JP2013055956A5 - - Google Patents

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JP2013055956A5
JP2013055956A5 JP2012271425A JP2012271425A JP2013055956A5 JP 2013055956 A5 JP2013055956 A5 JP 2013055956A5 JP 2012271425 A JP2012271425 A JP 2012271425A JP 2012271425 A JP2012271425 A JP 2012271425A JP 2013055956 A5 JP2013055956 A5 JP 2013055956A5
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単離ＤＮＡ分子であって、配列番号２１として同定されたヌクレオチド配列を含み、かつイベントＴＣ１５０７ゲノムに含まれる配列からなる、単離ＤＮＡ分子。 An isolated DNA molecule comprising the nucleotide sequence identified as SEQ ID NO: 21 and consisting of the sequence contained in the event TC1507 genome. 単離ＤＮＡ分子であって、配列番号２２として同定されたヌクレオチド配列を含み、かつイベントＴＣ１５０７ゲノムに含まれる配列からなる、単離ＤＮＡ分子。 An isolated DNA molecule comprising the nucleotide sequence identified as SEQ ID NO: 22 and consisting of the sequence contained in the event TC1507 genome. 単離ＤＮＡ分子であって、配列番号２４として同定されたヌクレオチド配列を含み、かつイベントＴＣ１５０７ゲノムに含まれる配列からなる、単離ＤＮＡ分子。 An isolated DNA molecule comprising the nucleotide sequence identified as SEQ ID NO: 24 and consisting of the sequence contained in the event TC1507 genome. 単離ＤＮＡ分子であって、配列番号２６として同定されたヌクレオチド配列を含み、かつイベントＴＣ１５０７ゲノムに含まれる配列からなる、単離ＤＮＡ分子。 An isolated DNA molecule comprising the nucleotide sequence identified as SEQ ID NO: 26 and consisting of the sequence contained in the event TC1507 genome. 単離ＤＮＡ分子であって、配列番号２７として同定されたヌクレオチド配列を含み、かつイベントＴＣ１５０７ゲノムに含まれる配列からなる、単離ＤＮＡ分子。 An isolated DNA molecule comprising the nucleotide sequence identified as SEQ ID NO: 27 and consisting of the sequence contained in the event TC1507 genome. ＴＣ１５０７特異的領域を検出する生物学的サンプル内のイベントＴＣ１５０７を同定するためのキットであって、該キットは、第１のプライマーおよび第２のプライマーを備え、該第１のプライマーが配列番号２６内の配列または配列番号２７内の配列を認識し、該第２のプライマーが配列番号２５内の配列または配列番号２２内の配列を認識し、該配列が、配列番号１、２、３、４、５、６、７、８、９、１０、１１、１２、１３、１４、１５、１６、１７、１８、１９、２０、および、２３、ならびに、その相補体からなる群から選択される、キット。 A kit for identifying event TC1507 in a biological sample that detects a TC1507 specific region, the kit comprising a first primer and a second primer, wherein the first primer is SEQ ID NO: 26. Or the sequence within SEQ ID NO: 27, the second primer recognizes the sequence within SEQ ID NO: 25 or the sequence within SEQ ID NO: 22, and the sequence is identified as SEQ ID NO: 1, 2, 3, 4 Selected from the group consisting of 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, and 23, and their complements; kit. 前記少なくとも第１および第２のプライマーがそれぞれ配列番号１の配列および配列番号２の配列を含む、請求項６に記載のキット。 The kit of claim 6, wherein the at least first and second primers comprise the sequence of SEQ ID NO: 1 and the sequence of SEQ ID NO: 2, respectively. 前記少なくとも第１および第２のプライマーがそれぞれ配列番号２の配列および配列番号２３の配列を含む、請求項６に記載のキット。 The kit of claim 6, wherein the at least first and second primers comprise the sequence of SEQ ID NO: 2 and the sequence of SEQ ID NO: 23, respectively. 前記少なくとも第１および第２のプライマーがそれぞれ配列番号３の配列および配列番号５の配列を含む、請求項６に記載のキット。 The kit of claim 6, wherein the at least first and second primers comprise the sequence of SEQ ID NO: 3 and the sequence of SEQ ID NO: 5, respectively. 前記少なくとも第１および第２のプライマーがそれぞれ配列番号４の配列および配列番号５の配列を含む、請求項６に記載のキット。 The kit of claim 6, wherein the at least first and second primers comprise the sequence of SEQ ID NO: 4 and the sequence of SEQ ID NO: 5, respectively. トウモロコシのイベントＴＣ１５０７およびその子孫を検出するためのキットであって、該キットは、以下：
（ａ）配列番号４５、４６、４７、４９、５０、５１、５２、５４、５５、５６および５７ならびにその相補体からなる群より選択されるヌクレオチド配列；または
（ｂ）配列番号４５、４６、４７、４９、５０、５１、５２、５４、５５、５６および５７ならびにその相補体からなる群より選択されるヌクレオチド配列のフラグメントであって、該フラグメントは、少なくとも１８ヌクレオチド長である、フラグメント、
を含む少なくとも１つのＤＮＡ分子を備える、キット。 A kit for detecting corn event TC1507 and its progeny, the kit comprising:
(A) a nucleotide sequence selected from the group consisting of SEQ ID NOs: 45, 46, 47, 49, 50, 51, 52, 54, 55, 56 and 57 and their complements; or (b) SEQ ID NOs: 45, 46, A fragment of a nucleotide sequence selected from the group consisting of 47, 49, 50, 51, 52, 54, 55, 56 and 57 and its complement, wherein the fragment is at least 18 nucleotides in length,
A kit comprising at least one DNA molecule comprising: 生物学的サンプル内のイベントＴＣ１５０７を同定するためのキットであって、該キットは、少なくとも１つの特異的プローブを備え、ここで、該プローブは、以下：
（ａ）配列番号４５、４６、４７、４９、５０、５１、５２、５４、５５、５６および５７ならびにその相補体からなる群より選択されるヌクレオチド配列；または
（ｂ）配列番号４５、４６、４７、４９、５０、５１、５２、５４、５５、５６および５７ならびにその相補体からなる群より選択されるヌクレオチド配列のフラグメントであって、該プローブは、少なくとも１８ヌクレオチド長である、フラグメント、
を含む、キット。 A kit for identifying event TC1507 in a biological sample, the kit comprising at least one singular probe, wherein said probe comprises:
(A) a nucleotide sequence selected from the group consisting of SEQ ID NOs: 45, 46, 47, 49, 50, 51, 52, 54, 55, 56 and 57 and their complements; or
(B) a fragment of a nucleotide sequence selected from the group consisting of SEQ ID NOs: 45, 46, 47, 49, 50, 51, 52, 54, 55, 56 and 57 and their complements, the probe comprising at least A fragment that is 18 nucleotides long,
Including a kit. 生物学的サンプル中のイベントＴＣ１５０７を同定するための方法であって、該方法は、以下：
（ａ）配列番号４５、４６、４７、４９、５０、５１、５２、５４、５５、５６および５７ならびにその相補体からなる群より選択される配列；または
（ｂ）配列番号４５、４６、４７、４９、５０、５１、５２、５４、５５、５６および５７ならびにその相補体からなる群より選択されるヌクレオチド配列のフラグメントであって、該フラグメントは、少なくとも１８ヌクレオチド長である、フラグメント、
を含むプローブを用いて、配列番号４５、４６、４７、４９、５０、５１、５２、５４、５５、５６および５７からなる群より選択される配列を検出する工程を包含する、方法。 A method for identifying event TC1507 in a biological sample, the method comprising:
(A) a sequence selected from the group consisting of SEQ ID NOs: 45, 46, 47, 49, 50, 51, 52, 54, 55, 56 and 57 and their complements; or (b) SEQ ID NOs: 45, 46, 47 49, 50, 51, 52, 54, 55, 56 and 57 and a fragment of a nucleotide sequence selected from the group consisting of the complement thereof, the fragment being at least 18 nucleotides in length,
Detecting a sequence selected from the group consisting of SEQ ID NOs: 45, 46, 47, 49, 50, 51, 52, 54, 55, 56 and 57, using a probe comprising: 生物学的サンプル中のイベントＴＣ１５０７を同定するための方法であって、該方法は、２種類のプライマーを用いたポリメラーゼ鎖反応を使用して前記生物学的サンプル中に存在する核酸からＤＮＡフラグメントを増幅する工程を包含し、該プライマー配列が、配列番号１、２、３、４、５、６、７、８、９、１０、１１、１２、１３、１４、１５、１６、１７、１８、１９、２０、および、２３、ならびに、その相補体からなる群から選択される、請求項１６に記載の方法。 A method for identifying event TC1507 in a biological sample, comprising: using a polymerase chain reaction with two primers to remove a DNA fragment from nucleic acid present in said biological sample. the step of amplifying and packaging containing, the primer sequence, SEQ ID NO: 1,2,3,4,5,6,7,8,9,10,11,12,13,14,15,16,17,18 19. The method of claim 16, wherein the method is selected from the group consisting of: 19, 20, and 23, and their complements . 前記第１のプライマーおよび前記第２のプライマーがそれぞれ、配列番号２の配列および配列番号１の配列を含む、請求項１４に記載の方法。 The first primer and the second primer each comprise a sequence and sequence of SEQ ID NO: 1 SEQ ID NO: 2, The method of claim 1 4. 前記第１のプライマーおよび前記第２のプライマーがそれぞれ、配列番号２の配列および配列番号２３の配列を含む、請求項１４に記載の方法。 The first primer and the second primer each comprise a sequence of SEQ and SEQ ID NO: 23 SEQ ID NO: 2, The method of claim 1 4. 前記第１のプライマーおよび前記第２のプライマーがそれぞれ、配列番号３の配列および配列番号５の配列を含む、請求項１４に記載の方法。 The first primer and the second primer each comprise a sequence and sequence of SEQ ID NO: 5 SEQ ID NO: 3 The method of claim 1 4. 前記第１のプライマーおよび前記第２のプライマーがそれぞれ、配列番号４の配列および配列番号５の配列を含む、請求項１４に記載の方法。 The first primer and the second primer each comprise a sequence and sequence of SEQ ID NO: 5 SEQ ID NO: 4, The method of claim 1 4. 生物学的サンプル中のトウモロコシのイベントＴＣ１５０７またはその子孫の存在を検出するための方法であって、該方法は、以下：
（ａ）該生物学的サンプルからＤＮＡサンプルを抽出する工程；
（ｂ）ＤＮＡプライマー分子である配列番号１および配列番号２を提供する工程；
（ｃ）ＤＮＡ増幅反応条件を提供する工程；
（ｄ）該ＤＮＡ増幅反応を実行して、それによってＤＮＡ単位複製配列分子を生成する工程；および
（ｅ）該ＤＮＡ単位複製配列分子を検出する工程であって、ここで、該ＤＮＡ増幅反応での該ＤＮＡ単位複製配列分子の検出がトウモロコシのイベントＴＣ１５０７の存在を示す、工程、
を包含する、方法。 A method for detecting the presence of corn event TC1507 or its progeny in a biological sample, the method comprising:
(A) extracting a DNA sample from the biological sample;
(B) providing DNA primer molecules SEQ ID NO: 1 and SEQ ID NO: 2;
(C) providing DNA amplification reaction conditions;
(D) performing the DNA amplification reaction, thereby generating a DNA amplicon molecule; and (e) detecting the DNA amplicon molecule, wherein the DNA amplification reaction comprises Detection of said DNA amplicon molecule indicates the presence of corn event TC1507,
Including the method. 生物学的サンプル中のトウモロコシのイベントＴＣ１５０７またはその子孫の存在を検出するための方法であって、該方法は、以下：
（ａ）該生物学的サンプルからＤＮＡサンプルを抽出する工程；
（ｂ）ＤＮＡプライマー分子である配列番号２および配列番号２３を提供する工程；
（ｃ）ＤＮＡ増幅反応条件を提供する工程；
（ｄ）該ＤＮＡ増幅反応を実行して、それによってＤＮＡ単位複製配列分子を生成する工程；および
（ｅ）該ＤＮＡ単位複製配列分子を検出する工程であって、ここで、該ＤＮＡ増幅反応での該ＤＮＡ単位複製配列分子の検出がトウモロコシのイベントＴＣ１５０７の存在を示す、工程、
を包含する、方法。 A method for detecting the presence of corn event TC1507 or its progeny in a biological sample, the method comprising:
(A) extracting a DNA sample from the biological sample;
(B) providing DNA primer molecules SEQ ID NO: 2 and SEQ ID NO: 23;
(C) providing DNA amplification reaction conditions;
(D) performing the DNA amplification reaction, thereby generating a DNA amplicon molecule; and (e) detecting the DNA amplicon molecule, wherein the DNA amplification reaction comprises Detection of said DNA amplicon molecule indicates the presence of corn event TC1507,
Including the method. 生物学的サンプル中のトウモロコシのイベントＴＣ１５０７またはその子孫の存在を検出するための方法であって、該方法は、以下：
（ａ）該生物学的サンプルからＤＮＡサンプルを抽出する工程；
（ｂ）ＤＮＡプライマー分子である配列番号３および配列番号５を提供する工程；
（ｃ）ＤＮＡ増幅反応条件を提供する工程；
（ｄ）該ＤＮＡ増幅反応を実行して、それによってＤＮＡ単位複製配列分子を生成する工程；および
（ｅ）該ＤＮＡ単位複製配列分子を検出する工程であって、ここで、該ＤＮＡ増幅反応での該ＤＮＡ単位複製配列分子の検出がトウモロコシのイベントＴＣ１５０７の存在を示す、工程、
を包含する、方法。 A method for detecting the presence of corn event TC1507 or its progeny in a biological sample, the method comprising:
(A) extracting a DNA sample from the biological sample;
(B) providing DNA primer molecules SEQ ID NO: 3 and SEQ ID NO: 5;
(C) providing DNA amplification reaction conditions;
(D) performing the DNA amplification reaction, thereby generating a DNA amplicon molecule; and (e) detecting the DNA amplicon molecule, wherein the DNA amplification reaction comprises Detection of said DNA amplicon molecule indicates the presence of corn event TC1507,
Including the method. 生物学的サンプル中のトウモロコシのイベントＴＣ１５０７またはその子孫の存在を検出するための方法であって、該方法は、以下：
（ａ）該生物学的サンプルからＤＮＡサンプルを抽出する工程；
（ｂ）ＤＮＡプライマー分子である配列番号４および配列番号５を提供する工程；
（ｃ）ＤＮＡ増幅反応条件を提供する工程；
（ｄ）該ＤＮＡ増幅反応を実行して、それによってＤＮＡ単位複製配列分子を生成する工程；および
（ｅ）該ＤＮＡ単位複製配列分子を検出する工程であって、ここで、該ＤＮＡ増幅反応での該ＤＮＡ単位複製配列分子の検出がトウモロコシのイベントＴＣ１５０７の存在を示す、工程、
を包含する、方法。 A method for detecting the presence of corn event TC1507 or its progeny in a biological sample, the method comprising:
(A) extracting a DNA sample from the biological sample;
(B) providing DNA primer molecules SEQ ID NO: 4 and SEQ ID NO: 5;
(C) providing DNA amplification reaction conditions;
(D) performing the DNA amplification reaction, thereby generating a DNA amplicon molecule; and (e) detecting the DNA amplicon molecule, wherein the DNA amplification reaction comprises Detection of said DNA amplicon molecule indicates the presence of corn event TC1507,
Including the method. （ａ）生物学的サンプルからＤＮＡサンプルを抽出する工程；
（ｂ）ＤＮＡプライマー分子である配列番号１および配列番号２を提供する工程；
（ｃ）ＤＮＡ増幅反応条件を提供する工程；ならびに
（ｄ）該ＤＮＡ増幅反応を実行して、それによってＤＮＡ単位複製配列分子を生成する工程
によって生産された単位複製配列を含み、かつイベントＴＣ１５０７ゲノムに含まれる配列からなる、単離ＤＮＡ分子。 (A) extracting a DNA sample from a biological sample;
(B) providing DNA primer molecules SEQ ID NO: 1 and SEQ ID NO: 2;
(C) providing a DNA amplification reaction condition; and (d) comprising the amplicon produced by the step of performing the DNA amplification reaction, thereby generating a DNA amplicon molecule, and the event TC1507 genome An isolated DNA molecule consisting of the sequence contained in （ａ）生物学的サンプルからＤＮＡサンプルを抽出する工程；
（ｂ）ＤＮＡプライマー分子である配列番号２および配列番号２３を提供する工程；
（ｃ）ＤＮＡ増幅反応条件を提供する工程；ならびに
（ｄ）該ＤＮＡ増幅反応を実行して、それによってＤＮＡ単位複製配列分子を生成する工程
によって生産された単位複製配列を含み、かつイベントＴＣ１５０７ゲノムに含まれる配列からなる、単離ＤＮＡ分子。 (A) extracting a DNA sample from a biological sample;
(B) providing DNA primer molecules SEQ ID NO: 2 and SEQ ID NO: 23;
(C) providing a DNA amplification reaction condition; and (d) comprising the amplicon produced by the step of performing the DNA amplification reaction, thereby generating a DNA amplicon molecule, and the event TC1507 genome An isolated DNA molecule consisting of the sequence contained in （ａ）生物学的サンプルからＤＮＡサンプルを抽出する工程；
（ｂ）ＤＮＡプライマー分子である配列番号３および配列番号５を提供する工程；
（ｃ）ＤＮＡ増幅反応条件を提供する工程；ならびに
（ｄ）該ＤＮＡ増幅反応を実行して、それによってＤＮＡ単位複製配列分子を生成する工程
によって生産された単位複製配列を含み、かつイベントＴＣ１５０７ゲノムに含まれる配列からなる、単離ＤＮＡ分子。 (A) extracting a DNA sample from a biological sample;
(B) providing DNA primer molecules SEQ ID NO: 3 and SEQ ID NO: 5;
(C) providing a DNA amplification reaction condition; and (d) comprising the amplicon produced by the step of performing the DNA amplification reaction, thereby generating a DNA amplicon molecule, and the event TC1507 genome An isolated DNA molecule consisting of the sequence contained in （ａ）生物学的サンプルからＤＮＡサンプルを抽出する工程；
（ｂ）ＤＮＡプライマー分子である配列番号４および配列番号５を提供する工程；
（ｃ）ＤＮＡ増幅反応条件を提供する工程；ならびに
（ｄ）該ＤＮＡ増幅反応を実行して、それによってＤＮＡ単位複製配列分子を生成する工程
によって生産された単位複製配列を含み、かつイベントＴＣ１５０７ゲノムに含まれる配列からなる、単離ＤＮＡ分子。 (A) extracting a DNA sample from a biological sample;
(B) providing DNA primer molecules SEQ ID NO: 4 and SEQ ID NO: 5;
(C) providing a DNA amplification reaction condition; and (d) comprising the amplicon produced by the step of performing the DNA amplification reaction, thereby generating a DNA amplicon molecule, and the event TC1507 genome An isolated DNA molecule consisting of the sequence contained in サンプル中のＴＣ１５０７イベントに対応するＤＮＡの存在を検出するための方法であって、該方法は、以下：
ａ．トウモロコシＤＮＡを含むサンプルと、ポリヌクレオチドプローブとを接触させる工程であって、該プローブが、以下：
（ｉ）配列番号４５、４６、４７、４９、５０、５１、５２、５４、５５、５６および５７ならびにその相補体からなる群より選択される少なくとも１つのＤＮＡ配列；または
（ｉｉ）配列番号４５、４６、４７、４９、５０、５１、５２、５４、５５、５６および５７ならびにその相補体からなる群より選択されるヌクレオチド配列のフラグメントであって、該フラグメントは、少なくとも１８ヌクレオチド長である、フラグメント、
を含む、工程；
ｂ．該サンプルおよびプローブを高ストリンジェントなハイブリダイゼーション条件に供する工程；ならびに
ｃ．該ＤＮＡに対する該プローブのハイブリダイゼーションを検出する工程であって、ここで、ハイブリダイゼーションの検出が該ＴＣ１５０７イベントの存在を示す、工程、を包含する、方法。 A method for detecting the presence of DNA corresponding to a TC1507 event in a sample, the method comprising:
a. Contacting a sample comprising corn DNA with a polynucleotide probe, the probe comprising:
(I) at least one DNA sequence selected from the group consisting of SEQ ID NOs: 45, 46, 47, 49, 50, 51, 52, 54, 55, 56 and 57 and their complements; or
(Ii) a fragment of a nucleotide sequence selected from the group consisting of SEQ ID NOs: 45, 46, 47, 49, 50, 51, 52, 54, 55, 56 and 57 and complements thereof, the fragment comprising at least A fragment that is 18 nucleotides long,
Comprising a step ;
b. Subjecting the sample and probe to highly stringent hybridization conditions; and c. Detecting the hybridization of the probe to the DNA, wherein detecting hybridization indicates the presence of the TC1507 event. 配列番号１、２、３、４、２３、４５、４６、４７、４９、５０、５１、５２、５４、５５、５６および５７からなる群より選択される配列またはその相補体を含み、かつイベントＴＣ１５０７ゲノムに含まれる配列からなる、単離核酸。 A sequence selected from the group consisting of SEQ ID NOs: 1, 2, 3, 4, 23, 45, 46, 47, 49, 50, 51, 52, 54, 55, 56 and 57, or a complement thereof, and an event An isolated nucleic acid consisting of a sequence contained in the TC1507 genome. 配列番号１、２、３、４、および２３からなる群より選択される配列またはその相補体を含み、かつイベントＴＣ１５０７ゲノムに含まれる配列からなる、請求項２８に記載の単離核酸。 29. The isolated nucleic acid of claim 28 , comprising a sequence selected from the group consisting of SEQ ID NOs: 1, 2, 3, 4, and 23, or a complement thereof, and consisting of a sequence contained in the event TC1507 genome. 一対のＤＮＡ分子であって、該一対のＤＮＡ分子は、以下：第１のＤＮＡ分子と第２のＤＮＡ分子とを含み、ここで、該ＤＮＡ分子が、ＴＣ１５０７トウモロコシ植物またはその子孫から抽出されたＤＮＡにとって特徴的であるＤＮＡプライマーまたはプローブとして機能する、少なくとも１８隣接ヌクレオチド長の、配列番号２６のヌクレオチドまたはその相補体のフラグメントである、一対のＤＮＡ分子。 A pair of DNA molecules, the pair of DNA molecules comprising: a first DNA molecule and a second DNA molecule, wherein the DNA molecule was extracted from a TC1507 corn plant or its progeny A pair of DNA molecules that are at least 18 contiguous nucleotides in length, the nucleotide of SEQ ID NO: 26, or a fragment of its complement that functions as a DNA primer or probe that is characteristic of DNA. 一対のＤＮＡ分子であって、該一対のＤＮＡ分子は、以下：第１のＤＮＡ分子と第２のＤＮＡ分子とを含み、ここで、該ＤＮＡ分子が、ＴＣ１５０７トウモロコシ植物またはその子孫から抽出されたＤＮＡにとって特徴的であるＤＮＡプライマーまたはプローブとして機能する、少なくとも１８隣接ヌクレオチド長の、配列番号２７のヌクレオチドまたはその相補体のフラグメントである、一対のＤＮＡ分子。 A pair of DNA molecules, the pair of DNA molecules comprising: a first DNA molecule and a second DNA molecule, wherein the DNA molecule was extracted from a TC1507 corn plant or its progeny A pair of DNA molecules that are at least 18 contiguous nucleotides in length, the nucleotide of SEQ ID NO: 27, or a fragment of its complement that functions as a DNA primer or probe that is characteristic of DNA. 配列番号４５、４６、４７、４９、５０、５１、５２、５４、５５、５６および５７ならびにその相補体からなる群より選択される配列を含み、かつイベントＴＣ１５０７ゲノムに含まれる配列からなる、単離ＤＮＡ分子。 Comprising a sequence selected from the group consisting of SEQ ID NOs: 45, 46, 47, 49, 50, 51, 52, 54, 55, 56 and 57 and their complements, and consisting of a sequence contained in the Event TC1507 genome. Isolated DNA molecule. 種子サンプル中で、配列番号２６または配列番号２７内の配列を特異的に認識する特異的プライマーまたはプローブを用いて、ＴＣ１５０７特異的領域を検出する工程を包含し、そして、該プライマー配列またはプローブ配列が、配列番号１、２、３、４、５、２３、４５、４６、４７、４９、５０、５１、５２、５４、５５、５６、および、５７、ならびに、その相補体からなる群から選択される、種子純度を確認するための方法。 Detecting a TC1507 specific region using a specific primer or probe that specifically recognizes a sequence within SEQ ID NO: 26 or SEQ ID NO: 27 in a seed sample, and said primer sequence or probe sequence Is selected from the group consisting of SEQ ID NOs: 1, 2, 3, 4, 5, 23, 45, 46, 47, 49, 50, 51, 52, 54, 55, 56, and 57, and their complements It is the method for confirming seed purity. イベントＴＣ１５０７の存在について種子をスクリーニングする方法であって、１つの種子ロットのサンプル中で、配列番号２６または配列番号２７内の配列を特異的に認識する特異的プライマーまたはプローブを用いて、ＴＣ１５０７特異的領域を検出する工程を包含し、そして、該プライマー配列またはプローブ配列が、配列番号１、２、３、４、５、２３、４５、４６、４７、４９、５０、５１、５２、５４、５５、５６、および、５７、ならびに、その相補体からなる群から選択される、方法。 A method of screening seeds for the presence of event TC1507, using a specific primer or probe that specifically recognizes a sequence within SEQ ID NO: 26 or SEQ ID NO: 27 in a sample of one seed lot, Detecting the target region, and the primer sequence or probe sequence is SEQ ID NO: 1, 2, 3, 4, 5, 23, 45, 46, 47, 49, 50, 51, 52, 54, 55. A method selected from the group consisting of 55, 56 and 57, and their complements . 昆虫抵抗性トウモロコシ植物、またはその一部分であって、ここで、配列番号２４のヌクレオチド配列を有するＤＮＡが、該植物のゲノムの一部分を形成する、昆虫抵抗性トウモロコシ植物、またはその一部分。 An insect resistant corn plant, or part thereof, wherein the DNA having the nucleotide sequence of SEQ ID NO: 24 forms part of the genome of the plant, or part thereof. 請求項３５に記載の昆虫抵抗性トウモロコシ植物の後代植物であって、ここで、配列番号２４のヌクレオチド配列を有するＤＮＡが、該植物のゲノムの一部分を形成する、後代植物。 36. A progeny plant of the insect-resistant corn plant according to claim 35 , wherein the DNA having the nucleotide sequence of SEQ ID NO: 24 forms part of the plant's genome. 配列番号２４のヌクレオチド配列を有するＤＮＡが種子のゲノムの一部分を形成する、請求項３５または３６に記載の植物の種子。 37. The plant seed of claim 35 or 36 , wherein the DNA having the nucleotide sequence of SEQ ID NO: 24 forms part of the seed genome. 一対の単離核酸であって、ＤＮＡ増幅手順で一緒に使用される場合、イベントＴＣ１５０７にとって特徴的な単位複製配列を生産し、ここで、該対の各メンバーは該単位複製配列の反対の端に存在し、該対の各メンバーは、配列番号１、２、３、４、５、６、７、８、９、１０、１１、１２、１３、１４、１５、１６、１７、１８、１９、２０、および、２３、ならびに、その相補体からなる群から選択される、一対の単離核酸。 A pair of isolated nucleic acids, when used together in a DNA amplification procedure, produces a characteristic amplicon for event TC1507, where each member of the pair is the opposite end of the amplicon. exist, each member of the pair, SEQ ID NO: 1,2,3,4,5,6,7,8,9,10,11,12,13,14,15,16,17,18,19 A pair of isolated nucleic acids selected from the group consisting of, 20, and 23 and their complements . トウモロコシ組織へのＴＣ１５０７イベント挿入の存在を検出するための方法であって、該方法は、以下：
（ａ）配列番号２６または配列番号２７由来の少なくとも１０個のヌクレオチドを、各々、含むプライマー対を選択する工程；
（ｂ）該トウモロコシ組織のサンプルと該プライマー対とを接触させる工程；および
（ｃ）ＤＮＡ増幅を実行し、そして単位複製配列について分析する工程、
を包含し、ここで、該プライマー対が、配列番号１、２、３、４、５、６、７、８、９、１０、１１、１２、１３、１４、１５、１６、１７、１８、１９、２０、２３、４５、４６、４７、４９、５０、５１、５２、５４、５５、５６および５７ならびにそれらの相補体からなる群より選択される、方法。 A method for detecting the presence of a TC1507 event insertion in corn tissue, the method comprising:
(A) selecting a primer pair each comprising at least 10 nucleotides from SEQ ID NO: 26 or SEQ ID NO: 27;
(B) contacting the sample of corn tissue with the primer pair; and (c) performing DNA amplification and analyzing for amplicons;
Wherein the primer pair is SEQ ID NO: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, A method selected from the group consisting of 19, 20, 23, 45, 46, 47, 49, 50, 51, 52, 54, 55, 56 and 57 and their complements. トウモロコシ組織へのイベントＴＣ１５０７挿入の存在を検出するための方法であって、該方法は、以下：
（ａ）該トウモロコシ組織のサンプルと、ポリヌクレオチドプローブとを接触させる工程であって、該プローブが、以下：
（ｉ）配列番号４５、４６、４７、４９、５０、５１、５２、５４、５５、５６および５７ならびにその相補体からなる群より選択されるヌクレオチド配列；または
（ｉｉ）配列番号４５、４６、４７、４９、５０、５１、５２、５４、５５、５６および５７ならびにその相補体からなる群より選択されるヌクレオチド配列のフラグメントであって、該プローブは、少なくとも１８ヌクレオチド長である、フラグメント、
を含む、工程；
（ｂ）該サンプルおよびプローブを、高ストリンジェントなハイブリダイゼーション条件に供する工程；ならびに
（ｃ）該プローブのハイブリダイゼーションについて分析する工程、
包含する、方法。 A method for detecting the presence of an event TC1507 insertion in corn tissue, the method comprising:
(A) a sample of the corn tissue, comprising contacting the polynucleotide probe, the probe is selected from the group consisting of:
(I) a nucleotide sequence selected from the group consisting of SEQ ID NOs: 45, 46, 47, 49, 50, 51, 52, 54, 55, 56 and 57 and their complements; or
(Ii) a fragment of a nucleotide sequence selected from the group consisting of SEQ ID NOs: 45, 46, 47, 49, 50, 51, 52, 54, 55, 56 and 57 and their complements, the probe comprising at least A fragment that is 18 nucleotides long,
Comprising a step;
(B) subjecting the sample and probe to highly stringent hybridization conditions; and (c) analyzing for hybridization of the probe;
The method of inclusion. ポリヌクレオチドプローブを備える、キットであって、該プローブが、以下：
（ｉ）配列番号４５、４６、４７、４９、５０、５１、５２、５４、５５、５６および５７ならびにその相補体からなる群より選択されるヌクレオチド配列；または
（ｉｉ）配列番号４５、４６、４７、４９、５０、５１、５２、５４、５５、５６および５７ならびにその相補体からなる群より選択されるヌクレオチド配列のフラグメントであって、該フラグメントは、少なくとも１８ヌクレオチド長である、フラグメント、
を含む、キット。 A kit comprising a polynucleotide probe, the probe comprising:
(I) a nucleotide sequence selected from the group consisting of SEQ ID NOs: 45, 46, 47, 49, 50, 51, 52, 54, 55, 56 and 57 and their complements; or
(Ii) a fragment of a nucleotide sequence selected from the group consisting of SEQ ID NOs: 45, 46, 47, 49, 50, 51, 52, 54, 55, 56 and 57 and complements thereof, the fragment comprising at least A fragment that is 18 nucleotides long,
Including a kit . プライマー対を備えるキットであって、該プライマーの各々は、配列番号２６および配列番号２７由来の少なくとも１０個のヌクレオチドを各々含み、ここで、該プライマー対が、配列番号１、２、３、４、５、６、７、８、９、１０、１１、１２、１３、１４、１５、１６、１７、１８、１９、２０、２３、４５、４６、４７、４９、５０、５１、５２、５４、５５、５６および５７ならびにそれらの相補体からなる群より選択される、キット。 A kit comprising a primer pair, each of the primers comprising at least 10 nucleotides from SEQ ID NO: 26 and SEQ ID NO: 27, respectively, wherein the primer pair comprises SEQ ID NO: 1, 2, 3, 4 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 23, 45, 46, 47, 49, 50, 51, 52, 54 , 55, 56 and 57 and their complements.
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