JP2009063324A - Biosensor and manufacturing method therefor - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a biosensor capable of easily performing the attaching work, or the like, to a measuring display device and making it hard to adhere the blood, or the like, which is adhered to the biosensor, to the hand at the time of detaching work, and to provide its manufacturing method. <P>SOLUTION: The manufacturing method of the biosensor 10 includes a process for forming a sensor part 12, by providing a working electrode 18 and a counter electrode 20 on a substrate 16 so as to leave a fixed interval between them and providing a reaction part 22 on the working electrode 18 and the counter electrode 20, before providing a supply port 24 for introducing a specimen into the reaction part 22; a process for forming a support part 14, by providing a working electrode 30 on the side of the support part and a counter electrode 32 on the side of the support part on a support substrate 26, equipped with a bending part 27 so as to leave a definite interval from the vicinity of the bending part 27 to the edge part 26a of the support substrate; and a mounting process for respectively connecting the working electrode 18 and the counter electrode 20 to the electrode 30 on the side of the support part and the electrode 32 on the side of the support part and mounting the sensor part 12 on the support part 14 so as to cross the bending part 27. <P>COPYRIGHT: (C)2009,JPO&INPIT

Description

  The present invention relates to a biosensor that can be quantitatively analyzed by measuring the amount of a substrate component in a specimen, and a method for producing the same.

  Conventionally, biosensors have been devised for measuring blood sugar levels and the like of specimens (see, for example, Patent Document 1 and Patent Document 2). According to this biosensor, the blood glucose level or the like can be measured by measuring the current value between the working electrode and the counter electrode when the reaction part reacts with blood or the like introduced from the supply port.

Japanese Patent Publication No. 08-01208 International Publication No. 2004/017057 Pamphlet

  When measuring a blood glucose level, etc., using a biosensor, the biosensor is inserted into the sensor mounting opening of the measurement display. However, since the biosensor is a consumable item, it is removed from the measurement display after measurement. To be disposed of.

  Here, since the work of removing the biosensor from the measurement display after the measurement was performed while holding the biosensor by a human hand, there is a risk of suffering from an infection from blood adhering to the biosensor. The removal work was done with great care, such as wearing gloves.

  In addition, with biosensors that have been downsized due to recent technological developments, there has been a further increase in the risk of blood adhering to the biosensor adhering to the hand, as well as the attachment and removal of biosensors from measurement displays. Workability and handling are also getting worse. Especially for elderly people and those who are not good at fine fingertips, it is extremely difficult to attach a small biosensor to a measurement display or to remove a biosensor without blood on the hand. .

  Furthermore, as the demand for biosensors increases, a method for manufacturing biosensors that can be efficiently manufactured at lower cost is required.

  In view of the above problems, the inventor of the present application can easily perform attachment and detachment work to and from the measurement display, and blood and the like attached to the biosensor adhere to the hand during the detachment work. As a result of intensive studies to provide a biosensor that is difficult to manufacture and a method for manufacturing the same, the present invention has been achieved.

  That is, the biosensor manufacturing method of the present invention is a biosensor manufacturing method for measuring the amount of a substrate component in a sample, and is provided with a working electrode and a counter electrode on a substrate made of an insulator with a certain interval therebetween, A reaction part is provided on the working electrode and the counter electrode, and then a supply port for introducing a specimen to the reaction part is provided. A sensor part forming step, which is formed on an insulating substrate and provided with a bending part, is bent. A support part-side working electrode and a support part-side counter electrode are provided at regular intervals from the vicinity of the support part to the edge of the support substrate, the support part forming step, and the working electrode and the counter electrode are connected to the support part-side working electrode and the support part. A mounting step of connecting to the part-side counter electrode and mounting the sensor part to the support part so as to intersect the bent part.

  In the biosensor manufacturing method, the support portion forming step includes a step of providing an opening in the support substrate at a position in contact with the bent portion or at a position intersecting with the bent portion. The sensor part is attached to the support part with the supply port protruding into the opening.

  Furthermore, the biosensor of the present invention is manufactured by the method for manufacturing a biosensor.

  According to the biosensor manufactured by the biosensor manufacturing method of the present invention, when the amount of the substrate component in the sample is measured, the supply port of the sensor unit protrudes from the support substrate by bending the support substrate at the bending unit. Therefore, the support substrate does not hinder measurement work, and it is possible to easily spot blood or the like on the supply port. On the other hand, after the measurement, since the sensor unit including the supply port is accommodated in the outer peripheral edge of the support substrate, when the biosensor is removed from the measurement display, it is possible to prevent adhesion of blood or the like adhering to the biosensor to the hand. . Therefore, it is possible to prevent infection from the blood adhering to the sensor unit.

  In addition, the biosensor manufactured according to the present invention can easily adhere to blood and the like, prevent infections, etc. due to its structure. The improvement of property etc. is also achieved.

  Furthermore, since the biosensor manufactured according to the present invention is configured by mounting a small sensor unit on the support unit, the biosensor can be easily attached to and detached from the measurement indicator, in particular. For an elderly person or a person who is not good at fine work with a fingertip, etc., handling property, workability of measurement work, etc. can be improved.

  Furthermore, in the biosensor manufacturing method of the present invention, the sensor unit and the support unit are manufactured separately, and then the sensor unit is mounted at a predetermined position in the support unit. Can be manufactured efficiently.

  Hereinafter, embodiments of a biosensor of the present invention and a method of manufacturing the biosensor will be described with reference to the drawings. A biosensor 10 according to the present embodiment shown in FIGS. 1A and 1B is a biosensor that measures the amount of a substrate component in a specimen, and includes a sensor unit 12 and a support unit 14. Has been.

  The sensor unit 12 is provided on a substrate 16 made of an insulator, a working electrode 18 provided on the substrate 16, a counter electrode 20 provided at a fixed interval from the working electrode 18, the working electrode 18 and the counter electrode 20. And a supply port 24 for introducing a sample such as blood or urine to the reaction unit 22, and the reaction unit 22 reacts with the sample introduced from the supply port 24, thereby The amount of the substrate component (for example, blood glucose level) can be measured.

  The support portion 14 is made of an insulator, and has a support substrate 26 provided with a bent portion 27. The support portion side action extends on the support substrate 26 from the vicinity of the bent portion 27 to the edge portion of the support substrate 26 (= support substrate edge portion 26a). On the electrode 30 and also on the support substrate 26, there is provided a support portion side counter electrode 32 that extends from the vicinity of the bent portion 27 to the support substrate edge portion 26 a with a certain distance from the support portion side working electrode 30. The bent portion 27 is a place where the support substrate 26 is bent. In the present embodiment, the support substrate 26 is bent at the bent portion 27 as shown in FIG. The effect of the bent portion 27 will be described later. In the present application, the “near the bent portion” includes the position of the bent portion.

  The biosensor 10 of the present embodiment is manufactured by mounting the sensor unit 12 obtained through the sensor unit forming process on the support unit 14 obtained through the support unit forming process in the mounting process.

  In the formation process of the sensor portion, as shown in FIG. 3, first, the working electrode 18 and the counter electrode 20 having a band shape are provided on the substrate 16 in parallel at a constant interval (FIG. 3A). After providing the reaction part 22 on 18 and the counter electrode 20, the sensor part 12 is obtained by providing the supply port 24 which introduce | transduces a test substance into the reaction part 22 (FIG. 2, FIG.3 (b)). The supply port 24 is formed by the leading edge 36 a of the cover 36 mounted on the substrate 16 via the spacer 34 and the leading edge 16 a of the substrate 16. In other words, the supply port 24 is formed by the gap formed between the leading edge 36 a and the leading edge 16 a by the spacer 34. A sample such as blood introduced from the supply port 24 can reach the leading edge 34a of the spacer 34 by capillary action if the amount is sufficient.

  In the supporting portion forming step, the supporting portion side working electrode is formed in a strip shape in parallel with a certain distance from the vicinity of the bending portion 27 to the supporting substrate edge portion 26a on the supporting substrate 26 including the bent portion 27 and made of an insulator. By providing 30 and the support portion side counter electrode 32, the support portion 14 is obtained. In the present embodiment, a cutout 27 a for facilitating the bending of the support substrate 26 is also formed in the bent portion 27.

  Here, the insulator which comprises the board | substrate 16 in the sensor part 12, and the support substrate 26 in the support part 14 is biodegradable polyester which consists of a polyethylene terephthalate (PET), a polyethylene naphthalate, an aliphatic unit, and an aromatic unit, for example. Examples thereof include polyester-based resin sheets such as resins, polyamide-imide sheets that are more excellent in heat resistance, chemical resistance, and strength, plastic sheets such as polyimide sheets, and inorganic substrates such as ceramics.

  The working electrode 18, the counter electrode 20, the support part side working electrode 30, and the support part side counter electrode 32 are formed on the substrate 16 or the support substrate 26, for example, platinum, gold, nickel, palladium, indium-tin oxide, or the like. It is formed of a good conductor. As a method of forming the working electrode 18 and the like, hot stamping can be considered. Vacuum deposition or sputtering is preferable because a fine electrode pattern can be formed with high accuracy. In the case of sputtering, it can be formed at once by masking the outside of electrode formation.

  Further, the reaction unit 22 includes an oxidoreductase and an electron acceptor. For example, it is configured by applying and drying a liquid material containing an oxidoreductase and an electron acceptor. Examples of the oxidoreductase include glucose oxidase when measuring glucose. Glucose oxidase reacts with glucose to produce gluconic acid and hydrogen peroxide. Moreover, when measuring an alcohol value, alcohol oxidase or alcohol dehydrogenase is used. When measuring lactic acid, lactate oxidase or lactate dehydrogenase is used. When measuring uric acid, uricase is used. Generally, it is an inorganic or organic fine powdery compound that promotes oxidation-reduction of enzymes. For example, alkali metal ferricyanide (particularly potassium ferricyanide is preferred), ferrocene or an alkyl-substituted product thereof, p-benzoquinone, methylene blue, potassium β-naphthoquinone-4-sulfonate, phenazine methosulfate, 2,6- Examples include dichlorophenol-indophenol. Ferricyanide alkali metal salts and ferrocenes have a stable function as an electron transfer medium and are well soluble in aqueous solvents such as water, alcohols, or mixed solvents thereof, and thus effectively function as electron acceptors.

  And the biosensor 10 of this embodiment is obtained by mounting | wearing the support part 14 obtained through the formation process of a support part with the sensor part 12 obtained through the formation process of a support part in the attachment process. . In this mounting process, the working electrode 18 and the counter electrode 20 provided in the sensor unit 12 are respectively connected to the support unit side working electrode 30 and the support unit side counter electrode 32 provided in the support unit 14, and the sensor unit. 12 is attached to the support portion 14 in such a state that it intersects the bent portion 27. That is, the sensor unit 12 is configured such that the supply port 24 provided in the sensor unit 12 protrudes from the bent portion 27 in the direction opposite to the support substrate edge 26a (= tip portion 29) on the support substrate 26. Mounted on the support 14.

  The biosensor 10 obtained through the sensor part forming process, the supporting part forming process, and the mounting process described above has a sensor mounting opening provided in a measurement display (not shown) when measuring the amount of a substrate component such as a blood glucose level. Inserted into the section. Below, the effect | action at the time of measuring a blood glucose level using this biosensor 10 is demonstrated.

  The blood glucose level measurer first inserts the support substrate edge portion 26a side of the support portion 14 of the biosensor 10 into a sensor mounting opening of a measurement display (not shown). Next, the support substrate 26 is bent by the bending portion 27 so that the supply port 24 in the sensor portion 12 protrudes from the support substrate 26 as shown in FIG. Specifically, the front end portion 29 side of the support substrate 26 is bent by the bending portion 27 toward the support substrate edge portion 26a. Since the supply port 24 protrudes from the bent portion 27 toward the distal end portion 29 of the support substrate 26, when the support substrate 26 is bent by the bent portion 27, the supply port 24 protrudes from the bent portion 27 toward the distal end portion 29 side of the support substrate 26. The supply port 24 that has been in a state of protruding from the outer peripheral edge of the support substrate 26.

  In the state where the supply port 24 of the sensor unit 12 is protruded from the support substrate 26 as described above, blood that has been pierced by a fingertip or the like is spotted on the supply port 24. The blood spotted on the supply port 24 flows through the gap between the substrate 16 and the cover 36 along the working electrode 18 and the counter electrode 20 toward the tip edge 34a of the spacer 34 by capillary action. Then, an enzymatic reaction is performed in the reaction unit 22, and the blood glucose level is measured by detecting the electrochemical change at that time with the working electrode 18 and the counter electrode 20, and the measurement result is displayed on the measurement display.

  After the blood glucose level is measured, the front end 29 side of the support substrate 26 that has been bent by the bent portion 27 is returned to the original state. And a series of blood glucose level measurement work is complete | finished by hold | gripping the front-end | tip part 29 side of the support substrate 26 returned to the origin, and removing the biosensor 10 from the sensor attachment opening part of a measurement display.

  As described above, according to the biosensor 10 of the present embodiment, when the amount of a substrate component such as a blood glucose level is measured, the supply port 24 protrudes from the support substrate 26 by bending the support substrate 26 with the bending portion 27. Therefore, the support substrate 26 does not interfere with the measurement work, and the spotting of blood or the like to the supply port 24 can be easily performed as in the conventional biosensor. On the other hand, after the measurement, since the sensor unit 12 including the supply port 24 is accommodated in the outer peripheral edge portion of the support substrate 26, when the biosensor 10 is removed from the measurement display, the blood or the like attached to the vicinity of the supply port 24 Can be prevented. Accordingly, it is possible to prevent infection from the blood adhering to the biosensor 10.

  The cover 36 in the sensor unit 12 is transparent so that blood flowing from the supply port 24 toward the tip edge 34a of the spacer 34 can be visually recognized. However, the used biosensor 10 is discarded. At this time, since blood or the like attached to the sensor unit 12 is covered with the support substrate 26 and cannot be visually recognized, discomfort at the time of disposal can be eliminated.

  Furthermore, because of the structure of the biosensor 10, it is easier to attach blood or the like to the hand, prevent infections, etc., compared to conventional biosensors. The improvement of handling is also possible.

  In addition, since the biosensor 10 of the present embodiment is configured by mounting the small sensor unit 12 on the support unit 14, the biosensor 10 can be easily attached to and detached from the measurement display. Especially for elderly people and people who are not good at fine work with fingertips, the handling property and workability of measurement work can be improved as compared with conventional biosensors.

  Furthermore, in the manufacturing method of the biosensor 10 according to the present embodiment, the sensor unit 12 and the support unit 14 are manufactured separately, and then the sensor unit 12 is mounted at a predetermined position in the support unit 14. Can be efficiently manufactured at low cost.

  The biosensor 10 and the manufacturing method thereof according to the embodiment of the present invention exemplified above should not be construed as substantially limiting the technical idea of the present invention. For example, the position of the bent portion 27 on the support substrate 26 is not limited to the position shown in FIGS. As above-mentioned, the bending part in this invention means the location where a support substrate is bent at the time of the measurement of a substrate component amount. Therefore, as long as it is provided in a direction crossing the sensor unit 12 as in the bent portion 31 shown in FIG. 5A, it may be formed in an oblique direction with respect to the longitudinal direction of the support unit 14. Even when the support substrate 26 in the support portion 14 is bent by the bent portion 31, the sensor portion 12 is attached to the support portion 14 so as to intersect the bent portion 31, so that the supply port 24 of the sensor portion 12 can be The support substrate 26 protrudes from the outer peripheral edge portion, and when the amount of the substrate component is measured, the support substrate 26 does not prevent spotting of blood or the like to the supply port 24.

  Further, in the above embodiment, the notch 27a for enabling easy folding is formed in the bent portion 27 in advance, but the biosensor of the present invention is the biosensor shown in FIGS. As in 10a and 10b, an embodiment may be provided that includes a support portion that is easily bent at a predetermined position without providing a notch in the bent portion. In the support portion 14a provided in the biosensor 10a shown in FIG. 5B, the side edges 43 and 43 'of the support substrate 40 are provided with recesses 44 and 44', respectively. Therefore, when the support substrate 40 is to be bent, the bent portion 41 connecting the recesses 44 and 44 ′ provided in the side edge portions 43 and 43 ′ of the support substrate 40 is induced, and as a result, the bending is performed. The supply port 24 of the sensor unit 12 mounted on the support unit 14 a so as to intersect the unit 41 can be protruded from the outer peripheral edge of the support substrate 40.

  The support portion 14b provided in the biosensor 10b shown in FIG. 5C has a bulge 48 formed on the tip 49 side of the support substrate 46, and a boundary line between the bulge 48 and the other portion of the support substrate 46. 47, the sensor part 12 is mounted on the support part 14b in a state where the supply port 24 protrudes toward the expansion part 48 side. Accordingly, when the support substrate 46 is to be bent, the bent portion 45 is induced at the boundary line 47, and as a result, the sensor portion mounted on the support portion 14 b so as to intersect the bent portion 45. The twelve supply ports 24 can protrude from the outer peripheral edge of the support substrate 46.

  Further, like the support portion 14c in the biosensor 10c shown in FIG. 6, the support substrate 50 is L-shaped bent in the vertical direction in the middle of its longitudinal direction, or bent or curved in the oblique lateral direction, etc. It may be.

  The biosensors 10, 10a to 10c according to the embodiments of the present invention exemplified above should not be construed as substantially limiting the technical idea of the present invention. A biosensor 10d according to another embodiment of the present invention shown in FIGS. 7A and 7B is a biosensor that measures the amount of a substrate component in a specimen, and is similar to the biosensor 10 described above. 12 and a support portion 14d. In addition, below, description is abbreviate | omitted about the member etc. which were attached | subjected the code | symbol same as the above-mentioned code | symbol.

  In the step of forming the support portion 14 d in the biosensor 10 d of the present embodiment, the opening 25 is provided in the support substrate 28 at a position in contact with the bent portion 27. Then, the bent portion 27, that is, the side of the support portion that forms a belt shape in parallel with a certain distance from the edge portion of the opening 25 (= opening edge portion 25a) to the edge portion of the support substrate 28 (= support substrate edge portion 28a). A working electrode 30 and a support portion side counter electrode 32 are provided.

  The sensor portion 12 is attached to the support portion 14d obtained through the support portion forming step in the subsequent attachment step. Specifically, the working electrode 18 and the counter electrode 20 provided in the sensor unit 12 are respectively connected to the support unit side working electrode 30 and the support unit side counter electrode 32 provided in the support unit 14d, and the sensor unit. In this embodiment, the sensor unit 12 is mounted on the support unit 14d in a state in which the sensor 12 crosses the bent portion 27, in other words, the supply port 24 provided in the sensor unit 12 protrudes into the opening 25. The biosensor 10d is obtained.

  The biosensor 10d of this embodiment is inserted into a sensor mounting opening provided in a measurement display (not shown) when measuring the amount of a substrate component such as a blood glucose level. Below, the effect | action at the time of measuring a blood glucose level using this biosensor 10d is demonstrated.

  A blood glucose level measurer first inserts the support substrate edge portion 28a side of the support portion 14d of the biosensor 10d into a sensor mounting opening of a measurement display (not shown). Next, the support substrate 28 is bent at a bent portion 27 along the opening edge portion 25a, and the supply port 24 in the sensor unit 12 protrudes from the support substrate 28 as shown in FIG. Specifically, the front end 29 side, which is the edge opposite to the support substrate edge 28 a of the support substrate 28, is bent to the support substrate edge 28 a side by the bending portion 27. The supply port 24 protrudes into the opening 25, and the bent portion 27 (opening edge portion 25 a) is located on the support substrate edge portion 28 a side from the supply port 24, so that the support substrate 28 is bent by the bent portion 27 to bend. The supply port 24 protruding from the portion 27 into the opening 25 provided on the distal end portion 29 side of the support substrate 28 is in a state of protruding from the outer peripheral edge portion of the support substrate 28. Note that the bending direction of the support substrate 28 is not particularly limited, and the supporting substrate 28 may be bent on the side opposite to the illustrated direction, that is, on the side where the sensor unit 12 is mounted.

  After the supply port 24 of the sensor unit 12 protrudes from the support substrate 28 as described above, the blood glucose level is measured in the same manner as the biosensor 10 of the above embodiment. After the blood glucose level is measured, the front end portion 29 side of the support substrate 28 that has been bent by the bent portion 27 is returned to the original state. And a series of blood glucose level measurement work is complete | finished by hold | gripping the front-end | tip part 29 side of the support substrate 28 returned to the original, and removing the biosensor 10d from the sensor attachment opening part of a measurement display.

  As described above, also with the biosensor 10d of the present embodiment, the supply port 24 protrudes from the support substrate 28 when measuring the amount of the substrate component such as blood glucose level, so that the support substrate 28 hinders the measurement work. In the same manner as in the conventional biosensor, it is possible to easily spot blood or the like on the supply port 24. On the other hand, after the measurement, the sensor unit 12 including the supply port 24 is accommodated in the outer peripheral edge portion of the support substrate 28. Therefore, when the biosensor 10d is removed from the measurement display, it is applied to the hand such as blood attached to the sensor unit 12. Adhesion can be prevented. Therefore, it is possible to prevent infection from blood adhering to the sensor unit 12.

  Also in the manufacturing method of the biosensor 10d of the present embodiment, after the sensor unit 12 and the support unit 14d are manufactured separately, the sensor unit 12 is mounted at a predetermined position in the support unit 14d. The obtained biosensor 10d can be efficiently manufactured at low cost.

  The biosensor 10d according to the present embodiment exemplified above should not be construed as substantially limiting the technical idea of the present invention. For example, in the step of forming the support portion, the position where the opening 25 is provided is not limited to the position in contact with the bent portion 27 as shown in FIGS. 7 and 8 and may be provided at a position that intersects the bent portion. . In other words, the position of the bent portion provided in the support substrate 28 may be a position that coincides with the opening edge portion 25a of the opening 25 provided in the support substrate 28 as in the biosensor 10d illustrated in FIG. Further, it may be located at a location intersecting with the opening 25 as in the bent portions 33a and 33b shown in FIG. The opening 25 is provided at a position intersecting with the bent portions 33a and 33b. In the mounting process, the supply port 24 is protruded from the bent portions 33a and 33b toward the front end portion 29 of the support substrate 28 in the mounting process. When the sensor unit 12 is attached to the support part 14d in a state where the support substrate 28 is bent, the supply port 24 protrudes from the outer peripheral edge of the support substrate 28 when the support substrate 28 is bent by the bent part 33a or the like. When measuring the amount of components, spotting of blood or the like to the supply port 24 is not hindered by the support substrate 28.

  Moreover, the aspect provided with the support part made easy to bend in a predetermined position like the biosensors 10e and 10f shown to FIG.9 (b) (c) may be sufficient. In the support part 14e provided in the biosensor 10e shown in FIG. 9B, the side edges 43 and 43 'of the support substrate 60 are provided with recesses 44 and 44', respectively. An opening 25 is provided at a position intersecting the line connecting the recesses 44 and 44 ′, and the sensor portion 12 is mounted on the support portion 14 e with the supply port 24 of the sensor portion 12 protruding into the opening 25. Has been. Therefore, when the support substrate 60 is to be bent, the bent portions 61 that connect the recesses 44 and 44 ′ provided in the side edge portions 43 and 43 ′ of the support substrate 60 are induced, and as a result, the openings The supply port 24 that protrudes into the 25 can be protruded from the outer peripheral edge of the support substrate 60.

  The support portion 14f provided in the biosensor 10f shown in FIG. 9C has a bulge 48 formed on the tip 69 side of the support substrate 62, and a boundary line between the bulge 48 and the other portion of the support substrate 62. An opening 25 is provided at a position intersecting 63. And the sensor part 12 is mounted | worn with the support part 14f in the state which made the supply port 24 protrude in this opening 25. FIG. Therefore, when the support substrate 62 is to be bent, the bent portion 64 is induced at the boundary line 63, and as a result, the supply port 24 protruding into the opening 25 is connected to the outer peripheral edge portion of the support substrate 62. Can be protruded from.

  In the embodiment shown in FIG. 6, an opening having the bent portion 27 as an opening edge may be provided, or an opening may be provided at a position intersecting the bent portion 27. That is, the front view shape of the support portion in the present invention is not limited to a square shape such as the biosensors 10, 10a, 10d, and 10e, but is a square shape and a circular shape like the support substrates 46 and 62 in the biosensors 10b and 10f. The shape may be any of a shape combining a square, an ellipse, a sector, a shape combining other polygons, and the like. Furthermore, like the support part 14c in the biosensor 10c shown in FIG. 6, the support substrate may be bent or curved in the longitudinal direction.

  In addition, the shape of the working electrode and the counter electrode in the sensor unit, the number of electrodes provided on the sensor unit and the support unit, and the like are not particularly limited, and the mounting method of the sensor unit to the support unit is particularly limited, such as welding, adhesion, and adhesion. Not.

  Furthermore, in the method for producing a biosensor of the present invention, the sensor part and the support part may be formed by providing individual electrodes on the continuous substrate and the support substrate and then dividing each of them in each forming step. Alternatively, the sensor part and the support part may be formed individually. Also in the mounting process, after mounting a series of sensor units and support units, they may be divided into individual biosensors, or the individual sensor units and support units may be mounted individually. The present invention can be carried out without departing from the gist thereof, with appropriate improvements, changes or additions based on the inventive ideas and ideas of those skilled in the art.

(A) is a front view of the biosensor which concerns on one Embodiment of this invention, (b) is A-A 'sectional drawing in (a). It is an enlarged front view of the sensor part in FIG. It is explanatory drawing of the assembly method of the sensor part shown in FIG. 2, (a) is a front view before providing the reaction part, (b) is a front view which shows the state which provides a spacer and a cover. It is sectional drawing which shows the state which bent the biosensor shown in FIG. 1 by the bending part. (A)-(c) is a front view which shows the biosensor which concerns on other embodiment of this invention. It is a front view which shows the biosensor which concerns on other embodiment of this invention. (A) is a front view of the biosensor which concerns on other embodiment of this invention, (b) is B-B 'sectional drawing in (a). It is sectional drawing which shows the state which bent the biosensor shown in FIG. 7 by the bending part. (A)-(c) is a front view which shows the biosensor which concerns on other embodiment of this invention.

Explanation of symbols

10, 10a to 10f: Biosensor 12: Sensor unit 14, 14a to 14f: Support unit 16: Substrate 18: Working electrode 20: Counter electrode 22: Reaction unit 24: Supply port 25: Opening 25a: Opening edge 26, 28 , 40, 46, 50, 60, 62: Support substrate 26a, 28a: Support substrate edge 27, 31, 33a, 33b, 41, 45, 61, 64: Bending portion 30: Support portion side working electrode 32: Support portion Side counter electrode

Claims (3)

A biosensor manufacturing method for measuring the amount of a substrate component in a specimen,
A sensor unit having a working electrode and a counter electrode spaced apart from each other on a substrate made of an insulator, a reaction unit provided on the working electrode and the counter electrode, and a supply port for introducing a specimen to the reaction unit Forming process,
Forming a support portion on the support substrate made of an insulator and provided with a bent portion, by providing a support portion side working electrode and a support portion side counter electrode at a predetermined interval from the vicinity of the bent portion to the edge of the support substrate;
A mounting step of connecting the working electrode and the counter electrode to the support portion side working electrode and the support portion side counter electrode, respectively, and mounting the sensor portion on the support portion so as to intersect the bent portion;
A method for producing a biosensor, comprising: The step of forming the support portion includes a step of providing an opening in the support substrate at a position in contact with the bent portion or a position intersecting with the bent portion,
The biosensor manufacturing method according to claim 1, wherein, in the mounting step, the sensor unit is mounted on the support unit in a state where the supply port protrudes into the opening.   A biosensor manufactured by the manufacturing method according to claim 1.

Images