JP2010035552A5 - - Google Patents

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JP2010035552A5
JP2010035552A5 JP2009140192A JP2009140192A JP2010035552A5 JP 2010035552 A5 JP2010035552 A5 JP 2010035552A5 JP 2009140192 A JP2009140192 A JP 2009140192A JP 2009140192 A JP2009140192 A JP 2009140192A JP 2010035552 A5 JP2010035552 A5 JP 2010035552A5
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seq
base sequence
set forth
sequence set
oligonucleotide
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JP2010035552A (en
JP5224539B2 (en
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Claims (12)

下記（ａ）から（ｃ）の工程を含む、サルモネラの血清型がTyphimuriumであるか否かを判別する方法；
（ａ）サルモネラよりゲノムDNAを抽出する工程、
（ｂ）下記（１）〜（４）に記載のDNAを増幅する工程、及び
（ｃ）下記（１）〜（４）に記載のDNAの増幅産物が得られた場合に、サルモネラの血清型がTyphimuriumであると示される工程。
（１）配列番号：１に記載の塩基配列の全部又は一部を含むDNA
（２）配列番号：２に記載の塩基配列の全部又は一部を含むDNA
（３）配列番号：３に記載の塩基配列の全部又は一部を含むDNA
（４）配列番号：４に記載の塩基配列の全部又は一部を含むDNA A method for determining whether or not the Salmonella serotype is Typhimurium, comprising the following steps (a) to (c):
(A) a step of extracting genomic DNA from Salmonella,
(B) a step of amplifying the DNA described in (1) to (4) below; and (c) a serotype of Salmonella when the amplification product of the DNA described in (1) to (4) below is obtained. The process indicated as Typhimurium.
(1) DNA comprising all or part of the base sequence set forth in SEQ ID NO: 1
(2) DNA containing all or part of the base sequence described in SEQ ID NO: 2
(3) DNA comprising all or part of the base sequence set forth in SEQ ID NO: 3
(4) DNA comprising all or part of the base sequence set forth in SEQ ID NO: 4 下記（ｄ）及び（ｅ）の工程をさらに含む、請求項２に記載の方法；
（ｄ）サルモネラのＯ群血清型を判定する工程、及び
（ｅ）工程（ｄ）においてＯ群血清型がＯ４群と判定された場合に、サルモネラの血清型がTyphimuriumであると示される工程。 The method according to claim 2, further comprising the following steps (d) and (e):
(D) a step of determining Salmonella group O serotype, and (e) a step in which the Salmonella serotype is Typhimurium when the group O serotype is determined to be the O4 group in step (d). 配列番号：１〜４記載の塩基配列の全部又は一部を含むDNAとストリンジェントな条件で特異的にハイブリダイズするオリゴヌクレオチド。 An oligonucleotide that specifically hybridizes under stringent conditions with DNA comprising all or part of the base sequences described in SEQ ID NOs: 1-4 . 配列番号：２５〜３２のいずれかに記載の塩基配列を含むオリゴヌクレオチド。 An oligonucleotide comprising the base sequence described in any one of SEQ ID NOs: 25 to 32 . 下記（１）から（４）を含むセット
（１）配列番号：２５に記載の塩基配列を含むオリゴヌクレオチド、及び、配列番号：２６に記載の塩基配列を含むオリゴヌクレオチドのセット、
（２）配列番号：２７に記載の塩基配列を含むオリゴヌクレオチド、及び、配列番号：２８に記載の塩基配列を含むオリゴヌクレオチドのセット、
（３）配列番号：２９に記載の塩基配列を含むオリゴヌクレオチド、及び、配列番号：３０に記載の塩基配列を含むオリゴヌクレオチドのセット、
（４）配列番号：３１に記載の塩基配列を含むオリゴヌクレオチド、及び、配列番号：３２に記載の塩基配列を含むオリゴヌクレオチドのセット。 A set comprising (1) to (4) below (1) an oligonucleotide comprising the nucleotide sequence set forth in SEQ ID NO: 25, and a set of oligonucleotides comprising the nucleotide sequence set forth in SEQ ID NO: 26;
(2) a set of oligonucleotides containing the base sequence set forth in SEQ ID NO: 27 and oligonucleotides containing the base sequence set forth in SEQ ID NO: 28;
(3) an oligonucleotide comprising the base sequence set forth in SEQ ID NO: 29, and a set of oligonucleotides comprising the base sequence set forth in SEQ ID NO: 30;
(4) A set of oligonucleotides containing the base sequence set forth in SEQ ID NO: 31 and oligonucleotides containing the base sequence set forth in SEQ ID NO: 32. 請求項３又は４に記載のオリゴヌクレオチド、又は請求項５に記載のセットを含有するサルモネラの血清型判別用キット。 A salmonella serotyping kit comprising the oligonucleotide according to claim 3 or 4 or the set according to claim 5 . Salmonella TyphimuriumのサルモネラのゲノムDNAを鋳型とし、下記（ｉ）のセットをプライマーセットとするPCRによって増幅されるPCR産物；
（ｉ）下記（１）から（４）を含むセット
（１）配列番号：２５に記載の塩基配列を含むオリゴヌクレオチド、及び、配列番号：２６に記載の塩基配列を含むオリゴヌクレオチドのセット、
（２）配列番号：２７に記載の塩基配列を含むオリゴヌクレオチド、及び、配列番号：２８に記載の塩基配列を含むオリゴヌクレオチドのセット、
（３）配列番号：２９に記載の塩基配列を含むオリゴヌクレオチド、及び、配列番号：３０に記載の塩基配列を含むオリゴヌクレオチドのセット、
（４）配列番号：３１に記載の塩基配列を含むオリゴヌクレオチド、及び、配列番号：３２に記載の塩基配列を含むオリゴヌクレオチドのセット。 PCR product amplified by PCR using Salmonella Typhimurium Salmonella genomic DNA as a template and the following (i) set as a primer set;
(I) a set comprising the following (1) to (4) (1) an oligonucleotide comprising the base sequence set forth in SEQ ID NO: 25, and a set of oligonucleotides comprising the base sequence set forth in SEQ ID NO: 26,
(2) a set of oligonucleotides containing the base sequence set forth in SEQ ID NO: 27 and oligonucleotides containing the base sequence set forth in SEQ ID NO: 28;
(3) an oligonucleotide comprising the base sequence set forth in SEQ ID NO: 29, and a set of oligonucleotides comprising the base sequence set forth in SEQ ID NO: 30;
(4) A set of oligonucleotides containing the base sequence set forth in SEQ ID NO: 31 and oligonucleotides containing the base sequence set forth in SEQ ID NO: 32. 配列番号：９９に記載の塩基配列の全部又は一部を含むDNAとストリンジェントな条件で特異的にハイブリダイズするオリゴヌクレオチド。 An oligonucleotide that specifically hybridizes under stringent conditions with DNA comprising all or part of the base sequence set forth in SEQ ID NO: 99 . 配列番号：７３および７４のいずれかに記載の塩基配列を含むオリゴヌクレオチド。 An oligonucleotide comprising the base sequence described in any one of SEQ ID NOs: 73 and 74 . 配列番号：７３に記載の塩基配列を含むオリゴヌクレオチド、及び、配列番号：７４に記載の塩基配列を含むオリゴヌクレオチドのセット。 A set of an oligonucleotide containing the base sequence set forth in SEQ ID NO: 73 and an oligonucleotide containing the base sequence set forth in SEQ ID NO: 74. 請求項９に記載のオリゴヌクレオチド、又は請求項１０に記載のセットを含有するサルモネラの血清型判別用キット。 A salmonella serotyping kit comprising the oligonucleotide according to claim 9 or the set according to claim 10 . Salmonella TyphimuriumのサルモネラのゲノムDNAを鋳型とし、下記（１）のセットをプライマーセットとするPCRによって増幅されるPCR産物；
（１）配列番号：７３に記載の塩基配列を含むオリゴヌクレオチド、及び、配列番号：７４に記載の塩基配列を含むオリゴヌクレオチドのセット。 PCR products amplified by PCR using Salmonella Typhimurium Salmonella genomic DNA as a template and the following (1) set as a primer set;
(1) A set of an oligonucleotide containing the base sequence set forth in SEQ ID NO: 73 and an oligonucleotide containing the base sequence set forth in SEQ ID NO: 74.
JP2009140192A 2008-07-08 2009-06-11 Rapid identification of Salmonella Typhimurium by multiplex detection of specific genes Active JP5224539B2 (en)

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JP2011170210A Division JP5224557B2 (en) 2008-07-08 2011-08-03 Rapid identification of Salmonella choleraesis by multiplex detection of specific genes
JP2011170233A Division JP5279050B2 (en) 2008-07-08 2011-08-03 Rapid identification method of Salmonella Dublin by multiplex detection of specific genes
JP2011170228A Division JP5279049B2 (en) 2008-07-08 2011-08-03 Rapid identification of Salmonella Enteritidis by multiplex detection of specific genes
JP2011170239A Division JP5224558B2 (en) 2008-07-08 2011-08-03 Rapid identification of Salmonella Gallinarum by multiplex detection of specific genes

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JP2011170233A Active JP5279050B2 (en) 2008-07-08 2011-08-03 Rapid identification method of Salmonella Dublin by multiplex detection of specific genes
JP2011170228A Active JP5279049B2 (en) 2008-07-08 2011-08-03 Rapid identification of Salmonella Enteritidis by multiplex detection of specific genes
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JP2011170228A Active JP5279049B2 (en) 2008-07-08 2011-08-03 Rapid identification of Salmonella Enteritidis by multiplex detection of specific genes
JP2011170210A Active JP5224557B2 (en) 2008-07-08 2011-08-03 Rapid identification of Salmonella choleraesis by multiplex detection of specific genes

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JP5727255B2 (en) * 2011-02-21 2015-06-03 日本ハム株式会社 Rapid and simple discrimination method of Salmonella serotype by multiplex PCR and its primer set
JP5279051B2 (en) * 2011-08-10 2013-09-04 独立行政法人農業・食品産業技術総合研究機構 Oligonucleotides for detecting Salmonella Typhimurium and rapid serotype identification method using them
CA2920636A1 (en) * 2013-08-20 2015-02-23 E. I. Du Pont De Nemours And Company Sequences and their use for detection of salmonella enteritidis and/or salmonella typhimurium
CN106226536B (en) * 2016-08-01 2017-11-10 福建省产品质量检验研究院 A kind of position phase polymorphism test method of salmonella H antigens
CN106244690B (en) * 2016-08-03 2019-06-18 扬州大学 A kind of multiple PCR detection kit of Rapid identification Salmonella enteritidis, white diarrhea/Salmonella gallinarum and Salmonella dublin
CN108148892B (en) * 2018-01-24 2021-07-27 深圳市儿童医院 PCR (polymerase chain reaction) detection primer, nucleic acid hybridization membrane strip, kit and method for salmonella
CN111733266A (en) * 2020-07-13 2020-10-02 扬州大学 PCR detection kit for rapidly detecting salmonella and identifying pullorum/typhoid salmonella and application thereof
CN113388539B (en) * 2020-12-30 2022-05-20 广东省微生物研究所（广东省微生物分析检测中心） Salmonella standard strain containing specific molecular target and detection and application thereof

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