JP2009114163A - Hypoglycemic agent and hypoglycemic functional food comprising cultivation product of complex microorganism as active ingredient - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide hypoglycemic agents and foods, having immediate effectivity and excellent hypoglycemic action and derived from natural products. <P>SOLUTION: The hypoglycemic agents and hypoglycemic functional foods comprise cultivation products of complex microorganisms as active ingredients, which products are obtained by a production method comprising a primary complex cultivation step of performing complex cultivation, cultivating microorganism groups comprising a plurality of kinds of microorganisms in one cultivation vessel at the same time, for a plurality of microorganism groups; a fungi collection step of obtaining a plurality of kinds of complex microorganism fungi by separating microorganism fungi in the plurality of kinds of the primary complex cultivation products from each cultivation liquid thereof; a secondary complex cultivation step of cultivating collected the plurality of kinds of complex microorganism fungi in one cultivation vessel at the same time; and a post-treatment step of treating the complex microorganism cultivation products obtained in the secondary complex cultivation by applying heat treatment or the like. <P>COPYRIGHT: (C)2009,JPO&INPIT

Description

Detailed Description of the Invention

  The present invention relates to the production of a hypoglycemic agent containing a cultured product of complex microorganisms as an active ingredient, and a technique for producing a food containing the cultured product of complex microorganisms.

  Conventional hypoglycemic agents are mainly chemical preparations, and examples of using natural products include cases of hypoglycemic agents using plant extracts and microbial cultures described in the following documents: Only.

Reference 1

JP-A-6-56680

Reference 2

JP-A-9-40566

Reference 3

JP-A-10-7777

Reference 4

JP2005-538636

Reference 5

JP 2005-538637 A

Reference 6

JP-A-2005-538630

Reference 7

JP-A-2005-224205

Reference 8

JP 2006-193489 A

Reference 9

JP 2006-273797 A

Reference 10

JP2007-99636

Problems to be solved by the invention

  However, hypoglycemic agents using these natural products have a problem that the period until the hypoglycemic effect appears is long and the hypoglycemic effect is small. There is an urgent need to provide drugs.

Means for solving the problem

  As a result of intensive studies over many years in order to find a natural product-derived hypoglycemic agent that solves the above problems and has an immediate effect and a large hypoglycemic effect, the inventor has found a microorganism group consisting of a plurality of types of microorganisms. A primary complex culture is performed in which a complex culture that is simultaneously cultured in one culture tank is performed for a plurality of types of microorganism groups, and microbial cells in a plurality of types of the primary complex cultures obtained in the primary complex culture are obtained. Then, by separating from each culture solution by filtration or centrifugation, a plurality of types of microbial cells are obtained, and a secondary combined culture is performed in which the plurality of types of microbial cells are simultaneously cultured in one culture tank, and the secondary culture is performed. The secondary complex culture obtained in the complex culture is heat-treated, and the heat-treated secondary complex culture is separated into secondary complex microorganism cells and a second complex culture solution by filtration or centrifugation, The secondary complex microbial cells should have a means for extracting the microbial cell components. Next, an extract of the secondary complex microorganism intracellular component is obtained, and a very small amount of the complex microorganism culture product obtained by mixing the secondary complex microorganism internal component extract and the secondary complex culture solution is obtained. In the case where the blood sugar level is reduced by about 80 mg / dl on average in just one week while ingesting a normal diet, in a case where the blood sugar level is more than 300 in a considerable glutton, In some cases, the blood sugar level decreased to about 170 while continuing to eat a large amount of food, and the cultivated product of the above complex microorganism was convinced of the strong hypoglycemic action and immediate effect, thereby completing the present invention.

The invention's effect

  According to the present invention, it is effective immediately for diabetic patients and exhibits a powerful hypoglycemic action of reducing about 80 mg / dl per week. Is suitable for the prevention and treatment of diabetes, diabetic complications, or lifestyle-related diseases called “metabo”, in which normal blood sugar level is maintained without excessively low blood sugar level and there are no side effects. It provides a means of improving national health that was not available.

  INDUSTRIAL APPLICATION This invention is utilized in the pharmaceutical industry field | area which manufactures the preventive agent and therapeutic agent of diabetes, the medical field | area which prevents and treats diabetes, and the functional food industry field | area which manufactures health food and food for specific health. In addition, it is widely used as a means for differentiating foods in the general food industry.

  It was shown that the culture product of the above complex microorganism can be used as a side effect ameliorating agent for corticosteroids. The culture product of the above complex microorganism not only has a pharmacological action to suppress immune function suppression but also repairs and completely cures the skin. It was presumed that side effects of corticosteroids were prevented and improved by working (Reference 11). However, it has not been confirmed that the culture product of the complex microorganism can be used as a hypoglycemic agent.

Reference 11

JP-A-11-71289
Various microorganisms can be used as the microorganism used in the present invention, and the culture methods are various. For example, the first group: Bacillus bulgaricus A (B. bulgaricus A); Bacillus acidophilus A (B. acidophilus). M. lactisacidi A, group II: Bacillus bulgaricus B (B. bulgaricus B); Bacillus acidophilus B (B. acidophilus); ), Group III: Kornchenbacillus A; Bacillus acidophilus C (B. acidophilus C); Mechnikov lactis acidi C (M. in each group I to IV in Actisacidi C), and Group IV: Kornchenbacillus B; Bacillus acidophilus D (B. acidophilus D); Metnikov lactis acidi D (M. lactisacidi D) Each group of microbial groups that contains at least one type of lactobacilli and lactic acid cocci and a microbial species different from the lactic acid bacteria group is added to or added to a plurality of types of lactic acid bacteria groups having different strains among the groups Each time, primary culture is performed under a predetermined condition, and a preferable result is obtained by combining the obtained cultures of each group and further performing secondary culture. In this case, it is preferable that the metabolite in the primary culture is removed by filtration or the like, and only the cells are further subjected to secondary culture. When the microbial cells obtained by the secondary culture are subjected to heat treatment and other treatments, and metabolites discharged outside the cells during the secondary culture are separated by suction filtration using, for example, a membrane filter, A transparent acidic (lactic acid-containing, PH 3-4) liquid is obtained as the liquid. The culture product of the complex microorganism is obtained by mixing this filtrate with the intracellular component extract of the cells obtained by crushing and extracting the cells as filter cake on the membrane filter.
In applying the complex microorganism culture product as a hypoglycemic agent, a water-soluble therapeutic agent containing 10 microliters of the complex microorganism culture product in 1 ml of a base solution may be taken several times a day. It is not limited to this combination and usage.
Examples of foods containing the above-described complex microorganism culture products include, but are not limited to, cooked rice, bread, udon, ramen, fruit juice, candy, chewing gum, and natto. The mixing ratio of the culture product to these foods may be generally 10 microliters of the culture product per serving of each food, but is not limited to this amount.
Moreover, the culture product of the above complex microorganism may be dripped and eaten on daily food. In this case, a culture product diluted 10-fold with purified water may be dripped into one miso soup (100-200 microliters), for example, but this is not limited to this formulation / use method. Absent.
Furthermore, the physiological activity of the culture product of the above-mentioned complex microorganisms is not limited to lowering blood glucose, but in addition to improving the side effects of the corticosteroid, healing of burns, trauma, wounds, insect bites, wings, healing of athlete's foot, Atopic dermatitis, eczema, swelling, acne, blemishes, buckwheat healing, beautifying skin effect, suppression of skin rash, bad breath, body odor, low back pain, rheumatic pain, joint pain, headache, toothache, neuralgia, stomach cancer pain, allergy Improvement / healing of rhinitis / asthma, improvement / healing of otitis media / periodontal disease, bowel regulation, improvement of digestive system diseases, side effects of anticancer drugs, hair loss prevention / hair growth promotion, hypertension / arteriosclerosis / hyperlipidemia It has been confirmed that there are a wide range of improvements such as improvement of collagen and collagen disease, so applying medicines and foods containing culture products will not only lower blood glucose but also bring about overall health improvement / promotion effects When It is constant.

EXAMPLES The present invention will be described below with reference to examples, but the present invention is not limited to the description.

Group I: Bacillus bulgaricus A (B. bulgaricus A); Bacillus acidophilus A (B. acidophilus); Mechnikov lactis acidi A (M. lactisacidi A), Group II: Bacillus bulgaricus B (B Bulgaricus B); Bacillus acidophilus B (B. acidophilus); Mechnikov Lactisacidi C and Group IV: Kornchenbacillus B (Kornchenbacillus); ); Bacillus acidophilus D (B. acidophilus D); Mechnikov lactis acidi D (M. lactisacidi D) each group is divided into each group, the yeast is different for each lactic acid bacteria group I-IV different in each group Each of the added microbial groups had the following composition: 10 g Trypticase peptone; 5 g Yeast extract; 6 g 1 potassium phosphate; 2 g Ammonium citrate; 20 g Glucose; 1 g Sorbitan monooriate; Magnesium sulfate; 0.120 g Manganese sulfate; 0.034 g Ferrous sulfate; 1 liter Primary complex culture was performed at 37 ° C. for 120 hours using a medium of lactic acid bacteria consisting of purified water (pH 5.5).
The cultures obtained from the microorganisms of groups I to IV were mixed and filtered through a membrane filter, and secondary complex culture was performed at 37 ° C. for 120 hours using the obtained cells as seeds.
The secondary complex cultures obtained from the group I-IV microorganisms to which each of these yeasts has been added are heat-treated at about 65 ° C. for 16 hours, filtered through a membrane filter, and the secondary complex microorganisms and the secondary complex. Separated into a culture solution, the secondary complex microbial cells are ground with a cell grinder, a suspension of the ground material in water is filtered through a membrane filter, and the filtrate is made into cytoplasm inside the cells. An extract of the derived components of the secondary complex microorganism was obtained, and this was mixed with the secondary complex culture solution to obtain a transparent filtrate (culture product of the complex microorganism) having a pH of 3 to 4.
An example of the result of analyzing the culture product of the complex microorganism is shown in Table 1.

Using the cultured product of the complex microorganism produced in Example 1 above, a sample of a hypoglycemic agent was prepared according to the following prescription and subjected to a clinical test.
The hypoglycemic agent sample was placed in a commercially available capsule with 1 milliliter of vinegar, added with 10 microliters of culture product, covered with a capsule, and stored frozen.
With the cooperation of doctors, a clinical study was conducted in which one of the above hypoglycemic agent samples was taken three times a day after breakfast and lunch for 6 diabetic patients who had an average blood glucose level of 200-300. In the study, I continued to take the antidiabetic drugs and dietary instructions that I had been taking until then, and decided to add only the hypoglycemic agent sample, but the blood glucose level dropped to normal and it was normal for over a week. After maintaining the above, it was decided to discontinue taking antidiabetic drugs and dietary guidance, and to continue taking the hypoglycemic agent sample under normal eating habits. The blood glucose level was measured by a doctor, and blood was collected on an empty stomach in the morning (excluding breakfast on the day) for blood glucose level measurement. The course of the above test is shown in FIG.

Using the cultured product of the complex microorganism produced in Example 1 above, a sample of a food product having a hypoglycemic function was prepared according to the following prescription and subjected to a clinical test.
The hypoglycemic food sample is prepared by giving a drop container containing 5 ml of a culture product diluted 10-fold with purified water, and dropping one drop before drinking 180 ml of commercially available liquid yogurt. I drank it.
A clinical trial in which the above-mentioned hypoglycemic function food sample is ingested three times a day after dinner in the morning and afternoon for three diabetic patients and three healthy volunteers who had an average blood glucose level of 200 to 300 with the cooperation of a doctor. The test was conducted. In the study, 3 patients with diabetes continued to take the antidiabetic drugs and dietary guidance they had been taking until then, and only added the hypoglycemic agent sample, but their blood glucose level dropped to normal. However, after maintaining normal values for more than one week, the use of antidiabetic drugs and dietary guidance were discontinued, and only the hypoglycemic agent sample was continued under normal eating habits. Three healthy people take a sample of hypoglycemic function food sample three times a day after dinner in the normal diet and take blood glucose measurements only, and blood is collected on an empty stomach in the morning (excluding breakfast on the day) The blood glucose level was measured. The above test course is shown in FIG.
[Table 1] An example of the analysis result of Example 1 is shown.

The clinical test course of Example 2 is shown. The clinical trial course of Example 3 is shown.

Claims (5)

A primary complex culture is performed in which a complex culture consisting of a plurality of types of microorganisms is simultaneously cultured in a single culture tank for a plurality of types of microorganism groups, and the plurality of types of primary obtained by the primary complex culture are performed. The microbial cells in the composite culture are separated from each culture solution by filtration or centrifugation to obtain a plurality of types of microbial cells, and the plurality of types of microbial cells are simultaneously cultured in one culture tank 2 The secondary complex culture obtained by the secondary complex culture is subjected to heat treatment, and the second complex culture subjected to the heat treatment is subjected to filtration or centrifugal separation with the secondary complex microorganism cells. The secondary complex microbial cells are separated, and the secondary complex microbial cells are subjected to extraction means for microbial cell components to obtain an extract of the secondary complex microbial cell components, and the secondary complex microbial cell components are extracted. And the above-mentioned secondary complex culture solution are mixed and cultured to produce complex microorganisms The resulting, drug and having an effect of lowering blood glucose level in the blood as an active ingredient a culture product of the complex microorganisms. A food comprising the culture product of the complex microorganism according to claim 1 and having an effect of lowering blood glucose concentration in blood. The group of microorganisms according to claim 1 is a group I: B. bulgaricus A; B. acidophilus A; M. lactisacidi A (M. lactisacidi A). ), Group II: Bacillus bulgaricus B (B. bulgaricus B); Bacillus acidophilus B (B. acidophilus); A); Bacillus acidophilus C (B. acidophilus C); Mechnikov lactis acidi C (M. lactisacidi C), and Group IV: Korn In each of the groups I to IV, at least one lactobacilli and lactic acid cocci are included in Enbacillus B (Kornchenbacillus B); B. acidophilus D (M. lactisacidi D); 2. The drug according to claim 1, comprising a group of microorganisms with or without the addition of a microorganism species different from the group of lactic acid bacteria to a plurality of types of lactic acid bacteria groups having different strains among the groups. Or the foodstuff of Claim 2. The heat treatment according to claim 1 is performed under conditions of 40 to 70 ° C for 6 to 24 hours, the drug according to claim 1, the food according to claim 2, and claim 3. The drug or food described in 1. The means for extracting a microbial cell component according to claim 1, the frozen microbial cell is ground with a grinder having an ability to crush the cell wall of the microbial cell, and the ground product is suspended in water. The drug according to claim 1, the food according to claim 2, wherein the filtrate obtained by filtering the suspension with a membrane filter is obtained as an extract derived from the cytoplasm inside the cell body. Item 5. The drug or food according to item 3, and the drug or food according to claim 4.

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