CN115024988A - Preparation and application of antibacterial enamel remineralization liquid - Google Patents
Preparation and application of antibacterial enamel remineralization liquid Download PDFInfo
- Publication number
- CN115024988A CN115024988A CN202210635367.5A CN202210635367A CN115024988A CN 115024988 A CN115024988 A CN 115024988A CN 202210635367 A CN202210635367 A CN 202210635367A CN 115024988 A CN115024988 A CN 115024988A
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- CN
- China
- Prior art keywords
- water
- oil
- calcium
- soluble
- zinc
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 210000003298 dental enamel Anatomy 0.000 title claims abstract description 93
- 230000000844 anti-bacterial effect Effects 0.000 title claims abstract description 32
- 238000002360 preparation method Methods 0.000 title claims abstract description 18
- 239000007788 liquid Substances 0.000 title claims abstract description 13
- 239000007764 o/w emulsion Substances 0.000 claims abstract description 52
- 239000004332 silver Substances 0.000 claims abstract description 50
- 229910052709 silver Inorganic materials 0.000 claims abstract description 50
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 claims abstract description 45
- 229910052725 zinc Inorganic materials 0.000 claims abstract description 45
- 239000011701 zinc Substances 0.000 claims abstract description 45
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 claims abstract description 39
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims abstract description 36
- 239000011575 calcium Substances 0.000 claims abstract description 36
- 229910052791 calcium Inorganic materials 0.000 claims abstract description 36
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims abstract description 36
- 229910019142 PO4 Inorganic materials 0.000 claims abstract description 33
- 239000010452 phosphate Substances 0.000 claims abstract description 33
- 150000003839 salts Chemical class 0.000 claims abstract description 25
- 230000000395 remineralizing effect Effects 0.000 claims abstract description 12
- 239000012530 fluid Substances 0.000 claims abstract description 10
- 238000011068 loading method Methods 0.000 claims abstract description 5
- 239000012071 phase Substances 0.000 claims description 44
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 40
- 229960005069 calcium Drugs 0.000 claims description 35
- 238000003756 stirring Methods 0.000 claims description 33
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 claims description 32
- 239000003921 oil Substances 0.000 claims description 28
- 235000019198 oils Nutrition 0.000 claims description 28
- -1 silver ions Chemical class 0.000 claims description 27
- 239000007762 w/o emulsion Substances 0.000 claims description 22
- 239000007864 aqueous solution Substances 0.000 claims description 21
- 239000003513 alkali Substances 0.000 claims description 20
- 239000002245 particle Substances 0.000 claims description 17
- 229910000019 calcium carbonate Inorganic materials 0.000 claims description 16
- 238000002156 mixing Methods 0.000 claims description 15
- 239000007787 solid Substances 0.000 claims description 15
- 238000000034 method Methods 0.000 claims description 14
- 230000001680 brushing effect Effects 0.000 claims description 13
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 12
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 12
- PTFCDOFLOPIGGS-UHFFFAOYSA-N Zinc dication Chemical compound [Zn+2] PTFCDOFLOPIGGS-UHFFFAOYSA-N 0.000 claims description 10
- 239000002585 base Substances 0.000 claims description 10
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 claims description 9
- 159000000007 calcium salts Chemical class 0.000 claims description 9
- 239000000606 toothpaste Substances 0.000 claims description 9
- ZCCIPPOKBCJFDN-UHFFFAOYSA-N calcium nitrate Chemical compound [Ca+2].[O-][N+]([O-])=O.[O-][N+]([O-])=O ZCCIPPOKBCJFDN-UHFFFAOYSA-N 0.000 claims description 8
- 229940034610 toothpaste Drugs 0.000 claims description 8
- JIAARYAFYJHUJI-UHFFFAOYSA-L zinc dichloride Chemical compound [Cl-].[Cl-].[Zn+2] JIAARYAFYJHUJI-UHFFFAOYSA-L 0.000 claims description 8
- UFTFJSFQGQCHQW-UHFFFAOYSA-N triformin Chemical compound O=COCC(OC=O)COC=O UFTFJSFQGQCHQW-UHFFFAOYSA-N 0.000 claims description 7
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 claims description 6
- WMFOQBRAJBCJND-UHFFFAOYSA-M Lithium hydroxide Chemical compound [Li+].[OH-] WMFOQBRAJBCJND-UHFFFAOYSA-M 0.000 claims description 6
- 229910001424 calcium ion Inorganic materials 0.000 claims description 6
- 239000000377 silicon dioxide Substances 0.000 claims description 6
- 239000000243 solution Substances 0.000 claims description 6
- 235000019483 Peanut oil Nutrition 0.000 claims description 5
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 5
- 239000000312 peanut oil Substances 0.000 claims description 5
- GGCZERPQGJTIQP-UHFFFAOYSA-N sodium;9,10-dioxoanthracene-2-sulfonic acid Chemical compound [Na+].C1=CC=C2C(=O)C3=CC(S(=O)(=O)O)=CC=C3C(=O)C2=C1 GGCZERPQGJTIQP-UHFFFAOYSA-N 0.000 claims description 5
- LWIHDJKSTIGBAC-UHFFFAOYSA-K tripotassium phosphate Chemical compound [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 claims description 5
- SNAAJJQQZSMGQD-UHFFFAOYSA-N aluminum magnesium Chemical compound [Mg].[Al] SNAAJJQQZSMGQD-UHFFFAOYSA-N 0.000 claims description 4
- AXCZMVOFGPJBDE-UHFFFAOYSA-L calcium dihydroxide Chemical compound [OH-].[OH-].[Ca+2] AXCZMVOFGPJBDE-UHFFFAOYSA-L 0.000 claims description 4
- 239000000920 calcium hydroxide Substances 0.000 claims description 4
- 229910001861 calcium hydroxide Inorganic materials 0.000 claims description 4
- MNNHAPBLZZVQHP-UHFFFAOYSA-N diammonium hydrogen phosphate Chemical compound [NH4+].[NH4+].OP([O-])([O-])=O MNNHAPBLZZVQHP-UHFFFAOYSA-N 0.000 claims description 4
- 239000000499 gel Substances 0.000 claims description 4
- 150000003378 silver Chemical class 0.000 claims description 4
- 239000011592 zinc chloride Substances 0.000 claims description 4
- 235000005074 zinc chloride Nutrition 0.000 claims description 4
- ONDPHDOFVYQSGI-UHFFFAOYSA-N zinc nitrate Chemical compound [Zn+2].[O-][N+]([O-])=O.[O-][N+]([O-])=O ONDPHDOFVYQSGI-UHFFFAOYSA-N 0.000 claims description 4
- RBWNDBNSJFCLBZ-UHFFFAOYSA-N 7-methyl-5,6,7,8-tetrahydro-3h-[1]benzothiolo[2,3-d]pyrimidine-4-thione Chemical compound N1=CNC(=S)C2=C1SC1=C2CCC(C)C1 RBWNDBNSJFCLBZ-UHFFFAOYSA-N 0.000 claims description 3
- 239000000120 Artificial Saliva Substances 0.000 claims description 3
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 3
- 229910002651 NO3 Inorganic materials 0.000 claims description 3
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 claims description 3
- 239000008346 aqueous phase Substances 0.000 claims description 3
- 239000001110 calcium chloride Substances 0.000 claims description 3
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 3
- 239000001506 calcium phosphate Substances 0.000 claims description 3
- 229910000389 calcium phosphate Inorganic materials 0.000 claims description 3
- 235000011010 calcium phosphates Nutrition 0.000 claims description 3
- 239000003795 chemical substances by application Substances 0.000 claims description 3
- 235000019797 dipotassium phosphate Nutrition 0.000 claims description 3
- 229910000396 dipotassium phosphate Inorganic materials 0.000 claims description 3
- 239000004519 grease Substances 0.000 claims description 3
- 239000002324 mouth wash Substances 0.000 claims description 3
- 229940051866 mouthwash Drugs 0.000 claims description 3
- SDLBJIZEEMKQKY-UHFFFAOYSA-M silver chlorate Chemical compound [Ag+].[O-]Cl(=O)=O SDLBJIZEEMKQKY-UHFFFAOYSA-M 0.000 claims description 3
- 229940096017 silver fluoride Drugs 0.000 claims description 3
- REYHXKZHIMGNSE-UHFFFAOYSA-M silver monofluoride Chemical compound [F-].[Ag+] REYHXKZHIMGNSE-UHFFFAOYSA-M 0.000 claims description 3
- 235000012424 soybean oil Nutrition 0.000 claims description 3
- 239000003549 soybean oil Substances 0.000 claims description 3
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 claims description 3
- 150000003751 zinc Chemical class 0.000 claims description 3
- 239000004254 Ammonium phosphate Substances 0.000 claims description 2
- 235000019484 Rapeseed oil Nutrition 0.000 claims description 2
- 235000018936 Vitellaria paradoxa Nutrition 0.000 claims description 2
- 241001135917 Vitellaria paradoxa Species 0.000 claims description 2
- 235000019498 Walnut oil Nutrition 0.000 claims description 2
- PNEYBMLMFCGWSK-UHFFFAOYSA-N aluminium oxide Inorganic materials [O-2].[O-2].[O-2].[Al+3].[Al+3] PNEYBMLMFCGWSK-UHFFFAOYSA-N 0.000 claims description 2
- LFVGISIMTYGQHF-UHFFFAOYSA-N ammonium dihydrogen phosphate Chemical compound [NH4+].OP(O)([O-])=O LFVGISIMTYGQHF-UHFFFAOYSA-N 0.000 claims description 2
- 229910000387 ammonium dihydrogen phosphate Inorganic materials 0.000 claims description 2
- 229910000148 ammonium phosphate Inorganic materials 0.000 claims description 2
- 235000019289 ammonium phosphates Nutrition 0.000 claims description 2
- VSGNNIFQASZAOI-UHFFFAOYSA-L calcium acetate Chemical compound [Ca+2].CC([O-])=O.CC([O-])=O VSGNNIFQASZAOI-UHFFFAOYSA-L 0.000 claims description 2
- 239000001639 calcium acetate Substances 0.000 claims description 2
- 235000011092 calcium acetate Nutrition 0.000 claims description 2
- 229960005147 calcium acetate Drugs 0.000 claims description 2
- NKWPZUCBCARRDP-UHFFFAOYSA-L calcium bicarbonate Chemical compound [Ca+2].OC([O-])=O.OC([O-])=O NKWPZUCBCARRDP-UHFFFAOYSA-L 0.000 claims description 2
- 229910000020 calcium bicarbonate Inorganic materials 0.000 claims description 2
- FNAQSUUGMSOBHW-UHFFFAOYSA-H calcium citrate Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O.[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O FNAQSUUGMSOBHW-UHFFFAOYSA-H 0.000 claims description 2
- 239000001354 calcium citrate Substances 0.000 claims description 2
- 229960004256 calcium citrate Drugs 0.000 claims description 2
- 239000004227 calcium gluconate Substances 0.000 claims description 2
- 235000013927 calcium gluconate Nutrition 0.000 claims description 2
- 229960004494 calcium gluconate Drugs 0.000 claims description 2
- MKJXYGKVIBWPFZ-UHFFFAOYSA-L calcium lactate Chemical compound [Ca+2].CC(O)C([O-])=O.CC(O)C([O-])=O MKJXYGKVIBWPFZ-UHFFFAOYSA-L 0.000 claims description 2
- 239000001527 calcium lactate Substances 0.000 claims description 2
- 235000011086 calcium lactate Nutrition 0.000 claims description 2
- 229960002401 calcium lactate Drugs 0.000 claims description 2
- BRPQOXSCLDDYGP-UHFFFAOYSA-N calcium oxide Chemical compound [O-2].[Ca+2] BRPQOXSCLDDYGP-UHFFFAOYSA-N 0.000 claims description 2
- 239000000292 calcium oxide Substances 0.000 claims description 2
- ODINCKMPIJJUCX-UHFFFAOYSA-N calcium oxide Inorganic materials [Ca]=O ODINCKMPIJJUCX-UHFFFAOYSA-N 0.000 claims description 2
- NEEHYRZPVYRGPP-UHFFFAOYSA-L calcium;2,3,4,5,6-pentahydroxyhexanoate Chemical compound [Ca+2].OCC(O)C(O)C(O)C(O)C([O-])=O.OCC(O)C(O)C(O)C(O)C([O-])=O NEEHYRZPVYRGPP-UHFFFAOYSA-L 0.000 claims description 2
- 229910000388 diammonium phosphate Inorganic materials 0.000 claims description 2
- 235000019838 diammonium phosphate Nutrition 0.000 claims description 2
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 claims description 2
- 235000019837 monoammonium phosphate Nutrition 0.000 claims description 2
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 2
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 2
- 229910000403 monosodium phosphate Inorganic materials 0.000 claims description 2
- 235000019799 monosodium phosphate Nutrition 0.000 claims description 2
- 150000003013 phosphoric acid derivatives Chemical class 0.000 claims description 2
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 2
- 229910000160 potassium phosphate Inorganic materials 0.000 claims description 2
- 235000011009 potassium phosphates Nutrition 0.000 claims description 2
- 102000004169 proteins and genes Human genes 0.000 claims description 2
- 108090000623 proteins and genes Proteins 0.000 claims description 2
- 229940057910 shea butter Drugs 0.000 claims description 2
- AJPJDKMHJJGVTQ-UHFFFAOYSA-M sodium dihydrogen phosphate Chemical compound [Na+].OP(O)([O-])=O AJPJDKMHJJGVTQ-UHFFFAOYSA-M 0.000 claims description 2
- 239000001488 sodium phosphate Substances 0.000 claims description 2
- 229910000162 sodium phosphate Inorganic materials 0.000 claims description 2
- 235000011008 sodium phosphates Nutrition 0.000 claims description 2
- 235000020238 sunflower seed Nutrition 0.000 claims description 2
- 239000003760 tallow Substances 0.000 claims description 2
- 235000013337 tricalcium citrate Nutrition 0.000 claims description 2
- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 claims description 2
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- ZOIORXHNWRGPMV-UHFFFAOYSA-N acetic acid;zinc Chemical compound [Zn].CC(O)=O.CC(O)=O ZOIORXHNWRGPMV-UHFFFAOYSA-N 0.000 claims 1
- 235000015278 beef Nutrition 0.000 claims 1
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- XLOMVQKBTHCTTD-UHFFFAOYSA-N Zinc monoxide Chemical compound [Zn]=O XLOMVQKBTHCTTD-UHFFFAOYSA-N 0.000 abstract description 14
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- XYJRXVWERLGGKC-UHFFFAOYSA-D pentacalcium;hydroxide;triphosphate Chemical compound [OH-].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O XYJRXVWERLGGKC-UHFFFAOYSA-D 0.000 description 4
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Images
Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q11/00—Preparations for care of the teeth, of the oral cavity or of dentures; Dentifrices, e.g. toothpastes; Mouth rinses
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/20—Chemical, physico-chemical or functional or structural properties of the composition as a whole
- A61K2800/28—Rubbing or scrubbing compositions; Peeling or abrasive compositions; Containing exfoliants
Abstract
The invention discloses preparation and application of antibacterial enamel remineralization liquid, and particularly relates to the field of oral care. An antibacterial enamel remineralizing fluid comprises a water-in-oil-in-water emulsion containing a mixed salt of soluble calcium, zinc and silver; a water-in-oil-in-water emulsion containing a soluble phosphate; a water-in-oil-in-water emulsion comprising a soluble base. According to the invention, zinc and silver are loaded on the surface of enamel in a loading manner, so that the enamel has antibacterial and anti-inflammatory effects, and the washing-off water-in-oil-in-water emulsion avoids renal metabolism toxicity caused by oral administration of zinc oxide and silver oxide, and is safe and reliable.
Description
Technical Field
The invention belongs to the technical field of daily chemical products, oral hard tissue medical treatment, nursing and cleaning products, particularly relates to medical and oral cleaning and nursing products, and more particularly relates to preparation and application of antibacterial enamel remineralization liquid.
Background
Human enamel is rich in 96% of inorganic hydroxyapatite, researches show that enamel columns in the enamel are in a core-shell structure, organic liposome and other inorganic elements are mainly concentrated in cores of the enamel columns, and the shell structure of the enamel columns is mainly inorganic hydroxyapatite.
Hydroxyapatite (HAP) is a sparingly soluble substance, and therefore the tooth body presents a process of dissolution remineralization in the saliva of the mouth, and a method of achieving enamel remineralization by providing calcium, phosphorus ions has been demonstrated.
The acidic substances generated by food and bacteria can easily demineralize enamel, so that white spots are formed, and finally, the dental caries is evolved; damaged enamel can be repaired to a certain extent by adding calcium and phosphorus ions. However, under the influence of bacteria on the surface of the tooth enamel and the formed bacterial film, the remineralization process of the tooth enamel is limited, the bacteria continuously grow and continuously produce acid, so that the final demineralization rate of the tooth enamel is far higher than the remineralization rate, and the teeth form acid erosion. Therefore, the method effectively inhibits the propagation of bacteria on the surface of the enamel and loads anti-acid-etching substances on the tooth body is an ideal strategy for effectively solving the acid etching and demineralization of the enamel.
The antibacterial and antiviral properties of zinc ions and silver ions and the safety performance of the zinc ions and silver ions in use in human bodies are widely proved, particularly, compounds such as zinc oxide, zinc chloride and zinc lactate rich in zinc element are commonly used in toothpaste, however, bacteria continuously grow in the oral cavity, the tooth brushing time is very limited (usually 3 minutes), partial bacteria can be killed in the process of using the toothpaste by directly adding the silver ions and the zinc ions into the toothpaste, but the bacteria producing acid still continuously exist and damage teeth in a certain period of time after tooth brushing; therefore, zinc oxide and silver ions with antibacterial performance are directly loaded on the enamel, and the continuous inhibition of the breeding of bacteria on the teeth is an ideal strategy for effectively solving the problem of enamel acid etching and demineralization.
Disclosure of Invention
Therefore, the invention provides preparation and application of antibacterial enamel remineralization liquid, and aims to solve the problem that the existing oral care products have poor effects of remineralization of enamel and antibacterial and antiviral effects.
The invention aims to prepare the water-in-oil-in-water emulsion with the internal water phases of free calcium ions, phosphate ions, zinc ions and silver ions respectively, mix the three emulsions according to different proportions, use a toothbrush to break emulsion on the surface of enamel, and deposit zinc and silver elements on the enamel to play a role in the process of remineralization of the enamel.
In order to achieve the above purpose, the invention provides the following technical scheme:
according to a first aspect of the present invention there is provided an antibacterial enamel remineralisation fluid comprising:
a water-in-oil-in-water emulsion simultaneously containing soluble calcium, zinc and silver salts;
a water-in-oil-in-water emulsion containing a soluble phosphate;
a water-in-oil-in-water emulsion comprising a soluble base.
Wherein the soluble calcium, zinc and silver mixed salt is a mixed solution of soluble calcium salt, soluble silver salt and soluble zinc salt.
According to the invention, the calcium, zinc and silver mixed metal ions, phosphate ions and hydroxide ions subjected to compartmentalization treatment are adopted as raw materials, so that direct contact reaction among ions before application is avoided, in the application process, the calcium, zinc and silver mixed metal ions, the phosphate ions and the hydroxide ions are demulsified and released on the surface of enamel, the tooth body is used as a seed crystal to immediately provide calcium and phosphorus ions to form a calcium phosphate supersaturated solution to promote remineralization of the enamel, and meanwhile, the zinc and silver ions react with OH < - > to form the enamel/zinc oxide and enamel/silver oxide mixed material in the remineralization process.
Further, the water-in-oil-in-water emulsion containing soluble calcium, salt and silver mixed salt/soluble phosphate/soluble base is prepared as follows:
mixing an aqueous solution containing soluble calcium, zinc and silver mixed salt with an oil phase containing solid particles according to a volume ratio of (1-3): (1-19) mixing, stirring for 0.5-120 minutes at the temperature of 30-80 ℃ and the rotating speed of 10-24000 r/min to obtain the water-in-oil emulsion containing soluble calcium, zinc and silver salt; then the water-in-oil emulsion containing the soluble calcium, zinc and silver mixed salt and the water phase containing the solid particles are mixed according to the volume ratio (1-3): (1-19) mixing, and stirring for 0.5-120 minutes at the temperature of 30-80 ℃ and the rotating speed of 10-24000 r/min to obtain the water-in-oil-in-water emulsion containing the soluble calcium, zinc and silver mixed salt.
It should be noted that the method for preparing the water-in-oil-in-water emulsion containing soluble phosphate and the water-in-oil-in-water emulsion containing soluble alkali is the same as that of the water-in-oil-in-water emulsion containing soluble calcium, zinc and silver mixed salt, except that the soluble salt is different, and the soluble calcium, zinc and silver mixed salt used in the water-in-oil-water emulsion containing soluble calcium, zinc and silver mixed salt is replaced by the soluble phosphate, or the soluble alkali can be prepared into the water-in-oil-water emulsion containing soluble phosphate and the water-in-oil-water emulsion containing soluble alkali.
Further, the concentration of each of calcium, zinc and silver ions in the aqueous solution containing soluble calcium, zinc and silver mixed salt is 0.001-2.5 mol/l;
and/or the concentration of phosphate ions in the water solution containing soluble phosphate is 0.001-1.5 mol/l;
and/or the concentration of hydroxide ions in the aqueous solution containing the soluble alkali is 0.001-2 mol/l.
Further, the preparation method of the oil phase containing the solid particles is as follows: solid particles and grease according to the mass ratio (0.1-25): 100, mixing at 30-80 ℃;
and/or the preparation method of the aqueous phase containing the solid particles comprises the following steps: the mass ratio of the solid particles to the water is (0.1-25): 100 are mixed to obtain the product.
Further, the soluble calcium salt is a substance which can be dissolved in water and alcohols and can generate free calcium ions. By way of example, the soluble calcium salt includes, but is not limited to, one or more of calcium chloride, calcium hydroxide, calcium oxide, calcium nitrate, calcium gluconate, calcium bicarbonate, calcium lactate, calcium citrate, calcium acetate.
Further, the soluble silver salt is a substance which can be dissolved in water and alcohol substances and can generate free silver ions; by way of example, the soluble silver salts include, but are not limited to, one or more of nitrate, silver fluoride, silver chlorate, silver perchlorate;
further, the soluble zinc salt is a substance which can be dissolved in water and alcohols and can generate free zinc ions; by way of example, the soluble silver salts include, but are not limited to, one or more of nitrate, silver fluoride, silver chlorate, silver perchlorate;
further, the soluble phosphate is a compound capable of producing free phosphate, hydrogen phosphate or dihydrogen phosphate ions. By way of example, the soluble phosphate salts include, but are not limited to, one or more of potassium phosphate, sodium phosphate, ammonium phosphate, potassium monohydrogen phosphate, sodium monohydrogen phosphate, ammonium monohydrogen phosphate, potassium dihydrogen phosphate, sodium dihydrogen phosphate, and ammonium dihydrogen phosphate.
Further, the soluble base is a substance that is soluble in water and capable of generating free hydroxide ions. By way of example, the soluble base includes, but is not limited to, one or more of sodium hydroxide, potassium hydroxide, lithium hydroxide, calcium hydroxide.
Further, the solid particles are organic micro-nano particles and/or inorganic micro-nano particles. By way of example, the solid particles include, but are not limited to, one or more of calcium carbonate, alumina, silica, calcium phosphate, magnesium aluminum silicate, lignocellulose, protein particles.
Furthermore, the oil is a water agent, a gel agent, natural oil from all plants, animals and minerals for preparing toothpaste, mouthwash or enamel repair gel, or synthetic oil processed by petrochemical products and natural extraction raw materials for oral treatment. By way of example, the oil includes, but is not limited to, one or more of triglyceride, peanut oil, rapeseed oil, lard, tallow, shea butter, sunflower seed oil, walnut oil, soybean oil.
According to the second aspect of the present invention, the antibacterial enamel remineralizing solution is prepared by mixing the water-in-oil-in-water emulsion containing soluble calcium, zinc and silver salt, the water-in-oil-in-water emulsion containing soluble phosphate and the water-in-oil-in-water emulsion containing soluble alkali.
According to a third aspect of the present invention there is provided the use of an antibacterial enamel remineralisation liquid as described above in an oral care product which is a toothpaste, mouthwash or gel.
According to a fourth aspect of the present invention, there is provided an oral care product comprising the antibacterial enamel remineralizing liquid as described above.
According to a fifth aspect of the present invention, there is provided a method for loading zinc silver on enamel, wherein daily tooth brushing is performed using an antibacterial enamel remineralizing fluid as described above or an oral care product as described above, or wherein daily tooth brushing is simulated in artificial saliva.
In some preferred embodiments, the zinc-silver element is deposited on the enamel during its remineralization by daily brushing to function. Or the remineralization of the zinc-silver loaded enamel surface is realized by embedding the discrete enamel with epoxy resin, exposing the enamel surface and simulating the daily tooth brushing process in artificial saliva.
It will be appreciated that in order to achieve a high loading of zinc and silver on the enamel, the oral care product preferably comprises an abrasive material, such as silica or calcium carbonate or dibasic calcium phosphate dihydrate.
The invention has the following advantages:
according to the invention, water-in-oil-in-water emulsions with water phases of free calcium, zinc and silver ions, phosphate ions and hydroxide ions are mixed according to different proportions, and the mixture is demulsified on the surface of enamel by using a toothbrush, so that zinc and silver elements can be loaded on the surface of remineralized enamel in the process of remineralization of the enamel, the hardness of teeth is enhanced, and meanwhile, nano zinc oxide and silver with antibacterial, anti-inflammatory and efficacy can be loaded on the enamel of a human body (living body or in vitro); thereby enhancing the antibacterial property of the tooth surface and applying the efficacy of zinc oxide and silver oxide in organisms to human bodies.
According to the invention, zinc and silver are loaded on the surface of enamel in a loading manner, so that the enamel has antibacterial and anti-inflammatory effects, and the washing-off water-in-oil-in-water emulsion avoids renal metabolism toxicity caused by oral administration of zinc oxide and silver oxide, and is safe and reliable.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below. It should be apparent that the drawings in the following description are merely exemplary, and that other embodiments can be derived from the drawings provided by those of ordinary skill in the art without inventive effort.
The structures, ratios, sizes, and the like shown in the present specification are only used for matching with the contents disclosed in the specification, so as to be understood and read by those skilled in the art, and are not used to limit the conditions that the present invention can be implemented, so that the present invention has no technical significance, and any structural modifications, changes in the ratio relationship, or adjustments of the sizes, without affecting the effects and the achievable by the present invention, should still fall within the range that the technical contents disclosed in the present invention can cover.
FIG. 1 is a scanning electron micrograph of a surface mineralization of an enamel module provided in accordance with the present invention; wherein, a is undemineralized enamel; b is demineralized enamel, c is remineralized enamel after the brushing test of the sample 2, and d is remineralized enamel after the brushing test of the sample 1;
FIG. 2 is a graph showing the distribution of zinc element successfully loaded on the enamel surface;
FIG. 3 is a graph showing the distribution of silver element successfully loaded on the enamel surface according to the present invention;
FIG. 4 is a thickness of biofilm grown on an undemineralized enamel surface provided by the present invention;
FIG. 5 is the thickness of the biofilm growing on the remineralized enamel surface after the brush test of sample 2 provided by the present invention;
fig. 6 is the thickness of the biomineral film grown on the surface of remineralized enamel after the brush test of sample 1 provided by the present invention.
Detailed Description
The present invention is described in terms of particular embodiments, other advantages and features of the invention will become apparent to those skilled in the art from the following disclosure, and it is to be understood that the described embodiments are merely exemplary of the invention and that it is not intended to limit the invention to the particular embodiments disclosed. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
Preparing a water-in-oil-in-water emulsion containing a mixed salt of soluble calcium, zinc and silver:
1) adding 20g of calcium carbonate into 300g of triglyceride, and uniformly stirring to prepare an oil phase for later use; 15g of calcium carbonate is added into 180g of water, heated to 40 ℃, and stirred uniformly to prepare a water phase for later use.
2) Uniformly mixing 50ml of calcium chloride aqueous solution with calcium ion concentration of 0.1mol/L, 10ml of silver nitrate aqueous solution with silver ion concentration of 0.1mol/L and 5ml of zinc chloride aqueous solution with zinc ion concentration of 0.1mol/L, adding the mixture into 200ml of oil phase, heating to 60 ℃, and stirring for 1 minute at 5000 revolutions per minute by using a high-speed dispersion machine to obtain water-in-oil emulsion containing soluble calcium, zinc and silver mixed salt;
3) 100ml of the water-in-oil emulsion is added to 200ml of the aqueous phase, heated to 40 ℃ and stirred for 2 minutes at 2000 rpm with a high-speed disperser, so that a water-in-oil-in-water emulsion containing soluble salts of calcium, zinc and silver is obtained.
Preparation of a water-in-oil-in-water emulsion containing soluble phosphate:
1) adding 20g of silicon dioxide into 500g of peanut oil, and uniformly stirring to obtain an oil phase for later use; 20g of calcium carbonate is added into 500g of water, heated to 40 ℃, and stirred uniformly to prepare a water phase for later use.
2) Adding 100ml of dipotassium phosphate aqueous solution with phosphate radical ion concentration of 0.1mol/L into 300ml of oil phase, heating to 70 ℃, and stirring for 10 minutes at 3000 revolutions/minute by using a high-speed dispersion machine to obtain water-in-oil emulsion containing soluble phosphate;
3) adding 100ml of water-in-oil emulsion into 300ml of water phase, heating to 45 ℃, and stirring for 2 minutes at 2000 rpm by using a high-speed dispersion machine to obtain the water-in-oil-in-water emulsion containing soluble phosphate.
Preparation of a water-in-oil-in-water emulsion containing a soluble base:
1) adding 10g of calcium carbonate into 200g of triglyceride, and uniformly stirring to prepare an oil phase for later use; 15g of calcium carbonate is added into 300g of water, heated to 50 ℃, and stirred uniformly to prepare a water phase for later use.
2) Adding 10ml of sodium hydroxide aqueous solution with the concentration of hydroxide ions of 0.15mol/L into 50ml of oil phase, heating to 40 ℃, and stirring for 4 minutes at 3000 revolutions/minute by using a high-speed dispersion machine to obtain water-in-oil emulsion containing soluble alkali;
3) adding 50ml of water-in-oil emulsion into 200ml of water phase, heating to 45 ℃, and stirring for 3 minutes at 1000 revolutions per minute by using a high-speed dispersion machine to obtain the water-in-oil-in-water emulsion containing the soluble alkali.
The preparation method of the antibacterial enamel remineralization liquid provided by the embodiment is as follows:
mixing the water-in-oil-in-water emulsion containing the soluble calcium, zinc and silver mixed salt, the water-in-oil-in-water emulsion containing the soluble phosphate and the water-in-oil-in-water emulsion containing the soluble alkali according to the volume ratio of 6:3: 1.
Example 2
Preparing a water-in-oil-in-water emulsion containing a mixed salt of soluble calcium, zinc and silver:
1) adding 20g of magnesium aluminum silicate into 300g of soybean oil, and uniformly stirring to obtain an oil phase for later use; adding 30g of magnesium aluminum silicate into 500g of water, heating to 60 ℃, and uniformly stirring to obtain a water phase for later use.
2) Uniformly mixing 100ml of calcium nitrate aqueous solution with calcium ion concentration of 0.5mol/L, 20ml of silver nitrate aqueous solution with silver ion concentration of 0.01mol/L and 50ml of zinc chloride aqueous solution with zinc ion concentration of 0.01mol/L, adding the mixture into 300ml of oil phase, heating the mixture to 50 ℃, and stirring the mixture for 3 minutes at 3000 revolutions per minute by using a high-speed dispersion machine to obtain water-in-oil emulsion containing soluble calcium salt;
3) adding 100ml of water-in-oil emulsion into 300ml of water phase, heating to 60 ℃, and stirring for 5 minutes at 1000 revolutions per minute by using a high-speed dispersion machine to obtain the water-in-oil-in-water emulsion containing soluble calcium salt.
Preparation of a water-in-oil-in-water emulsion containing soluble phosphate:
1) adding 10g of silicon dioxide into 200g of peanut oil, and uniformly stirring to obtain an oil phase for later use; adding 20g of calcium carbonate into 300g of water, heating to 55 ℃, and uniformly stirring to obtain a water phase for later use.
2) Adding 100ml of dipotassium hydrogen phosphate aqueous solution with phosphate radical ion concentration of 0.3mol/L into 200ml of oil phase, heating to 50 ℃, and stirring for 2 minutes at 2000 revolutions/minute by using a high-speed dispersion machine to obtain water-in-oil emulsion containing soluble phosphate;
3) adding 100ml of water-in-oil emulsion into 300ml of water phase, heating to 45 ℃, and stirring for 3 minutes at 3000 r/min by using a high-speed dispersion machine to obtain the water-in-oil-in-water emulsion containing soluble phosphate.
Preparation of a water-in-oil-in-water emulsion containing a soluble base:
1) adding 20g of calcium carbonate into 200g of triglyceride, and uniformly stirring to prepare an oil phase for later use; 15g of calcium carbonate is added into 300g of water, heated to 45 ℃, and stirred uniformly to prepare a water phase for later use.
2) Adding 10ml of potassium hydroxide aqueous solution with the concentration of hydroxide ions of 0.01mol/L into 30ml of oil phase, heating to 40 ℃, and stirring for 3 minutes at 3000 revolutions/minute by using a high-speed dispersion machine to obtain water-in-oil emulsion containing soluble alkali;
3) adding 20ml of water-in-oil emulsion into 80ml of water phase, heating to 50 ℃, and stirring for 5 minutes at 1000 revolutions per minute by using a high-speed dispersion machine to obtain the water-in-oil-in-water emulsion containing the soluble alkali.
The preparation method of the antibacterial enamel remineralization liquid provided by the embodiment is as follows:
mixing the water-in-oil-in-water emulsion containing soluble calcium salt, the water-in-oil-in-water emulsion containing soluble phosphate and the water-in-oil-in-water emulsion containing soluble alkali according to the volume ratio of 8:5: 2.
Example 3
Preparing a water-in-oil-in-water emulsion containing a mixed salt of soluble calcium, zinc and silver:
1) adding 30g of calcium carbonate into 300g of triglyceride, and uniformly stirring to prepare an oil phase for later use; 20g of calcium carbonate is added into 500g of water, heated to 65 ℃, and stirred uniformly to prepare a water phase for later use.
2) Uniformly mixing 100ml of calcium nitrate aqueous solution with calcium ion concentration of 0.01mol/L, 10ml of silver nitrate aqueous solution with silver ion concentration of 0.01mol/L and 20ml of zinc nitrate aqueous solution with zinc ion concentration of 0.01mol/L, adding the mixture into 300ml of oil phase, heating the mixture to 50 ℃, and stirring the mixture for 3 minutes at 3000 revolutions per minute by using a high-speed dispersion machine to obtain water-in-oil emulsion containing soluble calcium salt;
3) adding 100ml of water-in-oil emulsion into 400ml of water phase, heating to 40 ℃, and stirring for 3 minutes at 3000 r/min by using a high-speed dispersion machine to obtain the water-in-oil-in-water emulsion containing soluble calcium salt.
Preparation of a water-in-oil-in-water emulsion containing soluble phosphate:
1) adding 25g of silicon dioxide into 300g of peanut oil, and uniformly stirring to obtain an oil phase for later use; 20g of calcium carbonate is added into 500g of water, heated to 55 ℃, and stirred uniformly to prepare a water phase for later use.
2) Adding 100ml of dipotassium hydrogen phosphate aqueous solution with phosphate radical ion concentration of 0.01mol/L into 300ml of oil phase, heating to 45 ℃, and stirring for 3 minutes at 3000 revolutions/minute by using a high-speed dispersion machine to obtain water-in-oil emulsion containing soluble phosphate;
3) adding 100ml of water-in-oil emulsion into 400ml of water phase, heating to 45 ℃, and stirring for 2 minutes at 5000 r/min by using a high-speed dispersion machine to obtain the water-in-oil-in-water emulsion containing soluble phosphate.
Preparation of water-in-oil-in-water emulsion containing soluble base:
1) adding 10g of calcium carbonate into 100g of triglyceride, and uniformly stirring to prepare an oil phase for later use; adding 20g of calcium carbonate into 300g of water, heating to 50 ℃, and uniformly stirring to obtain a water phase for later use.
2) Adding 10ml of sodium hydroxide aqueous solution with the concentration of hydroxide ions of 0.01mol/L into 40ml of oil phase, heating to 40 ℃, and stirring for 3 minutes at 3000 r/min by using a high-speed dispersion machine to obtain water-in-oil emulsion containing soluble alkali;
3) adding 20ml of water-in-oil emulsion into 80ml of water phase, heating to 40 ℃, and stirring for 3 minutes at 4000 revolutions per minute by using a high-speed dispersion machine to obtain the water-in-oil-in-water emulsion containing the soluble alkali.
The preparation method of the antibacterial enamel remineralization liquid provided by the embodiment is as follows:
mixing the water-in-oil-in-water emulsion containing the soluble calcium, zinc and silver mixed salt, the water-in-oil-in-water emulsion containing the soluble phosphate and the water-in-oil-in-water emulsion containing the soluble alkali according to the volume ratio of 13:7: 2.
Test example 1
Sample 1: 10g of the antibacterial enamel remineralization solution of example 1 was added to 120g of Yunnan white drug powder wintergreen whitening toothpaste and mixed well.
Sample 2: yunnan white drug powder and wintergreen whitening toothpaste.
Brushing the enamel modules with the toothpastes of sample 1 and sample 2 respectively, wherein the tooth enamel modules are brushed 3 times a day for 3 minutes each time, and after 15 days, the mineralization on the enamel surface is observed by a scanning electron microscope and is shown in figure 1, wherein a is undemineralized enamel; b is demineralized enamel, c is remineralized enamel after the brushing test of the sample 2, and d is remineralized enamel after the brushing test of the sample 1; therefore, the load of zinc and silver elements strengthens the remineralization degree of enamel; the distribution of elements of zinc and silver successfully loaded on the enamel surface is shown in fig. 2 and fig. 3, respectively, and fig. 2 is a graph showing the distribution of elements of zinc successfully loaded on the enamel surface; FIG. 3 is a graph showing the distribution of silver successfully loaded onto the enamel surface.
As can be seen from figure 1, figure 2 and figure 3, the zinc and silver elements are successfully loaded on the enamel surface.
Test example 2
Experiment for inhibiting dental caries bacteria (streptococcus mutans)
The enamel modules from the experimental group of test example 1 after brushing were placed in the wells of a 24-well tissue culture plate (one module per well, module not touching the walls of the wells), coated with normal human sterile saliva (1.0 mL/well) for 4h at 37 ℃, and rinsed 2 times with 1.0mL PBS. Two generations of streptococcus mutans are inoculated at 37 ℃ till the logarithmic phase of growth (bacterial liquid concentration is 107cfu/mL), and the streptococcus mutans are cultured for 24h to form a biological membrane.
Rinsing the dental model with 1.0mL sterile PBS (soaking time of each rinsing, 10s) for 2-3 times to remove non-adhesive bacteria; non-invasive confocal imaging was performed using a TCSSP2 confocal microscope with a 488nm Ar/Ar-Kr laser scanning head mounted on a vibration-free stage. The objective lens used was 40 x, the image is three times magnified; LIVE/DEAD staining of samples Using the RBacLightTM bacterial viability kit. Staining time was (9 ± 1) min, staining ratio 1: 1, and staining was achieved at the corresponding wavelength [ Syto 9: 515 nm-530 nm; propidium Iodide (PI): optimal fluorescence signal at >600nm ]; at least 3 independent and representative locations on the biofilm covered dental model were selected for these measurements. Within each zone, the thickest spot is measured by determining the upper and lower limits of the biofilm. As shown in fig. 4, 5, 6, wherein fig. 4 is the undemineralized enamel biofilm thickness; FIG. 5 is the remineralization enamel biofilm thickness after the brush test of sample 2; figure 6 is the remineralization enamel biofilm thickness after the brush test of sample 1.
From fig. 4, fig. 5, fig. 6, it is seen that the bacterial membrane thickness of enamel surface capable of remineralization by compartmentalized calcium and phosphorus ions is comparable to that of the original enamel, the bacterial membrane thickness of enamel surface mineralized by zinc-loaded and silver-loaded remineralization liquid is obviously reduced, and the phenomenon of bacterial death occurs, indicating that the enamel surface mineralized by zinc-loaded and silver-loaded has antibacterial performance.
Although the invention has been described in detail above with reference to a general description and specific examples, it will be apparent to one skilled in the art that modifications or improvements may be made thereto based on the invention. Accordingly, such modifications and improvements are intended to be within the scope of the invention as claimed.
Claims (10)
1. An antibacterial enamel remineralizing fluid, comprising:
a water-in-oil-in-water emulsion containing a soluble calcium, zinc and silver mixed salt;
a water-in-oil-in-water emulsion containing a soluble phosphate;
a water-in-oil-in-water emulsion comprising a soluble base.
2. The antibacterial enamel remineralizing fluid according to claim 1, wherein said water-in-oil-in-water emulsion comprising soluble calcium, zinc and silver mixed salt/soluble phosphate/soluble base is prepared by the following steps:
mixing an aqueous solution containing soluble calcium, zinc and silver mixed salt/phosphate/alkali and an oil phase containing solid particles according to a volume ratio of (1-3): (1-19) mixing, stirring for 0.5-120 minutes at the temperature of 30-80 ℃ and the rotating speed of 10-24000 r/min to obtain water-in-oil emulsion containing soluble calcium, zinc and silver mixed salt/phosphate/alkali; then the water-in-oil emulsion containing soluble calcium, zinc and silver mixed salt/phosphate/alkali and the water phase containing solid particles are mixed according to the volume ratio (1-3): (1-19) mixing, and stirring for 0.5-120 minutes at the temperature of 30-80 ℃ and the rotating speed of 10-24000 r/min to obtain the water-in-oil-in-water emulsion containing soluble calcium, zinc and silver mixed salt/phosphate/alkali.
3. The antibacterial enamel remineralizing fluid according to claim 2,
the concentrations of calcium ions, zinc ions and silver ions in the aqueous solution containing the soluble calcium, zinc ions and silver ions are 0.001-2.5 mol/l;
and/or the concentration of phosphate ions in the water solution containing soluble phosphate is 0.001-1.5 mol/l;
and/or the concentration of hydroxide ions in the aqueous solution containing the soluble alkali is 0.001-2 mol/l.
4. The antibacterial enamel remineralization fluid according to claim 3,
the soluble calcium salt is selected from one or more of calcium chloride, calcium hydroxide, calcium oxide, calcium nitrate, calcium gluconate, calcium bicarbonate, calcium lactate, calcium acetate and calcium citrate;
the soluble silver salt is selected from one or more of nitrate, silver fluoride, silver chlorate and silver perchlorate;
the soluble zinc salt is selected from one or more of zinc nitrate, zinc chloride, zinc sulfate and zinc acetate;
and/or the soluble phosphate is selected from one or more of potassium phosphate, sodium phosphate, ammonium phosphate, potassium monohydrogen phosphate, sodium monohydrogen phosphate, ammonium monohydrogen phosphate, potassium dihydrogen phosphate, sodium dihydrogen phosphate and ammonium dihydrogen phosphate;
and/or the soluble alkali is selected from one or more of sodium hydroxide, potassium hydroxide, lithium hydroxide and calcium hydroxide.
5. The antibacterial enamel remineralizing fluid according to claim 2,
the preparation method of the oil phase containing the solid particles comprises the following steps: solid particles and grease according to the mass ratio (0.1-25): 100, mixing at 30-80 ℃;
and/or the preparation method of the aqueous phase containing the solid particles comprises the following steps: solid particles and water according to the mass ratio (0.1-25): 100 are mixed to obtain the product.
6. The antibacterial enamel remineralizing fluid of claim 5,
the solid particles are selected from one or more of calcium carbonate, alumina, silica, calcium phosphate, magnesium aluminum silicate, lignocellulose and protein particles;
and/or the grease is selected from one or more of triglyceride, peanut oil, rapeseed oil, lard, beef tallow, shea butter, sunflower seed oil, walnut oil and soybean oil.
7. The method of any one of claims 1-6, wherein the water-in-oil-in-water emulsion comprising soluble calcium, zinc and silver salts, the water-in-oil-in-water emulsion comprising soluble phosphate salts, and the water-in-oil-in-water emulsion comprising soluble bases are mixed.
8. Use of the antibacterial enamel remineralizing fluid of any one of claims 1-6 in an oral care product which is a toothpaste, mouthwash or gel.
9. An oral care product comprising the antibacterial enamel remineralizing liquid according to any one of claims 1 to 6.
10. A method for loading zinc and silver on enamel, which comprises using an oral care product prepared from the antibacterial enamel remineralizing agent according to any one of claims 1 to 6 or the oral care product according to claim 9 to brush teeth daily, or simulating daily brushing in artificial saliva.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210635367.5A CN115024988B (en) | 2022-06-07 | 2022-06-07 | Preparation and application of antibacterial enamel remineralizing liquid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210635367.5A CN115024988B (en) | 2022-06-07 | 2022-06-07 | Preparation and application of antibacterial enamel remineralizing liquid |
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| Publication Number | Publication Date |
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| CN115024988A true CN115024988A (en) | 2022-09-09 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN116270254A (en) * | 2022-11-28 | 2023-06-23 | 昆明理工大学 | Preparation method and application of amorphous calcium phosphate-cerium phosphate composite material |
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| CN116270254A (en) * | 2022-11-28 | 2023-06-23 | 昆明理工大学 | Preparation method and application of amorphous calcium phosphate-cerium phosphate composite material |
| CN116270254B (en) * | 2022-11-28 | 2024-03-19 | 昆明理工大学 | Preparation method and application of amorphous calcium phosphate-cerium phosphate composite material |
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| CN115024988B (en) | 2023-10-17 |
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