JP2009186247A - Analyzing method, analyzing device, and analyzer - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide an analyzing method which enables the calculation of an accurate diluting magnification without being affected by the error of the length of a light path, and an analyzing device loaded with a flow channel constitution capable of realizing this method. <P>SOLUTION: The analyzing method is composed of the first step (the primary photometry in the process 2) for transmitting detection light through a diluted solution in a state that only the diluted solution is held in a mixing chamber (29) to measure the absorbance only of the diluted solution, the second step (the secondary photometry of the process 4) for transmitting the detection light through the diluted liquid sample in a state that only the diluted liquid sample is held in the mixing chamber (29) to measure the absorbance of the diluted liquid sample and the third step (process 9) for correcting the result read by giving access to the reaction matter of the diluted liquid sample in a measuring cell (40a) by the diluting magnification calculated on the basis of the absorbances calculated in the first and second steps to calculate the analyzing result of a component. <P>COPYRIGHT: (C)2009,JPO&INPIT

Description

  The present invention relates generally to the field of multi-item biological fluid analyzers (multi-biosensors) as applied to the analysis of various biological and chemical compositions. More specifically, the present invention performs operations such as liquid metering, transfer, mixing, and agitation using the applied centrifugal force and the capillary force generated by the surface characteristics of the passage in the apparatus and the surface tension of the liquid. The present invention relates to an analysis method for performing analysis, an analysis device, and an analysis apparatus.

  Conventionally, an analytical device that analyzes the characteristics of a sample liquid using the optical scanning technology of the analytical apparatus is put into practical use, using an analytical device that collects the sample liquid inside and rotating the analytical device around the axis center Has been.

  In recent years, there have been many demands from the market, such as sample volume reduction, device miniaturization, short time measurement, multi-item simultaneous measurement, etc., reacting sample liquids such as blood with various analytical reagents, detecting the mixture, There is a demand for a more accurate analyzer that can examine the progress of various diseases in a short time.

  Usually, such an analytical device rarely reacts the sample solution with the reagent as it is, and requires a pretreatment such as dilution of the sample solution with a buffer solution or removal of fine particles in the sample solution depending on the purpose of the analysis. There are many cases. For example, when the sample solution is diluted, it is necessary to accurately derive the dilution factor in the actual measurement value calculation process.

As an example in which such pretreatment and derivation of the dilution rate are optically performed on an analytical device, there is an analytical device disclosed in Patent Document 1.
FIG. 28 shows an analysis device disclosed in Patent Document 1.

  The rotor body 202 has a substantially solid disk shape, and its bottom layer 204 is shown in FIG. A sealed reagent container 206 is located in the chamber 208 of the bottom layer 204, which is radially inward from the outlet channel 210, and the reagent is transferred through the outlet channel 210 into the mixing chamber 212. .

  Reagent container 206 contains a diluent to be mixed with the biological sample. For example, if the sample is blood, standard diluents such as normal saline solution (0.5% saline), phosphate buffer, Ringer's lactic acid solution, and the like may be used. . The sealed reagent container 206 is opened in response to mounting the rotor body 202 into the analyzer. After being opened, the reagent in reagent container 206 flows through outlet channel 210 to mixing chamber 212.

The mixing chamber 212 has a photometrically detectable marker mixture for defining the dilution of the biological sample to be tested.
After mixing, the diluent exits the mixing chamber 212 and enters the metering chamber 216 through the siphon 214. The metering chamber 216 is connected to the overflow chamber 218. The volume of the measuring chamber 216 is smaller than the volume of the reagent container 206. The excess volume of diluent flows into the overflow chamber 218 leaving a predetermined volume of diluent in the metering chamber 216. Excess volume of diluent in overflow chamber 218 enters collection chamber 222 through passage 220.

  The diluent then flows radially outward and into the system cuvette 224 for use as a reference value in the optical analysis of biological samples. A predetermined volume of diluent in the metering chamber 216 enters the separation chamber 228 through the siphon 226, mixes with the biological sample to be analyzed, and dilutes the sample. The sample is added to the rotor body 202 via an inlet in the top layer (not shown).

  The sample weighing chamber 230 is connected to the sample overflow chamber 232 by a connection path 234. The depth of the sample metering chamber 230 and the overflow chamber 232 is selected to be a capillary dimension. The weighed sample then enters separation chamber 228. Separation chamber 228 is used to remove cellular material from a biological sample such as whole blood. The separation chamber 228 includes a cell trap 236 formed on a radially outer circumferential portion and a receiving hole region 238 formed along the radially inner circumference. As a result of centrifugation, there is a capillary region (not shown) between the receiving hole region 238 and the cell trap 236 to prevent it from flowing back after cellular components have entered the cell trap 236. Is formed. The receiving hole region 238 has a volume capable of receiving the diluted cell-free plasma. The diluted plasma enters the second separation chamber 244 from the separation chamber 228 via the siphon 242, where further separation of cellular components is performed.

The diluted sample then exits through passage 246 and into collection chamber 248 where it is sent to cuvette 250 for optical analysis. The cuvette 250 contains reagents necessary for optical analysis of the sample. The dilution factor of the sample can be derived from the optical measurement value of only the diluent obtained by the above method and the optical measurement value of the diluted sample.
JP 7-503794 gazette (FIG. 1)

  In the optical analysis, specifically, in the absorbance measurement using visible light or ultraviolet light employed in the above-described conventional example or the analyzer of the present invention, the optical path length is measured as is obvious from Lambert-Beer's law shown below. It directly affects the results.

A = α ・ L ・ C
α: extinction coefficient, L: material thickness (optical path length), C: sample concentration.

  That is, the optical path length error (manufacturing variation) appears as it is as an error in the measured value. In particular, while the device itself is miniaturized and integrated to reduce the amount of specimen, the optical path length is naturally miniaturized. However, the smaller the optical path length, the greater the effect of the error. However, it is practically impossible to make the system cuvette 224 and the cuvette 250 so that the optical path length error is completely zero. Therefore, there is a problem that an accurate dilution factor cannot be calculated because the influence of each optical path length error is reliably received.

  The present invention solves the above-mentioned conventional problems, and an analysis method capable of calculating an accurate dilution factor without being affected by an optical path length error, and an analysis device equipped with a flow path configuration capable of realizing this analysis method The purpose is to provide.

  In the analysis method according to claim 1 of the present invention, the diluent and the liquid sample are received and mixed in the mixing chamber of the analytical device, and the diluted liquid sample stirred and mixed in the mixing chamber is measured in the measuring device of the analytical device. When the component is analyzed by accessing the reaction product of the diluted liquid sample in the measurement cell, only the diluted liquid is transmitted through the mixing chamber while only the diluted liquid is held in the mixed chamber. A first step of measuring the absorbance of the diluted liquid sample, a second step of measuring the absorbance of the diluted liquid sample by allowing detection light to pass through the mixing chamber while holding the diluted liquid sample in the mixing chamber, The result obtained by accessing and reading the reactant of the diluted liquid sample is corrected by the dilution factor determined based on the absorbance obtained in the first and second steps, and the result of component analysis is calculated. Consisting of a step.

  The analysis method according to a second aspect of the present invention is the analysis method according to the first aspect, wherein the first step measures the absorbance of only the dilution liquid received in the mixing chamber during transfer of the dilution liquid toward the mixing chamber. It is characterized by that.

  The analysis method according to a third aspect of the present invention is the analysis method according to the first aspect, wherein the first step includes a measurement operation for measuring a diluent while the analysis device is rotating, and the analysis device is provided after the measurement operation is completed. And the diluent is transferred to the mixing chamber.

  According to a fourth aspect of the present invention, there is provided an analytical device comprising a diluent chamber for storing a diluent, a liquid sample chamber configured to hold a predetermined amount of a liquid sample, and the liquid sample chamber connected to the liquid sample chamber. A liquid sample holding chamber for temporarily holding the liquid, a diluent quantifying chamber connected to the diluent chamber for quantifying a required amount of the diluent, and connected to the diluent quantifying chamber and transferred to the diluent quantifying chamber. The overflow channel for overflowing the excess of the diluted solution and the liquid sample holding chamber are connected via a first connection channel, and the dilution liquid quantification chamber is connected via a second connection channel. The mixing chamber and the mixing chamber are connected via a capillary channel and provided with a measurement cell for receiving a diluted liquid sample obtained by stirring and mixing the diluent and the liquid sample in the mixing chamber.

The analysis device according to claim 5 of the present invention is characterized in that, in claim 4, the overflow channel is connected to the mixing chamber.
The analysis device according to claim 6 of the present invention is characterized in that, in claim 4, an overflow channel is provided so that the liquid sample exceeding a predetermined amount can be overflowed and measured in the sample solution holding chamber. To do.

  The analytical device according to claim 7 of the present invention is the analytical device according to claim 4, wherein the diluent chamber and the mixing chamber are connected by a distribution channel without going through the diluent quantitation chamber, and the diluent is diluted with the diluent. It is configured so that it can be distributed to the quantification chamber and the mixing chamber.

The analysis device according to claim 8 of the present invention is characterized in that, in claim 4, the first connection channel and the second connection channel have a siphon structure.
The analyzing device according to claim 9 of the present invention is characterized in that, in claim 4, the analyzing device is provided with an overflow chamber communicating with the mixing chamber through a third connection channel having a siphon structure.

  The analysis device according to claim 10 of the present invention is the analysis device according to claim 9, wherein an excessive amount of dilution liquid is added to the mixing chamber further to the inner peripheral side than the innermost peripheral position of the siphon of the third connection channel. It has the 4th overflow channel made to overflow, It is characterized by the above-mentioned.

  According to an eleventh aspect of the present invention, the analytical device according to the fourth aspect is set, and the diluent and the liquid sample are received and mixed in the mixing chamber of the analytical device, and stirred and mixed in the mixing chamber. An analysis apparatus for transferring the diluted liquid sample to the measurement cell of the analytical device and accessing the reactant of the diluted liquid sample in the measurement cell to analyze the component, the analytical device being arranged around the axis Rotation driving means for rotating, a state in which only the diluent is held in the mixing chamber, a state in which the diluted liquid sample in which the diluent and the liquid sample are received and stirred and mixed is held in the mixing chamber, and the measurement cell from the mixing chamber Control means for controlling the rotational drive means to be transferred to the mixing chamber, and the detection light is transmitted through the mixing chamber so that the absorbance of only the dilution liquid, the absorbance of the dilution liquid sample, and the analysis Analyzing means for accessing the reactant based on the sample liquid transferred to the measurement cell of the vice, and the analyzing means reads the result of the analytical means accessing and reading the reactant of the diluted liquid sample in the measuring cell. The component analysis is performed by correcting the absorbance obtained by accessing and reading the mixing chamber holding only the diluent and the absorbance obtained by the analysis means accessing and reading the mixing chamber holding the diluted liquid sample. An operation unit for calculating the result is provided.

  According to the analysis method of the present invention, the dilution rate can be accurately calculated without being affected by the variation in the optical path length of the measurement chamber, and a highly accurate analysis can be performed.

An embodiment of an analytical device of the present invention will be described with reference to FIGS.
FIGS. 1A and 1B show a state in which the protective cap 2 of the analysis device 1 is closed and opened. FIG. 2 shows an exploded view with the lower side in FIG. 1 (a) facing upward, and FIG. 3 shows an assembly drawing thereof.

  As shown in FIG. 1 and FIG. 2, this analytical device 1 includes a base substrate 3 having a microchannel structure having a fine concavo-convex shape on one surface, a cover substrate 4 covering the surface of the base substrate 3, It is composed of four parts including a diluent container 5 holding a diluent and a protective cap 2 for preventing sample liquid scattering.

The base substrate 3 and the cover substrate 4 are joined with the diluent container 5 and the like set therein, and the protective cap 2 is attached to the joined substrate.
By covering the openings of several concave portions formed on the upper surface of the base substrate 3 with the cover substrate 4, a plurality of storage areas described later (same as measurement cells described later) and the microchannels connecting the storage areas A flow path having a structure is formed. Necessary ones in the storage area are preloaded with reagents necessary for various analyses. One side of the protective cap 2 is pivotally supported so that it can be opened and closed by engaging with shafts 6a and 6b formed on the base substrate 3 and the cover substrate 4. When the sample liquid to be examined is blood, the gap between the flow paths of the microchannel structure on which the capillary force acts is set to 50 μm to 300 μm.

  The outline of the analysis process using this analytical device 1 is that the sample solution is spotted on the analytical device 1 in which a diluent is set in advance, and at least a part of the sample solution is diluted with the diluent, and then measurement is performed. It is what.

FIG. 4 shows the shape of the diluent container 5.
4A is a plan view, FIG. 4B is a cross-sectional view taken along line AA of FIG. 4A, FIG. 4C is a side view, FIG. 4D is a rear view, and FIG. These are front views seen from the opening 7. The opening 7 is sealed with an aluminum seal 9 as a seal member after filling the interior 5a of the diluent container 5 with the diluent 8 as shown in FIG. 6 (a). On the opposite side of the diluent container 5 from the opening 7, a latch portion 10 is formed. The dilution liquid container 5 is formed between the base substrate 3 and the cover substrate 4 and is set in the dilution liquid chamber 11 so that the liquid holding position shown in FIG. 6A and the liquid discharge position shown in FIG. Is movably accommodated.

FIG. 5 shows the shape of the protective cap 2.
5A is a plan view, FIG. 5B is a cross-sectional view taken along line BB in FIG. 5A, FIG. 5C is a side view, FIG. 5D is a rear view, and FIG. These are the front views seen from the opening 2a. As shown in FIG. 6 (a) in the closed state shown in FIG. 1 (a), a locking groove 12 with which the latch portion 10 of the diluent container 5 can be engaged is formed inside the protective cap 2. ing.

  FIG. 6A shows the analysis device 1 before use. In this state, the protective cap 2 is closed, and the latch portion 10 of the diluent container 5 is engaged with the locking groove 12 of the protective cap 2 so as to prevent the diluent container 5 from moving in the arrow J direction. Locked in position. In this state, it is supplied to the user.

  When the protective cap 2 is opened as shown in FIG. 1B against the engagement with the latch portion 10 in FIG. 6A when the sample liquid is spotted, the locking groove of the protective cap 2 As shown in FIG. 6 (b), the bottom portion 2b formed with 12 is elastically deformed, and the engagement between the locking groove 12 of the protective cap 2 and the latch portion 10 of the diluent container 5 is released.

  In this state, the sample solution is spotted on the exposed inlet 13 of the analytical device 1 and the protective cap 2 is closed. At this time, by closing the protective cap 2, the wall surface 12 a forming the locking groove 12 comes into contact with the surface 5 b on the side of the protective cap 2 of the latch portion 10 of the diluent container 5, so that the diluent container 5 is pushed in the direction of arrow J (direction approaching the liquid discharge position). In the diluent chamber 11, an opening rib 14 is formed as a protruding portion from the base substrate 3 side. When the diluent container 5 is pushed in by the protective cap 2, an obliquely inclined opening portion of the diluent container 5 is formed. The aluminum seal 9 stretched on the seal surface 7 collides with the opening rib 14 as shown in FIG.

  FIG. 7 shows a manufacturing process in which the analysis device 1 is set in the shipping state shown in FIG. First, before closing the protective cap 2, the groove 42 (see FIGS. 2 and 4 (d)) provided in the lower surface of the diluent container 5 and the hole 43 provided in the cover substrate 4 are aligned, At the liquid holding position, the protrusion 42a of the locking jig 44 provided separately from the base substrate 3 or the cover substrate 4 is engaged with the groove 42 of the diluent container 5 through the hole 43 to hold the diluent container 5 with the liquid. Set in a locked state. Then, the protective cap 2 is closed in a state where the pressing jig 46 is inserted from the notch 45 (see FIG. 1) formed on the upper surface of the protective cap 2 and the bottom surface of the protective cap 2 is pressed and elastically deformed. Can be set to the state shown in FIG. 6A by releasing the pressing jig 46.

  In this embodiment, the case where the groove 42 is provided on the lower surface of the diluent container 5 has been described as an example. However, the groove 42 is provided on the upper surface of the diluent container 5, and the base substrate corresponds to the groove 42. 3 can be configured such that a hole 43 is provided in the groove 3 and the protrusion 44 a of the locking jig 44 is engaged with the groove 42.

  Further, the locking groove 12 of the protective cap 2 directly engages the latch portion 10 of the diluent container 5 to lock the diluent container 5 in the liquid holding position. And the latch portion 10 of the diluent container 5 can be indirectly engaged to lock the diluent container 5 in the liquid holding position.

As shown in FIGS. 8 and 9, the analysis device 1 is set on the rotor 101 of the analyzer 100 with the cover substrate 4 facing downward, so that the component analysis of the sample solution can be performed.
A groove 102 is formed on the upper surface of the rotor 101. When the analysis device 1 is set on the rotor 101, the groove 102 is formed on the rotation support 15 and the protective cap 2 formed on the cover substrate 4 of the analysis device 1. The rotation support portion 16 engages with and accommodates the groove 102.

  When the analysis device 1 is set on the rotor 101 and the door 103 of the analyzer is closed before the rotor 101 is rotated, the set analysis device 1 is moved by the movable piece 104 provided on the door 103 side. The position on the rotational axis of the rotor 101 is pressed against the rotor 101 by the biasing force of the spring 105, and the analysis device 1 rotates integrally with the rotor 101 that is rotationally driven by the rotational driving means 106. Reference numeral 107 denotes an axial center during rotation of the rotor 101. The protective cap 2 is attached in order to prevent the sample liquid adhering to the vicinity of the inlet 13 from being scattered outside due to centrifugal force during analysis.

  As a material of the parts constituting the analysis device 1, a resin material having a low material cost and excellent mass productivity is desirable. Since the analysis apparatus 100 analyzes the sample liquid by an optical measurement method for measuring light transmitted through the analysis device 1, the material of the base substrate 3 and the cover substrate 4 may be PC, PMMA, AS, MS, or the like. A synthetic resin having high transparency is desirable.

  Further, as the material of the diluent container 5, since it is necessary to enclose the diluent 8 in the diluent container 5 for a long period of time, a crystalline synthetic resin having a low moisture permeability such as PP and PE is desirable. As a material for the protective cap 2, there is no particular problem as long as it is a material with good moldability, and an inexpensive resin such as PP or PE is desirable.

  The base substrate 3 and the cover substrate 4 are preferably joined by a method that hardly affects the reaction activity of the reagent carried in the storage area. Ultrasonic welding or laser welding is less likely to generate reactive gas or solvent during joining. Etc. are desirable.

  Further, a hydrophilic treatment for increasing the capillary force is applied to the portion where the solution is transferred by the capillary force due to the minute gap between the substrates 3 and 4 by joining the base substrate 3 and the cover substrate 4. Specifically, hydrophilic treatment using a hydrophilic polymer or a surfactant is performed. Here, hydrophilic means that the contact angle with water is less than 90 °, more preferably less than 40 °.

FIG. 10 shows the configuration of the analyzer 100.
The analysis apparatus 100 includes a rotation driving unit 106 for rotating the rotor 101, an optical measurement unit 108 as an analysis unit that accesses and analyzes the reactant in the analysis device 1, and the rotation speed and rotation of the rotor 101. Control means 109 for controlling the direction and measurement timing of the optical measurement unit 108, a calculation unit 110 for processing a signal obtained by the optical measurement unit 108 and calculating a measurement result, and a result obtained by the calculation unit 110 It is comprised with the display part 111 for displaying.

  The rotation driving means 106 not only rotates the analyzing device 1 around the rotation axis 107 at a predetermined rotation speed around the rotation axis 107 via the rotor 101 but also centers on the rotation axis 107 at a predetermined stop position. The analyzing device 1 can be swung by reciprocating left and right in a predetermined amplitude range and a predetermined cycle.

  The optical measurement unit 108 detects the amount of transmitted light that has passed through the analysis device 1 out of the laser light source 112a that irradiates the measurement cell of the analysis device 1 with laser light and the laser light emitted from the laser light source 112a. The photo detector 113a, the laser light source 112b for irradiating the measurement unit different from the measurement cell of the analysis device 1, and the transmitted light that has passed through the analysis device 1 out of the laser light emitted from the laser light source 112b And a photodetector 113b for detecting the amount of light.

  The analysis device 1 is driven to rotate by the rotor 101, and the sample liquid taken into the inside from the injection port 13 is rotated around the rotation axis 107 located on the inner periphery of the injection port 13. The centrifugal force generated and the capillary force of the capillary channel provided in the analysis device 1 are used to transfer the solution inside the analysis device 1. The channel structure will be described in detail along with the analysis process.

FIG. 11 shows the vicinity of the inlet 13 of the analytical device 1.
FIG. 11A shows an enlarged view of the injection port 13 viewed from the outside of the analytical device 1, and FIG. 11B shows the microchannel structure viewed through the cover substrate 4 from the rotor 101 side. It is.

  The inlet 13 is a gap where a capillary force acts like the guiding part 17 via a guiding part 17 where a capillary force acts in a minute gap δ formed between the base substrate 3 and the cover substrate 4. It is connected to a liquid sample chamber 19 having a volume capable of holding a necessary amount of sample liquid 18. The cross-sectional shape orthogonal to the flow direction of the guide portion 17 (the DD cross section in FIG. 11B) is not a rectangular shape with the back side vertical, but as shown in FIG. It forms in the inclined surface 20 which becomes narrow gradually toward. A bent portion 22 is formed in the guiding portion 17, the liquid sample chamber 19, and the connecting portion to form a recess 21 in the base substrate 3 and change the direction of the passage.

  A sample liquid holding chamber 23 is formed at the tip of the liquid sample chamber 19 as viewed from the guiding portion 17 so that a capillary force is not applied. A cavity 24 having one end connected to the sample solution holding chamber 23 and the other end opened to the atmosphere is formed on a part of the side of the liquid sample chamber 19, the bent portion 22, and the guiding portion 17.

  With this configuration, when blood is spotted on the inlet 13 as the sample liquid 18, the sample liquid 18 is taken into the liquid sample chamber 19 via the guide portion 17. FIG. 12 shows a state before the analysis device 1 after spotting is set on the rotor 101 and rotated. At this time, as explained in FIG. 6C, the aluminum seal 9 of the diluent container 5 collides with the opening rib 14 and is broken. Reference numerals 25a to 25g, 25h, 25i1, 25i2, and 25j to 25n denote air holes formed in the base substrate 3.

FIG. 13 shows the flow path through which the diluent flowing out from the diluent container 5 passes, and the periphery of the mixing chamber 29 for stirring and mixing the diluent or the received diluent and the liquid sample.
The distribution channel is configured as follows so that the diluent flowing out from the diluent chamber 11 can be distributed to the diluent quantification chamber 27 and the mixing chamber 29.

  The dilution liquid quantification chamber 27 disposed on the inner peripheral side of the mixing chamber 29 is connected to the dilution liquid chamber 11 via the discharge channel 26 and quantifies the required amount of the diluted liquid to a surplus amount of the dilution liquid. Overflow. The excess dilution liquid overflowing from the dilution liquid determination chamber 27 is distributed to the mixing chamber 29 via the overflow channel 28. In addition, the outer peripheral side of the dilution liquid quantification chamber 27 is connected to the mixing chamber 29 via a second connection channel 41 having a siphon structure. The outer peripheral side bottom of the mixing chamber 29 communicates with an overflow chamber 36b provided with an inlet on the outer peripheral side of the mixing chamber 29 via a third connection channel 34a having a siphon structure. The overflow chamber 36b is connected to the overflow chambers 36a and 36c via the backflow prevention flow path 35a formed in the gap where the capillary force acts. Further, a fourth overflow channel that causes an excessive amount of dilution liquid in the mixing chamber 29 to overflow into the overflow chamber 36a further to the inner circumferential side than the innermost circumferential position of the siphon of the third connection channel 34a. 34b is provided.

The analysis process will be described together with the configuration of the control means 109 that controls the operation of the rotation drive means 106.
− Step 1 −
The analytical device 1 in which the sample liquid to be inspected is spotted at the inlet 13 holds the sample liquid in the liquid sample chamber 19 as shown in FIG. 14A, and the aluminum seal 9 of the diluent container 5 It is set on the rotor 101 in a torn state.

− Step 2 −
When the rotor 101 is rotationally driven in the clockwise direction (C2 direction) after the door 103 is closed, the held sample solution is broken at the position of the bent portion 22, and the sample solution in the guide portion 17 is discharged into the protective cap 2. Then, the sample liquid 18 in the liquid sample chamber 19 flows into the sample liquid holding chamber 23 as shown in FIG.

The diluent 8 that has flowed out of the diluent container 5 flows into the diluent quantification chamber 27 via the discharge channel 26.
The diluent container 5 has a bottom portion opposite to the opening 7 sealed with the aluminum seal 9 and is formed with an arcuate surface 32 as shown in FIGS. 4 (a) and 4 (b). At the liquid discharge position of the diluent container 5 in the state shown in FIG. 14 (b), the center m of the arc surface 32 is offset by a distance d so as to be closer to the discharge channel 26 side than the rotation axis 107 as shown in FIG. Therefore, the diluent 8 that has flowed toward the arc surface 32 is changed to a flow (in the direction of arrow n) from the outside toward the opening 7 along the arc surface 32, so that the diluent container 5 is efficiently discharged into the diluent chamber 11 from the opening 7.

  When the diluent 8 flowing into the diluent quantification chamber 27 exceeds a predetermined amount, the excess diluent 8 flows into the mixing chamber 29 via the overflow channel 28 as shown in FIG. When the dilution liquid 8 that has flowed into 29 exceeds a predetermined amount, the excess dilution liquid 8 flows into the overflow chambers 36a, 36b, 36c via the third connection channel 34a, the fourth connection channel 34b, and the overflow channel 38. , 36d. The diluent 8 that has flowed into the overflow chambers 36a, 36b, and 36c is held so as not to flow out of the overflow chambers 36a, 36b, and 36c by the capillary force of the backflow prevention flow paths 35a and 35b.

  In the present embodiment, a certain amount of sample liquid is held in the sample liquid holding chamber 23, but when an unmetered sample liquid is supplied to the liquid sample chamber 19 and transferred to the sample liquid holding chamber 23. An overflow channel (not shown) may be provided so that a sample liquid exceeding a predetermined amount overflows from the sample liquid holding chamber and can be measured.

  Here, the diluent 8 is a solution having a specified absorbance in a specific wavelength region, and the absorbance of the diluent 8 is measured while the diluent 8 flowing into the mixing chamber 29 stays in the mixing chamber 29 ( Primary metering). Specifically, the analyzing device 1 is rotationally driven in the clockwise direction (C2 direction), and at the timing when the mixing chamber 29 containing only the diluent 8 passes between the laser light source 112b and the photodetector 113b, the calculation unit 110 is operated. Reads the detection value of the photodetector 113b. P1 in FIG. 14B represents the light transmission position of the primary photometry.

  The third connection channel 34a has a siphon structure having a bent portion in the inner circumferential direction from the outermost peripheral portion of the mixing chamber 29, and the diluent 8 exceeding the bent portion of the third connection channel 34a flows in. Then, the diluent 8 in the mixing chamber 29 is discharged into the overflow chambers 36a, 36b, and 36c by the siphon effect. Further, by providing a connecting channel 34b for discharging a diluent exceeding a predetermined amount at the inner peripheral position of the third connecting channel 34a, the sample solution is mixed from the mixing chamber 29 when an excessive diluent flows. Inflow to the holding chamber 23 is prevented.

  The diluent 8 staying in the mixing chamber 29 is all discharged to the overflow chambers 36a, 36b, 36c with the passage of time, and as shown in FIG. A predetermined amount of the sample solution 18 and the diluted solution 8 are held in the chambers 27, respectively.

− Step 3 −
Next, when the rotation of the rotor 101 is stopped, the sample liquid 18 enters the first connection channel 30 having a siphon shape connecting the sample liquid holding chamber 23 and the mixing chamber 29 as shown in FIG. Similarly, the diluent 8 is also drawn into the second connection channel 41 having a siphon shape that connects the diluent quantification chamber 27 and the mixing chamber 29.

− Step 4 −
When the rotor 101 is rotated counterclockwise (C1 direction), the sample liquid 18 in the sample liquid holding chamber 23 and the diluting liquid 8 in the diluting liquid quantifying chamber 27 flow into the mixing chamber 29 as shown in FIG. At the same time, the diluted plasma component 18a and the blood cell component 18b are centrifuged in the mixing chamber 29. Reference numeral 18c represents a separation interface between the diluted plasma component 18a and the blood cell component 18b. Here, since the sample solution 18 and the diluent 8 once collide with the rib 31 and flow into the mixing chamber 29, the plasma component in the sample solution 18 and the diluent 8 can be uniformly stirred.

  Then, the absorbance of the diluted plasma component 18a centrifuged in the mixing chamber 29 is measured (secondary photometry). Specifically, when the analysis device 1 is rotationally driven in the counterclockwise direction (C1 direction), the calculation unit is at a timing when the mixing chamber 29 containing the diluted plasma component 18a passes between the laser light source 112b and the photodetector 113b. 110 reads the detection value of the photodetector 113b. P2 in FIG. 17A represents the transmission position of the primary photometry light, and the secondary photometry position P2 in the mixing chamber 29 is the same as the primary photometry position P1 shown in FIG. 14B. .

  Even if the primary photometry position P1 and the secondary photometry position P2 are not necessarily the same, both measurements can be expected to improve the measurement accuracy as compared with the prior art by measuring a single mixing chamber 29. Is more desirable.

  Here, in this embodiment, the blood that is the sample liquid 18 and the diluent 8 are directly mixed and then the diluted plasma component 18a is extracted and reacted with a reagent to analyze a specific component in the plasma component. However, since the ratio of the plasma component in the blood varies among individuals, the dilution ratio of the plasma component varies greatly when directly mixed. Therefore, when the diluted plasma component 18a is reacted with the reagent, the reaction concentration varies and affects the measurement accuracy. Therefore, in order to correct the dispersion of the dilution ratio when the sample solution 18 and the diluent 8 are mixed, the absorbance before and after mixing with the sample solution is measured using a diluent having a specified absorbance in a specific wavelength region. Since the dilution rate is calculated by measuring at the same location in the mixing chamber 29, it is possible to eliminate variations in the optical path length of the measurement unit and to receive light due to variations in the surface state (waviness, surface roughness) of the measurement unit. Since the change in the amount can be eliminated, it is possible to measure the dilution ratio with high accuracy and to correct the variation in the dilution ratio with respect to the measurement result in the measurement cell, thereby greatly improving the measurement accuracy. This correction method is also useful for correcting variation in the dilution ratio due to variations in the liquid amounts of the sample liquid 18 and the diluent 8.

− Step 5 −
Next, when the rotation of the rotor 101 is stopped, the diluted plasma component 18a is sucked up into the capillary cavity 33 formed on the wall surface of the mixing chamber 29 and is connected to the capillary cavity 33 through the capillary channel 37 in FIG. As shown in b), it flows into the overflow channel 38, the metering channels 39a, 39b, 39c, 39d, 39e, 39f, and 39g, and the fixed amount is held in the metering channels 39a to 39g.

  FIG. 18A shows a perspective view of the capillary cavity 33 and its periphery. The EE cross section in Fig.18 (a) is shown in FIG.18 (b). The capillary cavity 33 and its periphery will be described in detail.

  The capillary cavity 33 is formed from the bottom 29 b of the mixing chamber 29 toward the inner peripheral side. In other words, the outermost peripheral position of the capillary cavity 33 is formed to extend in the outer peripheral direction from the separation interface 18c between the diluted plasma component 18a and the blood cell component 18b shown in FIG. Thus, by setting the outer peripheral side position of the capillary cavity 33 as described above, the outer peripheral end of the capillary cavity 33 is immersed in the diluted plasma component 18a and the blood cell component 18b separated in the mixing chamber 29. Since the plasma component 18a has a lower viscosity than the blood cell component 18b, the diluted plasma component 18a is preferentially sucked out by the capillary cavity 33, and the capillary channel 37, the overflow channel 38, and the metering channels 39a, 39b. , 39c, 39d, 39e, 39f, and 39g, the diluted plasma component 18a can be transferred toward the measurement cells 40a to 40f and 40g.

  Further, since the blood cell component 18b is also sucked out after the diluted plasma component 18a after the diluted plasma component 18a is sucked out, the path to the middle of the capillary cavity 33 and the capillary channel 37 is replaced with the blood cell component 18b. When the overflow channel 38 and the metering channels 39a to 39g are filled with the diluted plasma component 18a, the transfer of the liquid in the capillary channel 37 and the capillary cavity 33 is stopped. The blood cell component 18b is not mixed into the flow paths 39a to 39g.

Therefore, since the liquid feeding loss can be minimized as compared with the conventional configuration, the amount of the sample liquid necessary for the measurement can be reduced.
-Step 6-
Further, when the rotor 101 is rotationally driven in the counterclockwise direction (C1 direction), as shown in FIG. 19A, the diluted plasma component 18a held in the measurement flow paths 39a to 39g is released into the atmosphere. It breaks at the positions of the bent portions 49a, 49b, 49c, 49d, 49e, 49f, and 49g, which are connected to the cavity 48, and flows into the measurement cells 40a to 40f and 40g. Here, the same amount of diluted plasma component 18a flows into each of the measurement cells 40a to 40f.

  At this time, the diluted plasma component 18a in the overflow channel 38 flows into the overflow chambers 36c and 36a via the overflow chamber 36d and the backflow prevention passage 35b. At this time, the sample liquid in the mixing chamber 29 flows into the overflow chambers 36a and 36c via the siphon-shaped third connection channel 34a and the overflow chamber 36b.

  The shape of the measurement cells 40a to 40f and 40g is a shape extended in the direction in which the centrifugal force acts. Specifically, the width in the circumferential direction of the analysis device 1 from the center of rotation of the analysis device 1 toward the outermost periphery. It is thin. Since the bottoms on the outer peripheral side of the plurality of measurement cells 40a to 40f and 40g are arranged on the same radius of the analyzing device 1, the laser light source 112a having the same wavelength is used to measure the plurality of measurement cells 40a to 40f and 40g. In addition, it is not necessary to arrange a plurality of photodetectors 113a corresponding to them and different radial distances, so that the cost of the apparatus can be reduced and measurement can be performed using a plurality of different wavelengths in the same measurement cell. Measurement sensitivity can be improved by selecting an optimum wavelength according to the concentration.

  Further, on one side wall of the measurement cells 40a, 40b, 40d to 40f in the circumferential direction, capillary areas 47a, 47b, 47d, 47e, and so on extend from the outer peripheral position of the measurement cell to the inner peripheral direction. 47f is formed. The FF cross section in Fig.19 (a) is shown to Fig.21 (a).

  Capillary areas 47c1 and 47c2 are formed on both side walls of the measurement cell 40c in the circumferential direction so as to extend in the inner circumferential direction from the outer circumferential position of the measurement cell. A GG cross section in FIG. 19A is shown in FIG.

Note that the capillary area as seen in the measurement cells 40a to 40f is not formed in the measurement cell 40g.
The capacitable capacity of the capillary area 47a is formed to be smaller than the capacity capable of accommodating all the sample liquid held in the measurement cell 40a. Similarly, the capillary areas 47b and 47d to 47f are formed to have a capacity smaller than the capacity capable of accommodating all the sample liquids held in the respective measurement cells 40b and 40d to 40f. For the capillary areas 47c1 and 47c2 of the measurement cell 40c, the sum of the capacity that can be sucked up in the capillary area 47c1 and the capacity that can be sucked up in the capillary area 47c2 is a capacity that can accommodate all the sample liquid held in the measurement cell 40c. Is formed. The optical path lengths of the measurement cells 40b to 40f and 40g are formed to be the same length.

  As shown in FIG. 20, the capillary areas 47a, 47b, 47c1, 47c2, 47d, 47e, and 47f carry a reagent T1 that reacts with the sample solution. No reagent is provided in the measurement cell 40g.

  In the above-described embodiment, the reagent T1 carried in the capillary areas 47a, 47b, 47c1, 47c2, 47d to 47f differs depending on the specific component to be analyzed, and a reagent that is easily soluble is capillary areas 47a, 47b, 47d to 47f are carried, and the capillary area 47c is made to carry a reagent that is hardly soluble.

-Step 7-
Next, the analysis device 1 is decelerated or stopped, or the analysis device 1 is swung by reciprocating left and right with a predetermined amplitude range and period around the rotation axis 107 at a predetermined stop position. Thus, the sample solution or the mixed solution of the reagent and the sample solution transferred to each of the measurement cells 40a to 40f is sucked up into the capillary areas 47a to 47f by the capillary force as shown in FIG. 19B, and at this time, the reagent T1 And the reaction of the specific component contained in the diluted plasma component 18a and the reagent is started.

− Step 8 −
As shown in FIG. 19B, from the state in which the sample solution or the mixed solution of the reagent and the sample solution is sucked up into the capillary areas 47a to 47f, the rotation of the analysis device 1 is accelerated to make the analysis device 1 When rotationally driven counterclockwise (C1 direction) or clockwise (C2 direction), as shown in FIG. 19A, the liquid held in the capillary areas 47a to 47f is measured by the centrifugal force, thereby measuring cells 40a to 40f. The reagent T1 and the diluted plasma component 18a are agitated by being transferred to the outer peripheral side.

  Here, since the agitation of the reagent and the diluted plasma component 18a is promoted by repeating the operations of the step 7 and the step 8, the agitation can be surely performed in a shorter time than the agitation only by diffusion. It becomes.

− Step 9−
When the analysis device 1 is rotationally driven counterclockwise (C1 direction) or clockwise (C2 direction), each of the measurement cells 40a to 40f and 40g passes through between the laser light source 112a and the photodetector 113a, and the arithmetic unit 110 reads the detection value of the photo detector 113a and corrects it with the result of the primary photometry and the secondary photometry to calculate the density of the specific component.

The measurement result in the measurement cell 40g is used as reference data for the measurement cells 40a to 40f in the calculation process in the calculation unit 110.
In the present embodiment, as shown in FIG. 24, the diluent overflowed from the diluent quantification chamber 27 is transferred to the mixing chamber 29 via the overflow channel 28, and the transferred diluent is transferred to the third connected flow. The absorbance of the diluted solution while being discharged from the mixing chamber 29 to the overflow chamber 36 (overflow chambers 36a, 36b, 36c, 36d, and the backflow prevention passages 35a, 35b) via the channel 34a and the fourth connection channel 34b. However, as shown in FIG. 25, the same effect can be obtained with the configuration excluding the fourth connection channel 34b seen in FIG.

  Further, as shown in FIG. 26, the diluent chamber 11 and the mixing chamber 29 are connected to each other through the connecting channel 301 without passing through the diluent quantification chamber 27, and the diluent transferred from the diluent container 5 is distributed. You may comprise so that it can respectively transfer to the dilution liquid fixed_quantity | quantitative_assay chamber 27 and the mixing chamber 29. FIG. Reference numeral 302 denotes an overflow chamber that stores the diluent that passes through the overflow channel 28.

  Furthermore, as shown in FIG. 27, the position of the siphon bent portion of the first connection channel 30 is configured to be an inner peripheral position relative to the position of the siphon bent portion of the second connection channel 41, and the dilution liquid is After the quantification, the rotation of the analytical device 1 is decelerated and the number of revolutions is controlled so that only the diluent can be transferred to the mixing chamber 29 beyond the bending portion of the siphon. It can be measured by holding it first. Further, in FIG. 27, the siphon bent portion of the first connecting channel 30 is configured to be located at the inner peripheral position than the siphon bent portion of the second connecting channel 41. By arbitrarily setting the relationship between the capillary force and the centrifugal force acting on the respective liquids held in the channel 30 and the second connection flow path 41, the liquid in the second connection flow path 41 first causes the siphon bending portion to move. Since it can be configured to exceed, it is not necessarily limited to the positional relationship between the siphon bent portions of the first connection channel 30 and the second connection channel 41. Parameters for setting the relationship between the capillary force and the centrifugal force include the channel width, the channel depth, the density of the liquid, the height of the liquid level held in the sample solution holding chamber 23 and the diluted solution quantification chamber 27 (liquid Volume, width and depth of each chamber), the radial position of the liquid level, the number of rotations, and the like.

  As described above, since the diluent container 5 can be opened by opening and closing the protective cap 2 when the user collects the sample solution, and the diluent can be transferred into the analysis device 1, the analyzer can be simplified. The cost can be reduced, and the operability for the user can be improved.

  Further, the diluent container 5 sealed with the aluminum seal 9 as the sealing member is used, and the diluent container 5 is opened by breaking the aluminum seal 9 with the opening rib 14 as the protruding portion. The analysis accuracy can be improved without the dilution liquid evaporating and decreasing.

  6A, the latch portion 10 of the diluent container 5 is engaged with the locking groove 12 of the closed protective cap 2, and the diluent container 5 is engaged. Is held at the liquid holding position so that it does not move in the direction of arrow J, so that it can be used even though the diluent container 5 is configured to be movable in the diluent chamber 11 by opening and closing the protective cap 2. Since the position of the diluent container 5 in the diluent chamber 11 is locked at the liquid holding position until the person opens the protective cap 2 for use, the user can use the diluent during transportation before use. There is no case where the container 5 is accidentally opened and the diluted solution is spilled.

  In addition, the width (circumferential dimension) of each of the measurement cells 40a to 40f and 40g formed so as to extend in the centrifugal direction (radial direction) of the analytical device 1 is set to a minimum dimension that can be detected by the optical measurement unit 108. The liquid level height of the liquid held in the measurement cells 40a to 40f and 40g during rotation is defined as a radial position where the optical measurement unit 108 can detect the liquid level height, that is, the liquid level height that fills the laser irradiation area. Therefore, it becomes possible to measure with the minimum necessary liquid amount.

  As described above, the measurement cells 40a to 40f are formed to extend in the direction in which the centrifugal force acts, and extend from the outer peripheral position of the measurement cells 40a to 40f in the inner peripheral direction on at least one side wall of the rotation direction. Since the capillary areas 47a to 47f are formed and the steps 7 to 9 are executed, the capillary tube areas 47a to 47f are configured to include the inflow path 114, the measurement cell 115, and the flow path 117 for stirring the sample solution and the reagent as seen in Patent Document 1. Even if the U-shaped stirring mechanism is not provided, a sufficient stirring effect can be obtained, and the analysis device can be downsized.

  In addition, since the measurement cells 40a to 40f and 40g are formed to extend in the direction in which the centrifugal force acts, the sample liquid for filling the measurement cell is smaller than in the case of Patent Document 1, and a very small amount of sample liquid is required. Can measure.

  In the above embodiment, the reagent T1 is supported on the capillary areas 47a to 47f. However, as shown in FIG. 22, the reagent T1 and a reagent T2 different from the reagent T1 are supported on the capillary areas 47a to 47f. You can also. Further, as shown in FIG. 23, the reagent T1 is provided near the bottom on the outer peripheral side of the measurement cells 40a to 40f, and the capillary areas 47a, 47b, 47c1, 47c2, and 47d to 47f are indicated by virtual lines as necessary. The reagent T2 can also be supported. For a single measurement cell, when the reagent T1 is provided at the bottom of the measurement cell and the reagent T2 is also provided in the capillary area, the reagent T1 and the reagent T2 may be the same component or different from each other. The reagent T2 provided in the capillary area can be a plurality of reagents having different components.

  The analysis method and the analysis device according to the present invention can also be applied to uses such as a medical analysis test apparatus that requires blood analysis and long-term storage of a diluted solution.

FIG. 3 is an external perspective view of the analytical device according to the embodiment of the present invention with the protective cap closed and opened. Exploded perspective view of the analysis device of the same embodiment A perspective view of the analytical device with the protective cap closed as seen from the back Explanatory drawing of the diluent container of the embodiment Explanatory drawing of the protective cap of the embodiment Sectional drawing of the state in which the protective cap is closed before using the analytical device of the same embodiment, when spotting the sample liquid, and after spotting Cross-sectional view of the process to set to the shipment Perspective view just before setting the analysis device to the analyzer Sectional view of the analysis device set in the analyzer Configuration diagram of the analyzer of the same embodiment Expansion explanatory drawing of the principal part of the analysis device of the embodiment Sectional view before setting the analysis device to the analyzer and starting rotation Perspective view of base substrate of analytical device Sectional view after setting the analytical device in the analyzer and rotating and then centrifuging Cross section of the diluent container at the timing when the diluent is released from the rotational axis of the analytical device and the diluent container Sectional view when the solid component of the sample solution after centrifugation is quantitatively collected and diluted Cross section of process 4 and process 5 Capillary cavity 33 and its surrounding enlarged perspective view and EE cross section Sectional drawing of process 6 and process 7 FIG. 12 is an enlarged plan view of the measurement cells 40a to 40f. FF sectional view and GG sectional view in FIG. An enlarged plan view of another example of the measurement cells 40a to 40f An enlarged plan view of still another example of the measurement cells 40a to 40f. Schematic diagram around the mixing chamber 29 in FIG. Schematic of another embodiment around the mixing chamber 29 Schematic of still another embodiment around the mixing chamber 29 Schematic of still another embodiment around the mixing chamber 29 The top view of the principal part of the device for analysis of patent documents 1

Explanation of symbols

DESCRIPTION OF SYMBOLS 1 Analytical device 2 Protective cap 2a Opening 2b Bottom part 3 Base substrate 4 Cover substrate 5 Diluent container 5a Inside 5b Surface of latch part 10 6a, 6b Shaft 7 Opening 8 Diluent 9 Aluminum seal (seal member)
DESCRIPTION OF SYMBOLS 10 Latch part 11 Diluent chamber 12 Locking groove 12a Wall surface 13 Inlet 14 Opening rib (protrusion part)
DESCRIPTION OF SYMBOLS 15,16 Rotation support part 17 Guidance part 18 Sample liquid 18a Diluted plasma component 18b Blood cell component 19 Liquid sample chamber 20 Inclined surface 21 Recessed part 22 Bending part 23 Sample liquid holding chamber 24 Cavity 25a-25h, 25i1, 25i2, 25j-25n Air Hole 26 Discharge flow path 27 Diluent determination chamber 28 Overflow flow path 29 Mixing chamber 30 First connection flow path 31 Rib 32 Arc surface 33 Capillary cavity 34a Third connection flow path 34b Fourth connection flow path 35a, 35b Backflow prevention flow path 36a, 36b, 36c, 36d Overflow chamber 37 Capillary channel 38 Overflow channel 39a, 39b, 39c, 39d, 39e, 39f, 39g Metering channel 40a, 40b, 40c, 40d, 40e, 40f, 40g Measurement cell 41 Second connecting flow path 42 Groove 43 Hole 44 Locking jig 44a Projection 45 Notch 46 Push Jig 47a, 47b, 47c1, 47c2, 47d, 47e, 47f Capillary area 48 Air release cavity 49a, 49b, 49c, 49d, 49e, 49f, 49g Bending part 100 Analyzing device 101 Rotor 102 Groove 103 Door 104 Movable piece 105 Spring 106 Rotation Drive Means 107 Rotation Center 108 Optical Measurement Unit (Analysis Means)
109 Control Unit 110 Calculation Unit 111 Display Unit 112a, 112b Laser Light Source 113a, 113b Photodetector T1, T2 Reagent P1 Primary Photometric Position P2 Secondary Photometric Position

Claims (11)

The dilution liquid and the liquid sample are received and mixed in the mixing chamber of the analytical device, the diluted liquid sample stirred and mixed in the mixing chamber is transferred to the measurement cell of the analytical device, and the diluted liquid sample in the measurement cell When accessing the reactants and analyzing the components,
A first step of measuring the absorbance of only the diluent by allowing detection light to pass through the mixing chamber while holding only the diluent in the mixing chamber;
A second step of measuring the absorbance of the diluted liquid sample by transmitting detection light to the mixed chamber in a state where the diluted liquid sample is held in the mixed chamber;
A result obtained by accessing and reading the reactant of the diluted liquid sample in the measurement cell is corrected by the dilution factor determined based on the absorbance obtained in the first and second steps, and a result of component analysis is calculated. An analysis method comprising steps. The first step includes
The analysis method according to claim 1, wherein the absorbance of only the diluent received in the mixing chamber during transfer of the diluent toward the mixing chamber is measured. The first step includes
2. The analysis method according to claim 1, further comprising a weighing operation of weighing the diluent while the analysis device is rotating, wherein the analysis device is decelerated after the weighing operation and the diluent is transferred to the mixing chamber. . A diluent chamber for storing the diluent,
A liquid sample chamber configured to hold a certain amount of liquid sample;
A liquid sample holding chamber connected to the liquid sample chamber and temporarily holding the liquid sample;
A diluent quantification chamber connected to the diluent chamber for quantifying the required amount of the diluent;
An overflow channel connected to the dilution liquid determination chamber and overflowing an excess of the dilution liquid transferred to the dilution liquid determination chamber;
A mixing chamber connected to the liquid sample holding chamber via a first connection channel and connected to the dilution liquid determination chamber via a second connection channel;
An analytical device provided with a measurement cell that is connected to the mixing chamber via a capillary channel and receives a diluted liquid sample obtained by stirring and mixing the diluent and the liquid sample in the mixing chamber. The analytical device according to claim 4, wherein the overflow channel is connected to the mixing chamber. The analytical device according to claim 4, wherein an overflow channel is provided so that the liquid sample exceeding a predetermined amount can be overflowed and measured in the sample liquid holding chamber. 5. The configuration according to claim 4, wherein the diluent chamber and the mixing chamber are connected by a distribution channel without going through the diluent quantitation chamber, and the diluent can be distributed to the diluent quantitation chamber and the mixing chamber. Analytical device. The analysis device according to claim 4, wherein the first connection channel and the second connection channel have a siphon structure. The analytical device according to claim 4, further comprising an overflow chamber communicating with the mixing chamber via a third connection channel having a siphon structure. 10. The fourth overflow channel for allowing an excessive amount of diluent to overflow further to the inner peripheral side than the innermost peripheral position of the siphon of the third connection channel in the mixing chamber. The analytical device described in 1. The analysis device according to claim 4 is set, and the diluted liquid and the liquid sample are received and mixed in the mixing chamber of the analytical device, and the diluted liquid sample stirred and mixed in the mixing chamber is measured by the analytical device. An analyzer that transfers to the cell and accesses the reactant of the diluted liquid sample in the measurement cell to analyze the components;
Rotational drive means for rotating the analytical device about an axis;
The state where only the diluting liquid is held in the mixing chamber, the state where the diluting liquid sample received in the mixing chamber is stirred and mixed, and the rotation drive so as to be transferred from the mixing chamber to the measuring cell Control means for controlling the means;
Analyzing means for transmitting detection light to the mixing chamber to access the absorbance of only the diluent, the absorbance of the diluted liquid sample, and the reactant based on the sample liquid transferred to the measurement cell of the analytical device;
The analysis means accesses and reads the reaction product of the diluted liquid sample in the measurement cell, and the analysis means accesses the mixing chamber that holds only the diluent and reads the absorbance and the analysis means dilutes. An analyzer provided with an arithmetic unit that calculates the result of component analysis by correcting the dilution factor obtained based on the absorbance read by accessing the mixing chamber holding the liquid sample.

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