CN106226536B - A kind of position phase polymorphism test method of salmonella H antigens - Google Patents
A kind of position phase polymorphism test method of salmonella H antigens Download PDFInfo
- Publication number
- CN106226536B CN106226536B CN201610618448.9A CN201610618448A CN106226536B CN 106226536 B CN106226536 B CN 106226536B CN 201610618448 A CN201610618448 A CN 201610618448A CN 106226536 B CN106226536 B CN 106226536B
- Authority
- CN
- China
- Prior art keywords
- phase
- antigens
- salmonella
- culture
- sterile
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/86—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood coagulating time or factors, or their receptors
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56911—Bacteria
- G01N33/56916—Enterobacteria, e.g. shigella, salmonella, klebsiella, serratia
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
- G01N2333/24—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
- G01N2333/255—Salmonella (G)
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Hematology (AREA)
- Immunology (AREA)
- Urology & Nephrology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Food Science & Technology (AREA)
- Physics & Mathematics (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention discloses a kind of position phase polymorphism test method of salmonella H antigens, it carries out the agglutination reaction of serum of O antigens to Salmonella strains to be checked, 1st phase and the 2nd phase H factors are examined according to a point public sentiment condition for O antigens, when it is not single-phase bacterium only to detect a phase and bacterial strain to be checked, it is defined as aimed strain, known phase H factor serums and aimed strain are added in sterile vegetative meat soup afterwards, bacterial sediment is collected by centrifugation after culture, the slide agglutination inspection that bacteria suspension is used to treat phase-detecting H antigens is obtained after washing bacterium.The slide agglutination speed of H antigens is fast after our tagmeme phase polymorphism, phenomenon is obvious, interference is small, reproducible, success rate is high, easy to operate, has higher feasibility and practicality.
Description
Technical field
The present invention relates to a kind of position phase polymorphism test method of salmonella H antigens.
Background technology
Salmonella is zoonosis pathogen significant on public hygienics, its correct serotype
It is significant in infectious disease control.2500 kinds of Salmonella serogroup has been detected at present, existing more than 290 serotype in China
Report.The Serotype Identification of salmonella is more complicated, and its serotype is together decided on by O antigens, the 1st phase and the 2nd phase H antigens etc.
（Some only have a phase H factors）, and the measure of H antigens is the Main Basiss of salmonella serotype.H antigens are located at flagellum
On, it is in the nature unstable proteantigen, there is the factor such as stronger specificity, heating, dry, freezing or Ethanol Treatment
Flagella structural is damaged, causes H antigenicities to weaken, so as to bring difficulty to appraisal.For example, there is experimenter to find in freezer
Resting period it is longer sample separation salmonella strain, reach H antigenicity restore needed for passage number it is more.Daily identification work
The flagellum depauperation of salmonella is frequently encountered in work and the indefinite situation of parting, in standard GB/T 4789.4-2010
In recommend simple flat board method, glass-tube method and the traditional position phase polymorphism method of 3 kinds of voltage regulator tube method to induce another phase H factors.Other
Scientific worker also discloses that the modification method of some phase polymorphisms experiment, such as nutrient broth-nutrient agar method, coubling dilution
Deng.Wherein simple flat board method is widely used because its is simple and convenient, but its distinct disadvantage is that success rate is low；Glass-tube method and voltage regulator tube method
Material there are certain requirements, such as capillary and small voltage regulator tube, and complex operation.In addition the above method uses nutrient agar or semisolid
Substrate of the soft agar as strain growth, the presence of agar suppress the mobility of culture medium, and the kinetic characteristic of this and flagellum is present
Antagonism, so as to hinder the antigenic recoveries of H.In addition, salmonella can break through low concentration agar, diffuse to inside culture medium
Growth, cause thalline picking difficult, therefore when carrying out the slide agglutination test of H antigens, culture medium is inevitably with thalline
Into in serum drop, and can not be completely dispersed, small solid medium particle is easily obscured with serum agglutination particle, so as to
The judgement of severe jamming aggegation result.In addition, the agglutinator of H antigens to be cotton-shaped, is not easy to observe, generally required in experiment picking compared with
For more thalline to strengthen aggegation effect, this is conflicting with the purpose for reducing solid medium interference.In view of the foregoing, at present
Position phase polymorphism test method exist interference is big, success rate is low, complex operation, it is time-consuming more the shortcomings of, can not meet increasing
Inspection work demand.
The content of the invention
For disadvantages mentioned above, it is an object of the invention to provide a kind of success rate is high, reproducible, interference is low, operation letter
Just the efficiently position phase polymorphism test method of salmonella H antigens, to reach above-mentioned purpose, the technical solution adopted by the present invention
It is：
A kind of position phase polymorphism test method of salmonella H antigens, comprises the following steps：
A. Salmonella strains to be checked are carried out with the agglutination reaction of serum of O antigens, divides public sentiment condition successively according to O antigens
The 1st phase and the 2nd phase H factors are checked, when it is not single-phase bacterium only to detect a phase and bacterial strain to be checked, is defined as aimed strain；
B. known phase H factor serums are taken into sterile vegetative meat soup（The ratio between volume of serum and nutrient broth is 1:200~
1:800）, mix, vaccination target bacterial strain, in 36 DEG C of ± 1 DEG C of culture h of 18 h ~ 24, obtain culture；
C. culture is transferred in sterile centrifugation tube, 5 ~ 10 min is centrifuged under the conditions of 5000 ~ 8000 r/min, shifting is abandoned
Supernatant, retain the bacterial sediment of centrifugation bottom of the tube；
D. sterile saline is added into the centrifuge tube containing bacterial sediment（The volume of sterile saline and culture
The ratio between be 1:1~1:3）, with pipettor gently pressure-vaccum, until thalline suspends completely, again under the conditions of 5000 ~ 8000 r/min
5 ~ 10 min are centrifuged, supernatant is abandoned in shifting, and bacterium step is washed in completion；Washing bacterium step can be repeated 1 times or repeatedly；
E. washed to completion in the centrifuge tube of bacterium step and add sterile saline（The volume of bacterial sediment and sterile physiological salt
The ratio between be 1:2~1:5）, with pipettor, gently pressure-vaccum mixes, and obtains bacteria suspension；
F. 10 ~ 15 μ L are taken to treat that phase-detecting H factor serums are added dropwise on clean slide, taking 1 ~ 2 μ L bacteria suspensions to be added to serum
In drop, mix and tilt shake slide, agglutination phenomenon is observed within 1 min；Simultaneously in another region sterile physiological of slide
Salt solution replaces treating phase-detecting H factor serums, is used as and compares after being mixed with bacteria suspension.
Compared with prior art, the beneficial effects of the invention are as follows：Culture containing agar is replaced using the nutrient broth of liquid
Substrate of the base as strain growth, eliminate the antagonism that interference and flagellum of the agar to slide agglutination are grown.Liquid
The composition of culture medium is uniform, and microorganism can fully contact and utilize the nutriment in culture medium, motion of the flagellum as salmonella
Organ, motion effect can be fully played in liquid medium within and is significantly induced to grow, more conducively H is antigenic extensive
It is multiple.During agglutination test, higher H antigenicities become apparent from agglutination phenomenon, and without the interference of solid medium particle, aggegation knot
Fruit is easier to observe and judged.
The present invention is by being collected by centrifugation bacterial sediment, it is easier to obtains more thalline to realize the mesh of enhancing aggegation effect
, thalline relative distribution used is suspended in bacteria suspension during agglutination test, it is easier to is spread in serum uniform, makes aggegation
Speed faster, phenomenon become apparent from, disturb it is smaller.In addition, the experimental condition and each component that specify that each step quantitative in method
Dosage, make that the repeatability of phase polymorphism experiment is more preferable, has higher success rate.
Embodiment
In order that the objects, technical solutions and advantages of the present invention become apparent from, with reference to embodiments, the present invention is carried out
It is further to describe in detail, it will be appreciated that described embodiment is only explaining the present invention.Every skill based on the present invention
The simplification or equivalent variations that art scheme is made, belong to protection scope of the present invention.
Embodiment 1:
The blind sample examination of 2015 food security sampling observation detection Cheng Jian mechanisms of state food pharmaceuticals administration general bureau（Salmonella
Examine）, totally 10 parts of samples, numbering are CODE1 ~ 10, if detection salmonella need to carry out serotype to isolated strains.Through inspection
Survey, totally 4 parts of samples are that salmonella is positive by CODE1, CODE4, CODE5, CODE6 in 10 parts of samples.Enter according to the following steps afterwards
Row follow-up test：
A. 4 Salmonella strains to be checked are carried out with the agglutination reaction of serum of O antigens, divides public sentiment condition according to O antigens
It is examined in the 1st phase and the 2nd phase H factors, qualification result CODE1（O 7:H r）、CODE4（O 4:H i）、CODE5（O 4:H
f,g,s）、CODE6（O 19:H i）, wherein CODE5 bacterial strain to be checked is single-phase bacterium, so determining CODE1, CODE4, CODE6
The salmonella positive strain of totally 3 samples is aimed strain.
B. known phase H factor serums are taken into sterile vegetative meat soup（The ratio between volume of serum and nutrient broth is 1:200）,
Mix, vaccination target bacterial strain, in 36 DEG C of ± 1 DEG C of 18 h of culture, obtain culture；
C. culture is transferred in sterile centrifugation tube, 10 min is centrifuged under the conditions of 5000 r/min, supernatant is abandoned in shifting, is protected
Stay the bacterial sediment of centrifugation bottom of the tube；
D. sterile saline is added into the centrifuge tube containing bacterial sediment（The volume of sterile saline and culture
The ratio between be 1:1）, with pipettor gently pressure-vaccum, until thalline suspends completely, centrifuge 10 under the conditions of 5000 r/min again
Supernatant is abandoned in min, shifting, and bacterium step is washed in completion；
E. washed to completion in the centrifuge tube of bacterium step and add sterile saline（The volume of bacterial sediment and sterile physiological salt
The ratio between be 1:2）, with pipettor, gently pressure-vaccum mixes, and obtains bacteria suspension；
F. 10 μ L are taken to treat that phase-detecting H factor serums are added dropwise on clean slide, taking 1 μ L bacteria suspensions to be added to serum drop
In, mix and tilt shake slide, agglutination phenomenon is observed within 1 min；Simultaneously in another region sterile saline of slide
Instead for the treatment of phase-detecting H factor serums, it is used as and compares after being mixed with bacteria suspension；H antigens position phase polymorphism result of the test is CODE1（H 7）、
CODE4（H 2）、CODE6（H z6）.
The complete qualification result of serotype is as shown in table 1, while carries out multiple put down to qualification result with simple flat board method
Row checking, confirmatory experiment show that the qualification result of two methods is identical, and completely the same with blind sample examination measurement result.
Embodiment 2:
This grade of blind sample examination of food security sampling observation detection Cheng Jian mechanisms of office of state food pharmaceuticals administration general bureau in 2016
Totally 10 parts of samples, numbering are CODE1 ~ 10 for work, wherein salmonella inspection, and report detects or do not detected salmonella.Through inspection
Surveying CODE2, CODE7, CODE8 in 10 parts of samples, totally 3 samples are that salmonella is positive.Subsequently tried according to the following steps afterwards
Test：
A. 3 Salmonella strains to be checked are carried out with the agglutination reaction of serum of O antigens, divides public sentiment condition according to O antigens
It is examined in the 1st phase and the 2nd phase H factors, inspection result CODE2（O 7:H c）；CODE7（O 4:H f,g,s）、CODE8（O
7:H c）, wherein CODE7 bacterial strain to be checked is single-phase bacterium, so determining that the salmonella of CODE2, CODE8 totally 2 samples is positive
Bacterial strain is aimed strain.
B. known phase H factor serums are taken into sterile vegetative meat soup（The ratio between volume of serum and nutrient broth is 1:500）,
Mix, vaccination target bacterial strain, in 36 DEG C of ± 1 DEG C of 20 h of culture, obtain culture；
C. culture is transferred in sterile centrifugation tube, 5 min is centrifuged under the conditions of 6000 r/min, supernatant is abandoned in shifting, is protected
Stay the bacterial sediment of centrifugation bottom of the tube；
D. sterile saline is added into the centrifuge tube containing bacterial sediment（The volume of sterile saline and culture
The ratio between be 1:2）, with pipettor gently pressure-vaccum, until thalline suspends completely, 5 min are centrifuged under the conditions of 6000 r/min again,
Supernatant is abandoned in shifting, and bacterium step is washed in completion；
E. washed to completion in the centrifuge tube of bacterium step and add sterile saline（The volume of bacterial sediment and sterile physiological salt
The ratio between be 1:3）, with pipettor, gently pressure-vaccum mixes, and obtains bacteria suspension；
F. 13 μ L are taken to treat that phase-detecting H factor serums are added dropwise on clean slide, taking 1 μ L bacteria suspensions to be added to serum drop
In, mix and tilt shake slide, agglutination phenomenon is observed within 1 min；Simultaneously in another region sterile saline of slide
Instead for the treatment of phase-detecting H factor serums, it is used as and compares after being mixed with bacteria suspension；H antigens position phase polymorphism result of the test is CODE2（H 5）、
CODE8（H 5）.
The complete qualification result of serotype is as shown in table 2, equally serotype result is carried out with simple flat board method parallel
Checking, the result show that the qualification result of two methods is completely the same.
Embodiment 3:
To one plant of salmonella typhimurium（Strain number：CGMCC1.1174）Carry out the confirmatory experiment of serotype.Will
Follow-up test is carried out according to the following steps after bacterial strain activation：
A. Salmonella strains to be checked are carried out with the agglutination reaction of serum of O antigens, divides public sentiment condition successively according to O antigens
The 1st phase and the 2nd phase H factors are checked, qualification result is O 4:H i, so being defined as aimed strain;
B. known phase H factor serums are taken into sterile vegetative meat soup（The ratio between volume of serum and nutrient broth is 1:800）,
Mix, vaccination target bacterial strain, in 36 DEG C of ± 1 DEG C of 24 h of culture, obtain culture；
C. culture is transferred in sterile centrifugation tube, 5 min is centrifuged under the conditions of 8000 r/min, supernatant is abandoned in shifting, is protected
Stay the bacterial sediment of centrifugation bottom of the tube；
D. sterile saline is added into the centrifuge tube containing bacterial sediment（The volume of sterile saline and culture
The ratio between be 1:3）, with pipettor gently pressure-vaccum, until thalline suspends completely, 5 min are centrifuged under the conditions of 8000 r/min again,
Supernatant is abandoned in shifting, and bacterium step is washed in completion；Bacterium step is washed to repeat 1 time；
E. washed to completion in the centrifuge tube of bacterium step and add sterile saline（The volume of bacterial sediment and sterile physiological salt
The ratio between be 1:5）, with pipettor, gently pressure-vaccum mixes, and obtains bacteria suspension；
F. 15 μ L are taken to treat that phase-detecting H factor serums are added dropwise on clean slide, taking 2 μ L bacteria suspensions to be added to serum drop
In, mix and tilt shake slide, agglutination phenomenon is observed within 1 min；Simultaneously in another region sterile saline of slide
Instead for the treatment of phase-detecting H factor serums, it is used as and compares after being mixed with bacteria suspension；
The slide agglutination inspection result of 2nd phase H antigens of aimed strain is H 2, meets the serum of salmonella typhimurium
Type feature.
In the implementation process of above-mentioned 3 embodiments, find H when using technical scheme after the phase polymorphism of position because
Sub- serum agglutination speed faster, phenomenon become apparent from, disturb small, the repeatability of position phase polymorphism experiment is more preferable, has higher success rate.Reliably
Qualification result also illustrate that the position phase polymorphism test method of salmonella H antigens of the present invention has higher feasibility and practicality
Property.
Those of ordinary skill in the art are familiar with it should be appreciated that the experimental condition of technical solution of the present invention is included necessarily
Scope, such as：The ratio between volume of serum and nutrient broth is 1 during training objective bacterial strain:200~1:800, the culture of aimed strain
Time is 18 ~ 24 h, when culture is centrifuged centrifugal rotational speed be 5000 ~ 8000 r/min, centrifugation time be 5 ~ 10 min, wash bacterium
The ratio between sterile saline and the volume of culture are 1 in step:1~1:3, washing bacterium step can be repeated 1 times or repeatedly, make
The ratio between volume of bacterial sediment and sterile physiological salt is 1 during standby bacteria suspension:2~1:5, it is added dropwise on clean slide and treats the phase-detecting H factors
The dripping quantity of serum is 10 ~ 15 μ L during serum, and the addition of bacteria suspension is 1 ~ 2 μ L when bacteria suspension is added into serum drop.
When using the experimental condition in above range, technical scheme can also reach embodiment 1, embodiment 2 or embodiment 3
Identical effect.
Above by description of listed embodiment, the basic ideas and basic principles of the present invention are elaborated.Obviously, institute
The embodiment stated is only the part of the embodiment of the present invention, and non-limiting exhaustion.Based on the embodiment in the present invention, ability
All other embodiment that domain those of ordinary skill is obtained on the premise of creative work is not paid, belongs to the present invention
Protection domain.
Claims (1)
1. a kind of position phase polymorphism test method of the salmonella H antigens of non-disease diagnosis and therapeutic purposes, comprises the following steps：
A. Salmonella strains to be checked are carried out with the agglutination reaction of serum of O antigens, is examined according to a point public sentiment condition for O antigens
1st phase and the 2nd phase H factors, when it is not single-phase bacterium only to detect a phase and bacterial strain to be checked, it is defined as aimed strain；
B. taking known phase H factor serums, the ratio between volume of serum and nutrient broth is 1 into sterile vegetative meat soup:200~1:
800, mix, vaccination target bacterial strain, in 36 DEG C of ± 1 DEG C of culture h of 18 h ~ 24, obtain culture；
C. culture is transferred in sterile centrifugation tube, 5 ~ 10 min is centrifuged under the conditions of 5000 ~ 8000 r/min, shifting is abandoned
Clearly, the bacterial sediment of centrifugation bottom of the tube is retained；
D. sterile saline is added into the centrifuge tube containing bacterial sediment, the ratio between volume of sterile saline and culture
For 1:1~1:3, with pipettor gently pressure-vaccum, until thalline suspends completely, again under the conditions of 5000 ~ 8000 r/min centrifugation 5 ~
Supernatant is abandoned in 10 min, shifting, and bacterium step is washed in completion；Bacterium step is washed to be repeated 1 times or repeatedly；
E. washed to completion in the centrifuge tube of bacterium step and add sterile saline, the ratio between volume of bacterial sediment and sterile physiological salt
For 1:2~1:5, with pipettor, gently pressure-vaccum mixes, and obtains bacteria suspension；
F. 10 ~ 15 μ L are taken to treat that phase-detecting H factor serums are added dropwise on clean slide, taking 1 ~ 2 μ L bacteria suspensions to be added to serum drop
In, mix and tilt shake slide, agglutination phenomenon is observed within 1 min；Simultaneously in another region sterile saline of slide
Instead for the treatment of phase-detecting H factor serums, it is used as and compares after being mixed with bacteria suspension.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610618448.9A CN106226536B (en) | 2016-08-01 | 2016-08-01 | A kind of position phase polymorphism test method of salmonella H antigens |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610618448.9A CN106226536B (en) | 2016-08-01 | 2016-08-01 | A kind of position phase polymorphism test method of salmonella H antigens |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106226536A CN106226536A (en) | 2016-12-14 |
| CN106226536B true CN106226536B (en) | 2017-11-10 |
Family
ID=57536323
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610618448.9A Active CN106226536B (en) | 2016-08-01 | 2016-08-01 | A kind of position phase polymorphism test method of salmonella H antigens |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106226536B (en) |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2063769C1 (en) * | 1992-11-11 | 1996-07-20 | Государственный научно-исследовательский институт биосинтеза белковых веществ | Method of preparing specific salmonellosis polyvalent h-serum for accelerated salmonella detection in foodstuffs, fodder, environment object and clinical material |
| CN1570637A (en) * | 2003-07-25 | 2005-01-26 | 大连康基食品安全检测技术有限公司 | Fast detection device for salmonella |
| CN101955993A (en) * | 2010-06-13 | 2011-01-26 | 中华人民共和国珠海出入境检验检疫局 | Compound gene chip for detecting salmonella serotype and detection method thereof |
| CN102031282A (en) * | 2010-11-19 | 2011-04-27 | 上海市疾病预防控制中心 | Kit of ready-to-use salmonella identification plate, preparation method and use method |
| CN103614453A (en) * | 2013-05-16 | 2014-03-05 | 黑龙江中医药大学 | Gastrointestinal bacteria detection method for medical microbiology experiment |
| CN103725780A (en) * | 2013-12-18 | 2014-04-16 | 广东省微生物研究所 | Method for detecting and identifying salmonella 1, 4, [5], 12:i- |
| CN104059977A (en) * | 2014-06-25 | 2014-09-24 | 上海交通大学 | Salmonella serotype identification method and kit thereof |
| CN104087654A (en) * | 2013-04-01 | 2014-10-08 | 中国农业大学 | Multiple PCR identification kit of salmonella and five serotypes of salmonella |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB9207069D0 (en) * | 1992-03-31 | 1992-05-13 | Mini Agriculture & Fisheries | Method of testing for salmonella |
| AUPQ275799A0 (en) * | 1999-09-10 | 1999-10-07 | Luminis Pty Limited | Recombinant bacterium expressing an oligosaccharide receptor mimic |
| JP5224539B2 (en) * | 2008-07-08 | 2013-07-03 | 独立行政法人農業・食品産業技術総合研究機構 | Rapid identification of Salmonella Typhimurium by multiplex detection of specific genes |
| WO2012058303A2 (en) * | 2010-10-26 | 2012-05-03 | The Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services, Centers For Disease Control And Prevention | Rapid salmonella serotyping assay |
-
2016
- 2016-08-01 CN CN201610618448.9A patent/CN106226536B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2063769C1 (en) * | 1992-11-11 | 1996-07-20 | Государственный научно-исследовательский институт биосинтеза белковых веществ | Method of preparing specific salmonellosis polyvalent h-serum for accelerated salmonella detection in foodstuffs, fodder, environment object and clinical material |
| CN1570637A (en) * | 2003-07-25 | 2005-01-26 | 大连康基食品安全检测技术有限公司 | Fast detection device for salmonella |
| CN101955993A (en) * | 2010-06-13 | 2011-01-26 | 中华人民共和国珠海出入境检验检疫局 | Compound gene chip for detecting salmonella serotype and detection method thereof |
| CN102031282A (en) * | 2010-11-19 | 2011-04-27 | 上海市疾病预防控制中心 | Kit of ready-to-use salmonella identification plate, preparation method and use method |
| CN104087654A (en) * | 2013-04-01 | 2014-10-08 | 中国农业大学 | Multiple PCR identification kit of salmonella and five serotypes of salmonella |
| CN103614453A (en) * | 2013-05-16 | 2014-03-05 | 黑龙江中医药大学 | Gastrointestinal bacteria detection method for medical microbiology experiment |
| CN103725780A (en) * | 2013-12-18 | 2014-04-16 | 广东省微生物研究所 | Method for detecting and identifying salmonella 1, 4, [5], 12:i- |
| CN104059977A (en) * | 2014-06-25 | 2014-09-24 | 上海交通大学 | Salmonella serotype identification method and kit thereof |
Non-Patent Citations (4)
| Title |
|---|
| 1种沙门菌位相变异实验的方法学研究;侯水平 等;《中国卫生检验杂志》;20150630;第25卷(第12期);1935-1936,1940 * |
| A Rapid and Sensitive Method to Identify and Differentiate Salmonella enterica Serotype Typhimurium and Salmonella enterica Serotype 4,[5],12:i:- by Combining Traditional Serotyping and Multiplex Polymerase Chain Reaction;Lisa Barco,et al;《FOODBORNE PATHOGENS AND DISEASE》;20111231;第8卷(第6期);741-743 * |
| M肉汤法在沙门菌血清分型中的应用;邱亚群 等;《热带医学杂志》;20150131;第15卷(第1期);53-55 * |
| Vi-Specific Latex Agglutination for Early and Rapid Detection of Salmonella Serotype typhi in Blood Cultures;Mary V. Jesudason,et al;《DIAGN MICROBIOL INFECT DIS》;19941231;第18卷;75-78 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106226536A (en) | 2016-12-14 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Tan et al. | Safety assessment and probiotic evaluation of Enterococcus faecium YF5 isolated from sourdough | |
| He et al. | Rapid quantification of live/dead lactic acid bacteria in probiotic products using high-sensitivity flow cytometry | |
| Li et al. | Rapid separation and immunoassay for low levels of Salmonella in foods using magnetosome–antibody complex and real‐time fluorescence quantitative PCR | |
| CN109880772B (en) | Method for separating and culturing helicobacter pylori strain | |
| Van Vuurde et al. | Comparison of immunofluorescence colony-staining in media, selective isolation on pectate medium, ELISA and immunofluorescence cell staining for detection of Erwinia carotovora subsp. atroseptica and E. chrysanthemi in cattle manure slurry | |
| CN101892300A (en) | Klebsiella pneumoniae detection kit and use method thereof | |
| Zhang et al. | Rapid detection of Enterobacter cloacae by immunomagnetic separation and a colloidal gold-based immunochromatographic assay | |
| Wen et al. | Rapid on-site detection of viable Escherichia coli O157: H7 in lettuce using immunomagnetic separation combined with PMAxx-LAMP and nucleic acid lateral flow strip | |
| CN107164497A (en) | Loop-mediated isothermal amplification technique detects the primer and kit of pseudomonas aeruginosa | |
| CN112961805B (en) | Salmonella typhimurium with quinolone drug resistance genes gyrA and parE mutated simultaneously and application thereof | |
| Ling et al. | Escherichia coli outer membrane vesicles induced DNA double-strand breaks in intestinal epithelial Caco-2 cells | |
| CN112961804B (en) | Salmonella typhimurium and application thereof | |
| Liu et al. | Rapid detection of Escherichia coli O157: H7 in milk, bread, and jelly by lac dye coloration‐based bidirectional lateral flow immunoassay strip | |
| CN107988401A (en) | The dry powdered LAMP quick detection kits of salmonella | |
| CN102816831A (en) | Kit for separating and identifying campylobacter rectus as well as preparation and application for kit | |
| CN106226536B (en) | A kind of position phase polymorphism test method of salmonella H antigens | |
| Li et al. | Real-time and visual detection of viable Salmonella in milk from remote pasture via IMS-LAMP-NALFS | |
| Oleksy et al. | Capsular polysaccharides of Lactobacillus spp.: theoretical and practical aspects of simple visualization methods | |
| CN107988327A (en) | The dry powdered LAMP quick detection kits of Shigella | |
| CN102071244A (en) | Method for accurately detecting toxicity of water quality by using photobacterium toxicity test | |
| Sun et al. | The adsorption of 2-amino-1-methyl-6-phenyl-imidazolium [4, 5-B] pyridine (PhIP) by lactic acid bacteria 37X-15 and its peptidoglycan | |
| CN106591483A (en) | Method for rapidly detecting pseudomonas aeruginosa in textile | |
| CN106544436A (en) | A kind of method of Salmonella in quick detection textile | |
| Arefin et al. | Development of slide agglutination test for the rapid diagnosis of Mycoplasma infection in the chicken | |
| CN104561234A (en) | Antibiotic residue detection kit and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |