JP5398684B2 - Biosensor - Google Patents

Description

  The present invention relates to a biosensor for measuring a specific component in a specimen.

  Conventionally, a biosensor has been devised for measuring a blood glucose level or the like of a specimen (see, for example, Patent Document 1). According to this biosensor, the blood glucose level or the like can be measured by measuring the current value between the working electrode and the counter electrode when the reaction part reacts with blood or the like introduced from the supply port. And this biosensor is attached to the sensor attachment opening part of a measurement display at the time of measuring a blood glucose level etc., and is removed from a measurement display and discarded after a measurement.

  Here, since the work of removing the biosensor from the measurement display after measurement was performed while holding the biosensor by a human hand, the blood adhering to the biosensor is infected with hepatitis C, human immunodeficiency syndrome, or the like. There is a risk, and the removal work of the biosensor has been performed with great care, such as wearing gloves. In particular, for an elderly person or a person who is not good at fine work with a fingertip, the work of attaching a small biosensor to a measurement display and the work of removing the biosensor are extremely difficult.

  Therefore, in view of the above problems, the biosensor can be easily attached to and detached from the measurement display, and the blood attached to the biosensor is difficult to adhere to the hand during the removal operation. Has been devised (see, for example, Patent Document 1).

  According to the biosensor disclosed in Patent Document 1, blood (sample) generated from a finger (specimen collection target) by sticking a needle and contacting the finger (sample) is brought into contact with the supply port of the biosensor to measure blood sugar level or the like. There is a need to do. However, it is difficult for the elderly and those who are not good at fine work with the fingertips to accurately contact the blood adhering to the finger to the small supply port, so it is still difficult to measure blood sugar levels. Sometimes it becomes.

JP 2009-14394 A

  Therefore, the inventor of the present invention has come to the present invention as a result of intensive studies to investigate the cause of such a problem and solve such a problem.

  An object of the present invention is to provide a biosensor that can easily and accurately contact a specimen that is generated from a specimen collection target and adheres to the specimen collection target with a supply port of the biosensor.

The biosensor of the present invention includes a substrate made of an insulator, a set of electrodes provided on the substrate, a reaction unit provided on the set of electrodes, and a supply for introducing a specimen to the reaction unit A biosensor that includes a mouth and an attachment portion that connects the pair of electrodes to a measurement display, and that performs measurement by bringing a sample that is generated from a sample collection target and attached to the finger into contact with the supply port there are, said comprising a position regulating means for regulating the position of the finger relative to the biosensor, the extending to the opposite side to the mounting portion to the reaction unit, comprising a knob plate to have a knob portion for pinching the knob plate Has a through-hole, the supply port projects into the through-hole, the knob plate has a folding line extending along the surface of the knob plate, and the tip side of the knob plate is folded at the folding line The supply port protrudes outward and the front Periphery of the through hole to form a protruding tip which projects towards the outside, projecting tip is characterized in that it constitutes the position regulating means.

  In the biosensor of the present invention, in the biosensor, the supply port is located on the rear end side of the knob plate with respect to the folding line of the knob plate, and the tip side of the knob plate is the folding line. By bending, the supply port is retracted to the rear end side with respect to the projecting front end portion.

  The biosensor of the present invention is characterized in that the biosensor includes a cover that covers the reaction part, and the knob plate is attached to the cover or the substrate.

  The biosensor of the present invention is characterized in that in the biosensor, the set of electrodes is located between the substrate and the knob plate.

  According to the biosensor of the present invention, the position of the sample collection target can be regulated by the position regulation unit. For example, the sample attached to the sample collection target can be brought into contact with the supply port while the sample collection target is brought into contact with the position regulation unit. Therefore, the position restricting means supports the sample collection target, so that the sample generated from the sample collection target and attached to the sample collection target can be easily and accurately brought into contact with the supply port of the biosensor.

It is a figure which shows the biosensor of this invention, the same figure (a) is a top view, The same figure (b) is an AA line | wire cut part sectional drawing. It is a figure for demonstrating the effect of the biosensor of FIG. 1, The figure (a) is sectional drawing of the state attached to the measurement indicator 22, and the figure (b) bent the knob | pick board 26. It is sectional drawing of a state. It is a figure for demonstrating the effect of the biosensor of FIG. 1, and is sectional drawing of the state which makes blood contact a supply port. It is a figure for demonstrating the effect of the biosensor of FIG. 1, and is a top view of the state which makes the blood contact a supply port. It is a top view which shows other embodiment of the biosensor of this invention.

  Next, the biosensor according to the present invention will be described in detail based on the drawings. 1-4, the code | symbol 10 is the biosensor which concerns on this invention.

  As shown in FIG. 1, the biosensor 10 according to the present invention includes a substrate 12 made of an insulator, a set of electrodes 14 provided on the substrate 12, and a reaction unit provided on the set of electrodes 14. 18, a supply port 20 for introducing blood (specimen) 100 to the reaction unit 18, and an attachment unit 24 for connecting the pair of electrodes 14 to the terminals of the measurement display 22, and a finger (sample collection target) 102. Is a biosensor that performs measurement by bringing the blood 100 that is generated from the blood and adhering to the finger 102 into contact with the supply port 20, and includes a position regulating means 104 that regulates the position of the finger 102 relative to the supply port 20. 1 to 3 are exaggerated and enlarged in the thickness direction of the biosensor 10 so that the positional relationship between the substrate 12 and the cover 34 can be understood.

  This biosensor 10 includes a knob plate 26 that extends to the opposite side of the attachment portion 24 with respect to the reaction portion 18 and has a knob portion 25 for picking, the knob plate 26 has a through hole 40, and the supply port 20 passes therethrough. Projecting into the hole 40. The knob plate 26 has a folding line L extending along the surface of the knob plate 26. By bending the tip 27 side of the knob plate 26 with the folding line L, the supply port 20 is moved to the outside as shown in FIG. In addition, the peripheral portion 106 of the through hole 40 forms a protruding tip portion 107 that protrudes outward, and the protruding tip portion 107 constitutes the position restricting means 104.

  Further, as shown in FIG. 1A, the supply port 20 is positioned on the rear end 44 side of the knob plate 26 with respect to the folding line L of the knob plate 26, and the tip 27 side of the knob plate 26 is indicated by the folding line L. By bending, the supply port 20 moves backward by the distance D from the projecting tip 107 to the rear end 44 side, so that the blood 100 attached to the finger 102 corresponds to the curved shape of the tip of the finger 102. The position of the finger 102 relative to the supply port 20 can be regulated while being in contact with. The knob plate 26 is attached to a cover 34 that covers the reaction unit 18, and the electrode 14 is positioned between the substrate 12 and the knob plate 26.

  The insulator constituting the substrate 12 is, for example, polyethylene terephthalate (PET), polyethylene naphthalate, a polyester resin sheet such as a biodegradable polyester resin composed of an aliphatic unit and an aromatic unit, more heat resistance, chemical resistance, Examples thereof include a polyamideimide sheet excellent in strength and the like, a plastic sheet such as a polyimide sheet, and an inorganic substrate such as ceramic.

  The set of electrodes 14 is configured by five electrode members arranged at intervals, and is attached to an electrode for measuring a blood glucose level, an electrode for detecting blood supply, and an attachment to the measurement display 22. It consists of electrodes for detection. However, the configuration is not particularly limited as long as the blood glucose level can be measured. The pair of electrodes 14 is formed of an electrode raw material that is a good conductor such as platinum, gold, nickel, palladium, indium-tin oxide, or the like. A method for forming the pair of electrodes 14 is not particularly limited. In the biosensor 10, a conductive layer made of an electrode raw material is formed on the surface of the substrate 12 by sputtering, and then formed into an electrode member shape by etching. Other forming methods may be printing or hot stamping.

  The reaction unit 18 includes an oxidoreductase and an electron acceptor on the vicinity of the tips of the pair of electrodes 14. For example, it is configured by applying and drying a liquid material containing an oxidoreductase and an electron acceptor. Examples of the oxidoreductase include glucose oxidase or glucose dehydrogenase when glucose is measured. Glucose oxidase reacts with glucose to produce gluconic acid and hydrogen peroxide. Glucose dehydrogenase reacts with glucose to produce gluconolactone. When measuring cholesterol, cholesterol oxidase and cholesterol esterase are used. Moreover, when measuring an alcohol value, alcohol oxidase or alcohol dehydrogenase is used. When measuring lactic acid, lactate oxidase or lactate dehydrogenase is used. When measuring uric acid, uricase is used. Examples of the electron acceptor include alkali metal ferricyanide (particularly potassium ferricyanide metal salt), ferrocene or an alkyl-substituted product thereof, p-benzoquinone, methylene blue, β-naphthoquinone-4-sulfonate potassium, and phenazine. Examples include methosulfate, 2,6-dichlorophenol-indophenol. Ferricyanide alkali metal salts and ferrocenes have a stable function as an electron transfer medium and are well soluble in aqueous solvents such as water, alcohols, or mixed solvents thereof, and thus effectively function as electron acceptors.

  As shown in FIG. 1B, the supply port 20 is provided on the substrate 12 via a spacer 32, and is formed by the tip 36 of the cover 34 that covers the reaction unit 18 and the tip 38 of the substrate 12. In other words, the supply port 20 is formed by a gap generated between the tip 36 and the tip 38 by the spacer 32. If the amount of the specimen such as blood 100 introduced from the supply port 20 is sufficient, it can reach the reaction unit 18 by capillary action.

  The folding line L of the knob plate 26 is constituted by a cut groove. As an alternative to this groove, a dotted line may be provided, or notches may be provided at both the left and right ends of the biosensor 10 in the length direction. Further, the knob plate 26 has a through hole 40, the supply port 20 projects into the through hole 40, and the supply port 20 is located on the tip 27 side of the knob plate 26 with respect to the folding line L of the knob plate 26. Therefore, by folding the tip 27 side of the knob plate 26 along the fold line L, the supply port 20 protrudes toward the outside, and blood can be attached to the supply port 20. The widths of the substrate 12, the spacer 32, and the cover 34 become narrower toward the supply port 20 so that the supply port 20 protrudes into the through hole 40. The knob plate 26 is attached to the cover 34 with a double-sided tape. The material of the knob plate 26 is not particularly limited, such as resin or paper, but polyethylene terephthalate (PET) that is easy to turn is preferable. Although the color of the biosensor of the present invention is not particularly limited, in the case of the biosensor 10, the substrate 12 is white and the knob plate 26 is translucent. For this reason, it is possible to distinguish between the picking plate 26 that is picked by hand and the substrate 12 that is not picked.

  The effect | action which measures a blood glucose level with such a biosensor 10 of this invention is demonstrated below.

  The biosensor 10 of the present invention is purchased in a state where it is put in a storage case, and when used, is taken out of the storage case and placed on a flat surface 28 of the desk. The biosensor 10 placed on the desk plane 28 or the like is held by the finger 30 being picked by the grip plate 26 and placed on the desk plane 28 as shown in FIG. The pair of electrodes 14 is inserted into the attachment port 42 of the display 22 and connected to a terminal (not shown) of the measurement display 22. Next, as shown in FIG. 2 (b), the biosensor 10 is folded around a fold line L by pressing the vicinity of the tip 27 of the knob plate 26 with the finger 30.

  By being folded along the folding line L, the supply port 20 of the biosensor 10 protrudes toward the outside. At this time, the peripheral portion 106 of the through hole 40 forms a protruding tip portion 107 that protrudes toward the outside, and the protruding tip portion 107 constitutes the position regulating means 104.

  Next, the finger 102 of the person who measures the blood glucose level is pierced with a needle and bleeded from the finger 102, and blood 100 adheres to the finger 102. The finger 102 to which the blood 100 has been attached is made to approach the supply port 20 while being slid substantially upward along the knob plate 26 in a state of being substantially horizontally oriented, and as shown in FIGS. Is stopped in contact with the supply port 20.

  At this time, the position of the finger 102 is regulated by the protruding tip portion 107, and in particular, the supply port 20 moves backward by the distance D from the peripheral portion 106 of the through hole 40 toward the rear end 44 side. Since the blood 100 adhering to the finger 102 can be brought into contact with the supply port 20 while being in contact with the finger 102, the protruding tip portion 107 supports the finger 102, so that the blood 100 can be easily and accurately supplied to the supply port 20 of the biosensor 10. Can be contacted. Also, the pressing force is not excessively applied to the supply port 20 of the biosensor 10 by the finger 102, and the vicinity of the supply port 20 of the biosensor 10 is not damaged.

  When blood 100 adheres to supply port 20 in this way, the blood glucose level is measured. When the blood glucose level measurement is completed, the biosensor 10 is removed by being pushed in the direction opposite to the insertion direction by an engagement member (not shown) in the measurement display 22 by sliding the discharge lever 23 with the finger 30. Alternatively, the pick plate 26 is picked by the finger 30 and is removed from the measurement display 22 and discarded.

  According to the biosensor 10 of the present invention, the protruding tip 107 constitutes the position restricting means 104 that restricts the position of the finger 102 to be sampled, so that the blood 100 is supplied to the supply port of the biosensor 10 as described above. 20 can be easily and accurately brought into contact. Further, since the biosensor 10 folds the grip plate 26 instead of the substrate 12 provided with the electrode 14, the electrode 14 is not damaged when folded. Further, since the biosensor 10 is formed by attaching the knob plate 26 to the cover 34, only the knob plate 26 can be appropriately changed to a shape that is easy to hold, for example, by increasing the thickness.

  As mentioned above, although one Embodiment of this invention was described, this invention can be implemented also in another aspect. For example, in the biosensor 10 of the present invention, as shown in FIG. 5, the folding line L of the knob plate 26 is formed in an oblique direction, and the knob plate 26 extends in a cross-sectional direction oblique to the direction in which the knob plate 26 extends toward the tip 27. It may be configured to be bent. Even if the folding line L is formed in an oblique direction, the supply port 20 protrudes outward by folding the tip 27 side of the knob plate 26 with the folding line L, and the peripheral portion 106 of the through hole 40 is exposed to the outside. A protruding tip portion 107 that protrudes toward the surface is formed, and the protruding tip portion 107 constitutes the position restricting means 104. In addition, the supply port 20 is positioned on the rear end 44 side of the knob plate 26 with respect to the folding line L of the knob plate 26, and the supply port 20 is protruded by bending the tip 27 side of the knob plate 26 with the folding line L. Since it moves backward by the distance D from the portion 107 to the rear end 44 side, the blood 102 attached to the finger 102 is brought into contact with the supply port 20 while the blood 100 attached to the finger 102 is in contact with the supply port 20 corresponding to the curved shape of the finger 102. The position can be regulated.

  In addition, the present invention can be implemented in a mode not shown. For example, the cover 34 may have a knob, or the cover 34 may be formed with the knob. That is, the cover 34 may be configured to also serve as the knob plate 26. In addition, the technical scope of the present invention includes embodiments in which various improvements, modifications, and variations are added based on the knowledge of those skilled in the art without departing from the spirit of the present invention. Moreover, you may implement with the form which substituted any invention specific matter to the other technique within the range which the same effect | action or effect produces.

  According to the biosensor of the present invention, by providing the position regulating means, it is possible to easily and accurately contact the specimen generated from the specimen collection target and attached to the specimen collection target with the supply port of the biosensor. For this reason, it can be widely used as a biosensor for measuring blood sugar levels and the like.

10: Biosensor 12: Substrate 14: Electrode 18: Reaction part 20: Supply port 22: Measurement display 23: Discharge lever 24: Mounting part 26: Knob plate 28: Plane 30: Finger 32: Spacer 34: Cover 40: Through Hole 42: Mounting port 44: Rear end L: Folding line
104: Position restricting means

Claims (4)

A substrate made of an insulator, a set of electrodes provided on the substrate, a reaction part provided on the set of electrodes, a supply port for introducing a specimen to the reaction part, and the set of A biosensor that includes a mounting portion for connecting an electrode to a measurement display, and performs measurement by bringing a specimen that is generated from a finger and attached to the finger into contact with the supply port,
A position restricting means for restricting the position of the finger with respect to the supply port;
Extending to the side opposite to the mounting portion with respect to the reaction unit, comprising a knob plate to have a knob portion for picking,
The knob plate has a through hole, and the supply port protrudes into the through hole,
The knob plate has a folding line extending along a surface of the knob plate;
By bending the tip end side of the knob plate along the fold line, the supply port protrudes outward, and a peripheral portion of the through hole protrudes outward to form a protruding tip. A biosensor whose part constitutes the position regulating means.   The supply port is located on the rear end side of the knob plate with respect to the folding line of the knob plate, and the supply port is more than the protruding tip portion by bending the tip side of the knob plate with the folding line. The biosensor according to claim 1, wherein the biosensor retracts toward the rear end side. The biosensor according to claim 1, further comprising a cover that covers the reaction unit, wherein the knob plate is attached to the cover or the substrate. The biosensor according to any one of claims 1 to 3, wherein the set of electrodes is located between the substrate and the knob plate.

Images