CN114839133B - Cell imaging counting device - Google Patents
Cell imaging counting device Download PDFInfo
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- CN114839133B CN114839133B CN202210359739.6A CN202210359739A CN114839133B CN 114839133 B CN114839133 B CN 114839133B CN 202210359739 A CN202210359739 A CN 202210359739A CN 114839133 B CN114839133 B CN 114839133B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Electro-optical investigation, e.g. flow cytometers
- G01N15/1434—Electro-optical investigation, e.g. flow cytometers using an analyser being characterised by its optical arrangement
- G01N15/1436—Electro-optical investigation, e.g. flow cytometers using an analyser being characterised by its optical arrangement the optical arrangement forming an integrated apparatus with the sample container, e.g. a flow cell
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N2015/1006—Investigating individual particles for cytology
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Electro-optical investigation, e.g. flow cytometers
- G01N15/1434—Electro-optical investigation, e.g. flow cytometers using an analyser being characterised by its optical arrangement
- G01N2015/144—Imaging characterised by its optical setup
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Electro-optical investigation, e.g. flow cytometers
- G01N2015/1486—Counting the particles
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
The application provides a cell imaging counting device, including base, apron, install the first base member on the base, install the second base member on the apron and form the micro-fluidic channel between first base member and second base member. Wherein the second matrix is provided with a sample adding hole. The cover plate is movably connected with the base. When the cover plate drives the second matrix to approach the first matrix on the base, the microfluidic channel is formed between the first matrix and the second matrix. The microfluidic channels are in one-to-one correspondence with the sample adding holes, and are communicated with the corresponding sample adding holes. Through such setting, this application need not a large amount of flushing liquid and washs the microfluidic channel, has both environmental protection, has improved work efficiency again, has overcome the coulter cell count method simultaneously and has produced a large amount of sheath liquid waste liquid and the defect that work efficiency is low, has also solved expensive disposable plastics cell count board use cost and white plastics pollution problem in the image method cell count.
Description
Technical Field
The application relates to the technical field of micro-channel devices, in particular to a cell imaging counting device.
Background
Cells have become the conventional research object in basic scientific research, medical clinical and pharmaceutical industries, and cell counting and activity analysis almost become the indispensable works, and how to rapidly and accurately perform cell counting at low cost becomes the primary problem.
Currently, there are two main types of conventional cell counting methods on the market: the image method and the coulter method are operated as follows:
1) Image method: after the cells are dyed, the researchers are added into a special disposable plastic cell counting plate, the disposable plastic cell counting plate is placed into a cell counter for photographing, and the death and the activity of the cells are accurately judged according to the coloring condition of the cells in the photograph, so that the concentration and the total number of the dead cells and the living cells are respectively calculated; the method has high speed and accurate detection result, but after each counting, the disposable plastic cell counting plate for storing the sample is directly discarded, and the disposable plastic counting plate discarded every year in China reaches tens of millions of sheets, so that serious white plastic pollution is caused, and high use cost is caused.
2) Coulter method: researchers process cell samples into single cell suspensions and add the cell suspensions to capillaries or microfluidic chips. The cells generate electric signals one by one through an electric field, and the size of the cells is judged and the number of the cells is counted through the strength of the electric signals. However, after each counting, a great deal of time is spent for cleaning the capillary and the microfluidic chip, and a great deal of waste liquid is generated by the sheath liquid for cleaning, so that the environment is polluted if the treatment is not good; capillary tubes are very prone to clogging if cell suspension preparation is not ideal.
Disclosure of Invention
The present application provides a cell imaging counting device to solve at least some of the problems in the related art.
The cell imaging counting device comprises a base, a cover plate, a first matrix arranged on the base and a second matrix arranged on the cover plate; wherein the second matrix is provided with a sample adding hole; the cell imaging counting device further includes a microfluidic channel formed between the first substrate and the second substrate; wherein:
the cover plate is movably connected with the base; when the cover plate drives the second matrix to approach the first matrix on the base, the microfluidic channel is formed between the first matrix and the second matrix; the microfluidic channels are in one-to-one correspondence with the sample adding holes, and are communicated with the corresponding sample adding holes.
Optionally, a rotating shaft is also arranged; the cover plate is rotatably arranged on the base through the rotating shaft. Through the arrangement, the sample residues on the first substrate and the second substrate can be cleaned or wiped off more conveniently through a rotating connection mode.
Optionally, the base comprises an upper surface, wherein the upper surface is provided with two first protruding blocks; the first protruding block is provided with a mounting hole; the two ends of the rotating shaft are respectively rotatably installed in the mounting holes. By this arrangement, the cover plate can be rotatably mounted above the base.
Optionally, a damper is further included between the cover plate and the base. Through setting up like this, the apron can be under the effect of dead weight more gentle closure to avoid the apron to pound to the base and lead to first base member and/or second base member damage without resistance at all.
Optionally, an installation gap is arranged between the rotating shaft and the base or the cover plate. By this arrangement, the first substrate and the second substrate can remain substantially parallel when the height of the microfluidic channels is adjusted by the regulator, and the heights of the microfluidic channels are ensured to be substantially uniform.
Optionally, a first plane is arranged on the first substrate; the second substrate is provided with second planes which are in one-to-one correspondence with the first planes; when the second substrate approaches the first substrate, the first plane forms the bottom wall of the microfluidic channel, and the second plane forms the top wall of the microfluidic channel. By the arrangement, the formed microfluidic channel is convenient for analyzing, photographing and imaging the sample.
Optionally, a first protruding part is arranged on the upper surface of the first substrate; the first plane is positioned on the upper surface of the first protruding part;
and/or a second bulge part is arranged on the lower surface of the second basal body; the second plane is located on the lower surface of the second boss.
By this arrangement, the sample is restrained by the principle of surface tension by the first plane protruding from the upper surface of the first substrate and/or the second plane protruding from the lower surface of the second substrate, thereby forming individual microfluidic channels.
Optionally, the first substrate includes a recess disposed on an upper surface thereof; the first plane is positioned at the bottom wall of the groove.
A second bulge part is arranged on the lower surface of the second matrix; the second plane is located on a lower surface of the second boss.
By this arrangement, the sample can be restrained by the surface tension by the second plane, thereby forming individual microfluidic channels.
Optionally, the first plane is coplanar with the upper surface of the first substrate;
a second bulge part is arranged on the lower surface of the second matrix; the second plane is located on a lower surface of the second boss.
By this arrangement, the sample can be restrained by the surface tension by the second plane, thereby forming individual microfluidic channels.
Optionally, a first protruding portion is arranged on the upper surface of the first substrate, and the first plane is located on the upper surface of the first protruding portion;
the second plane is coplanar with the lower surface of the second substrate.
By this arrangement, the sample can be restrained by the surface tension through the first plane, thereby forming individual microfluidic channels.
Optionally, the first plane is spaced from the upper surface of the first substrate by no more than 1mm. By this arrangement, when the bottom wall of the microfluidic channel is the bottom wall of the groove on the first substrate, this range can ensure that the groove on the first substrate can be wiped clean relatively conveniently.
Optionally, the edge spacing between two adjacent first planes is not less than 0.01mm; the edge distance between two adjacent second planes is not less than 0.01mm. By the arrangement, as the microfluidic channels are open on four sides, a certain edge gap is arranged between the adjacent microfluidic channels, so that the mixing of samples in different microfluidic channels can be avoided.
Optionally, the first plane is a hydrophobic surface; the second plane is a non-hydrophobic surface or a weak hydrophobic surface. Through the arrangement, a certain hydrophobic property difference is arranged between the first plane and the second plane, so that a sample can be smoothly added into the microfluidic channel from the sample adding hole, and the sample adding is completed.
Meanwhile, the first plane is provided with good hydrophobic performance, and the first plane can be easily wiped clean without residual samples during wiping.
Optionally, a regulator is also arranged on the base; wherein the regulator is used for regulating the height of the microfluidic channel. Through this setting, can adjust the little flow channel height according to the cell sample of equidimension and concentration for this cell counting assembly can adapt to the cell sample of equidimension and concentration, thereby improves the detection effect.
Optionally, the height of the microfluidic channel is no more than 10mm. By the arrangement, the microfluidic channel can be formed, so that the sample can be conveniently analyzed, photographed and imaged.
Optionally, the adjuster is a micron-sized precise thread pair device and is provided with an extension rod; the base is provided with a through hole, the regulator is arranged below the base, and an extending rod of the regulator passes through the through hole and then abuts against the lower surface of the cover plate, so that a gap is formed between the first base body and the second base body. The height range of the micro-flow channel is changed to be a very small range and not more than 10mm, so that the range of the micro-flow channel can be accurately adjusted through the micro-scale precise thread pair device;
meanwhile, the extending rod is propped against the lower surface of the cover plate, so that a gap is formed between the first substrate and the second substrate, and a micro-flow channel with adjustable height is formed between the first substrate and the second substrate.
Optionally, the cell imaging counting device further comprises a third bump and a limiting part; the third protruding block and the limiting part are respectively arranged on the base and the cover plate, and the third protruding block and the limiting part are matched to limit the cover plate to move laterally. The reason for this is that the gap between the cover plate and the rotating shaft allows the cover plate to be provided with a space for lateral movement; therefore, the cover plate is limited to move laterally by the cooperation of the third protruding block and the limiting part; simultaneously, can also make when adjusting the little flow channel height through the regulator, the apron can the vertical lift to guarantee to be in parallel state always between first base member and the second base member.
Optionally, the microfluidic channel is sequentially provided with a sample adding area, a detection area and a sample discharging area along the moving direction of the sample; the outlet end of the sample adding hole is communicated with the sample adding area; the width of the sample discharge area gradually decreases along the moving direction of the sample. By this arrangement, in the process of opening the cover plate upwards, the sample remaining on the second plane can stay in the sample discharge area by the surface tension, thereby avoiding the flow of the remaining sample onto the second substrate.
Optionally, the top wall and the bottom wall of the microfluidic channel are parallel. By such arrangement, the cell imaging effect and detection accuracy in the microfluidic channel can be improved.
Optionally, a second bump is further provided for providing a gap between the first substrate and the second substrate; wherein the second protruding block is positioned on the lower surface of the cover plate or the upper surface of the base. By this arrangement, a gap is provided between the first substrate and the second substrate, thereby forming a highly fixed microfluidic channel.
The application provides a apron in cell imaging counting assembly can drive the second base member and remove. Through setting up like this, when there is the sample to remain in the microfluidic channel, through upwards moving the apron, then clean the sample that remains on first base member and second base member with dust-free cloth, guarantee no remaining pollution-free, consequently solved the remaining problem of current closed microfluidic chip difficult to clean sample.
Furthermore, the cell imaging counting device provided by the application does not need a large amount of flushing liquid to clean the microfluidic channel, thereby protecting the environment, improving the working efficiency, overcoming the defects of large amount of sheath liquid waste liquid and low working efficiency generated by the Coulter cell counting method, and simultaneously solving the problems of the use cost and white plastic pollution of the expensive disposable plastic cell counting plate in the cell counting of the current image method.
It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory only and are not restrictive of the application.
Drawings
The accompanying drawings, which are incorporated in and constitute a part of this specification, illustrate embodiments consistent with the application and together with the description, serve to explain the principles of the application.
FIG. 1 is a schematic view of a cell counting apparatus according to a first embodiment of the invention in an open state;
FIG. 2 is a schematic diagram of a cell counting apparatus according to a first embodiment of the invention in a closed state;
FIG. 3 is a top view of the cell counting device of FIG. 2;
FIG. 4 is a cross-sectional view of A-A of FIG. 3;
FIG. 5 is a partial enlarged view at B in FIG. 4;
FIG. 6 is a top view of a first substrate according to a first embodiment of the invention;
FIG. 7 is a cross-sectional view and enlarged view at C of a first embodiment of a first substrate according to an embodiment of the present invention;
FIG. 8 is a cross-sectional view of a second embodiment of the first substrate in accordance with the first embodiment of the present invention;
FIG. 9 is a cross-sectional view and an enlarged view at D of a third embodiment of a first substrate according to an embodiment of the present invention;
FIG. 10 is a top view of a second substrate according to a first embodiment of the invention;
FIG. 11 is a bottom view of a second substrate according to the first embodiment of the invention;
FIG. 12 is a cross-sectional view and an enlarged view at E of a first embodiment of a second substrate according to example one of the present invention;
fig. 13 is a cross-sectional view of a second embodiment of a second substrate in example one of the present invention.
Detailed Description
Reference will now be made in detail to exemplary embodiments, examples of which are illustrated in the accompanying drawings. When the following description refers to the accompanying drawings, the same numbers in different drawings refer to the same or similar elements, unless otherwise indicated. The implementations described in the following exemplary examples are not representative of all implementations consistent with the present application. Rather, they are merely examples of apparatus and methods consistent with some aspects of the present application as detailed in the accompanying claims.
The terminology used in the present application is for the purpose of describing particular embodiments only and is not intended to be limiting of the present application. Unless defined otherwise, technical or scientific terms used herein should be given the ordinary meaning as understood by one of ordinary skill in the art to which this application belongs. The terms "first," "second," and the like in the description and in the claims, are not used for any order, quantity, or importance, but are used for distinguishing between different elements. Likewise, the terms "a" or "an" and the like do not denote a limitation of quantity, but rather denote the presence of an entity. "plurality" or "plurality" means two or more. Unless otherwise indicated, the terms "front," "rear," "lower," and/or "upper" and the like are merely for convenience of description and are not limited to one location or one spatial orientation. The word "comprising" or "comprises", and the like, means that elements or items appearing before "comprising" or "comprising" are encompassed by the element or item recited after "comprising" or "comprising" and equivalents thereof, and that other elements or items are not excluded. The terms "connected" or "connected," and the like, are not limited to physical or mechanical connections, but may include electrical connections, whether direct or indirect.
The terminology used in the present application is for the purpose of describing particular embodiments only and is not intended to be limiting of the present application. As used in this application and the appended claims, the singular forms "a," "an," and "the" are intended to include the plural forms as well, unless the context clearly indicates otherwise. It should also be understood that the term "and/or" as used herein refers to and encompasses any or all possible combinations of one or more of the associated listed items.
Embodiment one:
as shown in fig. 1 to 4, the present application provides a cell imaging counting device, which comprises a base 1, a cover plate 2, a first substrate 3 mounted on the base 1, and a second substrate 4 mounted on the cover plate 2. The cover plate 2 is rotatably mounted on the base 1. Wherein the first substrate 3 may be mounted on the base 1 by being clamped or adhered, etc., and needs to be maintained in a horizontal state to avoid the sample from flowing out of the microfluidic channel 7. The second base body 4 can be attached to the cover plate 2 by being clamped or glued. The first substrate 3 and the second substrate 4 are made of a transparent material with corrosion resistance, abrasion resistance and high hardness. The first substrate 3 and the second substrate 4 may be selected from quartz or glass.
The cell imaging counting device is shown in the off state in fig. 2. The cell imaging counting device further includes a microfluidic channel (shown in connection with fig. 5) formed between the first substrate and the second substrate. The first and second substrates are substantially parallel. After the sample is added to the microfluidic channel 7 from the sample adding hole 40, the sample can stay in the microfluidic channel 7, and then the sample in the microfluidic channel 7 is photographed and analyzed by a camera, so that the cell counting work is completed. Then the cover plate 2 is turned over to enable the cell imaging counting device to be converted into an open state as shown in fig. 1, and an included angle larger than 90 degrees is formed between the first substrate 3 and the second substrate 4 at the moment, so that the dust-free cloth is convenient to clean residual samples on the first substrate 3 and the second substrate 4, and the problem that the existing closed microfluidic chip is difficult to clean the residual samples is solved. In addition, after cleaning, the cover plate 2 is turned downwards, so that the cell imaging counting device can be reused, and the problems of the use cost of the expensive disposable plastic cell counting plate and white plastic pollution in the cell counting of the current image method are solved. Meanwhile, a large amount of flushing liquid is not needed to clean the microfluidic channel 7, so that the environment is protected, the working efficiency is improved, and the problems of a large amount of sheath liquid waste liquid and low working efficiency generated by a Coulter cell counting method are solved.
In the present embodiment, the cell imaging counting device further comprises a rotation shaft 5. The cover plate 2 is rotatably mounted on the base 1 through the rotating shaft 5. Specifically, the base 1 includes an upper surface, two first protruding blocks 10 are disposed on the upper surface, and mounting holes are disposed on the first protruding blocks 10. The two ends of the rotating shaft 5 are respectively rotatably installed in the installation holes. By this arrangement, the microfluidic channel 7 can be opened or closed more conveniently by turning the cover plate 2.
Of course, in other embodiments, the cover plate 2 can also be mounted on the base 1 in a sliding or lifting manner, so that the microfluidic channel 7 can also be opened or closed.
In the present embodiment, the cell imaging counting device further includes a damper provided between the cover plate 2 and the base 1. By this arrangement, the cover plate 2 can be closed more gently under the action of its own weight, so that damage to the first base body 3 and/or the second base body 4 caused by the cover plate 2 being hit against the base 1 without resistance is avoided.
In the embodiment of the present application, the cell imaging counting device further comprises a regulator 6 mounted on the base 1, wherein the regulator 6 is used for regulating the height of the microfluidic channel 7. Wherein, the regulator 6 can be a micron-sized precise thread pair device provided with an extension rod. The base 1 is provided with a through hole, the regulator 6 is arranged below the base 1, and an extending rod of the regulator 6 passes through the through hole and then abuts against the lower surface of the cover plate 2, so that a gap is formed between the first substrate and the second substrate. This is because the height range of the micro flow channel 7 is changed to a very small range, not more than 10mm, and thus the range of the micro flow channel 7 can be precisely adjusted by the micro-scale precision thread pair device. By such arrangement, if the concentration of the cell sample is high, the height of the microfluidic channel 7 can be adjusted to be low for detection, and if the concentration of the cell sample is low, the height of the microfluidic channel 7 can be adjusted to be high for detection, thereby ensuring good detection effect.
And, the abutment of the protruding rod with the lower surface of the cover plate also enables a gap to be provided between the first substrate 3 and the second substrate 4, thereby forming a height-adjustable microfluidic channel 7 between the first substrate 3 and the second substrate 4.
It should be noted that, a certain installation gap is provided between the cover plate 2, the rotating shaft 5 and the base 1, so that when the height of the micro-flow channels 7 is adjusted by the adjuster 6, the first substrate 3 and the second substrate 4 can still be kept in a substantially parallel state, and the heights of the micro-flow channels 7 are ensured to be substantially consistent.
In the embodiment of the present application, the cell imaging counting device further includes a third bump 8 and a limiting portion. The third bump 8 and the limit portion cooperate to limit lateral movement of the cover plate 2. The limiting part is of a hole structure or a groove structure. The third protruding block 8 is arranged on the cover plate 2, and the limiting part is arranged on the base 1. Of course, in other embodiments, the third protruding block may also be disposed on the base, and the limiting portion may be disposed on the cover plate. This is because the gap between the cover plate 2 and the rotating shaft 5 is such that the cover plate 2 is provided with a space for lateral movement; thus, the cover plate 2 is restricted from moving laterally by the third bump 8 cooperating with the stopper. At the same time, the cover plate 2 can be vertically lifted and lowered when the height of the microfluidic channel is adjusted by the adjuster 6, so that the first substrate 3 and the second substrate 4 are always in a parallel state.
The surface of the first substrate 3 as shown in fig. 6 is an upper surface thereof, on which a plurality of first planes 30 are provided, wherein an edge interval between adjacent two first planes 30 is not less than 0.01mm, thereby avoiding mixing of samples in different first planes 30. In addition, in the embodiment of the present application, only one row of first planes 30 is shown, and in other embodiments, multiple rows of first planes 30 may be provided, so that high throughput cell counting can be achieved, and technical efficiency is greatly improved.
The surface of the second substrate 4 shown in FIG. 10 is an upper surface thereof, on which inlet ends of a plurality of wells 40 are provided.
The surface of the second substrate 4 shown in FIG. 11 is a lower surface thereof on which a plurality of second flat surfaces 41 and outlet ends of a plurality of wells 40 are provided. Wherein, the outlet ends of the plurality of sample adding holes 40 are respectively distributed on the plurality of second planes 41 and are in one-to-one correspondence. Wherein the edge spacing between adjacent second planes 41 is not less than 0.01mm, thereby avoiding sample mixing in different second planes 41. In addition, in the embodiment of the present application, only one row of second planes 41 is shown, and in other embodiments, multiple rows of second planes 41 may be provided, so that high-throughput cell counting can be achieved, and technical efficiency is greatly improved.
In the embodiment of the present application, the first protruding portion 31 is disposed on the upper surface of the first substrate 3. And/or, the lower surface of the second base 4 is provided with a second protruding portion 42. By this arrangement, the sample is restrained by the principle of surface tension by the first plane protruding from the upper surface of the first substrate and/or the second plane protruding from the lower surface of the second substrate, thereby forming individual microfluidic channels 7.
Alternatively, as shown in fig. 7, a plurality of first protrusions 31 are provided on the upper surface of the first base 3. The upper surface of the first protruding portion 31 is the first plane 30. As shown in fig. 13, the second plane 41 is coplanar with the lower surface of the second base 4. The microfluidic channel formed by the cooperation of the first substrate 3 in fig. 7 and the second substrate 4 in fig. 13 can restrain the sample by using surface tension through the first plane, so as to prevent the sample from scattering.
Alternatively, as shown in fig. 8, the first plane 30 is coplanar with the upper surface of the first substrate 3. As shown in fig. 12, a second protrusion 42 is provided on the lower surface of the second base 4. The lower surface of the second protruding portion 42 is the second plane 41. The microfluidic channel formed by the cooperation of the first substrate 3 in fig. 8 and the second substrate 4 in fig. 12 can thus restrain the sample by the surface tension through the second plane, avoiding scattering of the sample.
Alternatively, as shown in fig. 9, a plurality of grooves are provided on the upper surface of the first substrate 3. The bottom wall of the groove is the first plane 30. The first matrix 3 in fig. 9 and the second matrix 4 in fig. 12 thus cooperate to form a microfluidic channel, while the sample is constrained within the microfluidic channel 7 by the groove structure and the surface tension of the second plane.
Of course, the first substrate 3 in fig. 7 and the second substrate 4 in fig. 12 can also cooperate to form a microfluidic channel; and the effect is better.
In the embodiment of the present application, the first plane 30 is a hydrophobic surface. The second plane 41 is a non-hydrophobic or weakly hydrophobic surface. By this arrangement, a certain hydrophobic property difference is provided between the first plane 30 and the second plane 41, so that the sample can be smoothly introduced into the microfluidic channel 7 from the sample introduction hole 40 to complete the sample introduction.
At the same time, the first plane 30 is provided with good hydrophobic properties, and can be easily wiped clean without leaving a sample behind during wiping.
In an embodiment of the present application, the height of the microfluidic channel is not more than 10mm. Through this setting, just can make first plane and second plane cooperation form the microfluidic channel so that conveniently carry out analysis, take a picture and formation of image to the sample.
As seen in fig. 6 and 11, the microfluidic channel 7 is sequentially provided with a sample loading area, a detection area and a sample discharge area along the moving direction of the sample. The loading well 40 is located within the loading zone. The sample, after entering the microfluidic channel 7, mainly stays in the detection zone, the width of the sample discharge zone gradually decreasing in the direction of movement of the sample. By this arrangement, the sample remaining on the second plane 41 during the upward opening of the cover plate 2 can stay in the sample discharge area by the surface tension, thereby avoiding the flow of the remaining sample onto the second substrate 4. In addition, in order to allow the sample to flow into the discharge area during the upward opening of the cover plate 2, the movement direction of the optional sample is perpendicular to the rotation shaft 5.
Embodiment two:
the second embodiment is different from the first embodiment in that: in the second embodiment, no regulator is provided.
In an embodiment of the present application, the cell imaging counting device further comprises a second bump for providing a gap between the first substrate and the second substrate. Wherein the second protruding block is positioned on the lower surface of the cover plate or the upper surface of the base. Such that when the cell imaging counting device is in the off state; the first substrate and the second substrate are prevented from contacting, so that the first plane and the second plane cooperate to form a highly fixed microfluidic channel.
In the event of no conflict, the various embodiments of the present application may complement one another.
Other embodiments of the present application will be apparent to those skilled in the art from consideration of the specification and practice of the application disclosed herein. This application is intended to cover any variations, uses, or adaptations of the application following, in general, the principles of the application and including such departures from the present disclosure as come within known or customary practice within the art to which the application pertains. It is intended that the specification and examples be considered as exemplary only, with a true scope and spirit of the application being indicated by the following claims.
It is to be understood that the present application is not limited to the precise arrangements and instrumentalities shown in the drawings, which have been described above, and that various modifications and changes may be effected without departing from the scope thereof. The scope of the application is limited only by the appended claims.
Claims (13)
1. The cell imaging counting device is characterized by comprising a base (1), a cover plate (2), a first matrix (3) arranged on the base (1) and a second matrix (4) arranged on the cover plate (2); wherein the second substrate (4) is provided with a sample adding hole (40); the cell imaging counting device further comprises a microfluidic channel (7) formed between the first substrate (3) and the second substrate (4); wherein:
the cover plate (2) is movably connected with the base (1); when the cover plate (2) drives the second matrix (4) to approach the first matrix (3) on the base (1), a microfluidic channel (7) is formed between the first matrix (3) and the second matrix (4); the microfluidic channels (7) are in one-to-one correspondence with the sample adding holes (40), and the microfluidic channels (7) are communicated with the corresponding sample adding holes (40);
the first substrate (3) is provided with a plurality of first planes (30); the second substrate (4) is provided with second planes (41) which are in one-to-one correspondence with the first planes (30); when the second substrate (4) approaches the first substrate (3), the first plane (30) forms a bottom wall of the microfluidic channel (7), and the second plane (41) forms a top wall of the microfluidic channel (7);
the upper surface of the first substrate (3) is provided with a plurality of grooves, the bottom wall of each groove is a first plane (30), the lower surface of the second substrate (4) is provided with a second protruding part (42), the lower surface of the second protruding part (42) is a second plane (41), and a microfluidic channel formed by matching the first substrate (3) and the second substrate (4) simultaneously restrains a sample in the microfluidic channel (7) through the groove structure and the surface tension of the second plane; the first plane (30) is a hydrophobic surface; the second plane (41) is a non-hydrophobic or weakly hydrophobic surface;
the cell imaging counting device also comprises a rotating shaft (5); the cover plate (2) is rotatably arranged on the base (1) through the rotating shaft (5), so that samples can flow into a sample discharge area in the process of upwards opening the cover plate (2), and the moving direction of optional samples is perpendicular to the rotating shaft (5).
2. Cell imaging counting device according to claim 1, characterized in that the base (1) comprises two first protruding blocks (10); the first protruding block (10) is positioned on the upper surface of the base (1); the first protruding block (10) is provided with a mounting hole; the two ends of the rotating shaft (5) are respectively rotatably installed in the mounting holes.
3. The cell imaging counting device according to claim 1, further comprising a damper between the base (1) and the cover plate (2).
4. Cell imaging counting device according to claim 1, characterized in that an installation gap is provided between the spindle (5) and the base (1) or cover plate (2).
5. Cell imaging counting device according to claim 1, characterized in that the distance between the first plane (30) and the upper surface of the first substrate (3) is not more than 1mm.
6. The cell imaging counting device according to claim 1, wherein the edge spacing between adjacent first planes (30) is not less than 0.01mm; the edge distance between two adjacent second planes (41) is not less than 0.01mm.
7. The cell imaging counting device according to claim 1, further comprising a regulator (6) mounted on the base (1); wherein the regulator (6) is used for regulating the height of the microfluidic channel (7).
8. Cell imaging counting device according to claim 7, characterized in that the regulator (6) is a micro-scale precision thread pair device provided with an extension rod; the base (1) is provided with a through hole; the regulator (6) is arranged below the base (1); the protruding rod of the regulator (6) is abutted against the lower surface of the cover plate (2) after penetrating through the through hole, so that a gap is formed between the first base body (3) and the second base body (4).
9. The cell imaging counting device according to claim 1, further comprising a third bump (8) and a stop; the third protruding block (8) and the limiting block are respectively arranged on the base (1) and the cover plate (2); the third protruding block (8) and the limiting part are matched to limit the lateral movement of the cover plate (2).
10. Cell imaging counting device according to claim 1, characterized in that the height of the microfluidic channel (7) does not exceed 10mm.
11. The cell imaging counting device according to claim 1, wherein the microfluidic channel (7) is provided with a sample loading area, a detection area and a sample discharge area in sequence along the moving direction of the sample; the outlet end of the sample adding hole is communicated with the sample adding area; the width of the sample discharge area gradually decreases along the moving direction of the sample.
12. Cell imaging counting device according to claim 1, characterized in that the top and bottom walls in the microfluidic channel (7) are distributed in parallel.
13. The cell imaging counting device according to claim 1, further comprising a second bump for providing a gap between the first substrate (3) and the second substrate (4); wherein the second protruding block is positioned on the upper surface of the base (1) or the lower surface of the cover plate (2).
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