KR102153518B1 - Blood sugar bio sensor - Google Patents

Abstract

A blood sugar biosensor is disclosed. The blood glucose biosensor is printed with a hydrophobic material on the electrode layer disposed in the first region of the paper film, the second region of the paper film and the electrode layer other than the first region, and a protective film layer in which a predetermined region into which blood is injected is opened. It is disposed inside a predetermined open area and on the electrode layer, and may include an enzyme layer printed with an enzyme that reacts with blood. Accordingly, fabrication of the blood sugar biosensor can be performed simply and quickly.

Description

Blood sugar biosensor {BLOOD SUGAR BIO SENSOR}

The present invention relates to a blood sugar biosensor, and more particularly, to a blood sugar biosensor implemented on a paper film using a printing technology.

In modern times, environmental factors such as high calorie, high fat, high protein diet, lack of exercise, stress, and other environmental factors have been greatly affected by the westernization of diet, and the number of diabetic patients is rapidly increasing every year, and according to this trend, technology that can diagnose health It is being regarded as important.

Meanwhile, the biosensor is a device for analysis that combines a biological element and a physical and chemical detector, and can be used to search for an analyte. One of the most common commercial biosensors is the blood sugar biosensor.

The conventional electrochemical blood sugar biosensor has a three-layer structure consisting of a lower plate on which electrodes are printed, a middle plate to form a channel, and a middle plate and an upper plate to bond the upper plate to the lower plate. The manufacturing process is complicated because each layer must be manufactured and adhered while accurately aligning. Actually, due to the problem of reproducibility of the manufacturing process, a difference in output characteristics of the blood sugar sensor strip may occur, and the manufacturing process is bound to be complicated to solve this problem.

Therefore, there is a need for a blood sugar biosensor capable of accurately measuring blood sugar while a manufacturing process is simple.

Existing electrochemical blood sugar biosensors are mostly made of plastic films, so there is a problem of environmental pollution. As the problem of seawater and drinking water pollution caused by fine plastic particles has been raised, a move to limit the use of household plastics is rapidly progressing around Europe.

Therefore, there is a need for an eco-friendly blood sugar biosensor that does not use a plastic film.

Meanwhile, the above information is only presented as background information to aid understanding of the present invention. No decision has been made and no argument is made as to whether or not any of the above is applicable as the prior art for the present invention.

Registered Patent Publication No. 10-1171457 (Registration Date: 2012.7.31)

The present invention has been devised to solve the above-described problems, and an embodiment of the present invention in detail proposes a blood sugar biosensor implemented on a paper film using a printing technology.

The technical problems to be achieved in the present invention are not limited to the technical problems mentioned above, and other technical problems that are not mentioned will be clearly understood by those of ordinary skill in the technical field to which the present invention belongs from the following description. I will be able to.

A blood glucose biosensor according to an embodiment of the present invention for realizing the above object includes an electrode layer disposed in a first area of a paper film; A passivation layer printed with a hydrophobic material on the electrode layer and the second area of the paper film other than the first area, and opening a predetermined area into which blood is injected; And an enzyme layer disposed inside an opened predetermined area and on the electrode layer, and on which an enzyme reacting with the blood is printed.

More specifically, it further comprises an enzyme protective covering layer for protecting the enzyme layer, wherein the enzyme protective covering layer is a region in which a predetermined end of the paper film is extended, and the extended region is the paper around a specific line Can be superimposed on film.

A blood sugar biosensor according to another embodiment of the present invention for realizing the above-described problems includes an electrode layer disposed in a first area of a paper film; A passivation layer printed with a hydrophobic material, formed to open an area into which blood is injected, and directly adjacent to the first area; An enzyme layer disposed inside the opened area and on the electrode layer and printed with an enzyme that reacts with the blood; And an adhesive layer that is printed on a part of the passivation layer and bonds the passivation layer and the first area when the passivation layer is superimposed on the first area around a specific line.

In another embodiment of the present invention for realizing the above-described problems, a blood sugar biosensor includes an electrode layer having electrodes printed on a plurality of first regions included in one paper film; A passivation layer corresponding to each of the plurality of first regions, and in which a hydrophobic material is printed on a predetermined region of each of the plurality of second regions arranged to overlap each of the plurality of first regions one-to-one; An enzyme layer printed on a specific end region of each of the plurality of electrode layers; And an adhesive layer that is printed on a portion of each of the plurality of second regions and adheres to each of the first regions corresponding to each of the plurality of second regions, wherein the enzyme layer is disposed on the plurality of protective film layers. An open area may be provided in the formed area.

In another embodiment of the present invention for realizing the above-described problem, a blood sugar biosensor includes an electrode layer disposed in a first area of a paper film; An enzyme layer printed on the electrode layer and reacting with blood; A second region adjacent to one end of the first region, including a blood inlet, and in which the enzyme layer is opened when overlapping with the first region; A third region adjacent to the other end of the first region and including a vent, wherein the second region overlaps the first region with a first specific line as a center, and the third region forms a second specific line It may overlap with the first area and the third area as the center.

According to various embodiments of the present invention, the following effects can be derived.

First, since a blood sugar biosensor can be manufactured in several printing processes, a blood sugar biosensor can be manufactured remarkably simply, and sensor manufacturing can be performed quickly.

Second, since several blood sugar biosensors can be simultaneously manufactured through a simple printing process, the blood sugar biosensors can be manufactured inexpensively and quickly.

Third, it is eco-friendly because a paper film is used instead of a plastic film generally used for blood sugar biosensors.

The effects that can be obtained in the present invention are not limited to the effects mentioned above, and other effects not mentioned can be clearly understood by those of ordinary skill in the art from the following description. will be.

1(a) to 1(d) are views schematically illustrating a process of manufacturing a blood glucose biosensor according to an embodiment of the present invention.
2(a) to 2(e) are diagrams for explaining a manufacturing process of a blood glucose biosensor according to an embodiment of the present invention.
3 is a cross-sectional view of a blood sugar biosensor according to an embodiment of the present invention.
4 to 9 are diagrams for explaining a blood sugar biosensor according to various embodiments of the present disclosure.
As described above, the present invention has been illustrated and described with reference to a preferred embodiment, but is not limited to the above-described embodiment, and within the scope not departing from the spirit of the present invention, to those of ordinary skill in the art. Various changes and modifications will be possible.

Hereinafter, various embodiments of the present invention will be described in more detail with reference to the accompanying drawings. However, in describing the present invention, when it is determined that a detailed description of a related known function or configuration may unnecessarily obscure the subject matter of the present invention, a detailed description thereof will be omitted.

1(a) to 1(d) are views schematically illustrating a process of manufacturing a blood glucose biosensor blsua 100 according to an embodiment of the present invention. It can be largely summarized into three processes.

First, an electrode layer, a protective layer, and an enzyme layer constituting a blood sugar biosensor may be designed through a personal computer (PC) or the like. For example, a plurality of layers constituting the biosensor may be designed through a computer aided design (CAD) program (FIG. 1(a)). The above design is automated and can be performed without user manipulation.

Then, an electrode layer, a protective layer, and an enzyme layer designed through a printer can be printed (FIGS. 1(b) and 1(c)). Inkjet printing technology may be applied to the printing. Materials necessary for printing the electrode layer, the protective layer, and the enzyme layer may be included in the ink.

Finally, the plurality of printed layers are dried, and the blood sugar biosensor blsua 100 may be completed by a cutting process (FIG. 1(d)).

Hereinafter, a blood sugar biosensor 100 according to an exemplary embodiment will be described. 2(a) to 2(e) are views for explaining a manufacturing process of the blood glucose biosensor 100 according to an embodiment of the present invention, and FIG. 3 is a blood glucose biosensor according to an embodiment of the present invention It is a cross-sectional view of (100).

The paper film 110 shown in FIGS. 2A to 3 may be a paper or a paper film. However, in implementation, the paper film 110 may be replaced with a flat plastic film. The paper film 110 may be implemented in the size of the blood sugar biosensor 100 from the initial process, and may be prepared in the size of a plurality of blood sugar biosensors and manufactured in the size of the blood sugar biosensor through a cutting process.

Referring to FIG. 2B, electrode layers e and 120 may be disposed in a predetermined area on the paper film 110. The electrode layer 120 may be disposed on a partial area of the paper film 110, and the electrode layer 120 may include a working electrode 120A and a reference electrode 120B. Here, when measuring the electrode potential generated between the solution and the metal, the reference electrode 120B is a necessary electrode because its absolute value cannot be measured.

The electrode layer 120 may be manufactured by a printing process using conductive ink. Inkjet printing technology may be used for the printing process, and screen printing technology and dispensing technology may be used depending on embodiments.

When printing is performed, the printer can be automatically driven, and when printing the electrode layer 120, the material for printing the electrode layer 120 is automatically output to the printing outlet and printed on the paper film 110. Can be.

In addition, the electrode layer 120, in addition to the working electrode 120A and the reference electrode 120B, is a strip recognition electrode for determining that a blood glucose biosensor strip is inserted into a measurement system (body) in a state capable of measuring, and blood is measured. It may further include a blood recognition electrode for determining whether or not it is applied in a possible state, and in implementation, more various electrodes may be printed on the electrode layer 120.

Referring to FIG. 2(c), a protective film layer disposed on the electrode layer 120, in which a hydrophobic material is printed on a predetermined area of the paper film 110, and a predetermined area into which blood is injected is opened (punched). 130) can be deployed. A part of the passivation layer 130 may be disposed on the electrode layer 120, and another part may be disposed on the paper film 110. The passivation layer 130 may be printed with a hydrophobic ink to serve as an insulating layer.

In addition, the protective film layer 130 has one purpose to protect the electrode, and the protective film layer 130 has another purpose for applying blood only to the electrode layer 120 on which the enzyme layer 140 (to be described later) is printed. .

Referring to FIG. 2D, the enzyme layer 140 may be disposed inside of the open area of the passivation layer 130 and on the electrode layer 120, and is a layer on which enzymes reacting with blood are printed. The enzyme may be a glucose enzyme, but when implemented, it may be implemented with various substances.

A printer performing the processes of FIGS. 2A to 2D may automatically perform the above processes according to the process.

Fig. 2(e) shows a cross-sectional view of Fig. 2(d) in the X to X” direction. The blood sugar biosensor 100 includes a paper film 110, an electrode layer 120, a protective layer 130, and an enzyme layer 140.

Referring to FIG. 3, blood (blo) may react with the enzyme layer, and blood glucose may be measured according to a reaction such as oxidation at the electrode of the electrode layer 120. In the blood sugar biosensor 100 according to an embodiment of the present invention, the upper blood application method in which the sensor is applied by directly contacting the electrode on the electrode is applied, thereby injecting blood using a capillary phenomenon through an existing narrow channel User convenience may be improved.

4 to 9 are diagrams for explaining a blood sugar biosensor according to various embodiments of the present disclosure. Hereinafter, different blood sugar biosensors will be described with reference to each drawing.

First, FIG. 4 shows a blood sugar biosensor 100 according to an embodiment of the present invention.

The blood glucose biosensor 100 disclosed in FIG. 4 may further include an enzyme protective cover (hou, 150). The enzyme protection cover 150 is a cover that protects the enzyme layer 140, and an area extending a predetermined end of the paper film 110 may be superposed on the paper film 110 with a specific line (fol) as the center. . Accordingly, the enzyme layer 140 is protected to prevent contamination or loss of the enzyme layer when handling the blood sugar biosensor.

Next, FIG. 5 shows a blood sugar biosensor 100 according to an embodiment of the present invention.

The blood sugar biosensor 100 may be manufactured in a folding format using a single sheet of paper film. The first area on which the electrode layer 120 is disposed and the second area on which the protective layer 130 is printed may be printed on one paper film. The first area and the second area may be disposed directly adjacent to each other.

The passivation layer 130 may be printed with a hydrophobic material to prevent the introduction of the hydrophilic material, and may be formed to be opened (punched) in the areas where blood is injected (win, 170).

The enzyme layer 140 is disposed inside the opened area and on the electrode layer 120, and is a layer on which an enzyme reacting with blood is printed.

In addition, when the adhesive layer 180 is printed on a part of the passivation layer 130, and the second area including the passivation layer 130 overlaps the first area and a specific line, The first region and the second region may be bonded. Here, the adhesive layer 180 may also be disposed using a printing technique, and various adhesive materials may be applied.

6 and 7 illustrate fabrication of a plurality of blood sugar biosensors according to an embodiment of the present invention in an assembling method at the same time.

Blood sugar biosensors can be manufactured simultaneously with one paper film. To this end, an electrode layer may be printed on a plurality of first regions included in one paper film 15. A protective layer on which a hydrophobic material is printed may be disposed in the second areas corresponding to each of the first areas on which the electrode layers are printed, respectively. In addition, an enzyme layer may be disposed in each of the first regions. After printing is finished, a plurality of blood sugar biosensors can be manufactured by origami.

Adhesive layers 180A to 180D for bonding each of the plurality of second regions and each of the first regions corresponding to each of the plurality of second regions may be printed on a portion of each of the plurality of second regions, and a plurality of openings Areas 170A to 170E may also be disposed. The plurality of opening regions is a region in which blood reacts and corresponds to a region for opening the enzyme layer.

8 and 9 illustrate a blood glucose biosensor 100 according to an embodiment of the present invention. The blood sugar biosensor 100 described herein corresponds to the blood sugar biosensor 100 using a capillary phenomenon. 8 illustrates manufacturing of one blood sugar biosensor, and FIG. 9 illustrates manufacturing of a plurality of blood sugar biosensors.

First, referring to FIG. 8, one film may be divided into first to third regions, and an electrode layer and an enzyme layer on a portion of the electrode layer may be disposed in the first region. The second region may be overlapped with the first region, and may include an opening through which the enzyme layer is opened, and the third region may be adjacent to the other end of the first region and may include a vent.

The opening through which the enzyme layer is opened may be indicated as a channel, and the vent may be indicated as a pore.

First, the second area may overlap with the first area around a first specific line, and the third area may overlap with the first area and the third area around the second specific line.

9 illustrates a method of simultaneously fabricating a blood sugar biosensor using a capillary phenomenon using one film. According to FIG. 9, a total of eight blood sugar biosensors can be simultaneously manufactured.

On the other hand, this specification is not intended to limit the present invention to the specific terms presented. Accordingly, although the present invention has been described in detail with reference to the above-described examples, those skilled in the art can make modifications, changes, and modifications to these examples without departing from the scope of the present invention. The scope of the present invention is indicated by the claims to be described later rather than the detailed description, and all changes or modified forms derived from the meaning and scope of the claims and their equivalent concepts should be interpreted as being included in the scope of the present invention. do.

Claims (5)

As a blood sugar biosensor,
An electrode layer disposed on the first area of the paper film and printed by a printer using ink;
A protective film layer printed using the printer with a hydrophobic material on the electrode layer and the second region of the paper film other than the first region, and having a predetermined region into which blood is injected into the upper direction; And
It is disposed on a part of the electrode layer disposed inside a predetermined opened area, and an enzyme that reacts with the blood is printed using the printer, and includes an enzyme layer disposed with an open top,
The blood is injected from the upper direction of the enzyme layer, blood sugar biosensor. The method of claim 1,
Further comprising an enzyme protective cover layer for protecting the enzyme layer,
The enzyme protective cover layer,
A blood sugar biosensor in which a predetermined end of the paper film is an extended area, and the extended area is superimposed on the paper film around a specific line. As a blood sugar biosensor,
An electrode layer disposed in the first area of one paper film and printed by a printer using ink;
A passivation layer printed using the printer with a hydrophobic material, formed to open an area into which blood is injected, and directly adjacent to the first area;
When the passivation layer and the first region overlap a specific line, they are disposed on a part of the electrode layer disposed inside the opened region, and the enzyme reacting with the blood is printed using the printer, The enzyme layer is arranged to be opened; And
A portion of the passivation layer is printed using the printer, and when the passivation layer is superimposed on the first area around the specific line, the passivation layer includes an adhesive layer bonding the passivation layer and the first area,
The blood is injected from the upper direction of the enzyme layer, blood sugar biosensor. As a blood sugar biosensor,
An electrode layer in which electrodes are printed on a plurality of first areas included in one paper film using a printer using ink;
The hydrophobic material is disposed in a predetermined area of each of the plurality of second areas corresponding to each of the plurality of first areas and arranged to overlap each of the plurality of first areas in a one-to-one manner, and a predetermined area into which blood is injected is opened. A protective layer printed using a printer;
When each of the plurality of first regions and the second region corresponding thereto are superimposed, printing is performed using the printer on a specific end region of each of the plurality of electrode layers disposed inside the opened predetermined region, Enzyme layer; And
Including; an adhesive layer for bonding each of the first regions corresponding to each of the plurality of second regions, which are printed using the printer on a portion of each of the plurality of second regions,
The blood is injected from the upper direction of each of the enzyme layers, a blood sugar biosensor. As a blood sugar biosensor,
An electrode layer disposed on a first area of one paper film and printed using a printer using ink;
An enzyme layer printed using the printer on the electrode layer and reacting with blood;
A second region adjacent to one end of the first region, including a blood inlet, and in which the enzyme layer is opened when overlapping with the first region; And
Includes; a third area adjacent to the other end of the first area and including a vent,
The blood sugar biosensor, wherein the second area overlaps with the first area around a first specific line, and the third area overlaps with the first area and the third area around a second specific line.

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