JP2009534032A - Δ１７デサチュラーゼおよび多価不飽和脂肪酸の製造におけるそれらの使用 - Google Patents

Δ１７デサチュラーゼおよび多価不飽和脂肪酸の製造におけるそれらの使用 Download PDF

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Publication number
JP2009534032A
JP2009534032A JP2009506580A JP2009506580A JP2009534032A JP 2009534032 A JP2009534032 A JP 2009534032A JP 2009506580 A JP2009506580 A JP 2009506580A JP 2009506580 A JP2009506580 A JP 2009506580A JP 2009534032 A JP2009534032 A JP 2009534032A
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Prior art keywords
desaturase
seq
polypeptide
nucleic acid
gene
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Ceased
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JP2009506580A
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Japanese (ja)
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JP2009534032A5 (enExample
Inventor
ジシオン・シュー
クイン・クン・ズュー
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EIDP Inc
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EI Du Pont de Nemours and Co
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Publication of JP2009534032A publication Critical patent/JP2009534032A/ja
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Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6432—Eicosapentaenoic acids [EPA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0083—Miscellaneous (1.14.99)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0083—Miscellaneous (1.14.99)
    • C12N9/0087—Steroid 21-monooxygenase (1.14.99.10)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6472—Glycerides containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nutrition Science (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Fats And Perfumes (AREA)
JP2009506580A 2006-04-20 2007-04-19 Δ１７デサチュラーゼおよび多価不飽和脂肪酸の製造におけるそれらの使用 Ceased JP2009534032A (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US79357506P 2006-04-20 2006-04-20
PCT/US2007/009572 WO2007123999A2 (en) 2006-04-20 2007-04-19 Δ17 desaturases and their use in making polyunsaturated fatty acids

Publications (2)

Publication Number Publication Date
JP2009534032A true JP2009534032A (ja) 2009-09-24
JP2009534032A5 JP2009534032A5 (enExample) 2010-05-20

Family

ID=38625589

Family Applications (1)

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JP2009506580A Ceased JP2009534032A (ja) 2006-04-20 2007-04-19 Δ１７デサチュラーゼおよび多価不飽和脂肪酸の製造におけるそれらの使用

Country Status (9)

Country Link
US (1) US7465793B2 (enExample)
EP (1) EP2010648B1 (enExample)
JP (1) JP2009534032A (enExample)
CN (1) CN101646766B (enExample)
AT (1) ATE542892T1 (enExample)
AU (1) AU2007240741B2 (enExample)
DK (1) DK2010648T3 (enExample)
MX (1) MX2008013306A (enExample)
WO (1) WO2007123999A2 (enExample)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015029966A1 (ja) 2013-08-27 2015-03-05 国立大学法人京都大学 ω３不飽和脂肪酸酵素およびエイコサペンタエン酸の製造方法
WO2016104607A1 (ja) * 2014-12-25 2016-06-30 国立大学法人京都大学 新規ω３不飽和脂肪酸酵素およびエイコサペンタエン酸の製造方法

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2659993C (en) * 2006-08-24 2016-01-05 Basf Plant Science Gmbh Isolation and characterization of a novel pythium omega 3 desaturase with specificity to all omega 6 fatty acids longer than 18 carbon chains
WO2008054565A1 (en) * 2006-10-30 2008-05-08 E. I. Du Pont De Nemours And Company Δ17 desaturase and its use in making polyunsaturated fatty acids
US7709239B2 (en) * 2006-12-07 2010-05-04 E.I. Du Pont De Nemours And Company Mutant Δ8 desaturase genes engineered by targeted mutagenesis and their use in making polyunsaturated fatty acids
US8119860B2 (en) * 2007-04-16 2012-02-21 E. I. Du Pont De Nemours And Company Delta-9 elongases and their use in making polyunsaturated fatty acids
EP2337791B1 (en) * 2008-10-14 2013-07-24 Monsanto Technology LLC Utilization of fatty acid desaturases from hemiselmis spp
DE112009003708T5 (de) * 2008-12-12 2012-09-13 Basf Plant Science Gmbh Desaturasen und Verfahren zur Herstellung mehrfach ungesättigter Fettsäuren in transgenenOrganismen
WO2012027676A1 (en) * 2010-08-26 2012-03-01 E. I. Du Pont De Nemours And Company Mutant delta-9 elongases and their use in making polyunsaturated fatty acids
WO2017097218A1 (zh) * 2015-12-09 2017-06-15 江南大学 来源于寄生疫霉的用于合成多不饱和脂肪酸的ω-3脂肪酸脱饱和酶、含所述脂肪酸脱饱和酶的载体、重组微生物及其应用
CN105368851B (zh) * 2015-12-09 2019-05-07 江南大学 一种来源于寄生疫霉的ω-3脱饱和酶、含所述脱饱和酶的载体、重组微生物及其应用
CN106916796B (zh) * 2015-12-28 2021-06-29 丰益(上海)生物技术研发中心有限公司 裂殖壶菌delta-12脂肪酸脱饱和酶相关序列及其应用

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005083053A2 (de) * 2004-02-27 2005-09-09 Basf Plant Science Gmbh Verfahren zur herstellung von ungesättigten omega-3-fettsäuren in transgenen organismen
WO2005083093A2 (de) * 2004-02-27 2005-09-09 Basf Plant Science Gmbh Verfahren zur herstellung mehrfach ungesättigter fettsäuren in transgenen pflanzen

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US7008664B1 (en) * 1998-06-11 2006-03-07 E. I. Du Pont De Nemours And Company Method for improving the carcass quality of an animal
US6635451B2 (en) 2001-01-25 2003-10-21 Abbott Laboratories Desaturase genes and uses thereof
US7238482B2 (en) 2003-05-07 2007-07-03 E. I. Du Pont De Nemours And Company Production of polyunsaturated fatty acids in oleaginous yeasts
US7125672B2 (en) 2003-05-07 2006-10-24 E. I. Du Pont De Nemours And Company Codon-optimized genes for the production of polyunsaturated fatty acids in oleaginous yeasts
PT2172536T (pt) 2003-08-01 2016-08-17 Basf Plant Science Gmbh Processo para a produção de ácidos gordos poli-insaturados em organismos transgénicos
US20050132441A1 (en) 2003-11-12 2005-06-16 Damude Howard G. Delta15 desaturases suitable for altering levels of polyunsaturated fatty acids in oilseed plants and oleaginous yeast
US7192762B2 (en) 2004-11-04 2007-03-20 E. I. Du Pont De Nemours And Company Mortierella alpina glycerol-3-phosphate o-acyltransferase for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms
US7550286B2 (en) 2004-11-04 2009-06-23 E. I. Du Pont De Nemours And Company Docosahexaenoic acid producing strains of Yarrowia lipolytica
US7189559B2 (en) 2004-11-04 2007-03-13 E. I. Du Pont De Nemours And Company Mortierella alpina lysophosphatidic acid acyltransferase homolog for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms
DE102005013779A1 (de) 2005-03-22 2006-09-28 Basf Plant Science Gmbh Verfahren zur Herstellung von mehrfach ungesättigten C20- und C22-Fettsäuren mit mindestens vier Doppelbindungen in transgenen Pflanzen

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005083053A2 (de) * 2004-02-27 2005-09-09 Basf Plant Science Gmbh Verfahren zur herstellung von ungesättigten omega-3-fettsäuren in transgenen organismen
WO2005083093A2 (de) * 2004-02-27 2005-09-09 Basf Plant Science Gmbh Verfahren zur herstellung mehrfach ungesättigter fettsäuren in transgenen pflanzen

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
JPN6012027998; Ｙｅａｓｔ Ｖｏｌ．１８, 2001, ｐ．９７-１１３ *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015029966A1 (ja) 2013-08-27 2015-03-05 国立大学法人京都大学 ω３不飽和脂肪酸酵素およびエイコサペンタエン酸の製造方法
WO2016104607A1 (ja) * 2014-12-25 2016-06-30 国立大学法人京都大学 新規ω３不飽和脂肪酸酵素およびエイコサペンタエン酸の製造方法
JP6026709B1 (ja) * 2014-12-25 2016-11-16 国立大学法人京都大学 新規ω３不飽和脂肪酸酵素およびエイコサペンタエン酸の製造方法
US10538793B2 (en) 2014-12-25 2020-01-21 Kyoto University ω3 fatty-acid desaturase and method for producing eicosapentaenoic acid

Also Published As

Publication number Publication date
CN101646766B (zh) 2013-03-27
US20070249026A1 (en) 2007-10-25
AU2007240741B2 (en) 2013-01-31
CN101646766A (zh) 2010-02-10
MX2008013306A (es) 2008-10-27
AU2007240741A1 (en) 2007-11-01
WO2007123999A2 (en) 2007-11-01
ATE542892T1 (de) 2012-02-15
US7465793B2 (en) 2008-12-16
DK2010648T3 (da) 2012-05-21
WO2007123999A3 (en) 2008-04-24
EP2010648B1 (en) 2012-01-25
EP2010648A2 (en) 2009-01-07

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