JP2009509533A - 包括的な転写機構エンジニアリング - Google Patents
包括的な転写機構エンジニアリング Download PDFInfo
- Publication number
- JP2009509533A JP2009509533A JP2008533553A JP2008533553A JP2009509533A JP 2009509533 A JP2009509533 A JP 2009509533A JP 2008533553 A JP2008533553 A JP 2008533553A JP 2008533553 A JP2008533553 A JP 2008533553A JP 2009509533 A JP2009509533 A JP 2009509533A
- Authority
- JP
- Japan
- Prior art keywords
- cell
- nucleic acid
- gene
- cells
- transcription machinery
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000013518 transcription Methods 0.000 title claims abstract description 255
- 230000035897 transcription Effects 0.000 title claims abstract description 255
- 230000007246 mechanism Effects 0.000 title claims description 41
- 210000004027 cell Anatomy 0.000 claims description 341
- 108090000623 proteins and genes Proteins 0.000 claims description 207
- 238000000034 method Methods 0.000 claims description 186
- 150000007523 nucleic acids Chemical class 0.000 claims description 162
- 102000039446 nucleic acids Human genes 0.000 claims description 161
- 108020004707 nucleic acids Proteins 0.000 claims description 161
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical group CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 132
- 230000035772 mutation Effects 0.000 claims description 129
- 230000001965 increasing effect Effects 0.000 claims description 76
- 230000014509 gene expression Effects 0.000 claims description 65
- 102000004169 proteins and genes Human genes 0.000 claims description 56
- 238000004519 manufacturing process Methods 0.000 claims description 48
- FJKROLUGYXJWQN-UHFFFAOYSA-N 4-hydroxybenzoic acid Chemical compound OC(=O)C1=CC=C(O)C=C1 FJKROLUGYXJWQN-UHFFFAOYSA-N 0.000 claims description 46
- 230000012010 growth Effects 0.000 claims description 44
- 239000002207 metabolite Substances 0.000 claims description 42
- UPYKUZBSLRQECL-UKMVMLAPSA-N Lycopene Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1C(=C)CCCC1(C)C)C=CC=C(/C)C=CC2C(=C)CCCC2(C)C UPYKUZBSLRQECL-UKMVMLAPSA-N 0.000 claims description 41
- JEVVKJMRZMXFBT-XWDZUXABSA-N Lycophyll Natural products OC/C(=C/CC/C(=C\C=C\C(=C/C=C/C(=C\C=C\C=C(/C=C/C=C(\C=C\C=C(/CC/C=C(/CO)\C)\C)/C)\C)/C)\C)/C)/C JEVVKJMRZMXFBT-XWDZUXABSA-N 0.000 claims description 41
- OAIJSZIZWZSQBC-GYZMGTAESA-N lycopene Chemical compound CC(C)=CCC\C(C)=C\C=C\C(\C)=C\C=C\C(\C)=C\C=C\C=C(/C)\C=C\C=C(/C)\C=C\C=C(/C)CCC=C(C)C OAIJSZIZWZSQBC-GYZMGTAESA-N 0.000 claims description 41
- 229960004999 lycopene Drugs 0.000 claims description 41
- 235000012661 lycopene Nutrition 0.000 claims description 41
- 239000001751 lycopene Substances 0.000 claims description 41
- ZCIHMQAPACOQHT-ZGMPDRQDSA-N trans-isorenieratene Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/c1c(C)ccc(C)c1C)C=CC=C(/C)C=Cc2c(C)ccc(C)c2C ZCIHMQAPACOQHT-ZGMPDRQDSA-N 0.000 claims description 41
- 210000003527 eukaryotic cell Anatomy 0.000 claims description 33
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 claims description 27
- 229920000331 Polyhydroxybutyrate Polymers 0.000 claims description 25
- 239000005015 poly(hydroxybutyrate) Substances 0.000 claims description 25
- 229940090248 4-hydroxybenzoic acid Drugs 0.000 claims description 23
- 230000001580 bacterial effect Effects 0.000 claims description 21
- 101150102864 rpoD gene Proteins 0.000 claims description 21
- 241000894007 species Species 0.000 claims description 20
- 102000006467 TATA-Box Binding Protein Human genes 0.000 claims description 17
- 108010044281 TATA-Box Binding Protein Proteins 0.000 claims description 17
- 239000013604 expression vector Substances 0.000 claims description 17
- 101100091878 Plasmodium falciparum (isolate 3D7) rpoC2 gene Proteins 0.000 claims description 15
- 101150029016 rpo3 gene Proteins 0.000 claims description 15
- 101150117326 sigA gene Proteins 0.000 claims description 15
- 230000008859 change Effects 0.000 claims description 13
- 238000012258 culturing Methods 0.000 claims description 13
- 210000004962 mammalian cell Anatomy 0.000 claims description 12
- 230000022532 regulation of transcription, DNA-dependent Effects 0.000 claims description 12
- 239000002699 waste material Substances 0.000 claims description 12
- 210000005253 yeast cell Anatomy 0.000 claims description 12
- 108010009460 RNA Polymerase II Proteins 0.000 claims description 11
- 102000009572 RNA Polymerase II Human genes 0.000 claims description 11
- 230000003115 biocidal effect Effects 0.000 claims description 11
- 210000003463 organelle Anatomy 0.000 claims description 11
- 210000001236 prokaryotic cell Anatomy 0.000 claims description 11
- 231100000331 toxic Toxicity 0.000 claims description 11
- 230000002588 toxic effect Effects 0.000 claims description 11
- 108010013845 RNA Polymerase I Proteins 0.000 claims description 10
- 102000017143 RNA Polymerase I Human genes 0.000 claims description 10
- 108010078067 RNA Polymerase III Proteins 0.000 claims description 10
- 102000014450 RNA Polymerase III Human genes 0.000 claims description 10
- 230000027455 binding Effects 0.000 claims description 10
- 238000012217 deletion Methods 0.000 claims description 10
- 230000037430 deletion Effects 0.000 claims description 10
- 210000000130 stem cell Anatomy 0.000 claims description 10
- 230000003247 decreasing effect Effects 0.000 claims description 9
- 239000003960 organic solvent Substances 0.000 claims description 9
- 101150106872 rpoH gene Proteins 0.000 claims description 9
- 241000238631 Hexapoda Species 0.000 claims description 8
- 102000006290 Transcription Factor TFIID Human genes 0.000 claims description 8
- 108010083268 Transcription Factor TFIID Proteins 0.000 claims description 8
- 238000003776 cleavage reaction Methods 0.000 claims description 8
- 230000008723 osmotic stress Effects 0.000 claims description 8
- 101150076849 rpoS gene Proteins 0.000 claims description 8
- 230000007017 scission Effects 0.000 claims description 8
- 230000001225 therapeutic effect Effects 0.000 claims description 8
- XDTMQSROBMDMFD-UHFFFAOYSA-N Cyclohexane Chemical compound C1CCCCC1 XDTMQSROBMDMFD-UHFFFAOYSA-N 0.000 claims description 7
- 102000003964 Histone deacetylase Human genes 0.000 claims description 7
- 108090000353 Histone deacetylase Proteins 0.000 claims description 7
- 230000002538 fungal effect Effects 0.000 claims description 7
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 7
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 7
- 101150034869 rpo5 gene Proteins 0.000 claims description 7
- 235000000346 sugar Nutrition 0.000 claims description 7
- 101100306508 Bacillus subtilis (strain 168) sigB gene Proteins 0.000 claims description 6
- 108010036115 Histone Methyltransferases Proteins 0.000 claims description 6
- 102000011787 Histone Methyltransferases Human genes 0.000 claims description 6
- 101150033071 RPO7 gene Proteins 0.000 claims description 6
- 101150017109 fliA gene Proteins 0.000 claims description 6
- 230000002503 metabolic effect Effects 0.000 claims description 6
- 238000002493 microarray Methods 0.000 claims description 6
- 229920001184 polypeptide Polymers 0.000 claims description 6
- 230000002829 reductive effect Effects 0.000 claims description 6
- 101150067683 rpo10 gene Proteins 0.000 claims description 6
- 101150084116 rpo4 gene Proteins 0.000 claims description 6
- 101150040886 rpoE gene Proteins 0.000 claims description 6
- 101150011750 rpoN gene Proteins 0.000 claims description 6
- 101150077142 sigH gene Proteins 0.000 claims description 6
- 230000002103 transcriptional effect Effects 0.000 claims description 6
- 108050007599 Anti-sigma factor Proteins 0.000 claims description 5
- 101100334569 Escherichia coli (strain K12) fecI gene Proteins 0.000 claims description 5
- 108090000246 Histone acetyltransferases Proteins 0.000 claims description 5
- 102000003893 Histone acetyltransferases Human genes 0.000 claims description 5
- 102000008394 Immunoglobulin Fragments Human genes 0.000 claims description 5
- 108010021625 Immunoglobulin Fragments Proteins 0.000 claims description 5
- 108020004459 Small interfering RNA Proteins 0.000 claims description 5
- 210000003763 chloroplast Anatomy 0.000 claims description 5
- 239000002920 hazardous waste Substances 0.000 claims description 5
- 239000002904 solvent Substances 0.000 claims description 5
- 210000001519 tissue Anatomy 0.000 claims description 5
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 claims description 4
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 claims description 4
- 230000006907 apoptotic process Effects 0.000 claims description 4
- 238000004113 cell culture Methods 0.000 claims description 4
- 201000010099 disease Diseases 0.000 claims description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 4
- 210000003470 mitochondria Anatomy 0.000 claims description 4
- 239000004094 surface-active agent Substances 0.000 claims description 4
- 101150098914 GAL11 gene Proteins 0.000 claims description 3
- 206010035148 Plague Diseases 0.000 claims description 3
- 108700040121 Protein Methyltransferases Proteins 0.000 claims description 3
- 102000055027 Protein Methyltransferases Human genes 0.000 claims description 3
- 101150109831 SIN4 gene Proteins 0.000 claims description 3
- 101150016929 SWI1 gene Proteins 0.000 claims description 3
- 101100533773 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) SNF6 gene Proteins 0.000 claims description 3
- 241000607479 Yersinia pestis Species 0.000 claims description 3
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 claims description 3
- 235000013399 edible fruits Nutrition 0.000 claims description 3
- 238000012203 high throughput assay Methods 0.000 claims description 3
- 239000000543 intermediate Substances 0.000 claims description 3
- 239000000463 material Substances 0.000 claims description 3
- 239000000758 substrate Substances 0.000 claims description 3
- 108010013043 Acetylesterase Proteins 0.000 claims description 2
- 101150009285 HRS1 gene Proteins 0.000 claims description 2
- 101150113148 MED2 gene Proteins 0.000 claims description 2
- 101150031895 PAF1 gene Proteins 0.000 claims description 2
- 101150102885 RGR1 gene Proteins 0.000 claims description 2
- 101150112782 SNF2 gene Proteins 0.000 claims description 2
- 101100422887 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) SWI1 gene Proteins 0.000 claims description 2
- 230000002194 synthesizing effect Effects 0.000 claims description 2
- 102000018068 TATA-Binding Protein Associated Factors Human genes 0.000 claims 4
- 108010091120 TATA-Binding Protein Associated Factors Proteins 0.000 claims 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Natural products CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims 2
- 101100259732 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) TAF10 gene Proteins 0.000 claims 2
- 125000000218 acetic acid group Chemical group C(C)(=O)* 0.000 claims 1
- 230000003252 repetitive effect Effects 0.000 claims 1
- 230000001976 improved effect Effects 0.000 abstract description 14
- KWGKDLIKAYFUFQ-UHFFFAOYSA-M lithium chloride Chemical compound [Li+].[Cl-] KWGKDLIKAYFUFQ-UHFFFAOYSA-M 0.000 description 36
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 35
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 33
- 230000006872 improvement Effects 0.000 description 29
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 27
- 239000008103 glucose Substances 0.000 description 26
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 description 22
- 241000588724 Escherichia coli Species 0.000 description 22
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 22
- 239000000047 product Substances 0.000 description 21
- 239000002609 medium Substances 0.000 description 20
- 239000013612 plasmid Substances 0.000 description 20
- 239000013598 vector Substances 0.000 description 19
- 238000002703 mutagenesis Methods 0.000 description 18
- 231100000350 mutagenesis Toxicity 0.000 description 18
- 239000002773 nucleotide Substances 0.000 description 18
- 125000003729 nucleotide group Chemical group 0.000 description 18
- 108020004414 DNA Proteins 0.000 description 15
- 101000840577 Homo sapiens Insulin-like growth factor-binding protein 7 Proteins 0.000 description 14
- 102100029228 Insulin-like growth factor-binding protein 7 Human genes 0.000 description 14
- 241000894006 Bacteria Species 0.000 description 13
- 238000004458 analytical method Methods 0.000 description 13
- 239000012634 fragment Substances 0.000 description 11
- 238000002864 sequence alignment Methods 0.000 description 11
- 125000003275 alpha amino acid group Chemical group 0.000 description 10
- 230000001413 cellular effect Effects 0.000 description 10
- 108090000951 RNA polymerase sigma 70 Proteins 0.000 description 9
- 238000012300 Sequence Analysis Methods 0.000 description 9
- 102000017354 TATA-Box binding protein-like Human genes 0.000 description 9
- 108050005399 TATA-Box binding protein-like Proteins 0.000 description 9
- 238000002474 experimental method Methods 0.000 description 9
- 238000000855 fermentation Methods 0.000 description 9
- 230000004151 fermentation Effects 0.000 description 9
- 244000005700 microbiome Species 0.000 description 9
- 230000001105 regulatory effect Effects 0.000 description 9
- 241000196324 Embryophyta Species 0.000 description 8
- 238000003556 assay Methods 0.000 description 8
- 230000000694 effects Effects 0.000 description 8
- 108700039691 Genetic Promoter Regions Proteins 0.000 description 7
- 101100098950 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) SPT15 gene Proteins 0.000 description 7
- 230000004048 modification Effects 0.000 description 7
- 238000012986 modification Methods 0.000 description 7
- VOFUROIFQGPCGE-UHFFFAOYSA-N nile red Chemical compound C1=CC=C2C3=NC4=CC=C(N(CC)CC)C=C4OC3=CC(=O)C2=C1 VOFUROIFQGPCGE-UHFFFAOYSA-N 0.000 description 7
- 108091026890 Coding region Proteins 0.000 description 6
- 108091028043 Nucleic acid sequence Proteins 0.000 description 6
- 239000003623 enhancer Substances 0.000 description 6
- 230000002068 genetic effect Effects 0.000 description 6
- 239000000203 mixture Substances 0.000 description 6
- 230000008569 process Effects 0.000 description 6
- 230000010076 replication Effects 0.000 description 6
- 230000035939 shock Effects 0.000 description 6
- 238000010186 staining Methods 0.000 description 6
- 239000011550 stock solution Substances 0.000 description 6
- 102000004163 DNA-directed RNA polymerases Human genes 0.000 description 5
- 108090000626 DNA-directed RNA polymerases Proteins 0.000 description 5
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 5
- 102000004190 Enzymes Human genes 0.000 description 5
- 108090000790 Enzymes Proteins 0.000 description 5
- 229930006000 Sucrose Natural products 0.000 description 5
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 5
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 5
- 230000006870 function Effects 0.000 description 5
- 238000012239 gene modification Methods 0.000 description 5
- 238000005457 optimization Methods 0.000 description 5
- 238000012261 overproduction Methods 0.000 description 5
- 239000005720 sucrose Substances 0.000 description 5
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 4
- 241000620209 Escherichia coli DH5[alpha] Species 0.000 description 4
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 4
- 239000003242 anti bacterial agent Substances 0.000 description 4
- 229940088710 antibiotic agent Drugs 0.000 description 4
- 230000015572 biosynthetic process Effects 0.000 description 4
- 229960005091 chloramphenicol Drugs 0.000 description 4
- WIIZWVCIJKGZOK-RKDXNWHRSA-N chloramphenicol Chemical compound ClC(Cl)C(=O)N[C@H](CO)[C@H](O)C1=CC=C([N+]([O-])=O)C=C1 WIIZWVCIJKGZOK-RKDXNWHRSA-N 0.000 description 4
- YMFBFFPJRABBPE-BTVCFUMJSA-N ethanol;(2r,3s,4r,5r)-2,3,4,5,6-pentahydroxyhexanal Chemical compound CCO.OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C=O YMFBFFPJRABBPE-BTVCFUMJSA-N 0.000 description 4
- 230000002401 inhibitory effect Effects 0.000 description 4
- 229960000210 nalidixic acid Drugs 0.000 description 4
- MHWLWQUZZRMNGJ-UHFFFAOYSA-N nalidixic acid Chemical compound C1=C(C)N=C2N(CC)C=C(C(O)=O)C(=O)C2=C1 MHWLWQUZZRMNGJ-UHFFFAOYSA-N 0.000 description 4
- 239000008188 pellet Substances 0.000 description 4
- 230000009467 reduction Effects 0.000 description 4
- 238000012216 screening Methods 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 238000006467 substitution reaction Methods 0.000 description 4
- 238000003786 synthesis reaction Methods 0.000 description 4
- 239000003053 toxin Substances 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 108700028146 Genetic Enhancer Elements Proteins 0.000 description 3
- 108010004889 Heat-Shock Proteins Proteins 0.000 description 3
- 108010025076 Holoenzymes Proteins 0.000 description 3
- 241000282414 Homo sapiens Species 0.000 description 3
- 241000187747 Streptomyces Species 0.000 description 3
- 102000040945 Transcription factor Human genes 0.000 description 3
- 108091023040 Transcription factor Proteins 0.000 description 3
- 230000002411 adverse Effects 0.000 description 3
- 230000004075 alteration Effects 0.000 description 3
- 229940024606 amino acid Drugs 0.000 description 3
- 150000001413 amino acids Chemical class 0.000 description 3
- 230000003698 anagen phase Effects 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 239000000872 buffer Substances 0.000 description 3
- 230000010261 cell growth Effects 0.000 description 3
- 238000010276 construction Methods 0.000 description 3
- 230000002939 deleterious effect Effects 0.000 description 3
- 238000000684 flow cytometry Methods 0.000 description 3
- 238000003209 gene knockout Methods 0.000 description 3
- 229930027917 kanamycin Natural products 0.000 description 3
- 229960000318 kanamycin Drugs 0.000 description 3
- SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 3
- 229930182823 kanamycin A Natural products 0.000 description 3
- 239000003550 marker Substances 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 230000004044 response Effects 0.000 description 3
- 239000000523 sample Substances 0.000 description 3
- 238000010561 standard procedure Methods 0.000 description 3
- 230000004083 survival effect Effects 0.000 description 3
- 230000037426 transcriptional repression Effects 0.000 description 3
- 230000014616 translation Effects 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 230000004568 DNA-binding Effects 0.000 description 2
- 241000252212 Danio rerio Species 0.000 description 2
- 241000255601 Drosophila melanogaster Species 0.000 description 2
- 101100341136 Escherichia coli (strain K12) iraD gene Proteins 0.000 description 2
- 102000002812 Heat-Shock Proteins Human genes 0.000 description 2
- 102100022846 Histone acetyltransferase KAT2B Human genes 0.000 description 2
- 102100021455 Histone deacetylase 3 Human genes 0.000 description 2
- 101001047006 Homo sapiens Histone acetyltransferase KAT2B Proteins 0.000 description 2
- 101001035011 Homo sapiens Histone deacetylase 2 Proteins 0.000 description 2
- 101000899282 Homo sapiens Histone deacetylase 3 Proteins 0.000 description 2
- 101001032118 Homo sapiens Histone deacetylase 8 Proteins 0.000 description 2
- 241000701044 Human gammaherpesvirus 4 Species 0.000 description 2
- 241000124008 Mammalia Species 0.000 description 2
- 241000244206 Nematoda Species 0.000 description 2
- 238000010222 PCR analysis Methods 0.000 description 2
- 108010063499 Sigma Factor Proteins 0.000 description 2
- 241000192584 Synechocystis Species 0.000 description 2
- 241000589634 Xanthomonas Species 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 210000001106 artificial yeast chromosome Anatomy 0.000 description 2
- 238000007630 basic procedure Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- MYSWGUAQZAJSOK-UHFFFAOYSA-N ciprofloxacin Chemical compound C12=CC(N3CCNCC3)=C(F)C=C2C(=O)C(C(=O)O)=CN1C1CC1 MYSWGUAQZAJSOK-UHFFFAOYSA-N 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 239000013078 crystal Substances 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 239000008367 deionised water Substances 0.000 description 2
- 229910021641 deionized water Inorganic materials 0.000 description 2
- 238000009826 distribution Methods 0.000 description 2
- 108091006047 fluorescent proteins Proteins 0.000 description 2
- 102000034287 fluorescent proteins Human genes 0.000 description 2
- 125000002485 formyl group Chemical group [H]C(*)=O 0.000 description 2
- 230000004927 fusion Effects 0.000 description 2
- 239000006481 glucose medium Substances 0.000 description 2
- 230000005484 gravity Effects 0.000 description 2
- 239000005090 green fluorescent protein Substances 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 230000006698 induction Effects 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 238000002955 isolation Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 230000004060 metabolic process Effects 0.000 description 2
- 238000010369 molecular cloning Methods 0.000 description 2
- 230000000869 mutational effect Effects 0.000 description 2
- 230000002018 overexpression Effects 0.000 description 2
- 230000006916 protein interaction Effects 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 108091008146 restriction endonucleases Proteins 0.000 description 2
- 238000002741 site-directed mutagenesis Methods 0.000 description 2
- 239000010891 toxic waste Substances 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 230000009466 transformation Effects 0.000 description 2
- PKAUICCNAWQPAU-UHFFFAOYSA-N 2-(4-chloro-2-methylphenoxy)acetic acid;n-methylmethanamine Chemical compound CNC.CC1=CC(Cl)=CC=C1OCC(O)=O PKAUICCNAWQPAU-UHFFFAOYSA-N 0.000 description 1
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 description 1
- UHPMCKVQTMMPCG-UHFFFAOYSA-N 5,8-dihydroxy-2-methoxy-6-methyl-7-(2-oxopropyl)naphthalene-1,4-dione Chemical compound CC1=C(CC(C)=O)C(O)=C2C(=O)C(OC)=CC(=O)C2=C1O UHPMCKVQTMMPCG-UHFFFAOYSA-N 0.000 description 1
- OPIFSICVWOWJMJ-AEOCFKNESA-N 5-bromo-4-chloro-3-indolyl beta-D-galactoside Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1OC1=CNC2=CC=C(Br)C(Cl)=C12 OPIFSICVWOWJMJ-AEOCFKNESA-N 0.000 description 1
- 101710187795 60S ribosomal protein L15 Proteins 0.000 description 1
- 241000589220 Acetobacter Species 0.000 description 1
- 241001019659 Acremonium <Plectosphaerellaceae> Species 0.000 description 1
- 102000007469 Actins Human genes 0.000 description 1
- 108010085238 Actins Proteins 0.000 description 1
- 241000607534 Aeromonas Species 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 241000589158 Agrobacterium Species 0.000 description 1
- 241000588986 Alcaligenes Species 0.000 description 1
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 1
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 1
- 241000190857 Allochromatium vinosum Species 0.000 description 1
- 241001136561 Allomyces Species 0.000 description 1
- 241000219195 Arabidopsis thaliana Species 0.000 description 1
- 101100444285 Arabidopsis thaliana DYAD gene Proteins 0.000 description 1
- 101100123574 Arabidopsis thaliana HDA19 gene Proteins 0.000 description 1
- 101100339677 Arabidopsis thaliana HRS1 gene Proteins 0.000 description 1
- 101000634115 Arabidopsis thaliana RNA polymerase sigma factor sigE, chloroplastic/mitochondrial Proteins 0.000 description 1
- 241000203069 Archaea Species 0.000 description 1
- 241000205046 Archaeoglobus Species 0.000 description 1
- 241000186063 Arthrobacter Species 0.000 description 1
- 241000228212 Aspergillus Species 0.000 description 1
- 241000589151 Azotobacter Species 0.000 description 1
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 108700003860 Bacterial Genes Proteins 0.000 description 1
- 108010023063 Bacto-peptone Proteins 0.000 description 1
- 102100026189 Beta-galactosidase Human genes 0.000 description 1
- 101100454433 Biomphalaria glabrata BG01 gene Proteins 0.000 description 1
- 241000255789 Bombyx mori Species 0.000 description 1
- 241001465180 Botrytis Species 0.000 description 1
- 241000244203 Caenorhabditis elegans Species 0.000 description 1
- 101000687585 Caenorhabditis elegans REST corepressor spr-1 Proteins 0.000 description 1
- 241000222120 Candida <Saccharomycetales> Species 0.000 description 1
- 241000282465 Canis Species 0.000 description 1
- 102100028633 Cdc42-interacting protein 4 Human genes 0.000 description 1
- 241000588881 Chromobacterium Species 0.000 description 1
- 241000588923 Citrobacter Species 0.000 description 1
- 241000193403 Clostridium Species 0.000 description 1
- 241000589519 Comamonas Species 0.000 description 1
- 241000186216 Corynebacterium Species 0.000 description 1
- 241001528539 Cupriavidus necator Species 0.000 description 1
- 241000701022 Cytomegalovirus Species 0.000 description 1
- 108010009540 DNA (Cytosine-5-)-Methyltransferase 1 Proteins 0.000 description 1
- 102100036279 DNA (cytosine-5)-methyltransferase 1 Human genes 0.000 description 1
- 102100024812 DNA (cytosine-5)-methyltransferase 3A Human genes 0.000 description 1
- 108050002829 DNA (cytosine-5)-methyltransferase 3A Proteins 0.000 description 1
- 102000052510 DNA-Binding Proteins Human genes 0.000 description 1
- 108700020911 DNA-Binding Proteins Proteins 0.000 description 1
- 241000235035 Debaryomyces Species 0.000 description 1
- 101000687583 Drosophila melanogaster REST corepressor Proteins 0.000 description 1
- 241000257465 Echinoidea Species 0.000 description 1
- 102100031780 Endonuclease Human genes 0.000 description 1
- 108010042407 Endonucleases Proteins 0.000 description 1
- 241000588722 Escherichia Species 0.000 description 1
- 241000531184 Ferroglobus Species 0.000 description 1
- 241000589565 Flavobacterium Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 241000223218 Fusarium Species 0.000 description 1
- 230000005526 G1 to G0 transition Effects 0.000 description 1
- 241000626621 Geobacillus Species 0.000 description 1
- 241001135750 Geobacter Species 0.000 description 1
- 241000589236 Gluconobacter Species 0.000 description 1
- 108010043121 Green Fluorescent Proteins Proteins 0.000 description 1
- 102000004144 Green Fluorescent Proteins Human genes 0.000 description 1
- 101150083200 HDA1 gene Proteins 0.000 description 1
- 241000205062 Halobacterium Species 0.000 description 1
- 102100027685 Hemoglobin subunit alpha Human genes 0.000 description 1
- 102100022893 Histone acetyltransferase KAT5 Human genes 0.000 description 1
- 101710116149 Histone acetyltransferase KAT5 Proteins 0.000 description 1
- 102100039996 Histone deacetylase 1 Human genes 0.000 description 1
- 102100039999 Histone deacetylase 2 Human genes 0.000 description 1
- 102100038715 Histone deacetylase 8 Human genes 0.000 description 1
- 102100022103 Histone-lysine N-methyltransferase 2A Human genes 0.000 description 1
- 102100029768 Histone-lysine N-methyltransferase SETD1A Human genes 0.000 description 1
- 101001009007 Homo sapiens Hemoglobin subunit alpha Proteins 0.000 description 1
- 101001046967 Homo sapiens Histone acetyltransferase KAT2A Proteins 0.000 description 1
- 101001035024 Homo sapiens Histone deacetylase 1 Proteins 0.000 description 1
- 101001045846 Homo sapiens Histone-lysine N-methyltransferase 2A Proteins 0.000 description 1
- 101000865038 Homo sapiens Histone-lysine N-methyltransferase SETD1A Proteins 0.000 description 1
- 101001019104 Homo sapiens Mediator of RNA polymerase II transcription subunit 14 Proteins 0.000 description 1
- 101001013272 Homo sapiens Mediator of RNA polymerase II transcription subunit 29 Proteins 0.000 description 1
- 101000772905 Homo sapiens Polyubiquitin-B Proteins 0.000 description 1
- 101000702559 Homo sapiens Probable global transcription activator SNF2L2 Proteins 0.000 description 1
- 101000585534 Homo sapiens RNA polymerase II-associated factor 1 homolog Proteins 0.000 description 1
- 101000702545 Homo sapiens Transcription activator BRG1 Proteins 0.000 description 1
- 101100141719 Human cytomegalovirus (strain Merlin) RL13 gene Proteins 0.000 description 1
- 241000235649 Kluyveromyces Species 0.000 description 1
- AGPKZVBTJJNPAG-WHFBIAKZSA-N L-isoleucine Chemical compound CC[C@H](C)[C@H](N)C(O)=O AGPKZVBTJJNPAG-WHFBIAKZSA-N 0.000 description 1
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 1
- KZSNJWFQEVHDMF-BYPYZUCNSA-N L-valine Chemical compound CC(C)[C@H](N)C(O)=O KZSNJWFQEVHDMF-BYPYZUCNSA-N 0.000 description 1
- 241000186660 Lactobacillus Species 0.000 description 1
- 241000194036 Lactococcus Species 0.000 description 1
- 108060001084 Luciferase Proteins 0.000 description 1
- 239000005089 Luciferase Substances 0.000 description 1
- 239000006142 Luria-Bertani Agar Substances 0.000 description 1
- 241000721703 Lymantria dispar Species 0.000 description 1
- 241001344133 Magnaporthe Species 0.000 description 1
- 102100034820 Mediator of RNA polymerase II transcription subunit 14 Human genes 0.000 description 1
- 102100029668 Mediator of RNA polymerase II transcription subunit 29 Human genes 0.000 description 1
- 241000202974 Methanobacterium Species 0.000 description 1
- 241001074893 Methanococci Species 0.000 description 1
- 241001083901 Methanopyri Species 0.000 description 1
- 241000589323 Methylobacterium Species 0.000 description 1
- 241000589344 Methylomonas Species 0.000 description 1
- 102000016397 Methyltransferase Human genes 0.000 description 1
- 108060004795 Methyltransferase Proteins 0.000 description 1
- 108700011259 MicroRNAs Proteins 0.000 description 1
- 241001148170 Microlunatus Species 0.000 description 1
- 241000699660 Mus musculus Species 0.000 description 1
- 241000186359 Mycobacterium Species 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 241000221960 Neurospora Species 0.000 description 1
- 102100022935 Nuclear receptor corepressor 1 Human genes 0.000 description 1
- 101710153661 Nuclear receptor corepressor 1 Proteins 0.000 description 1
- 102100030569 Nuclear receptor corepressor 2 Human genes 0.000 description 1
- 101710153660 Nuclear receptor corepressor 2 Proteins 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- 241000520272 Pantoea Species 0.000 description 1
- 206010034133 Pathogen resistance Diseases 0.000 description 1
- 102100025516 Peroxisome biogenesis factor 2 Human genes 0.000 description 1
- 241000235648 Pichia Species 0.000 description 1
- 102100030432 Polyubiquitin-B Human genes 0.000 description 1
- 241000288906 Primates Species 0.000 description 1
- 108010076504 Protein Sorting Signals Proteins 0.000 description 1
- 241000589516 Pseudomonas Species 0.000 description 1
- 241000232299 Ralstonia Species 0.000 description 1
- 241000700157 Rattus norvegicus Species 0.000 description 1
- 108700008625 Reporter Genes Proteins 0.000 description 1
- 241000589180 Rhizobium Species 0.000 description 1
- 101100297400 Rhizobium meliloti (strain 1021) phaAB gene Proteins 0.000 description 1
- 241000235527 Rhizopus Species 0.000 description 1
- 241000316848 Rhodococcus <scale insect> Species 0.000 description 1
- 241000235070 Saccharomyces Species 0.000 description 1
- 101100129874 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) PGD1 gene Proteins 0.000 description 1
- 241000187560 Saccharopolyspora Species 0.000 description 1
- 241000235346 Schizosaccharomyces Species 0.000 description 1
- 101100129591 Schizosaccharomyces pombe (strain 972 / ATCC 24843) mcp6 gene Proteins 0.000 description 1
- 101100297422 Schizosaccharomyces pombe (strain 972 / ATCC 24843) phd1 gene Proteins 0.000 description 1
- 102100026842 Serine-pyruvate aminotransferase Human genes 0.000 description 1
- 241000607720 Serratia Species 0.000 description 1
- 241001135312 Sinorhizobium Species 0.000 description 1
- 241000221948 Sordaria Species 0.000 description 1
- 241000256251 Spodoptera frugiperda Species 0.000 description 1
- 241000122971 Stenotrophomonas Species 0.000 description 1
- 241000194017 Streptococcus Species 0.000 description 1
- 241000205101 Sulfolobus Species 0.000 description 1
- 241000228341 Talaromyces Species 0.000 description 1
- 239000004098 Tetracycline Substances 0.000 description 1
- 241001074959 Thermococci Species 0.000 description 1
- 241001074960 Thermoplasmata Species 0.000 description 1
- 241000589596 Thermus Species 0.000 description 1
- JZRWCGZRTZMZEH-UHFFFAOYSA-N Thiamine Natural products CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N JZRWCGZRTZMZEH-UHFFFAOYSA-N 0.000 description 1
- 102100031027 Transcription activator BRG1 Human genes 0.000 description 1
- 241000223259 Trichoderma Species 0.000 description 1
- 241000221566 Ustilago Species 0.000 description 1
- KZSNJWFQEVHDMF-UHFFFAOYSA-N Valine Natural products CC(C)C(N)C(O)=O KZSNJWFQEVHDMF-UHFFFAOYSA-N 0.000 description 1
- 241000269368 Xenopus laevis Species 0.000 description 1
- 150000001242 acetic acid derivatives Chemical class 0.000 description 1
- ZSLZBFCDCINBPY-ZSJPKINUSA-N acetyl-CoA Chemical compound O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(=O)OP(O)(=O)OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCSC(=O)C)O[C@H]1N1C2=NC=NC(N)=C2N=C1 ZSLZBFCDCINBPY-ZSJPKINUSA-N 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 150000001299 aldehydes Chemical class 0.000 description 1
- 125000000539 amino acid group Chemical group 0.000 description 1
- 229960000723 ampicillin Drugs 0.000 description 1
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 1
- 230000002424 anti-apoptotic effect Effects 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- XKRFYHLGVUSROY-UHFFFAOYSA-N argon Substances [Ar] XKRFYHLGVUSROY-UHFFFAOYSA-N 0.000 description 1
- 229910052786 argon Inorganic materials 0.000 description 1
- -1 argon ion Chemical class 0.000 description 1
- 210000004507 artificial chromosome Anatomy 0.000 description 1
- 108010005774 beta-Galactosidase Proteins 0.000 description 1
- 102000023732 binding proteins Human genes 0.000 description 1
- 108091008324 binding proteins Proteins 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 101150055766 cat gene Proteins 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 230000003197 catalytic effect Effects 0.000 description 1
- 230000004663 cell proliferation Effects 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
- 239000013611 chromosomal DNA Substances 0.000 description 1
- 210000000349 chromosome Anatomy 0.000 description 1
- 229960003405 ciprofloxacin Drugs 0.000 description 1
- NGSWKAQJJWESNS-UHFFFAOYSA-N cis-para-coumaric acid Natural products OC(=O)C=CC1=CC=C(O)C=C1 NGSWKAQJJWESNS-UHFFFAOYSA-N 0.000 description 1
- 239000013599 cloning vector Substances 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 210000004443 dendritic cell Anatomy 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 101150036810 eco gene Proteins 0.000 description 1
- 238000004520 electroporation Methods 0.000 description 1
- 210000002308 embryonic cell Anatomy 0.000 description 1
- 238000012407 engineering method Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000007717 exclusion Effects 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 101150001815 flgM gene Proteins 0.000 description 1
- 231100000221 frame shift mutation induction Toxicity 0.000 description 1
- 230000037433 frameshift Effects 0.000 description 1
- 230000004077 genetic alteration Effects 0.000 description 1
- 231100000118 genetic alteration Toxicity 0.000 description 1
- 230000005017 genetic modification Effects 0.000 description 1
- 235000013617 genetically modified food Nutrition 0.000 description 1
- 230000009229 glucose formation Effects 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- 230000001506 immunosuppresive effect Effects 0.000 description 1
- 238000011065 in-situ storage Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 230000000977 initiatory effect Effects 0.000 description 1
- 239000002917 insecticide Substances 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 229960000310 isoleucine Drugs 0.000 description 1
- AGPKZVBTJJNPAG-UHFFFAOYSA-N isoleucine Natural products CCC(C)C(N)C(O)=O AGPKZVBTJJNPAG-UHFFFAOYSA-N 0.000 description 1
- 238000012804 iterative process Methods 0.000 description 1
- 101150066555 lacZ gene Proteins 0.000 description 1
- 229940039696 lactobacillus Drugs 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 238000001638 lipofection Methods 0.000 description 1
- 238000011068 loading method Methods 0.000 description 1
- 230000001320 lysogenic effect Effects 0.000 description 1
- 230000002101 lytic effect Effects 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 230000010534 mechanism of action Effects 0.000 description 1
- 230000001404 mediated effect Effects 0.000 description 1
- 239000013028 medium composition Substances 0.000 description 1
- 108020004999 messenger RNA Proteins 0.000 description 1
- 238000012269 metabolic engineering Methods 0.000 description 1
- 239000002679 microRNA Substances 0.000 description 1
- 238000000520 microinjection Methods 0.000 description 1
- 230000002438 mitochondrial effect Effects 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 230000009456 molecular mechanism Effects 0.000 description 1
- 210000000663 muscle cell Anatomy 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 231100000989 no adverse effect Toxicity 0.000 description 1
- 230000005257 nucleotidylation Effects 0.000 description 1
- 210000000287 oocyte Anatomy 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 230000008823 permeabilization Effects 0.000 description 1
- 230000027086 plasmid maintenance Effects 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- FWLKYEAOOIPJRL-UHFFFAOYSA-N prop-1-yn-1-ol Chemical compound CC#CO FWLKYEAOOIPJRL-UHFFFAOYSA-N 0.000 description 1
- 108020001775 protein parts Proteins 0.000 description 1
- 210000001938 protoplast Anatomy 0.000 description 1
- LISFMEBWQUVKPJ-UHFFFAOYSA-N quinolin-2-ol Chemical compound C1=CC=C2NC(=O)C=CC2=C1 LISFMEBWQUVKPJ-UHFFFAOYSA-N 0.000 description 1
- 238000002708 random mutagenesis Methods 0.000 description 1
- 230000009711 regulatory function Effects 0.000 description 1
- 238000005067 remediation Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000028160 response to osmotic stress Effects 0.000 description 1
- 230000002441 reversible effect Effects 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000010845 search algorithm Methods 0.000 description 1
- 239000006152 selective media Substances 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
- 229940126586 small molecule drug Drugs 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000012289 standard assay Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 230000008685 targeting Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 229960002180 tetracycline Drugs 0.000 description 1
- 229930101283 tetracycline Natural products 0.000 description 1
- 235000019364 tetracycline Nutrition 0.000 description 1
- 150000003522 tetracyclines Chemical class 0.000 description 1
- KYMBYSLLVAOCFI-UHFFFAOYSA-N thiamine Chemical compound CC1=C(CCO)SCN1CC1=CN=C(C)N=C1N KYMBYSLLVAOCFI-UHFFFAOYSA-N 0.000 description 1
- 229960003495 thiamine Drugs 0.000 description 1
- 235000019157 thiamine Nutrition 0.000 description 1
- 239000011721 thiamine Substances 0.000 description 1
- 231100000167 toxic agent Toxicity 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
- 108700012359 toxins Proteins 0.000 description 1
- NGSWKAQJJWESNS-ZZXKWVIFSA-N trans-4-coumaric acid Chemical compound OC(=O)\C=C\C1=CC=C(O)C=C1 NGSWKAQJJWESNS-ZZXKWVIFSA-N 0.000 description 1
- 238000010361 transduction Methods 0.000 description 1
- 230000026683 transduction Effects 0.000 description 1
- 238000013519 translation Methods 0.000 description 1
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
- 238000010200 validation analysis Methods 0.000 description 1
- 239000004474 valine Substances 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
- 230000035899 viability Effects 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
- 239000007222 ypd medium Substances 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
- C07K14/245—Escherichia (G)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1079—Screening libraries by altering the phenotype or phenotypic trait of the host
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P23/00—Preparation of compounds containing a cyclohexene ring having an unsaturated side chain containing at least ten carbon atoms bound by conjugated double bonds, e.g. carotenes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
- C12P7/42—Hydroxy-carboxylic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/62—Carboxylic acid esters
- C12P7/625—Polyesters of hydroxy carboxylic acids
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Bioinformatics & Computational Biology (AREA)
- Gastroenterology & Hepatology (AREA)
- Mycology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Ecology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Processing Of Solid Wastes (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US11/238,096 US20070072194A1 (en) | 2005-09-28 | 2005-09-28 | Global transcription machinery engineering |
| US74831505P | 2005-12-07 | 2005-12-07 | |
| PCT/US2006/037597 WO2007038564A2 (en) | 2005-09-28 | 2006-09-28 | Global transcription machinery engineering |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2009509533A true JP2009509533A (ja) | 2009-03-12 |
| JP2009509533A5 JP2009509533A5 (enExample) | 2009-11-12 |
Family
ID=37891512
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2008533553A Pending JP2009509533A (ja) | 2005-09-28 | 2006-09-28 | 包括的な転写機構エンジニアリング |
Country Status (3)
| Country | Link |
|---|---|
| EP (1) | EP1943343A2 (enExample) |
| JP (1) | JP2009509533A (enExample) |
| WO (1) | WO2007038564A2 (enExample) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2016521763A (ja) * | 2013-06-24 | 2016-07-25 | シージェイ チェイルジェダン コーポレーションCj Cheiljedang Corporation | L−スレオニン産生微生物およびこれを用いたl−スレオニンの産生方法 |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2010512147A (ja) * | 2006-12-07 | 2010-04-22 | マサチューセッツ インスティテュート オブ テクノロジー | 包括的転写マシナリーエンジニアリング |
| WO2009009084A2 (en) * | 2007-07-12 | 2009-01-15 | Massachusetts Institute Of Technology | Transcriptional engineering of lactobacillus |
| WO2009025761A2 (en) | 2007-08-17 | 2009-02-26 | Massachusetts Institute Of Technology | Methods for identifying bacterial strains that produce l-tyrosine |
| US20100330614A1 (en) * | 2007-11-06 | 2010-12-30 | Massachusetts Institute Of Technology | Global transcription machinery engineering targeting the rnap alpha subunit (rpoa) |
| WO2011159629A2 (en) * | 2010-06-14 | 2011-12-22 | Cobalt Technologies, Inc. | Salt selection of microbial mutants to increase bioproduct tolerance, titer, or osmotic shock tolerance |
| CN104630149B (zh) * | 2013-11-08 | 2018-08-21 | 中国科学院广州生物医药与健康研究院 | 外源线粒体导入到哺乳动物细胞中的方法 |
| CN104845924B (zh) * | 2015-04-01 | 2018-03-16 | 农业部沼气科学研究所 | 一株耐呋喃甲醛的运动发酵单胞菌及其制备方法和应用 |
-
2006
- 2006-09-28 WO PCT/US2006/037597 patent/WO2007038564A2/en not_active Ceased
- 2006-09-28 EP EP06825155A patent/EP1943343A2/en not_active Withdrawn
- 2006-09-28 JP JP2008533553A patent/JP2009509533A/ja active Pending
Non-Patent Citations (11)
| Title |
|---|
| JPN6012011297; J. Bacteriol. Vol. 186, No. 12, 2004, p. 4034-4037 * |
| JPN6012011300; J. Mol. Biol. Vol. 203, 1988, p. 29-37 * |
| JPN6012011303; Mol. Gen. Genet. Vol. 184, 1981, p. 166-173 * |
| JPN6012011304; Genetics Vol. 153, 1999, p. 643-652 * |
| JPN6012011305; J. Mol. Biol. Vol. 254, 1995, p. 815-837 * |
| JPN6012011307; J. Bacteriol. Vol. 183, No. 21, 2001, p. 6413-6421 * |
| JPN6013027445; J. Bacteriol. Vol. 187, No. 3, 2005, p. 1022-1035 * |
| JPN6013027447; Biochem. Biophys. Res. Commun. Vol. 288, 2001, p. 385-389 * |
| JPN6013027449; FEMS Microbiol. Lett. Vol. 242, 2005, p. 161-167 * |
| JPN6013027451; J. Biol. Chem. Vol. 279, 2004, p. 54369-54379 * |
| JPN6013027452; J. Bacteriol. Vol. 181, No. 18, 1999, p. 5855-5859 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2016521763A (ja) * | 2013-06-24 | 2016-07-25 | シージェイ チェイルジェダン コーポレーションCj Cheiljedang Corporation | L−スレオニン産生微生物およびこれを用いたl−スレオニンの産生方法 |
| US9758772B2 (en) | 2013-06-24 | 2017-09-12 | Cj Cheiljedang Corporation | L-threonine-producing microorganism and production method for L-threonine using the same |
Also Published As
| Publication number | Publication date |
|---|---|
| EP1943343A2 (en) | 2008-07-16 |
| WO2007038564A2 (en) | 2007-04-05 |
| WO2007038564A3 (en) | 2007-06-28 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US9273307B2 (en) | Global transcription machinery engineering | |
| EP2407541A1 (en) | Global transcription machinery engineering | |
| EP3555278B1 (en) | Thermostable cas9 nucleases | |
| Jin et al. | Improvement of xylose uptake and ethanol production in recombinant Saccharomyces cerevisiae through an inverse metabolic engineering approach | |
| US10711267B2 (en) | Recombinant type I CRISPR-Cas system | |
| Luan et al. | Genome replication engineering assisted continuous evolution (GREACE) to improve microbial tolerance for biofuels production | |
| Woodruff et al. | Engineering improved ethanol production in Escherichia coli with a genome-wide approach | |
| García-Hidalgo et al. | Vanillin production in Pseudomonas: whole-genome sequencing of Pseudomonas sp. strain 9.1 and reannotation of Pseudomonas putida CalA as a vanillin reductase | |
| Maeda et al. | Protein engineering of hydrogenase 3 to enhance hydrogen production | |
| JP2010207094A (ja) | プロトカテク酸の製造法 | |
| JP6746570B2 (ja) | Gal2輸送体のバリアントおよびその使用 | |
| WO2019072596A1 (en) | THERMOSTABLE CAS9 NUCLEASES WITH OFF-TARGET ACTIVITY | |
| Forsberg et al. | Identification of genes conferring tolerance to lignocellulose-derived inhibitors by functional selections in soil metagenomes | |
| JP2009509533A (ja) | 包括的な転写機構エンジニアリング | |
| US20100330614A1 (en) | Global transcription machinery engineering targeting the rnap alpha subunit (rpoa) | |
| US20230119263A1 (en) | Pseudomonas mutant strains with enhanced xylose and galactose utilization | |
| US8193314B2 (en) | Transcriptional engineering of Lactobacillus | |
| HK1126244A (en) | Global transcription machinery engineering | |
| Nyerges et al. | Swapped genetic code blocks viral infections and gene transfer | |
| BRPI0615980A2 (pt) | método de identificação de genes que aumentam a toleráncia ao stress na levedura para melhorar a eficiência da levedura | |
| Vincent et al. | Building a new bacterial orthogonal translation initiation system | |
| JP2025518465A (ja) | キシリトールの生成のための遺伝子改変酵母及び発酵方法 | |
| Si | Genome engineering in Saccharomyces cerevisiae | |
| Hollmann et al. | P-SSM-202 Advanced workflows for the systematic identification of metabolic optimization targets in DBTL-cycles: A demonstrator for producing aromatic compounds in C. glutamicum |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20090928 |
|
| A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20090928 |
|
| A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20120306 |
|
| A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20120604 |
|
| A602 | Written permission of extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A602 Effective date: 20120611 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20120906 |
|
| A02 | Decision of refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A02 Effective date: 20130611 |