JP5237013B2 - Biosensor - Google Patents

Description

  The present invention relates to a biosensor capable of quantitative analysis by measuring the amount of a substrate component in a specimen.

  Conventionally, biosensors have been devised for measuring blood sugar levels and the like of specimens (see, for example, Patent Document 1 and Patent Document 2). According to this biosensor, the current between the working electrode for measurement and the counter electrode for measurement when the reaction part reacts to blood or the like introduced from the supply port in the state of being inserted into the attachment port of the measurement display. By measuring the value, blood glucose level and the like can be measured.

  However, since the conventional biosensor has a length of about several centimeters and is very small and flat, it has been difficult to remove the biosensor from a container on a desk, pick it with a finger, and attach it to a measurement display.

JP-A-8-320304 International Publication No. 2004/017057 Pamphlet

  Therefore, the inventor of the present invention has come to the present invention as a result of intensive studies to investigate the cause of such a problem and solve such a problem.

  That is, an object of the present invention is to provide a biosensor that can be easily held by a finger and attached to a measurement display.

The biosensor of the present invention includes a substrate having a formed Ri one end and the other end of an insulator, and measuring the working electrode provided on one end side on the substrate, the working electrode for the measurement at one end on the substrate A counter electrode for measurement provided at a predetermined interval, a reaction part provided on the measurement working electrode and the measurement counter electrode, a supply port for introducing a specimen to the reaction part, and the other substrate provided on the end side, and the connection terminal portions of the measuring working electrode and the measuring counter electrode, Ri consists mounted is inserted into the mounting opening of the measuring indicator, introducing said reaction portion from said supply port In the biosensor that measures the amount of the substrate component in the sample by reacting with the sample, a protrusion is provided on the front surface or the back surface between the reaction portion on the one end side and the connection terminal portion on the other end side. It has, or have a different protrusion shapes on the front and back surfaces And wherein the Rukoto.

In the biosensor of the present invention, a substrate having a formed Ri one end and the other end of an insulator, and measuring the working electrode provided on one end side on the substrate, for the measurement at one end on the substrate A counter electrode for measurement provided at a predetermined interval from the working electrode, a reaction part provided on the measurement working electrode and the counter electrode for measurement, a supply port for introducing a specimen to the reaction part, and the substrate provided on the other end side, and the connection terminal portions of the measuring working electrode and the measuring counter electrode, Ri consists mounted is inserted into the mounting opening of the measuring indicator, the reaction unit is the supply port In the biosensor that measures the amount of the substrate component in the sample by reacting with the sample introduced from the surface, the front surface or the back surface is deformed between the reaction portion on the one end side and the connection terminal portion on the other end side. To form protrusions or surfaces and Wherein the by deforming the surface to form a different protrusion shapes.

  According to the biosensor of the present invention, since the sensor part has a protrusion or can form a protrusion, the protrusion can be easily picked and held. For this reason, a diabetic patient etc. can pick up with a finger easily and can attach to a measurement display.

  Next, the biosensor according to the present invention will be described in detail based on the drawings. 1-3, the code | symbol 10 shows the biosensor of this invention.

  The biosensor 10 of the present invention includes a substrate 12 made of an insulator, a measurement working electrode 14 provided on the substrate 12, and a measurement working electrode 14 provided on the substrate 12 with a predetermined interval. A sensor unit 11 including a counter electrode 16, a measuring working electrode 14, a reaction unit 18 provided on the measuring counter electrode 16, and a supply port 20 for introducing blood (specimen) to the reaction unit 18; The biosensor is inserted and attached to the attachment port 52 of the measurement display 30 and measures the blood glucose level (substrate component amount) in the blood by the reaction unit 18 reacting to the blood introduced from the supply port 20. .

This biosensor 10 is configured to have a protrusion 58 on the surface 54 of the sensor unit 11 and to form a knob for hooking a finger when placed on the table top 60. In addition, a protrusion 58 is provided between the reaction portion 18 on the one end 19 side of the biosensor 10 and the connection terminal portion 23 on the other end 21 side. The projecting portion 58 has a quadrangular prism shape. When the projecting portion 58 is placed on the table top surface 60 with the projecting portion 58 facing upward, as shown in FIG. As shown in FIG. 2B, when the sensor is placed on the tabletop 60 with the projection 58 facing down, the sensor unit 11 forms a knob.

  The insulator constituting the substrate 12 is, for example, polyethylene terephthalate (PET), polyethylene naphthalate, a polyester resin sheet such as a biodegradable polyester resin composed of an aliphatic unit and an aromatic unit, more heat resistance, chemical resistance, Examples thereof include a polyamideimide sheet having excellent strength, a plastic sheet such as a polyimide film sheet, and an inorganic substrate such as ceramic.

  The measuring working electrode 14 and the measuring counter electrode 16 are formed on the electrically insulating substrate 12 by a good conductor such as platinum, gold, palladium, indium-tin oxide, or the like. As a forming method, hot stamping can be considered. Vacuum deposition or sputtering is preferable because a fine electrode pattern can be formed with high accuracy. In the case of sputtering, it can be formed at once by masking the outside of the formation of both electrodes.

  The reaction unit 18 includes an oxidoreductase and an electron acceptor. For example, it is configured by applying and drying a liquid material containing an oxidoreductase and an electron acceptor. Examples of the oxidoreductase include glucose oxidase when measuring glucose. Glucose oxidase reacts with glucose to produce gluconic acid and hydrogen peroxide. Moreover, when measuring an alcohol value, alcohol oxidase or alcohol dehydrogenase is used. When measuring lactic acid, lactate oxidase or lactate dehydrogenase is used. When measuring uric acid, uricase is used. Generally, it is an inorganic or organic fine powdery compound that promotes oxidation-reduction of enzymes. For example, alkali metal ferricyanide (particularly potassium ferricyanide is preferred), ferrocene or an alkyl-substituted product thereof, p-benzoquinone, methylene blue, potassium β-naphthoquinone-4-sulfonate, phenazine methosulfate, 2,6- Examples include dichlorophenol-indophenol. The alkali metal ferricyanide and ferrocene are stable as an electron transfer medium and are well soluble in aqueous solvents such as water, alcohols, or mixed solvents thereof, and thus effectively function as electron acceptors.

  The supply port 20 includes a front edge 31 of a cover 28 provided on the substrate 12 via a spacer 26 and a front edge 33 of the substrate 12. That is, the supply port 20 is configured by a gap generated between the leading edge 31 and the leading edge 33 by the spacer 26. The blood introduced from the supply port 20 can reach the tip edge 35 of the spacer 26 by capillary action if the amount is sufficient.

  As shown in FIG. 1, the biosensor 10 includes a measurement working electrode 14 and a measurement counter electrode 16 provided on a substrate 12, and a reaction portion 18 is applied on the measurement working electrode 14 and the measurement counter electrode 16. Then, it is formed by covering with a cover 28 via a spacer 26 and fixing a protruding portion 58 on the cover 28. Alternatively, the cover 28 and the projecting portion 58 are integrally formed, the measuring working electrode 14 and the measuring counter electrode 16 are provided on the substrate 12, and the reaction portion is provided on the measuring working electrode 14 and the measuring counter electrode 16. 18 is applied and the cover 28 and the protrusion 58 are fixed via the spacer 26.

  The measurement indicator 30 includes a fitting recess 60 for fitting the biosensor 10 including the protruding portion 58, and the biosensor 10 can be attached to the attachment port 52 so as to be energized, and the biosensor 10 is turned upside down. It is configured so that it cannot be inserted in the direction.

  The operation of measuring the blood glucose level using the biosensor 10 having such a configuration will be described below. As shown in FIG. 2, the blood glucose level measurer first takes the biosensor 10 out of the container and places it on the tabletop 60. The biosensor 10 has a length of about a few centimeters and is very small. When the biosensor 10 is placed on the table top surface 60 so as to roll from the container, the biosensor 10 is placed with the protruding portion 58 facing upward as shown in FIG. 2 and a case where it is placed with the protrusion 58 facing down, as shown in FIG. 2 (b). When placed with the protrusion 58 facing upward, the finger 62 can be easily hooked on the protrusion 58, and the protrusion 58 forms a knob. When placed with the protruding portion 58 facing down and the back surface 56 facing up, the finger 62 can be easily hooked on the sensor portion 11 and the sensor portion 11 forms a knob.

  If the knob 62 formed in this way is picked with the finger 62 to hold the biosensor 10 and the protruding portion 58 is turned up, it is inserted into the attachment port 52 as shown in FIG. Can be attached. At this time, when the biosensor 10 is in the reverse direction, since the sensor unit 11 interferes with the inner wall 62 of the fitting recess 60, the biosensor 10 cannot be inserted to the end and can be recognized as being in the reverse direction. If it recognizes that it is a front and back reverse direction, it can attach to the measurement display device 30 by making the protrusion part 58 into the front and back normal direction which becomes upper. For this reason, the biosensor 10 or the measurement display 30 is not damaged by trying to forcefully insert the biosensor 10 into the attachment port 52 many times.

  As mentioned above, although one Embodiment of this invention was described, this invention can be implemented also in another aspect. For example, the biosensor 70 shown in FIG. 4 may be used. The biosensor 70 has a protrusion 58 on the front surface 54 and a protrusion 72 on the back surface 56. According to this biosensor 70, the protrusion 58 forms a knob when placed on the table top 60 with the surface 54 side up, and the protrusion when the sample is placed on the table top 60 with the back 56 side up. 72 forms a knob. In addition, by changing the length of the protruding portion 58 and the length of the protruding portion 72, it is possible to prevent the front and back from being held in the opposite direction when visually attached to the attachment port 52 or by the feeling of the finger 62.

Moreover, the biosensor 90 shown in FIG. 5 may be used. The biosensor 90 has a protrusion 92 having a trapezoidal cross section on the surface 54. According to the biosensor 90, since the protruding portion 92 has a trapezoidal cross section, the finger 62 is more easily hooked on the protruding portion 92, and is easily picked.

Moreover, the biosensor 100 shown in FIG. 6 may be used. In this biosensor 100, a bent portion 102 is provided on the cover 28, and the bent portion 102 is bent upward to deform between the reaction portion 18 on one end 19 side and the connection terminal portion 23 on the other end 21 side. Thus, a protrusion can be formed on the surface 54. According to the biosensor 100, since the protruding portion is formed only at the time of use, it can be prevented from being bulky by being housed in a compact manner before use.

  Further, the present invention is not limited to the illustrated embodiment. For example, the biosensor of the present invention may be configured by fixing a protrusion to a conventional biosensor. In addition, the technical scope of the present invention includes embodiments in which various improvements, modifications, and variations are added based on the knowledge of those skilled in the art without departing from the spirit of the present invention. Moreover, you may implement with the form which substituted any invention specific matter to the other technique within the range which the same effect | action or effect produces.

  According to the biosensor of the present invention, the protruding portion can be picked and easily held. For this reason, it can be widely used as a biosensor attached to a measurement display.

It is a perspective view of the biosensor of the present invention. It is a perspective view which shows the use condition of the biosensor of FIG. 1, The figure (a) is a figure which shows the state mounted in the table top surface with the surface up, and the figure (b) has the back side up. It is a figure which shows the state mounted on the table | surface. It is a side view which shows the state which attaches the biosensor of FIG. 1 to a measurement display. It is a perspective view which shows other embodiment of the biosensor of this invention. It is a perspective view which shows other embodiment of the biosensor of this invention. It is a perspective view which shows other embodiment of the biosensor of this invention.

10, 70, 80, 90.100: Biosensor 12: Substrate 14: Measuring working electrode 16: Measuring counter electrode 18: Reaction part
19: One end 20: Supply port
21: The other end
23: Connection terminal portion 26: Spacer 28: Cover 30: Measurement display 54: Front surface 56: Back surface

Claims (2)

Provided spaced a substrate having a formed Ri one end and the other end of an insulator, and measuring the working electrode provided on one end side on the substrate, and the measurement the working electrode at one end on the substrate a predetermined distance The measurement counter electrode, the reaction working electrode and the reaction counter electrode provided on the measurement counter electrode, the supply port for introducing the specimen to the reaction electrode, and the other end of the substrate. a connection terminal portion of the measuring working electrode and the measuring counter electrode, Ri consists,
In the biosensor for measuring the amount of the substrate component in the sample by being inserted and attached to the attachment port of the measurement indicator, and the reaction part reacting to the sample introduced from the supply port,
A biosensor having a protruding portion on the front surface or the back surface, or a protruding portion having a different shape on the front surface and the back surface, between the reaction portion on the one end side and the connection terminal portion on the other end side . Provided spaced a substrate having a formed Ri one end and the other end of an insulator, and measuring the working electrode provided on one end side on the substrate, and the measurement the working electrode at one end on the substrate a predetermined distance The measurement counter electrode, the reaction working electrode and the reaction counter electrode provided on the measurement counter electrode, the supply port for introducing the specimen to the reaction electrode, and the other end of the substrate. a connection terminal portion of the measuring working electrode and the measuring counter electrode, Ri consists,
In the biosensor for measuring the amount of the substrate component in the sample by being inserted and attached to the attachment port of the measurement indicator, and the reaction part reacting to the sample introduced from the supply port,
Between the reaction part on the one end side and the connecting terminal part on the other end side, a protrusion is formed by deforming the front surface or the back surface, or a protrusion having a different shape by deforming the front surface and the back surface. Biosensor that can be formed.

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