remineralization method of a present Example, tooth whitening can be performed suitably also in acidic conditions by the synergistic effect of the said various components and an ultrasonic wave.

As described above, according to the tooth remineralizing agent of this example, calcium phosphate can be formed in a shorter period of time by a plurality of phosphates. Further, according to the method for remineralizing a tooth according to the present embodiment, it is possible to favorably promote remineralization of the tooth by forming calcium phosphate on the tooth. Moreover, in a present Example, bleaching (whitening) of a tooth can be performed with remineralization of a tooth by using a bleaching acceleration component together.
And in a present Example, the variation of tooth whitening can be increased by the synergistic effect of various components and an ultrasonic wave.

[Test example]
Hereinafter, although this Embodiment is demonstrated based on a test example, this invention is not limited to a test example.
Example 1
For 100 ml of purified water, 0.25 g of potassium hydroxide, simulated body fluid component (0.5 g of calcium chloride, 1.15 g of disodium hydrogen phosphate, 0.05 g of potassium dihydrogen phosphate, both of which are Wako Co., Ltd. The remineralizing agent (pH 10 or more) of Example 1 was prepared.
(Example 2)
A remineralizing agent (pH 10 or more) of Example 2 was prepared by adding 0.25 g of potassium hydroxide, 0.03 g of titanium dioxide, and the above-mentioned simulated body fluid component to 100 ml of purified water.
(Example 3)
To the remineralizing agent of Example 1, 2 g of citric acid and 3 g of trisodium citrate were added to prepare the remineralizing agent of Example 3 (pH 5 or lower).
Example 4
2 g of citric acid and 3 g of trisodium citrate were added to the remineralizing agent of Example 2 to prepare the remineralizing agent of Example 4 (pH 5 or lower).

(Reference Example 1)
To 100 ml of purified water, 0.25 g of potassium hydroxide (Kanto Chemical Co., Inc.) was added to prepare the remineralizing agent (pH 10 or more) of Reference Example 1.
(Reference Example 2)
To 100 ml of purified water, 0.25 g of potassium hydroxide and 0.03 g of titanium dioxide (Aerosil) were added to prepare a remineralizing agent (pH 10 or more) of Reference Example 2.
(Reference Example 3)

2 g of citric acid (Kanto Chemical Co., Inc.) and 3 g of trisodium citrate (Kanto Chemical Co., Ltd.) were added to the remineralizing agent of Reference Example 1 to prepare a remineralizing agent (pH 5 or less) of Reference Example 3.
(Reference Example 4)
2 g of citric acid and 3 g of trisodium citrate were added to the remineralizing agent of Reference Example 2 to prepare the remineralizing agent of Reference Example 4 (pH 5 or lower).

(Reference Example 5)
A remineralizing agent of Reference Example 5 was prepared by adding 10% of oxidol (Kenei Pharmaceutical) to 100 ml of purified water.
(Reference Example 6)
The remineralizing agent of Reference Example 6 was prepared by adding 0.03 g of titanium dioxide to the remineralizing agent of Reference Example 5.

(Test method)
1-a. Remineralization confirmation test (1)
As a test sample, a human extracted tooth (extracted tooth without dental preservation or prosthetic remineralization) was used (see comparative sample (S0), FIG. 1 (a), and FIG. 2 (a)). In addition, the dirt and deposits on the enamel surface of the extracted tooth were removed in advance.
The phosphate buffer was prepared by adding 4 g of dipotassium hydrogen phosphate and 23 g of disodium hydrogen phosphate to 2000 ml of purified water (pH 6-8). The calcium chloride solution was prepared by adding 10 g of calcium chloride to 100 ml of purified water. Each of these solutions was stirred (always) at 37 ° C to 39 ° C. Each reagent used was one manufactured by Wako.

Then, the extracted tooth of the test sample was put into 1750 ml of purified water, 200 ml of phosphate buffer was added, and then 50 ml of calcium chloride solution was added. Then, the extracted tooth of the test sample was allowed to stand for 1 minute, and then washed with distilled water was used as the extracted tooth (S1) of Sample 1 (see FIGS. 1B and 2B).
The extracted tooth of the test sample was allowed to stand for 5 minutes and then washed with distilled water to obtain the extracted tooth (S2) of Sample 2 (see FIGS. 1 (c) and 2 (c)).
The extracted tooth of the test sample was allowed to stand for 10 minutes and then washed with distilled water to obtain the extracted tooth (S3) of Sample 2 (see FIGS. 1 (d) and 2 (d)).
The extracted tooth of the test sample was allowed to stand for 30 minutes and then washed with distilled water to obtain the extracted tooth (S4) of Sample 2 (see FIGS. 1 (e) and 2 (e)).
The extracted tooth of the test sample was allowed to stand for 60 minutes and then washed with distilled water to obtain the extracted tooth (S5) of Sample 2 (see FIGS. 1 (f) and 2 (f)).
And the surface (after drying) of each sample was image | photographed by the magnification of 400 times and 1000 times, and the presence or absence of remineralization was confirmed (refer FIG.1 and FIG.2). A desktop scanning electron microscope (JEOL Ltd., trade name: NeoScope JCM-5000) was used for photographing.

6-b. Remineralization confirmation test (2)
In this test, 1 g of purified water was charged with 5 g of potassium hydroxide and 0.6 g of titanium dioxide. The extracted tooth of the test sample was put into this purified water, 200 ml of phosphate buffer was added, and then 50 ml of calcium chloride solution was added.
Then, the extracted tooth of the test sample was allowed to stand for 10 minutes, and then washed with distilled water was used as the extracted tooth (S6) of Sample 6 (see FIG. 3). Other test methods and imaging methods were the same as the remineralization confirmation test (1) described above.

2. Bleaching test using test paper A glossy film (Epson) dyed with an ethanol solution containing hematoporphyrin was used as a test paper. Each of the remineralizing agents of Example 1 and Example 2 was applied to a test paper and allowed to stand for 5 minutes. This operation was repeated 4 times.
The color of the test paper after the test was measured, and the degree of bleaching by the remineralizing agent of Example 1 and Example 2 was evaluated. As a colorimeter, a spectral colorimeter for dentistry (GC Corporation, trade name: VITA Easyshade (registered trademark)) was used. And the measured value (L value: brightness) by the spectrophotometer for dentistry was classified into the ranks of 1 to 5, and the degree of bleaching of the test paper was evaluated (see [Table 1] below).
The bleaching test of Reference Examples 1, 2, 5, and 6 was performed in the same procedure.

3. Bleaching test using eggshell Brown eggshell (production farm: Otomo Sangyo Co., Ltd., distributor: Chubu Feed Co., Ltd., trade name “Gamatamago”) was used as the test eggshell. Each of the remineralizing agents of Example 3 and Example 4 was applied to the test eggshell and allowed to stand for 5 minutes. This operation was repeated 4 times.
The test eggshell after the test was color-measured to evaluate the degree of bleaching by the remineralizing agents of Example 3 and Example 4, respectively. As the colorimeter, the above-mentioned spectrophotometer for dentistry was used. And the measured value by the spectrophotometer for dentistry was classified into the rank of 1-5, and the bleaching degree of the test eggshell was evaluated (refer to the following [Table 2]).
Then, bleaching tests of Reference Examples 3 and 4 were performed according to the same procedure.

4). Bleaching test by light A black light (Toshiba Corp., product number: EFD15BLB-T (100V, 15W)) was used as a light source. This black light was placed 3 cm away from the test paper.
And after apply | coating each remineralizing agent of Example 2 to a test paper, it was left to stand for 5 minutes, irradiating black light (wavelength: 352 nm). This operation was repeated 4 times. The test paper after the test was color-measured to evaluate the degree of bleaching.
The bleaching test of Example 4 was performed in the same procedure using the test eggshell.
And the bleaching test (test paper or test eggshell) of Reference Examples 2, 4, and 6 was performed in the same procedure.

5). Ultrasonic Bleaching Test An ultrasonic generator for dentistry (Satelec Acryon Group (France), trade name: Sprason P-MAX) was used as an ultrasonic wave generation source. An ultrasonic irradiation port (Slapson chip # 2) was attached to this dentistry ultrasonic generator, and this irradiation port was set 1 mm away from the test paper.
And after dripping each remineralizing agent of Example 2 on a test paper, each ultrasonic wave (frequency: 27-31 kHz, electric power: 1W) was irradiated for 5 minutes, adding each remineralizing agent suitably. The mouth was immersed in the remineralizing agent). This operation was repeated 4 times. The test paper after the test was color-measured to evaluate the degree of bleaching.
The bleaching test of Example 4 was performed in the same procedure using the test eggshell.
And the bleaching test (test paper or test eggshell) of Reference Examples 2, 4, and 6 was performed in the same procedure.

6). Bleaching test using ultrasonic waves and light The above-described black light was used as a light source. Moreover, the above-mentioned dentistry ultrasonic generator was used as an ultrasonic wave generation source.
And after apply | coating the remineralizing agent of Example 2 to a test paper, it was left to stand for 5 minutes, irradiating an ultrasonic wave and light. This operation was repeated 4 times. The test paper after the test was color-measured to evaluate the degree of bleaching.
The bleaching test of Example 4 was performed in the same procedure using the test eggshell.
And the bleaching test (test paper or test eggshell) of Reference Examples 2, 4, and 6 was performed in the same procedure.

(Test results and discussion)
The test results are shown in the following [Table 3] to [Table 7].

(Remineralization)
With reference to FIG.1 and FIG.2, it has confirmed that remineralization had arisen all in the extraction tooth | gear (S1) of the sample 1-the extraction tooth | gear (S5) of the sample 5 ((b)-of each figure). (See (f)). From this, it was found that according to the method of this example, the formation of calcium phosphate was promoted to exert an excellent remineralization action. In particular, since remineralization occurred in the extracted tooth (S1) of Sample 1, it was found that calcium phosphate (for example, hydroxyapatite) was formed even in a relatively short time (about 1 minute) in this method. It was also found that sufficient remineralization occurred in the extracted tooth (S4) of sample 4 and the extracted tooth (S5) of sample 5. From this, according to this method, it turned out that sufficient remineralization of a tooth | gear can be achieved by the process for 30 minutes-60 minutes.
Furthermore, the remineralizing agents of Example 3 and Example 4 exhibited an excellent bleaching action even in the presence of a simulated body fluid component. This indicates that the simulated body fluid component does not adversely affect the tooth bleaching action.
Summing up these results, according to the remineralizing agent and the remineralization method of the present example, while exhibiting a suitable bleaching action, the remineralization of teeth (reconstruction of tooth enamel) is promoted. I understood.

And with reference to FIG. 3, it has confirmed that remineralization had arisen also in the extraction tooth | gear (S6) of the sample 6. FIG. From this, it was found that the bleach-accelerating component (potassium hydroxide) and the photocatalyst (titanium dioxide) do not adversely affect the calcium phosphate forming action and the tooth remineralization action.
As a result, according to the remineralizing agent and the remineralization method of this example, it is possible to more reliably promote tooth remineralization (reconstruction of tooth enamel) while exhibiting a suitable bleaching action. It was supported.

(Strong base)
The remineralizing agents of Examples 1 and 2 and Reference Examples 1 and 2 were improved in the degree of bleaching compared to the remineralizing agents of Reference Examples 5 and 6 ([Table 3 ], [Table 5] and [Table 7]). In addition, the remineralizing agents of Example 3 and Example 4 and Reference Example 3 and Reference Example 4 exhibited bleaching action comparable to that of Reference Example 5 and Reference Example 6 even under acidic conditions ([Table 4 ] And [Table 6]).
From this, the remineralizing agents of Examples 1 to 3 and Reference Examples 1 to 4 exhibit excellent bleaching action (or bleaching action) with a relatively small amount of potassium hydroxide (strong base). To maintain).

(photocatalyst)
Moreover, the remineralization agent of Example 2 and Reference Example 2 was further improved in the degree of bleaching by irradiation with at least one of light (ultraviolet rays) and ultrasonic waves (see [Table 3] and [Table 5]).
From this, it can be seen that the remineralizing agents of Example 2 and Reference Example 2 have a more excellent bleaching action due to the synergistic effect of potassium hydroxide (strong base), titanium dioxide (photocatalyst) and light or ultrasonic waves. It was.

(Ultrasonic)
And the remineralizing agent of Example 4 and Reference Example 3 maintained a suitable bleaching action even under acidic conditions by ultrasonic irradiation (see [Table 4] and [Table 6]).
From this, it was found that the remineralizing agents of Example 4 and Reference Example 3 exhibited excellent bleaching action even under acidic conditions due to the synergistic effect of various components such as titanium dioxide (photocatalyst) and ultrasonic waves.

The tooth remineralization agent and the remineralization method according to the present embodiment are not limited to the above-described embodiments, and may take other various embodiments.
(1) The remineralizing agent of the present embodiment can be used alone, or can be used by being mixed with a favorite product such as a dentifrice, a mouthwash, or a chewing gum.
(2) In the first step of this example, an example in which potassium phosphate and sodium phosphate (phosphate) are applied to a tooth and then a calcium salt is applied to the tooth has been described.
Unlike this, potassium phosphate, sodium phosphate and calcium salt (simulated body fluid component) can be simultaneously applied to the teeth. Moreover, a simulated body fluid component and other components (a bleaching acceleration component or a photocatalyst) can be simultaneously applied to the teeth.
(3) Moreover, in the present Example, the 2nd process was performed after the 1st process. Unlike this, the second step may be performed before the first step.
(4) In the present embodiment, an example in which potassium phosphate and sodium phosphate (phosphate) are applied to a tooth and then a calcium salt is applied to the tooth has been described. Unlike this, after giving calcium salt to a tooth, you may give potassium phosphate and sodium phosphate (phosphate) to a tooth.

It is a 400 times enlarged view of the tooth surface for demonstrating remineralization. It is a 1000 times enlarged view of the tooth surface for demonstrating remineralization. (A) is a 400 times enlarged view of a tooth surface for explaining remineralization in another test, and (b) is a 1000 times enlarged tooth surface for explaining remineralization in another test. FIG.

Claims (3)

  A tooth remineralizing agent containing potassium phosphate, sodium phosphate and calcium salt.   A method for remineralizing a tooth comprising a step of applying one of potassium phosphate and sodium phosphate and a calcium salt to a tooth and then applying the other to the tooth. The method for remineralizing a tooth according to claim 2, comprising a step of imparting a bleaching promoting component such as a strong base or a peroxide to the tooth.